An anti-aging composition for reducing structural dark circles and eye fine lines and its application

Abstract

The present invention discloses an anti-aging composition for reducing structural dark circles and fine lines around the eyes and its application, and relates to the field of cosmetic technology. The present invention provides an anti-aging composition comprising the following components in parts by weight: 0.01-0.2 parts of Ruscus aconitifolia extract, 0.01-1 parts of Coleus forskohlii extract, 0.01-2 parts of μ-conopeptide, and 0.01-3 parts of a fermentation product of Lactobacillus crispatus; the Ruscus aconitifolia extract comprises ruscosapogenin C 27 H 42 O4 and neoruscosaponin C 27 H 40 The anti-aging composition provided by the present invention, when the four components of Ruscus aconitifolia extract, Coleus forskohlii extract, μ-conopeptide, and Lactobacillus crispatus fermentation product are used synergistically, can enhance skin firmness and elasticity, effectively reduce structural dark circles and smooth fine lines around the eyes, and the effect can be immediately and continuously achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to an anti-aging composition for reducing structural dark circles and fine lines around the eyes and application thereof. Background Art

[0002] With the accelerated pace of modern life, staying up late, using electronic devices for extended periods, and overusing the eyes have become commonplace. These unhealthy habits cause the eye area to be the first part of the face to show signs of aging and experience the most severe symptoms, manifesting as structural dark circles, eye bags, and fine lines. Sensitive skin, due to its weak barrier function and susceptibility to irritation, is often more susceptible to inflammatory aging, exacerbating these problems. Therefore, developing eye care products tailored to sensitive skin is particularly important, meeting the dual needs of mildness and effectiveness.

[0003] Among existing technologies, medical aesthetic techniques such as laser treatment, liposuction surgery and injectable fillers have good effects on structural dark circles and eye bags, but surgery is risky, accompanied by side effects and is expensive; common eye cream active ingredients such as retinol and caffeine have anti-aging and eye puffiness improvement effects respectively, but the effects of these ingredients are often limited to the surface, making it difficult to deeply improve the problems of structural dark circles and fine lines, and some ingredients may irritate sensitive skin and cause allergic reactions.

[0004] Therefore, an anti-aging composition that enhances skin firmness and elasticity, effectively reduces structural dark circles and smoothes fine lines around the eyes, and enhances the overall youthfulness of the eye skin has broad application prospects. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an anti-aging composition, preparation method and application thereof, which can enhance skin firmness and elasticity, effectively reduce structural dark circles and smooth fine lines around the eyes, and enhance the overall youthfulness of the eye skin.

[0006] To achieve the above object, the technical solution adopted by the present invention is: an anti-aging composition, comprising the following components in parts by weight: 0.01-0.2 parts of Ruscus aconitifolia extract, 0.01-1 parts of Coleus forskohlii extract, 0.01-2 parts of μ-conopeptide, and 0.01-3 parts of Lactobacillus crispatus fermentation product; the Ruscus aconitifolia extract includes ruscosapogenin C 27 H 42 O4 and neoruscosaponin C 27 H 40 O4.

[0007] The butcher's vine extract has significant anti-inflammatory, antibacterial and capillary permeability-reducing effects, can reduce capillary fluid extravasation, and thus alleviate edema; the butcher's vine extract selected by the present invention includes at least one of butcher's vine (root) extract, butcher's vine (rhizome) extract, and butcher's vine (leaf) extract; the main active ingredient of the Coleus forskohlii extract is forskolin, which can stimulate the activity of adenylate cyclase and increase the level of cellular cyclic adenosine monophosphate (cAMP), thereby promoting local fat metabolism and improving the appearance of eye bags already formed in the lower eyelid; μ-conopeptide is a bioactive peptide extracted from cone snails (Ipomoea), which can block muscle nerve conduction, thereby enhancing skin firmness and reducing wrinkles; the Lactobacillus crispatus is a specific bacterium living in the crow's feet area and disappears on aging skin over time. Its fermentation product promotes skin hydration, reduces skin dryness and sagging, can effectively enhance skin elasticity, and has a significant effect on improving fine lines around the eyes.

