Portable cell culture equipment
By designing a portable cell culture device and using a multifunctional main box and related components, the problem of difficulty in culturing aerobic and anaerobic cells at the same time in the prior art is solved, and efficient cell culture and convenient equipment carrying are achieved.
Patent Information
- Application Number
- CN202411746311.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-02
- Publication Date
- 2025-02-25
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing cell culture technology is difficult to cultivate aerobic and anaerobic cells at the same time, resulting in poor culture effect and large equipment and inconvenient portability.
A portable cell culture device is designed, adopting a multi-functional main box, with a temperature regulating chamber, a pumping chamber and a storage chamber. Through components such as oxygen production machine, rotating fan blades and air pressure telescopic rods, the simultaneous culture of different types of cells is achieved, and the fixation of test tubes of different sizes is achieved through threaded screws and clamping frames.
Simultaneous culture of aerobic and anaerobic cells is achieved, which improves the culture effect, and reduces the volume and weight of the equipment through a portable design, enhancing the convenience of carrying and moving.
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Figure CN119506087A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of cell culture, in particular to a portable cell culture device. Background Art
[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions.
[0003] Among the existing cell culture technologies, most cell culture cannot achieve the simultaneous culture of aerobic cells and anaerobic cells, and thus different equipment is required for culture, which wastes a lot of culture costs and results in poor culture effects. In addition, the existing cell culture incubators are relatively large in size, making them inconvenient to carry and also have poor preservation effects. Summary of the invention
[0004] The object of the present invention is to provide a portable cell culture device to overcome the above-mentioned defects in the prior art.
[0005] According to the present invention, a portable cell culture device comprises a main box body, a chassis is fixedly provided at the lower end surface of the main box body, three groups of air extraction cavities are evenly distributed in the chassis and penetrate the chassis, a power frame is fixed on the inner wall of the air extraction cavity, a rotating shaft is rotatably provided at the lower end surface of the power frame, a rotating fan blade is fixedly provided on the outer circumference of the rotating shaft, so as to realize the function of extracting oxygen, a temperature regulating cavity is provided in the main box body, a temperature regulating tube is fixedly provided between the front and rear walls of the temperature regulating cavity, so as to achieve the function of regulating temperature, a load-bearing plate is fixedly provided on the inner circumference of the temperature regulating cavity, and the load-bearing plate is fixedly provided on the inner circumference of the temperature regulating cavity. Four groups of guide cavities are evenly distributed in the weight plate and penetrate the load-bearing plate, a blocking plate is fixedly provided on the upper side of the load-bearing plate, and four groups of storage cavities are evenly distributed on the upper end surface of the load-bearing plate. A threaded cavity opening outward is provided on the inner wall of the storage cavity away from the center and is interconnected with the storage cavity, a threaded screw is rotatably provided between the front and rear walls of the threaded cavity, and a threaded sleeve with opposite threads that can move towards each other is provided on the outer circular surface of the threaded screw, and a clamping frame is fixedly provided on the end surface of the threaded sleeve close to the center, so that the clamping frame serves to fix test tubes of different sizes.
[0006] As a further technical solution of the present invention, an oxygen production machine is fixedly provided on the inner wall of the air extraction chamber, and the oxygen production machine plays a role in producing oxygen. A rotating motor is fixedly provided on the upper wall of the air extraction chamber, and the upper end surface of the rotating shaft is dynamically connected to the rotating motor. When the rotating motor is started, the rotating shaft is driven to start rotating, and the rotating fan blades are driven to start rotating, thereby achieving the function of extracting oxygen.
[0007] As a further technical solution of the present invention, a pneumatic telescopic rod capable of telescopic movement is fixedly provided on the upper end surface of the load-bearing plate, and a control frame is fixedly provided on the other end of the pneumatic telescopic rod.
[0008] As a further technical solution of the present invention, a threaded motor is fixedly provided in the rear wall of the threaded cavity, and the rear end surface of the threaded cavity is dynamically connected to the threaded motor. When the threaded motor is started, the threaded screw starts to rotate, and the threaded sleeve and the clamping frame move toward each other, thereby achieving fixation of test tubes of different sizes.
[0009] As a further technical solution of the present invention, a load-bearing belt is fixedly provided on the end face of one side away from the center of the main box, and the load-bearing belt is easy to carry. A control panel is fixedly provided on the front end face of the main box, and the control panel plays a role in regulating the motor.
