Pesticide composition compounded by ametoctradin and zoxamide

Abstract

The invention provides an ametoctradin and zoxamide compounded pesticide composition. The ametoctradin and zoxamide compounded pesticide composition comprises ametoctradin, zoxamide and auxiliary materials, the mass ratio of ametoctradin to zoxamide is (1 to 50): (1 to 50); the total amount of ametoctradin and zoxamide accounts for 1%-90% of the mass of the pesticide composition. Dosage forms of the pesticide composition comprise a microcapsule suspending agent, a suspending agent, wettable powder, an oil-based suspoemulsion, an emulsion in water, a microemulsion, a water dispersible granule and a granule. The pesticide composition is used for preventing and treating epidemic diseases caused by fungal infection of crops, especially has better antibacterial and bactericidal effects on tobacco black shank, and has the advantages of improving the bactericidal effect, reducing the dosage and controlling the drug resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular relates to a fungicide composition for resisting tobacco black shank, and more particularly to a pesticide composition of pyraclostrobin and zoxystrobin. Background Art

[0002] Tobacco black shank is an important soil-borne fungal disease on tobacco caused by infection with tobacco phytophthora. Tobacco phytophthora belongs to the subphylum Mastozotocin, class Oomycetes, order Peronosporales, and genus Phytophthora. For this disease, the commonly used drugs according to different seedling stages include: (1) Before transplanting, spray the seedbed evenly with 500 times diluted 58% metalaxyl manganese zinc wettable powder. (2) When transplanting, apply 350-400 g / mu of 55% dichlorodiphenyltrichloroethane wettable powder in holes. (3) Before the tobacco plants in the field become diseased, use 600-800 times diluted 58% metalaxyl manganese zinc wettable powder, 600 times diluted 40% metalaxyl copper wettable powder, 400-600 times diluted 64% metalaxyl manganese zinc wettable powder, 400 times diluted 90% fosetyl aluminum wettable powder, or 70% metalaxyl. Thiram WP 600-800 times diluted, or 55% sodium disulfone WP 500 times diluted, spray once every 15 days. After the tobacco plants are filled with soil and before the disease occurs, use the root irrigation method of local protection to apply 33.5% copper quinoline suspension 1500-2000 times diluted to the stems and the soil surface, which has a significant effect.

[0003] Azopyram is a highly selective fungicide that can effectively and flexibly control downy mildew and late blight. The product has the advantages of being resistant to rain erosion, being able to redistribute in leaves, protecting the healthy growth of crops, and giving full play to growth potential. Azopyram is a QoSI fungicide, and its mechanism of action is that it binds to the Qo-distal site (Qo-distal) on the bc1 complex (complex III) on the mitochondria, blocking the electron transfer of the respiratory electron transport chain. The problem of drug resistance of this type of fungicide is also very prominent, which can easily cause rapid mutation of bacterial strains. There is no single preparation of azopyram on the market, because the drug is very likely to cause drug resistance when used alone.

[0004] Zoxystrobin is a highly effective protective fungicide of the benzamide class. It inhibits sclerotial division by binding to tubulin β-subunits and disrupting the microtubule cytoskeleton. It does not affect the motility, cyst formation or germination of zoospores. Along with the first cycle of sclerotial division, the elongation of the germ tube is inhibited, thereby preventing the pathogen from penetrating the host plant. Zoxystrobin can prevent and control diseases caused by Oomycetes on crops. Currently, zoxystrobin and its compound preparations on the market are mainly used for cucumber downy mildew, potato late blight, tomato late blight and grape downy mildew, but the content of active ingredients in the preparations usually needs to reach more than 75%, the dosage is large and the cost is high, and it is more likely to cause drug resistance.

[0005] Based on the above situation, we can see that: (1) The active ingredient content of the commonly used drugs for tobacco black shank must be configured to a higher concentration. Secondly, the commonly used drugs are still mainly composed of conventional ingredients such as metalaxyl and mancozeb. The long-term use of conventional drugs at high concentrations has led to the emergence of a large number of drug-resistant strains. (2) Both pyraclostrobin and zoxanil are relatively new and highly effective fungicides, but the use of either alone will also cause serious drug resistance problems.

