Enzyme composition for oil stain treatment and preparation method thereof

Abstract

The invention relates to the technical field of household cleaning products, in particular to an enzyme composition for oil stain treatment and a preparation method of the enzyme composition. The invention relates to an enzyme composition for oil stain treatment. The enzyme composition comprises microbial fermentation liquor, a compound enzyme preparation, an anti-oil stain film-forming agent, a stabilizer, friction particles and a solvent, the strain of the microbial fermentation liquor is one or more of bacillus subtilis, pseudomonas aeruginosa and lactobacillus, and the fermentation medium of the microbial fermentation liquor contains one or more of inulin, olive oil or glucose; the biological fermentation liquid is fermented to obtain the surfactant; the compound enzyme preparation is a mixture of protease, lipase and beta-(1, 3-1, 4)-glucanase. The enzyme composition for oil stain treatment is mild, small in irritation and remarkable in effect when being used as a kitchen oil stain cleaning agent.

Description

Technical Field

[0001] The present application relates to the technical field of household cleaning products, and more specifically, to an enzyme composition for treating oil stains and a preparation method thereof. Background Art

[0002] As China's economy develops and the quality of life improves, families pay more and more attention to the hygiene and cleanliness of the kitchen. Influenced by traditional Chinese diet and cooking habits, the kitchen is full of fumes, which makes the oil stains on the range hood, gas stove and wall very sticky and has strong adhesion, which leads to heavy cleaning work.

[0003] The main components of kitchen grease are the following: dust and other tiny particles in the environment combine with evaporated oil smoke, quickly settle and adsorb on the surface of objects; grease splashes and vaporizes during frying, steaming and cooking, and then adheres to the surface of objects; unsaturated oil undergoes oxidative polymerization during the cooking process to form highly viscous thick oil, which is then oxidized and dried on the surface of objects.

[0004] At present, the cleaning agent formula for this type of kitchen oil stains is generally composed of organic solvents, surfactants and alkaline additives. However, this type of cleaning agent has a strong odor and is even toxic. If used for a long time, it is harmful to human health. At the same time, alkaline substances can also damage the skin surface. Therefore, a mild, safe and efficient kitchen oil stain cleaning agent is urgently needed. Summary of the invention

[0005] In order to improve the defects of conventional kitchen oil stain cleaners that are highly irritating and harmful to human health, the present application provides an enzyme composition for oil stain treatment and a preparation method thereof.

[0006] In the first aspect, the present application provides an enzyme composition for treating oil pollution, which adopts the following technical scheme:

[0007] An enzyme composition for treating oil pollution, comprising microbial fermentation liquid, composite enzyme preparation, anti-oil pollution film-forming agent, stabilizer, friction particles and solvent;

[0008] The bacterial species of the microbial fermentation liquid is one or more of Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus, and the fermentation medium of the microbial fermentation liquid contains one or more of inulin, olive oil or glucose; the biological fermentation liquid is fermented to obtain a biological surfactant;

[0009] The composite enzyme preparation is a mixture of protease, lipase and beta-(1,3-1,4)-glucanase.

[0010] Biosurfactants are substances that have surfactant functions when certain substances are converted into substances in microorganisms. Similar to artificially synthesized surfactants, biosurfactants can also reduce surface tension and change the wettability of the kitchen grease interface. At the same time, they can also change the microbial environment in kitchen grease and provide nutrients that aid biodegradation. Therefore, they are relatively superior to chemical surfactants.

[0011] Biological enzymes are similar to biological surfactants and are also produced by microorganisms. However, biological enzymes cannot change interfacial tension. However, they can degrade organic matter or polymers in kitchen grease, break up their polymer skeletons, reduce viscosity, and have a demulsifying effect, so that oil and water are no longer miscible, restoring their original contact glue and promoting the separation of water, oil and dust in kitchen grease.

