Novel green and environment-friendly bactericidal composition and bactericide

Abstract

The invention belongs to the technical field of bactericides, and particularly relates to a novel green and environment-friendly bactericidal composition and a bactericide. The invention relates to a novel green and environment-friendly bactericidal composition. The effective components of the novel green and environment-friendly bactericidal composition are compounded by emodin monomethyl ether and pyrazolostrobin or valerianovalicarb-isopropyl, wherein the mass ratio of the emodin monomethyl ether to the tetrazolium pyrazole carbamate is (1-15): (35-1); the mass ratio of the physcion to the valerian bacteria amine is (1-20): (20-1). Emodin monomethyl ether is compounded with existing pyrazolostrobin or valerian bacteria, the activity of the compounded combination on crop disease bacteria such as wheat gray mold is measured through a mycelial growth rate method, the combined effect of the compounded combination is calculated and evaluated through a Wadley method, and it is found that when the emodin monomethyl ether is compounded with pyrazolostrobin or valerian bacteria, the activity of the compounded combination on crop disease bacteria such as wheat gray mold is remarkably improved. A synergistic interaction effect is shown, the physcion can be protected, and the utilization rate of an existing medicament is improved; in addition, the control effect on crop diseases can be improved, and accumulation and development of drug resistance of disease bacteria are delayed.

Description

Technical Field

[0001] The present invention belongs to the technical field of fungicides, and in particular relates to a novel green and environment-friendly fungicidal composition and a fungicide. Background Art

[0002] Physcion is a novel botanical fungicide jointly developed by the Institute of Plant Protection and Soil and Fertilizer of Hubei Academy of Agricultural Sciences and Inner Mongolia Qingyuanbao Biotechnology Co., Ltd. Physcion is a natural anthraquinone compound extracted from the roots and stems of Rheum officinale, a traditional Chinese medicine plant of the Polygonaceae family. It is a plant secondary metabolite. Its chemical name is: 1,8-dihydroxy-3-methoxy-6-methylanthraquinone, and its structural formula is as follows:

[0003]

[0004] Physcion can interfere with the structure and function of fungal cell membranes, causing cell membrane rupture and increased permeability, thereby preventing the balance of substances inside and outside fungal cells, and ultimately leading to fungal cell death; in addition, Physcion can also affect the absorption process of nutrients such as amino acids and sugars by fungi, restricting the growth and reproduction of fungi, and thus inhibiting the occurrence of diseases. Physcion not only has a bactericidal effect, but also can activate the active immune system of plants. It has the characteristics of low toxicity, fast degradation and low residue, and is suitable for the green production of crops.

[0005] Although physcion has the above advantages, like other fungicides, if used as a single component for a long time, it will inevitably cause the rapid accumulation and development of drug resistance in pathogenic bacteria, thereby reducing its prevention effect or even causing it to become ineffective. For those skilled in the art, compounding different fungicidal active ingredients and screening out compound combinations with synergistic effects can not only improve the prevention and control effect of diseases, but also delay the accumulation and development of drug resistance in pathogenic bacteria. However, not all compounding of different fungicidal active ingredients can achieve a synergistic effect.

[0006] There is no report on the combination of physaccharin methyl ether with tetrazolium pyrazone or valeramide. Summary of the invention

[0007] The purpose of the present invention is to provide a novel green and environmentally friendly bactericidal composition and bactericide to solve the problems existing when physcion is used as a single component.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A novel green and environmentally friendly fungicidal composition, wherein the active ingredients of the novel green and environmentally friendly fungicidal composition are compounded by physcion methyl ether and tetrazolylpyrrolidone or valeramide; wherein the mass ratio of physcion methyl ether to tetrazolylpyrrolidone is 1-15:35-1; and the mass ratio of physcion methyl ether to valeramide is 1-20:20-1.

[0010] Preferably, the mass ratio of physaccharin methyl ether to tetrazolium pyrrolidone is 1-15:10-1.

[0011] Preferably, the mass ratio of physaccharin methyl ether to valerianide is 1-10:5-1.

[0012] A bactericide, comprising the novel green and environmentally friendly bactericidal composition.

[0013] Preferably, the fungicide further comprises auxiliary ingredients permitted to be added in pesticide science.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The invention compounds physcion with existing tetrazolylpyridine or valerin, determines the activity of the compound combination against agricultural crop pathogens such as wheat gray mold by a mycelium growth rate method, and calculates and evaluates the combined effect of the compound combination by a Wadley method. The results show that when physcion and tetrazolylpyridine or valerin are compounded in a certain mass ratio, a synergistic effect is exhibited, physcion can be protected to a certain extent, and the utilization of existing agents can be improved; in addition, compared with a single fungicide, the control effect of crop diseases can be improved, and the accumulation and development of drug resistance of disease bacteria can be delayed. DETAILED DESCRIPTION

[0016] Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0017] 1. Test strains

[0018] Botrytis cinerea Pers. exFr. - collected from infected wheat plant samples, isolated and purified in the laboratory, and stored on PDA culture medium.

