A plant-based whitening and sunscreen composition, and its preparation method and application

Abstract

The present invention discloses a plant whitening sunscreen composition and its preparation method and application, and relates to the field of cosmetic technology. The present invention provides a plant whitening sunscreen composition, comprising the following components in parts by weight: 0.5-5 parts of a P / O / W composite carrier, 0.1-3 parts of bakuchiol, and 0.1-3 parts of a thyme extract. The present invention stabilizes two components, pterostilbene and verbascoside, in a P / O / W pickering multiple emulsion system to form a P / O / W composite carrier, which is mixed with bakuchiol and thyme extract in specific parts by weight. On the one hand, it can achieve safe and efficient whitening by repairing, inhibiting melanin production, inhibiting melanin transport, and promoting the synergistic enhancement of multiple pathways of melanin autophagy; on the other hand, it can achieve safe and efficient whitening and sun protection, achieve the effect of "external protection and internal whitening", and can synergistically strengthen the skin's defensive barrier and improve problems such as dull skin in sensitive skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a plant-based whitening and sunscreen composition, and a preparation method and application thereof. Background Art

[0002] People with sensitive skin have a thinner stratum corneum, which can easily impair the epidermal barrier function and lead to skin allergic reactions under physiological or pathological conditions. Ultraviolet radiation and inflammation are the primary factors that activate melanocytes to produce melanin. Because of this impaired skin barrier, sensitive skin is more susceptible to UV sensitization and dark spots than normal skin, necessitating greater attention to sun protection and whitening. Sunscreens and whitening agents in cosmetics are two ingredients with considerable safety controversy, and sensitive skin is more susceptible to irritation from these agents, leading to adverse reactions.

[0003] Current whitening sunscreens have the following shortcomings: (1) Current whitening sunscreen technologies mostly focus on the combination of whitening agents and the combination of sunscreens. They fail to consider the characteristics of sensitive skin, focusing on efficacy while ignoring safety. (2) For people with sensitive skin, simply combining whitening and sunscreen is not enough. The damaged barrier must be repaired first, and the skin's defenses must be strengthened before both whitening and sun protection can be achieved. (3) In research on whitening sunscreens for sensitive skin, few people have considered the synergistic effect of combining the two.

[0004] Therefore, how to provide a plant-based whitening and sunscreen composition that reduces the irritation of the formula while achieving effective sun protection and whitening effects through ingredient screening and reasonable compatibility has broad application prospects. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a plant-based whitening sunscreen composition that achieves effective sun protection and whitening effects while reducing the irritation of the formula through ingredient screening and reasonable compatibility, which has broad application prospects.

[0006] To achieve the above-mentioned object, the technical solution adopted by the present invention is as follows: a plant whitening sunscreen composition, comprising the following components in parts by weight: 0.5-5 parts of a P / O / W composite carrier, 0.1-3 parts of bakuchiol, and 0.1-3 parts of a thyme extract; the P / O / W composite carrier comprises three phases in the following mass percentages: 8-20% of an alcohol phase, 20-35% of an oil phase, and 50-70% of an aqueous phase; the alcohol phase comprises verbascoside, pterostilbene, and a polyol; the oil phase comprises an emulsifier and oil; The aqueous phase includes solid particles and deionized water; wherein the polyol includes at least two of butylene glycol, glycerol, dipropylene glycol, propylene glycol, and PEG400; the oil includes at least one of butyl octanol salicylate, C12-15 alcohol benzoate, isononyl isononanoate, and dicaprylyl carbonate; and the solid particles are at least one of cellulose and modified starch; and the mass percentage of verbascoside in the P / O / W composite carrier is 0.1-2%, and the mass percentage of pterostilbene is 0.1-5%.