[0008] The inventors of the present application found in actual experiments that when the four components of Butcher's Pear extract, Coleus Forskohlii extract, μ-conopeptide, and Lactobacillus crispatus fermentation product are used synergistically, they can synergistically enhance skin firmness and elasticity, effectively lighten structural dark circles and smooth fine lines around the eyes, and enhance the overall youthfulness of the skin around the eyes.

[0009] Preferably, the anti-aging composition comprises the following components in parts by weight: 0.02-0.1 parts of Ruscus aconitifolia extract, 0.05-0.5 parts of Coleus forskohlii extract, 0.05-0.5 parts of μ-conopeptide, and 0.1-2 parts of Lactobacillus crispatus fermentation product.

[0010] Preferably, the composition comprises the following components in parts by weight: 0.05-0.07 parts of Ruscus aconitifolia extract, 0.2-0.3 parts of Coleus forskohlii extract, 0.1-0.2 parts of μ-conopeptide, and 0.5-1 parts of Lactobacillus crispatus fermentation product.

[0011] The inventors of this application found in actual experiments that the weight of the components in the anti-aging composition will affect the performance of the product. When the weight of the components is further selected to be within the above-mentioned range, the obtained product has a more significant effect of enhancing skin firmness and elasticity, fading structural dark circles and smoothing fine lines around the eyes.

[0012] Preferably, the weight ratio of ruscogenin to neoruscogenin in the Ruscus rubra extract is 1:(0.2-2.5). Further preferably, the weight ratio of ruscogenin to neoruscogenin in the Ruscus rubra extract is 1:(0.7-1.4); more preferably, the weight ratio of ruscogenin to neoruscogenin in the Ruscus rubra extract is 1:(0.9-1.2).

[0013] The inventors of the present application found in actual experiments that the weight ratio of ruscosaponin and neoruscosaponin in the Butcher's Pear extract will further affect the performance of the prepared anti-aging composition. When the weight ratio of ruscosaponin and neoruscosaponin is within the above range, the obtained product has a more significant effect of enhancing skin firmness and elasticity, fading structural dark circles and smoothing fine lines around the eyes.

[0014] Preferably, the preparation method of the Butcher's Broom extract is as follows:

[0015] (1) grinding and crushing the leaves and / or rhizomes and / or roots of Ruscus rubra and sieving to obtain a coarse powder, acid hydrolyzing and then reflux extraction, and filtering to obtain a filtrate;

[0016] (2) extracting the filtrate and then centrifuging it, collecting the organic phase, washing it with alkali, and drying it to powder to obtain the Ruscus aconitifolia extract.

[0017] Preferably, in step (1), the weight ratio of the Butcher's Wheat Leaf to the Butcher's Wheat Root is 1:(0.5-5); and / or the weight ratio of the Butcher's Wheat Leaf to the Butcher's Wheat Root is 1:(2-7).

[0018] Preferably, in the step (1), after grinding and pulverization, the product is sieved through an 80-200 mesh sieve, the solvent for acidic hydrolysis is at least one of an aqueous solution of hydrochloric acid / sulfuric acid and an alcoholic solution of hydrochloric acid / sulfuric acid, the acid concentration is 0.2-2.0 mol / L, and the mass ratio of the coarse powder to the solvent is 1 g: (40-60) mL; the temperature for reflux extraction is 70-90° C., and the time for reflux extraction is 2-4 h; in the step (2), the extraction solvent is at least one of n-hexane and dichloromethane, the volume ratio of the extraction solvent to the filtrate is (1-2): 1, the centrifugal speed is 1500-3000 rpm, and the centrifugal time is 1-5 min; the alkali for the alkali washing is sodium hydroxide, and the weight ratio of the alkali solution for the alkali washing to the organic phase is 1: (3-6); and the drying is carried out under a nitrogen environment at 50-65° C.

[0019] In addition, the present invention provides use of the anti-aging composition in preparing skin products.

[0020] Optionally, the skin product includes at least one of lotion, emulsion, cream, facial mask, essence, spray, and eye cream.