[0006] In response to the above problems, our company has conducted research and combined two highly effective fungicides, pyraclostrobin and zoxamidoprop, with different mechanisms of action, to prevent tobacco black shank disease, in order to improve the fungicide effect and control the development of drug resistance. Summary of the invention

[0007] The object of the present invention is to provide a pesticide with better antibacterial and bactericidal efficacy against tobacco black shank disease, so as to improve the bactericidal effect, reduce the dosage of the pesticide, and control the problem of drug resistance.

[0008] To achieve the above object, the present invention adopts the following technical solution:

[0009] A pesticide composition of pyraclostrobin and zoxamide, comprising pyraclostrobin, zoxamide and auxiliary materials; the mass ratio of pyraclostrobin to zoxamide is (1-50):(1-50); the total amount of pyraclostrobin and zoxamide accounts for 1% to 90% of the mass percentage of the pesticide composition.

[0010] Preferably, the ratio of the pyraclostrobin to the zoxadiazole is (1-50):(1-40).

[0011] More preferably, the ratio of the pyraclostrobin to the zoxadiazole is (1-40):(10-30).

[0012] More preferably, the ratio of the pyraclostrobin to the zoxadiazole is (1-30):(1-20).

[0013] Preferably, the total amount of the pyraclostrobin and the zoxadiazole accounts for 10% to 65% by mass of the pesticide composition.

[0014] More preferably, the total amount of the pyraclostrobin and the zoxadiazole accounts for 20% to 50% by mass of the pesticide composition.

[0015] According to laboratory tests, the ratio of the pyraclostrobin and the oxazolidinone can be: 15:1, 10:1, 8:1, 6:1, 4:1, 2:1, 3:2, 5:4, 1:1, 4:5, 2:3, 1:2, 1:4, 1:6, 1:8, 1:10, 1:15, and the like.

[0016] According to laboratory tests, the mass percentage of the total amount of the pyraclostrobin and the oxazolidinone in the pesticide composition can be: 1%, 5%, 10%, 15%, 18%, 20%, 24%, 27%, 30%, 33%, 36%, 40%, 42%, 45%, 48%, 50%, 55%, 60%, 65%, 70%, 80%, 90%, and the like.

[0017] Preferably, according to laboratory test results, the ratio of the pyraclostrobin to the zoxamide is 2:1 to 1:2; most preferably, the ratio of the pyraclostrobin to the zoxamide is 5:4, and the total amount of the pyraclostrobin and the zoxamide accounts for 27% to 45% by mass of the pesticide composition.

[0018] Furthermore, the pesticide composition of the compound of pyraclostrobin and zoxadiazole can be prepared into dosage forms including microcapsule suspensions, suspensions, wettable powders, oil-based suspoemulsions, emulsions in water, microemulsions, water-dispersible granules, granules, and the like.

[0019] According to an embodiment of the present invention, the pesticide composition comprises the following components and contents when prepared into a microcapsule suspension: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 10% of a polymer capsule wall material, 2% to 10% of a dispersant, 1% to 10% of an organic solvent, 1% to 10% of an emulsifier, 0.1% to 5% of a pH regulator, 0.01% to 2% of a defoamer, 0.1% to 8% of an antifreeze agent, and the remainder is deionized water.

[0020] According to an embodiment of the present invention, the pesticide composition comprises the following components and contents when prepared as a suspension: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 12% of a dispersant, 1% to 10% of a wetting agent, 0.1% to 12% of a thickener, 0.1% to 8% of an antifreeze agent, and the balance is deionized water.

[0021] According to an embodiment of the present invention, the pesticide composition comprises the following components and contents when prepared as a wettable powder: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 10% of a dispersant, 1% to 8% of a wetting agent, 0.1% to 5% of a stabilizer, and the remainder is filler.

[0022] According to an embodiment of the present invention, the pesticide composition comprises the following components and contents when prepared as an oil-based suspoemulsion: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 50% of oil, 1% to 8% of emulsifier, 1% to 15% of dispersant, 1% to 8% of antifreeze agent, 0.1% to 5% of stabilizer, and the balance is deionized water.

[0023] According to an embodiment of the present invention, the pesticide composition comprises the following components and contents when prepared as an aqueous emulsion: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 60% of an organic solvent, 1% to 12% of an emulsifier, 0.1% to 8% of an antifreeze agent, 0.01% to 2% of a defoamer, 0.1% to 2% of a thickener, and the balance is deionized water.