[0012] In summary, when biosurfactants and bioenzymes are used at the same time, they will respectively play the role of demulsification and dispersion, thereby effectively improving the treatment effect of kitchen grease. At the same time, biosurfactants and bioenzymes are friendly to the human body and non-irritating, and are relatively gentler and safer.

[0013] However, since the environments required for microorganisms to produce biosurfactants and bioenzymes are different, the present application selects to add microbial fermentation broth and compound enzyme preparations, wherein the microbial fermentation broth is used to produce biosurfactants. In addition, the additionally added compound enzyme preparation can also improve the living environment of microorganisms in kitchen grease, thereby expanding the population of microorganisms, promoting the formation of more biosurfactants in the microbial fermentation broth, playing a certain positive correlation role, and promoting the enzyme composition for grease treatment to treat kitchen grease more efficiently.

[0014] There are many bacteria species that can produce biosurfactants, such as Bacillus subtilis, Pseudomonas aeruginosa, Lactobacillus acidophilus and Saccharomyces cerevisiae. However, compared with Saccharomyces cerevisiae, the biosurfactants of Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus are more effective.

[0015] Due to its special composition, kitchen grease has certain requirements for the selection of biological enzymes. Specifically, the main components of kitchen grease include fat, protein particles and sugars. Therefore, the biological enzyme will directly select a mixture of protease, lipase and glucanase. Among them, when the glucanase is β-(1,3-1,4)-glucanase, the enzyme composition for grease treatment has a particularly outstanding cleaning effect on kitchen grease.

[0016] The use of friction particles is to provide cleaning force points when cleaning kitchen grease, thereby further enhancing the demulsification effect of the biological enzyme and effectively improving the kitchen grease cleaning effect of the biological enzyme.

[0017] Preferably, the fermentation medium of the microbial fermentation broth comprises the following raw materials in concentrations: 0.02-0.08 parts of zinc chloride, 0.01-0.03 parts of calcium chloride, 0.5-1.5 parts of potassium hydrogen phosphate, 0.2-0.7 parts of potassium dihydrogen phosphate, 0.5-1.5 parts of sodium chloride, 0.1-0.5 parts of calcium bicarbonate and 5-15 parts of inulin.

[0018] The fermentation medium of the microbial fermentation broth largely determines the production of biosurfactants. As for energy materials, inulin, olive oil or glucose can be selected according to actual conditions. Inulin has a lower cost in addition to a higher biosurfactant production, so inulin is better.

[0019] Preferably, the bacterial species of the microbial fermentation liquid is a mixture of Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus, and the inoculation amount of Bacillus subtilis is 2-4%, the inoculation amount of Pseudomonas aeruginosa is 0.5-1%, and the inoculation amount of Lactobacillus acidophilus is 1-3%.

[0020] Although Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus all have relatively good biosurfactant production effects, when the three are used in combination and the above-mentioned inoculation amount is adopted at the same time, the microbial fermentation liquid will produce a larger amount of biosurfactant, and ultimately the enzyme composition for grease treatment will have a better cleaning effect on kitchen grease.

[0021] Preferably, the method for preparing the microbial fermentation broth comprises the following steps:

[0022] S1. First, inoculate Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus into Gao's medium respectively, at 30-35℃, 150-180r / min, activate and culture for 18-24h to obtain bacterial seed solution with a bacterial content of 10 7 -10 9 cfu / ml;

[0023] S2. First, inoculate the bacterial seed liquid into the fermentation medium, culture at 25-35°C, 150-180r / min, and culture for 36-48h; then add 5% glucose by total weight, 25-35°C, 150-180r / min, and further culture for 18-24h, and concentrate to obtain the microbial fermentation liquid.

[0024] Preferably, the mass ratio of the protease, lipase and β-(1,3-1,4)-glucanase is (3-5):(3-5):(2-4).

[0025] When the protease, lipase and β-(1,3-1,4)-glucanase are used in the above mass ratio, the composite enzyme preparation will have a better demulsification effect and environmental improvement effect, and ultimately the enzyme composition for oil stain treatment will have a better cleaning effect on kitchen oil stains.

