Method for recommending dose of mutation breeding of oxytropis myriophyllum through neutron irradiation

Abstract

The invention discloses an oxytropis myriophyllum mutagenesis breeding dose recommendation method through neutron irradiation, and belongs to the technical field of mutagenesis breeding. The invention relates to an oxytropis myriophyllum mutagenesis breeding irradiation recommended dose method, which comprises the following steps: carrying out seed irradiation treatment by using different gradient radiation doses to obtain M1-generation seeds, and evaluating the germination rate, the emergence rate and the seedling growth condition of the M1-generation seeds to obtain the oxytropis myriophyllum mutagenesis breeding irradiation recommended dose. The recommended doses comprise 6.29 Gy, 4.62 Gy, 3.54 Gy, 2.80 Gy and 2.27 Gy, and the recommended doses comprise 6.29 Gy, 4.62 Gy, 3.54 Gy, 2.80 Gy and 2.27 Gy. The method provided by the invention can effectively improve the breeding efficiency of oxytropis myriophyllum.

Description

Technical Field

[0001] The invention relates to the technical field of mutation breeding, in particular to a method for recommending the dosage of neutron irradiation for mutation breeding of Oxytropis multifolia. Background Art

[0002] Oxytropis myriophylla Pall.DC. is a perennial herb of the genus Oxytropis in the Leguminosae family. It is widely used in traditional herbal medicine, and its dried aerial parts are often used to treat diseases such as plague, rashes and erysipelas. The plant is rich in chemical components such as flavonoids, saponins, alkaloids and phenolic glycosides, and exhibits pharmacological properties such as antioxidant, anti-inflammatory and antibacterial properties, and has important medicinal value. In addition to its medicinal value, Oxytropis myriophylla also has excellent ecological adaptability, especially in terms of barrenness and drought resistance. It is an important gene resource for genetic engineering breeding of high-quality forage in the future. Due to its strong ecological adaptability, Oxytropis myriophylla can grow under a variety of environmental conditions, and play an ecological role in improving soil structure and preventing soil erosion, helping to maintain the stability of the ecosystem.

[0003] Most of the innovation of germplasm resources is based on conventional methods, which have long cycles, low efficiency, and very limited types of mutations. Neutron radiation-induced mutagenesis breeding and the selection of crop varieties in combination with other technologies have become important breeding methods. However, for a long time, radiation-induced mutagenesis breeding has always been relatively blind, and it is necessary to find the recommended dose of radiation mutagenesis through a large workload. Therefore, how to effectively improve the breeding efficiency of multi-leaf thorn beans is of great research significance. Summary of the invention

[0004] The purpose of the present invention is to provide a method for providing a recommended dosage of neutron irradiation for mutation breeding of Oxytropis multifolia to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] Technical solution 1: A recommended irradiation dose for mutation breeding of Oxytropis multifolia, wherein the recommended irradiation dose includes 2.27Gy-6.29Gy.

[0007] Furthermore, the recommended irradiation doses include 6.29 Gy, 4.62 Gy, 3.54 Gy, 2.80 Gy and 2.27 Gy.

[0008] Furthermore, the optimal irradiation dose among the recommended irradiation doses is 6.29 Gy.

[0009] Technical solution 2: A method for obtaining the recommended radiation dose for induced breeding of multi-leafed thorn beans, comprising the following steps: irradiating seeds with different gradient radiation doses to obtain M 1 Generation of seeds, evaluation of M1 The seed germination rate, germination rate and seedling growth of the first generation were used to obtain the recommended irradiation dose for mutagenesis breeding of Oxytropis multifolia.

[0010] Furthermore, the recommended doses include 6.29 Gy, 4.62 Gy, 3.54 Gy, 2.80 Gy and 2.27 Gy.

[0011] Furthermore, the radiation type of the irradiation treatment includes neutron radiation.

[0012] The present invention discloses the following technical effects:

[0013] The present invention provides a method for obtaining a recommended radiation dose for crop mutation breeding, comprising irradiating seeds with different gradient radiation doses to obtain a recommended radiation dose for crop mutation breeding. 1 Generation of seeds, evaluation of M 1 The best irradiation dose was obtained by comparing the seed germination rate, germination rate and seedling growth of the first generation. The recommended irradiation doses included 6.29 Gy, 4.62 Gy, 3.54 Gy, 2.80 Gy and 2.27 Gy, among which the best irradiation dose was 6.29 Gy. The embodiment of the present invention verifies that the recommended dose provided in the present application can effectively improve the breeding efficiency of multi-leaf thorn bean. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 The effect of neutron irradiation on the root length and shoot length of Oxytropis multifolia seeds;

[0016] Figure 2 The effect of neutron irradiation on the root diameter of Oxytropis multifolia seeds;

[0017] Figure 3 These are improved Oxytropis multifolia induced by irradiation under different conditions. DETAILED DESCRIPTION

[0018] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0019] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0020] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0021] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0022] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0023] Example 1

[0024] (1) Effects of neutron irradiation on seed germination of Oxytropis multifolia

[0025] After neutron irradiation, the seed germination indexes showed no significant difference between different doses (P>0.05), as shown in Table 1. After irradiation with different doses, the germination rate, germination index and average germination days were promoted to varying degrees. When the dose was 6.29GY, the germination rate was 99%, the germination index was 14%, and the germination period was 2d. Its germination rate was basically the same as that of the control, but its germination index was higher than that of the control, and the average germination days were shorter than that of the control.