[0021] Furthermore, the present invention provides a skin product comprising the following components in mass percentage: 1-20% anti-aging composition, 5-40% cosmetic base, and the balance water; the cosmetic base comprises at least one of a thickener, a moisturizer, an emulsifier, a preservative, and a pH adjuster.

[0022] Optionally, the present invention provides an eye cream comprising the following components in percentage by weight: 1-20% anti-aging composition, 0.05-0.5% thickener, 0.5-2% moisturizer, 5-20% emulsifier, 0.5-3% preservative, 0.01-0.3% pH regulator, and the balance deionized water.

[0023] Optionally, the eye cream comprises at least one of the following (a)-(e):

[0024] (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0025] (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0026] (c) the pH adjusting agent comprises at least one of arginine, tromethamine, and EDTA-disodium;

[0027] (d) the emulsifier comprises at least one of caprylic capric triglyceride, hexyldecanol, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisostearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0028] (e) The preservative includes at least one of 1,3-propylene glycol, 1,2-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

[0029] Compared with the prior art, the present invention has the following beneficial effects: (1) The inventors of the present invention have found in actual experiments that when the four components of Ruscus aconitifolia extract, Coleus forskohlii extract, μ-cono peptide, and Lactobacillus crispatus fermentation product work together, they can synergistically enhance skin firmness and elasticity, effectively reduce structural dark circles and smooth fine lines around the eyes, and enhance the overall youthfulness of the skin around the eyes. (2) Ruscogenin and neoruscogenin in the Ruscus aconitifolia extract of the present invention reduce capillary permeability and edema, thereby instantly improving the volume of dark circles, while μ-cono peptide inhibits muscle nerve impulses, enhances skin firmness, and smoothes fine lines around the eyes, with an immediate effect. When the four components of Ruscus aconitifolia extract, Coleus forskohlii extract, μ-cono peptide, and Lactobacillus crispatus fermentation product work together, the effects of enhancing skin firmness and elasticity, effectively reducing structural dark circles and smoothing fine lines around the eyes can be immediately reflected and maintained. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 These are the dark circle improvement pictures of volunteers before using eye cream, 30 minutes after using eye cream, and 28 days after using eye cream for blank application example, application example 1, and application example 3; among them, (a) is the dark circle condition picture before use, (b) is the dark circle condition picture 30 minutes after using eye cream, and (c) is the dark circle condition picture 28 days after using eye cream. DETAILED DESCRIPTION

[0031] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to the accompanying drawings and specific examples. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0032] The raw materials used in the embodiments of the present invention are now further described, but are not limited to the following raw materials:

[0033] Coleus forskohlii flower extract: purchased from Shaanxi Ruilin Panier Biotechnology Co., Ltd.

[0034] μ-Conopeptide: purchased from Shenzhen Weiqi Biotechnology Co., Ltd.;

[0035] Lactobacillus crispatus fermentation product: purchased from BASF, Germany;

[0036] Commercially available butcher's nut extract: purchased from NORMACTIVE, Türkiye;

[0037] Aesculus hippocastanum extract: purchased from Xi'an Jinheng Chemical Co., Ltd.

[0038] Palmitoyl tetrapeptide-7: purchased from Guangzhou Baiyu Biotechnology Co., Ltd.

[0039] The filtrate of the fermentation product of bifid yeast was purchased from Guangzhou Baiyu Biotechnology Co., Ltd.

[0040] Butcher's Broom Extract-1

[0041] The preparation method of the Butcher's Broom extract-1 comprises the following steps:

[0042] (1) The leaves of the Ruscus aculeatus tree were ground and sieved through an 80-mesh sieve, and then subjected to reflux extraction after acid hydrolysis at a temperature of 80° C. for 3 h. The filtrate was obtained after filtration; the solvent for the acid hydrolysis was a 70% isopropanol solution containing 1 mol / L sulfuric acid, and the mass ratio of the crude powder to the solvent was 1 g:50 mL;

[0043] (2) extracting the filtrate and then centrifuging it, collecting the organic phase, washing it with an aqueous sodium hydroxide solution, and drying it to powder to obtain the Ruscus aconitifolia extract; the extraction solvent is n-hexane, the volume ratio of the extraction solvent to the filtrate is 1:1, the centrifugal speed is 2000 rpm, and the centrifugal time is 3 min; the weight ratio of the 1 mol / L aqueous sodium hydroxide solution to the organic phase is 1:5; and the drying is carried out in a nitrogen environment at 60°C.

