Particle detection device and sample analyzer

Abstract

A particle detection device (3) and a sample analyzer are disclosed. The particle detection device (3) includes a light source (700), a front light assembly (200), a main mirror (100), a flow chamber assembly (400), a rear light assembly (300), and a light receiving assembly. The front light assembly (200) has a front light mirror (210), the rear light assembly (300) has a rear light mirror (310), and the flow chamber assembly (400) has a detection unit (420). The mirror tubes (310) are nested and connected to the main mirror tubes (100). The central axis of the front light channel (211), the central axis of the main channel (110), the optical axis of the front optical module, the central axis of the first rear light channel (311), and the first optical axis of the rear optical module are approximately coaxial. At least the detection unit (420) is located inside the main channel (110) and along the flow direction of the sample liquid to be tested. The central axis of the main channel (110) is located between the two ends of the detection unit (420). This eliminates the need for adjustment of the flow chamber assembly in the flow direction of the sample liquid to be tested, making the assembly and debugging steps simpler.

Description

[0001] Cross-reference to related applications

[0002] This application is based on and claims priority to Chinese Patent Application No. 202221733952.0, filed on July 6, 2022, the entire contents of which are incorporated herein by reference. Technical Field

[0003] This invention relates to the field of medical devices, and in particular to a particle detection device and a sample analyzer. Background Technology

[0004] Common particle detection devices (such as cell detection devices) are based on flow cytometry technology to classify and count particles. The basic measurement principle is as follows: sample particles (such as blood cells) treated with reagents are encapsulated in sheath fluid and pushed through a flow chamber one by one under pressure. A laser emitted by a light-emitting component illuminates the sample particles in the flow chamber, causing scattering. This scattering is then processed by a light-receiving component, generating various signals such as scattered light, fluorescence, and light absorption. Based on these signals, different types of particles are classified and counted. To achieve accurate particle or cell detection, the laser excitation module, flow chamber module, and signal collection module typically require high optical alignment accuracy. Currently, most particle detection devices on the market connect these modules to a substrate via sliding mechanisms, requiring individual adjustment of each module. This multi-dimensional adjustment process results in higher production costs and lower production efficiency. Summary of the Invention

[0005] This invention aims to at least solve one of the technical problems existing in the prior art. To this end, this invention proposes a sample analyzer that can reduce debugging costs and improve debugging efficiency.

[0006] The present invention also proposes a sample analyzer that applies the aforementioned particle detection device.

[0007] The particle detection device according to the third embodiment of the present invention includes:

[0008] A light source, used to provide illumination;

[0009] A front light assembly includes a front light barrel and a front light optical module. The front light barrel includes a front light channel, and the front light optical module is disposed within the front light channel. The illumination light passes through the front light optical module, and the front light optical module shapes the illumination light.

[0010] The main lens barrel has a main channel through which the irradiation light, shaped by the front optical module, passes;

[0011] A flow chamber assembly is connected to the main microscope tube. The flow chamber assembly has an injection section, a detection section, and a discharge section arranged sequentially along the flow direction of the sample liquid to be tested. The detection section includes an incident side facing the front light assembly and an exit side opposite to the incident side. The irradiation light, shaped by the front light optical module, enters the detection section from the incident side and exits from the exit side after irradiating the sample liquid to be tested.

[0012] The rear light assembly includes a rear light lens barrel and a rear light optical module. The rear light lens barrel has at least a first rear light channel. The rear light optical module is at least partially disposed within the first rear light channel. The rear light optical module has at least a first optical axis. The irradiation light emitted from the emission side of the detection unit passes through the rear light optical module. The rear light optical module shapes the irradiation light emitted from the emission side of the detection unit.

[0013] A light receiving component receives the irradiated light that has been shaped by the post-light component;

[0014] The front light tube is nested and connected to the main light tube, and the rear light tube is nested and connected to the main light tube. The central axis of the front light channel, the central axis of the main channel, the optical axis of the front light optical module, the central axis of the first rear light channel, and the first optical axis of the rear light optical module are substantially coaxial. At least the detection unit is located in the main channel and along the flow direction of the sample liquid to be tested. The central axis of the main channel is located between the two ends of the detection unit.

[0015] The sample extraction device according to embodiments of the present invention has at least the following beneficial effects:

[0016] The detection unit is located within the main channel, ensuring that the illumination light emitted from the front light assembly can illuminate the detection unit within the main channel. Along the flow direction of the sample liquid, the central axis of the main channel is located at both ends of the detection unit. That is, after the flow chamber assembly, main lens barrel, front light assembly, and rear light assembly are assembled, the positions of the detection unit, front light assembly, and rear light assembly correspond along the flow direction of the sample liquid. This ensures that the illumination light emitted from the front light assembly can illuminate the sample liquid within the detection unit, and the illumination light emitted from the detection unit can also accurately pass through the rear light optical module. This eliminates the need for adjustment of the flow chamber assembly in the flow direction of the sample liquid, simplifying the assembly and debugging process.

[0017] In addition, the front and rear optical tubes are nested and connected to the main tube, respectively. Thus, the displacement in the radial plane between the front and main tubes and between the rear and main tubes is restricted by the mutually abutting tube walls. Therefore, precise radial positioning can be achieved simply by ensuring the machining accuracy of the tubes.

[0018] In other embodiments of the present invention, one of the main microscope tube and the flow chamber assembly is provided with a third positioning part, and the other is provided with a fourth positioning part. The flow chamber assembly is configured to move relative to the main microscope tube along a first direction via the third positioning part and the fourth positioning part. The first direction is radially parallel to the main channel, which is perpendicular to the flow direction of the sample liquid to be tested.

