External composition with soothing and anti-inflammatory effects, preparation and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and provides an external composition with soothing and anti-inflammatory effects, a preparation and application thereof. The external composition is prepared from the following components in parts by weight: 4 to 5 parts of herba violae extract, 4 to 5 parts of chamomile flower extract, 1 to 3 parts of dandelion rhizome / root extract, 1 to 3 parts of Chinese herbaceous peony root extract, 1 to 3 parts of honeysuckle flower extract, 1 to 3 parts of mallow flower extract and 0.001 to 0.1 part of aloe barbadensis leaf extract; wherein the taraxacum vulgare rhizome / root extract is prepared by the following steps: smashing taraxacum vulgare rhizomes / roots, extracting with an ethanol-water solution, concentrating an extracting solution, carrying out enzymolysis with a compound enzyme preparation, and purifying with a macroporous adsorption resin column, so as to obtain the taraxacum vulgare rhizome / root extract. The content and the yield of the effective active ingredient total flavonoids in the taraxacum chinense rhizome / root extract in the external composition are high, and the composition has the advantages of being small in dosage and good in relieving and anti-inflammatory effect on allergic skin.

Description

Technical Field

[0001] The invention belongs to the technical field of functional health products, and relates to an external composition and preparation with soothing and anti-inflammatory effects and application thereof. Background Art

[0002] As the largest organ of the human body, the skin prevents the loss of water, electrolytes and other substances in the body, blocks the invasion of harmful substances from the outside, and maintains the stability of the human body's internal environment. However, with the widespread use of daily chemical products, more and more women have skin sensitivity problems. In addition, due to the influence of lifestyle, the probability of various allergens inducing immune allergic reactions such as dust mites, pollen, pet hair, and food additives is increasing. The incidence of allergic skin problems such as atopic dermatitis / eczema, allergic dermatitis, and sensitive skin has increased accordingly, attracting attention from all parties.

[0003] At present, Western medicine mainly adopts anti-allergic treatment, which has no radical curative effect, is prone to relapse and has more or less hormone components and side effects. Nowadays, people are more pursuing health and nature, and some active ingredients derived from nature are very popular, such as Chinese herbal medicine, plants, etc. For example, Chinese invention patent application CN102883732A discloses a composition comprising an Artemisia annua extract and an amine compound and a method for treating skin with the composition, and Chinese invention patent CN105213321B discloses the effects of Artemisia annua, artemisinin or Artemisia annua derivatives on atopic dermatitis / eczema, allergic asthma, allergic rhinitis, allergic dermatitis, sensitive skin, etc., but they all contain monomer compounds, the cost is increased and the effect needs to be further improved. Chinese invention patent application CN114732770 A discloses a composition used for soothing anti-skin allergy cosmetics, which contains chamomile, dandelion, aloe vera, mallow, peony root, honeysuckle, and Viola yedoensis. However, the applications all use the original plants as medicine with relatively large dosages, and the efficacy data are not disclosed. Its soothing and anti-inflammatory effects need to be further improved.

[0004] Therefore, there is an urgent need to provide a plant-derived external-use composition with a small dosage and good soothing and anti-inflammatory effects on allergic skin. Summary of the invention

[0005] The present invention aims to solve the problems in the prior art and provides a topical composition, preparation and application thereof with soothing and anti-inflammatory effects. By improving the preparation method of dandelion rhizome / root and leaf extract, the content and yield of the total flavonoids, an effective ingredient, are effectively increased. The composition composed of the obtained extract and other components has the characteristics of small dosage, good soothing and anti-inflammatory effects on allergic skin.

[0006] The present invention is achieved through the following technical solutions: The first aspect of the present invention is to provide a topical composition with soothing and anti-inflammatory effects, wherein the topical composition is composed by weight: 4-5 parts of Viola yedoensis extract, 4-5 parts of chamomile flower extract, 1-3 parts of dandelion rhizome / root extract, 1-3 parts of peony root extract, 1-3 parts of honeysuckle flower extract, 1-3 parts of mallow flower extract and 0.001-0.1 parts of aloe barbadensis leaf extract; Wherein, the dandelion rhizome / root extract is prepared by the following steps: (1) Extraction: crush the original medicinal material, rhizome / root of Taraxacum officinale, add it into ethanol-water solution for extraction, and obtain the rhizome / root extract of Taraxacum officinale; (2) Enzymatic hydrolysis: the dandelion rhizome / root extract is concentrated, ethanol is removed, diluted with water, placed in an enzymatic hydrolysis tank, and a composite enzyme preparation is added for enzymatic hydrolysis to obtain an enzymatic hydrolyzate; (3) Separation and purification: The enzymatic hydrolyzate is loaded onto a macroporous adsorption resin column, eluted with a water-ethanol system, and the eluate is concentrated and dried to obtain the dandelion rhizome / root leaf extract.

