Chrysomyia megacephala culture medium and chrysomyia megacephala polypeptide

Abstract

The invention relates to the technical field of chrysomyia megacephala extraction, in particular to a chrysomyia megacephala culture medium and a chrysomyia megacephala polypeptide extraction method, and the chrysomyia megacephala culture medium comprises wheat bran, brown sugar, meat meal, egg white, yeast powder, sodium chloride and water. According to the chrysomyia megacephala culture medium and the chrysomyia megacephala polypeptide extraction method, the culture medium is simple in component, low in cost, reasonable in nutrition collocation and suitable for chrysomyia megacephala growth, and chrysomyia megacephala cultured through the culture medium and treated through the method is full in polypide body, free of residues in the body, golden yellow in color and luster, free of fishy smell and free of toxic and side effects. The quality of a chrysomyia megacephala dry product is excellent, and the chrysomyia megacephala polypeptide extraction amount is remarkably increased through the culture medium and the method.

Description

Technical Field

[0001] The invention relates to the technical field of extracting Chrysopa longipes, and in particular to a Chrysopa longipes culture medium and a method for extracting Chrysopa longipes polypeptide. Background Art

[0002] The big-headed golden fly belongs to the order Diptera, family Calliphoridae, subfamily Chrysomelinae. It is a species widely distributed in China and is very common in the Yangtze River Basin. The larvae of the big-headed golden fly are rich in protein, fat and chitin, especially polypeptides are its main active ingredients. The dried larvae of the big-headed golden fly are the grain insects in traditional Chinese medicine. "Southern Yunnan Materia Medica" records that the grain insects have the functions of clearing away heat and detoxifying, eliminating food accumulation, and strengthening the spleen and stomach. They are mainly used to treat indigestion and infantile malnutrition.

[0003] The traditional method of raising big-headed golden flies leaves undigested food residues and excrement mixed in the insect bodies, and pathogenic bacteria remain on the surface of the insect bodies, which are still potential threats to their drug applications; the enzymes in the living larvae are still active, which will cause the insect bodies to oxidize and turn black, and some proteins will emit an unpleasant odor after degradation, affecting the quality of the medicinal materials; and the use of existing culture media to cultivate big-headed golden flies does not fully consider the nutritional ratio, resulting in low polypeptide content in the produced big-headed golden flies. When combined with traditional polypeptide extraction methods to extract polypeptides, there are problems such as low extraction rate of big-headed golden fly polypeptides and poor economic benefits.

[0004] Therefore, it is very necessary to propose a culture medium and an extraction method that can improve the quality of the dried Chrysopa spp. product and the extraction rate of the Chrysopa spp. polypeptide. Summary of the invention

[0005] The invention provides a Chrysopa melanogaster culture medium and a Chrysopa melanogaster polypeptide extraction method to solve the above problems.

[0006] In order to achieve the above purpose, the technical solution of the utility model is:

[0007] A culture medium for golden fly, which comprises wheat bran, brown sugar, meat powder, egg white, yeast powder, sodium chloride and water.

[0008] Furthermore, the content of each component is: wheat bran 500-700g, brown sugar 10-30g, meat powder 80-120g, egg white 10-30g, yeast powder 5-10g, sodium chloride 0.1-0.3g, water 1000-2000ml.

[0009] Furthermore, the content of each component is: 600g wheat bran, 20g brown sugar, 100g meat powder, 20g egg white, 8g yeast powder, 0.2g sodium chloride, and 1500ml water.

[0010] Meat meal is a high-protein substance made from one or more meats after being degreased, dehydrated and crushed. Egg white not only provides high-quality protein, but also contains a high amount of selenium, which can reach more than 2mg / kg. The enzymes contained in it can decompose the macromolecular proteins in the culture medium during the fermentation process, which is beneficial for the larvae to ingest. A small amount of brown sugar can provide the larvae with the necessary energy source.

[0011] A method for extracting Chrysopa longituba polypeptides comprises the following steps:

[0012] S1: taking the eggs of the big-headed golden fly and inoculating them into the big-headed golden fly culture medium described in claim 1, raising them until the big-headed golden fly begins to pupate, and collecting the living big-headed golden fly;

[0013] S2: drain all the internal residues of the big-headed golden fly, wash the live big-headed golden fly, place it in boiling water for 30-60 seconds, remove it and drain it, and dry it in an oven at 50-70°C until it is hard and brittle;

[0014] S3: Mix the dried Chrysopa longituba and physiological saline, squeeze the juice, heat in a water bath, centrifuge, and concentrate to obtain the Chrysopa longituba polypeptide extract.

[0015] Furthermore, in step S1, 500-1500 eggs of Chrysopa longituba are inoculated into every 100 g of culture medium.

[0016] Furthermore, in step S2, the method for draining the internal residue of the big-headed golden fly is: soak the big-headed golden fly in clean water for 3-5 hours, take it out and rinse it with 0.4% sodium hypochlorite solution and 75% ethanol in sequence, and then rinse it with distilled water. The living body of the big-headed golden fly is disinfected by soaking treatment combined with washing with sodium hypochlorite and ethanol, and then the residual reagent on the surface of the living body of the big-headed golden fly is washed away with distilled water, thereby reducing the influence of useless and harmful substances on the extracted polypeptide and avoiding affecting the efficacy of the drug.

