Nano carbon dot antibacterial and nose-refreshing gel as well as preparation method and application of nano carbon dot antibacterial and nose-refreshing gel

Abstract

The invention relates to nano carbon dot antibacterial and nose-refreshing gel as well as a preparation method and application thereof. The nano carbon dot antibacterial and nose-refreshing gel is prepared from paulownia flower nano carbon dots, konjac glucomannan, hyaluronic acid, antibacterial peptide, a mint extract, borneol, carbomer, sea salt water and sodium hydroxide. Compared with the prior art, the nano carbon dot antibacterial and nose-refreshing gel is smeared on the nasal cavity and Yingxiang acupoint, can effectively improve the symptoms of unsmooth drainage, unsmooth breathing and the like caused by inflammation in the nasal cavity and nasal mucosa hyperemia and swelling, can also inhibit the breeding of bacteria in the nasal cavity, and is an excellent medical material.

Description

Technical Field

[0001] The present invention relates to the field of biomedicine technology, and in particular to a nano-carbon dot antibacterial nasal gel and a preparation method and application thereof. Background Art

[0002] Inflammation of the nose usually refers to inflammation of the nasal mucosa caused by allergens, bacteria, and viruses. It can usually be divided into three types: allergic rhinitis, acute rhinitis, and chronic rhinitis. Most patients with allergic rhinitis develop symptoms in spring and autumn, mainly due to allergies to pollen and mites, and will have symptoms such as a large amount of clear nasal discharge, nasal itching, nasal congestion, and sneezing. Such patients need to stay away from allergens, wear masks when going out in daily life, and prevent contact with allergens, and usually the symptoms can be relieved. Acute rhinitis is usually caused by infection with pathogens such as bacteria and viruses in the upper respiratory tract, that is, colds, and patients will have symptoms such as runny nose, sneezing, nasal itching, and nasal congestion. Chronic rhinitis often recurs, and the treatment is slow to take effect, and the treatment effect varies from person to person. At present, the three types of rhinitis can only be controlled by treatment, but it cannot be cured, which has a serious impact on the quality of life and social economy of patients. Therefore, it is necessary to take active and effective prevention and control measures at all levels of medical and health care, and antibacterial nasal gel, as a convenient and fast biological dressing, can effectively alleviate the clinical distress of patients and greatly improve their quality of life.

[0003] Nanotechnology is a highly interdisciplinary subject, and its research content is intertwined with various disciplines, which has led to the rapid development of nanotechnology. In recent years, nanomaterials have become one of the hot topics in the field of biological sciences. [1] Carbon quantum dots are a type of carbon nanomaterial that has emerged in recent years. They have natural carbon properties and excellent optical properties, making them a rising star in nanomaterials. [2-5] Carbon quantum dots were discovered accidentally during the separation and purification of single-walled carbon nanotubes. Xu et al. initiated follow-up research in 2004, using the fluorescent properties of carbon quantum dots to create a new class of viable fluorescent nanomaterials. [6] This fluorescent carbon nanomaterial was given the name "carbon quantum dots" (hereinafter referred to as CQDs) in 2006, and Sun et al. proposed a synthetic route to produce strong fluorescent emission CQDs through surface passivation. [7] , with the advantages of simple and fast operation. CQDs are usually spherical structures, less than 10nm in size and exhibit strong and stable fluorescence. What is more gratifying is that they can be made from cheap and readily available raw materials through a simple, easy and fast synthesis method. As a special type of quantum dots, they have excellent fluorescence properties and adjustable surface chemical properties, and have attracted much attention in biomedical fields such as bioimaging, disease diagnosis and treatment. [8] .

[0004] Paulownia flower is the flower of Paulownia or Paulownia tomentosa, a plant of the Scrophulariaceae family. It tastes spicy and bitter, is slightly cold in nature, and enters the lung meridian. It has the effects of dispelling wind and heat, drying dampness and stopping dysentery, relieving cough and asthma, and clearing the liver and improving eyesight. It can be used as an auxiliary treatment for a variety of respiratory infections and other diseases. In addition, modern applied research shows that the total flavonoids of Paulownia flower range from 100 to 0.39 mg·mL -1 The concentration has a significant antibacterial effect on Staphylococcus aureus. [9] However, there is no related research on the antibacterial properties of paulownia flower carbon dots.

