High-quality and high-efficiency culture method for cut-flower lily cotyledon

Abstract

The invention provides a high-quality and high-efficiency culture method for cut-flower lily cotyledons. The high-quality and high-efficiency culture method for the cut-flower lily cotyledon comprises the following steps of sterilization, pre-culture, slicing, tissue culture, regeneration plant obtaining, re-culture, soil fertility increasing, soil disinfection and cut-flower lily cultivation. According to the method, the new buds in the bulbs of the cut-flower lily are sliced, the slices are subjected to tissue culture, then a large number of cut-flower lily regenerated plants are generated and cultivated, then the regenerated plants are cultivated in soil, and the excellent characters of female parents can be reserved while the scale is enlarged.

Description

Technical Field

[0001] The invention belongs to the field of plant cultivation, and in particular relates to a high-quality and efficient cultivation method for cut lily bulblets. Background Art

[0002] As one of the most popular flowers in the current flower market, the large-scale cultivation of cut lilies has always been an urgent problem to be solved, especially when a cut lily with extremely excellent traits and beautiful appearance appears. If it is cultivated simply through a few bulbs of the cut lily, the scale cannot be expanded, and if it is cultivated through different lily bulbs, the high-quality traits of the lily cannot be guaranteed. Summary of the invention

[0003] In view of the shortcomings of the existing technology of cultivating cut lilies, that the scale of planting through natural bulb division cannot be expanded, and that the high-quality traits of lilies cannot be retained when cultivating different lily bulbs, the present invention provides a high-quality and efficient cultivation method for cut lily bulblets, which is achieved by slicing new buds inside the bulbs of cut lilies and performing tissue culture propagation to produce a large number of cut lily regenerated plants for cultivation, thereby expanding the scale and retaining the excellent traits of the original species.

[0004] A high-quality and efficient culture method for cut lily bulblets comprises the following steps: S1: Sterilization Select the bulbs of cut lilies, place them in a fungicide diluted 1000-1200 times, soak for 10-30 minutes, take them out, and wash them with running water; S2: Pre-culture The cut lily bulbs treated in S1 were transferred to the preculture medium and precultured for 3-4 months; S3: Slice The outer scales of the pre-cultured cut lily bulbs in S2 are removed, the middle scales are taken out, and then the bulbs are cut into slices with a thickness of 2 to 3 mm; the slices include root disks and basal roots.

[0005] S4: Tissue Culture Take one slice from S3 and place it in the induction medium for tissue induction culture for 16-25 days, with the temperature controlled at 20-30°C and the light intensity controlled at 3000-4000lx; S5: Obtaining regenerated plants The induced slices in S4 are placed in a growth medium and cultured for 1-2 months, with the temperature controlled at 20-30°C and the light intensity controlled at 3000-4000 lx to obtain cut lily regenerated plants; S6: Recultivation of rooted bulblets The cut lily regenerated plants obtained in S5 are cultured in growth culture according to their different diameters, and the culture is continued to obtain cut lily rooted bulblets of uniform size; S7: Soil fertilization After peat and perlite are mixed in a mass ratio of 3:1 to prepare a soil matrix, polyacrylamide and decomposed organic fertilizer are added and stirred evenly to obtain a fertilized soil matrix; S8: Soil disinfection The soil substrate after fertilization in S7 is sterilized by steam to obtain a cultivation substrate.

[0006] S9: Daughter sphere matrix culture Spread the cultivation substrate in S8 at the set position with a thickness of ≥5 cm. Do not compact the substrate during the spreading process. Then plant the rooted bulbs of cut lilies in S6 evenly on the substrate at set intervals, cover with 4-5 cm of soil, and perform temperature control, watering, topdressing and shading management in the later stage.

[0007] As one aspect of the present invention, the cut lily bulbs in step S1 are cut lily bulbs without disease spots and mechanical damage on the surface.

[0008] As one aspect of the present invention, the temperature of the pre-culture in step S2 is 20-30°C.

[0009] As one aspect of the present invention, the illumination intensity during the pre-culture in step S2 is 3000-4000 lx.