[0019] In other embodiments of the present invention, the injection section is provided with the third positioning section, and the main body barrel is provided with the fourth positioning section. The third positioning section includes a first positioning surface disposed on the injection section, and the fourth positioning section includes a second positioning surface disposed on the main body barrel. The first positioning surface and the second positioning surface are both perpendicular to the axial direction of the main channel and fit together to restrict the movement of the flow chamber assembly and the main body barrel along the axial direction of the main channel.

[0020] And / or, the injection section is provided with the third positioning section, and the main body microscope tube is provided with the fourth positioning section. The third positioning section includes a third positioning surface disposed on the injection section, and the fourth positioning section includes a fourth positioning surface disposed on the main body microscope tube. The third positioning surface and the fourth positioning surface are both perpendicular to the flow direction of the sample liquid to be tested, and they are in contact with each other to restrict the movement between the flow chamber assembly and the main body microscope tube along the flow direction of the sample liquid to be tested.

[0021] In other embodiments of the present invention, the injection section is connected to the bottom of the main body tube. When the main body tube has the second positioning surface and the fourth positioning surface, the particle detection device further includes a support member connected to the main body tube. The support member abuts the injection section against the fourth positioning surface along the flow direction of the sample liquid to be tested, and abuts the injection section against the second positioning surface along the axial direction of the main channel.

[0022] In other embodiments of the invention, the support member elastically abuts against the injection portion, and the flow chamber assembly is configured to move relative to the main body barrel in the first direction when the support member elastically abuts against the injection portion.

[0023] In other embodiments of the present invention, the outer peripheral surface of the main lens barrel has a mounting groove, and the liquid injection portion is at least partially located within the mounting groove, wherein:

[0024] When the main body lens barrel has the second positioning surface, the groove wall surface of the mounting groove perpendicular to the axial direction of the main body channel is set as the second positioning surface;

[0025] And / or, when the main body tube has the fourth positioning surface, the groove wall surface of the mounting groove perpendicular to the flow direction of the sample liquid to be tested is set as the fourth positioning surface.

[0026] In other embodiments of the present invention, the liquid injection part includes a first mounting part and a second mounting part. The first mounting part is perpendicular to the axial direction of the main channel and has a first positioning surface. The second mounting part is connected to the first mounting part and is perpendicular to the flow direction of the sample liquid to be tested. The second mounting part has the third positioning surface. The detection part is connected to the second mounting part.

[0027] The outer peripheral surface of the main microscope tube is provided with a mounting groove. The groove wall surface of the mounting groove perpendicular to the axial direction of the main channel is the second positioning surface, and the groove wall surface of the mounting groove perpendicular to the flow direction of the sample liquid to be tested is the fourth positioning surface.

[0028] Both the first mounting portion and the second mounting portion are at least partially located within the mounting groove.

[0029] In other embodiments of the present invention, the particle detection device further includes a threaded fastener, the first mounting portion having a connecting hole extending through to the first positioning surface, the connecting hole extending along the first direction, the second positioning surface of the main body lens barrel having a threaded hole, the threaded fastener having a first state restricting the movement of the flow chamber assembly and a second state of unlocking, the threaded fastener being inserted through the connecting hole and screwed into the threaded hole in the first state, and the threaded fastener being separated from the threaded hole in the second state, so that the flow chamber assembly can move relative to the main body lens barrel along the first direction.

[0030] In other embodiments of the present invention, the wall of the main channel is provided with a second hole extending to the outer surface of the main lens barrel. The second hole extends along the first direction. The liquid injection part passes through the second hole and is movable within the second hole along the first direction. The liquid injection part is provided with the third positioning part, and the main lens barrel is provided with the fourth positioning part. The fourth positioning part includes the hole wall of the second hole extending along the first direction, and the third positioning part includes the side of the hole wall where the liquid injection part abuts.

[0031] In other embodiments of the present invention, the injection part includes a second mounting part and a boss disposed along the flow direction of the sample liquid to be tested. Along the flow direction of the sample liquid to be tested, the end of the boss away from the second mounting part is connected to the detection part, wherein the boss passes through the second hole, and the third positioning part includes the boss abutting against the side of the hole wall.

[0032] In other embodiments of the present invention, the particle detection device further includes a locking member connected to the main body tube and the flow chamber assembly, having a first state restricting the movement of the flow chamber assembly and a second state of being unlocked. When the locking member is in the second state, the flow chamber assembly is able to move relative to the main body tube along a first direction, the first direction being radially parallel to the main channel perpendicular to the flow direction of the sample liquid to be tested.

[0033] In other embodiments of the present invention, the locking member includes a threaded fastener, one of the flow chamber assembly and the main lens barrel is provided with a connecting hole, and the other is provided with a threaded hole, the connecting hole extending along the first direction, wherein the threaded fastener, when in the first state, passes through the connecting hole and is screwed into the threaded hole, and the threaded fastener, when in the second state, is separated from the threaded hole, so that the flow chamber assembly can move relative to the main lens barrel along the first direction.

[0034] In other embodiments of the present invention, the cross-section of the main channel is circular, and the front lens barrel is at least partially located within the main channel to be nested and connected with the main lens barrel;

[0035] Alternatively, the front light channel has a circular cross-section, and the main lens barrel is located within the front light channel to be nested and connected with the front light lens barrel.

[0036] In other embodiments of the present invention, the front light tube is a single front light tube, and the main lens tube is nested and connected to the single front light tube.

[0037] In other embodiments of the present invention, the cross-section of the main channel is circular, and the rear light tube is at least partially located in the main channel to be nested and connected with the main light tube;

[0038] Alternatively, the cross-section of the first rear light channel is circular, and the main lens barrel portion is located within the first rear light channel to be nested and connected with the rear light lens barrel.