[0007] Preferably, in step (1), the concentration of the ethanol-water solution is 60-80% (V / V); and the mass volume ratio of the rhizome / root of Taraxacum officinale to the ethanol-water solution is 1:6-10 (g:mL).

[0008] Preferably, in step (1), the extraction temperature is 45-65°C, and the extraction is performed 2-3 times, each time for 20-30 minutes.

[0009] Preferably, in step (2), the composite enzyme preparation comprises 1-2 parts of cellulase, 0.05-0.2 parts of pectinase and 0.1-0.5 parts of β-glucosidase; the dosage of the composite enzyme preparation is 1-3wt.% of the rhizome / root of Taraxacum officinale; The enzymolysis temperature is 35-40°C, and the enzymolysis time is 60-80 minutes.

[0010] Preferably, in step (3), the filler of the macroporous adsorption resin column is a styrene-based macroporous adsorption resin, specifically selected from any one of HPD417, ADS-17, DM130 or HP-20.

[0011] Preferably, in step (3), the volume ratio of the enzymatic hydrolysate to the macroporous adsorption resin column filler is 4-9:1, and the elution is first washed with water and then gradient eluted with water-ethanol solutions of different concentrations.

[0012] Further preferably, the elution volume of water in the water wash is 3-5 BV, and the flow rate is 1-2 BV / h, preferably 2 BV / h.

[0013] Further preferably, the volume ratio of water-ethanol solutions of different concentrations used in the gradient elution is 90:10-10:90, the volume of the common eluent is 4-12BV, the flow rate is 1-2BV / h, preferably 1.5 BV / h, and the eluent of 40:60-20:80 is collected.

[0014] More preferably, the content of total flavonoids in the dandelion rhizome / root and leaf extract is ≥50%.

[0015] The second aspect of the present invention is to provide a preparation comprising the above-mentioned external composition.

[0016] Preferably, the preparation further comprises a pharmaceutically acceptable excipient.

[0017] Preferably, the preparation is in the form of an emulsion, a spray, an aerosol, a cream, an aqueous solution, a gel, an oil, a patch, a film or a mud.

[0018] The third aspect of the present invention is the use of the above-mentioned external composition or a preparation comprising the above-mentioned external composition in the preparation of cosmetics with soothing and anti-inflammatory effects.

[0019] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention significantly improves the content and yield of total flavonoids, an effective active ingredient in the rhizomes / roots of dandelions, by optimizing the enzymatic hydrolysis process of the rhizomes / roots of dandelions (including the selection of enzyme preparations and the regulation of dosage) and purification by macroporous adsorption resin columns; (2) The composition of the present invention has the characteristics of small dosage and good soothing and anti-inflammatory effects on allergic skin. DETAILED DESCRIPTION

[0020] It is worth noting that the raw materials used in the present invention, whose sources are not specifically limited, are all common commercially available products.

[0021] Embodiment 1: The dandelion rhizome / root extract is prepared by the following steps: (1) Crush the rhizome / root of European dandelion, add it to an ethanol-water solution (75% (V / V)) and extract it twice at 50°C. The solid-liquid ratio of the first extraction (European dandelion rhizome / root powder and ethanol-water solution) is 1:8 (g / mL). The extraction is carried out for 30 minutes. The residue is filtered and subjected to a second extraction with a solid-liquid ratio of 1:6 (g / mL). The extraction is carried out for 20 minutes. The filtrates are combined to obtain the European dandelion rhizome / root extract; (2) The extract of the rhizome / root of dandelion was concentrated, ethanol was removed, and the extract was diluted with water. The extract was placed in an enzymatic hydrolysis tank, and a composite enzyme preparation (cellulase, pectinase, β-glucosidase mass ratio of 1:0.1:0.25) with a dosage of 2 wt% of the rhizome / root of dandelion was added. The extract was enzymatically hydrolyzed at 35°C for 60 minutes to obtain an enzymatic hydrolyzate. (3) The hydrolyzate was centrifuged at 50,000 rpm for 5 min, and the supernatant was taken and passed through a macroporous adsorption resin column (HPD417). The volume ratio of the hydrolyzate to the macroporous adsorption resin column filler was 5:1. The supernatant was first eluted with 4 BV of water at a flow rate of 2 BV / h, and then eluted with a water-ethanol solution with a volume ratio of 90:10-10:90. The total volume of the eluent was 6 BV, and the flow rate was 1.5 BV / h. The eluate in the 40:60-20:80 portion was collected, concentrated, and dried to obtain the dandelion rhizome / root extract.