[0010] As one aspect of the present invention, the method for preparing the preculture medium in step S2 comprises the following steps: mixing MS medium, 80 g / L sucrose and 7 g / L agar, adding 1 g / L citric acid to adjust the pH to 5.0-6.0, and sterilizing to obtain a preculture medium.

[0011] As one aspect of the present invention, the method for preparing the induction medium in step S4 includes the following steps: mixing MS medium, 2 mg / L6-BA, 0.1 mg / LNAA, 30 g / L sucrose and 7 g / L agar, adding 1 g / L citric acid to adjust the pH to 5.0-6.0, and sterilizing to obtain the induction medium.

[0012] As one aspect of the present invention, the method for preparing the growth medium in S5 comprises the following steps: mixing 1 / 2MS medium, 0.5 mg / L6-BA, 0.3 mg / LNAA, 0.2 mg / L IBA, 30 g / L sucrose and 7 g / L agar, adding 1 g / L citric acid to adjust the pH to 5.0-6.0, sterilizing, and obtaining the growth medium.

[0013] As one aspect of the present invention, a plurality of sieve plates are arranged in the steam sterilization reactor in step S8, and the soil matrix is ​​poured in from the top sieve plate and continuously screened, during which steam at 50-60° C. is introduced to sterilize the soil matrix.

[0014] As one aspect of the present invention, in the process of uniformly mixing the decomposed organic fertilizer and the steam-sterilized soil matrix in step S6, polyacrylamide and organic matter are added to the soil matrix, and the organic matter comes from the original soil where the cut lily is located in step S1.

[0015] As one aspect of the present invention, the present invention also includes transplanting the cut lily regeneration plant, and the specific steps are as follows: T1: The culture medium obtained in S8 is divided into culture bottles, and the culture medium occupies 2 / 5 of the space of the culture bottle, and the culture bottle is made of transparent material; T2: Slowly add 10wt% sterile water of the culture medium inside the culture bottle into the culture bottle along the wall of the culture bottle, and then let it stand for 10-15 minutes; T3: The cut lily regenerated plants in S5 were cultivated in the cultivation medium of the culture bottle, the temperature was controlled at 18-22°C, the light intensity was simulated to the real light intensity, and the cultivation was carried out for 2-3 days; T4: Take out the cut lily regenerated plants cultured in T3, spread the cultivation medium in S8 at the set position without compacting, and then evenly cultivate the obtained cultured cut lily regenerated plants on the cultivation medium at set intervals, and perform temperature control, watering, topdressing and shading management in the later stage.

[0016] As one aspect of the present invention, the training of cut lily regenerated plants is also included, which specifically includes the following steps: after the cut lily regenerated plants are transplanted and cultured for 2-3 days at T3, the temperature is controlled to be continuously reduced within one day according to the temperature reduction curve, and the cut lily regenerated plants are cultured for one day, wherein the temperature reduction curve uses time as an independent variable, and the temperature decreases smoothly from 12-18°C to 0-3°C; then the temperature is controlled to be continuously increased within one day according to the temperature increase curve, and the cut lily regenerated plants are continued to be cultured for one day, wherein the temperature increase curve uses time as an independent variable, and the temperature increases smoothly from 12-18°C to 30-37°C; and dead cut lily regenerated plants are removed during the training period.

[0017] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. The present invention slices the new buds inside the bulb of cut lily and performs tissue induction culture to produce a large number of cut lily regenerated plants for cultivation, thereby expanding the scale and retaining the excellent traits of the mother plant.

[0018] 2. The present invention continuously screens the soil matrix so that the soil matrix is ​​in a dispersed state, thereby increasing the contact area between the soil matrix and steam and improving the disinfection effect on the soil matrix.

[0019] 3. The addition of polyacrylamide in the present invention can increase the pores inside the soil matrix and improve the air permeability inside the soil matrix. The addition of organic matter can not only increase the pores inside the soil matrix, but also enrich the food web inside the soil matrix, which is convenient for the cultivation of cut lilies.

[0020] 4. The present invention simulates the optimal growth conditions of cut lilies and performs transplanting and cultivation on the regenerated plants of cut lilies, so that the regenerated plants of cut lilies grow more roots and improve the survival rate of the regenerated plants of cut lilies during cultivation.