[0039] In other embodiments of the present invention, the rear light optical module includes a first condenser lens and a first aperture, and the light receiving component is used to receive the illumination light emitted from the detection unit and passing through the first condenser lens and the first aperture, wherein the light receiving component is connected to the rear light lens barrel, and the first aperture is disposed between the main lens barrel and the rear light lens barrel.

[0040] In other embodiments of the present invention, the first condenser lens is connected to the main lens barrel and is located between the flow chamber assembly and the first aperture.

[0041] Alternatively, both the first condenser lens and the light receiving component are connected to the rear light barrel, and the first condenser lens is located between the first aperture and the light receiving component.

[0042] In other embodiments of the present invention, the rear light optical module includes a first condenser lens and a first aperture, and the light receiving component is used to receive the illumination light emitted from the detection unit and passing through the first condenser lens and the first aperture, wherein the first condenser lens, the first aperture and the light receiving component are all connected to the rear light lens barrel.

[0043] In other embodiments of the present invention, the optical axis of the front optical module is parallel to the central axis of the main channel, and the distance between the optical axis of the front optical module and the central axis of the main channel is within a preset range.

[0044] In other embodiments of the present invention, the distance between the optical axis of the front optical module and the central axis of the main channel is less than or equal to 0.02 mm.

[0045] In other embodiments of the present invention, the optical axis of the front optical module intersects the central axis of the main channel, and the angle between the optical axis of the front optical module and the central axis of the main channel is within a preset range.

[0046] In other embodiments of the present invention, the angle between the optical axis of the front optical module and the central axis of the main channel is less than or equal to 5 mrad.

[0047] The particle detection device according to the fourth embodiment of the present invention includes:

[0048] A light source, used to provide illumination;

[0049] A front light assembly, including a front light optical module, wherein the front light optical module shapes the irradiated light;

[0050] The main lens barrel has a main channel through which the irradiation light, shaped by the front optical module, passes;

[0051] A flow chamber assembly is connected to the main microscope tube. The flow chamber assembly has an injection section, a detection section, and a discharge section arranged sequentially along the flow direction of the sample liquid to be tested. The detection section includes an incident side facing the front light assembly and an exit side opposite to the incident side. The irradiation light, shaped by the front light optical module, enters the detection section from the incident side and exits from the exit side after irradiating the sample liquid to be tested.

[0052] A rear light assembly includes a rear light optical module, the rear light optical module having at least a first optical axis, the irradiation light emitted from the emission side of the detection unit passes through the rear light optical module, and the rear light optical module shapes the irradiation light emitted from the emission side of the detection unit;

[0053] A light receiving component receives the irradiated light that has been shaped by the post-light component;

[0054] The central axis of the main channel, the optical axis of the front optical module, and the first optical axis of the rear optical module are approximately coaxial. At least the detection unit is located within the main channel and along the flow direction of the sample liquid to be tested. The central axis of the main channel is located between the two ends of the detection unit.

[0055] In other embodiments of the present invention, one of the main microscope tube and the flow chamber assembly is provided with a third positioning part, and the other is provided with a fourth positioning part. The flow chamber assembly is configured to move relative to the main microscope tube along a first direction via the third positioning part and the fourth positioning part. The first direction is radially parallel to the main channel, which is perpendicular to the flow direction of the sample liquid to be tested.

[0056] In other embodiments of the present invention, the injection section is provided with the third positioning section, and the main body barrel is provided with the fourth positioning section. The third positioning section includes a first positioning surface disposed on the injection section, and the fourth positioning section includes a second positioning surface disposed on the main body barrel. The first positioning surface and the second positioning surface are both perpendicular to the axial direction of the main channel and fit together to restrict the movement of the flow chamber assembly and the main body barrel along the axial direction of the main channel.

[0057] And / or, the injection section is provided with the third positioning section, and the main body microscope tube is provided with the fourth positioning section. The third positioning section includes a third positioning surface disposed on the injection section, and the fourth positioning section includes a fourth positioning surface disposed on the main body microscope tube. The third positioning surface and the fourth positioning surface are both perpendicular to the flow direction of the sample liquid to be tested, and they are in contact with each other to restrict the movement between the flow chamber assembly and the main body microscope tube along the flow direction of the sample liquid to be tested.

[0058] The sample analyzer according to the seventh embodiment of the present invention includes:

[0059] A sampling device used to acquire samples to be tested;

[0060] A sample preparation apparatus for receiving a sample to be tested acquired by the sampling device and mixing the sample to be tested with reagents to prepare a sample solution to be tested;

[0061] The aforementioned particle detection device;

[0062] A liquid supply device is used to supply sheath fluid to the flow chamber assembly so that particles in the sample liquid to be tested are entrained by the sheath fluid and pass through the detection section in a queue.

[0063] The controller is used to obtain the measurement result of the sample under test based on the feedback signal received by the optical receiving component.

[0064] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0065] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0066] Figure 1 This is a schematic diagram of a particle detection device in related technologies;

[0067] Figure 2 This is a three-dimensional schematic diagram of the particle detection device in one direction in an embodiment of the present invention;

[0068] Figure 3 for Figure 2 A three-dimensional schematic diagram of the medium particle detection device from another direction;

[0069] Figure 4 for Figure 2 A cross-sectional view of the particle detection device in a disassembled state. Figure 4 The cutting plane is set vertically;

[0070] Figure 5 for Figure 2 A cross-sectional view of the medium particle detection device in its assembled state. Figure 5 The cutting plane is set horizontally;

[0071] Figure 6 for Figure 2 A three-dimensional schematic diagram showing the connection between the main body of the microscope tube and the first aperture.