[0022] Embodiment 2: The dandelion rhizome / root extract is prepared by the following steps: (1) Crush the rhizome / root of European dandelion, add it to an ethanol-water solution (75% (V / V)) and extract it twice at 50°C. The solid-liquid ratio of the first extraction (European dandelion rhizome / root powder and ethanol-water solution) is 1:8 (g / mL). The extraction is carried out for 30 minutes. The residue is filtered and subjected to a second extraction with a solid-liquid ratio of 1:6 (g / mL). The extraction is carried out for 20 minutes. The filtrates are combined to obtain the European dandelion rhizome / root extract; (2) The extract of the rhizome / root of dandelion was concentrated, ethanol was removed, and the extract was diluted with water. The extract was placed in an enzymatic hydrolysis tank, and a composite enzyme preparation (cellulase, pectinase, β-glucosidase mass ratio of 2:0.05:0.5) with a dosage of 1 wt% of the rhizome / root of dandelion was added. The extract was enzymatically hydrolyzed at 35°C for 60 minutes to obtain an enzymatic hydrolyzate. (3) The hydrolyzate was centrifuged at 50,000 rpm for 5 min, and the supernatant was taken and passed through a macroporous adsorption resin column (ADS-17). The volume ratio of the hydrolyzate to the macroporous adsorption resin column filler was 5:1. The supernatant was first eluted with 4 BV of water at a flow rate of 2 BV / h, and then eluted with a water-ethanol solution with a volume ratio of 90:10-10:90. The total volume of the eluent was 6 BV, and the flow rate was 1.5 BV / h. The eluate in the 40:60-20:80 portion was collected, concentrated, and dried to obtain the dandelion rhizome / root extract.

[0023] Embodiment 3: The dandelion rhizome / root extract is prepared by the following steps: (1) Crush the rhizome / root of European dandelion, add it to an ethanol-water solution (75% (V / V)) and extract it twice at 50°C. The solid-liquid ratio of the first extraction (European dandelion rhizome / root powder and ethanol-water solution) is 1:8 (g / mL). The extraction is carried out for 30 minutes. The residue is filtered and subjected to a second extraction with a solid-liquid ratio of 1:6 (g / mL). The extraction is carried out for 20 minutes. The filtrates are combined to obtain the European dandelion rhizome / root extract; (2) The extract of the rhizome / root of dandelion was concentrated, ethanol was removed, and the extract was diluted with water. The extract was placed in an enzymatic hydrolysis tank, and a composite enzyme preparation (cellulase, pectinase, and β-glucosidase in a mass ratio of 1:0.2:0.1) with a dosage of 3 wt% of the rhizome / root of dandelion was added. The extract was enzymatically hydrolyzed at 35°C for 60 minutes to obtain an enzymatic hydrolyzate. (3) The hydrolyzate was centrifuged at 50,000 rpm for 5 min, and the supernatant was taken and passed through a macroporous adsorption resin column (DM130). The volume ratio of the hydrolyzate to the macroporous adsorption resin column filler was 5:1. The supernatant was first eluted with 4 BV of water at a flow rate of 2 BV / h, and then eluted with a water-ethanol solution with a volume ratio of 90:10-10:90. The total volume of the eluent was 6 BV, and the flow rate was 1.5 BV / h. The eluate in the 40:60-20:80 portion was collected, concentrated, and dried to obtain the dandelion rhizome / root extract.

[0024] Comparative Example 1: The only difference from Example 1 is that the complex enzyme preparation is replaced by cellulase and pectinase (mass ratio is 1:0.1), and the total amount used is the same as the total amount of the complex enzyme preparation in Example 1.

