Composition containing probiotics as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of preparation of probiotics and medicinal and edible raw materials, and particularly relates to a composition containing probiotics as well as a preparation method and application of the composition. The composition containing the probiotics is prepared from the following raw materials: 3 to 20 parts of fructus crataegi, 0.1 to 2 parts of endothelium corneum gigeriae galli, 0.3 to 3 parts of poria cocos, 0.5 to 5 parts of malt, 0.2 to 2 parts of lactobacillus rhamnosus GG, 0.2 to 2 parts of bifidobacterium animalis subsp. Lactis HN019, 0.02 to 1 part of lactobacillus fermentum CECT5716 and 0.02 to 1 part of lactobacillus rhamnosus HN001. The solid composition based on probiotics provided by the invention has the effects of regulating gastrointestinal functions, relaxing bowel and improving and promoting immunity. And the suspension which is fine, free of granular sensation and relatively uniform in dispersion can be formed only by brewing with warm water. The composition is not easy to cake in the transportation and storage processes, can maintain a relatively good number of living bacteria, and has a good application value.

Description

Technical Field

[0001] The invention belongs to the technical field of preparation of probiotics and medicinal and edible raw materials, and particularly relates to a composition containing probiotics, a preparation method and application thereof. Background Art

[0002] Bad eating habits and lifestyles, such as excessive stress, abuse of antibiotics, frequent drinking, smoking, frequent nighttime eating, and a preference for unhealthy foods such as barbecue and hot pot, can disrupt the balance of intestinal flora, which in turn has a profound and serious negative impact on intestinal health. For example, long-term drinking not only directly damages the liver, but can also lead to alcoholic liver disease, hepatitis, and even cirrhosis. When the liver is damaged, the body's detoxification function and immunity will also be weakened, making people more susceptible to diseases such as inflammation.

[0003] Probiotics are active microorganisms that are beneficial to the host by changing the composition of the host's flora in a certain part of the human body. They promote nutrient absorption and maintain intestinal health by regulating the host's mucosal and systemic immune functions or by regulating the balance of intestinal flora, thereby producing single microorganisms or mixed microorganisms with clear composition that are beneficial to health. Probiotics change the interaction between intestinal microorganisms, improve digestion and absorption and intestinal barrier function, and provide protection for the health of the body through their adhesion to the intestine and competitive inhibition of harmful bacteria.

[0004] Currently, probiotic preparations are generally made into powders and tablets, and are often consumed with food when used. However, there are technical problems such as large loss of bacteria, inconvenient storage, and serious attenuation during transportation, which leads to reduced health-care activity. Summary of the invention

[0005] In view of the problems existing in the prior art, the object of the present invention is to provide a composition containing probiotics and its preparation method and application. Specifically, the solid composition based on probiotics provided by the present invention has the effects of regulating gastrointestinal function, moistening the intestines and promoting defecation, and improving immunity. At the same time, the composition containing probiotics does not need to add auxiliary ingredients such as foaming agents, and only warm water can be used to brew to form a delicate, non-granular, and evenly dispersed suspension. And the solid composition is not easy to stick and clump during transportation and storage, and can maintain a good bacterial viability. Based on the above research results, the present invention is completed.

[0006] Specifically, the technical solution of the present invention is as follows: The first aspect of the present invention provides a composition containing probiotics, wherein the raw materials include 3-20 parts of hawthorn, 0.1-2 parts of chicken gizzard lining, 0.3-3 parts of poria, 0.5-5 parts of malt, 0.2-2 parts of Lactobacillus rhamnosus GG, 0.2-2 parts of Bifidobacterium animalis lactis subsp. HN019, 0.02-1 parts of Lactobacillus fermentum CECT5716, and 0.02-1 parts of Lactobacillus rhamnosus HN001.

[0007] The present invention achieves a synergistic effect between the probiotic supplement and the Chinese medicinal materials through the compatibility of probiotics, hawthorn, chicken gizzard lining, Poria cocos and malt, which can not only exert the pharmacological activity of a single probiotic, Chinese medicinal material, etc., but also exert the efficacy between the probiotics and the Chinese medicinal materials.

[0008] According to the present invention, preferably, the probiotics are live bacteria, and the total live bacteria count is not less than 10 billion cfu / g.