[0007] The present invention stabilizes pterostilbene and verbascoside in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite encapsulation carrier. Firstly, pterostilbene and verbascoside have excellent activity but poor stability, especially the worse stability under light conditions. The present invention stabilizes pterostilbene and verbascoside in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite encapsulation carrier, which can better dissolve pterostilbene and verbascoside, and at the same time, together with bakuchiol and thyme extract in specific weight parts, can effectively block the influence of light on the stability of these components, thereby maintaining the biological activity of each component to the maximum extent. Secondly, the encapsulation form of the P / O / W composite encapsulation carrier solves the defects existing in the application of the components, such as poor stability and low activity in actual application, thereby improving the utilization rate and being more suitable for sensitive skin. At the same time, the present invention mixes a P / O / W composite carrier encapsulating pterostilbene and verbascoside with specific weight portions of bakuchiol and thyme extract, which can achieve safe and efficient whitening by repairing, inhibiting melanin production, inhibiting melanin transport, and promoting melanin autophagy through multi-pathway synergistic synergy; it can also achieve safe and efficient whitening and sun protection, achieving the effect of "external protection and internal whitening", and can synergistically strengthen the skin's defensive barrier and improve problems such as dull skin in sensitive skin. Thirdly, the plant whitening and sunscreen composition formed by the encapsulation of the specific P / O / W composite carrier of the present invention, combined with specific weight portions of bakuchiol and thyme extract, can achieve long-lasting efficacy after transdermal absorption during the action process.

[0008] Preferably, the weight ratio of the P / O / W composite carrier, bakuchiol, and thyme extract is P / O / W composite carrier: bakuchiol: thyme extract = 1: (0.1-0.8): (0.1-0.8).

[0009] Preferably, in the plant whitening and sunscreen composition, the mass percentage of the P / O / W composite carrier is 45-60%.

[0010] During actual experiments, the inventors found that the weight of the components in the plant whitening and sunscreen composition will affect the performance of the product. When the weight of the components is further selected within the above range, the obtained product has better whitening and sunscreen effects.

[0011] Preferably, the polyol is a mixture of glycerol and dipropylene glycol, and the weight ratio of the glycerol to dipropylene glycol is (2-8): (2-8).

[0012] Preferably, the dielectric constant of the grease is 2-4.

[0013] Further preferably, the polyol is a mixture of glycerol and dipropylene glycol, the weight ratio of the glycerol to dipropylene glycol is 1:1, and the oil is isononyl isononanoate.

[0014] The inventors found in actual experiments that the selection of the above-mentioned specific polyols and oils can better prepare the P / O / W composite packaging carrier, taking into account both the utilization of the active ingredients and the stability of the composite packaging carrier.

[0015] Preferably, the method for preparing the P / O / W composite carrier comprises the following steps:

[0016] S1, dissolving verbascoside and pterostilbene in polyol to prepare an inner alcohol phase;

[0017] S2, adding an emulsifier to the oil to prepare an oil phase;

[0018] S3, adding the alcohol phase to the oil phase and homogenizing and dispersing the mixture to obtain P / O colostrum;

[0019] S4, adding the solid particles into deionized water to disperse to obtain an aqueous phase;

[0020] S5. Add the P / O colostrum to the aqueous phase and disperse homogeneously to obtain a P / O / W composite carrier.

[0021] Preferably, in step S1, the weight ratio of verbascoside, pterostilbene and polyol is verbascoside: pterostilbene: polyol = (0.1-2): (0.1-5): 10;

[0022] And / or, in step S2, the weight ratio of oil and emulsifier is oil:emulsifier = (1-10):1; and / or, in step S3, the weight ratio of inner alcohol phase and oil phase is inner alcohol phase:oil phase = (2-4):(6-8); and / or, in step S4, the weight ratio of solid particles and deionized water is solid particles:deionized water = 1:(10-30); and / or, in step S5, the weight ratio of P / O colostrum and aqueous phase is P / O colostrum:aqueous phase = (2-5):(5-8).

[0023] Preferably, in step S2, the emulsifier is a polyglycerol emulsifier, and preferably, the polyglycerol emulsifier is at least one of polyglyceryl-3 diisostearate, polyglyceryl-2 triisostearate, and polyglyceryl-2 dipolyhydroxystearate.

[0024] In addition, the present invention provides the use of the plant whitening and sunscreen composition in the preparation of skin products.