[0010] As a further technical solution of the present invention, a limit rack is fixedly provided on the inner wall of the storage cavity, the limit rack plays a limiting role, a sponge rack is fixedly provided inside the limit rack and penetrates the limit rack, and a replacement test tube is provided on the inner circular surface of the sponge rack.
[0011] As a further technical solution of the present invention, four groups of evenly distributed support columns are fixedly provided on the lower end surface of the chassis.
[0012] As a further technical solution of the present invention, four groups of electric doors that can be flipped and evenly distributed are provided on the upper side of the main box body. A glass frame is provided inside the electric door and passes through the electric door. The glass frame serves as an observation device. A rubber plate is provided inside the glass frame and passes through the glass frame.
[0013] As a further technical solution of the present invention, an electromagnet is fixedly provided on the lower end surface of the chassis, and an oxygen generator is provided on the outer cylindrical surface of the electromagnet. The oxygen generator cooperates with the electromagnet magnetically, thereby facilitating the replacement of the oxygen generator.
[0014] The beneficial effects of the present invention are:
[0015] 1. The present invention classifies the cultured cells according to whether they are anaerobic or aerobic. When the cells are aerobic, a temperature regulating tube is provided inside to start adjusting to the optimal temperature. At this time, the oxygen generated by the oxygen production machine is transported to the exhaust chamber and the temperature regulating chamber through the rotating fan blades, and then is absorbed by the cells. When anaerobic cells are present, the air pressure telescopic rod starts to stop moving, thereby ensuring that the control rack can block the mutual penetration of the guide chamber and the storage chamber, thereby ensuring that oxygen will not enter the storage chamber, thereby ensuring that the cell culture system can culture different types of cells at the same time.
[0016] 2. The present invention is provided with a threaded screw which starts to rotate, thereby driving the threaded screw to rotate, thereby pushing the threaded sleeve and the clamping frame to start moving toward each other. At this time, the inner side of the clamping frame contacts the test tube, thereby achieving precise fixation of test tubes of different sizes and shapes. A load-bearing belt is provided inside, and at this time the user only needs to pick up the load-bearing belt, thereby ensuring that the cell culture system can be quickly moved and carried. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 It is a left side view of the present invention;
[0018] Figure 2 is a top view of the present invention;
[0019] Figure 3 is a front view of the present invention;
[0020] Figure 4 The present invention Figure 3 Schematic diagram of AA in the middle;
[0021] Figure 5 The present invention Figure 3 Schematic diagram of the middle BB;
[0022] Figure 6 The present invention Figure 3 Schematic diagram of CC;
[0023] Figure 7 The present invention Figure 5 Schematic diagram of the appearance of the central control rack components.
[0024] In the figure:
[0025] 11. Main box; 12. Rotating fan blades; 13. Rotating shaft; 14. Rotating motor; 15. Oxygen generator; 16. Electromagnet; 17. Temperature control tube; 18. Temperature control chamber; 19. Blocking plate; 20. Chassis; 21. Guide chamber; 22. Limiting rack; 23. Sponge rack; 24. Replacement test tube; 25. Quantity control rack; 26. Pneumatic telescopic rod; 27. Load-bearing plate; 28. Storage chamber; 29. Threaded motor; 30. Threaded screw; 31. Threaded chamber; 32. Clamping rack; 33. Electric door; 34. Glass rack; 35. Load-bearing belt; 36. Threaded sleeve; 37. Power rack; 38. Vacuum chamber; 39. Support column; 40. Control panel; 41. Rubber sheet. DETAILED DESCRIPTION
[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention:
[0027] See attached Figure 1-Figure 7 According to an embodiment of the present invention, a portable cell culture device includes a main box 11, a chassis 20 is fixedly provided at the lower end surface of the main box 11, three groups of evenly distributed air extraction chambers 38 are provided in the chassis 20 and penetrate the chassis 20, a power frame 37 is fixed on the inner wall of the air extraction chamber 38, a rotating shaft 13 is rotatably provided on the lower end surface of the power frame 37, and a rotating fan blade 12 is fixedly provided on the outer circumference of the rotating shaft 13, so as to realize the function of extracting oxygen, a temperature regulating chamber 18 is provided in the main box 11, a temperature regulating tube 17 is fixedly provided between the front and rear walls of the temperature regulating chamber 18, so that the temperature regulating tube 17 plays a role in regulating temperature, and a load-bearing plate 27 is fixedly provided on the inner circumference of the temperature regulating chamber 18 The load-bearing plate 27 is provided with four groups of guide cavities 21 evenly distributed and passing through the load-bearing plate 27, a blocking plate 19 is fixedly provided on the upper side of the load-bearing plate 27, and four groups of storage cavities 28 evenly distributed are fixedly provided on the upper end surface of the load-bearing plate 27, and the inner wall of the storage cavity 28 away from the center is provided with a threaded cavity 31 opening outward and interpenetrating with the storage cavity 28, and a threaded screw 30 is rotatably provided between the front and rear walls of the threaded cavity 31, and a threaded sleeve 36 with opposite threads that can move toward each other is provided on the outer circumferential surface of the threaded screw 30, and a clamping frame 32 is fixedly provided on the end surface of the threaded sleeve 36 close to the center, so that the clamping frame 32 serves to fix test tubes of different sizes.
