Hair collection and detection method

Abstract

The invention discloses a hair collection and detection method which comprises the following steps: S1, hair sample collection: cutting hair with the length of less than 3cm at the rear part of the head of an extracted person to obtain a to-be-detected hair sample; s2, hair sample treatment: putting a to-be-detected hair sample into a sampling tube filled with a grinding liquid, and grinding the to-be-detected hair sample; s3, hair sample detection: detecting and converting the content of drugs in the hair sample through a drug trace analyzer, the method is suitable for the technical field of in-vitro diagnosis, a systematic and complete hair collection and detection method is provided, evidence can be provided for abuse of drugs or not, and the taking type and time of the drugs can also be authenticated. The method can be applied to rapid on-site detection such as screening in entertainment venues and major activity venues, and can be used as a basis for drug taking behavior determination and drug taking addiction determination and a means for staged monitoring in community drug rehabilitation and social rehabilitation work.

Description

Technical Field

[0001] The invention belongs to the technical field of in vitro diagnosis, and in particular relates to a hair collection and detection method. Background Art

[0002] Drug abuse is a major problem that seriously endangers social security and human life and health. The number of drug users is increasing and they are getting younger. At present, immunocolloidal gold technology, gas chromatography-mass spectrometry chromatography and high performance liquid chromatography-mass spectrometry are mainly used at home and abroad to detect and identify blood, saliva, urine and hair samples of drug users. The detection window time for blood and saliva samples is 30 minutes to a few days, the detection window time for urine samples is 2 hours to 7 days, and the hair sample test can trace the drug history of 3 to 6 months. Usually 24 to 48 hours after taking drugs, the drugs in blood, urine and saliva are basically metabolized and excreted, resulting in negative tests and unable to provide valuable information.

[0003] Because hair follicles are surrounded by dense capillaries, drugs in the blood enter the hair follicles during the growth of hair. In about 3-5 days, hair containing drug molecules will grow out of the scalp surface and exist stably. Hair grows outward at a relatively constant speed, and the time, type and dosage of drug use can be inferred. Hair drug abuse detection has unique advantages such as easy sample acquisition and preservation, hygienic sampling process, high detection rate, long detection time, accurate test results, and can infer the drug use of the person being tested within a week to several years.

[0004] However, among existing drug tests, body fluid testing has become more mature, while hair testing still has some omissions. Existing drug tests lack a systematic and complete hair testing method. Summary of the invention

[0005] The purpose of the present invention is to overcome the defects of the prior art and provide a hair collection and detection method.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] A hair collection and detection method comprises the following steps:

[0008] S1 Hair sample collection: Cut the hair within 3 cm in length from the back of the head of the person to be tested to obtain the hair sample to be tested;

[0009] S2 Hair sample processing: Put the hair sample to be tested into a sampling tube filled with grinding liquid and grind it;

[0010] S3 Hair sample testing: Detect and convert the drug content in the hair sample through a drug trace analyzer.

[0011] Preferably, in step S1, at least two hair samples to be tested are collected, each weighing not less than 50 mg, wrapped in aluminum foil, and respectively placed in evidence bags, which are then sealed; the sample number, extraction date and extractor information are filled in the evidence bags, and the extracted person presses fingerprints and signs to confirm the seal of the evidence bag. If the extracted person refuses to press fingerprints or sign, the extractor shall indicate this and record the entire extraction process.

[0012] Preferably, the step S1 further comprises: preparing a hair sample extraction information table, recording the name of the person to be extracted, the resident identification number of the person to be extracted, the type of extracted hair, the extraction location, the extraction unit, the extraction personnel, and the extraction time.

[0013] Preferably, in step S1, hair from different people should be extracted separately, packaged independently, and numbered uniformly, and residues on the extraction equipment during the sampling process should be cleaned in time.

[0014] Preferably, in step S2, the grinding solution comprises 2 ml of hair lysis solution prepared by 0.5 mg / ml proteinase K, 0.61% tris; 0.85% NACL; 1.0% TWEEN-20; 0.1% Proclin 300 purified water and 95% zirconium oxide grinding beads with a particle size of 2-5 mm.

[0015] Preferably, in step S3, the hair sample detection comprises the following steps:

[0016] S31 turns on the drug trace analyzer, inserts the ID card corresponding to this batch of products and reads it;

[0017] S32 tear open the aluminum foil bag, take out the test card, and place it horizontally on the laboratory table;

[0018] S33 Click the test button on the main interface of the applicable instrument, then click the scan barcode button, align the barcode on the reagent card with the scanning area on the right side of the machine, and after the scan is successful, the interface jumps to the start test interface;

[0019] S34: taking the supernatant of the mixed solution of drug extraction in the processed hair, adding the sample and timing;

[0020] S35 uses a drug trace analyzer to detect and automatically convert the drug content in the hair sample.