[0044] Butcher's Broom Extract-2~Butcher's Broom Extract-9

[0045] The preparation methods of the Ruscus aconitifolia extracts-2 to -9 are compared with the preparation method of the Ruscus aconitifolia extract-1, except that the ratio of the raw materials in step (1) is different, as shown in Table 1 below, and the remaining components, weight parts and preparation methods are exactly the same.

[0046] Butcher's Broom Extract-10

[0047] The preparation method of the Butcher's Broom Extract-10 comprises the following steps:

[0048] (1) The leaves of the Ruscus aculeatus tree were ground and sieved through a 150-mesh sieve, and then subjected to reflux extraction after acid hydrolysis at a temperature of 70° C. for 4 h. The filtrate was obtained after filtration; the solvent for the acid hydrolysis was an aqueous solution containing 1 mol / L hydrochloric acid, and the mass ratio of the crude powder to the solvent was 1 g:60 mL;

[0049] (2) extracting the filtrate and then centrifuging it, collecting the organic phase, washing it with an aqueous sodium hydroxide solution, and drying it to powder to obtain the Ruscus aconitifolia extract; the extraction solvent is dichloromethane, the volume ratio of the extraction solvent to the filtrate is 2:1, the centrifugal speed is 3000 rpm, and the centrifugal time is 2 min; the weight ratio of the 0.5 mol / L aqueous sodium hydroxide solution to the organic phase is 1:3; and the drying is carried out under a nitrogen environment at 50°C.

[0050] In the Ruscus aconitifolia extract, the relationship between the raw materials and the weight ratio of ruscosapogenin and neoruscosapogenin in the Ruscus aconitifolia extract are shown in Table 1.

[0051] The assay method for ruscosapogenin and neoruscosapogenin in Ruscus rubra extract is as follows: 1 mg of Ruscosapogenin extract was accurately weighed and dissolved in 10 mL of methanol. After filtering through a 0.22 μm filter, the contents of ruscosapogenin and neoruscosapogenin in the extract were determined by high-performance liquid chromatography (HPLC), and the ratio was calculated. The chromatographic column was a C18 column (100×3.0 mm, 3.5 μm), the mobile phase was acetonitrile (70%): 0.1% formic acid in water (30%), the flow rate was 1 mL / min, the column temperature was 45°C, and the sample volume was 10 μL. The ratio of ruscosapogenin to neoruscosapogenin was calculated according to the following formula: Ratio = Ruscosapogenin Peak Area / Neoruscosapogenin Peak Area.

[0052] Table 1

[0053] Case Raw material (Ruscus aconite) Ruscogenin: Neoruscogenin Butcher's Broom Extract-1 blade 1:0.2 Butcher's Broom Extract-2 Leaf: rhizome = 1:0.5 1:0.7 Butcher's Broom Extract-3 Leaf: rhizome = 1:1 1:0.9 Butcher's Broom Extract-4 Leaves: rhizomes = 1:2 1:1.2 Butcher's Broom Extract-5 Leaf: rhizome = 1:5 1:1.4 Butcher's Broom Extract-6 rhizome 1:1.6 Butcher's Broom Extract-7 root 1:2.3 Butcher's Broom Extract-8 Leaf: root = 1:1 1：0.5 Butcher's Broom Extract-9 Root: rhizome = 1:1 1：1.7 Butcher's Broom Extract-10 blade 1:0.2 Commercially available butcher's clover extract - 1：1.8

[0054] Examples and Comparative Examples

[0055] The present invention provides an anti-aging composition. The components and weight portions of the anti-aging composition are shown in Table 2-3. The preparation method of the anti-aging composition comprises the following steps: dissolving a Ruscus aconitifolia extract and a Coleus forskohlii extract in 6 parts by weight of hexyldecanol, dissolving μ-conopeptide and a fermentation product of Lactobacillus crispatus in 4 parts by weight of water, and then uniformly mixing the two to obtain the anti-aging composition.

