Method for detecting antibiotic residues in chicken

Through the methods of freezing pretreatment, specific solution system and multi-column serial solid-phase extraction, the problem of low extraction selectivity in the detection of antibiotic residues in chicken was solved, and efficient and accurate antibiotic detection was achieved.

CN119534730BActive Publication Date: 2025-10-10GUANGDONG INFINITY FOOD GRP CO LTD
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Patent Information

Application Number
CN202411601487.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-11-11
Publication Date
2025-10-10
Estimated Expiration
2044-11-11

AI Technical Summary

Technical Problem

The existing method for detecting antibiotic residues in chicken has the problem of low extraction selectivity, resulting in inaccurate detection results.

Method used

The method of freezing pretreatment, specific solution system extraction and multi-column series solid-phase extraction, including a combination of HLB, C18 and MCX solid-phase extraction columns, combined with ultrasound assistance and Raman spectrometer detection, improves the selectivity and efficiency of the extraction and purification process.

Benefits of technology

The extraction efficiency and purity of antibiotics in chicken were significantly improved, the detection accuracy was enhanced and it was suitable for rapid on-site detection. The repeatability and recovery rate of the test results were between 95-105%.

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Abstract

The application provides a method for detecting antibiotic residues in chicken, and belongs to the technical field of food detection. The method comprises the following steps: (1) freezing pretreatment of the chicken sample; (2) liquid phase extraction of the pretreated chicken sample, wherein the liquid phase extraction adopts a solution system, and the solution system comprises acidified acetonitrile, EDTA-2Na solution and phosphate; (3) enrichment and purification of the chicken extract, wherein the enrichment and purification adopts a series connection of an HLB solid phase extraction column, a C18 solid phase extraction column and an MCX solid phase extraction column; (4) drawing of standard curves of various antibiotics; and (5) detection of the sample to be measured by using a portable Raman spectrometer, and calculation of the content of each antibiotic in the sample according to the standard curves. The method uses freezing pretreatment, solution system extraction and the mode of enrichment and purification of three solid phase extraction columns, so that the extraction efficiency of the antibiotics in the chicken sample is significantly improved, and the detection accuracy of the antibiotics is improved.
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