Low-temperature storage device for Chinese ilex pollen

Abstract

The invention discloses a holly pollen low-temperature preservation device which comprises a shell, a pollen storage assembly is arranged in the shell, a plurality of blocking assemblies and rotating assemblies are arranged at the top of the shell, a refrigeration assembly is arranged on the inner side, close to the lower portion of the pollen storage assembly, of the shell, the pollen storage assembly comprises a containing shell, and a baffle is arranged on one side of the top of the containing shell; two handles are arranged on the outer wall of one side of the containing shell, sealing assemblies are arranged on the outer walls of the two sides of the end, close to the baffle, of the containing shell, a long plate is arranged on the inner wall of the containing shell, a plurality of rotating holes are formed in the long plate, a plurality of clamping bolts are arranged on the outer walls of the two sides of the containing shell, and a plurality of side openings are formed in the outer walls of the two sides of the containing shell. The hand grabbing disc is rotated to drive the top disc, the middle disc, the bottom disc and the ilex pollen sample pipe to rotate, and after the ilex pollen sample pipe rotates to the position under a pipe opening, pollen in the sample pipe is extracted in cooperation with an existing pollen pollinator, and the portability of operators is improved.

Description

Technical Field

[0001] The invention relates to the technical field of pollen preservation, in particular to a low-temperature preservation device for holly pollen. Background Art

[0002] Most holly plants are evergreen or sparsely deciduous, with beautiful tree shapes, diverse leaf shapes, bright and colorful fruit colors, and they persist in autumn and winter. They are excellent garden ornamental and urban greening plants. Some varieties are also excellent cut branch ornamental fruit trees and potted New Year flowers. Therefore, the market prospects for cultivating new holly varieties through hybrid breeding are broad. Pollen, as an important basic material for hybrid breeding, has rich genetic variation and is the key to the creation of new germplasm. A significant feature of holly plants is that most varieties are dioecious. In addition, the flowering periods of different holly plants vary greatly, up to several months. In order to improve the hybridization technology of holly plants and smoothly carry out the breeding of new holly varieties, long-term storage of pollen to maintain high vitality can effectively solve this problem. It is well known that under low temperature and low humidity conditions, the pollen of most plants can be stored for a long time and maintain vitality. Therefore, it is of great significance to study and invent a low-temperature storage device for holly pollen.

[0003] After the pollen is put into the storage bottle, in order to ensure the quality of the pollen and extend the storage time of the pollen, a bagged desiccant needs to be placed in the bottle, and a sealing film needs to be wrapped around the bottle mouth. In addition, the bottle containing pollen is generally stored in a low-temperature refrigerator. When in use, after the bottle is taken out of the refrigerator, the temperature in the bottle rises quickly, and the vitality of the pollen in the bottle is greatly reduced; and the existing pollen storage device usually adopts a flip-top method to open the pollen storage device, but because the operator needs to take out the sample tube containing pollen from the pollen storage device for pollen hybridization many times when performing hybridization pollination, the pollen storage device needs to be closed again after taking it out, and it needs to be opened again when the pollen is taken out next time, so it is necessary to repeat the operation of opening and closing the flip-top of the pollen storage device many times, which causes the operator to take out pollen from the pollen storage device when operating the pollen hybridization work, which brings inconvenience. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a holly pollen low-temperature storage device to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a holly pollen low-temperature preservation device, comprising a shell, a pollen storage component, a sealing component, a rotating component and a refrigeration component, wherein a pollen storage component is arranged inside the shell, a plurality of sealing components and a rotating component are arranged on the top of the shell, and a refrigeration component is arranged on the inner side of the shell near the bottom of the pollen storage component.

[0006] Furthermore, the pollen storage component includes a placement shell, a side opening, a sealing component, a long plate and a bolt. A baffle is provided on one side of the top of the placement shell, two handles are provided on the outer wall of one side of the placement shell, sealing components are provided on the outer walls on both sides of the placement shell near one end of the baffle, a long plate is provided on the inner wall of the placement shell, a plurality of rotating holes are provided on the long plate, a plurality of bolts are provided on the outer walls on both sides of the placement shell, and a plurality of side openings are opened on the outer walls on both sides of the placement shell.