[0026] Preferably, the preparation method of the anti-oil film-forming agent comprises the following steps:

[0027] Step 1: First, place 10-30 ml of ethanol in a three-necked flask and heat it to 60-80° C. under a nitrogen atmosphere. After the temperature is constant, first add 0.5-1.5 ml of azobisisobutyronitrile-tetrahydrofuran solution, then add 1.0-1.4 g of NPCEMA, 3-5 g of MPC and 7-9 ml of azobisisobutyronitrile-tetrahydrofuran solution to 120-140 ml of anhydrous ethanol, and slowly dropwise add to the three-necked flask. After the dropwise addition is complete, add 0.5-1.5 ml of azobisisobutyronitrile-tetrahydrofuran solution again, stir for 5-7 hours, stop passing nitrogen, stir again for 15-20 hours, and finally rotary evaporate and dialyze to obtain a phosphorylcholine-containing polymer;

[0028] Wherein, the concentration of azobisisobutyronitrile-tetrahydrofuran solution is 10.4 g / L;

[0029] Step 2: Add 1-3g of phosphorylcholine-containing polymer to a three-necked round-bottom flask, add 40-60ml of ethanol, introduce nitrogen, heat to 50-70°C, add 10-30ml of dopamine hydrochloride-ethanol solution, the concentration of dopamine hydrochloride-ethanol solution is 25g / L, add triethylamine under nitrogen protection until the solution is neutral, react for 10-20h, adjust the pH value to 4 with hydrochloric acid, dialyze for 60-80h, and obtain an anti-oil film-forming agent.

[0030] At present, both chemical kitchen grease cleaners and biological kitchen grease cleaners attach great importance to the initial cleaning of grease, so the cleaning effects of both are extremely outstanding, but both pay little attention to the subsequent prevention of grease after the initial cleaning, which leads to the need for people to repeatedly clean range hoods, gas stoves, and walls.

[0031] In the present application, an anti-oil film-forming agent is additionally added to the enzyme composition for oil stain treatment. After the enzyme composition for oil stain treatment cleans kitchen oil stains, a hydration film containing phosphorylcholine groups will be formed at the cleaning location. Since the phosphorylcholine groups have good anti-adhesion and anti-pollution properties, the presence of the hydration film can effectively prevent the re-formation of kitchen oil stains and reduce the possibility of repeated cleaning.

[0032] Preferably, the stabilizer is sucrose ester, the solvent is N,N-dimethylformamide, and the friction particles are colored soft beads.

[0033] Preferably, the enzyme composition for treating oil pollution comprises the following raw materials in parts by weight: 10-20 parts of microbial fermentation broth, 5-7 parts of complex enzyme preparation, 3-6 parts of anti-oil pollution film-forming agent, 1-3 parts of stabilizer, 6-8 parts of friction particles and 10-12 parts of solvent.

[0034] In a second aspect, the present application provides a method for preparing an enzyme composition for oil pollution treatment, using the following technical solution:

[0035] A method for preparing an enzyme composition for treating oil pollution, comprising the following steps: firstly dissolving 10-20 parts of microbial fermentation liquid and 5-7 parts of a composite enzyme preparation in 100 parts of deionized water; dissolving 3-6 parts in 10-12 parts of a solvent; then mixing the two solutions and adding a stabilizer and friction particles in sequence, and finally adding 0.5-1.5 parts of an emulsifier, emulsifying and mixing evenly to obtain the enzyme composition for treating oil pollution.

[0036] In summary, this application has the following beneficial effects:

[0037] 1. When biosurfactants and bioenzymes are used at the same time, they will respectively play the role of demulsification and dispersion, thereby effectively improving the treatment effect of kitchen grease. At the same time, biosurfactants and bioenzymes are friendly to the human body and non-irritating, and are relatively milder and safer. At the same time, since the environments required for microorganisms to produce biosurfactants and bioenzymes are different, the addition of microbial fermentation broth and complex enzyme preparations can make the enzyme composition for grease treatment more effective in cleaning kitchen grease.