[0056] Table 2

[0057]

[0058]

[0059] Table 3

[0060]

[0061] Application Examples

[0062] The application examples of the present invention provide an eye cream, the components of the eye cream (mass percentage) are shown in Table 4; the compositions used in Application Examples 1-16 are the anti-aging compositions prepared in Examples 1-16 respectively; the anti-aging compositions used in Comparative Application Examples 1-6 are the anti-aging compositions prepared in Comparative Examples 1-6 respectively; for example, the anti-aging composition used in Application Example 1 is the anti-aging composition in Example 1, the anti-aging composition used in Application Example 5 is the anti-aging composition in Example 5, the anti-aging composition used in Application Example 15 is the anti-aging composition in Example 15, and so on; the anti-aging composition used in Application Examples 17-18 is the anti-aging composition prepared in Example 3.

[0063] Table 4

[0064]

[0065] The preparation method of the eye cream provided in Application Example 1 comprises the following steps:

[0066] (1) Mix the humectant and thickener with water, heat to 85±2°C, and homogenize at 1200 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated component A.

[0067] (2) Mix the emulsifier, heat to 85±2°C, and homogenize at 1200 rpm for 4 minutes. After homogenization, keep warm and set aside to obtain prefabricated component B;

[0068] (3) Mix the preservatives and heat to 60±2°C to melt to obtain prefabricated component C;

[0069] (4) Heat the preformed component A to 80±2°C, add the preformed component B at a speed of 250 rpm, stir and mix, then cool to 60±2°C, add the preformed component C at a speed of 250 rpm, stir and mix, then cool to 40°C, add the anti-aging composition and continue stirring for 8 minutes, finally add the remaining pH adjuster to adjust the pH to 6.0, then stop stirring, discharge the material, and obtain the eye cream.

[0070] The preparation methods of the remaining application examples are the same as that of application example 1, except that no relevant components are added.

[0071] Performance Test-1 Capillary Permeability Reduction Test

[0072] Experimental Methods: The cell line used was human skin capillary endothelial cells (BNCC337860, Beina Biosciences). The test conditions were: incubator temperature 37±1°C, saturated humidity, 5±1% carbon dioxide. Cells were cultured and treated according to grouping, and then tested. Specifically, the tight junction protein Claudin-1 content was measured using the following test method:

[0073] (1) The cell suspension was inoculated into a 96-well plate at a culture density of 2000 cells / well. 100 μL of endothelial cell culture medium (BNCC364799, Beina Biotech) was added to each well. After culturing for 24 hours, the supernatant was discarded.

[0074] (2) 100 μL of endothelial cell culture medium was added to the blank control group, and 100 μL of culture medium containing 0.1% of the anti-aging composition of the embodiment and the comparative example was added to the sample group. After culturing for 24 hours, the cells were collected and washed three times with PBS buffer. 50 μL of RIPA cell lysis buffer containing protease inhibitors was added to each well. The lysed samples were centrifuged at 12000 g for 5 minutes, and the supernatant was collected.

[0075] Determination of tight junction protein Claudin-1 content: The human claudin-1 ELISA kit (Cat. No. BLL106520E, Baililai Bio) was used to detect the Claudin-1 protein content in the cell supernatant of each group.

[0076] Tight junctions are the primary connection between capillary endothelial cells and regulate capillary permeability. Higher levels of tight junction protein Claudin-1 after sample treatment indicate lower capillary permeability and greater edema-reducing efficacy. The edema-reducing effect of the anti-aging composition is represented by the rate of increase in tight junction protein Claudin-1 in human capillary endothelial cells, using the following formula:

[0077] Claudin-1 increase rate = (Claudin-1 content in sample group / Claudin-1 content in blank group - 1) * 100%. The test results are shown in Table 5.

[0078] Table 5

[0079]

[0080]

[0081]

[0082] As can be seen from the above table, the anti-aging composition prepared in the embodiment of the present invention has a Claudin-1 increase rate of more than 50%, and has a good effect of reducing edema.