[0024] According to an embodiment of the present invention, the pesticide composition comprises the following components and contents when prepared into a microemulsion: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 25% of a solvent, 10% to 35% of an emulsifier, 5% to 15% of a cosolvent, 3% to 5% of an antifreeze agent, and the remainder is deionized water.

[0025] According to an embodiment of the present invention, the pesticide composition comprises the following components and contents when prepared into water-dispersible granules: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 12% of dispersant, 1% to 8% of wetting agent, 1% to 10% of disintegrant, and the balance is filler.

[0026] According to an embodiment of the present invention, the pesticide composition comprises the following components and contents when made into granules: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 12% of dispersant, 1% to 10% of wetting agent, 0.1% to 8% of binder, and the balance is filler.

[0027] According to an embodiment of the present invention, when the pesticide composition is prepared into the above-mentioned formulation, it may further include 0.1% to 2% of a preservative.

[0028] Preferably, the wetting agent is selected from naphthalene sulfonate, sodium dodecyl sulfate, sodium secondary alkyl sulfate, alkyl sulfonate, trisiloxane polyoxyethylene ether, sodium N-lauroyl glutamate, sodium lauroyl sarcosinate, sodium methylnaphthalene sulfonate formaldehyde condensate, castor oil polyoxyethylene ether, triphenyl ethyl phenol polyoxyethylene ether, sodium dodecylbenzene sulfonate, sodium alkylnaphthalene sulfonate, sodium isooctyl succinate sulfonate, fatty alcohol polyether glycerol fatty acid polyoxyethylene ether, oxyethylene-polyoxypropylene block copolymer, polyethylene glycol ether of branched or linear alcohol, AEO series, polyvinyl alcohol, polyoxyalkylene amine derivatives, polyvinyl pyrrolidone, copolymer of polyvinyl alcohol and polyvinyl pyrrolidone, copolymer of (meth) acrylic acid and (meth) acrylate, opening powder BX, etc., one or more thereof.

[0029] Preferably, the dispersant is selected from one or more of lignin sulfonate, polycarboxylate, sodium tripolyphosphate, alkyl naphthalene sulfonate formaldehyde condensate, alkyl succinate, glycerol fatty acid polyoxyethylene ether, polyoxyethylene alkyl aryl ether, naphthalene sulfonate formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, naphthalenesulfonic acid formaldehyde condensate sodium salt, nonylphenol polyoxyethylene ether, polyoxyethylene lanolin alcohol, alkylphenol polyoxyethylene ether formaldehyde condensate, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, polyoxyethylene sorbitan fatty acid ester, phosphate, and the like.

[0030] Preferably, the emulsifier is selected from one or more of alkylphenol polyoxyethylene ether sulfosuccinate, fatty acid polyoxyethylene ether, polyoxyethylene fatty alcohol ether, ethoxylated castor oil, polymethyl acrylate, phenylphenol polyoxyethylene ether phosphate, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt, benzyl dimethylphenol polyoxyethyl ether, sorbitan fatty acid ester polyoxyethylene ether, sorbitan oleate (Span-80), nonylphenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether phosphate, guar gum, glycerol, and the like.

[0031] Preferably, the defoaming agent is selected from organic silicone, C 8-10 Fatty alcohol, C 10-20 One or more of saturated fatty acid, silicone oil, n-octanol, butyl phosphate, isobutyl phosphate, etc.

[0032] Preferably, the disintegrant is selected from one or more of ammonium sulfate, calcium chloride, sodium chloride, magnesium chloride, aluminum chloride, sodium sulfate, sodium bicarbonate, calcium sulfate, and the like.

[0033] Preferably, the stabilizer is selected from one or more of triethanolamine, epichlorohydrin, butyl glycidyl ether, triphenyl phosphite, N-soybean oil-based trimethylene diamine, dialkyl succinic acid acetate sulfonate, and the like.