[0005] Konjac glucoside (hereinafter referred to as KGM) is a natural high molecular weight polysaccharide extracted from konjac. It is a relatively abundant natural renewable resource after starch and cellulose. The special structure of KGM gives it very excellent properties, such as strong water absorption, high biocompatibility, good film-forming property, and biodegradability.

[10] However, KGM usually needs to be modified and mixed with other natural or biodegradable

[10] , thereby improving the mechanical properties and antibacterial properties of KGM. The U.S. Food and Drug Administration (FDA) considers KGM to be a safe fiber material

[11] .

[0006] Hyaluronic acid (HA), also known as hyaluronic acid, is a high molecular weight linear polysaccharide. It is a high molecular weight polymer formed by repeated alternation of N-acetylglucosamine and glucuronic acid through β-1,4 and β-1,3 glycosidic bonds. HA is a rigid spiral column configuration in space, and the inner side of the column has a strong hydrophilicity due to a large number of light groups. It is a naturally degradable biomedical material whose degradation products can be absorbed by the body, and it is widely used in clinical practice.

[0007] Antimicrobial peptides originally refer to a class of alkaline polypeptide substances with antimicrobial activity that are induced in insects. They have a molecular weight of about 2000 to 7000 and are composed of 20 to 60 amino acid residues. Antimicrobial peptides have attracted widespread attention due to their broad-spectrum antimicrobial activity, rapid bactericidal effect, low toxicity, and low resistance to drug resistance. In addition, the natural structure of antimicrobial peptides also indicates that they are easily degradable and have good biocompatibility.

[0008] Peppermint extract is a brown powder extracted from the aerial part of the Lamiaceae plant, Pu He. Its chemical composition: contains volatile oil, mainly I-menthol, 1-menthol and menthol esters. Studies have shown that the minimum inhibitory concentration (MIC) of peppermint extract against Bacillus subtilis, Staphylococcus aureus and Escherichia coli is 60.0μL / mL, 30μL / mL and 20μL / mL respectively.

[12] .

[0009] The references described in the above background technology are as follows:

[0010] [1] Lim SY, Shen W, Gao ZQ. Carbon quantum dots and their applications. Chem Soc Rev, 2015, 44: 362-381.

[0011] [2] Dong X, Su Y, Geng H, Li Z, Yang C, Li X, Zhang Y. Fast one-stepsynthesis of n-doped Carbon dots by pyrolyzing ethanolamine. J Mater Chem C, 2014, 2(36): 7477-7481.

[0012] [3]Hou J, Yan J, Zhao Q, Li Y, Ding H, Ding L.Anovel one-pot route for large-scale preparation of highly photoluminescent carbon quantum dotspowders. Nanoscale, 2013, 5(20):9558-9561.

[0013] [4]Song YB, Zhu SJ, Yang B. Bioimaging based on fluorescent carbondots. RSC Adv, 2014, 4(52): 27184-27200.

[0014] [5]Zhai YL, Zhu ZJ, Zhu CZ, Ren JT, Wang EK, Dong SJ. Multifunctional water-soluble luminescent carbon dots for imaging and Hg 2+sensing.JMater ChemB,2014,2(40):6995-6999.

[0015] [6] Xu XY, Ray R, Gu YL, Ploehn HJ, Gearheart L, Raker K, Scrivens WA. Electrophoretic analysis and purification of fluorescent single-walledcarbon nanotube fragments. J Am Chem Soc, 2004, 126(40): 12736-12737.

[0016] [7]Sun YP, Zhou B, Lin Y, Wang W, Fernando KAS, Pathak P, Meziani MJ, Harruff BA, Wang X, Wang HF, Luo PG, Yang H, Kose ME, Chen BL, Veca LM, XieSY. Quantum-sized carbon dots for bright and colorful photoluminescence. JAm Chem Soc,2006,128(24):7756-7757.

[0017] [8] Yang Lijuan, Dai Fan, Shao Shilong, et al. Frontier progress of fluorescent carbon quantum dots in biomedical research[J]. Chinese Journal of Lasers, 2024, 51(03): 185-196.