[0083] From the comparison of Examples 1-11, it can be seen that the weight ratio of ruscosapogenin and neoruscosapogenin in the Ruscus aconitifolia extract further affects the performance of the anti-aging composition prepared. When the weight ratio of ruscosapogenin and neoruscosapogenin is 1:(0.2-2.5), the Claudin-1 increase rate is 50%.

[0084] When the weight ratio of ruscosapogenin and neoruscosapogenin is 1: (0.9-1.2), the Claudin-1 increase rate is more than 80%.

[0085] From the comparison of Examples 12-16, it can be seen that the weight of the components in the anti-aging composition will affect the performance of the product. When the Ruscus aconitifolia extract is 0.05-0.07 parts, the Coleus forskohlii extract is 0.2-0.3 parts, and the μ

[0086] - 0.1-0.2 parts of conopeptide and 0.5-1 parts of fermentation product of Lactobacillus crispatus, the anti-aging composition has a better effect in reducing edema.

[0087] Performance Test-2 Human skin patch test.

[0088] Thirty volunteers (15 men and 15 women, aged 20-50 years) were recruited for a closed patch test. Equal amounts (0.020 mL to 0.025 mL) of the test sample (eye cream prepared in the application example, comparative application example, and blank application example) were placed in a specific patch tester. The patch was then applied to the volunteer's arm with hypoallergenic tape, gently pressed to evenly adhere to the skin, and left in place for 24 hours. A blank control group received distilled water. After 24 hours, the patch tester was removed, and skin reactions were observed 0.5, 24, and 48 hours later, with the results recorded. The severity of adverse skin reactions is shown in Table 6 below.

[0089] Table 6

[0090]

[0091]

[0092] After testing, the eye creams prepared in the application examples of the present invention, the comparative application examples and the blank application examples all showed negative reactions after human patch tests, indicating that they are safe and non-irritating to human skin.

[0093] Performance Test-3 Human Efficacy Test.

[0094] Asian adult test subjects aged 28-60 with sensitive skin and structural dark circles and fine lines around the eyes were randomly divided into 25 groups of 5 participants each. The volunteers gently applied the samples to their eyes once daily, morning and evening (applicable to eye creams prepared in Application Examples 1-18, Comparative Application Examples 1-6, and the blank application example). They were advised not to stay up late during use. Data were collected before use (T0), 30 minutes after use (T30min), and 28 days after use (T28).

[0095] After the visit, the volunteers washed their faces with facial cleanser and sat quietly in an air-conditioned room at a temperature of 21±1°C and a humidity of 50±10% for 2 hours. Their dark circles and fine lines were photographed and analyzed using VISIA-CR, and the fundus skin firmness parameter F4 and skin elasticity parameter R2 were measured using a Cutometer (MPA580, Courage and Khazaka, Germany).

[0096] The effect of reducing structural dark circles is represented by the improvement in the volume of dark circles, the effect of smoothing wrinkles is represented by the improvement in the area of ​​fine lines around the eyes, the effect of tightening the skin is represented by the improvement in the firmness F4 value, and the effect of increasing skin elasticity is represented by the improvement in the elasticity parameter R2 value. The formula is as follows:

[0097] Dark circle volume, eye fine lines area, F4 value improvement rate = (T0-Tx) / T0*100%;

[0098] R2 value improvement rate = (Tx-T0) / T0*100%;

[0099] The results are shown in Table 7.

[0100] Table 7

[0101]

[0102]

[0103] As can be seen from the above table, the eye cream prepared in the application example of the present invention has a good effect of enhancing skin firmness and elasticity, fading structural dark circles and smoothing fine lines around the eyes.

[0104] Figure 1 These are the dark circle improvement pictures of volunteers before using eye cream, 30 minutes after using eye cream, and 28 days after using eye cream for blank application example, application example 1, and application example 3; among them, (a) is the dark circle condition picture before use, (b) is the dark circle condition picture 30 minutes after using eye cream, and (c) is the dark circle condition picture 28 days after using eye cream.