[0038] 2. Due to its special composition, kitchen grease has certain requirements for the selection of biological enzymes. Specifically, the main components of kitchen grease include fat, protein particles and sugars. Therefore, the rotation of biological enzymes will directly select a mixture of protease, lipase and glucose enzyme. Among them, when β-(1,3-1,4)-glucanase is selected as the glucose enzyme, the enzyme composition for grease treatment has a particularly outstanding cleaning effect on kitchen grease.

[0039] 3. Although Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus all have relatively good biosurfactant production effects, when the three are used in combination and the above-mentioned inoculation amount is adopted at the same time, the microbial fermentation liquid will produce a larger amount of biosurfactant, and ultimately the enzyme composition for oil stain treatment will have a better cleaning effect on kitchen oil stains.

[0040] 4. In the present application, an anti-oil film-forming agent is additionally added to the enzyme composition for oil stain treatment. After the enzyme composition for oil stain treatment cleans kitchen oil stains, a hydration film containing phosphorylcholine groups will be formed at the cleaning location. Since the phosphorylcholine groups have good anti-adhesion and anti-pollution properties, the presence of the hydration film can effectively prevent the re-formation of kitchen oil stains and reduce the possibility of repeated cleaning. DETAILED DESCRIPTION

[0041] The present application is further described in detail below in conjunction with Examples 1-10 and Comparative Examples 1-6.

[0042] raw material

[0043] Zinc chloride, calcium chloride, potassium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, calcium hydrogen carbonate, ethanol, azobisisobutyronitrile, tetrahydrofuran and N,N-dimethylformamide are conventionally commercially available;

[0044] Bacillus subtilis CMCC63501; Pseudomonas aeruginosa CMCC10104; Lactobacillus acidophilus ATCC 4356; Saccharomyces cerevisiae ATCC26785; Protease CAS: 9001-92-7; Lipase CAS: 9001-62-1; β-(1,3-1,4)-glucanase CAS: 62213-14-3; Glucose enzyme CAS: 9032-08-0; Olive oil CAS: 8001-25-0; Glucose CAS: 50-99-7; Inulin CAS: 9005-80-5; Dopamine hydrochloride CAS: 62-31-7; Sucrose ester CAS: 25168-73-4; NPCEMA and MPC refer to CN104610516A; Emulsifier-Tween-80.

[0045] Example

[0046] Example 1

[0047] An enzyme composition for treating oil pollution comprises the following raw materials: 15g of microbial fermentation liquid, 6g of composite enzyme preparation, 4.5g of anti-oil pollution film-forming agent, 2g of stabilizer-sucrose ester, 7g of colored soft beads and 11g of N,N-dimethylformamide.

[0048] The fermentation medium of the microbial fermentation liquid includes the following raw materials in the following concentrations: 0.05 g / L zinc chloride, 0.02 g / L calcium chloride, 1.0 g / L potassium hydrogen phosphate, 0.5 g / L potassium dihydrogen phosphate, 1.0 g / L sodium chloride, 0.3 g / L calcium hydrogen carbonate and 10 g / L inulin, and the solvent is water;

[0049] The strains of the microbial fermentation liquid are a mixture of Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus, and the inoculation amount of Bacillus subtilis is 3% of the mass of the culture medium, the inoculation amount of Pseudomonas aeruginosa is 0.8% of the mass of the culture medium, and the inoculation amount of Lactobacillus acidophilus is 2% of the mass of the culture medium.

[0050] The method for preparing the microbial fermentation broth comprises the following steps:

[0051] S1. First, Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus were inoculated into Gao's medium respectively, and activated and cultured for 18 hours at 30°C and 150r / min to obtain bacterial seed liquid with a bacterial content of 10 7 cfu / ml;

[0052] S2. First, the bacterial seed solution is inoculated into the fermentation medium, and the culture temperature is 32°C, 180r / min, and the culture is carried out for 48h; then, 5% glucose by total weight is added, and the culture is carried out at 32°C, 150r / min, and the culture is further carried out for 24h, and the microbial fermentation liquid is concentrated.