[0021] 5. When the cut lily regenerated plants are transplanted and cultivated, the present invention simulates extreme temperature conditions to exercise the cut lily regenerated plants and eliminate dead cut lily regenerated plants, thereby avoiding the death of the cut lily regenerated plants during actual cultivation and wasting manpower and material costs.

[0022] 6. The high-quality and efficient cultivation method of cut lilies of the present invention can obtain high-quality lily bulbs with a diameter of 10 to 12 cm after one growth cycle (about 6 months). DETAILED DESCRIPTION

[0023] The present invention is further described in detail below in conjunction with specific embodiments.

[0024] Example 1 A high-quality and efficient culture method for cut lily bulblets comprises the following steps: S1: Sterilization Select the bulbs of cut lilies without disease spots and mechanical damage on the surface, place them in a 1000-fold diluted fungicide, soak for 20 minutes, take them out, and wash them with running water; S2: Pre-culture The cut lily bulbs treated in S1 are transferred to a preculture medium and precultured for 3 months. The preparation method of the preculture medium in step S2 comprises the following steps: mixing MS culture medium, 80 g / L sucrose and 7 g / L agar, adding 1 g / L citric acid to adjust the pH to 5.8, and sterilizing to obtain.

[0025] The pre-culture medium is a high-sugar and high-osmotic culture medium, which can reduce the water content inside the cut lily bulb and avoid the inactivation of the cut lily bulb; S3: Slice The outer scales of the cut lily bulbs pre-cultured in S2 are removed, the middle scales are taken out, and then cut into slices with a thickness of 2 to 3 mm; the slices also include the root disk and basal roots under the middle scales.

[0026] Each slice can produce one or more plants, which is convenient for large-scale cultivation of cut lilies; S4: Tissue Culture A slice in S3 is placed in an induction medium for tissue induction culture for 24 days, the temperature is controlled at 20°C, and the light intensity is controlled at 3500 lx. The preparation method of the induction medium in step S4 comprises the following steps: mixing MS medium, 2 mg / L 6-BA, 0.1 mg / L NAA, 30 g / L sucrose and 7 g / L agar, adding 1 g / L citric acid to adjust the pH to 5.8, and sterilizing to obtain; S5: Obtaining regenerated plants The induced slices in S4 are placed in a growth medium, cultured for 2 months, the temperature is controlled at 20°C, and the light intensity is controlled at 3500 lx, to obtain cut lily regenerated plants, wherein the preparation method of the growth medium in S5 comprises the following steps: 1 / 2MS medium, 0.5 mg / L 6-BA, 0.3 mg / L NAA, 0.2 mg / L IBA, 30 g / L sucrose and 7 g / L agar are mixed, and then 1 g / L citric acid is added to adjust the pH to 5.8, and sterilized to obtain; S6: Recultivation of rooted bulblets The cut lily regenerated plants obtained in S5 are cultured in growth medium according to their diameters, and cultured continuously to obtain cut lily rooted bulblets of uniform size; S7: Soil fertilization Peat and perlite are mixed in a mass ratio of 3:1 to prepare a soil matrix, decomposed organic fertilizer is added to the soil matrix, and the decomposed organic fertilizer and the soil matrix are evenly stirred, and polyacrylamide and organic matter are added to the soil matrix to obtain a fertilized soil matrix; the organic fertilizer comes from the native soil where the cut lily is located in step S1, and the organic matter includes microorganisms, microbial products and some animals in the soil, such as earthworms, to obtain the fertilized soil matrix, and the addition of polyacrylamide can increase the pores in the soil matrix and improve the air permeability in the fertilized soil matrix, and the addition of organic matter can not only increase the pores in the soil matrix after fertilization, but also enrich the food web in the soil matrix, so as to facilitate the cultivation of the cut lily; S8: Soil disinfection Then, the fertilized soil matrix is ​​steam sterilized in a sterilization reactor, wherein a plurality of sieve plates are arranged in the sterilization reactor, and the fertilized soil matrix is ​​poured in from the uppermost sieve plate and continuously screened, during which 50° C. steam is introduced to sterilize the fertilized soil matrix, thereby obtaining a cultivation matrix; By continuously screening the cultivation substrate, the cultivation substrate is kept in a dispersed state, increasing the contact area between the cultivation substrate and steam, and improving the disinfection effect of the cultivation substrate; S9: Daughter sphere matrix culture The cultivation substrate in S8 was spread flat at the set position with a thickness of ≥5 cm. No compaction was performed during the spreading process to avoid reducing the pores inside the soil substrate. The rooted bulbs of cut lilies in S6 were evenly planted on the substrate at set intervals and covered with 4-5 cm of soil. Temperature control, watering, topdressing and shading management were performed in the later stage. The survival rate of cut lilies reached 78.5%.