[0018] [9] Liu Lu, Fu Jiayang, Deng Hongxia. Study on ultrasonic-assisted extraction of total flavonoids from Paulownia flowers and its antibacterial activity[J]. Journal of Northeast Agricultural University, 2022, 53(07): 57-66.

[0019]

[10] FENG Y, LI

[0020]

[11] Tang JY, Chen JL, Guo J, et al. Construction and evaluation of fibrillar composite hydrogel of collagen / konjac glucomannan for potential biomedical Applications. Regenerative Biomaterals, 2018, 239–250.

[0021]

[12] Kang Liansen, Xing Xiaoke, Wei Shiqian. Study on the antibacterial activity of peppermint extract[J]. Journal of Nanyang Normal University, 2013, 12(06): 21-23. Summary of the invention

[0022] The purpose of the present invention is to provide a nano-carbon dot antibacterial and nasal awakening gel and its preparation method and application. The present invention provides an original nano-carbon dot antibacterial and nasal awakening gel containing paulownia flower nano-carbon dots, konjac glucoside, hyaluronic acid, antimicrobial peptides, mint extract, borneol, carbomer, and sea salt water through compounding in different proportions. The dynamic viscosity of the nano-carbon dot antibacterial and nasal awakening gel obtained by the preparation method of the present invention is ≥10cP.

[0023] The purpose of the present invention can be achieved by the following technical solutions:

[0024] A nano-carbon dot antibacterial and nasal gel, comprising, by weight percentage:

[0025]

[0026] Furthermore, the nano carbon dots are paulownia flower nano carbon dots, and the specific preparation method is as follows:

[0027] S1. Adding paulownia flowers and lysine into water, and stirring to obtain a mixture;

[0028] S2, subjecting the mixture obtained in step S1 to a hydrothermal reaction to obtain a reaction solution;

[0029] S3, centrifugally filtering the reaction solution obtained in step S2, and drying to obtain paulownia flower nanocarbon dots.

[0030] Furthermore, in step S1, the mass ratio of the paulownia flower to lysine is (10-1): (1-10).

[0031] Furthermore, in step S1, paulownia flower: water = 1 g: 5-50 mL.

[0032] Furthermore, in step S2, the hydrothermal reaction temperature is 160-200° C., preferably 200° C., and the hydrothermal reaction time is 8-48 h, preferably 24 h.

[0033] Furthermore, in step S3, the centrifugal speed is 5000-10000 r / min, and the centrifugal time is 10-30 min.

[0034] Furthermore, in step S3, the drying temperature is 60-80° C., and the drying time is 12-24 hours.

[0035] Furthermore, the konjac glucoside polysaccharide is formed by connecting D-glucose residues and D-mannose residues with β-1,4 glycosidic bonds at a molecular ratio of 1:1.6, and the molar ratio of mannose to glucose is 1.5-1.7. The konjac glucoside polysaccharide has good water absorption and absorbability and degradability.

[0036] Furthermore, the antimicrobial peptide molecular formula is C 114 H 180 N 30 O 29 The amino acid sequence of the antimicrobial peptide is shown in SEQ ID NO.1, the SCAS number is 108433-95-0, and the MDL number is MFCD00133522.

[0037] As a preferred technical solution, the SEQ ID NO.1 is as follows:

[0038] H-Gly-lle-Gly-Lys-Phe-Leu-His-Ser-Ala-Lys-Lys-Phe-Gly-Lys-Ala-Phe-Val-Gly-Glu-ll e-Met-Asn-Ser-OH.

[0039] Furthermore, the mint extract is light brown powder with a special mint aroma.

[0040] Furthermore, the aqueous phase is a sea salt solution, and the mass fraction of the sea salt solution is 5 to 22%.

[0041] The present invention also provides a method for preparing a nano-carbon dot antibacterial nasal gel, the specific steps of which are as follows:

[0042] S1, preparing nano carbon dots;

[0043] S2, dissolving the nano carbon dots, konjac glucoside, hyaluronic acid and antimicrobial peptide obtained in step S1 in an aqueous phase to prepare a solution A;

[0044] S3, mixing peppermint extract, borneol and carbomer, and dissolving them in an aqueous phase to prepare a gel solution B;

[0045] S4. Mix the solution A obtained in step S2 and the gel solution B obtained in step S3 to obtain a gel solution, and adjust the pH to obtain the nano-carbon dot antibacterial and nasal refreshing gel.