[0053] The composite enzyme preparation is a mixture of protease, lipase and β-(1,3-1,4)-glucanase, and the mass ratio of the protease, lipase and β-(1,3-1,4)-glucanase is 4:4:3.

[0054] The preparation method of the anti-oil film-forming agent comprises the following steps:

[0055] Step 1: First, place 20 ml of ethanol in a three-necked flask and heat it to 70 ° C under a nitrogen atmosphere. After the temperature is constant, first add 1 ml of azobisisobutyronitrile-tetrahydrofuran solution, then add 1.2 g of NPCEMA, 4 g of MPC and 8 ml of azobisisobutyronitrile-tetrahydrofuran solution to 130 ml of anhydrous ethanol, and slowly add it dropwise to the three-necked flask. After the addition is complete, add 1 ml of azobisisobutyronitrile-tetrahydrofuran solution again, stir for 6 hours, stop passing nitrogen, stir again for 18 hours, and finally rotary evaporate and dialyze to obtain a phosphorylcholine-containing polymer;

[0056] Wherein, the concentration of azobisisobutyronitrile-tetrahydrofuran solution is 10.4 g / L;

[0057] Step 2: Add 2 g of phosphorylcholine-containing polymer to a three-necked round-bottom flask, add 50 ml of ethanol, introduce nitrogen, heat to 60°C, add 20 ml of dopamine hydrochloride-ethanol solution, the concentration of dopamine hydrochloride-ethanol solution is 25 g / L, add triethylamine under nitrogen protection until the solution is neutral, react for 12 h, adjust the pH value to 4 with hydrochloric acid, dialyze for 72 h, and obtain an anti-oil film-forming agent.

[0058] The preparation method of the enzyme composition for oil pollution treatment is as follows: first, 15g of microbial fermentation liquid and 6g of complex enzyme preparation are dissolved in 100g of deionized water; 4.5g is dissolved in 11g of solvent; then the two solutions are mixed and 2g of stabilizer-sucrose ester and 7g of friction particles-colored soft beads are added in sequence, and finally 1g of Tween-80 is added, emulsified and mixed evenly to obtain the enzyme composition for oil pollution treatment.

[0059] Example 2-3

[0060] The difference from Example 1 is that the addition amount of each component of the enzyme composition for oil pollution treatment is different, as shown in Table 1.

[0061] Table 1 Addition amount of each component of the enzyme composition for oil pollution treatment in Examples 1-3

[0062] Example 1 Example 2 Example 3 Microbial fermentation broth 15 10 20 Complex enzyme preparations 6 7 5 Anti-oil film-forming agent 4.5 6 3 Sucrose esters 2 3 1 Colored soft beads 7 6 8 N,N-Dimethylformamide 11 11 12

[0063] Example 4

[0064] The difference from Example 1 is that the strain of the microbial fermentation liquid is only Bacillus subtilis, and the inoculation amount is 6% of the mass of the culture medium.

[0065] Example 5

[0066] The difference from Example 1 is that the bacterial species in the microbial fermentation broth is only Pseudomonas aeruginosa, and the inoculation amount is 6% of the mass of the culture medium.

[0067] Example 6

[0068] The difference from Example 1 is that the strain of the microbial fermentation liquid is only Lactobacillus acidophilus, and the inoculation amount is 6% of the mass of the culture medium.

[0069] Embodiment 7-8

[0070] The difference from Example 1 is that the inoculation amount of each bacterial species in the microbial fermentation broth is different, as shown in Table 2.

[0071] Table 2 Inoculation amount of each strain of the microbial fermentation liquid of Example 1, Example 7-8

[0072] Example 1 Example 7 Example 8 Bacillus subtilis 3 2 4 Pseudomonas aeruginosa 0.8 1 0.5 Lactobacillus acidophilus 2 3 1

[0073] Examples 9-10

[0074] The difference from Example 1 is that the mass ratios of the components of the complex enzyme preparation are different, as shown in Table 3.

