Process for promoting growth of commercial crops by using lincomycin finished product liquid fertilizer
Through the lincomycin finished liquid liquid fertilizer process, the problem of inconsistent nitrogen, phosphorus and potassium ratio in liquid fertilizer is solved, and the growth and photosynthesis efficiency of plants is improved, and high yield and high quality crop growth is achieved.
Patent Information
- Application Number
- CN202411891374.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-02-28
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The current liquid fertilizer does not meet the requirements of nitrogen, phosphorus and potassium during the plant flowering period or lacks trace elements, resulting in insufficient or excessive nutrient supply, affecting the photosynthesis and flowering performance of plants.
The liquid fertilizer containing appropriate amounts of nitrogen, phosphorus, potassium and trace elements were prepared by diluting the dry powder of lincomycin, adjusting the concentration of ammonium ion in the fermentation medium, and adding modification accelerators and other nutrients.
It improves the growth rate, photosynthesis efficiency and stress resistance of plants, ensures that plants obtain sufficient nutrients during the flowering period, and promotes high yield and quality of crops.
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Figure SMS_1
Abstract
Description
Technical Field
[0001] The invention belongs to the field of liquid fertilizers, in particular to a process for promoting the growth of economic crops by using lincomycin finished liquid fertilizer. Background Art
[0002] Liquid compound fertilizer (Fluid Fertilizer), also known as fluid compound fertilizer, commonly known as liquid fertilizer, is a liquid product containing two or more nutrients required by crops. These nutrients are dissolved in water as solutes to form a solution or suspended in water with the help of a suspending agent to form a suspended paste. Liquid fertilizers have been developed to date and there are many varieties, which can be roughly divided into two categories: liquid nitrogen fertilizer and liquid compound fertilizer. Among them, the effective substances of liquid nitrogen fertilizer include ammonium, nitrate and amide nitrogen, such as liquid ammonia, ammonia water, and nitrogen solution. Liquid compound fertilizer contains all the nutrients required for plant growth, such as nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and trace elements, and can also be added with organic substances soluble in water (such as humic acid, amino acids, plant growth regulators, etc.). Liquid compound fertilizer can be designed according to the nutritional requirements of crop growth. The fertilizer formula can be adjusted at any time according to the different growth of crops. Scientific formulas can significantly improve fertilizer utilization. The fertilizer efficiency of liquid fertilizers is usually more than 30% higher than that of conventional compound fertilizers.
[0003] Lincomycin acts on the 50S subunit of the ribosome of sensitive bacteria, inhibiting the protein synthesis of bacterial cells by preventing the extension of the peptide chain. It is generally an antibacterial agent, but at high concentrations, it also has a bactericidal effect on highly sensitive bacteria.
[0004] In the prior art, liquid fertilizer has the following problems: The ratio of nitrogen, phosphorus and potassium required by plants during flowering is different from that during growth stages. If the ratio of nitrogen, phosphorus and potassium in liquid fertilizer does not meet the needs of plants during flowering, it may lead to insufficient or excessive nutrient supply.
[0005] In addition to nitrogen, phosphorus and potassium, plants also need a variety of trace elements to maintain normal growth and flowering. If these trace elements are lacking in liquid fertilizer, it may lead to poor performance of the plant during flowering. Summary of the invention
[0006] In order to make up for the deficiencies of the prior art and solve at least one technical problem raised in the background technology, for example, if the content of one or more nutrient elements in the compound liquid fertilizer is insufficient or excessive after use, it may cause the problem that crop photosynthesis is hindered.