[0007] Furthermore, the placement shell also includes a top plate, a middle plate and a bottom plate. The top plate is provided at the upper inner side of the placement shell, the bottom plate is provided above the inner side of the placement shell near the long plate, and the middle plate is provided between the top plate and the bottom plate.

[0008] Furthermore, the top plate includes a top groove, a support column and a fixed plate. A plurality of top grooves are opened on the top of the top plate, a plurality of support columns are arranged on both sides of the top plate, a fixed plate is arranged at the bottom of the support column, the fixed plate is connected to the bottom plate, and a plurality of bottom grooves are opened on the bottom plate.

[0009] Furthermore, the middle plate includes a connecting rod, a slider, a first spring assembly, a pressing strip and a middle groove. Connecting rods are provided on both sides of the middle plate. Two sliders are provided on the connecting rods. The sliders are embedded in the cavity of the support column and slide. A socket is provided at one end of the slider, and one end of the bolt is used to be inserted into the socket. The first spring assembly is connected between the slider and the cavity of the support column. A pressing strip is provided between the two sliders on the connecting rod, and a plurality of middle grooves are provided on the middle plate.

[0010] Furthermore, a top ring is provided on the circumferential outer wall of the top plate, and the top ring of the top plate is used to be embedded in the top groove and rotate. A number of top openings are opened on the top plate, and a number of upper insertion openings are also opened on the outer side of the top plate near the top openings. A rotating block is provided in the middle part of the top of the top plate, and two protrusions are provided on the circumferential outer wall of the rotating block.

[0011] Furthermore, a middle ring is provided on the outer circumferential wall of the middle disk, and the middle ring of the middle disk is used to be embedded in the middle groove and rotate. The middle disk is provided with a plurality of middle openings, and a plurality of insertion rods are provided on the outer side of the middle disk near the middle openings.

[0012] Furthermore, a bottom ring is provided on the circumferential outer wall of the lower part of the chassis, and the bottom ring of the chassis is used to be embedded in the bottom groove and rotate. A plurality of bottom openings are opened on the chassis, and a plurality of lower plug openings are provided on the upper part of the circumferential outer wall of the chassis. A rotating head is provided in the middle part of the bottom of the chassis, and the rotating head is used to be embedded in the rotating hole of the long plate and rotate. The sample tube is used to be inserted into the top opening and the middle opening and placed in the top opening, the middle opening and the bottom opening.

[0013] Furthermore, the refrigeration component includes an operation panel, a temperature sensor, a main control board, a transmitter, a relay, a refrigerator and a control module. The temperature sensor and the refrigerator are arranged on the inner side of the shell near the bottom of the pollen storage component. The transmitter and the main control board are located in the control module. A circuit is provided on the back of the temperature sensor, which is connected to the transmitter inside the control module. The transmitter is connected to the main control board, the main control board is connected to the relay, the relay is connected to the refrigerator, and the operation panel is arranged at the other end of the shell.

[0014] Furthermore, the rotating assembly includes a hand-grabbing plate, a connecting plate, a second spring assembly, a clamping head and a clamping plate. A plurality of pressure chambers are provided on the top wall of the outer shell, a pipe opening is provided on the top of the outer shell, and clamping grooves are provided on the inner walls on both sides of the upper part of the outer shell. The sealing assembly is used to be inserted into the pipe opening. Handles are provided on both sides of the outer shell. One end of the shaft rod of the hand-grabbing plate is used to pass through the pressure chamber and the connecting plate and be inserted into the outer shell. A clamping head is provided on the end of the shaft rod inserted into the outer shell. A second spring assembly is provided between the connecting plate and the pressure chamber. The connecting plate and the clamping head are movably connected. A plurality of clamping plates are provided on the inner wall of the clamping head. The clamping head is used to be sleeved on the periphery of the rotating block and cooperate with each other.