[0072] Figure 7 for Figure 2 A three-dimensional schematic diagram of the injection section;

[0073] Figure 8 for Figure 2 A three-dimensional schematic diagram of the hidden flow chamber component of the medium particle detection device;

[0074] Figure 9 for Figure 2 Schematic diagram of the optical path of a medium particle detection device;

[0075] Figure 10This is a three-dimensional schematic diagram of the sample analyzer in an embodiment of the present invention.

[0076] Figure label:

[0077] Sampling device 1, sample preparation device 2, particle detection device 3, display device 4, control device 5, first housing 6, second housing 7;

[0078] Front optical module 10, flow chamber module 20, signal collection module 30, detector module 40, substrate 50, sliding mechanism 60;

[0079] Main lens barrel 100, main channel 110, first channel section 111, second channel section 112, abutment surface 113, first hole 120, first positioning part 130, second hole 140, second positioning surface 150, threaded hole 151, fourth positioning surface 160, front light assembly 200, front light lens barrel 210, front light channel 211, limiting groove 212, first mounting cavity 213, second mounting cavity 214, second aperture 220, cylindrical lens 230, second condenser lens 240, aspherical lens 250, optical isolator 260, rear light assembly 300, rear light lens barrel 310, first rear light channel 3 11. Second rear light channel 312, first condensing lens 320, first aperture 330, second positioning part 331, reflector 340, third aperture 350, fourth aperture 360, third condensing lens 370, flow chamber assembly 400, liquid injection part 410, first positioning surface 411, third positioning surface 412, first mounting part 413, second mounting part 414, connecting hole 415, flange 416, boss 417, detection part 420, liquid drainage part 430, limiting member 500, support member 600, light source 700, first light receiving device 810, second light receiving device 820, first irradiation light L1 Second irradiation light L2 Third irradiation light L3 . Detailed Implementation

[0080] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0081] In the description of this invention, it should be understood that the orientation descriptions, such as up, down, front, back, left, right, etc., are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0082] In the description of this invention, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0083] In the description of this invention, unless otherwise explicitly defined, terms such as "set up," "install," and "connect" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this invention in conjunction with the specific content of the technical solution.

[0084] In the description of this invention, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0085] The basic principle of a particle detection device is to surround a sample liquid containing the particles to be tested with a sheath fluid, causing the particles to arrange themselves in a single row and pass sequentially through the detection area of ​​the flow chamber. Then, a laser irradiates the particles flowing through the detection area, and a light receiving device receives the scattered light and / or fluorescence generated after the irradiation. Based on the scattered light signal and / or fluorescence signal, relevant particle parameters can be further obtained. (Refer to...) Figure 1 This illustrates a typical particle detection device, comprising a front optical module 10, a flow chamber module 20, a signal collection module 30, a detector module 40, a substrate 50, and multiple sliding mechanisms 60. The sample liquid to be tested flows vertically through the flow chamber module 20. Light emitted from the front optical module 10 illuminates the detection area of ​​the flow chamber module 20. The light emitted from the flow chamber module 20 is collected by the signal collection module 30 and finally received by the detector module 40. Since the size of the particles to be tested is typically very small, the accuracy requirements for the entire optical path are very high. To ensure the accuracy of the optical path, Figure 1 The particle detection device in the process connects the front optical module 10, the flow chamber module 20, the signal collection module 30, and the detector module 40 to the substrate 50 via a sliding mechanism 60. During the assembly process, the operator adjusts the position of each module with respect to the others through the sliding mechanism 60. Since there are a large number of modules and the modules involve movement in at least two dimensions, the overall debugging process is very complicated, which significantly increases the production cost.

[0086] Based on the above problems, the present invention proposes a particle detection device, which connects at least two modules by using the main lens barrel 100 as a medium. The accuracy of the optical path is ensured by the processing accuracy of the main lens barrel 100 and the assembly accuracy of the main lens barrel 100 with other modules, thereby significantly reducing the debugging difficulty and lowering the cost. Different assembly schemes will be described below with reference to the accompanying drawings and different embodiments.

[0087] For ease of description, the terms used in this invention will first be explained:

[0088] "The flow direction of the sample solution to be tested" refers to the flow direction of the sample solution containing the particles to be tested in the flow chamber. When the particle detection device... Figure 4 When placed on a horizontal plane, the "flow direction of the sample liquid" can be understood as... Figure 4 The direction from bottom to top.

[0089] "The axial direction of the main channel" refers to the direction of extension of the central axis of the main channel. When the particle detection device... Figure 4 When the structure is placed on a horizontal plane, the "axis of the main channel" can be understood as the direction from front to back or from back to front. In some places, the "axis of the main channel" is simply expressed as the axis.

[0090] "The radial direction of the main channel perpendicular to the flow direction of the sample liquid" refers to the diameter of any cross-section of the main channel that is perpendicular to the flow direction of the sample liquid. When the particle detection device... Figure 5 When placed on a horizontal plane, the "radial direction of the main channel perpendicular to the flow direction of the sample liquid to be tested" can be understood as passing through the central axis of the main channel and flowing from left to right or from right to left.

[0091] "First direction" refers to the direction parallel to the radial direction of the main channel perpendicular to the flow direction of the sample liquid. In other words, when the first direction is involved, it is not required that it pass through the central axis of the main channel. When a particle detection device... Figure 5 When the object is placed on a horizontal plane, the "first direction" can be understood as either from left to right or from right to left.