[0007] The technical solution adopted by the present invention to solve the technical problem is: the process of promoting the growth of economic crops with a lincomycin finished liquid fertilizer of the present invention comprises the following steps: Step 1: diluting lincomycin dry powder at a mass ratio of 1:100 to obtain a lincomycin finished liquid; Step 2: aeration culture in a liquid culture medium containing starch, yeast powder, soybean powder, yeast extract and inorganic salts, during the lincomycin fermentation process, adjusting the ammonium ion concentration in the initial fermentation medium to 57±5mmol / L, and culturing lincomycin Streptomyces under suitable conditions; when the ammonium ion concentration in the fermentation medium is 0.2mmol / L, adding ammonium ions, and then controlling the ammonium ion concentration in the fermentation medium to 2mmol / L until the fermentation is completed, and the fermentation time is controlled to be 36-110h, to obtain a lincomycin fermentation liquid; Step 3: modified accelerator 65-75, urea 8-12, potassium dihydrogen phosphate 5-7, superphosphate 2-8, calcium magnesium phosphate fertilizer 2-8, potassium chloride 3-4, zinc sulfate 0.2-0.3, borax 0.3-0.4, fulvic acid iron solution 0.15-0.2, paclobutrazol 0.005-0.01, lincomycin fermentation liquid 10-100; Dissolve urea, potassium chloride, zinc sulfate and ferric humate solution in water and mix them evenly to prepare a nutrient solution; After 200 mesh zeolite is subjected to a high temperature treatment at 200°C, the above nutrient solution is sprayed onto the zeolite; Drying the zeolite in the sun or by drying it to reduce its moisture content to no more than 5%; The remaining chemical fertilizer is then evenly mixed with the modified accelerator to form lincomycin finished liquid fertilizer.
[0008] As a technical solution of the present invention: at the 20th to 24th hour of cultivation, when amino nitrogen [NH2-N] ≤ 0.35 g / L, 1.067 g / L of ammonium sulfate is added.
[0009] As a technical solution of the present invention: during the 24th to 40th hour of cultivation, when the amino nitrogen is ≤0.55 g / L, 0.8 g / L of ammonium sulfate is added.
[0010] As a technical solution of the present invention: during the 40th to 140th hour of cultivation, when the amino nitrogen is ≤0.55 g / L, 0.533 g / L of ammonium sulfate is added.
[0011] As a technical solution of the present invention: from the 140th hour of cultivation to the time of tank release, when the amino nitrogen is ≤0.55 g / L, 0.267 g / L of ammonium sulfate is added.
[0012] As a technical solution of the present invention: the preparation process of the modified accelerator comprises the following steps: It is prepared from polylactic acid-glycolic acid copolymer and 6-benzylaminopurine in a mass ratio of 1:0.5-1; the sucrose ester biosurfactant is prepared from sucrose and esterifying agent palmitic acid under the catalysis of lipase.
[0013] As a technical solution of the present invention, the following steps are: inoculating Paecilomyces cicadae onto a slant culture medium, and culturing the culture medium at a constant temperature of 25-30° C. for 5-7 days for later use; Inoculate Paecilomyces cicadae into a 150 mL Erlenmeyer flask containing 50-55 mL of liquid culture medium, and culture on a shaker at 140-150 r / min and 25-30° C. for 72 h to 90 h; The seed-cultured Paecilomyces cicadae liquid was inoculated into a 500 mL Erlenmeyer flask containing 150 mL of liquid fermentation medium at an inoculation rate of 5-9%, and cultured on a shaking table at 150-160 r / min and 25-30° C. for 84 h to 96 h.
[0014] As a technical solution of the present invention: the components of the slant culture medium are 95-100 parts by weight of potato rhizome extract, 10-15 parts by weight of glucose, 15-20 parts by weight of agar, 500 mL of distilled water, and the pH is adjusted to 6.0-7.0.
[0015] As a technical solution of the present invention: the components of the liquid culture medium are 10-13 parts by weight of glucose, 5-7 parts by weight of peptone, 4-6 parts by weight of yeast extract, 0.7-0.9 parts by weight of potassium dihydrogen phosphate, and 0.5-1 parts by weight of magnesium sulfate, distilled water is added to 1000 mL, and the pH is adjusted to 6.5-7.0.
