Composite device with ultrasonic cell disruption, denaturation and enzymolysis functions for biochemical detection

Abstract

The invention discloses a composite device with ultrasonic cell disruption, denaturation and enzymolysis functions for biochemical detection, the composite device comprises an ultrasonic cell disruption instrument, a pool body assembly and a pool body end cover, a mounting hole is formed in the middle of the pool body end cover, and the pool body end cover is provided with a plurality of liquid inlet connectors arranged around the mounting hole; the cell body assembly comprises a cell body with an inner cavity, the lower end face of the cell body end cover is installed at the position of an opening in the upper end of the cell body in a sealed mode, a probe of the ultrasonic cell lysis instrument penetrates through a cell body end cover installation hole and extends into the inner cavity of the cell body, and an outlet connector communicated with the inner cavity of the cell body is arranged on the lower portion of the cell body. The composite device meets the requirements of efficient cell disruption, heating protein denaturation, rapid heating and cooling and rapid enzymolysis, and is small in size and convenient to maintain.

Description

Technical Field

[0001] The invention relates to the technical field of biochemical detection, and in particular to a composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymolysis functions. Background Art

[0002] With the vigorous development of biotechnology, the research on the structure and function of biological macromolecules such as nucleic acids and proteins at the molecular level is increasing. Ultrasonic fragmentation has become an indispensable part of the sample preparation process, providing great convenience for experiments and research in many fields such as chemistry, biology, and medicine. In the process of protein research, there are many operations such as denaturation and enzymatic hydrolysis (heating-cooling-enzyme addition), which require a wide range of temperature changes. From ultrasonic fragmentation extraction to denaturation and enzymatic hydrolysis, this series of processes requires the conversion of the operating environment before sample processing, making the preparation process complicated and prone to contamination.

[0003] For example, Chinese patents CN202210937844.3 and CN202222733293.7 both mention the process of ultrasonic crushing and subsequent extraction and purification in the process of nucleic acid and protein purification and preparation. However, the entire system is relatively large in size, has a large sample processing capacity, and is highly specialized. A device that integrates ultrasonic crushing, denaturation, and enzymatic hydrolysis for a small number of laboratory samples (a few ml to more than ten ml) is more versatile in practical applications, while most ultrasonic crushing devices are equipped with sample temperature control devices, which can only extract samples and no longer perform the next step of sample preparation and processing, and are not suitable for biological laboratory research. Summary of the invention

[0004] In order to solve the technical problem that the sample processing from traditional ultrasonic disruption to denaturation and enzymatic hydrolysis requires conversion operation, which is complicated and easy to cause pollution, the present invention provides a composite device with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions for biochemical detection, which is a composite device with ultrasonic cell disruption and protein denaturation and enzymatic hydrolysis functions, and meets the requirements of efficient cell disruption, heated protein denaturation, fast temperature rise and fall, fast enzymatic hydrolysis, small size, easy maintenance, corrosion resistance, and less liquid sample residue.

[0005] The present invention adopts the following technical solution:

[0006] A composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions, the composite device comprising an ultrasonic cell lyser, a cell body assembly and a cell body end cover, a mounting hole formed in the middle of the cell body end cover, the cell body end cover is provided with a multi-way liquid inlet connector arranged around the mounting hole; the cell body assembly comprises a cell body with an inner cavity, the lower end face of the cell body end cover is sealed and mounted at an opening at the upper end of the cell body, the probe of the ultrasonic cell lyser passes through the cell body end cover mounting hole and extends into the inner cavity of the cell body, and the lower part of the cell body is provided with an outlet connector connected to its inner cavity.

[0007] Preferably, the cell body end cover is a cylindrical structure with a hollow chamber, the mounting hole is arranged in the middle of the upper end surface of the cell body end cover, the opening at the upper end of the cell body is connected to the through hole at the lower end surface of the cell body end cover, and the multi-way liquid inlet connector and the ultrasonic cell lysing instrument are respectively fixedly connected to the upper end surface of the cell body end cover.

[0008] Furthermore, a heater and a temperature controller are provided at the bottom of the inner cavity of the pool body, and the temperature controller controls the heating temperature of the heater according to the set temperature of the liquid in the pool body.

[0009] Preferably, the cell body is made of aluminum-magnesium alloy, its inner surface is coated with an anti-corrosion layer, and its outer surface forms a fin heat dissipation structure.