[0015] Compared with the existing technology, the present invention has the following beneficial effects:

[0016] (1) After the temperature sensor of the refrigeration component detects that the temperature inside the shell reaches a constant temperature, the stored holly pollen is stored at a low temperature, which effectively prolongs the storage time of the holly pollen and solves the problem of the flowering period. (2) The present invention seals the pipe mouth of the shell through a sealing component to prevent the loss of cold air from the shell and improve the constant temperature effect of the shell. (3) The present invention drives the clamping head and the rotating block to rotate by rotating the hand-grabbing plate. The rotating block drives the top plate, the middle plate, the bottom plate and the sample tube containing holly pollen to rotate while rotating, so that the sample tube containing holly pollen rotates to the bottom of the pipe mouth, and then extracts pollen from the sample tube containing holly pollen through the pipe mouth. When extracting pollen, only one sealing component needs to be pulled out from the pipe mouth, and then cooperate with the existing pollen pollinator to extract the pollen in the sample tube, thereby improving the portability of the operator. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of the low-temperature storage device of the present invention.

[0018] Figure 2 This is a schematic diagram of the exploded three-dimensional structure of the pollen storage component.

[0019] Figure 3 It is a schematic diagram of the three-dimensional structure of the top plate, the middle plate and the bottom plate connected by rods according to the present invention.

[0020] Figure 4 It is a schematic diagram of the exploded three-dimensional structure of the top plate, the middle plate and the bottom plate of the present invention.

[0021] Figure 5 It is a schematic diagram of the three-dimensional structure in which the rods between the top plate, the middle plate and the bottom plate are not connected.

[0022] Figure 6 It is a schematic diagram of the rotating three-dimensional structure of the rotating component and the pollen storage component of the present invention.

[0023] Figure 7 It is a schematic diagram of the three-dimensional structure of the rotating assembly and the pollen storage assembly of the present invention when rotating from another side.

[0024] Figure 8 It is a schematic diagram of the enlarged plan structure of A of the present invention.

[0025] Fig. 9 It is a schematic diagram of the planar structure of the refrigeration component of the present invention.

[0026] Fig.10 It is a schematic diagram of the planar structure of the operating panel on the other side of the housing of the present invention.

[0027] In the figure: 1, housing; 101, slot; 2, pollen storage component; 21, placement shell; 201, side opening; 202, sealing component; 203, baffle; 204, long board; 22, latch; 23, top plate; 2301, top slot; 2302, support column; 2303, fixing plate; 24, middle plate; 2401, connecting rod; 2402, slider; 2403, first spring component; 2404, pressing strip; 2405, middle slot; 25, bottom plate; 2501, bottom slot; 26, top plate; 2601, top opening; 2602, upper socket; 2603, top ring; 2604, rotating block; 27, middle plate; 2701, plug rod; 2702, middle ring; 2703, middle opening; 28, bottom plate; 2801, bottom opening; 2802, lower socket; 2803, bottom ring; 2804, rotating head; 29, sample tube; 3, plugging assembly; 31, tube opening; 4, rotating assembly; 41, hand-gripping plate; 42, connecting plate; 43, second spring assembly; 44, clamp; 4401, clamping plate; 5, refrigeration assembly. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below with reference to implementation cases and accompanying drawings.

[0029] like Figure 1 As shown, a holly pollen low-temperature preservation device includes a shell 1, a pollen storage component 2, a sealing component 3, a rotating component 4 and a refrigeration component 5. The pollen storage component 2 is arranged in the shell 1, a plurality of sealing components 3 and a rotating component 4 are arranged on the top of the shell 1, and a refrigeration component 5 is arranged on the inner side of the shell 1 near the bottom of the pollen storage component 2.

[0030] like Figure 2As shown, the pollen storage component 2 includes a placement shell 21, a side opening 201, a sealing component 202, a long plate 204 and a bolt 22. A baffle 203 is provided on one side of the top of the placement shell 21. Two handles are provided on one side of the outer wall of the placement shell 21. Sealing components 202 are provided on both sides of the outer wall of the placement shell 21 near one end of the baffle 203. A long plate 204 is provided on the inner wall of the placement shell 21. A plurality of rotating holes are provided on the long plate 204. A plurality of bolts 22 are provided on both sides of the outer wall of the placement shell 21. Several side openings 201; if holly pollen needs to be stored, first grasp the two handles and pull them outward, and use the handles to pull the placement shell 21 out from the inside of the outer shell 1, so that the placement shell 21 drives the top plate 23, the middle plate 24 and the bottom plate 25 to move out from the inside of the outer shell 1. At this time, the lower end of the sample tube 29 containing holly pollen can be inserted from the top opening 2601, so that the lower end of the sample tube 29 passes through the top opening 2601 and the middle opening 2703 and is placed inside the top opening 2601, the middle opening 2703 and the bottom opening 2801, and so on, until the sample tube 29 is filled.