[0016] As the technical scheme of the present invention: the preparation steps of liquid fermentation medium are as follows: 1500mL of distilled water is added to the medium, and honey, citrus juice, glucose, peptone, yeast extract, soybean oil, potassium dihydrogen phosphate and magnesium sulfate are added in sequence, and stirred continuously until completely dissolved, the pH is adjusted to 6.8-7.2, and finally a modified growth promoter and a sucrose ester biosurfactant are added to obtain a liquid fermentation culture of Pseudomonas cicadae.
[0017] The beneficial effects of the present invention are as follows: The present invention dilutes lincomycin dry powder at a mass ratio of 1:100 to obtain a lincomycin finished liquid; Step 2: aeration culture in a liquid culture medium containing starch, yeast powder, soybean powder, yeast extract and inorganic salts, during the lincomycin fermentation process, adjusting the ammonium ion concentration in the initial fermentation medium to 57±5mmol / L, and culturing lincomycin Streptomyces under suitable conditions; when the ammonium ion concentration in the fermentation medium is 0.2mmol / L, adding ammonium ions, and then controlling the ammonium ion concentration in the fermentation medium to 2mmol / L until the fermentation is completed, and the fermentation time is controlled to be 36-110h, to obtain a lincomycin fermentation liquid; Step 3: modified accelerator 65-75, urea 8-12, potassium dihydrogen phosphate 5-7, superphosphate 2-8, calcium magnesium phosphate fertilizer 2-8, potassium chloride 3-4, zinc sulfate 0.2-0.3, borax 0.3-0.4, fulvic acid iron solution 0.15-0.2, paclobutrazol 0.005-0.01, lincomycin fermentation liquid 10-100; Dissolve urea, potassium chloride, zinc sulfate and ferric humate solution in water and mix them evenly to prepare a nutrient solution; After 200 mesh zeolite is subjected to a high temperature treatment at 200°C, the above nutrient solution is sprayed onto the zeolite; Drying the zeolite in the sun or by drying it to reduce its moisture content to no more than 5%; Then the remaining chemical fertilizer is evenly mixed with the modified accelerator to obtain the lincomycin finished liquid fertilizer; Among them, the role of modified accelerator: 1. Promote cell division and differentiation Plant growth promoters can stimulate plant cell division and differentiation, thereby accelerating plant growth. For example, chlorfenapyr is a highly active compound with cytokinin activity that can promote cell division and expansion. When applied to melon and fruit plants, it can promote flower bud differentiation, preserve flowers and fruits, increase fruit setting rate, and promote fruit enlargement.
[0018] 2. Enhance photosynthesis and metabolism These accelerators also improve the efficiency of photosynthesis in plants and promote metabolic processes in plants. By enhancing the synthesis and activity of chlorophyll, plants are better able to use sunlight for photosynthesis, thereby producing more energy and nutrients, supporting rapid growth and development of plants.
[0019] 3. Promote the development of reproductive organs Plant growth promoters also significantly promote the development of reproductive organs of plants. They can stimulate the formation and differentiation of flower buds, increase the quantity and quality of flower buds, and thus promote early flowering and fruiting of plants. This is of great significance for improving the yield and quality of crops.
[0020] 4. Improve stress resistance By regulating the physiological mechanism in the plant, plant growth promoters can also improve the stress resistance of plants. They can help plants better adapt to adverse environmental conditions, such as drought, cold, salinity, etc., thereby improving the survival ability and yield of plants. For example, plant growth substances such as DA-6 can enhance the absorption of water and fertilizer by plants and the accumulation of dry matter, regulate the water balance in the body, and enhance the disease resistance, drought resistance, and cold resistance of crops and fruit trees. Among them, the lincomycin finished liquid fertilizer of the present invention improves the growth of crops. DETAILED DESCRIPTION
[0021] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.