[0075] Table 3 Mass ratio of each component of the composite enzyme preparation in Example 1 and Examples 9-10

[0076]

[0077] Comparative Example

[0078] Comparative Example 1

[0079] A kitchen grease cleaner, which is Mr. Muscle grease cleaner.

[0080] Comparative Example 2

[0081] The difference from Example 1 is that no microbial fermentation liquid is added.

[0082] Comparative Example 3

[0083] The difference from Example 1 is that the strain of the microbial fermentation liquid is only Saccharomyces cerevisiae, and the inoculation amount is 6% of the mass of the culture medium.

[0084] Comparative Example 4

[0085] The difference from Example 1 is that no complex enzyme preparation is added.

[0086] Comparative Example 5

[0087] The difference from Example 1 is that in the composite enzyme preparation, β-(1,3-1,4)-glucanase is replaced with the same amount of glucurase.

[0088] Comparative Example 6

[0089] The difference from Example 1 is that no anti-oil film-forming agent is added.

[0090] Performance testing

[0091] Detection Methods

[0092] 1. Kitchen oil cleaning effect test

[0093] Take several tiles from the kitchen area with heavy oil pollution, cut the tiles into several pieces, weigh all the cut samples and record them as M0; then take out three samples from Examples 1-10 and Comparative Examples 1-5 respectively, attach all the samples to different cut samples, then rub them at the same frequency for 10s, rinse and dry them, weigh the cut samples again and record them as M1, finally calculate the cleaning amount and take the average value, cleaning amount = M0-M1.

[0094] The test data are shown in Table 4.

[0095] Table 4 Cleaning performance of Examples 1-10 and Comparative Examples 1-5

[0096]

[0097]

[0098] 2. Oil pollution prevention performance test

[0099] Three samples were taken from Example 1 and Comparative Example 6 respectively, and then the tile samples were washed with the samples. After drying, the samples were weighed and recorded as m0. The tiles were then hung vertically and olive oil was sprayed on the tile mask. After standing for 24 hours, the tiles were weighed again and recorded as m1. Finally, the adhesion amount was calculated and the average value was taken, adhesion amount = m1-m0.

[0100] The test data are shown in Table 5.

[0101] Table 5 Oil pollution prevention performance test table of Example 1 and Comparative Example 6

[0102] Adhesion amount / g Adhesion amount / g Example 1 0.72 Comparative Example 6 5.83

[0103] Referring to Example 1 and Comparative Example 1 and in combination with Table 4, it can be seen that the cleaning amount of Example 1 is not much different from that of Comparative Example 1, which shows that the enzyme composition for oil stain treatment of the present application also has an extremely good kitchen oil stain treatment effect, which can effectively replace conventional chemical kitchen oil stain cleaners and is also more gentle and safe.

[0104] Referring to Example 1, Comparative Example 2 and Comparative Example 4 and combining with Table 4, it can be seen that compared with Example 1, the cleaning amount of Comparative Example 2 and Comparative Example 4 is significantly reduced, which shows that the microbial fermentation liquid and the complex enzyme preparation have a significant kitchen oil cleaning effect.

[0105] The reason is that biosurfactants can also reduce surface tension and change the wettability of the kitchen oil interface. At the same time, they can also change the microbial environment in kitchen oil and provide nutrients that help biodegradation.

[0106] Biological enzymes can degrade organic matter or polymers in kitchen grease, break up their polymer skeletons, reduce viscosity, and achieve a demulsification effect, making oil and water no longer miscible, restoring their original contact glue, and promoting the separation of water, oil, and dust in kitchen grease.

[0107] In addition, the additionally added complex enzyme preparation can also improve the living environment of microorganisms in kitchen grease, thereby expanding the microbial population and promoting the formation of more biosurfactants in the microbial fermentation liquid, playing a certain positive correlation role, and promoting the enzyme composition for grease treatment to treat kitchen grease more efficiently.