[0092] "Approximately coaxial" refers to the situation where the central axes are completely coaxial with each other, between the central axis and the optical axis, and between the central axis and the optical axis, or within the allowable error range. Specifically, it includes two situations: one situation is that the axes are parallel to each other and the distance is within a preset range, which includes 0. That is, the axes can either coincide or deviate from each other by a certain distance; the other situation is that the axes intersect and the included angle is within a preset range.

[0093] Reference Figures 2 to 5The figures show a three-dimensional schematic diagram and a cross-sectional view of the particle detection device in an embodiment of the present invention, respectively. Figure 4 The cutting surface in the image is a vertical plane passing through the axis of the main lens barrel 100, and Figure 4 The modules within are in a decomposed state. Figure 5 The cross-section is a horizontal plane passing through the axis of the main lens barrel 100. The particle detection device mainly consists of the main lens barrel 100, the front light assembly 200, the rear light assembly 300, the flow chamber assembly 400, the light source 700, and the light receiving assembly. The light source 700 is used to provide illumination light. The flow chamber assembly 400 has a flow channel through which the sample liquid to be tested passes. The front light assembly 200 is used to shape the illumination light emitted from the light source 700 and incident on the flow chamber assembly 400. The rear light assembly 300 is used to shape the illumination light emitted from the flow chamber assembly 400. The light receiving assembly is used to receive the illumination light shaped by the rear light assembly.

[0094] The front light assembly 200 includes a front optical module, which includes an optical shaping element. Irradiation light from the light source 700 is shaped by the shaping element and then irradiates the flow chamber assembly 400, forming a light spot on the detection section 420 of the flow chamber assembly 400. The rear light assembly 300 includes a rear optical module, which includes a first condenser lens and a first aperture element. Irradiation light emitted from the flow chamber assembly 400 is received by a light receiving assembly after passing through the first condenser lens and the first aperture. The light receiving assembly includes a light receiving device for receiving optical signals and converting them into electrical signals.

[0095] The flow chamber assembly 400 includes a detection section 420 for the passage of a sample liquid to be tested. The detection section 420 is made of a light-transmitting material such as quartz glass and has a vertically extending flow channel inside, through which the sample liquid to be tested and a sheath enclosing it flow. The detection section 420 has an incident side (e.g., a front side) facing the front light assembly 200 and an exit side (e.g., a rear side) opposite to the incident side. Irradiation light shaped by the front light optical module enters the detection section from the incident side and exits from the exit side after irradiating the sample liquid to be tested. Overall, the light emitted from the light source 700 is shaped by the front light optical module, enters the detection section 420 from the incident side, irradiates the sample liquid to be tested, exits from the exit side, is shaped by the rear light assembly 300, and is then received by the light receiving assembly.

[0096] The main lens barrel 100 serves as the primary mounting structure, used to mount two or more modules. The main lens barrel 100 has a main channel 110 for light emitted through the front optical assembly 200. By nesting the main lens barrel 100 with other structures such as the front and rear optical lens barrels, the alignment of the optical path can be ensured by utilizing the precision of the inter-lens fit, thereby significantly reducing debugging difficulty and cost. In some embodiments, such as... Figures 2 to 5As shown, the main lens barrel is a cylindrical structure, which facilitates processing and further ensures processing accuracy.

[0097] Based on the above structure, the basic scheme of nesting and connecting the front-light tube and the main tube is described first, referring to... Figure 4 , Figure 5 The front light assembly 200 of the first embodiment of the present invention further includes a front light lens barrel 210, which has a front light channel 211. A front light optical module is disposed within the front light channel 211. Irradiated light passes through the front light optical module and is shaped by the front light optical module. In this embodiment, both the light source 700 and the front light optical module are connected to the front light lens barrel 210. In other embodiments, the front light optical module is connected to the front light lens barrel 210. The front light lens barrel 210 is nested with the main lens barrel 100. After the front light lens barrel 210 and the main lens barrel 100 are nested, the central axis of the front light channel 211, the central axis of the main channel 110, and the optical axis of the front light optical module are approximately coaxial.

[0098] There are two solutions for the nested connection between the front lens barrel 210 and the main lens barrel 100:

[0099] In one embodiment, the cross-section of the main channel 110 is a circle, and the front optical lens barrel 210 is at least partially located within the main channel 110. In other words, this embodiment involves the front optical lens barrel 210 being inserted into the main lens barrel 100. Figure 5 As shown in the example, the rear end of the front optical lens barrel 210 is inserted into the front end of the main body channel 110. In other embodiments, the entire front optical lens barrel 210 is inserted into the main body lens barrel 100. To accommodate the insertion fit, the outer contour of the insertion part of the front optical lens barrel 210 is set as a circle, and it is adapted to the shape and size of the main body channel 110. In the illustrated embodiment, the front optical lens barrel 210 is set as a cylindrical structure. After the two are assembled, the displacement between the front optical lens barrel 210 and the main body lens barrel 100 in the radial plane is restricted by the mutually abutting cylindrical walls. Therefore, it is only necessary to ensure the accuracy of the outer circle of the front optical lens barrel 210 and the accuracy of the inner circle of the main body lens barrel 100 to achieve the alignment of the lens barrel. Combined with the processing and assembly accuracy to ensure the precise installation between the optical components and the lens barrel, the illumination light emitted after being shaped by the front optical component 200 can accurately illuminate the particles passing through the detection section 420, which simplifies the structure and significantly reduces the debugging steps and debugging costs. It is understandable that the cross-sections of the main channel 110 and the insertion part of the front light tube 210 in this solution are circular. Compared with other non-circular cross-section cavities and insertion parts (such as polygonal cavities including rectangles, or irregular cavities with planes and arcs on the inner wall), high processing accuracy can be guaranteed through relatively simple processes. For example, a high-precision circular channel or circular outer contour can be obtained by rotating and cutting the blank with a tool.