[0022] Embodiment 1 The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to an embodiment of the present invention comprises the following steps: Step 1: diluting lincomycin dry powder at a mass ratio of 1:100 to obtain a lincomycin finished liquid; Step 2: aeration culture in a liquid culture medium containing starch, yeast powder, soybean powder, yeast extract and inorganic salts, during the lincomycin fermentation process, adjusting the ammonium ion concentration in the initial fermentation medium to 57±5mmol / L, and culturing lincomycin Streptomyces under suitable conditions; when the ammonium ion concentration in the fermentation medium is 0.2mmol / L, adding ammonium ions, and then controlling the ammonium ion concentration in the fermentation medium to 2mmol / L until the fermentation is completed, and the fermentation time is controlled to be 36-110h, to obtain a lincomycin fermentation liquid; Among them, the ammonium ion concentration in the initial fermentation medium is adjusted to 57±1mmol / L; at the 20th to 24th hour of cultivation, when the amino nitrogen [NH2-N] is ≤0.35g / L, 1.067g / L of ammonium sulfate is added; at the 24th to 40th hour of cultivation, when the amino nitrogen is ≤0.55g / L, 0.8g / L of ammonium sulfate is added; at the 40th to 140th hour of cultivation, when the amino nitrogen is ≤0.55g / L, 0.533g / L of ammonium sulfate is added; at the 140th hour of cultivation until the tank is released, when the amino nitrogen is ≤0.55g / L, 0.267g / L of ammonium sulfate is added; Step 3: The following components in parts by weight: 65 parts of a modified accelerator, 8 parts of urea, 5 parts of potassium dihydrogen phosphate, 2 parts of superphosphate, 2 parts of calcium magnesium phosphate fertilizer, 3 parts of potassium chloride, 0.2 parts of zinc sulfate, 0.3 parts of borax, 0.15 parts of ferric fulvic acid solution, 0.005 parts of paclobutrazol, and 10 parts of lincomycin fermentation liquid; Dissolve urea, potassium chloride, zinc sulfate and ferric humate solution in water and mix them evenly to prepare a nutrient solution; After 200 mesh zeolite is subjected to a high temperature treatment at 200°C, the above nutrient solution is sprayed onto the zeolite; Drying the zeolite in the sun or by drying it to reduce its moisture content to no more than 5%; The remaining chemical fertilizer is then mixed evenly with the zeolite to form a lincomycin finished liquid fertilizer, which contains 3.7% nitrogen, 3.3% phosphorus pentoxide, and 3.2% potassium oxide; The preparation process of the modified accelerator comprises the following steps: It is prepared from polylactic acid-glycolic acid copolymer and 6-benzylaminopurine in a mass ratio of 1:0.5-1; the sucrose ester biosurfactant is prepared from sucrose and esterifying agent palmitic acid under the catalysis of lipase; Inoculate Paecilomyces cicadae on the slant culture medium and culture at a constant temperature of 25-30°C for 5-7 days for later use; Inoculate Paecilomyces cicadae into a 150 mL Erlenmeyer flask containing 50-55 mL of liquid culture medium, and culture on a shaker at 140-150 r / min and 25-30° C. for 72 h to 90 h; The seed-cultured Paecilomyces cicadae liquid was inoculated into a 500 mL Erlenmeyer flask containing 150 mL of liquid fermentation medium at an inoculation rate of 5-9%, and cultured on a shaker at 150 r / min and 25-30° C. for 84 h to 96 h; In S1.2, the components of the slant culture medium are 95-100 parts by weight of potato rhizome extract, 10 parts by weight of glucose, 15 parts by weight of agar, 500 mL of distilled water, and the pH is adjusted to 6.0; In S1.3, the components of the liquid culture medium are 10 parts by weight of glucose, 5 parts by weight of peptone, 4 parts by weight of yeast extract, 0.7 parts by weight of potassium dihydrogen phosphate, and 0.5 parts by weight of magnesium sulfate. Distilled water is added to 1000 mL, and the pH is adjusted to 6.5. In the S1.4, the preparation step of the liquid fermentation medium is as follows: 1500 mL of distilled water is added to the culture medium, and honey, citrus juice, glucose, peptone, yeast extract, soybean oil, potassium dihydrogen phosphate and magnesium sulfate are added in sequence, and stirred continuously until completely dissolved, the pH is adjusted to 6.8, and finally a modified growth promoter and a sucrose ester biosurfactant are added to obtain a liquid fermentation culture of Pseudomonas cicadae.