[0010] Furthermore, a heat dissipation device is installed on the outer surface of the pool body, which includes a cooling fin, a heat sink and an axial flow fan. The cooling fin, the heat sink and the axial flow fan are installed in sequence on the same side of the outer surface of the pool body.

[0011] Furthermore, the composite device also includes a bracket and a heat dissipation fan assembly, the lower end surface of the pool body and the heat dissipation fan assembly are respectively mounted on the bracket, and the heat dissipation fan assembly and the heat dissipation device are respectively arranged on both sides of the pool body.

[0012] Preferably, a heat insulating pad is provided between the cell body and the bracket.

[0013] Preferably, the outlet joint comprises a transverse joint and a vertical joint, the transverse joint is arranged at one side of the tank body, and the vertical joint is arranged at the lower end surface of the tank body.

[0014] Further preferably, the inner cavity of the cell body is an inverted cone structure.

[0015] The technical solution of the present invention has the following advantages:

[0016] A. The present invention combines an ultrasonic cell disruptor with a cell body assembly, so that the probe is extended into the inner cavity of the cell body, a multi-way liquid inlet connector is provided on the end cover of the cell body, and an outlet connector is provided at the lower part of the cell body. The probe contacts the prepared biological sample, and the cell can be quickly disrupted. Since the cell body is small in size, it can simultaneously meet the requirements of ultrasonic cell lysis, denaturation, and enzymatic hydrolysis for a small amount of laboratory samples (several ml to more than ten ml);

[0017] B. The present invention provides a heat dissipation device, a heater and a temperature controller on the composite device, which can realize the functions of rapid heating and rapid heat dissipation, and meet the requirements of normal working test of protein denaturation and enzymatic hydrolysis in biological detection;

[0018] C. The present invention sets the inner cavity of the cell body to an inverted cone structure design, and at the same time performs a coating treatment on the inner cavity surface of the cell body to achieve rapid sample drainage, reduce liquid residue and corrosion resistance requirements. It has a simple structure, is suitable for the preparation of biological liquid samples, and is easy to implement various complex functional tests. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the specific embodiments of the present invention, the drawings required for use in the specific embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 This is a schematic diagram of the overall structure of the composite device provided by the present invention;

[0021] Figure 2 for Figure 1 Schematic diagram of the combination of the end cover of the middle cell body and the probe of the ultrasonic cell lyser;

[0022] Figure 3 for Figure 1 Schematic diagram of the combined cell body assembly, cell body end caps and ultrasonic cell lyser probe.

[0023] The following are marked in the figure:

[0024] 1-Ultrasonic cell lyser

[0025] 11-Probe

[0026] 2-Pool body components

[0027] 21-Pool body

[0028] 22-Outlet connector

[0029] 221- horizontal joint, 222- vertical joint

[0030] 23- Heater

[0031] 24-Temperature Controller

[0032] 25-Heat dissipation device

[0033] 251- refrigeration fin, 252- heat sink, 253- axial flow fan

[0034] 3-Pool end cover

[0035] 31-Mounting hole

[0036] 4-Multi-way liquid inlet connector; 5-Bracket

[0037] 6-Cooling fan assembly

[0038] 61-support frame, 62-cooling fan

[0039] 7- thermal insulation pad; 8- sealing pad; 9- sealing ring. DETAILED DESCRIPTION

[0040] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0042] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0043] like Figure 1As shown, the present invention provides a composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymolysis functions, including an ultrasonic cell lysing instrument 1, a cell body assembly 2 and a cell body end cover 3, a mounting hole 31 is formed in the middle of the cell body end cover 3, and a multi-way liquid inlet connector 4 arranged around the mounting hole 31 is provided on the cell body end cover 3, and a five-way liquid inlet connector 4 is provided in the figure; the cell body assembly 2 includes a cell body 21 with an inner cavity, the lower end surface of the cell body end cover 3 is sealed and installed at the opening at the upper end of the cell body 21, the multi-way liquid inlet connector 4 is connected to the inner cavity of the cell body 21, the probe 11 of the ultrasonic cell lysing instrument 1 passes through the mounting hole 31 of the cell body end cover 3, and extends into the inner cavity of the cell body 21, and the lower part of the cell body 21 is provided with an outlet connector 22 connected with its inner cavity; liquid is injected into the inner cavity of the cell body from the multi-way liquid inlet connector 4. The ultrasonic cell disruptor 1 is threadedly connected to the cell body end cover 3, and sealed by a sealing ring 9; the cell body end cover 3 is threadedly connected to the cell body assembly 2, and a sealing gasket 8 is provided in between to achieve sealing between the two.