[0025] Furthermore, the present invention provides a skin product, which includes the plant whitening and sunscreen composition; the skin product includes one of sunscreen lotion, sunscreen lotion, sunscreen cream, sunscreen liquid, sunscreen powder, sunscreen spray, sunscreen gel, isolation cream, isolation lotion, isolation lotion, and BB cream.

[0026] Preferably, the skin product comprises the following components in percentage by mass: 0.03-13% of a plant whitening and sunscreen composition, 2-30% of a chemical sunscreen and / or an inorganic UV blocker, 5-40% of a cosmetic base, and the balance being water.

[0027] Preferably, the chemical sunscreen is at least one of bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl triazone, polysilicone-15, methylene bis-benzotriazolyl tetramethylbutylphenol, terephthalate dicamphorsulfonic acid, drometrizole trisiloxane, ethylhexyl methoxycinnamate, and ethylhexyl salicylate; and the inorganic UV blocker is at least one of zinc oxide and titanium dioxide.

[0028] Preferably, the cosmetic base includes at least one of a thickener, a moisturizer, an emulsifier, a preservative, a fragrance, and a pH adjuster. Optionally, the thickener includes at least one of carbomer, xanthan gum, acrylates / C10-30 alkyl acrylate crosspolymer, and sodium polyacrylate; the moisturizer includes at least one of trehalose, sodium hyaluronate, erythritol, glycereth-26, xylitol glucoside, tremella extract, and β-glucan; the emulsifier includes at least one of hydrogenated lecithin, cetearyl glucoside, ceteareth-20, polyglyceryl-10 stearate, and ceteareth-20; the pH adjuster includes at least one of arginine, triethanolamine, and sodium hydroxide; and the preservative is at least one of phenoxyethanol, sodium benzoate, and a moisturizer with preservative properties such as p-hydroxyacetophenone and capryloylhydroxyvaleric acid.

[0029] Preferably, the present invention provides a whitening sunscreen lotion / cream comprising the following components in percentage by mass:

[0030] Deionized water balance, carbomer 0.1-0.5%, disodium EDTA 0.01-0.05%, butylene glycol 1-5%;

[0031] MONTANOV L (C14-22 alcohol, C12-20 alkyl glucoside) 1-5%, cetearyl alcohol 0.5-1.5%, diisopropyl sebacate 3-8%, dicaprylyl carbonate 3-8%, isononyl isononanoate 1-5%, bis-ethylhexyloxyphenol methoxyphenyl triazine 3-8%, ethylhexyl triazone 1-5%, drometrizole trisiloxane 1-5%, polysilicone-15 1-5%, titanium dioxide 1-3%;

[0032] 0.03-13% of plant whitening and sunscreen composition; 0.05-0.5% of essence; and 0.5-1% of preservative.

[0033] Optionally, the preservative is at least one of phenoxyethanol and sodium benzoate.

[0034] The present invention also provides a method for preparing the whitening sunscreen lotion / cream, comprising the following steps:

[0035] (1) Mix deionized water, carbomer, disodium EDTA, and butylene glycol, heat to 75-85°C, and stir until completely dissolved;

[0036] (2) Mix MONTANOV L (C14-22 alcohol, C12-20 alkyl glucoside), cetearyl alcohol, diisopropyl sebacate, dicaprylyl carbonate, isononyl isononanoate, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl triazone, drometrizole trisiloxane, polysilicone-15, and titanium dioxide, heat to 75-85°C, and stir until completely dissolved;

[0037] (3) Add the components in step (1) to the components in step (2), homogenize for 5-20 minutes, cool to 40-45° C., add the plant whitening sunscreen composition, essence and preservative, stir and cool to below 38° C. to obtain the whitening sunscreen lotion / cream.