[0031] The placement shell 21 further includes a top plate 23, a middle plate 24 and a bottom plate 25. The top plate 23 is provided on the upper inner side of the placement shell 21, the bottom plate 25 is provided on the inner side of the placement shell 21 near the upper part of the long plate 204, and the middle plate 24 is provided between the top plate 23 and the bottom plate 25.

[0032] like Figure 3 As shown, the top plate 23 includes a top groove 2301, a support column 2302 and a fixed plate 2303. A plurality of top grooves 2301 are opened on the top of the top plate 23, a plurality of support columns 2302 are arranged on both sides of the top plate 23, a fixed plate 2303 is arranged at the bottom of the support column 2302, the fixed plate 2303 is connected to the bottom plate 25, and a plurality of bottom grooves 2501 are opened on the bottom plate 25.

[0033] Among them, the middle plate 24 includes a connecting rod 2401, a slider 2402, a first spring assembly 2403, a pressing strip 2404 and a middle groove 2405. Connecting rods 2401 are provided on both sides of the middle plate 24. Two sliders 2402 are provided on the connecting rod 2401. The slider 2402 is embedded in the cavity of the support column 2302 and slides. A socket is opened at one end of the slider 2402, and one end of the bolt 22 is used to be inserted into the socket. The first spring assembly 2403 is connected between the slider 2402 and the cavity of the support column 2302. A pressing strip 2404 is provided between the two sliders 2402 on the connecting rod 2401, and a plurality of middle grooves 2405 are opened on the middle plate 24.

[0034] like Figure 4-Figure 5As shown, a top ring 2603 is provided on the circumferential outer wall of the top plate 26, and the top ring 2603 of the top plate 26 is used to be embedded in the top groove 2301 and rotate. A plurality of top openings 2601 are opened on the top plate 26, and a plurality of upper sockets 2602 are also opened on the outer side of the top plate 26 near the top openings 2601. A rotating block 2604 is provided in the middle part of the top of the top plate 26, and two protrusions are provided on the circumferential outer wall of the rotating block 2604.

[0035] A middle ring 2702 is provided on the outer circumferential wall of the middle plate 27 . The middle ring 2702 of the middle plate 27 is used to be inserted in the middle groove 2405 for rotation. A plurality of middle openings 2703 are opened on the middle plate 27 . A plurality of insertion rods 2701 are provided on the outer side of the middle plate 27 near the middle openings 2703 .

[0036] Among them, a bottom ring 2803 is provided on the circumferential outer wall of the lower part of the chassis 28, and the bottom ring 2803 of the chassis 28 is used to be embedded in the bottom groove 2501 and rotated, a plurality of bottom openings 2801 are opened on the chassis 28, and a plurality of lower plug openings 2802 are provided on the upper part of the circumferential outer wall of the chassis 28. A rotating head 2804 is provided in the middle part of the bottom of the chassis 28, and the rotating head 2804 is used to be embedded in the rotating hole of the long plate 204 and rotated, and the sample tube 29 is used to be inserted into the top opening 2601 and the middle opening 2703 and placed in the top opening 2601, the middle opening 2703 and the bottom opening 2801.

[0037] Among them, the upper and lower ends of the insertion rod 2701 are used to be inserted into the upper socket 2602 and the lower socket 2802 respectively; after the sample tube 29 is fully inserted, the pressing strip 2404 is pressed downward, so that the pressing strip 2404 drives the two connecting rods 2401 to move downward, and the connecting rod 2401 moves downward while also driving the two sliders 2402 to slide downward along the cavity of the support column 2302, and the socket at one end of the slider 2402 will move downward along the side opening 201. At the same time, when the connecting rod 2401 moves downward, it also drives the middle plate 24 to move downward, so that the middle plate 24 drives the middle plate 27 and the insertion rod 2701 to move downward, and one end of the insertion rod 2701 that moves downward will be inserted into the lower socket 2802 of the bottom plate 28, so that the top plate 26, the middle plate 27 and the bottom plate 28 are connected together, which is convenient for the subsequent rotation of the top plate 26, the middle plate 27 and the bottom plate 28;