[0034] Preferably, the binder or thickener is selected from one or more of glucose, sucrose, hydroxyethyl cellulose, soluble starch, polyethylene glycol, methyl cellulose, paraffin, gypsum, hydroxypropyl methyl cellulose, polyacrylamide, xanthan gum, urea-formaldehyde resin, gelatin, gum arabic, carboxymethyl cellulose, carboxyethyl cellulose, magnesium aluminum silicate, polyvinyl acetate, sodium alginate, and the like.

[0035] Preferably, the preservative is selected from one or more of potassium sorbate, formaldehyde, benzoic acid, sodium benzoate, kason, isothiazolinone, and the like.

[0036] Preferably, the antifreeze agent is selected from one or more of ethylene glycol, propylene glycol, glycerol, sorbitol, urea, inorganic salts, and the like.

[0037] Preferably, the oil agent is selected from one or more of vegetable oil, mineral oil, paraffin oil, etc.

[0038] Preferably, the organic solvent is selected from one or more of methyl oleate, ethyl acetate, fatty acid methyl ester, isooctyl alcohol, sec-butyl acetate, dimethyl carbonate, N-methylpyrrolidone, N,N-dimethylacetamide, methanol, DMSO, acetone, isopropanol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, and the like.

[0039] Preferably, the filler includes a solid filler and a liquid filler. The solid filler is selected from one or more of corn starch, white carbon black, kaolin, bentonite, light calcium carbonate, gypsum, attapulgite, diatomaceous earth, clay, sepiolite, montmorillonite, calcium carbonate, talc, etc. The liquid filler is selected from one or more of soybean oil, castor oil, mineral oil, etc.

[0040] Preferably, the polymer capsule wall material is selected from one or more of polyacrylic acetate, cellulose phthalate, polyacrylate, polyamide, multifunctional amino plastics, hydroxyethyl cellulose, sodium polystyrene sulfonate, urea-formaldehyde resin, and the like.

[0041] Preferably, the pH adjuster is selected from one or more of hydrochloric acid, acetic acid, sodium hydroxide, ammonium sulfate, citric acid-sodium citrate buffer, and the like.

[0042] Preferably, the co-solvent is selected from one or more of sodium benzoate, urea, niacinamide, etc.

[0043] Preferably, the preferred dosage form of the pesticide composition is a suspension concentrate, a wettable powder, or an oil-based suspoemulsion.

[0044] The present invention also provides the use of the pesticide composition of pyraclostrobin and zoxystrobin, and the use of the pesticide composition is to prevent and treat diseases of crops caused by fungal infection. The diseases caused by fungal infection include tobacco black shank, cucumber downy mildew, potato late blight, tomato late blight, grape downy mildew, etc. In particular, the pesticide composition is particularly suitable for preventing and treating tobacco black shank.

[0045] The advantages of the present invention are:

[0046] (1) The pesticide composition of pyraclostrobin and zoxadiazole of the present invention has a high control effect on diseases caused by fungal infection of crops.

[0047] (2) The fungicidal mechanisms of pyraclostrobin and benomyl are different. Compared with their single agents, the pesticide composition formed by the combination of the two has a significant synergistic effect on the prevention and treatment of fungal diseases. It can reduce the amount of active ingredients used, reduce costs and reduce pesticide residues. Moreover, the compound pesticide composition can effectively overcome the problem of drug resistance and extend the service life of the agent. DETAILED DESCRIPTION

[0048] In order to make those skilled in the art better understand the technical solution of the present invention, some non-limiting embodiments are disclosed below to further illustrate the present invention in detail. It should be noted that the embodiments disclosed below are only preferred solutions, some test examples, some embodiments, and some comparative examples of the invention, and are not the entire contents of the present invention. Various reagents in the following embodiments and test examples are all purchased from the market.

[0049] 1. Effect of active ingredient ratio on antibacterial effect

[0050] 1. Suspension Example

[0051] Example 1 to Example 9 (EX-1 to EX-9): A suspension was used as a dosage form to prepare preparations having a total active ingredient concentration of 36% and a ratio of Ametoctradin to Zoxamide of 4:1, 2:1, 3:2, 5:4, 1:1, 4:5, 2:3, 1:2, and 1:4, respectively.

[0052] Comparative Example: Comparative Example 1 (CTR-1) and Comparative Example 2 (CTR-2) were prepared using the same auxiliary materials as in the above examples. The active ingredient in Comparative Example 1 was 30% of pyraclostrobin, and the active ingredient in Comparative Example 2 was 30% of oxadiazine.