[0023] Embodiment 2 The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to an embodiment of the present invention comprises the following steps: Step 1: diluting lincomycin dry powder at a mass ratio of 1:100 to obtain a lincomycin finished liquid; Step 2: aeration culture in a liquid culture medium containing starch, yeast powder, soybean powder, yeast extract and inorganic salts, during the lincomycin fermentation process, adjusting the ammonium ion concentration in the initial fermentation medium to 57±5mmol / L, and culturing lincomycin Streptomyces under suitable conditions; when the ammonium ion concentration in the fermentation medium is 0.2mmol / L, adding ammonium ions, and then controlling the ammonium ion concentration in the fermentation medium to 2mmol / L until the fermentation is completed, and the fermentation time is controlled to be 36-110h, to obtain a lincomycin fermentation liquid; Among them, the ammonium ion concentration in the initial fermentation medium is adjusted to 57±1mmol / L; at the 20th to 24th hour of cultivation, when the amino nitrogen [NH2-N] is ≤0.35g / L, 1.067g / L of ammonium sulfate is added; at the 24th to 40th hour of cultivation, when the amino nitrogen is ≤0.55g / L, 0.8g / L of ammonium sulfate is added; at the 40th to 140th hour of cultivation, when the amino nitrogen is ≤0.55g / L, 0.533g / L of ammonium sulfate is added; at the 140th hour of cultivation until the tank is released, when the amino nitrogen is ≤0.55g / L, 0.267g / L of ammonium sulfate is added; Step 3: The following components in parts by weight: 65 parts of a modified accelerator, 8 parts of urea, 5 parts of potassium dihydrogen phosphate, 2 parts of superphosphate, 2 parts of calcium magnesium phosphate fertilizer, 3 parts of potassium chloride, 0.2 parts of zinc sulfate, 0.3 parts of borax, 0.15 parts of ferric fulvic acid solution, 0.005 parts of paclobutrazol, and 10 parts of lincomycin fermentation liquid; Dissolve urea, potassium chloride, zinc sulfate and ferric humate solution in water and mix them evenly to prepare a nutrient solution; After 200 mesh zeolite is subjected to a high temperature treatment at 200°C, the above nutrient solution is sprayed onto the zeolite; Drying the zeolite in the sun or by drying it to reduce its moisture content to no more than 5%; The remaining chemical fertilizer is then mixed evenly with the zeolite to form a lincomycin finished liquid fertilizer, which contains 3.7% nitrogen, 3.3% phosphorus pentoxide, and 3.2% potassium oxide; The preparation process of the modified accelerator comprises the following steps: It is prepared from polylactic acid-glycolic acid copolymer and 6-benzylaminopurine in a mass ratio of 1:0.8; the sucrose ester biosurfactant is prepared from sucrose and esterifying agent palmitic acid under the catalysis of lipase; Inoculate Paecilomyces cicadae on the slant culture medium and culture at a constant temperature of 25-30°C for 5-7 days for later use; Inoculate Paecilomyces cicadae into a 150 mL Erlenmeyer flask containing 50-55 mL of liquid culture medium, and culture on a shaker at 140-150 r / min and 25-30° C. for 72 h to 90 h; The seed-cultured Paecilomyces cicadae liquid was inoculated into a 500 mL Erlenmeyer flask containing 150 mL of liquid fermentation medium at an inoculation rate of 7%, and cultured on a shaker at 150-160 r / min and 25-30° C. for 84-96 hours; In S1.2, the components of the slant culture medium are 98 parts by weight of potato rhizome extract, 12 parts by weight of glucose, 18 parts by weight of agar, and 500 mL of distilled water, and the pH is adjusted to 6.0-7.0; In S1.3, the components of the liquid culture medium are 12 parts by weight of glucose, 6 parts by weight of peptone, 5 parts by weight of yeast extract, 0.8 parts by weight of potassium dihydrogen phosphate, and 0.7 parts by weight of magnesium sulfate. Distilled water is added to 1000 mL, and the pH is adjusted to 6.5-7.0; In the S1.4, the preparation step of the liquid fermentation medium is as follows: 1500 mL of distilled water is added to the culture medium, and honey, citrus juice, glucose, peptone, yeast extract, soybean oil, potassium dihydrogen phosphate and magnesium sulfate are added in sequence, and stirred continuously until completely dissolved, the pH is adjusted to 6.8-7.2, and finally a modified growth promoter and a sucrose ester biosurfactant are added to obtain a liquid fermentation culture of Pseudomonas cicadae.