[0100] In another embodiment, the front optical channel 211 has a circular cross-section, and the main lens barrel 100 is partially located within the front optical channel 211. In other words, this embodiment involves the main lens barrel 100 being inserted into the front optical lens barrel 210. For example, the front end of the main lens barrel 100 is inserted into the rear end of the front optical channel 211. To accommodate the insertion fit, the outer contour of the insertion portion of the main lens barrel 100 is set to a circle and adapted to the shape and size of the front optical channel 211. For example, the main lens barrel 100 is set as a cylindrical structure. Similarly, this embodiment can also simplify the structure and reduce debugging steps and costs. It should be noted that the cross-sectional shape of the main channel 110 in this embodiment is not limited to a circle.

[0101] Furthermore, in this embodiment, the front light assembly 200 includes a single front light lens barrel 210, and the main lens barrel 100 is nested and connected to the single front light lens barrel 210. At least the front light optical module is indirectly connected to the main lens barrel 100 through the front light lens barrel 210. In other words, in this embodiment, the front light part has one and only one lens barrel directly nested and connected to the main lens barrel 100, and the lens barrel directly nested and connected to the main lens barrel 100 is the front light lens barrel 210 of this embodiment. The front light optical module includes multiple lenses. In some embodiments, the multiple lenses are directly connected to the front light lens barrel 210. The position between the multiple lenses relies on the processing accuracy and assembly accuracy of the front light lens barrel 210 to ensure that the optical axis is approximately coaxial. Its structure is relatively simple and can reduce the number of components. In other embodiments, the front optical module further includes at least one secondary lens barrel. At least some of the multiple lenses are first connected to the secondary lens barrel, and then connected to the front optical lens barrel 210 through the secondary lens barrel. The multiple lenses are maintained by the processing and assembly precision of the front optical lens barrel 210 and the secondary lens barrel, thus enabling modular assembly of the front optical module. It should be noted that even when the front optical module has a secondary lens barrel, only the front optical lens barrel 210 is nested and connected to the main lens barrel 100. The secondary lens barrels are all indirectly connected to the main lens barrel 100 through the front optical lens barrel 210. In this way, the front optical assembly 200 can be pre-assembled individually, and then the front optical lens barrel 210 is nested and connected to the main lens barrel 100, which facilitates assembly.

[0102] Based on the aforementioned nested connection between the front optical lens barrel 210 and the main lens barrel 100, in some embodiments, the light source 700 and the main lens barrel 100 can move relative to each other along the axial direction of the main channel 110 to adjust the distance between the light source 700 and the flow chamber assembly 400. Specifically, when the light source 700 is connected to the front optical lens barrel 210, the front optical lens barrel 210, the light source 700, and the front optical module can move synchronously relative to the main lens barrel 100. Alternatively, the front optical lens barrel 210 can remain fixed relative to the main lens barrel 100, while the light source 700 moves independently relative to the front optical lens barrel 210 (main lens barrel 100). For example, the light source 700 can be slidably connected to the front optical lens barrel 210 via a secondary lens barrel. When the light source 700 is not directly connected to the front optical lens barrel 210, the front optical lens barrel 210 remains fixed relative to the main lens barrel 100, while the light source 700 moves independently relative to the front optical lens barrel 210 (main lens barrel 100). Figure 5 As shown in the example, the light source 700 is connected to the front light lens barrel 210, and can apply a certain external force to the front light lens barrel 210 to overcome the friction between the two, thereby driving the front light lens barrel 210 to slide within the main lens barrel 100. In other embodiments, an axial screw or other structure can also be provided between the front light lens barrel 210 and the main lens barrel 100, and the relative movement between the two lens barrels can be driven by the rotation of the screw.

[0103] When the front optical lens barrel 210 and the main lens barrel 100 can move relative to each other, some embodiments of the particle detection device also include a limiting member 500. The limiting member 500 is connected to one of the front optical lens barrel 210 and the main lens barrel 100, and can act on the other to maintain their relative positions along the axial direction after movement, thus preventing relative movement between the front optical lens barrel 210 and the main lens barrel 100 after adjustment. Figure 4 As shown, the limiting member 500 can be an elastic pin, comprising a cylindrical body with an elastic element inside and a ball bearing at the end of the body, the elastic element abutting against the ball bearing. The cylindrical wall of the main lens barrel 100 has radial mounting holes, and the limiting member 500 is located within these holes. The roller abuts against the outer wall of the front optical lens barrel 210. Thus, during the adjustment process, the operator can easily move the front optical lens barrel 210, and after movement, the limiting member 500 increases the friction between the front optical lens barrel 210 and the main lens barrel 100, preventing them from moving arbitrarily. In other embodiments, the limiting member 500 can be a magnet, and the front optical lens barrel 210 is made of a ferromagnetic material, maintaining the axial relative position between the lens barrels through magnetic adsorption.

[0104] It is understandable that when the main lens barrel 100 is inserted into the front optical lens barrel 210, the limiting member 500 is fixed to the front optical lens barrel 210 and abuts against the outer wall of the main lens barrel 100.

[0105] It should be noted that the limiting member 500 in this embodiment is used for the initial fixation of the lens barrel during the assembly process. After the debugging is completed, the main lens barrel 100 and the front optical lens barrel 210 can be fixed by adhesive or threaded fasteners.

