Composition for preventing and treating soybean fusarium root rot and application thereof

Abstract

The invention relates to a composition for preventing and treating soybean fusarium root rot and application of the composition, the composition comprises meconazole and prothioconazole, and the mass ratio of the meconazole to the prothioconazole is 1: 1-5: 1. The composition also comprises 2% of a surfactant 500LQ, a wetting dispersant 1% of alkyl alcohol polyoxyethylene ether W600, 2% of polyaryl ether sulfate, 2% of polyvinyl alcohol, 4% of an anti-freezing agent ethylene glycol, 0.7% of a thickener xanthan gum, 0.2% of a preservative Kathon and 0.1% of an antifoaming agent AF1501. The composition for preventing and treating soybean fusarium root rot pathogenic bacteria is applied during seed treatment, and the good prevention and treatment effect on soybean fusarium root rot is achieved; and the composition for preventing and treating the soybean fusarium root rot pathogenic bacteria is safe to soybeans, does not influence soybean seedling emergence and the like.

Description

Technical Field

[0001] The invention relates to a bactericidal composition and application thereof, in particular to a composition for preventing and treating soybean fusarium root rot and application thereof. Background Art

[0002] Soybean is an important grain and oil crop. It is not only an important source of feed protein and edible protein, but also an important raw material for vegetable oils and fats. With the changes in the international trade environment in recent years, soybean has shown an increasingly important position in the world's economic oil crops, and its yield and quality are closely related to the country's food security and agricultural development strategy. We must further promote the soybean and oilseed production capacity improvement project, consolidate the results of soybean expansion, and support the development of high-oil and high-yield varieties. However, in recent years, soybean root rot has occurred frequently in my country, causing serious impacts on soybean yield and quality, seriously hindering the urgent need to increase the supply of domestic soybeans.

[0003] Soybean root rot is a worldwide soil-borne disease caused by a combination of multiple pathogens. It is mainly caused by pathogens that survive in the soil for a long time infecting the roots and stems of soybean plants, causing serious damage and huge economic losses every year. The pathogens of soybean root rot are complex and diverse. In the field, it is usually caused by multiple different pathogens infecting the same soybean plant simultaneously or successively, resulting in discoloration and rot of the roots and stems. In 2023, my country's Ministry of Agriculture and Rural Affairs has listed it as a Class I crop disease. The pathogens reported so far mainly include Fusarium spp., Phytophthora sojae, Pythium spp., Rhizoctonia solani, Phomopsis spp., etc.

[0004] Fusarium is an important pathogenic fungus that causes soybean root rot. It mainly invades the plant's vessels and vascular tissues with mycelium and spores. The hyphae and conidiophores continue to grow and block the vessels, causing symptoms such as wilting and root rot. In addition, it also produces fungal toxins that destroy cell structure, and in severe cases, causes the death of the host plant. The main types of Fusarium that cause soybean root rot reported in the literature are: Fusarium oxysporum (F.oxysporum), Fusarium solani (F.solani), Fusarium graminearum (F.graminearum), Fusarium equiseti (F.equiseti), Fusarium brachygibbosum (F.brachygibbosum), Fusarium proliferatum (F.proliferatum), Fusarium moniliforme (F.moniliforme), Fusarium tricinctum, Fusarium verticillioides, Fusarium verticillioides, Fusarium avenaceum (F.avenaceum), Fusarium semitectum (F.semitectum), and Fusarium acuminatum (F.acuminatum).

[0005] At present, chemical control is still one of the most effective measures to prevent and control plant diseases caused by various Fusarium soybean root rot pathogens. Both cypermethrin and prothioconazole are DMI fungicides. Unlike traditional azole fungicides, cypermethrin is the first new isopropanol triazole fungicide developed by BASF. It contains chiral carbon in its molecular structure and can rotate freely. It has a flexible and changeable spatial structure, which can make the agent bind to the target efficiently and not easy to be off-target, thereby slowing down the development of bacterial resistance. The target protein of DMI fungicides is sterol 14α demethylase (CYP51). However, there are three CYP51 subunits in Fusarium, namely CYP51A, CYP51B and CYP51C. The latest research results of the research team show that clofoconazole mainly acts on the CYP51C protein of Fusarium, while prothioconazole mainly acts on the CYP51B protein. Therefore, there are differences in the binding sites of clofoconazole and prothioconazole, which are less likely to produce cross-resistance and have the potential for combination. Summary of the invention

[0006] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a composition for preventing and treating soybean Fusarium root rot and its application, which effectively solves the problems mentioned in the above background technology.