[0038] After the top plate 26, the middle plate 27 and the bottom plate 28 are connected, one end of the clamping bolt 22 can be moved toward the socket of the slider 2402, and one end of the clamping bolt 22 can be inserted into the socket of the slider 2402. Similarly, all four clamping bolts 22 of the placement shell 21 are inserted into the sockets of the slider 2402 to fix the middle plate 24 that moves downward;

[0039] After the middle plate 24 is fixed, the handle can be grasped again and pushed inward by the handle to push the placement shell 21 back into the outer shell 1 so that the rotating block 2604 of the top plate 26 stays just below the clamping head 44.

[0040] like Figure 6-Figure 8As shown, the rotating assembly 4 includes a hand-gripping plate 41, a connecting plate 42, a second spring assembly 43, a clamping head 44 and a clamping plate 4401. A plurality of pressure chambers are provided on the top wall of the shell 1. A pipe opening 31 is provided on the top of the shell 1. Clamping grooves 101 are provided on the inner walls on both sides of the upper part of the shell 1. The blocking assembly 3 is used to be inserted into the pipe opening 31. Handles are provided on both sides of the shell 1. One end of the shaft rod of the hand-gripping plate 41 is used to pass through the pressure chamber and the connecting plate 42 and be inserted into the shell 1. A clamping head 44 is provided on the end of the shaft rod inserted into the shell 1. A second spring assembly 43 is provided between the connecting plate 42 and the pressure chamber. The connecting plate 42 and the clamping head 44 are movably connected. A plurality of clamping plates 4401 are provided on the inner wall of the clamping head 44. The clamping head 44 is used to be sleeved on the rotating block 2 604 cooperate with each other; if it is necessary to take out the pollen for use, since the top plate 26, the middle plate 27 and the bottom plate 28 are previously connected together by the insertion rod 2701, when it is necessary to take out the pollen from the sample tube 29, the first blocking component 3 can be pulled out from the first top pipe opening 31 of the shell 1, and the existing pollen pollinator can be used to extend from the pipe opening 31 into the sample tube 29 to extract the holly pollen in the sample tube 29. After the holly pollen in the first sample tube 29 is extracted, at this time, the hand grasping plate 41 can be grasped and pressed downward, so that the shaft rod of the hand grasping plate 41 drives the connecting plate 42 and the clamping head 44 to move downward, and the clamping head 44 will be sleeved on the periphery of the rotating block 2604. At the same time, the connecting plate 42 will move downward while the first The second spring assembly 43 is stretched downward, and after the clamping head 44 is sleeved on the periphery of the rotating block 2604, the hand gripping plate 41 can be rotated to make the hand gripping plate 41 rotate to drive the shaft rod and the clamping head 44 to rotate. While the clamping head 44 rotates, it also drives the clamping plate 4401 to rotate. After the clamping plate 4401 rotates a certain distance, the clamping plate 4401 will contact the protrusion of the clamping head 44. As the hand gripping plate 41 is continuously rotated, the clamping plate 4401 will push the protrusion of the clamping head 44 to continue to rotate. At this time, the rotating head 2804 of the chassis 28 will rotate in the rotating hole of the long plate 204, turning the first sample tube 29 from the bottom of the first pipe mouth 31, and rotating the second sample tube 29 to the bottom of the first pipe mouth 31. At this time, The existing pollen pollinator is used to extend from the first pipe opening 31 into the second sample tube 29 to extract the holly pollen in the second sample tube 29, and so on. Each top plate 26 is provided with five top openings 2601, and the five top openings 2601 can store five sample tubes 29 filled with holly pollen. After the five sample tubes 29 filled with holly pollen are extracted, the previous first plugging component 3 can be reinserted into the first pipe opening 31. At this time, the second plugging component 3 can be pulled out from the second pipe opening 31 to continue to extract holly pollen. When extracting pollen, only one plugging component 3 needs to be pulled out from the pipe opening 31, and the pollen in the sample tube 29 can be extracted in cooperation with the existing pollen pollinator, thereby improving the portability of the operator;If the pollen storage device is kept open until the operator takes out all the pollen from the pollen storage device and completes pollen hybridization, and then closes the pollen storage device, it will affect the cooling effect inside the pollen storage device and cause the internal temperature of the pollen storage device to rise. Therefore, more energy is needed to maintain the temperature, increasing energy consumption. In severe cases, the internal cooling function of the pollen storage device may be damaged. ;