[0028] Among them, an oxygen production machine 15 is fixedly provided on the inner wall of the air extraction chamber 38, and the oxygen production machine 15 is used to produce oxygen. A rotating motor 14 is fixedly provided on the upper wall of the air extraction chamber 38, and the upper end surface of the rotating shaft 13 is dynamically connected to the rotating motor 14. When the rotating motor 14 is started, the rotating shaft 13 is driven to start rotating, and the rotating fan blades 12 are driven to start rotating, thereby realizing the function of extracting oxygen.
[0029] A pneumatic telescopic rod 26 capable of telescopic movement is fixedly provided on the upper end surface of the load-bearing plate 27 , and a control frame 25 is fixedly provided on the other end of the pneumatic telescopic rod 26 .
[0030] Among them, a threaded motor 29 is fixedly provided in the rear wall of the threaded cavity 31, and the rear end surface of the threaded cavity 31 is dynamically connected to the threaded motor 29. When the threaded motor 29 is started, the threaded screw 30 is driven to start rotating, and the threaded sleeve 36 and the clamping frame 32 are driven to move toward each other, thereby achieving the fixation of test tubes of different sizes.
[0031] A load-bearing belt 35 is fixedly provided on the end face of the main box body 11 away from the center, and the load-bearing belt 35 is easy to carry. A control panel 40 is fixedly provided on the front end face of the main box body 11, and the control panel 40 plays a role in regulating the motor.
[0032] A limit rack 22 is fixedly provided on the inner wall of the storage cavity 28 , and the limit rack 22 plays a limiting role. A sponge rack 23 is fixedly provided inside the limit rack 22 and penetrates the limit rack 22 , and a replacement test tube 24 is provided on the inner circular surface of the sponge rack 23 .
[0033] Among them, four groups of support columns 39 evenly distributed are fixedly provided on the lower end surface of the chassis 20 .
[0034] Among them, four groups of electric doors 33 that can be flipped and evenly distributed are provided on the upper side of the main box body 11. A glass frame 34 is provided inside the electric door 33 and passes through the electric door 33. The glass frame 34 plays an observation role. A rubber plate 41 is provided inside the glass frame 34 and passes through the glass frame 34.
[0035] The lower end surface of the chassis 20 is fixedly provided with an electromagnet 16, and the outer circumferential surface of the electromagnet 16 is provided with an oxygen generator 15. The oxygen generator 15 cooperates with the electromagnet 16 magnetically, thereby facilitating the replacement of the oxygen generator 15.
[0036] The portable cell culture device of the present invention has the following working process:
[0037] During operation, the user places the cell test tube that needs to be cultured on the inner surface of the sponge rack 23. At this time, the threaded motor 29 starts to start, thereby driving the threaded screw 30 to start rotating, thereby driving the threaded sleeve 36 and the clamping rack 32 to start moving toward each other. At this time, the inner side of the clamping rack 32 contacts the test tube, thereby achieving precise fixation of test tubes of different sizes and shapes.
[0038] When the cell tube storage is completed, the glass rack 34 is closed, and the storage chamber 28 forms a closed space. At this time, the user only needs to pick up the load-bearing belt 35, thereby ensuring that the cell culture system can be quickly moved and carried.