[0106] When the front optical lens barrel 210 and the main lens barrel 100 are movable relative to each other, the particle detection device in some embodiments further includes a limiting member 500. The limiting member 500 is connected to one of the front optical lens barrel 210 and the main lens barrel 100, and can act on the other to restrict the relative rotation between the front optical lens barrel 210 and the main lens barrel 100 in the circumferential direction. For example, if the front optical lens barrel 210 is inserted into the main lens barrel 100, the limiting member 500 is the aforementioned pin or screw, and is fixed to the main lens barrel 100. The outer wall surface of the front optical lens barrel 210 is provided with a flat surface, and the pin or screw abuts against the flat surface. Alternatively, the outer wall surface of the front optical lens barrel 210 is provided with a groove, and the pin or screw is inserted into the groove.

[0107] Specifically, in some embodiments, one of the front optical lens barrel 210 and the main lens barrel 100 has a limiting groove 212, and the limiting member 500 is inserted into the limiting groove 212 to restrict circumferential rotation. Simultaneously, along the radial direction pointing towards the central axis of the main channel 110, the distance between the two side walls of the limiting groove 212 gradually decreases, and the limiting member 500 abuts against the two side walls of the limiting groove 212, thus achieving alignment between the front optical lens barrel 210 and the main lens barrel 100. See details... Figure 4 The limiting groove 212 is provided on the outer wall of the front optical lens barrel 210 and extends along the axial direction of the lens barrel. The cross-section of the limiting groove 212 is set as V-shaped for easy processing.

[0108] Based on the aforementioned nested connection between the front optical lens barrel 210 and the main optical lens barrel 100, the main optical lens barrel 100 can also be used to connect at least some of the components in the rear optical assembly 300, as shown in the figure. Figure 4 , Figure 5 In some embodiments, the rear light assembly 300 includes a rear light optical module, which includes a first condenser lens 320 and a first aperture 330. The first condenser lens 320 is used to focus the light, thereby reducing the light spot illuminating the light receiving device, which in turn reduces the target area of ​​the light receiving device, ultimately reducing the size and cost of the light receiving device. At least one of the incident surface and the exit surface of the first condenser lens 320 is aspherical. In this embodiment, the first condenser lens 320 is an aspherical lens, which is beneficial for better light focusing. The first aperture 330 is used to block stray light illuminating the light receiving device, thereby improving the quality of the optical signal collected by the light receiving device and improving the optical signal-to-noise ratio. The light receiving device can be a photodetector for receiving the illumination light emitted from the flow chamber assembly 400 and passing through the first condenser lens 320 and the first aperture 330.

[0109] In this embodiment, the main lens barrel 100 is also used to mount the first aperture stop 330. Specifically, the particle detection device also includes a clamping member connected to the main lens barrel 100, thereby clamping the first aperture stop 330 between the main lens barrel 100 and the clamping member, further increasing the integrated mounting function of the main lens barrel 100. In addition, compared with the method of fixing by adhesives or threaded fasteners, the clamping method is easier to assemble and disassemble. It should be noted that the clamping member can be the rear optical lens barrel of the rear optical assembly 300, or it can be other separately designed structures.

[0110] With the first aperture clamped between the main lens barrel 100 and the clamping member, refer to Figure 5 In some embodiments, the first condensing lens 320 is connected to the main lens barrel 100 and located between the flow chamber assembly 400 and the first aperture 330. Thus, the illumination light emitted from the flow chamber assembly 400 is first focused by the first condensing lens 320 before passing through the first aperture 330, reducing the area of ​​the opening on the first aperture 330. Furthermore, using the main lens barrel 100 as the mounting base for the first condensing lens 320 of the optical module allows both the first condensing lens 320 and the front optical assembly 200 to use the main lens barrel 100 as a reference, facilitating optical path alignment. The machining accuracy of the main lens barrel 100 also ensures assembly accuracy, further reducing debugging steps.

[0111] With the first aperture stop clamped between the main lens barrel 100 and the clamping member, in some embodiments the clamping member is inserted into the main channel 110 to clamp the first aperture stop 330, see reference. Figure 4 , Figure 5 The main channel 110 includes a first channel segment 111 and a second channel segment 112, which are arranged sequentially along the axial direction of the main channel 110. Specifically, the first channel segment 111 is located to the left of the second channel segment 112 in the figure. The diameter of the first channel segment 111 is smaller than that of the second channel segment 112, forming an abutment surface 113 at their junction. The front end of the clamping member is inserted into the second channel segment 112 to press the first aperture stop 330 against the abutment surface 113. Thus, the clamping member and the main lens barrel 100 are connected by an insertion method, simplifying the assembly structure between the clamping member and the main lens barrel 100 and facilitating assembly.

[0112] Specifically, in the illustrated embodiment, the first channel segment 111 can also be used to assemble the first condenser lens 320. It should be noted that, in addition to the first channel segment 111 and the second channel segment 112, the main channel 110 can also be provided with other channel segments, such as a third channel segment provided to the left of the first channel segment 111 for the insertion of the front light barrel 210.

[0113] In some specific embodiments, reference is made to Figure 2 , Figure 3 and Figure 5 The second channel segment 112 has a first hole 120 extending to the outer surface of the main lens barrel 100. The first hole 120 allows the first aperture 330 to move relative to the main lens barrel 100 along the direction of the first hole 120, which is the aforementioned first direction. Specifically, the first hole 120 is provided on one side or opposite sides of the wall of the second channel segment 112, and the first aperture 330 moves in a left-right direction. This movement allows the first aperture 330 to enter and exit the main lens barrel 100, thus facilitating its assembly. It also allows for fine-tuning of the first aperture 330's position between the main lens barrel 100 and the clamping member, thereby adjusting the position between the first aperture 330 and the first condenser lens 320. It should be noted that since the first aperture 330 can move laterally relative to the main lens barrel 100, when assembling or adjusting the first aperture 330, the clamping part does not need to be completely removed from the main channel 110. It only needs to be moved a short distance backward relative to the main lens barrel 100, which is convenient for operation.