[0041] like Figure 9-10 As shown, the refrigeration component 5 includes an operation panel, a temperature sensor, a main control board, a transmitter, a relay, a refrigerator and a control module. The temperature sensor and the refrigerator are arranged on the inner side of the shell 1 near the bottom of the pollen storage component 2. The transmitter and the main control board are located in the control module. A circuit is provided on the back of the temperature sensor, which is connected to the transmitter inside the control module. The transmitter is connected to the main control board, the main control board is connected to the relay, the relay is connected to the refrigerator, and the operation panel is arranged at the other end of the shell 1; after the placement shell 21 is pushed back into the shell 1, if the stored holly pollen needs to be stored at a low temperature, the PT100 temperature sensor detects the temperature inside the shell 1, and transmits the temperature data signal to the transmitter inside the control module through the back circuit, the transmitter performs ADC adoption work, and then The sampled data signal is input to the single-chip microcomputer inside the control module, and the single-chip microcomputer displays the real-time temperature value on the LED screen on the operation panel. When the temperature inside the shell 1 is high, the main control board inside the control module will give an electrical signal to the connected relay, which will cause the relay to start working and connect the circuit where the PTC refrigerator is located. At this time, the PTC refrigerator embedded in the shell 1 starts working. When the PT100 temperature sensor detects that the temperature inside the shell 1 reaches a constant temperature, the stored holly pollen is stored at a low temperature, and the constant temperature is 4°C and -20°C. The PT100 temperature sensor transmits the signal to the main control board inside the control module, and the main control board will give a signal to the relay to stop the relay from working, disconnect the circuit where the PTC refrigerator is located, and stop cooling in the shell 1.

[0042] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A holly pollen cryopreservation device, comprising a housing (1), a pollen storage component (2), a blocking component (3), a rotating component (4) and a refrigeration component (5), characterized in that: A pollen storage component (2) is arranged inside the shell (1), a plurality of blocking components (3) and a rotating component (4) are arranged on the top of the shell (1), and a refrigeration component (5) is arranged on the inner side of the shell (1) near the bottom of the pollen storage component (2).

2. The holly pollen cryopreservation device according to claim 1, characterized in that: The pollen storage component (2) comprises a placement shell (21), a side opening (201), a sealing component (202), a long plate (204) and a latch (22); a baffle (203) is provided on one side of the top of the placement shell (21); two handles are provided on one side of the outer wall of the placement shell (21); sealing components (202) are provided on both sides of the outer walls of the placement shell (21) close to one end of the baffle (203); a long plate (204) is provided on the inner wall of the placement shell (21); a plurality of rotating holes are provided on the long plate (204); a plurality of latches (22) are provided on both sides of the outer walls of the placement shell (21); and a plurality of side openings (201) are provided on both sides of the outer walls of the placement shell (21).

3. A holly pollen cryopreservation device according to claim 2, characterized in that: The placement shell (21) further comprises a top plate (23), a middle plate (24) and a bottom plate (25); the top plate (23) is provided at the upper inner portion of the placement shell (21); the bottom plate (25) is provided at the upper inner portion of the placement shell (21) near the long plate (204); and the middle plate (24) is provided between the top plate (23) and the bottom plate (25).

4. A holly pollen cryopreservation device according to claim 3, characterized in that: The top plate (23) comprises a top groove (2301), a support column (2302) and a fixed plate (2303); a plurality of top grooves (2301) are provided on the top of the top plate (23); a plurality of support columns (2302) are provided on both sides of the top plate (23); a fixed plate (2303) is provided at the bottom of the support column (2302); the fixed plate (2303) is connected to the bottom plate (25); and a plurality of bottom grooves (2501) are provided on the bottom plate (25).