[0044] The ultrasonic cell lysis instrument probe used in the present invention is preferably made of titanium alloy TC4, which is immersed in the sample solution of the cell body 21. The immersion length of the probe 11 is designed for filling efficiency, and the material selection meets the corrosion resistance requirements, which can achieve efficient ultrasonic cell disruption requirements.

[0045] As a further preferred embodiment of the present invention, Figure 2 As shown, the cell body end cover 3 is a cylindrical structure with a hollow chamber, the mounting hole 31 is arranged in the middle of the upper end surface of the cell body end cover 3, the opening at the upper end of the cell body 21 is connected to the through hole at the lower end surface of the cell body end cover 3, and a sealing gasket 8 is arranged between the two for liquid sealing, and the multi-way liquid inlet connector 4 and the ultrasonic cell lysing instrument 1 are respectively fixed to the upper end surface of the cell body end cover 3 by threaded connection. The cell body end cover 3 is made of stainless steel 316L, and the multi-way liquid inlet connector 4 is at the top of the cell body end cover 3 and is fixed to the cell body end cover 3 by laser full welding. The center position of the cell body end cover 3 is designed for sealing and installing the ultrasonic cell disruptor 1, and the cell body end cover 3 is lined with a sealing gasket 8, which is sealed with the cell body assembly 2 by threaded connection to prevent liquid splashing when the ultrasonic cell disruptor 1 is working and causing leakage.

[0046] In order to better control the liquid temperature inside the cell body 21, as Figure 3 As shown, the present invention further provides a heater 23 and a temperature controller 24 at the bottom of the inner cavity of the pool body 21, and the temperature controller 24 controls the heating temperature of the heater 23 according to the set liquid temperature in the pool body 21. The heater 23 here can preferably be a heating rod, and the temperature controller 24 uses a pt100 platinum resistor, which can detect the liquid temperature and control the heating power of the heater 23 to achieve constant temperature control of the liquid.

[0047] Of course, in order to better dissipate the heat of the cell body 21, the cell body 21 in the present invention is preferably made of aluminum-magnesium alloy, and an anti-corrosion layer is coated on the inner surface of the inner cavity of the cell body 21, and the outer surface of the cell body 21 is designed as a fin heat dissipation structure, which significantly increases the heat dissipation area and meets the requirements of rapid heat dissipation. The volume of the cell body here is relatively small (15ml), and the volume of the cell body can be designed according to the required volume of the solution, and the inner wall of the cell body is coated to achieve anti-corrosion and reduce residual requirements.

[0048] In order to further meet the requirement of rapid cooling, the present invention also installs a heat dissipation device 25 on the outer surface of the pool body 21, which includes a cooling fin 251, a heat sink 252 and an axial flow fan 253. The cooling fin 251 is attached to the outer wall of the pool body 21, the heat sink 252 is installed on the outer side of the cooling fin 251, and the axial flow fan 253 is installed on the outer side of the heat sink 252.

[0049] In addition, the present invention installs the pool body assembly 1 on a bracket 5, and a heat insulation pad 4 is installed on the bottom end of the pool body 21 and the upper surface of the bracket 5 to play a heat insulation role; at the same time, a heat dissipation fan assembly 6 is arranged on the bracket 5, and the heat dissipation fan assembly 6 includes a support frame 61 and a heat dissipation fan 62, and the heat dissipation fan 62 is installed on the support frame 61, and the support frame 61 is installed on the bracket 5. The heat dissipation fan assembly 6 and the heat dissipation device 25 are respectively arranged on both sides of the pool body 21. The heat dissipation fan used here is a high-power fan, and the heat dissipation fan 62 is designed with an air duct guide and a gathering design, which realizes the rapid heat dissipation and cooling of the pool body 21 for the surface of the pool body 21 that needs heat dissipation.

[0050] As a further preferred embodiment of the present invention, the inner cavity of the pool body 21 adopts an inverted cone design to reduce liquid residue and the requirement for rapid liquid drainage.

[0051] like Figure 3 As shown in the structure, the outlet joint 22 provided at the lower part of the tank body 21 includes a transverse joint 221 and a vertical joint 222. The transverse joint 221 is provided at one side of the tank body 21, and the vertical joint 222 is provided at the lower end surface of the tank body 21. Since the bracket 5 supports the tank body 21, the material can also be discharged at the vertical joint 222 at the bottom. The outlet joint 22 here is preferably a standard threaded sealing joint. There are two liquid outlets at the bottom of the tank body 21, and its inner cavity is designed as an inverted cone, thereby reducing liquid residue and achieving the requirement of rapid liquid discharge.