[0024] Embodiment 3 The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to an embodiment of the present invention comprises the following steps: Step 1: diluting lincomycin dry powder at a mass ratio of 1:100 to obtain a lincomycin finished liquid; Step 2: aeration culture in a liquid culture medium containing starch, yeast powder, soybean powder, yeast extract and inorganic salts, during the lincomycin fermentation process, adjusting the ammonium ion concentration in the initial fermentation medium to 57±5mmol / L, and culturing lincomycin Streptomyces under suitable conditions; when the ammonium ion concentration in the fermentation medium is 0.2mmol / L, adding ammonium ions, and then controlling the ammonium ion concentration in the fermentation medium to 2mmol / L until the fermentation is completed, and the fermentation time is controlled to be 36-110h, to obtain a lincomycin fermentation liquid; Among them, the ammonium ion concentration in the initial fermentation medium is adjusted to 57±1mmol / L; at the 20th to 24th hour of cultivation, when the amino nitrogen [NH2-N] is ≤0.35g / L, 1.067g / L of ammonium sulfate is added; at the 24th to 40th hour of cultivation, when the amino nitrogen is ≤0.55g / L, 0.8g / L of ammonium sulfate is added; at the 40th to 140th hour of cultivation, when the amino nitrogen is ≤0.55g / L, 0.533g / L of ammonium sulfate is added; at the 140th hour of cultivation until the tank is released, when the amino nitrogen is ≤0.55g / L, 0.267g / L of ammonium sulfate is added; Step 3: The following components in parts by weight: 65 parts of a modified accelerator, 8 parts of urea, 5 parts of potassium dihydrogen phosphate, 2 parts of superphosphate, 2 parts of calcium magnesium phosphate fertilizer, 3 parts of potassium chloride, 0.2 parts of zinc sulfate, 0.3 parts of borax, 0.15 parts of ferric fulvic acid solution, 0.005 parts of paclobutrazol, and 10 parts of lincomycin fermentation liquid; Dissolve urea, potassium chloride, zinc sulfate and ferric humate solution in water and mix them evenly to prepare a nutrient solution; After 200 mesh zeolite is subjected to a high temperature treatment at 200°C, the above nutrient solution is sprayed onto the zeolite; Drying the zeolite in the sun or by drying it to reduce its moisture content to no more than 5%; The remaining chemical fertilizer is then mixed evenly with the zeolite to form a lincomycin finished liquid fertilizer, which contains 3.7% nitrogen, 3.3% phosphorus pentoxide, and 3.2% potassium oxide; The preparation process of the modified accelerator comprises the following steps: It is prepared from polylactic acid-glycolic acid copolymer and 6-benzylaminopurine in a mass ratio of 1:1; sucrose ester biosurfactant is prepared from sucrose and esterifying agent palmitic acid under the catalysis of lipase; Inoculate Paecilomyces cicadae on the slant culture medium and culture at a constant temperature of 25-30°C for 5-7 days for later use; Inoculate Paecilomyces cicadae into a 150 mL Erlenmeyer flask containing 55 mL of liquid culture medium, and culture on a shaker at 140-150 r / min and 25-30° C. for 72 h to 90 h; The seed-cultured Paecilomyces cicadae liquid was inoculated into a 500 mL Erlenmeyer flask containing 150 mL of liquid fermentation medium at an inoculation rate of 9%, and cultured on a shaking table at 150-160 r / min and 25-30° C. for 84 h to 96 h; In S1.2, the components of the slant culture medium are 100 parts by weight of potato rhizome extract, 15 parts by weight of glucose, 20 parts by weight of agar, 500 mL of distilled water, and the pH is adjusted to 7.0; In S1.3, the components of the liquid culture medium are 13 parts by weight of glucose, 7 parts by weight of peptone, 6 parts by weight of yeast extract, 0.9 parts by weight of potassium dihydrogen phosphate, and 1 part by