5. The holly pollen cryopreservation device according to claim 4, characterized in that: The middle plate (24) comprises a connecting rod (2401), a slider (2402), a first spring assembly (2403), a pressing strip (2404) and a middle groove (2405). Connecting rods (2401) are provided on both sides of the middle plate (24). Two sliders (2402) are provided on the connecting rod (2401). The sliders (2402) are embedded in the cavity of the support column (2302) for sliding. A socket is provided at one end of the slider (2402). One end of the latch (22) is used to be inserted into the socket. The first spring assembly (2403) is connected between the slider (2402) and the cavity of the support column (2302). A pressing strip (2404) is provided between the two sliders (2402) on the connecting rod (2401). A plurality of middle grooves (2405) are provided on the middle plate (24).

6. The holly pollen cryopreservation device according to claim 5, characterized in that: A top ring (2603) is provided on the circumferential outer wall of the top plate (26), and the top ring (2603) of the top plate (26) is used to be embedded in the top groove (2301) and rotate. A plurality of top openings (2601) are provided on the top plate (26), and a plurality of upper insertion openings (2602) are also provided on the outer side of the top plate (26) near the top openings (2601). A rotating block (2604) is provided in the middle of the top of the top plate (26), and two protrusions are provided on the circumferential outer wall of the rotating block (2604).

7. The holly pollen cryopreservation device according to claim 6, characterized in that: A middle ring (2702) is provided on the circumferential outer wall of the middle plate (27), and the middle ring (2702) of the middle plate (27) is used to be embedded in the middle groove (2405) and rotate. The middle plate (27) is provided with a plurality of middle openings (2703), and a plurality of insertion rods (2701) are provided on the outer side of the middle plate (27) near the middle openings (2703).

8. The holly pollen cryopreservation device according to claim 7, characterized in that: A bottom ring (2803) is provided on the circumferential outer wall of the lower part of the chassis (28), and the bottom ring (2803) of the chassis (28) is used to be embedded in the bottom groove (2501) and rotated. A plurality of bottom openings (2801) are opened on the chassis (28), and a plurality of lower insertion openings (2802) are provided on the upper part of the circumferential outer wall of the chassis (28). A rotating head (2804) is provided in the middle part of the bottom of the chassis (28), and the rotating head (2804) is used to be embedded in the rotating hole of the long plate (204) and rotated. The sample tube (29) is used to be inserted into the top opening (2601) and the middle opening (2703) and placed in the top opening (2601), the middle opening (2703) and the bottom opening (2801).

9. The holly pollen cryopreservation device according to claim 8, characterized in that: The refrigeration component (5) comprises an operation panel, a temperature sensor, a main control board, a transmitter, a relay, a refrigerator and a control module. The temperature sensor and the refrigerator are arranged on the inner side of the housing (1) near the bottom of the pollen storage component (2). The transmitter and the main control board are located in the control module. A circuit is provided on the back of the temperature sensor and is connected to the transmitter inside the control module. The transmitter is connected to the main control board, the main control board is connected to the relay, and the relay is connected to the refrigerator. The operation panel is arranged at the other end of the housing (1).

10. The holly pollen cryopreservation device according to claim 9, characterized in that: The rotating assembly (4) comprises a hand-gripping plate (41), a connecting plate (42), a second spring assembly (43), a clamping head (44) and a clamping plate (4401). A plurality of pressure chambers are provided on the top wall of the shell (1). A pipe opening (31) is provided on the top of the shell (1). Clamping grooves (101) are provided on the inner walls on both sides of the upper part of the shell (1). The blocking assembly (3) is used to be inserted into the pipe opening (31). Handles are provided on both sides of the shell (1). One end of the shaft rod of the hand-gripping plate (41) is used to pass through the pressure chamber and the connecting plate (42) and be inserted into the shell (1). A clamping head (44) is provided on the end of the shaft rod inserted into the shell (1). A second spring assembly (43) is provided between the connecting plate (42) and the pressure chamber. The connecting plate (42) and the clamping head (44) are movably connected. A plurality of clamping plates (4401) are provided on the inner wall of the clamping head (44). The clamping head (44) is used to be sleeved on the outer periphery of the rotating block (2604) to cooperate with each other.

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