[0046] Furthermore, in step S2, the mass ratio of the nano-carbon dots, konjac glucoside, hyaluronic acid and antimicrobial peptide obtained in step S1 is (0.1-1): (0.1-1): (0.1-1): (0.1-0.5).

[0047] Furthermore, in step S3, the mass ratio of the peppermint extract, borneol and carbomer is (0.1-1):(0.1-1):(1-10).

[0048] Furthermore, in step S4, the mass ratio of the solution A obtained in step S2 to the gel solution B obtained in step S3 is (0.1-1):(1-10).

[0049] Furthermore, in step S4, the pH is adjusted to neutral, and the pH adjusting agent is a sodium hydroxide solution.

[0050] Furthermore, after step S4, step S5 is provided, in which the nano-carbon dot antibacterial and nasal refreshing gel obtained in step S4 is put into a packaging bottle with an anti-reflux type extrusion pump head, sealed and packaged, and sterilized by irradiation.

[0051] In addition, the present invention also provides a use of a nano-carbon dot antibacterial nasal gel in the preparation of a medicine for preventing and / or treating rhinitis.

[0052] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0053] The nano-carbon dot antibacterial and nasal gel of the present invention combines bioactive ingredients such as paulownia flower nano-carbon dots, konjac glucoside, hyaluronic acid, and antimicrobial peptides, and has higher antibacterial performance after composite modification. It can quickly relieve symptoms such as poor drainage and poor breathing caused by congestion and swelling of the nasal mucosa in the nasal cavity and Yingxiang acupoint, and can also effectively inhibit the growth of bacteria in the nasal cavity.

[0054] The nano-carbon dot antibacterial nasal gel was tested for its in vitro inhibitory effect on the growth of Escherichia coli and Staphylococcus aureus using the filter paper method. The experimental results showed that it had certain inhibitory activity against the two tested bacteria and was an excellent medical antibacterial material.

[0055] The nano carbon dot antibacterial nasal gel of the present invention contains paulownia flower nano carbon dots, antibacterial peptides, peppermint extract and other biologically active antibacterial ingredients, which can quickly and effectively inhibit pathogens such as bacteria and viruses; in addition, borneol is good at moving around and opening the mind, and has the effect of awakening the nose; konjac glucoside, hyaluronic acid and sea salt water have the effect of quickly absorbing nasal fluid. The organic combination of several ingredients can quickly relieve the symptoms of poor drainage and poor breathing caused by rapid congestion and swelling of the nasal mucosa in the nasal cavity and Yingxiang acupoint, and effectively inhibit the growth of bacteria in the nasal cavity. It is a convenient and fast antibacterial nasal gel for rhinitis. The antibacterial nasal gel can be prepared into various dosage forms and can be widely used in clinical and daily health care of patients with rhinitis. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 This is an infrared spectrum of the Paulownia flower nano-carbon dots prepared in Example 1 of the present invention. DETAILED DESCRIPTION

[0057] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

[0058] In the following examples, the sources of the reagents are as follows:

[0059] Paulownia flowers were purchased from Zhongkangtang Technology Co., Ltd., model 250g;

[0060] Lysine was purchased from Maclean, model 100 g;

[0061] Konjac glucoside was purchased from Maclean, model 25 g;

[0062] Hyaluronic acid was purchased from McLean, model 5 g;

[0063] Antimicrobial peptides were purchased from Maclean, model 5 g;

[0064] Peppermint extract was purchased from Changqing Biotechnology Co., Ltd., model 100g;

[0065] Borneol was purchased from Aladdin, model 25 g;

[0066] Carbomer was purchased from Maclean, model 100 g;

[0067] Sea salt solution was purchased from Hubei Nuozhongsheng Pharmaceutical Co., Ltd., with a model of 500 mL.

[0068] In conjunction with the accompanying drawings, some embodiments of the present invention are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments may be combined with each other.