[0053] The efficacy of these preparations against tobacco Phytophthora strains was tested in the laboratory using the Oxford cup inhibition zone method.

[0054] Table 1 Formulas of various agents in antibacterial experiments

[0055]

[0056] Note: A / Z: is the ratio of mefenamic acid to zoxamidin.

[0057] The preparation method of the above formula is as follows: (1) first disperse all auxiliary materials except the thickener (carboxymethyl cellulose and magnesium aluminum silicate) into 1 / 2 amount of deionized water, and homogenize and disperse them; (2) add the active ingredients (pyraclostrobin and oxadiazine) to the mixed solution of step (1), and disperse them evenly by high-speed homogenization at 13000rpm~18000rpm; then place it in a sand mill for coarse grinding; (3) continue to add the thickener to the sand mill, and also add the remaining 1 / 2 amount of deionized water to the sand mill; then use finer zirconium beads to finely grind until D98≤5.0μm to obtain the suspension.

[0058] 2. Antibacterial experiment:

[0059] The antibacterial effects of Comparative Example 1, Comparative Example 2, and Examples 1 to 9 on Phytophthora nicotianae were tested by the Oxford cup inhibition zone method.

[0060] Test pathogen: tobacco phytophthora strains were collected from the rhizomes of diseased plants in tobacco fields in Lichuan City, Hubei Province, and then isolated and cultured. The mixture was diluted to 1×10 8 cfu / mL of bacterial suspension.

[0061] Determination of inhibition zone: (1) The diameter of the inhibition zone is greater than 20 mm, which is extremely sensitive (++++); (2) The diameter of the inhibition zone is between 20 and 15 mm, which is highly sensitive (+++); (3) The diameter of the inhibition zone is between 15 and 10 mm, which is highly sensitive (++); (4) The diameter of the inhibition zone is less than 10 mm, which is slightly sensitive (+); (5) No inhibition zone is insensitive (-). The antibacterial effects of the active ingredients of each agent are shown in Table 2.

[0062] Table 2 Antibacterial test results

[0063] Pharmacy Diameter of inhibition zone (mm) Antibacterial level Comparative Example 1 11.63 ++ Comparative Example 2 10.32 ++ Example 1 13.04 ++ Example 2 14.27 ++ Example 3 16.68 +++ Example 4 21.87 ++++ Example 5 18.96 +++ Example 6 18.63 +++ Example 7 14.39 ++ Example 8 13.71 ++ Example 9 12.83 ++

[0064] From Table 2 we can see that:

[0065] (1) The antibacterial effect of the combined preparation of pyraclostrobin and zoxanil is better than that of the single preparations of either.

[0066] (2) Among Examples 1 to 9, the antibacterial levels of Example 1 and Example 9 are slightly lower, indicating that the greater the difference in the compounding ratio of pyraclostrobin and bezoclostrobin, the worse the antibacterial effect; when the compounding ratio of pyraclostrobin and bezoclostrobin is between 2:1 and 1:2, the antibacterial effects are close and better, among which the antibacterial effect is best when the compounding ratio of pyraclostrobin and bezoclostrobin is 5:4, and on the other hand, it also indicates that pyraclostrobin and bezoclostrobin will form a synergistic effect after compounding.

[0067] 2. Examples of different dosage forms (Examples 10 to 16)

[0068] 1. Example 10: 18% pyraclostrobin·zoxanil microcapsule suspension

[0069] Preparation components: 10% of pyraclostrobin, 8% of zoxamide, 10% of cellulose phthalate, 2% of nonylphenol polyoxyethylene ether, 5% of polyoxyethylene sorbitan fatty acid ester, 10% of N-methylpyrrolidone, 7% of phenylphenol polyoxyethyl ether phosphate, 2.5% of acetic acid, 2% of isobutyl phosphate, 1% of glycerol, and the balance is deionized water.

[0070] Preparation method: Mix the above materials in proportion, adjust the pH value to 4-5, and polymerize under acid catalysis to wrap the oil phase material to form microcapsule particles, slowly heat up and solidify, the temperature is controlled at 40-50℃, and the solidification time is 1h. Add appropriate amount of auxiliary agent to obtain the product.