[0009] The second aspect of the present invention provides a method for preparing the composition containing probiotics according to the first aspect, comprising: crushing and mixing hawthorn, chicken gizzard lining, poria cocos and malt respectively to obtain a mixed raw material; Add probiotics to the mixed raw materials, mix well and package.

[0010] Furthermore, the probiotic composition can be obtained by subpackaging. In a specific embodiment of the present invention, each bag of the probiotic composition contains 0.5-5 g, and further contains 1 g. The probiotic composition is a loose powder with a white main body and a red doped part.

[0011] The probiotics are in the form of solid powder.

[0012] The hawthorn and chicken gizzard lining are sieved with 80-100 meshes, the poria and malt are sieved with 120-150 meshes, and the solid probiotic powder is sieved with 50-70 meshes. The present invention has found through research that by controlling the dosage ratio and particle size of the above-mentioned medicinal and edible raw materials and probiotic powder, it is helpful to improve its storage stability, so that the solid composition is not easy to agglomerate, and it is also helpful to form a fine, non-granular, more evenly dispersed and better-tasting suspension when re-dissolving with water.

[0013] The third aspect of the present invention provides the use of the probiotic-containing composition described in the first aspect in the preparation of products for regulating gastrointestinal function, moistening the intestines and promoting bowel movements, and improving immunity.

[0014] Beneficial technical effects of one or more of the above technical solutions: 1. In the probiotic composition provided by the above technical solution, the probiotics and the medicinal and edible raw materials are effectively compounded, which has the effects of relieving intestinal inflammation and regulating gastrointestinal function, and has the effect of relieving constipation caused by gastrointestinal dysfunction. At the same time, it can also enhance the body's immune activity and has immune regulation ability.

[0015] 2. The probiotic composition provided by the above technical solution can regulate the stomach and intestines, moisturize the intestines and promote bowel movements through the combination of specific medicinal and edible raw materials and probiotics. The ratio of the number of live bacteria of Lactobacillus rhamnosus GG, Bifidobacterium animalis lactis subspecies HN019, Lactobacillus fermentum CECT5716, and Lactobacillus rhamnosus HN001 is 50:50:1:1, which is conducive to colonization in the intestine, inhibiting harmful bacteria, promoting the secretion of beneficial bacteria, and enhancing intestinal immunity; it can also effectively regulate the activity of macrophages in the immune system, thereby significantly enhancing human immunity.

[0016] 3. The probiotic-containing composition provided by the above technical solution has moderate types and contents of probiotics, can achieve good gastrointestinal function regulation and immune regulation effects, and can effectively moisturize the intestines and promote bowel movements.

[0017] 4. The probiotic-containing composition provided by the above technical solution can form a delicate, particle-free, evenly dispersed suspension with a good taste without the need to add a foaming aid during the preparation process.

[0018] 5. The probiotic-containing composition provided by the above technical solution is not easy to agglomerate during transportation and storage, can be stored for a long time, and has good market application value.

[0019] 6. The probiotic-containing composition provided by the above technical solution can regulate intestinal flora and can be widely used in functional health foods such as gastrointestinal function regulation, immune regulation, and bowel-moistening and laxative. DETAILED DESCRIPTION

[0020] It should be noted that the following detailed descriptions are all illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0021] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprising" and / or "including" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0022] A typical embodiment of the present invention provides a composition containing probiotics, comprising the following raw materials in parts by weight: 3-20 parts of hawthorn, 0.1-2 parts of chicken gizzard lining, 0.3-3 parts of poria, 0.5-5 parts of malt, 0.2-2 parts of Lactobacillus rhamnosus GG, 0.2-2 parts of Bifidobacterium animalis lactis subsp. HN019, 0.02-1 parts of Lactobacillus fermentum CECT5716, and 0.02-1 parts of Lactobacillus rhamnosus HN001.

[0023] The present invention achieves a synergistic effect between the probiotic supplement and the Chinese medicinal materials through the compatibility of probiotics, hawthorn, chicken gizzard lining, Poria cocos and malt, which can not only exert the pharmacological activity of a single probiotic, Chinese medicinal material, etc., but also exert the medicinal effects between the probiotics and the Chinese medicinal materials.