[0038] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention stabilizes pterostilbene and verbascoside in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite encapsulation carrier. On the one hand, pterostilbene and verbascoside have good activity, but poor stability, especially the stability is even worse under light conditions; the present invention stabilizes pterostilbene and verbascoside in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite encapsulation carrier, which can better dissolve pterostilbene and verbascoside, and at the same time, together with a specific weight portion of bakuchiol and thyme extract, can effectively block the influence of light on the stability of these components, thereby maintaining the biological activity of each component to the maximum extent; on the other hand, the encapsulation form of the P / O / W composite encapsulation carrier solves the defects existing in the application of the components, such as poor stability and low activity in actual application, thereby improving the utilization rate and being more suitable for sensitive skin. At the same time, the present invention mixes a P / O / W composite carrier encapsulating pterostilbene and verbascoside with specific weight portions of bakuchiol and thyme extract, which can achieve safe and efficient whitening by repairing, inhibiting melanin production, inhibiting melanin transport, and promoting melanin autophagy through multi-pathway synergistic synergy; it can also achieve safe and efficient whitening and sun protection, achieving the effect of "external protection and internal whitening", and can synergistically strengthen the skin's defensive barrier and improve problems such as dull skin in sensitive skin. Thirdly, the plant whitening and sunscreen composition formed by the encapsulation of the specific P / O / W composite carrier of the present invention, combined with specific weight portions of bakuchiol and thyme extract, can achieve long-lasting efficacy after transdermal absorption during the action process. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is the HPLC standard curve of pterostilbene;

[0040] Figure 2 HPLC chart of the content of W3 in the pterostilbene P / O / W composite carrier;

[0041] Figure 3 This is the HPLC chart of the W2 content in the pterostilbene P / O / W composite encapsulation carrier. DETAILED DESCRIPTION

[0042] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to the accompanying drawings and specific examples. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0043] The raw materials used in the examples and comparative examples are described below, but are not limited to these materials:

[0044] Polyol-1: a mixture of glycerol and dipropylene glycol, with a weight ratio of glycerol to dipropylene glycol = 1:1;

[0045] Polyol-2: a mixture of glycerol and dipropylene glycol, with a weight ratio of glycerol to dipropylene glycol of 2:8;

[0046] Polyol-3: a mixture of glycerol and dipropylene glycol, with a weight ratio of glycerol to dipropylene glycol = 8:2;

[0047] Polyol-4: a mixture of glycerol and dipropylene glycol, with a weight ratio of glycerol to dipropylene glycol of 1:9;

[0048] Polyol-5: Glycerin;

[0049] Polyol-6: Butanediol;

[0050] Oil-1: Isononyl Isononanoate

[0051] Oil-2: C12-15 Alkyl Benzoate

[0052] Grease-3: White oil (<2)

[0053] Oil-4: Castor oil (>4)

[0054] Bakuchiol: Purchased from Guangdong Yuewei Microbiology Technology Co., Ltd.

[0055] Thyme extract: Japan Ichimaru Natural Beauty Co., Ltd.

[0056] Verbascoside: Shanghai source leaf;

[0057] Pterostilbene: Sabinsa (Nanjing) Co., Ltd.;

[0058] Examples 1-12 and Comparative Examples 1-9

[0059] The components and weight proportions of the P / O / W composite carrier of the present invention are shown in Table 1 below. The preparation method of the P / O / W composite carrier of the present invention comprises the following steps:

[0060] S1, dissolving verbascoside and pterostilbene in polyol to prepare an inner alcohol phase;

[0061] S2, adding an emulsifier to the oil to prepare an oil phase;

[0062] S3, adding the alcohol phase to the oil phase and homogenizing and dispersing the mixture to obtain P / O colostrum;

[0063] S4, adding the solid particles into deionized water to disperse to obtain an aqueous phase;

[0064] S5. Add the P / O colostrum to the aqueous phase and disperse homogeneously to obtain a P / O / W composite carrier.

[0065] Among them, the emulsifier used in the preparation method of the P / O / W composite carrier of the present invention is polyglycerol-3 diisostearate, and the solid particles used are octenyl succinic anhydride modified starch. PC / PURE, the emulsifier and the solid particles of the present invention are conventionally selected and can be conventionally selected by those skilled in the art.

[0066] Among them, the P / O / W composite package carrier-1 in Table 1 is omitted in the table and filled in as P / O / W-1. Similarly, the total weight parts of the P / O / W composite package carrier in Table 1 is 100 parts.