[0071] 2. Example 11: 45% pyraclostrobin·zoxanil wettable powder

[0072] Preparation components: 25% of azoxystrobin, 20% of zoxamide, 5% of sodium salt of naphthalenesulfonic acid formaldehyde condensate, 3% of sodium lignin sulfonate, 3% of sodium isooctyl succinate sulfonate, 3% of BX powder, 3% of sodium dialkyl succinate acetonide sulfonate, and the balance is bentonite.

[0073] Preparation method: Place the above raw materials in a constant temperature drying oven and dry them at 100°C. After baking for 6 to 8 hours, cool them to below 30°C and take them out. Crush them to a particle size of about 0.12 mm. Weigh the active ingredients and fillers in proportion, mix them thoroughly and crush them to pass through a 325 mesh sieve. Add wetting agents, dispersing aids and other auxiliary materials, mix them evenly, and ensure that the powder has good wettability and dispersibility.

[0074] 3. Example 12: 27% pyraclostrobin·zoxanil oil-based suspoemulsion

[0075] Preparation components: 15% of pyraclostrobin, 12% of zoxamide, 35% of mineral oil, 6% of alkylphenol polyoxyethylene ether sulfosuccinate, 8% of octylphenol polyoxyethylene ether, 2% of propylene glycol, 1.5% of triethanolamine, and the balance is deionized water.

[0076] Preparation method: Grind the active ingredient to D at room temperature 90 <5μm and mix evenly; mix the active ingredient, oil agent and dispersant evenly; mix the water phase material and stabilizer evenly; heat the oil phase and water phase to about 60℃~70℃, stir them respectively until they are evenly mixed, then slowly add the water phase into the oil phase and keep stirring until they are completely mixed.

[0077] 4. Example 13: 54% pyraclostrobin·zoxanil water dispersible granules

[0078] Preparation components: 30% of pyraclostrobin, 24% of oxadiazine, 9% of sodium polyoxyethylene fatty alcohol ether sulfate, 5% of polyvinyl pyrrolidone, 6.5% of calcium chloride, and the balance is a mixed powder of corn starch and calcium carbonate.

[0079] Preparation method: All active ingredients and auxiliary materials are mixed uniformly according to the above formula, crushed with an ultra-fine airflow mill, kneaded, and then added into a fluidized bed granulator for granulation, drying, sieving, and sampling and analysis to obtain the product.

[0080] 5. Example 14: 45% pyraclostrobin·zoxanil granules

[0081] Preparation components: 25% of pyraclostrobin, 20% of zoxamide, 10% of phosphate ester, 8% of sodium N-lauroyl glutamate, 8% of carboxyethyl cellulose, and the balance is a mixed powder of montmorillonite, calcium carbonate and talc.

[0082] Preparation method: The above active ingredients and excipients are crushed separately and sieved through a 200-mesh sieve, the treated active ingredients and excipients except the binder are mixed according to the formula, and then an appropriate amount of binder is added and granulated by a granulator; the prepared wet granules are dried and sieved through a 150-mesh sieve.

[0083] 6. Example 15: 36% pyraclostrobin·zoxanil emulsion in water

[0084] Preparation ingredients: pyraclostrobin 25%, oxadiazine 20%, isopropanol 50%, fatty alcohol polyoxyethylene ether 12%, urea 6%, C 8-10 0.8% fatty alcohol, 1% xanthan gum, 1% hydroxypropyl methylcellulose, 0.5% potassium sorbate, and the balance is deionized water.

[0085] Preparation method: Grind the active ingredient to D at room temperature 90 <5μm and mix evenly; mix the active ingredients and organic solvent evenly as the oil phase; mix water and thickener evenly as the water phase; heat the oil phase and water phase to about 70℃, stir them separately until they are even, then slowly add the water phase to the oil phase and stir continuously until they are completely mixed. After cooling to room temperature, add preservatives, defoaming agents and other auxiliary ingredients.

[0086] 7. Example 16: 27% pyraclostrobin·zoxanil microemulsion

[0087] Preparation components: 15% of pyraclostrobin, 12% of oxadiazine, 5% of fatty acid methyl ester, 20% of isopropyl alcohol, 10% of benzyl dimethylphenol polyoxyethyl ether, 6% of castor oil polyoxyethylene ether phosphate, 7% of urea, 3% of ethylene glycol, and the balance is deionized water.