weight of magnesium sulfate. Distilled water is added to 1000 mL, and the pH is adjusted to 7.0. In the S1.4, the preparation step of the liquid fermentation medium is as follows: 1500 mL of distilled water is added to the culture medium, and honey, citrus juice, glucose, peptone, yeast extract, soybean oil, potassium dihydrogen phosphate and magnesium sulfate are added in sequence, and stirred continuously until completely dissolved, the pH is adjusted to 6.8-7.2, and finally a modified growth promoter and a sucrose ester biosurfactant are added to obtain a liquid fermentation culture of Pseudomonas cicadae.
[0025] Comparative Example 1 Comparative Example 1 adopts the preparation process of the liquid fertilizer, comprising the following steps: The invention is composed of the following components in parts by weight: 12 parts of urea, 7 parts of potassium dihydrogen phosphate, 8 parts of superphosphate, 8 parts of calcium magnesium phosphate fertilizer, 4 parts of potassium chloride, 0.3 parts of zinc sulfate, 0.4 parts of borax, 0.2 parts of fulvic acid iron solution, and 0.01 parts of paclobutrazol; Dissolve urea, potassium chloride, zinc sulfate and ferric humate solution in water and mix them evenly to prepare a nutrient solution; After 200 mesh zeolite is subjected to a high temperature treatment at 200°C, the above nutrient solution is sprayed onto the zeolite; Drying the zeolite in the sun or by drying it to reduce its moisture content to no more than 5%; Then mix the remaining chemical fertilizer with zeolite to obtain liquid fertilizer.
[0026] Performance Test: For the composite liquid fertilizers prepared in Examples 1-3 and Comparative Example 1, the field test was conducted on corn cultivation. Based on the plant height and root length of corn, the effects of different tyvalosin finished liquid fertilizers on corn production were obtained, and Table 1 was obtained:
[0027] It can be seen from the above table that the lincomycin finished liquid fertilizer of the present invention improves the growth of crops.
[0028] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.
Claims
1. A process for promoting the growth of cash crops using lincomycin finished liquid fertilizer, characterized in that: The following steps are involved: Step 1: diluting lincomycin dry powder at a mass ratio of 1:100 to obtain a lincomycin finished liquid; Step 2: aeration culture in a liquid culture medium containing starch, yeast powder, soybean powder, yeast extract and inorganic salts, during the lincomycin fermentation process, adjusting the ammonium ion concentration in the initial fermentation medium to 57±5mmol / L, and culturing lincomycin Streptomyces under suitable conditions; when the ammonium ion concentration in the fermentation medium is 0.2mmol / L, adding ammonium ions, and then controlling the ammonium ion concentration in the fermentation medium to 2mmol / L until the fermentation is completed, and the fermentation time is controlled to be 36-110h, to obtain a lincomycin fermentation liquid; Step 3: modified accelerator 65-75, urea 8-12, potassium dihydrogen phosphate 5-7, superphosphate 2-8, calcium magnesium phosphate fertilizer 2-8, potassium chloride 3-4, zinc sulfate 0.2-0.3, borax 0.3-0.4, fulvic acid iron solution 0.15-0.2, paclobutrazol 0.005-0.01, lincomycin fermentation liquid 10-100; Dissolve urea, potassium chloride, zinc sulfate and ferric humate solution in water and mix them evenly to prepare a nutrient solution; After 200 mesh zeolite is subjected to a high temperature treatment at 200°C, the above nutrient solution is sprayed onto the zeolite; Drying the zeolite in the sun or by drying it to reduce its moisture content to no more than 5%; The remaining chemical fertilizer is then evenly mixed with the modified accelerator to form lincomycin finished liquid fertilizer.