[0039] At the same time, the cultured cells are classified according to whether they are anaerobic or aerobic. When they are aerobic cells, the pneumatic telescopic rod 26 starts to start, thereby driving the quantity control frame 25 to start moving upward. At this time, the guide cavity 21 is interconnected with the storage cavity 28 and the temperature adjustment cavity 18. At this time, the rotating motor 14 starts to start, thereby driving the rotating shaft 13 to start rotating, thereby driving the rotating fan blades 12 to start rotating. At the same time, the temperature adjustment tube 17 starts to adjust to the optimal temperature. At this time, the oxygen generated by the oxygen production machine 15 is transported to the storage cavity 28 through the suction cavity 38 and the temperature adjustment cavity 18, and then absorbed by the cells. When anaerobic cells are present, the pneumatic telescopic rod 26 starts to stop, thereby ensuring that the quantity control frame 25 can block the mutual penetration of the guide cavity 21 and the storage cavity 28, thereby ensuring that oxygen will not enter the storage cavity 28, thereby ensuring that the cell culture system can culture different types of cells at the same time.
[0040] When the nutrient solution needs to be adjusted, the user directly penetrates the position of the test tube according to the glass frame 34, and the syringe penetrates the rubber plate 41 to inject the nutrient solution into the test tube.
[0041] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.
Claims
1. A portable cell culture device, comprising a main housing (11), characterized in that: A chassis (20) is fixedly provided on the lower end surface of the main housing (11), three groups of air extraction chambers (38) are evenly distributed and penetrate the chassis (20), a power frame (37) is fixed to the inner wall of the air extraction chamber (38), a rotating shaft (13) is rotatably provided on the lower end surface of the power frame (37), and a rotating fan blade (12) is fixedly provided on the outer circumferential surface of the rotating shaft (13), and a temperature adjustment chamber (18) is provided in the main housing (11); A temperature regulating tube (17) is fixedly provided between the front and rear walls of the temperature regulating chamber (18); a bearing plate (27) is fixedly provided on the inner circular surface of the temperature regulating chamber (18); four groups of guide cavities (21) are evenly distributed in the bearing plate (27) and penetrate the bearing plate (27); a blocking plate (19) is fixedly provided on the upper side of the bearing plate (27); and four groups of storage cavities (28) are evenly distributed on the upper end surface of the bearing plate (27).
2. The portable cell culture device according to claim 1, characterized in that: A threaded cavity (31) opening outward is provided on the inner wall of the storage cavity (28) away from the center and is mutually connected with the storage cavity (28). A threaded screw (30) is rotatably provided between the front and rear walls of the threaded cavity (31). A threaded sleeve (36) with opposite threads and capable of moving toward each other is provided on the outer circumference of the threaded screw (30). A clamping frame (32) is fixedly provided on the end surface of the threaded sleeve (36) close to the center.
3. The portable cell culture device according to claim 1, characterized in that: An oxygen generator (15) is fixedly provided on the inner wall of the air extraction chamber (38), a rotating motor (14) is fixedly provided on the upper wall of the air extraction chamber (38), and the upper end surface of the rotating shaft (13) is dynamically connected to the rotating motor (14).
4. The portable cell culture device according to claim 1, characterized in that: A pneumatic telescopic rod (26) capable of telescopic movement is fixedly provided on the upper end surface of the load-bearing plate (27), and a control frame (25) is fixedly provided on the other end of the pneumatic telescopic rod (26).
5. The portable cell culture device according to claim 2, characterized in that: A threaded motor (29) is fixedly disposed in the rear wall of the threaded cavity (31), and the rear end surface of the threaded cavity (31) is dynamically connected to the threaded motor (29).
6. The portable cell culture device according to claim 1, characterized in that: A load-bearing belt (35) is fixedly provided on an end surface of the main box body (11) away from the center, and a control panel (40) is fixedly provided on the front end surface of the main box body (11).
7. The portable cell culture device according to claim 1, characterized in that: A limiting frame (22) is fixedly provided on the inner wall of the storage cavity (28), a sponge frame (23) is fixedly provided inside the limiting frame (22) and penetrates the limiting frame (22), and a replacement test tube (24) is provided on the inner circular surface of the sponge frame (23).
8. The portable cell culture device according to claim 1, characterized in that: Four groups of evenly distributed support columns (39) are fixedly disposed on the lower end surface of the chassis (20).
9. The portable cell culture device according to claim 1, characterized in that: The upper side of the main box (11) is provided with four groups of electric doors (33) which can be turned over and are evenly distributed. A glass frame (34) is provided inside the electric door (33) and passes through the electric door (33). A rubber plate (41) is provided inside the glass frame (34) and passes through the glass frame (34).
10. The portable cell culture device according to claim 1, characterized in that: An electromagnet (16) is fixedly provided on the lower end surface of the chassis (20), an oxygen generator (15) is provided on the outer circumferential surface of the electromagnet (16), and the oxygen generator (15) cooperates with the electromagnet (16) in magnetic force.