[0088] Preparation method: Grind the active ingredient to D90 <3μm, add it into the solvent, add emulsifier, cosolvent and other additives while stirring, and finally add it into water and disperse it evenly at a high speed of more than 8000rpm to form a uniform liquid.

[0089] 3. Field trials

[0090] Field efficacy test of a combination of pyraclostrobin and zoxanil against tobacco black shank.

[0091] (1) Test drug

[0092] Test group: the preparations prepared in Example 4 and Examples 10 to 16 (all of which had a compound ratio of pyraclostrobin to zoxamide of 5:4).

[0093] Control group: Comparative Example 1 and Comparative Example 2.

[0094] (2) Experimental arrangements

[0095] The test site was arranged in a diseased tobacco field in Lichuan City, Hubei Province, which was in the field growth period. This test adopted a random block arrangement, with each area of ​​25m 2 The spraying method was used for application, and the application was repeated 3 times in each area, with an interval of 7 days between each application. The therapeutic effects of the combination of pyraclostrobin and zoxystrobin with different active ingredient amounts on tobacco black shank were investigated. The test results are shown in Table 3.

[0096] Table 3 The control effect of different compound preparations on tobacco black shank

[0097] Group preparation Active ingredient dosage (g.ai / hectare) Treatment Effects Example 10 18% pyraclostrobin·zoxanil microcapsule suspension 10(pyraclostrobin)+8(zoxamide) 74.1％ Example 12 27% pyraclostrobin·zoxanil oil-based suspension emulsion 15(pyraclostrobin)+12(zoxamide) 79.8％ Example 16 27% pyraclostrobin·zoxanil microemulsion 15(pyraclostrobin)+12(zoxamide) 80.3％ Example 4 36% pyraclostrobin·zoxanil suspension concentrate 20(pyraclostrobin)+16(zoxamide) 86.8％ Embodiment 15 36% pyraclostrobin·zoxanil emulsion in water 20(pyraclostrobin)+16(zoxamide) 84.4％ Embodiment 11 45% pyraclostrobin·zoxanil wettable powder 25 (pyraclostrobin) + 20 (zoxamide) 90.5％ Embodiment 14 45% pyraclostrobin·zoxanil granules 25 (pyraclostrobin) + 20 (zoxamide) 88.1％ Example 13 54% pyraclostrobin·zoxanil water dispersible granules 25(pyraclostrobin)+24(zoxamide) 88.6％ Comparative Example 1 36% pyraclostrobin suspension 36(pyraclostrobin) 52.7％ Comparative Example 2 36% Benzamide SC 36(Benzamide) 36.8％

[0098] From Table 3 we can see that:

[0099] (1) The combination of pyraclostrobin and oxazolidinone has better therapeutic effect on tobacco black shank than the single preparations of either.

[0100] (2) In compound preparations, as the proportion of the total amount of active ingredients in the preparation increases, the control effect is significantly improved; however, when the total amount of active ingredients exceeds 45%, the control effect is not significantly improved; therefore, from the perspective of cost-effectiveness, the compounding ratio of pyraclostrobin to zoxadifen is 5:4 and the total amount of the two accounts for 25% to 45% of the preparation, which is the best choice.

[0101] (3) Examples 12 and 16 are both preparations with a total active ingredient content of 27%, Examples 4 and 15 are both preparations with a total active ingredient content of 36%, and Examples 11 and 14 are both preparations with a total active ingredient content of 45%. From the results of the prevention effect, the three groups of data are compared horizontally, and the prevention effect of the oil-based suspoemulsion, suspension concentrate and wettable powder is better. The inventor believes that the oil-based suspoemulsion and suspension concentrate can achieve a certain long-term sustained release effect of the active ingredient; the wettable powder also reaches a state similar to the suspension concentrate after being diluted with water, and can also achieve a certain long-term sustained release effect.

Claims

1. A pesticide composition of pyraclostrobin and zoxadiazole, characterized in that: The pesticide composition comprises pyraclostrobin, zoxanil and auxiliary materials; the mass ratio of pyraclostrobin to zoxanil is (1-50):(1-50); the total amount of pyraclostrobin and zoxanil accounts for 1% to 90% of the mass percentage of the pesticide composition.