[0017] Furthermore, in step S3, the volume ratio of Chrysopa longituba to physiological saline is 1:1-5.

[0018] Furthermore, in step S3, the water bath heating temperature is 50-70° C. and the heating time is 20-30 min.

[0019] The beneficial effects of the present invention are:

[0020] The present invention discloses a culture medium for Chrysopa longituba and a method for extracting Chrysopa longituba polypeptides. The culture medium has simple components, low cost, and reasonable nutritional combination, and is suitable for the growth of Chrysopa longituba. The dry product of Chrysopa longituba after being cultured with the culture medium and treated with the method has excellent quality, plump insect bodies, no residues in the bodies, golden color, and no fishy smell. At the same time, the culture medium and the method can significantly increase the amount of Chrysopa longituba polypeptide extracted. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] Preparation Example 1:

[0023] The components and contents of the Chrysopa longituba culture medium are as follows: 600 g of wheat bran, 20 g of brown sugar, 80 g of meat powder, 20 g of egg white, 8 g of yeast powder, 0.2 g of sodium chloride, and 1500 ml of water, used to prepare the Chrysopa longituba culture medium.

[0024] Preparation Example 2:

[0025] The components and contents of the Chrysopa longituba culture medium are as follows: 600 g of wheat bran, 20 g of brown sugar, 120 g of meat powder, 20 g of egg white, 8 g of yeast powder, 0.2 g of sodium chloride, and 1500 ml of water, used to prepare the Chrysopa longituba culture medium.

[0026] Preparation Example 3:

[0027] The components and contents of the Chrysopa longituba culture medium are as follows: 600 g of wheat bran, 20 g of brown sugar, 100 g of meat powder, 20 g of egg white, 8 g of yeast powder, 0.2 g of sodium chloride, and 1500 ml of water to prepare the Chrysopa longituba culture medium.

[0028] Preparation Example 4:

[0029] The components and contents of the Chrysopa longituba culture medium are as follows: 600 g of wheat bran, 20 g of brown sugar, 100 g of meat powder, 10 g of egg white, 8 g of yeast powder, 0.2 g of sodium chloride, and 1500 ml of water to prepare the Chrysopa longituba culture medium.

[0030] Preparation Example 5:

[0031] The components and contents of the Chrysopa longituba culture medium are as follows: 600 g of wheat bran, 20 g of brown sugar, 100 g of meat powder, 30 g of egg white, 8 g of yeast powder, 0.2 g of sodium chloride, and 1500 ml of water to prepare the Chrysopa longituba culture medium.

[0032] The best preparation example is Preparation Example 3.

[0033] Embodiment 1:

[0034] The components and contents of the Chrysopa spp. culture medium are as follows: 600 g of wheat bran, 20 g of brown sugar, 100 g of meat powder, 20 g of egg white, 8 g of yeast powder, 0.2 g of sodium chloride, and 1500 ml of water, to prepare the Chrysopa spp. culture medium;

[0035] Extraction of Chrysopa longituba peptides:

[0036] S1: Take the eggs of the big-headed golden fly and inoculate them into the big-headed golden fly culture medium described in claim 1, inoculate 1000 eggs in every 100g of the culture medium, use drinking water, control the culture humidity to 70%, pH to 6.5, and temperature to 30°C, stir once in the morning and evening every day under natural light, raise the big-headed golden fly until it grows to the 3rd instar larvae and begins to turn yellow, and collect the big-headed golden fly larvae;

[0037] S2: Soak the big-headed gold fly in clean water for 4 hours, drain the internal residue of the big-headed gold fly, take out the big-headed gold fly, rinse it with 0.4% sodium hypochlorite solution and 75% ethanol in sequence, and finally rinse it with an equal volume of distilled water three times, put the big-headed gold fly in boiling water for 60 seconds, take it out and drain it, and dry it in a 60°C oven until it is hard and brittle, and the surface is yellow, to obtain the dried big-headed gold fly (dried big-headed gold fly);

[0038] S3: Mix the dried Chrysopa longituba with 0.9% saline in a volume ratio of 1:3, squeeze the juice, heat in a 60°C water bath for 25 minutes, centrifuge at 8000r / min for 15 minutes, collect the supernatant containing the polypeptide component, and concentrate it to obtain the Chrysopa longituba polypeptide extract.

[0039] Embodiment 2:

[0040] The only difference between this embodiment and embodiment 1 is that, in this embodiment, the components and contents of the Chrysopa longituba culture medium are as follows: 700g of wheat bran, 30g of brown sugar, 100g of meat powder, 20g of egg white, 8g of yeast powder, 0.2g of sodium chloride, and 1500ml of water, to prepare the Chrysopa longituba culture medium.

[0041] Embodiment 3:

[0042] The only difference between this embodiment and embodiment 1 is that, in this embodiment, 1500 insect eggs are inoculated into every 100 g of culture medium.