[0108] Referring to Examples 1-3 and in combination with Table 4, it can be seen that the cleaning amounts of Examples 2-3 are slightly reduced compared to Example 1, which indicates that when the components of the enzyme composition for treating oily dirt adopt the proportions of Example 1, the prepared enzyme composition for treating oily dirt will have a better kitchen oily dirt cleaning effect.

[0109] With reference to Example 1, Examples 4-6 and Comparative Example 3 and in combination with Table 4, it can be seen that, relative to Example 1, the cleaning amounts of Examples 4-6 and Comparative Example 3 are significantly reduced, among which the cleaning amount of Comparative Example 3 is the lowest. This shows that there are many species of bacteria that can produce biosurfactants, such as Bacillus subtilis, Pseudomonas aeruginosa, Lactobacillus acidophilus and Saccharomyces cerevisiae, but relative to Saccharomyces cerevisiae, the effect of producing biosurfactants by Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus is more prominent.

[0110] At the same time, compared with using Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus alone, using the three together can make the enzyme composition for treating grease have a better kitchen grease treatment effect.

[0111] Referring to Example 1 and Examples 7-8 and in combination with Table 4, it can be seen that the cleaning amount of Examples 7-8 is slightly reduced compared with Example 1, which shows that when Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus adopt the mass ratio of Example 1, the prepared enzyme composition for oil stain treatment will have a better kitchen oil stain cleaning effect.

[0112] Referring to Example 1 and Examples 9-10 and in combination with Table 4, it can be seen that the cleaning amount of Examples 9-10 is significantly reduced compared with that of Example 1. This shows that when the components of the composite enzyme preparation adopt the mass ratio of Example 1, the prepared enzyme composition for oil stain treatment will have a better kitchen oil stain cleaning effect.

[0113] Referring to Example 1 and Comparative Example 5 and combining with Table 4, it can be seen that the cleaning amount of Comparative Example 5 is significantly reduced compared with Example 1, which shows that, compared with glucose enzyme, β-(1,3-1,4)-glucanase has a better kitchen oil cleaning effect.

[0114] With reference to Example 1 and Comparative Example 6 and in combination with Table 5, it can be seen that, relative to Example 1, the adhesion amount of Comparative Example 6 is significantly improved, which indicates that the anti-oil film-forming agent can effectively prevent subsequent oil stains.

[0115] The reason is that after the enzyme composition for grease treatment is used to clean kitchen grease, a hydration film containing phosphorylcholine groups will be formed at the cleaning location. Since phosphorylcholine groups have good anti-adhesion and anti-pollution properties, the presence of the hydration film can effectively prevent the re-formation of kitchen grease and reduce the possibility of repeated cleaning.

[0116] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. An enzyme composition for oil pollution treatment, characterized in that: Including microbial fermentation liquid, complex enzyme preparation, anti-oil film-forming agent, stabilizer, friction particles and solvent; The bacterial species of the microbial fermentation liquid is one or more of Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus, and the fermentation medium of the microbial fermentation liquid contains one or more of inulin, olive oil or glucose; the biological fermentation liquid is fermented to obtain a biological surfactant; The composite enzyme preparation is a mixture of protease, lipase and beta-(1,3-1,4)-glucanase.

2. The enzyme composition for oil pollution treatment according to claim 1, characterized in that The fermentation medium of the microbial fermentation liquid comprises the following raw materials in concentrations: 0.02-0.08 parts of zinc chloride, 0.01-0.03 parts of calcium chloride, 0.5-1.5 parts of potassium hydrogen phosphate, 0.2-0.7 parts of potassium dihydrogen phosphate, 0.5-1.5 parts of sodium chloride, 0.1-0.5 parts of calcium hydrogen carbonate and 5-15 parts of inulin.