[0019] 2. Test reagents

[0020] 98% physcion methyl ether technical, 97% tetrazolylpyrazone technical, 98% valeramide technical

[0021] The test agent was first dissolved with dimethyl sulfoxide and then diluted with 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution. Multiple groups of proportions were set, and each single-dose stock solution and proportioned mixture were set with 5 mass concentration gradients according to the equal ratio method. The above agents were prepared and used immediately.

[0022] 3. Test methods

[0023] The mycelium growth rate method (Mu Liyi 1994) was used. The drug solution and the melted PDA medium were mixed at a volume ratio of 1:9, and poured into a sterile culture dish with a diameter of 9 cm to make a drug-containing plate, 10 mL / dish. The test strain was cultured on the PDA medium at 25±1℃ for 3 days, and a 5mm diameter bacterial cake was punched along the edge of the colony with a hole puncher. One bacterial cake was inoculated in the center of the drug-containing plate with the mycelium facing down. Each mass concentration of drug solution was repeated for 5 plates, and the treatment of 0.1% Tween-80 aqueous solution was set as a control. After covering the dish, it was placed in an incubator and cultured at 25±1℃ until the colony diameter reached about 6 cm. The colony growth diameter was measured by the cross method and the inhibition rate was calculated.

[0024]

[0025] 4. Data Analysis

[0026] DPS software was used to perform regression analysis on the logarithmic value of the drug concentration and the probability value of mycelium growth inhibition rate to calculate the EC of each treatment agent. 50 , and the synergistic effect of drug mixing was evaluated according to the Wadlley method.

[0027] SR≥1.5 indicates a synergistic effect; SR≤0.5 indicates an antagonistic effect; 0.5<SR<1.5 indicates an additive effect. The results are shown in Table 1-2.

[0028] Table 1 The combined toxicity results of physaccharin methyl ether and tetrazolium pyrazone against Botrytis cinerea

[0029]

[0030]

[0031] As shown in Table 1, in the indoor activity test of the combination of physalis and tetrazolylpyridine to inhibit the mycelial growth of wheat gray mold fungus Botrytis cinerea, when the mass ratio is 1:35-15 or 20:1, the synergistic coefficient SR of the combination is between 0.5-1.5, showing an additive effect; when the mass ratio is 1-15:10-1, the synergistic coefficient SR of the combination is greater than 1.5, showing a synergistic effect. This shows that when the physalis and tetrazolylpyridine are compounded in a mass ratio of 1-15:10-1, the control effect on crop diseases such as wheat gray mold is better than that of a single agent.

[0032] Table 2 The combined toxicity results of physaccharin methyl ether and valerin to Botrytis cinerea

[0033]

[0034] As shown in Table 2, in the indoor activity test of the combination of physaccharin methyl ether and valerin to inhibit the growth of mycelium of wheat gray mold fungus Botrytis cinerea, when the mass ratio is 1:20-10 or 20:1, the synergistic coefficient SR of the combination is between 0.5-1.5, showing an additive effect; when the mass ratio is 1-10:5-1, the synergistic coefficient SR of the combination is greater than 1.5, showing a synergistic effect. This shows that when the present invention combines physaccharin methyl ether and valerin at a mass ratio of 1-10:5-1, the control effect on crop diseases such as wheat gray mold is better than that of a single agent.

[0035] In summary, when physaccharin methyl ether is compounded with tetrazolium pyraclostrobin or valeramide in a certain mass ratio, it shows a synergistic effect, which can protect physaccharin methyl ether to a certain extent and improve the utilization of existing agents; in addition, compared with a single fungicide, it can improve the prevention and control effect of crop diseases and delay the accumulation and development of drug resistance of pathogenic bacteria.

Claims

1. A new type of green and environmentally friendly bactericidal composition, characterized in that: The active ingredients of the novel green and environmentally friendly fungicide composition are compounded with physaccharin methyl ether and tetrazolylpyram or valeramide; wherein, The mass ratio of physaccharin methyl ether to tetrazolium pyrrolidone is 1-15:35-1; The mass ratio of physaccharin methyl ether to valerianide is 1-20:20-1.

2. The novel green and environmentally friendly bactericidal composition according to claim 1, characterized in that: The mass ratio of physaccharin methyl ether to tetrazolium pyrrolidone is 1-15:10-1.

3. The novel green and environmentally friendly bactericidal composition according to claim 1, characterized in that: The mass ratio of physaccharin methyl ether to valerian is 1-10:5-1.

4. A bactericide, characterized in that: The bactericide comprises the novel green and environmentally friendly bactericidal composition according to any one of claims 1 to 3.

5. The bactericide according to claim 4, characterized in that The fungicide also includes auxiliary ingredients allowed to be added in pesticides.

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