[0024] Hawthorn can strengthen the spleen and aid digestion, promote blood circulation and remove blood stasis, clear turbidity and reduce fat, and regulate intestinal flora; chicken's gizzard lining can strengthen the spleen and stomach, help digestion and reduce phlegm, can stimulate the gastric mucosa, promote gastric juice secretion, and aid digestion; Poria has the effects of promoting diuresis and dampness, tonifying the middle and strengthening the spleen, promoting gastrointestinal fluid secretion, anti-bacterial, anti-ulcer, and enhancing immunity; malt has the effects of promoting qi and aiding digestion, strengthening the spleen and stimulating appetite.

[0025] Lactobacillus rhamnosus GG can enhance immune function, regulate intestinal health, protect gastric mucosa, promote microecological balance, etc. It can resist the invasion of intestinal pathogens and has strong biosafety and probiotic properties.

[0026] Animal Bifidobacterium lactis subspecies HN019 can regulate the immune system, maintain mucosal homeostasis, has good impedance to viruses, fungi and bacteria, and significantly enhances human immunity.

[0027] Fermented Lactobacillus CECT5716 can prevent and treat diarrhea, restore the normal structure of the digestive tract, promote intestinal mucus secretion, improve the intestinal resistance to exogenous substances, prevent pathogens from invading damaged cells and mucosa, promote the proliferation of macrophages and lymphocytes, induce mucosal immunity, has excellent prebiotic properties, inhibits the formation of intestinal endotoxins, and has a significant effect on intestinal immunity regulation.

[0028] Lactobacillus rhamnosus HN001 can improve the body's immunity, reduce cholesterol and triglyceride levels, increase the ratio of bifidobacteria and lactobacilli in the intestines of patients with cholelithiasis, reduce pathogenic bacteria, and regulate the types and proportions of beneficial intestinal bacteria. It plays an important role in maintaining the balance of oxidation and anti-oxidation in the body and delaying the aging of the body. According to the present invention, preferably, the raw materials include 10 parts of hawthorn, 1 part of chicken gizzard lining, 2 parts of poria, 3 parts of malt, 0.5 parts of Lactobacillus rhamnosus GG, 0.5 parts of Bifidobacterium animalis lactis subsp. HN019, 0.05 parts of Lactobacillus fermentum CECT5716, and 0.05 parts of Lactobacillus rhamnosus HN001.

[0029] According to the present invention, preferably, all probiotics are live bacteria, wherein the ratio of the live bacteria count of Lactobacillus rhamnosus GG, Bifidobacterium animalis lactis subsp. HN019, Lactobacillus fermentum CECT5716 and Lactobacillus rhamnosus HN001 is 50:50:0.5-5:0.5-5 (preferably 50:50:1:1). The live bacteria count of the total probiotics is not less than 10 billion cfu / g, further 10-20 billion cfu / g.

[0030] In another specific embodiment of the present invention, a method for preparing the probiotic-containing composition is provided, comprising: crushing and mixing hawthorn, chicken gizzard lining, poria cocos and malt respectively to obtain a mixed raw material; Add probiotics to the mixed raw materials, mix well and package.

[0031] In another embodiment of the present invention, the probiotic composition can be obtained by subpackaging. In one embodiment of the present invention, each bag of the probiotic composition is 0.5-5g, further 1g. The probiotic composition is mainly white loose powder mixed with red powder particles.

[0032] The probiotics are a mixed bacterial agent of the above-mentioned Lactobacillus rhamnosus GG, Bifidobacterium animalis subspecies lactis HN019, Lactobacillus fermentum CECT5716 and Lactobacillus rhamnosus HN001, and can be a solid powder. The sieve mesh number of the solid probiotic powder is 50-70 meshes.

[0033] The hawthorn and chicken's gizzard lining are sieved with a mesh number of 80-100, the tuckahoe and malt are sieved with a mesh number of 120-150, and the solid probiotic powder is sieved with a mesh number of 50-70. The present invention has found through research that by controlling the dosage ratio and particle size of the above-mentioned medicinal and edible raw materials and probiotic powder, it is helpful to improve its storage stability, so that the solid composition is not easy to stick together, and it is also helpful to form a fine, non-granular, more evenly dispersed and better-tasting suspension when re-dissolving with water.