[0007] To achieve the above object, the present invention provides the following technical solution: the present invention comprises cypermethrin and prothioconazole, and the mass ratio of cypermethrin to prothioconazole is 1:1 to 5:1.

[0008] Preferably, the composition further comprises 2% surfactant, 3% wetting and dispersing agent, 4% antifreeze agent, 0.7% thickener, 0.2% preservative and 0.1% defoaming agent.

[0009] Preferably, the 2% surfactant is nonionic hydroxy polyethylene oxide block copolymer 500LQ.

[0010] Preferably, the 3% wetting and dispersing agent is composed of 1% alkyl alcohol polyoxyethylene ether W600 and 2% polyarylether sulfate.

[0011] Preferably, the 4% antifreeze agent is ethylene glycol.

[0012] Preferably, the 0.7% thickener is xanthan gum.

[0013] Preferably, the 0.2% preservative is Kathon.

[0014] Preferably, the 0.1% defoaming agent is modified organosilane defoaming agent AF1501.

[0015] The composition is used in preventing and controlling soybean fusarium root rot.

[0016] Beneficial effects: The composition for controlling soybean Fusarium root rot pathogens applied during seed treatment has a good control effect on soybean Fusarium root rot; and the composition for controlling soybean Fusarium root rot pathogens is safe to soybeans and does not affect soybean emergence. DETAILED DESCRIPTION

[0017] The specific implementation modes of the present invention are described in further detail below.

[0018] Embodiment 1, the present invention provides a composition for preventing and controlling soybean Fusarium root rot and its application, comprising cypermethrin and prothioconazole, wherein the mass ratio of cypermethrin to prothioconazole is 1:1 to 5:1.

[0019] The composition further comprises 2% surfactant, 3% wetting and dispersing agent, 4% antifreeze agent, 0.7% thickener, 0.2% preservative and 0.1% defoaming agent.

[0020] The 2% surfactant is nonionic hydroxy polyethylene oxide block copolymer 500LQ.

[0021] The 3% wetting and dispersing agent is composed of 1% alkyl alcohol polyoxyethylene ether W600 and 2% polyarylether sulfate.

[0022] The 4% antifreeze agent is ethylene glycol.

[0023] The 0.7% thickener is xanthan gum.

[0024] The 0.2% preservative is Kathon.

[0025] The 0.1% defoamer is modified organic silane defoamer AF1501.

[0026] The composition is used in preventing and controlling soybean fusarium root rot.

[0027] Sensitivity test of clofoconazole and prothioconazole against soybean Fusarium root rot pathogen;

[0028] Preparation of a composition for preventing and controlling the pathogenic bacteria of soybean fusarium root rot: using a wet grinding method, weighing a certain amount of 2% surfactant 500LQ, a wetting and dispersing agent 1% alkyl alcohol polyoxyethylene ether W600 and 2% polyarylether sulfate, 2% polyvinyl alcohol, 4% antifreeze agent ethylene glycol, 0.7% thickener xanthan gum, 0.2% preservative kason and 0.1% defoamer AF1501, adding deionized water and stirring evenly with a magnetic stirrer, adding a certain amount of The original drugs clofoconazole and prothioconazole were sheared evenly with a shearing machine, and the evenly sheared slurry was transferred to a vertical sand mill, and 1.5 times the mass of the slurry was added with zirconium oxide beads (diameter 0.6-0.8mm) and sanded at room temperature. The grinding time was about 1-2 hours, and the speed of the sand mill was 1000r / min. After the sand grinding was completed, samples were taken for testing of particle size, suspension rate and other indicators. The stable suspension with a particle size of 4.0 microns can be used as a test sample, stored and reserved.