[0027] The invention slices the new buds inside the bulb of the cut lily and performs tissue induction culture to produce a large number of cut lily regenerated plants for cultivation, thereby expanding the scale and retaining the excellent properties of the original species.

[0028] Example 2 A high-quality and efficient culture method for cut lily bulblets comprises the following steps: S1: Sterilization Select the bulbs of cut lilies without disease spots and mechanical damage on the surface, place them in a 1000-fold diluted fungicide, soak for 20 minutes, take them out, and wash them with running water; S2: Pre-culture The cut lily bulbs treated in S1 are transferred to a preculture medium and precultured for 3 months. The preparation method of the preculture medium in step S2 comprises the following steps: mixing MS culture medium, 80 g / L sucrose and 7 g / L agar, and then adding 1 g / L citric acid to adjust the pH to 5.8. The preculture medium is a high-sugar hypertonic culture medium, which can reduce the water content inside the cut lily bulbs and avoid inactivation of the cut lily bulbs; S3: Slice The outer scales of the cut lily bulbs pre-cultured in S2 are removed, the middle scales are taken out, and then cut into slices with a thickness of 2 to 3 mm; the slices also include the root disk and basal roots under the middle scales.

[0029] S4: Tissue Culture A slice in S3 is placed in an induction medium for tissue induction culture for 24 days, the temperature is controlled at 20°C, and the light intensity is controlled at 3500 lx. The preparation method of the induction medium in step S4 comprises the following steps: mixing MS medium, 2 mg / L 6-BA, 0.1 mg / L NAA, 30 g / L sucrose and 7 g / L agar, and then adding 1 g / L citric acid to adjust the pH to 5.8 for sterilization to obtain; S5: Obtaining regenerated plants The induced slices in S4 are placed in a growth medium, cultured for 2 months, the temperature is controlled at 20°C, and the light intensity is controlled at 3500 lx, to obtain cut lily regenerated plants, wherein the preparation method of the growth medium in S5 comprises the following steps: 1 / 2MS medium, 0.5 mg / L 6-BA, 0.3 mg / L NAA, 0.2 mg / L IBA, 30 g / L sucrose and 7 g / L agar are mixed, and then 1 g / L citric acid is added to adjust the pH to 5.8, and sterilized to obtain; S6: Recultivation of rooted bulblets The cut lily regenerated plants obtained in S5 are cultured in growth medium according to their diameters, and cultured continuously to obtain cut lily rooted bulblets of uniform size; S7: Soil fertilization Peat and perlite are mixed in a mass ratio of 3:1 to prepare a soil matrix, and decomposed organic fertilizer is added to the soil matrix, and the decomposed organic fertilizer and the soil matrix are evenly stirred, and then polyacrylamide and organic matter are added to the soil matrix to obtain a fertilized soil matrix; the organic matter comes from the native soil where the cut lily is located in step S1, and the organic fertilizer includes microorganisms, microbial products and some animals in the soil, such as earthworms, to obtain a cultivation matrix, and the addition of polyacrylamide can increase the pores in the soil matrix and improve the air permeability in the soil matrix after fertilization. In addition to increasing the pores in the soil matrix after fertilization, the addition of organic matter can also enrich the food web in the soil matrix, which is convenient for the cultivation of cut lilies. S8: Soil disinfection The soil matrix is ​​steam sterilized in a sterilization reactor. A plurality of sieve plates are arranged in the sterilization reactor. The soil matrix is ​​poured in from the uppermost sieve plate and continuously screened. During the screening, 50° C. steam is introduced to sterilize the cultivation matrix. By continuously screening the cultivation matrix, the cultivation matrix is ​​in a dispersed state, increasing the contact area between the cultivation matrix and the steam, thereby improving the sterilization effect of the cultivation matrix. Enter T1; T1: The culture medium obtained in S8 is divided into culture bottles, and the culture medium occupies 2 / 5 of the space of the culture bottle, and the culture bottle is made of transparent material; T2: Slowly add 10wt% sterile water of the culture medium inside the culture bottle into the culture bottle along the wall of the culture bottle, and let it stand for 15 minutes; T3: The cut lily regenerated plants in S5 were cultivated in the cultivation medium of the culture bottle, the temperature was controlled at 20°C, the light intensity was simulated to simulate the real light intensity, and the cultivation was carried out for 3 days; T4: The cut lily regenerated plants cultured in T3 were taken out, and the cultivation substrate in S8 was spread at a set position without compaction during the spreading process. The obtained cultured cut lily regenerated plants were then evenly cultivated on the cultivation substrate at set intervals. Temperature control, watering, topdressing and shading management were performed in the later stage. The survival rate of the cut lilies reached 83.1%, which was significantly improved compared with the survival rate of the cut lilies in Example 1.