[0021] Preferably, in step S34, the processed hair lysate in the sampling tube is sucked with a disposable pipette, 80uL is added to the sample well of the detection card, and the timing is started.

[0022] Preferably, in step S35, the added test card is reacted outside the machine for 5 minutes, inserted into the immunofluorescence analyzer, the screen test is clicked, and the instrument display screen is waited to display the test results and automatically print the test results.

[0023] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0024] The present invention provides a systematic and complete hair collection and detection method, which can provide evidence for drug abuse and verify the type and time of drug intake. It can be used for rapid on-site detection such as screening in entertainment venues and major event venues, as well as as a basis for drug abuse behavior identification and drug addiction identification, and as a means of phased monitoring in community drug rehabilitation and social rehabilitation work. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The present invention is a flow chart of a hair collection and detection method. DETAILED DESCRIPTION

[0026] The following is combined with Figure 1 , further illustrating a specific implementation of a hair collection and detection method of the present invention. A hair collection and detection method of the present invention is not limited to the description of the following embodiments.

[0027] Embodiment 1:

[0028] This embodiment provides a specific implementation method of a hair collection and detection method. Figure 1 As shown, the following steps are included:

[0029] S1 Hair sample collection: Cut the hair within 3 cm in length from the back of the head of the person to be tested to obtain the hair sample to be tested;

[0030] S2 Hair sample processing: Put the hair sample to be tested into a sampling tube filled with grinding liquid and grind it;

[0031] S3 Hair sample testing: Detect and convert the drug content in the hair sample through a drug trace analyzer.

[0032] Furthermore, in step S1, at least two hair samples to be tested are collected, each weighing not less than 50 mg, wrapped in aluminum foil, and respectively placed in evidence bags, which are then sealed; the sample number, extraction date and extractor information are filled in the evidence bags, and the extracted person presses fingerprints and signs to confirm the seal of the evidence bag. If the extracted person refuses to press fingerprints or sign, the extractor shall indicate this and record the entire extraction process on video.

[0033] Furthermore, step S1 also includes: preparing a hair sample extraction information table, recording the name of the person being extracted, the resident identification number of the person being extracted, the type of extracted hair, the extraction location, the extraction unit, the extraction personnel, and the extraction time.

[0034] Furthermore, in step S1, hair from different people should be extracted separately, packaged independently, and numbered uniformly, and residues on the extraction equipment during the sampling process should be cleaned in time.

[0035] Furthermore, in step S2, the grinding solution includes 2 ml of hair lysis solution prepared by 0.5 mg / ml proteinase K, 0.61% tris; 0.85% NACL; 1.0% TWEEN-20; 0.1% Proclin 300 purified water and 95% zirconium oxide grinding beads with a particle size of 2-5 mm.

[0036] Furthermore, in step S3, the hair sample detection includes the following steps:

[0037] S31 turns on the drug trace analyzer, inserts the ID card corresponding to this batch of products and reads it;

[0038] S32 tear open the aluminum foil bag, take out the test card, and place it horizontally on the laboratory table;

[0039] S33 Click the test button on the main interface of the applicable instrument, then click the scan barcode button, align the barcode on the reagent card with the scanning area on the right side of the machine, and after the scan is successful, the interface jumps to the start test interface;

[0040] S34: taking the supernatant of the mixed solution of drug extraction in the processed hair, adding the sample and timing;

[0041] S35 uses a drug trace analyzer to detect and automatically convert the drug content in the hair sample.

[0042] Furthermore, in step S34, the processed hair lysate in the sampling tube is sucked with a disposable pipette, 80uL is added to the sample well of the detection card, and the timing is started.

[0043] Furthermore, in step S35, the added test card is reacted outside the machine for 5 minutes, inserted into the immunofluorescence analyzer, the screen test is clicked, and the instrument display screen is waited for to display the test results and automatically print the test results.

[0044] Working principle: Figure 1 As shown, use medical scissors or serrated scissors to cut the hair within 3 cm in length at the back of the top of the head close to the surface of the scalp of the person being extracted; for hair longer than 3 cm, cut 3 cm from the root end;

[0045] The extracted hair samples should be divided into two parts, A and B (one of which is for backup), each weighing no less than 50 mg, wrapped in aluminum foil, and placed in evidence bags, which should be sealed. The evidence bags should be filled with information such as the sample number, extraction date, and extractor, and the person whose evidence is extracted should put his / her fingerprint and sign to confirm the seal of the evidence bag;

[0046] Take out some hair and cut it into 3-5mm pieces with scissors;

[0047] Accurately weigh 20 mg of shredded hair, pour it into the hair lysis solution sampler, and grind it with a grinder for 3 minutes;

[0048] Turn on the drug trace analyzer, insert the ID card corresponding to this batch of products and read it;

[0049] Tear open the aluminum foil bag, take out the test card, click the "Test" button on the main interface of the applicable instrument, then click the "Scan Barcode" button, align the barcode on the reagent card with the "" on the right side of the machine, and after the code is scanned successfully, the interface jumps to the start test interface, unscrew the sampler bottle cap, and add 3 drops to the sample well of the test card;

[0050] Start timing, insert the test paper into the detector in the indicated direction, and after five minutes of reaction, click the screen to test;

[0051] Wait for the instrument to display the test results on the display and print the test results automatically.