2. The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to claim 1, characterized in that: At 20-24 hours of cultivation, when amino nitrogen [NH2-N] ≤ 0.35 g / L, 1.067 g / L of ammonium sulfate was added.
3. The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to claim 2, characterized in that: During the 24th to 40th hour of cultivation, when the amino nitrogen was ≤0.55 g / L, 0.8 g / L of ammonium sulfate was added.
4. The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to claim 3, characterized in that: During the 40th to 140th hour of cultivation, when the amino nitrogen was ≤0.55 g / L, 0.533 g / L of ammonium sulfate was added.
5. The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to claim 4, characterized in that: At the 140th hour of cultivation, when the amino nitrogen is ≤0.55g / L, add 0.267g / L of ammonium sulfate.
6. The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to claim 5, characterized in that: The preparation process of the modified accelerator comprises the following steps: It is prepared from polylactic acid-glycolic acid copolymer and 6-benzylaminopurine in a mass ratio of 1:0.5-1; the sucrose ester biosurfactant is prepared from sucrose and esterifying agent palmitic acid under the catalysis of lipase.
7. The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to claim 6, characterized in that: Inoculate Paecilomyces cicadae on the slant culture medium and culture at a constant temperature of 25-30°C for 5-7 days for later use; Inoculate Paecilomyces cicadae into a 150 mL Erlenmeyer flask containing 50-55 mL of liquid culture medium, and culture on a shaker at 140-150 r / min and 25-30° C. for 72 h to 90 h; The seed-cultured Paecilomyces cicadae liquid was inoculated into a 500 mL Erlenmeyer flask containing 150 mL of liquid fermentation medium at an inoculation rate of 5-9%, and cultured on a shaking table at 150-160 r / min and 25-30° C. for 84 h to 96 h.
8. The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to claim 7, characterized in that: The components of the slant culture medium are 95-100 parts by weight of potato rhizome extract, 10-15 parts by weight of glucose, 15-20 parts by weight of agar, and 500 mL of distilled water, and the pH is adjusted to 6.0-7.
0.
9. The process for promoting the growth of cash crops by using a lincomycin finished liquid fertilizer according to claim 8, characterized in that: The components of the liquid culture medium are 10-13 parts by weight of glucose, 5-7 parts by weight of peptone, 4-6 parts by weight of yeast extract, 0.7-0.9 parts by weight of potassium dihydrogen phosphate, and 0.5-1 parts by weight of magnesium sulfate. Distilled water is added to 1000 mL, and the pH is adjusted to 6.5-7.
0.
10. The process for promoting the growth of cash crops using a lincomycin finished liquid fertilizer according to claim 9, characterized in that: The preparation steps of the liquid fermentation medium are as follows: 1500 mL of distilled water is added to the medium, and honey, citrus juice, glucose, peptone, yeast extract, soybean oil, potassium dihydrogen phosphate and magnesium sulfate are added in sequence, and the mixture is stirred continuously until completely dissolved, and the pH is adjusted to 6.8-7.2, and finally a modified growth promoter and a sucrose ester biosurfactant are added to obtain a liquid fermentation culture of Paecilomyces cicadae.
Citation Information
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