[0067] This application provides a botanical whitening sunscreen composition. The components and weight percentages of the botanical whitening sunscreen composition are shown in Tables 2 and 3 below. The botanical whitening sunscreen composition is prepared by uniformly mixing a P / O / W composite carrier, bakuchiol, and thyme extract to obtain the botanical whitening sunscreen composition. When preparing the comparative example botanical whitening sunscreen composition, if the relevant components are not present, omit them. If other components are present, add them together and mix thoroughly.

[0068] Table 1

[0069]

[0070] Table 2

[0071]

[0072]

[0073] Table 3

[0074]

[0075] Comparative Example 10

[0076] A plant-based whitening and sunscreen composition comprising the following components in parts by weight: 0.03 parts of pterostilbene, 0.06 parts of verbascoside, 1 part of bakuchiol, and 1 part of thyme extract;

[0077] The preparation method of the plant whitening and sunscreen composition comprises the following steps: uniformly mixing pterostilbene, verbascoside, bakuchiol and thyme extract to obtain the plant whitening and sunscreen composition.

[0078] Application Examples 1-13, Comparative Application Examples 1-10, Blank Application Examples

[0079] Application Examples 1-13 of the present invention, Comparative Application Examples 1-10 and Blank Application Examples provide a whitening sunscreen lotion / cream, the components (mass percentage) of the whitening sunscreen lotion / cream are shown in Table 4; wherein the plant whitening sunscreen compositions in Application Examples 1-12 are respectively the plant whitening sunscreen compositions prepared in Examples 1-12, and the plant whitening sunscreen compositions in Comparative Application Examples 1-10 are respectively the plant whitening sunscreen compositions prepared in Comparative Examples 1-10; for example, the plant whitening sunscreen composition used in Application Example 1 is the plant whitening sunscreen composition prepared in Example 1, the plant whitening sunscreen composition used in Application Example 2 is the plant whitening sunscreen composition prepared in Example 2, the plant whitening sunscreen composition used in Comparative Application Example 1 is the plant whitening sunscreen composition prepared in Comparative Example 1, and the same applies to the others.

[0080] The plant-based whitening and sunscreen composition used in Application Example 13 is the plant-based whitening and sunscreen composition prepared in Example 1.

[0081] Table 4

[0082]

[0083]

[0084] Performance Test-1 Safety Evaluation.

[0085] Test method: human patch test

[0086] Testing process: 30 volunteers were recruited and the safety of the product was tested using a qualified spot tester in accordance with the methods specified in the "Technical Specifications for Safety of Cosmetics".

[0087] A closed patch test method was used, and equal amounts (0.020 mL - 0.025 mL) of test samples (i.e., Application Examples 1-13, Comparative Application Examples 1-10, and Blank Application Examples) were placed in a specific patch tester, which was applied to the volunteer's arm with a low-allergenic tape. Light pressure was applied to evenly adhere the sample to the skin for 24 hours. After 24 hours, the patch tester was removed, and the skin reaction was observed after 0.5 hours, 24 hours, and 48 hours. The 48-hour skin results were recorded. The levels of adverse skin reactions are shown in Table 5 below, and the test results are shown in Table 6 below.

[0088] Table 5

[0089]

[0090] Table 6

[0091]

[0092]

[0093] The results of human patch tests show that the whitening sunscreen lotions / creams provided by Application Examples 1-13, Comparative Application Examples 1-10 and Blank Application Examples of the present invention are safe for use on human skin.

[0094] Performance Test-2 Sunscreen Performance Test

[0095] Using a sunlight simulator, a sun protection test was conducted on 5 volunteers according to the sun protection index determination method in the "Technical Specifications for Safety of Cosmetics".

[0096] Specifically: Determine the MED value of the volunteers in advance. After the back is divided into sections, one section is not coated with the sample, and the rest of the sections are coated with (2.00±0.05) mg / cm 2 Apply the sample with a fingertip and wait 30 minutes. Then, irradiate the sample with a solar simulator and record the MED value after application. Calculate the SPF by comparing the MED of the applied sample with the MED of the unapplied sample.