3. The enzyme composition for oil pollution treatment according to claim 2, characterized in that: The bacterial species of the microbial fermentation liquid are a mixture of Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus, wherein the inoculation amount of Bacillus subtilis is 2-4%, the inoculation amount of Pseudomonas aeruginosa is 0.5-1% and the inoculation amount of Lactobacillus acidophilus is 1-3%.

4. The enzyme composition for oil pollution treatment according to claim 3, characterized in that: The method for preparing the microbial fermentation liquid comprises the following steps: S1. First, inoculate Bacillus subtilis, Pseudomonas aeruginosa and Lactobacillus acidophilus into Gao's medium respectively, at 30-35℃, 150-180r / min, activate and culture for 18-24h to obtain bacterial seed solution with a bacterial content of 10 7 -10 9 cfu / ml; S2. First, inoculate the bacterial seed liquid into the fermentation medium, culture at 25-35°C, 150-180r / min, and culture for 36-48h; then add 5% glucose by total weight, 25-35°C, 150-180r / min, and further culture for 18-24h, and concentrate to obtain the microbial fermentation liquid.

5. The enzyme composition for oil pollution treatment according to claim 1, characterized in that: The mass ratio of the protease, lipase and β-(1,3-1,4)-glucanase is (3-5):(3-5):(2-4).

6. The enzyme composition for oil pollution treatment according to claim 1, characterized in that: The preparation method of the anti-oil film-forming agent comprises the following steps: Step 1: First, place 10-30 ml of ethanol in a three-necked flask and heat it to 60-80° C. under a nitrogen atmosphere. After the temperature is constant, first add 0.5-1.5 ml of azobisisobutyronitrile-tetrahydrofuran solution, then add 1.0-1.4 g of NPCEMA, 3-5 g of MPC and 7-9 ml of azobisisobutyronitrile-tetrahydrofuran solution to 120-140 ml of anhydrous ethanol, and slowly dropwise add to the three-necked flask. After the dropwise addition is complete, add 0.5-1.5 ml of azobisisobutyronitrile-tetrahydrofuran solution again, stir for 5-7 hours, stop passing nitrogen, stir again for 15-20 hours, and finally rotary evaporate and dialyze to obtain a phosphorylcholine-containing polymer; Wherein, the concentration of azobisisobutyronitrile-tetrahydrofuran solution is 10.4 g / L; Step 2: Add 1-3g of phosphorylcholine-containing polymer to a three-necked round-bottom flask, add 40-60ml of ethanol, introduce nitrogen, heat to 50-70°C, add 10-30ml of dopamine hydrochloride-ethanol solution, the concentration of dopamine hydrochloride-ethanol solution is 25g / L, add triethylamine under nitrogen protection until the solution is neutral, react for 10-20h, adjust the pH value to 4 with hydrochloric acid, dialyze for 60-80h, and obtain an anti-oil film-forming agent.

7. The enzyme composition for oil pollution treatment according to claim 1, characterized in that: The stabilizer is sucrose ester, the solvent is N,N-dimethylformamide, and the friction particles are colored soft beads.

8. The enzyme composition for treating oil pollution according to claim 1, characterized in that: The enzyme composition for treating oil pollution comprises the following raw materials in parts by weight: 10-20 parts of microbial fermentation liquid, 5-7 parts of composite enzyme preparation, 3-6 parts of anti-oil pollution film-forming agent, 1-3 parts of stabilizer, 6-8 parts of friction particles and 10-12 parts of solvent.

9. A method for preparing the enzyme composition for oil pollution treatment according to any one of claims 1 to 8, characterized in that: The steps are: First, 10-20 parts of microbial fermentation liquid and 5-7 parts of composite enzyme preparation are dissolved in 100 parts of deionized water; 3-6 parts are dissolved in 10-12 parts of solvent; then the two solutions are mixed and stabilizer and friction particles are added in sequence, and finally 0.5-1.5 parts of emulsifier are added, emulsified and mixed evenly to obtain an enzyme composition for oil pollution treatment.

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