[0034] The inventors found in the study that the solid composition obtained by using the medicinal and edible raw materials and probiotics of the present invention together with foaming agents, sweeteners, acidity regulators, etc. is easy to agglomerate, and the product quality and taste are poor. Therefore, the above technical effects can be achieved through the mutual cooperation between the medicinal and edible raw materials and probiotics. At the same time, through the compatibility between hawthorn, chicken gizzard, Poria cocos, and malt in the raw materials, the dissolution of each effective ingredient is promoted, and the fermentation process of specific probiotics is coordinated, which is conducive to the colonization of probiotics in the intestines, and plays a certain degree of gastrointestinal conditioning, promoting intestinal peristalsis, moistening the intestines and laxative, and regulating immunity.

[0035] In another specific embodiment of the present invention, there is provided the use of the probiotic-containing composition in the preparation of products for regulating gastrointestinal function, moistening the intestines and promoting bowel movements, and improving immunity.

[0036] The product may be food or medicine, and the food may be health food, specifically a solid beverage, which is not specifically limited here.

[0037] The present invention is further explained by the following examples, but it does not constitute a limitation of the present invention. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. In the present invention, each raw material component is obtained by commercial means.

[0038] Example 1 A composition containing probiotics, comprising the following raw materials: 10 parts of hawthorn, 1 part of chicken gizzard lining, 2 parts of poria, 3 parts of malt, 0.5 parts of rhamnosus lactobacillus GG, 0.5 parts of animal bifidobacterium lactis subsp. HN019, 0.05 parts of fermented lactobacillus CECT5716, and 0.05 parts of rhamnosus lactobacillus HN001; wherein the ratio of the live counts of rhamnosus lactobacillus GG, animal bifidobacterium lactis subsp. HN019, fermented lactobacillus CECT5716, and rhamnosus lactobacillus HN001 is 50:50:1:1; and the total live count of the probiotics is ≥10 billion cfu / g.

[0039] The preparation method comprises: crushing and mixing hawthorn, chicken's gizzard lining, poria cocos and malt respectively to obtain a mixed raw material; the hawthorn and chicken's gizzard lining are crushed and sieved to a mesh number of 90 meshes, and the poria cocos and malt are crushed and sieved to a mesh number of 120 meshes.

[0040] The probiotics are added to the mixed raw materials, mixed and packaged. Each bag of the composition containing probiotics is 1g. The probiotics are a mixed solid powder of the above four probiotics, and the solid probiotic powder is crushed and sieved with a mesh number of 60 meshes.

[0041] Example 2 A composition containing probiotics, comprising the following raw materials: 10 parts of hawthorn, 0.5 parts of chicken gizzard lining, 3 parts of poria, 2 parts of malt, 0.5 parts of rhamnosus lactobacillus GG, 0.5 parts of animal bifidobacterium lactis subsp. HN019, 0.05 parts of fermented lactobacillus CECT5716, and 0.05 parts of rhamnosus lactobacillus HN001; wherein the ratio of the live counts of rhamnosus lactobacillus GG, animal bifidobacterium lactis subsp. HN019, fermented lactobacillus CECT5716, and rhamnosus lactobacillus HN001 is 50:50:1:1; and the total live count of the probiotics is ≥10 billion cfu / g.

[0042] The preparation method comprises: crushing and mixing hawthorn, chicken's gizzard lining, poria cocos and malt respectively to obtain a mixed raw material; the hawthorn and chicken's gizzard lining are crushed and sieved to a mesh number of 80 meshes, and the poria cocos and malt are crushed and sieved to a mesh number of 140 meshes.

[0043] The probiotics are added to the mixed raw materials, mixed and packaged. Each bag of the composition containing probiotics is 1g. The probiotics are a mixed solid powder of the above four probiotics, and the solid probiotic powder is crushed and sieved with a mesh number of 50 meshes.

[0044] Example 3 A composition containing probiotics, comprising the following raw materials: 18 parts of hawthorn, 2 parts of chicken gizzard lining, 1 part of poria, 5 parts of malt, 0.5 parts of rhamnosus lactobacillus GG, 0.5 parts of animal bifidobacterium lactis subsp. HN019, 0.05 parts of fermented lactobacillus CECT5716, and 0.05 parts of rhamnosus lactobacillus HN001; wherein the ratio of the live counts of rhamnosus lactobacillus GG, animal bifidobacterium lactis subsp. HN019, fermented lactobacillus CECT5716, and rhamnosus lactobacillus HN001 is 50:50:1:1; and the total live count of the probiotics is ≥10 billion cfu / g.