[0097] The following are the test results, as shown in Table 7.

[0098] Table 7

[0099]

[0100]

[0101] As can be seen from the above table, the sunscreen product prepared using the whitening sunscreen composition prepared by the embodiment of the present invention has an excellent SPF value of more than 30 and has a good sun protection effect.

[0102] Performance Test-3 Encapsulation Efficiency Test

[0103] Testing Procedure: Draw an HPLC standard curve for pterostilbene. Chromatographic conditions were a C18 column, a mobile phase of 65% potassium dihydrogen phosphate (10 mmol / L) in water + 35% acetonitrile, a flow rate of 0.8 ml / min, and a detection wavelength of 306 nm. The standard curve: y = 7.6789x + 0.9313. Pterostilbene showed good linearity between 1 and 100 ppm.

[0104] Accurately measure 1 ml of each of the P / O / W composite encapsulation vectors AK prepared in the present invention. Centrifuge (10,000 rpm for 15 min). The bottom liquid was collected and measured using HPLC to determine the free pterostilbene content, which was recorded as W2. After soaking in 5 ml of DMSO solution, the solution was ultrasonically disrupted and filtered, and the pterostilbene content was measured using HPLC to determine the free pterostilbene content, which was recorded as W3.

[0105] Encapsulation efficiency = (W3-W2) / W3*100%.

[0106] in, Figure 1 This is the HPLC standard curve of pterostilbene; Figure 2 HPLC chart of the content of W3 in the pterostilbene P / O / W composite carrier; Figure 3 This is the HPLC chart of the W2 content in the pterostilbene P / O / W composite encapsulation carrier.

[0107] The following are the encapsulation efficiency test results. In the table, P / O / W composite carrier-1 is omitted and filled in as P / O / W-1, and so on, as shown in Table 8:

[0108] Table 8

[0109]

[0110]

[0111] As can be seen from the above table, the encapsulation efficiency of the P / O / W composite carrier of the present invention is above 70%, which has a very good encapsulation effect.

[0112] Performance Test-4: Tyrosinase Inhibition

[0113] Tyrosinase inhibition was determined using an in vitro mushroom tyrosinase assay as follows:

[0114] 1. Solution preparation: (1) 100U / mL tyrosinase solution: dilute 25KU tyrosinase with PBS buffer to a 25mL brown volumetric flask, dilute to the mark, and dispense into 5 5mL centrifuge tubes and store in a -14℃ refrigerator. When using, take 1mL of 1000U tyrosinase solution and dilute 10 times to obtain 100U tyrosinase solution; (2) 5% L-dopa solution: accurately weigh 0.0125g of levodopa, place it in a 25mL brown volumetric flask, and dilute to the mark with pH 7.4 PBS buffer. (3) Test sample preparation: dissolve the sample in DMSO at a ratio of 1:1, and then filter it with a filter membrane to obtain the test sample.

[0115] Test: Prepare five sets of sample tubes and a blank control tube using the solutions in tubes A, B, C, and D as shown below. Add 0.5 mL of 5% L-DOPA solution to each tube. Incubate at 37°C for 30 minutes. Measure the absorbance of the mixed solution at 475 nm. Repeat three times for each sample.

[0116] The solutions in test tubes A, B, C, and D are as follows: Tube A: 3 mL PBS buffer + 0.5 mL tyrosinase; Tube B: 3.5 mL PBS buffer; Tube C: 1 mL sample solution + 2 mL PBS buffer + 0.5 mL tyrosinase; Tube D: 1 mL sample solution + 2.5 mL PBS buffer.