[0105] From the comparison of Application Examples 1-11, it can be seen that the weight ratio of ruscosaponin and neoruscosaponin in the Butcher's Pear extract will further affect the performance of the prepared anti-aging composition. When the weight ratio of ruscosaponin and neoruscosaponin is 1: (0.9-1.2), the effect of enhancing skin firmness and elasticity, fading structural dark circles and smoothing fine lines around the eyes is better.

[0106] From the comparison of Application Examples 12-16, it can be seen that the weight parts of the components in the anti-aging composition will affect the performance of the product. When 0.05-0.07 parts of Ruscus aconitifolia extract, 0.2-0.3 parts of Coleus forskohlii extract, 0.1-0.2 parts of μ-cono peptide, and 0.5-1 parts of Lactobacillus crispatus fermentation product are used, the eye cream prepared can enhance skin firmness and elasticity, and has better effects in fading structural dark circles and smoothing fine lines around the eyes.

[0107] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-aging composition, characterized in that The invention comprises the following components in parts by weight: 0.01-0.2 parts of Ruscus aconitifolia extract, 0.01-1 parts of Coleus forskohlii extract, 0.01-2 parts of μ-conopeptide, and 0.01-3 parts of fermentation product of Lactobacillus crispatus; the Ruscus aconitifolia extract comprises ruscosaponin C 27 H 42 O4 and neoruscosaponin C 27 H 40 O4; in the Ruscus aconitifolia extract, the weight ratio of ruscosapogenin and neoruscosapogenin is 1:(0.7-1.4); the preparation method of the Ruscus aconitifolia extract is as follows: (1) Grinding and crushing the leaves and rhizomes of Ruscus rubra and sieving to obtain a coarse powder, acid hydrolyzing and reflux extraction, and filtering to obtain a filtrate; the weight ratio of the leaves and rhizomes of Ruscus rubra is 1: (0.5-5); (2) extracting the filtrate and then centrifuging it, collecting the organic phase, washing it with alkali, and drying it to powder to obtain the Ruscus aconitifolia extract; the extraction solvent is at least one of n-hexane and dichloromethane.

2. The anti-aging composition according to claim 1, wherein The invention comprises the following components in parts by weight: 0.02-0.1 parts of Ruscus aconitifolia extract, 0.05-0.5 parts of Coleus forskohlii extract, 0.05-0.5 parts of μ-cono peptide and 0.1-2 parts of fermentation product of Lactobacillus crispatus.

3. The anti-aging composition according to claim 1, wherein The invention comprises the following components in parts by weight: 0.05-0.07 parts of Ruscus aconitifolia extract, 0.2-0.3 parts of Coleus forskohlii extract, 0.1-0.2 parts of μ-cono peptide and 0.5-1 parts of fermentation product of Lactobacillus crispatus.

4. The anti-aging composition according to claim 1, wherein In the Butcher's Broom extract, the weight ratio of ruscosapogenin to neoruscosapogenin is 1:(0.9-1.2).

5. The anti-aging composition according to claim 1, wherein In the step (1), after grinding and pulverization, the powder is sieved through an 80-200 mesh sieve, and the mass ratio of the coarse powder to the solvent is 1 g: (40-60) mL; the temperature of the reflux extraction is 70-90° C., and the reflux extraction time is 2-4 h; in the step (2), the volume ratio of the extraction solvent to the filtrate is (1-2):1, the centrifugal speed is 1500-3000 rpm, and the centrifugal time is 1-5 min; the alkali for the alkali washing is sodium hydroxide, and the weight ratio of the alkali solution for the alkali washing to the organic phase is 1: (3-6); and the drying is carried out in a nitrogen environment at 50-65° C.

6. Use of the anti-aging composition according to any one of claims 1 to 5 in the preparation of skin products.

7. A skin care product, characterized in that: The skin care product comprises the following components in percentage by weight: 1-20% of the anti-aging composition according to any one of claims 1-5, 5-40% of a cosmetic base, and the balance water; the cosmetic base comprises at least one of a thickener, a humectant, an emulsifier, a preservative, and a pH adjuster.

Images