[0025] Comparative Example 2: The only difference from Example 1 is that the complex enzyme preparation is replaced by a single β-glucosidase, and the dosage is the same as the total dosage of the complex enzyme preparation in Example 1.

[0026] Comparative Example 3: The dandelion rhizome / root extract is prepared by the following steps: (1) Crush the rhizome / root of European dandelion, add it to an ethanol-water solution (75% (V / V)) and extract it twice at 50°C. The solid-liquid ratio of the first extraction (European dandelion rhizome / root powder and ethanol-water solution) is 1:8 (g / mL). The extraction is carried out for 30 minutes. The residue is filtered and subjected to a second extraction with a solid-liquid ratio of 1:6 (g / mL). The extraction is carried out for 20 minutes. The filtrates are combined to obtain the European dandelion rhizome / root extract; (2) The extract of the rhizome / root of dandelion was concentrated, ethanol was removed, and the extract was diluted with water. The extract was placed in an enzymatic hydrolysis tank, and a composite enzyme preparation (cellulase, pectinase, β-glucosidase mass ratio of 1:0.1:0.25) with a dosage of 2 wt% of the rhizome / root of dandelion was added. The extract was enzymatically hydrolyzed at 35°C for 60 minutes to obtain an enzymatic hydrolyzate. (3) Concentrating and drying the enzymatic hydrolyzate to obtain the dandelion rhizome / root extract.

[0027] The difference from Example 1 is that the enzymatic hydrolysate in step (3) is not purified by a macroporous resin column but is directly concentrated and dried.

[0028] Test Example 1: According to the method in the document "Liu Jia. Study on the Alleviating Effect of Total Flavonoids from Dandelion on Heat Stress in Caenorhabditis elegans [D]. Fuyang Normal University, 2019.", the total flavonoids content in the dandelion rhizome / root extracts prepared in the above-mentioned embodiments and comparative examples was determined, and the amount of total flavonoids in the dandelion rhizome / root was determined. The yield of total flavonoids was calculated based on the total flavonoids in the dandelion rhizome / root extracts. The results are shown in Table 1.

[0029] Table 1: Content and yield of total flavonoids in the rhizome / root extract of the present invention

[0030] Comparison of Comparative Examples 1 and 2 with Example 1 shows that: in the present invention, cellulase and pectinase as a whole work synergistically with β-glucosidase to significantly increase the content and yield of total flavonoids in the rhizome / root extract of dandelion.

[0031] Comparison of Example 3 with Example 1 shows that the macroporous resin chromatography column has a good separation and purification effect on the flavonoids in the enzymatic hydrolyzate, thereby increasing the content of total flavonoids in the dandelion rhizome / root extract. Under the premise of equal total flavonoids, the dosage of the dandelion rhizome / root extract obtained by the present invention is smaller.

[0032] Test Example 2: 1. Drug: The dandelion rhizome / root extract obtained in Example 1-3 and Comparative Example 1-3 is combined with other components of the present invention to form the composition of Example 1-3 and Comparative Example 1-3. The composition comprises: 4 parts of Viola yedoensis extract, 4 parts of chamomile flower extract, 3 parts of dandelion rhizome / root extract (Example 1-3, Comparative Example 1-3), 2 parts of peony root extract, 1 part of honeysuckle flower extract, 1 part of Malva sylvestris flower extract and 0.1 part of aloe vera leaf extract.

[0033] 2. Hacat cell plating Digestion of cells: UV irradiation for 20-30 minutes, spray paper towels with alcohol disinfection, wipe the clean bench. Take out the cell culture bottle, put it in the clean bench after disinfection, pour out the culture medium, wash twice with PBS buffer, pour out PBS, add 1mL TE buffer, after full contact, pour out a small amount of TE, digest in the incubator for 8-9 minutes, and observe under a microscope.

[0034] Stop digestion: After the cells are completely digested from the wall of the culture flask, add 5 mL of complete culture medium to stop digestion and resuspend the cell solution with a disposable dropper.

[0035] Cell counting: Count the cell stock solution, take 10 μL to one side of the counting plate, and 10 μL to the other side, count 2-3 times, take the average value, calculate the cell density, and calculate and prepare the cell solution according to the cell density and volume required for plating.