[0117] Data analysis: SPSS 20.0 and Excel 2020 were used for statistical analysis. The data were first tested for normal distribution using the single-sample "KS" test and homogeneity of variance. When the data were normally distributed, independent sample t-tests were used for two groups of data, and single-factor ANOVA analysis of variance was performed for three or more groups of data. The data were expressed as the mean ± standard deviation of three independent repeated data samples. For statistical differences between groups, the LSD test was used if the variance was not equal. If the variance was not equal, the Dunnett's T3 test was used to test the data. P < 0.05 was considered statistically significant. When the data did not conform to the normal distribution, a nonparametric test was used to analyze the data, and the data were expressed as M (P25-P75). GraphPad Prism 8.0 software was used to create all data graphs and calculate IC50. The smaller the IC50 value, the stronger the inhibitory ability against tyrosinase. The tyrosinase inhibition rate was calculated as [1-(CD) / (AB)] × 100%

[0118] Where:

[0119] C—absorbance value of the sample tube, i.e., absorbance value of the solution after the sample reacts with tyrosinase;

[0120] D—sample background absorbance;

[0121] A—Absorbance of enzyme reaction tube, i.e. absorbance of tyrosinase and DOPA reaction without adding sample;

[0122] B—solvent background absorbance;

[0123] The test results are shown in Table 9 below: Tyrosinase inhibition rate

[0124] Table 9

[0125]

[0126] It can be seen from the above table that the whitening and sunscreen composition prepared in the embodiment of the present invention has a tyrosinase inhibition rate of more than 80%.

[0127] Performance Test-5 Repair Barrier Test

[0128] Filaggrin (FLG): A water-soluble, acidic / neutral protein found in human epidermal keratinocytes (KCs). It is a matrix protein that forms the intercellular filaments of the eukaryotic cytoskeleton and participates in the formation of the intercellular corneum (CE) during KC terminal differentiation. Its precursor, profilaggrin, is dephosphorylated by caspase-14 to become filaggrin, which is then hydrolyzed into various natural skin barrier factors that maintain the water content of the stratum corneum, contribute to skin hydration, and safeguard the skin barrier. Filaggrin can also trigger KC apoptosis, causing KCs to transform from nucleated granular cells to anucleated corneocytes. When keratinocytes are damaged, filaggrin gene expression decreases, and the resulting decrease in filaggrin levels impairs the skin barrier. This patent uses UV stimulation of keratinocytes to simulate skin damage. Comparison of FLG gene expression is used to characterize the skin barrier. Comparison of FLG gene expression before and after application of a sample is used to assess the skin's repair capacity.

[0129] Specifically, HaCaT cells were plated on a 6-well plate, cultured for 24 h, the culture medium was discarded, and the cells were washed with PBS. The blank group was not irradiated with UVB, while the model group (irradiated with UVB) and the experimental group (irradiated with UVB + test sample) were exposed to 80 mJ / cm 2 The cells were irradiated with UVB. Complete culture medium containing various concentrations of the test samples was then added. After 48 hours, total RNA was extracted using a TRIzon, and RNA purity and concentration were determined using a NanoDrop. cDNA was then synthesized and amplified by PCR. The presence of target gene bands was confirmed by agarose gel electrophoresis. Finally, the cDNA was used for real-time PCR to quantitatively analyze the effects of the test samples on the expression of genes related to the skin barrier in UV-damaged HaCaT cells.

[0130] The experimental results are as follows: The expression levels of FLG gene in different samples are shown in Table 10.

[0131] Table 10

[0132] project FLG expression project FLG expression project FLG expression Blank group 100±7.46 Example 9 117.00±4.49* Comparative Example 7 70.2±12.45 Model Group 65.67±4.31### Example 10 151.33±13.33* Comparative Example 8 69.8±5.12 Example 1 185.6±23.50*** Example 11 126.00±6.93* Comparative Example 9 69.08±6.97 Example 2 175.33±13.81*** Example 12 121.33±13.33* Comparative Example 10 73.12±17.21 Example 3 177.67±11.63*** Comparative Example 1 82.33±4.58* - - Example 4 160.33±10.40*** Comparative Example 2 82.33±4.58* - - Example 5 120.33±11.26*** Comparative Example 3 95.2±4.03* - - Example 6 119.33±2.54*** Comparative Example 4 85.4±7.24* - - Example 7 138.67±9.99** Comparative Example 5 71.67±6.20 - - Example 8 134.33±11.03** Comparative Example 6 67.33±0.27 - -

[0133] Note: ### represents p<0.001 between the model group and the blank group, * represents p<0.05 between the embodiment and the model group, ** represents p<0.01 between the embodiment and the model group, and *** represents p<0.001 between the embodiment and the model group.