[0029] The mycelial growth rate method was used to determine the sensitivity of clofoconazole and prothioconazole to the pathogen of soybean Fusarium root rot, as follows:

[0030] The original drug was dissolved in dimethyl sulfoxide (DMSO) and prepared as 10 4 μg / mL mother solution, store in a refrigerator at 4°C for later use. Then, according to the expected test results, set 6 to 8 concentrations in the range of 10% to 90% of the inhibitory rate of the test inhibitor on the mycelial growth of pathogens, and dilute the prepared mother solution with DMSO as the solvent. Add 45μL of the corresponding concentration of the agent or DMSO (as a control) to a 50mL centrifuge tube, then add 45mL of V8 culture medium, mix upside down, pour into the culture dish, and repeat three times in each group. Take a 5mm diameter cake on the same circumference as the edge of the colony of the cultured test strain, inoculate it with the mycelium side down in the center of the culture medium, and culture it in the dark at 25°C for 3 days. When the colony diameter is larger than 2 / 3 of the diameter of the culture dish, measure the colony diameter by the cross method and record it. Calculate the growth inhibition rate according to the following formula:

[0031]

[0032] EC of fluazifop-doxconazole and prothioconazole against the pathogen of soybean Fusarium root rot 50 The values ​​are shown in Table 1.

[0033] Table 1

[0034]

[0035] Determination of the virulence of binary complexes against the dominant pathogen of soybean Fusarium root rot;

[0036] Prepare 100 μg / mL clofoconazole and prothioconazole mother solutions with dimethyl sulfoxide, respectively. Dilute with dimethyl sulfoxide to obtain 20 μg / mL clofoconazole and prothioconazole mother solutions. Mix clofoconazole and prothioconazole solutions according to mass ratios of 0:1, 1:0, 1:1, 5:1, and 1:5, respectively, to obtain clofoconazole and prothioconazole binary compound compositions 1-5, respectively. Set 5 concentrations of 0.12 μg / mL, 0.6 μg / mL, 1.2 μg / mL, 6 μg / mL, and 12 μg / mL, respectively.

[0037] 45 μL of the binary composite composition 1-5 of clofoconazole and prothioconazole was respectively taken with a pipette and added to 45 mL of sterilized V8 medium (Campbell Soup, Company Camden, NJ, USA) cooled to 45°C to make the bactericide content 1‰. The culture medium containing the composition was poured into a culture dish with a diameter of 9 cm. The treatment with only 45 μL of dimethyl sulfoxide was added as a blank control. Each composition was repeated 3 times.

[0038] When the pathogen grows to the edge of the culture dish on the V8 plate, it is allowed to grow for another 8 hours. Old mycelium is taken along the edge of the culture dish with a puncher to prepare a bacterial cake with a diameter of 6 mm. The mycelium surface is inoculated downward into a culture medium containing the composition and a control culture medium, and the cake is cultured in a dark incubator at 25°C. The colony diameter is measured after 2 days.

[0039] The colony diameter was measured by the cross method, and the inhibition rate of the binary compound composition 1-5 of clofoconazole and prothioconazole on the mycelial growth of the test strain was calculated based on the average colony diameter. Then the inhibition rate was converted into a probability value (Y), and the concentration of the agent was converted into a logarithmic value with a base of 10 (X). A regression line was drawn in Microsoft Excel, and the toxicity regression curve equation Y=a+bX of the two single agents and their respective mixed agents against soybean Fusarium root rot pathogens, as well as the correlation coefficient r and the effective inhibition concentration EC 50 value.

[0040] According to EC 50 The combined toxicity of clofoconazole and prothioconazole was calculated using the Sun Yunpei method to evaluate the combined effect of the compound preparation, and the co-toxicity coefficient was calculated as follows:

[0041] The theoretical toxicity index TTI of the mixed preparation = ∑(toxicity index of a certain drug × mass percentage of the active ingredient of the drug in the mixed agent)

[0042]

[0043] Among them: If the co-toxicity coefficient CTC > 120, it indicates a synergistic effect; when the co-toxicity coefficient CTC ≤ 80, it is an antagonistic effect, and when 80 < CTC < 120, it is an additive effect.

[0044] The toxicities of the binary compound compositions 1 - 5 of cyflufenamid and prothioconazole against Fusarium oxysporum and Fusarium graminearum are shown in Table 2.

[0045] Table 2

[0046]

[0047] According to the inhibition rates of the single agents and the mixed agents against soybean Fusarium root rot, the combined effect of the mixed preparation was evaluated by the Sun Yunpei method.

[0048] The combined toxicity evaluations of the binary compound compositions 1 - 5 of cyflufenamid and prothioconazole against Fusarium oxysporum (F. oxysporum) and Fusarium graminearum (F. graminearum) are shown in Table 3.

[0049] Table 3

[0050]

[0051]

[0052] The binary compound compositions 3 - 5 of cyflufenamid and prothioconazole have antibacterial effects on different Fusarium fungi. Moreover, the binary compound compositions 3 and 4 of cyflufenamid and prothioconazole show synergistic effects on both Fusarium fungi. Further greenhouse efficacy tests were carried out to achieve the maximum economic benefits and synergistic effects.