[0045] The preparation method comprises: crushing and mixing hawthorn, chicken's gizzard lining, poria cocos and malt respectively to obtain a mixed raw material; the hawthorn and chicken's gizzard lining are crushed and sieved with a mesh number of 100 meshes, and the poria cocos and malt are crushed and sieved with a mesh number of 150 meshes.

[0046] The probiotics are added to the mixed raw materials, mixed and packaged. Each bag of the composition containing probiotics is 1g. The probiotics are a mixed solid powder of the above four probiotics, and the solid probiotic powder is crushed and sieved with a mesh number of 70 meshes.

[0047] Comparative Example 1 The preparation method is the same as that in Example 1, except that the mesh number of each raw material sieved in Example 1 is 100 mesh.

[0048] Comparative Example 2 The preparation method is the same as that in Example 1, except that the hawthorn and chicken's gizzard lining are crushed and sieved with a mesh number of 120 meshes, and the poria and malt are sieved with a mesh number of 90 meshes.

[0049] Comparative Example 3 The preparation method is the same as that of Example 1, except that the solid probiotic powder is sieved with 90 meshes.

[0050] Comparative Example 4 The preparation method is the same as that of Example 1, except that malt is replaced with an equal amount of coix seeds.

[0051] Comparative Example 5 The preparation method is the same as that of Example 1, except that hawthorn is replaced with an equal amount of tangerine peel.

[0052] Effect verification (1) Storage stability verification The samples prepared in the above examples and comparative examples were subjected to accelerated tests (temperature 35° C., relative humidity 75±5%), and the results were recorded as shown in Table 1.

[0053] Table 1 Accelerated test results

[0054] It can be seen from Table 1 above that the selection of raw materials and particle size have a great influence on the storage stability of the final solid composition, and the occurrence of agglomeration has a chain reaction on the number of viable bacteria, that is, the more serious the agglomeration phenomenon is, the greater the decrease in the number of viable bacteria.

[0055] (2) Improve the evaluation of immune function Thirty Kunming mice (half male and half female, weighing 18-22 g) were divided into a control group, a model group and an experimental group, with 10 mice randomly assigned to each group. Among them, the control group and the model group were given 0.5 mL sterile water for gavage once a day; the experimental group was given a solution of a probiotic product (50 mg dissolved in 0.5 mL sterile water, Example 1) for gavage once a day for 14 consecutive days. At the same time, the model group and the experimental group were intraperitoneally injected with cyclophosphamide (30 mg / kg, bw) three times a day on the first, third and fifth days, respectively, and the control group was injected with the same volume of saline. After 14 consecutive days of intervention, mice in each group were fasted but not watered for 16 hours. The thymus and spleen were dissected and the organ index was calculated. Organ index = [average organ weight (mg) / average body weight (g)]. The specific results are shown in Table 2 below.

[0056] Table 2 Effect of the composition on improving immune function

[0057] As shown in Table 2, compared with the model group, the thymus index and spleen index of the experimental group were significantly improved, and even equal to or slightly higher than that of the control group, indicating that the above-mentioned composition containing probiotics can enhance the immunity of mice.

[0058] (3) Evaluation of laxative function Thirty Kunming mice (half male and half female, weighing 18-22 g) were divided into a control group, a model group, and an experimental group, with 10 mice randomly assigned to each group. The control group and the model group were given 0.5 mL of sterile water by gavage once a day; the experimental group was given a solution of a probiotic product (50 mg dissolved in 0.5 mL of sterile water, Example 1) by gavage once a day. After 14 consecutive days of intervention, the mice in each group were fasted but not watered for 16 hours.

[0059] The model group and the experimental group were given compound diphenoxylate (5 mg / kg, bw) by gavage, and the control group was given the same volume of distilled water by gavage. After 30 minutes, mice in each group were given gum arabic ink (containing 10% activated carbon powder and 10% activated gum arabic) by gavage. After 30 minutes, 5 mice were randomly selected from each group and killed by cervical dislocation. The intestine from the pylorus to the cecum was quickly removed. The intestinal length was measured as the total length of the small intestine L0 (cm), and the length from the pylorus to the front of the ink was the ink advancement length L1 (cm). The calculation formula for the small intestine ink advancement rate is as follows: ink advancement rate = L1 / L0×100%. The remaining 5 mice in each group were given ink by gavage, and their first black stool time, the number of stool particles in 6 hours, and the total mass of feces were recorded. The specific results are shown in Table 3.