[0134] As can be seen from the above table, the whitening sunscreen composition prepared in the embodiment of the present invention has an FLG gene expression level of more than 100, and has a good skin barrier repair effect.

[0135] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A plant-based whitening and sunscreen composition, characterized in that: The invention comprises the following components in parts by weight: 0.5-5 parts of a P / O / W composite carrier, 0.1-3 parts of bakuchiol, and 0.1-3 parts of a thyme extract; the P / O / W composite carrier comprises three phases in the following mass percentages: an alcohol phase of 8-20%, an oil phase of 20-35%, and an aqueous phase of 50-70%; the alcohol phase comprises verbascoside, pterostilbene, and a polyol; the oil phase comprises an emulsifier and oil; and the aqueous phase comprises solid particles and deionized water; wherein the polyol The invention relates to a mixture of glycerol and dipropylene glycol, wherein the weight ratio of the glycerol to the dipropylene glycol is (2-8): (2-8); the oil is C12-15 alcohol benzoate or isononyl isononanoate; and the solid particles are at least one of cellulose and modified starch. The mass percentage of verbascoside in the P / O / W composite encapsulation carrier is 0.1-2%, and the mass percentage of pterostilbene is 0.1-5%. The preparation method of the P / O / W composite encapsulation carrier comprises the following steps: S1, dissolving verbascoside and pterostilbene in polyol to prepare an inner alcohol phase; S2, adding an emulsifier to the oil to prepare an oil phase; S3, adding the alcohol phase to the oil phase and homogenizing and dispersing the mixture to obtain P / O colostrum; S4, adding the solid particles into deionized water to disperse to obtain an aqueous phase; S5. Add the P / O colostrum to the aqueous phase and disperse homogeneously to obtain a P / O / W composite carrier.

2. The plant whitening and sunscreen composition according to claim 1, wherein: The weight ratio of the P / O / W composite carrier, bakuchiol, and thyme extract is P / O / W composite carrier: bakuchiol: thyme extract = 1: (0.1-0.8): (0.1-0.8).

3. The plant whitening and sunscreen composition according to claim 1, wherein In the plant whitening and sunscreen composition, the mass percentage of the P / O / W composite carrier is 45-60%.

4. The plant-based whitening and sunscreen composition according to claim 1, wherein: The weight ratio of the glycerol to the dipropylene glycol is 1:

1.

5. The plant whitening and sunscreen composition according to claim 1, wherein: In the step S1, the weight ratio of verbascoside, pterostilbene and polyol is verbascoside: pterostilbene: polyol = (0.1-2): (0.1-5): 10; And / or, in step S2, the weight ratio of oil to emulsifier is oil:emulsifier = (1-10):1; And / or, in step S3, the weight ratio of the inner alcohol phase to the oil phase is inner alcohol phase: oil phase = (2-4): (6-8); And / or, in step S4, the weight ratio of solid particles to deionized water is solid particles: deionized water = 1: (10-30); And / or, in step S5, the weight ratio of P / O colostrum to aqueous phase is P / O colostrum: aqueous phase = (2-5): (5-8).

6. Use of the plant whitening and sunscreen composition according to any one of claims 1 to 5 in the preparation of skin products.

7. A skin care product, characterized in that: The skin product includes the plant whitening sunscreen composition according to any one of claims 1 to 5; the skin product includes one of sunscreen milk, sunscreen lotion, sunscreen cream, sunscreen liquid, sunscreen powder, sunscreen spray, sunscreen gel, isolation cream, isolation milk, isolation lotion, and BB cream.

8. The skin care product according to claim 7, wherein The skin product comprises the following components in percentage by mass: 0.03-13% of the plant whitening sunscreen composition according to any one of claims 1 to 5, 2-30% of a chemical sunscreen and / or an inorganic UV blocker, 5-40% of a cosmetic base, and the balance being water; the cosmetic base comprises at least one of a thickener, a moisturizer, an emulsifier, a preservative, a fragrance, and a pH adjuster.

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