2. The pesticide composition of pyraclostrobin and zoxadiazole according to claim 1, characterized in that: The ratio of the pyraclostrobin to the zoxadiazole is (1-50):(1-40).

3. The pesticide composition of pyraclostrobin and zoxadiazole according to claim 1, characterized in that: The ratio of the pyraclostrobin to the oxazolidinone is (1-40):(10-30).

4. The pesticide composition of pyraclostrobin and zoxadiazole according to claim 1, characterized in that: The ratio of the pyraclostrobin to the oxazolidinone is (1-30):(1-20).

5. The pesticide composition of pyraclostrobin and zoxamidopropynil according to claim 1, characterized in that: The total amount of the pyraclostrobin and the zoxadiazole accounts for 10% to 65% of the mass percentage of the pesticide composition.

6. The pesticide composition of pyraclostrobin and zoxadiazole according to claim 1, characterized in that: The compounding ratio of the pyraclostrobin and the zoxadiazole is 5:4, and the total amount of the two accounts for 27% to 45% of the preparation amount.

7. The pesticide composition of pyraclostrobin and zoxystrobin according to claim 1, characterized in that: The dosage forms of the pesticide composition include microcapsule suspension, suspension, wettable powder, oil-based suspoemulsion, water emulsion, microemulsion, water-dispersible granule and granule.

8. The pesticide composition of pyraclostrobin and zoxamidopropynil according to claim 7, characterized in that: When the pesticide composition is made into a microcapsule suspension, it includes the following components and contents: 1% to 50% of pyraclostrobin, 1% to 50% of oxazolidinone, 1% to 10% of a polymer capsule wall material, 2% to 10% of a dispersant, 1% to 10% of an organic solvent, 1% to 10% of an emulsifier, 0.1% to 5% of a pH regulator, 0.01% to 2% of a defoamer, 0.1% to 8% of an antifreeze agent, and the balance is deionized water; When the pesticide composition is made into a suspension, it includes the following components and contents: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 12% of a dispersant, 1% to 10% of a wetting agent, 0.1% to 12% of a thickener, 0.1% to 8% of an antifreeze agent, and the balance is deionized water; When the pesticide composition is made into a wettable powder, it includes the following components and contents: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 10% of a dispersant, 1% to 8% of a wetting agent, 0.1% to 5% of a stabilizer, and the remainder is filler; When the pesticide composition is made into an oil-based suspoemulsion, it includes the following components and contents: 1% to 50% of pyraclostrobin, 1% to 50% of oxazolidinone, 1% to 50% of oil, 1% to 8% of emulsifier, 1% to 15% of dispersant, 1% to 8% of antifreeze agent, 0.1% to 5% of stabilizer, and the balance is deionized water; When the pesticide composition is made into an aqueous emulsion, it includes the following components and contents: 1% to 50% of pyraclostrobin, 1% to 50% of oxazolidinone, 1% to 60% of an organic solvent, 1% to 12% of an emulsifier, 0.1% to 8% of an antifreeze agent, 0.01% to 2% of a defoamer, 0.1% to 2% of a thickener, and the balance is deionized water; When the pesticide composition is made into a microemulsion, it includes the following components and contents: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 25% of solvent, 10% to 35% of emulsifier, 5% to 15% of cosolvent, 3% to 5% of antifreeze agent, and the balance is deionized water; When the pesticide composition is made into water-dispersible granules, it includes the following components and contents: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 12% of dispersant, 1% to 8% of wetting agent, 1% to 10% of disintegrant, and the balance is filler; When the pesticide composition is made into granules, it comprises the following components and contents: 1% to 50% of pyraclostrobin, 1% to 50% of zoxamide, 1% to 12% of dispersant, 1% to 10% of wetting agent, 0.1% to 8% of binder, and the balance is filler.

9. A pesticide composition of pyraclostrobin and zoxamidopropynil according to any one of claims 1 to 8, characterized in that: The pesticide composition is used to prevent and treat crop diseases caused by fungal infection.

10. The pesticide composition of pyraclostrobin and zoxamidopropynil according to claim 9, characterized in that: The diseases caused by fungal infection include tobacco black shank.

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