[0069] Example 1

[0070] This embodiment provides a method for preparing a nano-carbon dot antibacterial nasal gel, and the specific steps are as follows:

[0071] S1. Preparation of Paulownia Flower Nano-carbon Dots:

[0072] Mix 1.0g of Paulownia flower and 0.75g of lysine, add 15mL of purified water, stir at room temperature until uniform, then transfer the mixture to a 25mL polytetrafluoroethylene-lined hydrothermal reactor, place it in a constant temperature drying oven and heat it at 200℃ for 24h. After the reaction is completed, slowly cool to room temperature at a speed of 10℃ / min. Take out and centrifuge the reaction solution at a speed of 10000r / min for 10min, filter the obtained clear solution with a 0.22um filter membrane, and vacuum dry the obtained solution at 60℃ to obtain Paulownia flower nanocarbon dots, which are stored at 4℃ for later use.

[0073] S2, dissolving 0.2 g of the paulownia flower nanocarbon dots obtained in step S1, 0.2 g of konjac glucoside, 0.5 g of hyaluronic acid and 0.3 g of antimicrobial peptide in 10 mL of 9% sea salt solution, mixing and stirring until uniformly dispersed, to prepare solution A;

[0074] S3, dissolving 0.1 g of peppermint extract, 0.5 g of borneol, and 5.0 g of carbomer in 100 mL of 9% sea salt solution, mixing and stirring until uniformly dispersed, to prepare a gel solution B;

[0075] S4, pouring the solution A obtained in step S2 and the gel solution B obtained in step S3 into a beaker at a mass ratio of 1:8, and then mixing and stirring until uniformly dispersed to obtain a gel solution, adding 0.1 mmol / mL sodium hydroxide solution to adjust the pH to neutral, and obtaining the nanocarbon dot antibacterial and nasal gel.

[0076] S5. Put the obtained nano-carbon dot antibacterial and nasal refreshing gel obtained in step S4 into a packaging bottle with an anti-reflux squeeze pump head, seal it, and sterilize it by irradiation.

[0077] like Figure 1 As shown in the infrared spectrum of Paulownia flower nano-carbon dots, 3743 cm -1 The stretching vibration peak of -OH is 2928cm -1 and 1394cm -1 It is attributed to the characteristic absorption peak of CH, 2361 cm -1 and 2234cm -1 =CH stretching vibration peak, 1646cm -1 and 1516cm -1Vibration peaks appeared near -NH-C=O and COO - The characteristic absorption peak of 1030cm -1 The corresponding absorption peak is the characteristic absorption peak of COC. This indicates that there are a large number of hydroxyl and carboxyl functional groups on the surface of the synthesized paulownia flower nano-carbon dots, which makes the carbon dots highly water-soluble.

[0078] Example 2

[0079] A nano-carbon dot antibacterial nasal gel and a preparation method thereof, the specific steps are as follows:

[0080] S1. Preparation of Paulownia Flower Nano-carbon Dots:

[0081] Mix 1.0g of Paulownia flower and 0.75g of lysine, add 10mL of purified water, stir at room temperature until uniform, then transfer the mixture to a 25mL polytetrafluoroethylene-lined hydrothermal reactor, place it in a constant temperature drying oven and heat it at 180°C for 48h. After the reaction is completed, slowly cool to room temperature at a speed of 10°C / min. Take out and centrifuge the reaction solution at a speed of 8000r / min for 15min, filter the obtained clear solution with a 0.22um filter membrane, and vacuum dry the obtained solution at 60°C to obtain Paulownia flower nanocarbon dots, which are stored at 4°C for later use.

[0082] S2, dissolving 0.3 g of the paulownia flower nanocarbon dots obtained in step S1, 0.1 g of konjac glucoside, 0.4 g of hyaluronic acid and 0.3 g of antimicrobial peptide in 10 mL of 9% sea salt solution, mixing and stirring until uniformly dispersed, to prepare solution A;

[0083] S3, dissolving 0.2 g of peppermint extract, 1.0 g of borneol, and 10.0 g of carbomer in 100 mL of 9% sea salt solution, mixing and stirring until uniformly dispersed, to prepare a gel solution B;

[0084] S4, pouring the solution A obtained in step S2 and the gel solution B obtained in step S3 into a beaker at a mass ratio of 1:8, and then mixing and stirring until uniformly dispersed to obtain a gel solution, adding 0.1 mmol / mL sodium hydroxide solution to adjust the pH to neutral, and obtaining the nanocarbon dot antibacterial and nasal gel.