[0053] Greenhouse efficacy test of the composition for controlling the pathogenic bacteria of soybean Fusarium root rot;

[0054] Based on the results in Table 3, considering the combined toxicity evaluation results, economic costs, and safety factors, a composition for controlling the pathogenic bacteria of soybean Fusarium root rot was prepared for greenhouse efficacy tests.

[0055] The greenhouse efficacy test used the composition for controlling the pathogenic bacteria of soybean Fusarium root rot and the control agents.

[0056] Control agents: 400 g / L cyflufenamid suspension concentrate, 13% prothioconazole suspension concentrate.

[0057] 2 days before sowing, mix and coat the seeds with the composition for controlling Fusarium rot of soybean or the control agent at a rate of 100 g per 100 kg of seeds, dry them in a cool place, and prepare for sowing.

[0058] The experiment was carried out in a greenhouse. The soybean variety was Heihe 43. There were 4 treatments in total, each repeated 3 times, including a blank control area, an area treated with a composition for controlling soybean Fusarium root rot pathogens, and a control agent area.

[0059] Surveys were conducted 30 days and 60 days after soybean emergence to determine the efficacy of the combination for controlling soybean Fusarium root rot pathogens and the control agent on soybean Fusarium root rot. Each treatment was surveyed, and the total number of plants, the number of diseased plants at each level, and their corresponding disease levels (0, 1, 2, 3, 4, 5) were recorded, with the highest disease level being 5; the soybean emergence rate was also recorded to evaluate the safety of the agent to the crop. The disease grading standards are shown in Table 4.

[0060] Table 4

[0061]

[0062]

[0063] Calculation method of drug efficacy:

[0064] Disease index = ∑ (number of diseased plants at each level × corresponding disease level) / (total number of plants surveyed × highest disease level) × 100

[0065] Disease index control effect (%) = (average disease index of blank control area - average disease index of treated area) / average disease index of blank control area × 100

[0066] The control effect of the agent on soybean Fusarium root rot was investigated 30 days and 60 days after emergence. The field control effect of the composition for controlling soybean Fusarium root rot pathogens on soybean Fusarium root rot is shown in Table 5.

[0067] Table 5

[0068]

[0069]

[0070] The results show that the composition for controlling soybean Fusarium root rot pathogens applied during seed treatment has a good control effect on soybean Fusarium root rot; and the composition for controlling soybean Fusarium root rot pathogens is safe to soybeans and does not affect soybean emergence.

[0071] Beneficial effects: The composition for controlling soybean Fusarium root rot pathogens applied during seed treatment has a good control effect on soybean Fusarium root rot; and the composition for controlling soybean Fusarium root rot pathogens is safe to soybeans and does not affect soybean emergence.

[0072] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composition for preventing and treating soybean fusarium root rot, characterized in that: The invention comprises fluconazole and prothioconazole, wherein the mass ratio of fluconazole to prothioconazole is 1:1 to 5:

1.

2. A composition for preventing and treating soybean Fusarium root rot according to claim 1, characterized in that: The composition further comprises 2% surfactant, 3% wetting and dispersing agent, 4% antifreeze agent, 0.7% thickener, 0.2% preservative and 0.1% defoaming agent.

3. A composition for preventing and treating soybean Fusarium root rot according to claim 2, characterized in that: The 2% surfactant is nonionic hydroxy polyethylene oxide block copolymer 500LQ.

4. A composition for preventing and treating soybean Fusarium root rot according to claim 2, characterized in that: The 3% wetting and dispersing agent is composed of 1% alkyl alcohol polyoxyethylene ether W600 and 2% polyarylether sulfate.

5. A composition for preventing and treating soybean Fusarium root rot according to claim 2, characterized in that: The 4% antifreeze agent is ethylene glycol.

6. A composition for preventing and treating soybean Fusarium root rot according to claim 2, characterized in that: The 0.7% thickener is xanthan gum.

7. A composition for preventing and treating soybean Fusarium root rot according to claim 2, characterized in that: The 0.2% preservative is Kathon.

8. A composition for preventing and treating soybean Fusarium root rot according to claim 2, characterized in that: The 0.1% defoamer is modified organic silane defoamer AF1501.

9. Use of the composition according to any one of claims 1 to 8 in preventing and controlling soybean Fusarium root rot.

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