[0052] In the present invention, the fluorescent immunochromatography competition detection principle is adopted, the detection area on the nitrocellulose membrane is coated with drug synthetic antigen, and the control area is coated with goat anti-chicken polyclonal antibody; the sample pad is coated with a mixture in a certain proportion (drug monoclonal antibody and fluorescent microsphere marker to form a complex and chicken IGY and fluorescent microsphere marker to form a complex). After the hair sample lysate is dripped into the sample well of the detection card, when the object to be tested contains the antigen substance to be tested, the antigen substance of the species to be tested will form a complex with the monoclonal antibody labeled with fluorescent microspheres. Due to the capillary chromatography, the complex moves along the membrane band, so it cannot bind to the synthetic antigen coated on the nitrocellulose membrane. When there is no substance to be detected in the test object, the monoclonal antibody labeled with the fluorescent microspheres moves along the membrane band and combines with the synthetic antigen coated on the detection line (T line). The concentration of the antigen substance in the sample is negatively correlated with the fluorescence signal. The fluorescence immunoassay analyzer is used to calculate the sample concentration value based on the relative fluorescence signal processing value of the sample (T / C: T is the fluorescence signal of the detection line, and C is the fluorescence signal of the quality control line) and the calibration curve that comes with the reagent (the calibration curve has T / C as the vertical coordinate and concentration as the horizontal coordinate).

[0053] The above contents are further detailed descriptions of the present invention in combination with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. A hair collection and detection method, characterized in that: The following steps are involved: S1 Hair sample collection: Cut the hair within 3 cm in length from the back of the head of the person to be tested to obtain the hair sample to be tested; S2 Hair sample processing: Put the hair sample to be tested into a sampling tube filled with grinding liquid and grind it; S3 Hair sample testing: Detect and convert the drug content in the hair sample through a drug trace analyzer.

2. A hair collection and detection method as claimed in claim 1, characterized in that: In step S1, at least two hair samples to be tested are collected, each weighing no less than 50 mg, and wrapped in aluminum foil. The samples are placed in evidence bags and the evidence bags are sealed. The sample number, extraction date and extractor information are filled in the evidence bags, and the extracted person presses fingerprints and signs to confirm the seal of the evidence bag. If the extracted person refuses to press fingerprints or sign, the extractor shall indicate this and record the entire extraction process.

3. A hair collection and detection method as claimed in claim 1, characterized in that: The step S1 also includes: preparing a hair sample extraction information table, recording the name of the person being extracted, the resident identification number of the person being extracted, the type of hair being extracted, the extraction location, the extraction unit, the extraction personnel, and the extraction time.

4. A hair collection and detection method as claimed in claim 1, characterized in that: In step S1, hair from different people should be extracted separately, packaged independently, and numbered uniformly, and residues on the extraction equipment during the sampling process should be cleaned in time.

5. A hair collection and detection method as claimed in claim 1, characterized in that: In step S2, the grinding solution includes 2 ml of hair lysis solution prepared by 0.5 mg / ml proteinase K, 0.61% tris, 0.85% NACL, 1.0% TWEEN-20, 0.1% Proclin 300 purified water and 95% zirconium oxide grinding beads with a particle size of 2-5 mm.

6. A hair collection and detection method as claimed in claim 1, characterized in that: In step S3, the hair sample detection includes the following steps: S31 turns on the drug trace analyzer, inserts the ID card corresponding to this batch of products and reads it; S32 tear open the aluminum foil bag, take out the test card, and place it horizontally on the laboratory table; S33 Click the test button on the main interface of the applicable instrument, then click the scan barcode button, align the barcode on the reagent card with the scanning area on the right side of the machine, and after the scan is successful, the interface jumps to the start test interface; S34: taking the supernatant of the mixed solution of drug extraction in the processed hair, adding the sample and timing; S35 uses a drug trace analyzer to detect and automatically convert the drug content in the hair sample.

7. A hair collection and detection method as claimed in claim 6, characterized in that: In step S34, the treated hair lysate in the sampling tube is sucked with a disposable pipette, 80uL is added to the sample well of the detection card, and the timing is started.

8. A hair collection and detection method as claimed in claim 6, characterized in that: In step S35, the added test card is reacted outside the machine for 5 minutes, inserted into the immunofluorescence analyzer, and the screen test is clicked to wait for the instrument display to display the test results and automatically print the test results.

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