[0085] S5. Put the obtained nano-carbon dot antibacterial and nasal refreshing gel obtained in step S4 into a packaging bottle with an anti-reflux squeeze pump head, seal it, and sterilize it by irradiation.

[0086] Example 3

[0087] A nano-carbon dot antibacterial nasal gel and a preparation method thereof, the specific steps are as follows:

[0088] S1. Preparation of Paulownia Flower Nano-carbon Dots:

[0089] Mix 1.0g of Paulownia flower and 0.75g of lysine, add 10mL of purified water, stir at room temperature until uniform, then transfer the mixture to a 25mL polytetrafluoroethylene-lined hydrothermal reactor, place it in a constant temperature drying oven and heat it at 190°C for 36h. After the reaction is completed, slowly cool to room temperature at a speed of 10°C / min. Take out and centrifuge the reaction solution at a speed of 6000r / min for 20min, filter the obtained clear solution with a 0.22um filter membrane, and vacuum dry the obtained solution at 60°C to obtain Paulownia flower nanocarbon dots, which are stored at 4°C for later use.

[0090] S2, dissolving 0.4 g of the paulownia flower nanocarbon dots obtained in step S1, 0.1 g of konjac glucoside, 0.5 g of hyaluronic acid and 0.3 g of antimicrobial peptide in 10 mL of 9% sea salt solution, mixing and stirring until uniformly dispersed, to prepare solution A;

[0091] S3, dissolving 0.2 g of peppermint extract, 1.0 g of borneol, and 10.0 g of carbomer in 100 mL of 9% sea salt solution, mixing and stirring until uniformly dispersed, to prepare a gel solution B;

[0092] S4, pouring the solution A obtained in step S2 and the gel solution B obtained in step S3 into a beaker at a mass ratio of 1:8, and then mixing and stirring until uniformly dispersed to obtain a gel solution, adding 0.1 mmol / mL sodium hydroxide solution to adjust the pH to neutral, and obtaining the nanocarbon dot antibacterial and nasal gel.

[0093] S5. Put the obtained nano-carbon dot antibacterial and nasal refreshing gel obtained in step S4 into a packaging bottle with an anti-reflux squeeze pump head, seal it, and sterilize it by irradiation.

[0094] Example 4

[0095] A nano-carbon dot antibacterial nasal gel and a preparation method thereof, the specific steps are as follows:

[0096] S1. Preparation of Paulownia Flower Nano-carbon Dots:

[0097] Mix 1.0g of Paulownia flower and 0.5g of lysine, add 10mL of purified water, stir at room temperature until uniform, then transfer the mixture to a 25mL polytetrafluoroethylene-lined hydrothermal reactor, place it in a constant temperature drying oven and heat it at 160°C for 48h. After the reaction is completed, slowly cool to room temperature at a speed of 10°C / min. Take out and centrifuge the reaction solution at a speed of 5000r / min for 30min, filter the obtained clear solution with a 0.22um filter membrane, and vacuum dry the obtained solution at 80°C to obtain Paulownia flower nanocarbon dots, which are stored at 4°C for later use.

[0098] S2, dissolving 0.5 g of the paulownia flower nanocarbon dots obtained in step S1, 0.2 g of konjac glucoside, 0.4 g of hyaluronic acid and 0.3 g of antimicrobial peptide in 10 mL of a 22% by mass sea salt solution, mixing and stirring until uniformly dispersed, to prepare a solution A;

[0099] S3, dissolving 0.3 g of peppermint extract, 1.0 g of borneol, and 10.0 g of carbomer in 100 mL of 22% sea salt solution, mixing and stirring until uniformly dispersed, to prepare a gel solution B;

[0100] S4, pouring the solution A obtained in step S2 and the gel solution B obtained in step S3 into a beaker at a mass ratio of 1:8, and then mixing and stirring until uniformly dispersed to obtain a gel solution, adding 0.1 mmol / mL sodium hydroxide solution to adjust the pH to neutral, and obtaining the nanocarbon dot antibacterial and nasal gel.

[0101] S5. Put the obtained nano-carbon dot antibacterial and nasal refreshing gel obtained in step S4 into a packaging bottle with an anti-reflux squeeze pump head, seal it, and sterilize it by irradiation.