[0030] The invention simulates the most suitable growth conditions of cut lilies to perform transplanting and culturing on regenerated plants of cut lilies, so that the regenerated plants of cut lilies grow more roots and improve the survival rate of the regenerated plants of cut lilies during cultivation.

[0031] Example 3 A high-quality and efficient culture method for cut lily bulblets comprises the following steps: S1: Sterilization Select the bulbs of cut lilies without disease spots and mechanical damage on the surface, place them in a 1000-fold diluted fungicide, soak for 20 minutes, take them out, and wash them with running water; S2: Pre-culture The cut lily bulbs treated in S1 are transferred to a preculture medium and precultured for 3 months. The preparation method of the preculture medium in step S2 comprises the following steps: mixing MS culture medium, 80 g / L sucrose and 7 g / L agar, adding 1 g / L citric acid to adjust the pH to 5.8, and sterilizing to obtain a preculture medium that is a high-sugar hypertonic culture medium, which can reduce the water content inside the cut lily bulbs and avoid inactivation of the cut lily bulbs; S3: Slice The outer scales of the cut lily bulbs pre-cultured in S2 are removed, the middle scales are taken out, and then cut into slices with a thickness of 2 to 3 mm; the slices also include the root disk and basal roots under the middle scales.

[0032] S4: Tissue Culture A slice in S3 is placed in an induction medium for tissue induction culture for 24 days, the temperature is controlled at 20°C, and the light intensity is controlled at 3500 lx. The preparation method of the induction medium in step S4 comprises the following steps: mixing MS medium, 2 mg / L 6-BA, 0.1 mg / L NAA, 30 g / L sucrose and 7 g / L agar, adding 1 g / L citric acid to adjust the pH to 5.8, and sterilizing to obtain; S5: Obtaining regenerated plants The induced slices in S4 are placed in a growth medium, cultured for 2 months, the temperature is controlled at 20°C, and the light intensity is controlled at 3500 lx, to obtain cut lily regenerated plants, wherein the preparation method of the growth medium in S5 comprises the following steps: 1 / 2MS medium, 0.5 mg / L 6-BA, 0.3 mg / L NAA, 0.2 mg / L IBA, 30 g / L sucrose and 7 g / L agar are mixed, and then 1 g / L citric acid is added to adjust the pH to 5.8, and sterilized to obtain; S6: Recultivation of rooted bulblets The cut lily regenerated plants obtained in S5 are cultured in growth medium according to their diameters, and cultured continuously to obtain cut lily rooted bulblets of uniform size; S7: Soil fertilization Peat and perlite are mixed in a mass ratio of 3:1 to prepare a soil matrix, decomposed organic fertilizer is added to the soil matrix, and the decomposed organic fertilizer and the soil matrix are evenly stirred, and polyacrylamide and organic matter are added to the soil matrix to obtain a fertilized soil matrix; the organic matter comes from the native soil where the cut lily is located in step S1, and the organic matter includes microorganisms, microbial products and some animals in the soil, such as earthworms, to obtain a cultivation matrix, and the addition of polyacrylamide can increase the pores in the soil matrix and improve the air permeability in the soil matrix after fertilization. In addition to increasing the pores in the soil matrix after fertilization, the addition of organic matter can also enrich the food web in the soil matrix, which is convenient for the cultivation of cut lilies. S8: Soil disinfection The soil matrix is ​​steam sterilized in a sterilization reactor. A plurality of sieve plates are arranged in the sterilization reactor. The soil matrix is ​​poured in from the uppermost sieve plate and continuously screened. During the screening, 50° C. steam is introduced to sterilize the cultivation matrix. By continuously screening the cultivation matrix, the cultivation matrix is ​​in a dispersed state, increasing the contact area between the cultivation matrix and the steam, thereby improving the sterilization effect of the cultivation matrix. Enter T1; T1: The culture medium obtained in S8 is divided into