[0036] Plating: 200 μL of cell solution was added to each well of a 96-well plate, with a cell density of 2.0×10 5 The cells were cultured in an incubator at 37°C and 5% CO2 for 8-12 hours to allow the cells to adhere to the wall and grow.

[0037] 3. Cell loading (1) Washing the plate: Take out the 96-well plate, aspirate the culture medium with a pipette, and wash twice with 200 μL PBS.

[0038] (2) Sample loading: Set up a blank group, a model group, and a drug group. The blank group was inoculated with 200 μL of serum-free culture medium; the model group was inoculated with 200 μL of serum-free culture medium; the drug group was inoculated with 200 μL of different samples (the compositions of Examples 1-3 and Comparative Examples 1-3 were diluted with serum-free culture medium, respectively). After 2 hours, bacterial stimulation was added (cell: bacteria = 1:20), and the culture was continued for 8 hours.

[0039] 4. TNF-α ELISA kit detection The collected cell culture supernatant was tested according to the ELISA kit instructions. The specific results are shown in Table 2.

[0040] Table 2: TNF-α content detection experimental results of each group

[0041] Compared with the blank group, the model group ## =P < 0.01; compared with the model group, the drug group * indicates P < 0.05, ** indicates P < 0.01; compared with the composition of Example 1, the drug group & Indicates P < 0.05, && Indicates P < 0.01.

[0042] Compared with the blank group, the TNF-α content of Hacat cells was significantly upregulated after stimulation with Propionibacterium acnes, indicating that the modeling was successful. Compared with the model group, after adding the sample of the composition of Examples 1-3 and Comparative Examples 1-3, the TNF-α content of the cells was significantly reduced, wherein the composition of Examples 1-3 showed significant inhibition of TNF-α secretion, and the composition of Comparative Examples 1-3 had statistically significant inhibition of TNF-α secretion. Compared with the composition of Example 1, the inhibitory effect of the composition of Comparative Examples 1-3 on TNF-α secretion was lower than that of the composition of Example 1, wherein the effect of the composition of Comparative Examples 1 and 2 was significantly different from that of the composition of Example 1, and the effect of the composition of Comparative Example 3 was statistically significant compared with that of the composition of Example 1. Therefore, after optimizing the enzymatic hydrolysis process of dandelion rhizomes / roots (including the selection of enzyme preparations and dosage regulation) and purifying with a macroporous adsorption resin column, the composition composed of dandelion rhizomes / root extracts and other components had the best inhibitory effect on TNF-α secretion, showing a good anti-inflammatory effect.

[0043] Test Example 3: The compositions of Examples 1-3 and Comparative Examples 1-3 in Test Example 2 were prepared into 10% aqueous solutions with deionized water as test sample solutions.

[0044] In this experiment, SD rats were selected and randomly divided into a blank group, a model group, an embodiment 1-3 group, and a comparative example 1-3 group, with 10 rats in each group. The animals were depilated 24 hours before the experiment, and the back of the animals was depilated, with an area of ​​about 3cm×3cm. Except for the blank group, the other groups used 2,4-dinitrochlorobenzene (DNCB) to model the back skin of the rats, and irradiated with ultraviolet rays for 12 hours. After the modeling was successful (the skin showed symptoms such as dryness, desquamation, burning, stinging, swelling, and erythema). The blank group and the model group were respectively coated with 0.5mL of deionized water on the depilated skin area on the back, with an application range of about 2.5cm×2.5cm. The other groups were respectively coated with 0.5mL of the test sample solution of different groups, and the drug was administered once a day for 21 consecutive days. On the 22nd day, the skin condition of the right back of each group of rats was observed with the naked eye, and recorded according to the test evaluation criteria in Table 3, and the concentrations of histamine (HIS) and immunoglobulin E (IgE) in the serum of each sample were detected.

[0045] Table 3: Skin reaction test criteria

[0046] The skin reactions and serum HIS and IgE concentrations of rats in each group are shown in Table 4.

[0047] Table 4: Skin reactions of rats in each group and the concentrations of HIS and IgE in serum

[0048] Compared with the blank group, the model group ## =P < 0.01; compared with the model group, the drug group * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001; compared with the composition of Example 1, the drug group & Indicates P < 0.05, && Indicates P < 0.01.