[0102] Comparative Example 1

[0103] This comparative example provides an antibacterial nasal gel and a preparation method thereof, and the specific steps are as follows:

[0104] S1, dissolving 0.2 g of konjac glucoside, 0.4 g of hyaluronic acid and 0.03 g of antimicrobial peptide in 10 mL of 22% sea salt solution, mixing and stirring until uniformly dispersed, to prepare solution A;

[0105] S2, dissolving 0.3 g of peppermint extract, 1.0 g of borneol, and 10.0 g of carbomer in 100 mL of 22% sea salt solution, mixing and stirring until uniformly dispersed, to prepare a gel solution B;

[0106] S3, pouring the solution A obtained in step S1 and the gel solution B obtained in step S2 into a beaker at a mass ratio of 1:8, and then mixing and stirring until uniformly dispersed to obtain a gel solution, adding 0.1 mmol / mL sodium hydroxide solution to adjust the pH to neutral, and obtaining an antibacterial and refreshing nasal gel.

[0107] S4. Put the obtained nano-carbon dot antibacterial and nasal refreshing gel obtained in step S3 into a packaging bottle with an anti-reflux squeeze pump head, seal it, and sterilize it by irradiation.

[0108] Performance Testing

[0109] The performance of the nano-carbon dot antibacterial nasal gel prepared in Examples 1-4 was tested, and the test results are as follows:

[0110] 1. pH value detection

[0111] Table 1 pH value of nano carbon dot antibacterial nasal gel

[0112] Example 1 Example 2 Example 3 Example 4 pH 6.2 6.5 6.4 6.7

[0113] As shown in Table 1, the pH value of the nano-carbon dot antibacterial and nasal refreshing gel prepared in Examples 1 to 4 is close to the physiological environment of the human body, which helps the gel to better penetrate the skin barrier without causing irritation and damage to the skin.

[0114] 2. Dynamic viscosity of nano-carbon dot antibacterial nasal gel

[0115] Table 2 Dynamic viscosity values ​​of nano-carbon dot antibacterial nasal gel

[0116] Example 1 Example 2 Example 3 Example 4 Dynamic viscosity (cP) 402600 413400 414600 432600

[0117] As shown in Table 2, the dynamic viscosity values ​​of the nano-carbon dot antibacterial nasal-awakening gels prepared in Examples 1 to 4 are relatively large, indicating that the nano-carbon dot antibacterial nasal-awakening gels have low fluidity, which is beneficial to the adhesion of the nano-carbon dot antibacterial nasal-awakening gels at fixed positions, thereby better exerting the effects of the nano-carbon dot antibacterial nasal-awakening gels.

[0118] 3. Antibacterial performance test

[0119] The antibacterial activity of the nano-carbon dot antibacterial nasal gel prepared in Examples 1 to 4 and the antibacterial nasal gel prepared in Comparative Example 1 was determined by the filter paper method. The specific steps are as follows:

[0120] In the clean bench, pour the high temperature and high pressure sterilized solid culture medium (liquid at this time) into the culture dish quickly, take out the preserved bacteria from the refrigerator, streak, and wait for a few days (depending on the growth situation). After the bacterial culture is completed, transfer the two test bacteria to the liquid culture medium at a 2% inoculation rate, shake at 37℃ for 12 hours for use.

[0121] Take 100 μL of Escherichia coli and Staphylococcus aureus suspension respectively on the sterilized culture medium, and coat the entire culture medium with the bacterial liquid. Insert the filter paper into the sample (the nanocarbon dot antibacterial and nasal awakening gel prepared in Examples 1 to 4) and soak it. When taking it out, stay for a while to remove the excess solution, and then place the filter paper on the culture medium. The filter paper soaked with the antibacterial and nasal awakening gel prepared in Comparative Example 1 is used as a control. Three parallel experiments were performed for each antibacterial and nasal awakening gel sample. At 25°C, observe the experimental results after culturing for 24 hours, measure the diameter of the antibacterial circle, and take the average value. The experimental results are shown in Table 3.