[0060] Table 3 Laxative function of the composition

[0061] From the above results, it can be seen that the composition containing probiotics prepared by the present invention can significantly improve the intestinal ink propulsion rate, significantly shorten the time of first black stool discharge, and significantly increase the number of black stool particles discharged in 6 hours and the weight of black stool discharged in 6 hours. It shows that the composition of the present application has a good effect of promoting intestinal peristalsis and moistening the intestines and relieving constipation.

[0062] (4) Small-scale population verification 20 participants who work in a sedentary environment and report constipation were selected to take the composition containing probiotics (Example 1) orally, once a day, 2 g each time, and prepared by adding 100 ml of warm water (35°C). When re-dissolved in water, a suspension that is evenly dispersed, fine, has no granularity, and has a good taste can be formed. After taking it for 1 month, a feedback survey was conducted on each participant, and the evaluation criteria were as follows: Significant effect: Constipation is significantly improved, the interval time and stool quality are close to normal, or the stool is slightly dry and the interval time is within 72 hours, and most other symptoms disappear; Effective: The interval between bowel movements is shortened by 1 day, or the stool quality is improved, and other symptoms are improved; Ineffective: No improvement in constipation or other symptoms.

[0063] According to statistics, 10 people reported marked effects and 6 people reported effective results, that is, the total number of marked and effective results was 16 people, accounting for 80%, indicating that the solid composition of the present invention has the effect of promoting intestinal peristalsis and moistening the intestines and relieving constipation.

[0064] Matters not covered by the present invention are known technologies.

[0065] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition containing probiotics, characterized in that: The invention comprises the following raw materials in parts by weight: 3-20 parts of hawthorn, 0.1-2 parts of chicken's gizzard lining, 0.3-3 parts of poria, 0.5-5 parts of malt, 0.2-2 parts of rhamnosus lactobacillus GG, 0.2-2 parts of animal Bifidobacterium lactis subspecies HN019, 0.02-1 parts of fermented Lactobacillus CECT5716, and 0.02-1 parts of rhamnosus lactobacillus HN001.

2. The composition containing probiotics according to claim 1, characterized in that The raw material components include: 10 parts of hawthorn, 1 part of chicken gizzard lining, 2 parts of poria, 3 parts of malt, 0.5 parts of rhamnosus lactobacillus GG, 0.5 parts of animal bifidobacterium lactis subspecies HN019, 0.05 parts of fermented lactobacillus CECT5716 and 0.05 parts of rhamnosus lactobacillus HN001.

3. The composition containing probiotics according to claim 1 or 2, characterized in that The ratio of the live bacterial counts of Lactobacillus rhamnosus GG, Bifidobacterium animalis subspecies lactis HN019, Lactobacillus fermentum CECT5716 and Lactobacillus rhamnosus HN001 is 50:50:0.5-5:0.5-5.

4. The composition containing probiotics according to claim 3, characterized in that The total number of viable probiotics is 10-20 billion cfu / g.

5. The method for preparing the probiotic composition according to any one of claims 1 to 4, characterized in that: The method comprises the following steps: crushing hawthorn, chicken gizzard lining, poria cocos and malt respectively, and mixing them evenly to obtain a mixed raw material; adding probiotics into the mixed raw material, and mixing and packaging the mixed raw material.

6. The preparation method according to claim 5, characterized in that: The probiotics are a mixed solid powder of Lactobacillus rhamnosus GG, Bifidobacterium animalis lactis subspecies HN019, Lactobacillus fermentum CECT5716 and Lactobacillus rhamnosus HN001, and the sieve mesh number of the solid probiotic powder is 50-70 meshes.

7. The preparation method according to claim 5, characterized in that: The sieve mesh number of the hawthorn and chicken's gizzard lining is 80-100 meshes.

8. The preparation method according to claim 5, characterized in that: The sieving mesh number of the Poria cocos and malt is 120-150 meshes.

9. Use of the probiotic composition according to any one of claims 1 to 4 in the preparation of products for regulating gastrointestinal function, moistening the intestines and promoting bowel movements, and improving immunity.

10. The use according to claim 9, characterized in that: The product is a food or a drug.

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