[0049] The allergic symptoms, serum HIS and IgE concentrations of the rats in the model group were significantly higher than those in the blank group, which was statistically significant. After applying the sample solutions of Examples 1-3 and Comparative Examples 1-3 of the present invention, the HIS and IgE concentrations were significantly lower than those in the model group, and the allergic symptoms were significantly relieved, almost returning to normal levels, indicating that the composition of the present invention has obvious anti-inflammatory and anti-irritation effects, and can well relieve allergic skin and skin dryness, desquamation, swelling, erythema and other symptoms caused by ultraviolet radiation. At the same time, the effect of the composition of Comparative Examples 1-3 on reducing the concentration of HIS and IgE and relieving allergic symptoms was significantly lower than that of the composition of Examples 1-3.

[0050] In summary, after optimization of the enzymatic hydrolysis process of dandelion rhizomes / roots (including the selection of enzyme preparations and dosage regulation) and purification by macroporous adsorption resin column, the composition of dandelion rhizomes / root extracts and other components has obvious anti-inflammatory and soothing effects.

[0051] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A topical composition with soothing and anti-inflammatory effects, characterized in that: The topical composition is composed of 4-5 parts of Viola yedoensis extract, 4-5 parts of chamomile flower extract, 1-3 parts of dandelion rhizome / root extract, 1-3 parts of peony root extract, 1-3 parts of honeysuckle flower extract, 1-3 parts of mallow flower extract and 0.001-0.1 parts of aloe barbadensis leaf extract by weight; Wherein, the dandelion rhizome / root extract is prepared by the following steps: (1) Extraction: crush the original medicinal material, rhizome / root of Taraxacum officinale, add ethanol-water solution for extraction, and filter the solution to obtain the rhizome / root extract of Taraxacum officinale; (2) Enzymatic hydrolysis: the dandelion rhizome / root extract is concentrated, ethanol is removed, diluted with water, placed in an enzymatic hydrolysis tank, and a composite enzyme preparation is added for enzymatic hydrolysis to obtain an enzymatic hydrolyzate; (3) Separation and purification: The enzymatic hydrolyzate is loaded onto a macroporous adsorption resin column, eluted with a mobile phase, and the eluate is concentrated and dried to obtain the dandelion rhizome / root and leaf extract.

2. The external use composition according to claim 1, characterized in that In step (1), the volume concentration of the ethanol-water solution is 60-80%; the mass volume ratio of dandelion rhizome / root to ethanol-water solution is 1 g: 6-10 mL.

3. The external use composition according to claim 1, characterized in that In step (1), the extraction temperature is 45-65°C, and the extraction is performed 2-3 times, each time for 20-30 minutes.

4. The external use composition according to claim 1, characterized in that In step (2), the composite enzyme preparation comprises 1-2 parts of cellulase, 0.05-0.2 parts of pectinase and 0.1-0.5 parts of β-glucosidase; the amount of the composite enzyme preparation is 1-3wt.% of the dandelion rhizome / root; The enzymolysis temperature is 35-40°C, and the enzymolysis time is 60-80min.

5. The external use composition according to claim 1, characterized in that In step (3), the filler of the macroporous adsorption resin column is a styrene-based macroporous adsorption resin, specifically selected from any one of HPD417, ADS-17, DM130 or HP-20.

6. The external use composition according to claim 1, characterized in that In step (3), the volume ratio of the enzymatic hydrolysate to the macroporous adsorption resin column filler is 4-9:1, and the elution is first washed with water, then gradient eluted with water-ethanol solutions of different concentrations, and the eluate is concentrated and dried.

7. The composition for external use according to claim 6, characterized in that The volume ratio of water-ethanol solutions of different concentrations used in the gradient elution is 90:10-10:90, the volume of the common eluent is 4-12 BV, and the eluent of 40:60-20:80 is collected.

8. The composition for external use according to claim 7, characterized in that The content of total flavonoids in the dandelion rhizome / root and leaf extract is ≥50%.

9. A preparation containing the external composition according to any one of claims 1 to 8, characterized in that: The dosage form of the preparation is one or more of emulsion, spray, aerosol, cream, water, gel, oil, patch, film or mud.

10. Use of the external-use composition according to any one of claims 1 to 8 or the preparation according to claim 9 in the preparation of cosmetics with soothing and anti-inflammatory effects.

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