[0026] Table 1 Effects of neutron irradiation on germination parameters of Oxytropis multifolia seeds

[0027]

[0028] Note: The data are tabulated using mean ± standard error. Different lowercase letters indicate significant differences (P < 0.05), the same below.

[0029] (2) Effects of neutron irradiation on root and shoot length of Oxytropis multifolia seeds

[0030] The results of the effects of neutron irradiation on the root length and shoot length of Oxytropis multifolia seeds are shown in Figure 1 . When the neutron irradiation dose was 6.29 (GY), the root length of Oxytropis multifolia seeds became longer than that of the control group. When the irradiation dose was 4.63 (GY), 3.54 (GY), 2.8 (GY), and 2.27 (GY), the root length became shorter than that of the control group. This indicates that a neutron irradiation dose of 6.29 (GY) promotes the growth of the root of Oxytropis multifolia seeds. After irradiation at different doses, the shoot length became longer than that of the control group, and the shoot length was most significantly promoted when the irradiation dose was 3.54 (GY). This indicates that neutron irradiation has a promoting effect on the growth of its root length and shoot length, and the promoting effect on the shoot length is more significant. Figure 1 The effect of neutron irradiation on the root length and shoot length of Oxytropis multifolia seeds.

[0031] (3) Effects of neutron irradiation on root diameter of Oxytropis multifolia seeds

[0032] The results of the effects of neutron irradiation on the root diameter of Oxytropis multifolia seeds are shown in Figure 2 When the neutron irradiation dose was 6.29 (GY), 4.63 (GY), 3.54 (GY), and 2.8 (GY), the root diameter of Oxytropis multifolia seeds became thicker than that of the control group, and when the irradiation dose was 2.27 (GY), the root diameter became thinner than that of the control group. This indicates that the neutron irradiation dose of 2.27 (GY) has an inhibitory effect on the growth of the root diameter of Oxytropis multifolia seeds, while other doses have a promoting effect, and the most significant effect is when the dose is 6.29 (GY).

[0033] In summary, the seeds of Oxytropis multifolia were irradiated with neutron source to absorbed doses of 6.29, 4.62, 3.54, 2.80, and 2.27 Gy. The effects of absorbed dose on physiological parameters of Oxytropis multifolia seeds, such as germination potential, germination rate, bud length, root length, and root number, were observed. After irradiation with different doses, the germination rate, germination index, and average germination days were promoted to varying degrees. The results showed that when the dose was 6.29 GY, the germination rate was 99%, the germination index was 14%, and the average germination days was 2 days. Compared with the control, the germination rate was consistent, while the germination index was higher than the control, and the average germination days were shorter than the control. The highest irradiation treatment dose (6.29 Gy) was recommended as the dose for mutation breeding.

[0034] Specifically, the modified Oxytropis multifolia M was irradiated with a dose of 2.27-6.29 Gy. 0The seeds of the first generation were planted in the fields of Kezuohou Banner, Tongliao City. The germination rate was counted after sowing to obtain the recommended irradiation dose; the seeds of multi-leaf thorn bean were irradiated with a neutron source to an absorbed dose of 6.29, 4.62, 3.54, 2.80, and 2.27 Gy. The absorbed dose was observed to affect the germination rate of multi-leaf thorn bean seeds after sowing. The results showed that the germination rate was 87.8% when the dose was 3.54 Gy, and 87.4% when the dose was 6.29 Gy. The irradiation treatment dose with a high germination rate is used as the recommended dose for mutation breeding, and the recommended doses are 3.54 Gy and 6.29 Gy.

[0035] Table 2 Effects of neutron irradiation on germination of Oxytropis multifolia seeds

[0036]

[0037] Note: The table is based on mean ± standard error, and different lowercase letters indicate significant differences (P < 0.05).

[0038] Sowing M 0 Generate seeds, record agronomic traits, select individual plants with target traits, and harvest seeds to obtain M 1 Generation of seeds. Figure 3 The modified Oxytropis multifolia M was irradiated with a dose of 2.27-6.29 Gy. 0 The results showed that the germination rates of the 6.29Gy and 3.54Gy treatment groups were similar compared with the control group. Growth analysis showed that the treatments of 2.27Gy to 6.29Gy significantly promoted root growth, with the 6.29Gy treatment having the most significant effect. In addition, the treatment groups (2.27Gy to 6.29Gy) also showed a significant advantage in promoting bud growth compared with the control group. Overall, the 6.29Gy treatment had the best effect.

[0039] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A recommended irradiation dose for mutation breeding of Oxytropis multifolia, characterized in that: The recommended irradiation dose includes 2.27Gy-6.29Gy.

2. The recommended irradiation dose according to claim 1, characterized in that: The recommended irradiation doses include 6.29 Gy, 4.62 Gy, 3.54 Gy, 2.80 Gy and 2.27 Gy.

3. The recommended irradiation dose according to claim 1, characterized in that: The recommended irradiation dose is 6.29 Gy.

4. A method for obtaining the recommended radiation dose for induced breeding of Oxytropis multifolia, characterized in that: The following steps are involved: The seeds were irradiated with different gradient radiation doses to obtain M1 generation seeds, and the seed germination rate, germination rate and seedling growth of the M1 generation were evaluated to obtain the recommended irradiation dose for the multi-leaf oxtera bean mutation breeding.

5. The method according to claim 4, characterized in that The recommended doses include 6.29 Gy, 4.62 Gy, 3.54 Gy, 2.80 Gy and 2.27 Gy.

6. The method according to claim 4, characterized in that The type of radiation used in the irradiation treatment includes neutron radiation.

Images