[0052] The present invention adopts an ultrasonic cell disruptor 1 probe 11 and a denaturation enzymolysis cell body 21 to act on the same device, and quickly realizes ultrasonic cell disruption and denaturation, enzymolysis composite functions of liquid samples. The ultrasonic cell disruptor 1 is fixed on the end cover of the cell body 3, and the probe 11 extends into the cell body 21, with high disruption efficiency. Since the entire cell body 21 is small in volume, it meets the needs of ultrasonic disruption, denaturation and enzymolysis of a small amount of laboratory samples (several ml to more than ten ml). In addition, since the heating, heat dissipation and temperature control structures are designed on the same cell body, the rapid heating and cooling requirements of liquid samples in a short period of time are realized, and at the same time, the refrigeration sheet, heat sink and axial flow fan are fixed on the outer surface of the cell body in sequence, so as to realize rapid and efficient cooling of the sample. The present invention is simple in design and good in processability, and is very suitable for the preparation of biological liquid samples.

[0053] Anything not described in the present invention is applicable to the prior art.

[0054] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. However, the obvious changes or modifications derived therefrom are still within the scope of protection of the present invention.

Claims

1. A composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions, characterized in that: The composite device comprises an ultrasonic cell lysing instrument (1), a cell body assembly (2) and a cell body end cover (3); a mounting hole (31) is formed in the middle of the cell body end cover (3); a multi-way liquid inlet connector (4) arranged around the mounting hole (31) is provided on the cell body end cover (3); the cell body assembly (2) comprises a cell body (21) with an inner cavity; the lower end surface of the cell body end cover (3) is sealedly mounted on an opening at the upper end of the cell body (21); a probe (11) of the ultrasonic cell lysing instrument (1) passes through the mounting hole (31) of the cell body end cover (3) and extends into the inner cavity of the cell body (21); and an outlet connector (22) communicating with the inner cavity of the cell body (21) is provided at the lower part of the cell body (21).

2. The composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions according to claim 1, characterized in that: The cell body end cover (3) is a cylindrical structure with a hollow chamber, the mounting hole (31) is arranged in the middle of the upper end surface of the cell body end cover (3), the opening at the upper end of the cell body (21) is connected to the through hole at the lower end surface of the cell body end cover (3), and the multi-way liquid inlet connector (4) and the ultrasonic cell lysing instrument (1) are respectively fixedly connected to the upper end surface of the cell body end cover (3).

3. The composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions according to claim 1, characterized in that: A heater (23) and a temperature controller (24) are also provided at the bottom of the inner cavity of the pool body (21); the temperature controller (24) controls the heating temperature of the heater (23) according to the set temperature of the liquid in the pool body (21).

4. The composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions according to any one of claims 1 to 3, characterized in that: The pool body (21) is made of an aluminum-magnesium alloy, its inner cavity surface is coated with an anti-corrosion layer, and its outer surface forms a fin heat dissipation structure.

5. The composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions according to claim 4, characterized in that: A heat dissipation device (25) is also installed on the outer surface of the pool body (21), which includes a cooling fin (251), a heat sink (252) and an axial flow fan (253), and the cooling fin (251), the heat sink (252) and the axial flow fan (253) are installed in sequence on the same side of the outer surface of the pool body (21).

6. The composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions according to claim 5, characterized in that: The composite device also includes a bracket (5) and a heat dissipation fan assembly (6); the lower end surface of the pool body (21) and the heat dissipation fan assembly (6) are respectively mounted on the bracket (5); and the heat dissipation fan assembly (6) and the heat dissipation device (25) are respectively arranged on both sides of the pool body (21).

7. The composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions according to claim 6, characterized in that: A heat insulating pad (7) is provided between the cell body (21) and the bracket (5).

8. The composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions according to claim 1, characterized in that: The outlet joint (22) comprises a transverse joint (221) and a vertical joint (222); the transverse joint (221) is arranged at one side of the tank body (21), and the vertical joint (222) is arranged at the lower end surface of the tank body (21).

9. The composite device for biochemical detection with ultrasonic cell disruption, denaturation and enzymatic hydrolysis functions according to claim 1, characterized in that: The inner cavity of the pool body (21) is an inverted cone structure.

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