[0043] Embodiment 4:

[0044] The only difference between this embodiment and embodiment 1 is that, in this embodiment, in step S3 of extracting Chrysopa longituba polypeptide, the water bath heating time is 30 minutes.

[0045] Embodiment 5:

[0046] The only difference between this embodiment and embodiment 1 is that, in this embodiment, in step S3 of extracting Chrysopa longituba polypeptide, the water bath heating time is 20 minutes.

[0047] Embodiment 6:

[0048] The only difference between this embodiment and embodiment 4 is that in this embodiment, in step S3 of extracting Chrysopa longipes polypeptides: dried Chrysopa longipes is mixed with physiological saline having a mass concentration of 0.9% in a volume ratio of 1:1.

[0049] Embodiment 7:

[0050] The only difference between this embodiment and embodiment 1 is that in this embodiment, in step S3 of extracting Chrysopa longipes polypeptides: dried Chrysopa longipes is mixed with physiological saline having a mass concentration of 0.9% at a volume ratio of 1:5.

[0051] Embodiment 8:

[0052] The only difference between this embodiment and embodiment 1 is that, in this embodiment, in step S2 of extracting Chrysopa longituba polypeptide, the time of soaking Chrysopa longituba in clean water is 3 hours.

[0053] Embodiment 9:

[0054] The only difference between this embodiment and embodiment 1 is that, in this embodiment, in step S2 of extracting Chrysopa longituba polypeptide, the time of soaking Chrysopa longituba in clean water is 5 hours.

[0055] Comparative Example 1:

[0056] The only difference from Example 1 is that in this comparative example, no meat powder and egg white are added to the culture medium of Chrysocytis melanogaster, and other ingredients remain unchanged.

[0057] Comparative Example 2:

[0058] The only difference from Example 1 is that in this comparative example, in step S2 of extracting Chrysopa longituba polypeptide, Chrysopa longituba is not immersed in clean water.

[0059] Comparative Example 3:

[0060] The only difference from Example 1 is that in this comparative example, in step S2 of extracting the Chrysopa longituba polypeptide, the Chrysopa longituba insect body is not placed in boiling water for treatment.

[0061] Test Example 1:

[0062] The quality of the large-headed golden flies cultured in Examples 1-9 and Comparative Examples 1-3 (whether the insect body is full, whether there is residue in the insect body, whether there is a fishy smell) was observed and tested, and the extracted polypeptides were used as samples to determine the polypeptide content using a BCA protein concentration detection kit (Takara, catalog number T9300A). The results are shown in the following table:

[0063]

[0064] It can be seen from the table that the dried Chrysopa longituba cultured with the Chrysopa longituba culture medium provided by the present invention and treated with the present method have full insect bodies, no residue in the body, golden color and no fishy smell, and excellent quality. In addition, the extraction amount of the Chrysopa longituba cultured with the present culture medium and the polypeptide extracted with the present method is relatively high.

[0065] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A culture medium for Chrysopa longituba, characterized in that: The culture medium consists of wheat bran, brown sugar, meat powder, egg white, yeast powder, sodium chloride and water.

2. A culture medium for Chrysopa longituba according to claim 1, characterized in that: The content of each component is: wheat bran 500-700g, brown sugar 10-30g, meat powder 80-120g, egg white 10-30g, yeast powder 5-10g, sodium chloride 0.1-0.3g, water 1000-2000ml.

3. A culture medium for Chrysopa longituba according to claim 2, characterized in that: The content of each component is: 600g wheat bran, 20g brown sugar, 100g meat powder, 20g egg white, 8g yeast powder, 0.2g sodium chloride, and 1500ml water.

4. A method for extracting Chrysopa longituba polypeptides, comprising the following steps: S1: taking the eggs of the big-headed golden fly and inoculating them into the big-headed golden fly culture medium described in claim 1, raising them until the big-headed golden fly begins to pupate, and collecting the living big-headed golden fly; S2: draining the internal residue of the big-headed golden fly body obtained in step S1, washing the big-headed golden fly body, placing it in boiling water for 30-60 seconds, taking it out and draining it, and drying it in an oven at 50-70° C. until it is hard and brittle; S3: Mix the dried Chrysopa longituba and physiological saline, squeeze the juice, heat in a water bath, centrifuge, and concentrate to obtain the Chrysopa longituba polypeptide extract.

5. The method for extracting Chrysopa longituba polypeptide according to claim 4, characterized in that: In step S1, 500-1500 eggs of Chrysopa longituba are inoculated into every 100 g of culture medium.

6. The method for extracting Chrysopa longituba polypeptide according to claim 4, characterized in that: In step S2, the method for draining the internal residues of the big-headed golden fly body is as follows: soak the big-headed golden fly in clean water for 3-5 hours, take it out and rinse it with 0.4% sodium hypochlorite solution and 75% ethanol in sequence, and then rinse it with distilled water.

7. The method for extracting Chrysopa longituba polypeptide according to claim 4, characterized in that: In step S3, the volume ratio of Chrysopa longituba to physiological saline is 1:1-5.

8. The method for extracting Chrysopa longituba polypeptide according to claim 4, characterized in that: In step S3, the water bath heating temperature is 50-70° C. and the heating time is 20-30 min.

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