[0122] Table 3 Antibacterial performance test results of nano carbon dot antibacterial nasal gel in different embodiments

[0123]

[0124] As shown in Table 3, the nano-carbon dot antibacterial nasal gel prepared in Examples 1 to 4 and the antibacterial nasal gel prepared in Comparative Example 1 were tested for their in vitro inhibitory effects on the growth of Escherichia coli and Staphylococcus aureus using the filter paper method. The experimental results show that the nano-carbon dot antibacterial nasal gel prepared in Examples 1 to 4 has a certain inhibitory effect on both bacteria. Compared with the antibacterial nasal gel prepared in Comparative Example 1, the diameters of the antibacterial circles of both bacteria are increased, indicating that after the addition of nano-carbon dots, the inhibitory effect of the sample is enhanced. Therefore, the nano-carbon dot antibacterial nasal gel prepared by the present invention has good antibacterial performance and is an excellent medical antibacterial material.

[0125] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A nano-carbon dot antibacterial nasal gel, characterized in that: In terms of mass percentage, its components include:

2. The nano-carbon dot antibacterial nasal gel according to claim 1, characterized in that: The nano carbon dots are paulownia flower nano carbon dots, and the specific preparation method is as follows: S1. Adding paulownia flowers and lysine into water, and stirring to obtain a mixture; S2, subjecting the mixture obtained in step S1 to a hydrothermal reaction to obtain a reaction solution; S3, centrifugally filtering the reaction solution obtained in step S2, and drying to obtain paulownia flower nanocarbon dots.

3. The nano-carbon dot antibacterial nasal gel according to claim 2, characterized in that: In step S1, the mass ratio of the paulownia flower to lysine is (10-1): (1-10); Paulownia flower: water = 1g: 5-50mL.

4. The nano-carbon dot antibacterial and nasal gel according to claim 2, characterized in that: In step S2, the hydrothermal reaction temperature is 160-200° C., and the hydrothermal reaction time is 8-48 hours; In step S3, the centrifugal speed is 5000-10000 r / min, and the centrifugal time is 10-30 min; The drying temperature is 60-80° C., and the drying time is 12-24 hours.

5. The nano-carbon dot antibacterial nasal gel according to claim 1, characterized in that: The konjac glucoside is formed by connecting D-glucose residues and D-mannose residues by β-1,4 glycosidic bonds at a molecular ratio of 1:1.6, and the molar ratio of mannose to glucose is 1.5-1.7; The antimicrobial peptide molecular formula is C 114 H 180 N 30 O 29 The amino acid sequence of the antimicrobial peptide is shown in SEQ ID NO.

1.

6. The nano-carbon dot antibacterial and nasal gel according to claim 1, characterized in that: The water phase is a sea salt solution, and the mass fraction of the sea salt solution is 5-22%.

7. A method for preparing the nano-carbon dot antibacterial nasal gel as claimed in any one of claims 1 to 6, characterized in that: The specific steps are as follows: S1, preparing nano carbon dots; S2, dissolving the nano carbon dots, konjac glucoside, hyaluronic acid and antimicrobial peptide obtained in step S1 in an aqueous phase to prepare a solution A; S3, mixing peppermint extract, borneol and carbomer, and dissolving them in an aqueous phase to prepare a gel solution B; S4. Mix the solution A obtained in step S2 and the gel solution B obtained in step S3 to obtain a gel solution, and adjust the pH to obtain the nano-carbon dot antibacterial and nasal refreshing gel.

8. The method for preparing a nano-carbon dot antibacterial nasal gel according to claim 7, characterized in that: In step S2, the mass ratio of the nano-carbon dots, konjac glucoside, hyaluronic acid and antimicrobial peptide obtained in step S1 is (0.1-1): (0.1-1): (0.1-1): (0.1-0.5); In step S3, the mass ratio of the peppermint extract, borneol and carbomer is (0.1-1):(0.1-1):(1-10).

9. The method for preparing a nano-carbon dot antibacterial nasal gel according to claim 7, characterized in that: In step S4, the mass ratio of solution A obtained in step S2 to gel solution B obtained in step S3 is (0.1-1):(1-10); The pH is adjusted to neutral, and the pH adjusting agent is a sodium hydroxide solution.

10. Use of the nano-carbon dot antibacterial and nasal-invigorating gel as claimed in any one of claims 1 to 6 in the preparation of a drug for preventing and / or treating rhinitis.

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