culture bottles, and the culture medium occupies 2 / 5 of the space of the culture bottle, and the culture bottle is made of transparent material; T2: Slowly add 10wt% sterile water of the culture medium inside the culture bottle into the culture bottle along the wall of the culture bottle, and let it stand for 15 minutes; T3: The cut lily regenerated plants in S5 were cultivated in the cultivation medium of the culture bottle, the temperature was controlled at 20°C, the light intensity was simulated to simulate the real light intensity, and the cultivation was carried out for 3 days; The temperature is controlled to decrease continuously within one day according to a temperature reduction curve, and the cut lily regenerated plants are cultured for one day, wherein the temperature reduction curve is a smooth decreasing curve with time as an independent variable and the temperature ranges from 15° C. to 0° C.; the temperature is controlled to increase continuously within one day according to a temperature increase curve, and the cut lily regenerated plants are cultured for one day, wherein the temperature increase curve is a smooth increasing curve with time as an independent variable and the temperature ranges from 15° C. to 32° C.; during this period, the dead cut lily regenerated plants are removed; The present invention simulates extreme temperature conditions during the transplanting and culturing of cut lily regenerated plants, exercises the cut lily regenerated plants, and eliminates dead cut lily regenerated plants, thereby avoiding the waste of manpower and material costs due to the death of the cut lily regenerated plants during actual cultivation.

[0033] T4: Take out the cut lily regenerated plants cultured in T3, spread the cultivation medium in S8 at the set position without compacting, and then evenly cultivate the obtained cultured cut lily regenerated plants on the cultivation medium at set intervals, and perform temperature control, watering, topdressing and shading management in the later stage.

[0034] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A high-quality and efficient method for cultivating cut lily bulblets, characterized in that: The steps include: S1: Sterilization Select the bulbs of cut lilies, place them in a 1000-1200 times diluted fungicide, soak for 10-30 minutes, then take them out and wash them with running water; S2: Pre-culture The cut lily bulbs treated in S1 were transferred to the preculture medium and precultured for 3-4 months; S3: Slice The outer scales of the cut lily bulbs pre-cultured in S2 are removed, the middle scales are taken out, and then cut into slices with a thickness of 2 to 3 mm; the slices also include the root disk and basal roots under the middle scales. S4: Tissue Culture Take one slice from S3 and place it in the induction medium for tissue induction culture for 16-25 days, with the temperature controlled at 20-30°C and the light intensity controlled at 3000-4000lx; S5: Obtaining regenerated plants The induced slices in S4 are placed in a growth medium and cultured for 1-2 months, with the temperature controlled at 20-30°C and the light intensity controlled at 3000-4000 lx to obtain cut lily regenerated plants; S6: Recultivation of rooted bulblets The cut lily regenerated plants obtained in S5 are cultured in growth medium according to their diameters, and cultured continuously to obtain cut lily rooted bulblets of uniform size; S7: Soil fertilization After peat and perlite are mixed in a mass ratio of 3:1 to prepare a soil matrix, polyacrylamide and decomposed organic fertilizer are added and stirred evenly to obtain a fertilized soil matrix; S8: Soil disinfection The soil substrate after fertilization in S7 is sterilized by steam to obtain a cultivation substrate. S9: Daughter sphere matrix culture Spread the cultivation substrate in S8 at the set position with a thickness of ≥5 cm. Do not compact the substrate during the spreading process. Then plant the rooted bulbs of cut lilies in S6 evenly on the substrate at set intervals, cover with 4-5 cm of soil, and perform temperature control, watering, topdressing and shading management in the later stage.

2. A high-quality and efficient culture method for cut lily bulbs according to claim 1, characterized in that: The method for preparing the preculture medium in step S2 comprises the following steps: mixing MS culture medium, 80 g / L sucrose and 7 g / L agar, adding 1 g / L citric acid to adjust the pH to 5.0-6.0, and sterilizing to obtain the preculture medium.

3. A high-quality and efficient culture method for cut lily bulbs according to claim 1, characterized in that: The method for preparing the induction medium in step S4 comprises the following steps: mixing MS medium, 2 mg / L 6-BA, 0.1 mg / L NAA, 30 g / L sucrose and 7 g / L agar, adding 1 g / L citric acid to adjust the pH to 5.0-6.0, and sterilizing to obtain the induction medium.

4. The method for cultivating high-quality and efficient cut lily bulbs according to claim 1, characterized in that: The preparation method of the growth medium in S5 comprises the following steps: mixing 1 / 2MS medium, 0.5mg / L6-BA, 0.3mg / LNAA, 0.2mg / L IBA, 30g / L sucrose and 7g / L agar, adding 1g / L citric acid to adjust the pH to 5.0-6.0, sterilizing, and obtaining the growth medium.

5. The method for cultivating high-quality and efficient cut lily bulbs according to claim 1, characterized in that: In the steam sterilization reactor of step S8, a plurality of sieve plates are arranged, and the soil matrix is ​​poured in from the uppermost sieve plate and continuously screened, during which steam at 50-60° C. is introduced to sterilize the soil matrix.

6. A high-quality and efficient culture method for cut lily bulbs according to claim 1, characterized in that: In the process of mixing the decomposed organic fertilizer and the soil matrix uniformly in step S7, polyacrylamide is added to the soil matrix, wherein the organic fertilizer comes from the original soil where the cut lily is located in step S1.

7. A high-quality and efficient culture method for cut lily bulbs according to claim 1, characterized in that: It also includes the transplanting of cut lily regeneration plants, the specific steps are as follows: T1: The culture medium obtained in S8 is divided into culture bottles, and the culture medium occupies 2 / 5 of the space of the culture bottle, and the culture bottle is made of transparent material; T2: Slowly add 10wt% sterile water of the culture medium inside the culture bottle into the culture bottle along the wall of the culture bottle, and then let it stand for 10-15 minutes; T3: The cut lily regenerated plants in S5 were cultivated in the cultivation medium of the culture bottle, the temperature was controlled at 18-22°C, the light intensity was simulated to the real light intensity, and the cultivation was carried out for 2-3 days; T4: Take out the cut lily regenerated plants cultured in T3, spread the cultivation medium in S8 at the set position without compacting, and then evenly cultivate the obtained cultured cut lily regenerated plants on the cultivation medium at set intervals, and perform temperature control, watering, topdressing and shading management in the later stage.

8. A high-quality and efficient culture method for cut lily bulbs according to claim 7, characterized in that: The method also includes training of cut lily regenerated plants, which specifically includes the following steps: after the cut lily regenerated plants are transplanted and cultured for 2-3 days at T3, the temperature is controlled to be continuously reduced within one day according to a temperature reduction curve, and the cut lily regenerated plants are cultured for one day, wherein the temperature reduction curve uses time as an independent variable, and the temperature smoothly decreases from 12-18°C to 0-3°C; then the temperature is controlled to be continuously increased within one day according to a temperature increase curve, and the cut lily regenerated plants are continued to be cultured for one day, wherein the temperature increase curve uses time as an independent variable, and the temperature smoothly increases from 12-18°C to 30-37°C; and dead cut lily regenerated plants are removed during the training.