[0114] With the first aperture clamped between the main lens barrel 100 and the clamping member, in some embodiments the clamping member is detachably connected to the main lens barrel 100. When the clamping member is in a non-clamping state, the first aperture 330 is configured to move relative to the main lens barrel 100 along the aforementioned first direction, thereby realizing the installation or adjustment of the first aperture 330. In this embodiment, the clamping member can be detachably connected to the main lens barrel 100 by the aforementioned plug-in engagement method, or it can be detachably connected to the main lens barrel 100 by threaded fasteners or the like.

[0115] It should be noted that the "non-pressurized state" referred to here includes both the state in which the pressing member is completely separated from the main channel 110 and the state in which there is a gap between the pressing member and the abutment surface 113 to allow the first aperture 330 to move.

[0116] In order to achieve positioning during the movement of the first aperture 330, refer to Figure 4 and Figure 6 In some embodiments, one of the main lens barrel 100 and the first aperture stop 330 is provided with a first positioning part 130, and the other is provided with a second positioning part 331. The first aperture stop 330 can be positioned relative to the main lens barrel 100 along a first direction (e.g., through the first positioning part 130 and the second positioning part 331). Figure 6The first positioning part 130 includes a positioning protrusion extending in a direction parallel to the axial direction of the main body channel 110, such as a positioning post disposed on the abutment surface 113. The second positioning part 331 includes a positioning recess extending in a first direction, such as a strip-shaped positioning groove disposed on the first aperture 330. The positioning protrusion is inserted into the positioning recess and can slide along the extending direction of the positioning recess for positioning. It is understood that the main lens barrel 100 may also be provided with a positioning groove and the first aperture 330 may be provided with a positioning post. Based on the aforementioned structure, the first aperture 330 in this embodiment only needs to move in the left and right direction, further reducing the adjustment process.

[0117] It should be noted that when the abutment surface 113 is provided with a positioning post, a vertical reference plane parallel to the axis of the main channel 110 is established. The projection of the first hole 120 in the reference plane should cover and be wider than the projection of the positioning post in the reference plane. In this way, the first aperture 330 can first be offset from the positioning post along the axis and enter the main channel 110 along the first direction, and then move along the axis toward the abutment surface 113 so that the positioning post is inserted into the positioning groove.

[0118] In other embodiments, one of the clamping member and the first aperture 330 may be provided with a first positioning part 130, and the other may be provided with a second positioning part 331. The first aperture 330 can move relative to the main body lens barrel 100 in a first direction through the first positioning part 130 and the second positioning part 331.

[0119] Based on the aforementioned nested connection between the front optical lens barrel 210 and the main optical lens barrel 100, the optical components of the rear optical assembly 300 can also be installed in other locations. For example, in some embodiments, the rear optical assembly 300 includes a rear optical module, which includes the aforementioned first condenser lens 320 and first aperture 330. The first condenser lens 320, the first aperture 330, and the light receiving component are all connected to the main optical lens barrel 100. In this way, the first condenser lens 320, the first aperture 330, the light receiving component, and the front optical assembly 200 all use the main optical lens barrel 100 as a reference, which facilitates the alignment of the optical path and ensures the assembly accuracy through the processing accuracy of the main optical lens barrel 100, thereby further reducing the debugging steps.

[0120] In some specific embodiments, the wall of the second channel segment 112 is provided with a first hole 120 extending to the outer surface of the main lens barrel 100. The first hole 120 is used to allow the first aperture 330 to move relative to the main lens barrel 100 along the through direction, which is the aforementioned first direction. Specifically, the first hole 120 is provided on one side or opposite sides of the wall of the second channel segment 112, and the movement direction of the first aperture 330 is left-right. Through movement in the aforementioned direction, on the one hand, the first aperture 330 can enter and exit the main lens barrel 100, thereby achieving the assembly of the first aperture 330; on the other hand, after the first aperture 330 is located between the main lens barrel 100 and the clamping member, its position can be finely adjusted, thereby adjusting the position between the first aperture 330 and the first condenser lens 320. Since the first aperture 330 can move laterally from the main lens barrel 100 without needing to enter or exit the main lens barrel 100 from the rear end, there is no need to remove or move the optical components behind the first aperture 330 when assembling or adjusting the first aperture 330.

[0121] Based on the aforementioned structure, to fix the first aperture 330, at least one end of the first aperture 330 extends from the first hole 120. The particle detection device also includes a fixing member that acts on the extended portion of the first aperture 330 to fix the first aperture 330 to the main lens barrel 100. For example, the fixing member can be a threaded fastener. The first aperture 330 is provided with a through hole, and the outer side of the main lens barrel 100 is provided with an axial threaded hole. The threaded fastener passes through the through hole and is screwed into the threaded hole to fix the first aperture 330 to the main lens barrel 100. It is understood that the main lens barrel 100 has first holes 120 on both opposite sides of its walls, and the two ends of the first aperture 330 extend from the corresponding first holes 120 and are fixed by fixing members respectively, thereby achieving a stable connection of the first aperture 330.

[0122] The foregoing embodiments were all described based on the nested connection between the front-light lens barrel 210 and the main lens barrel 100. The following description will also be based on the nested connection between the rear-light lens barrel 310 and the main lens barrel 100. (Refer to...) Figure 4 , Figure 5 The particle detection device in the second embodiment of the present invention includes a main lens barrel 100, a front light assembly 200, a rear light assembly 300, a flow chamber assembly 400, a light source 700, and a light receiving assembly. The flow chamber assembly 400, the light source 700, and the light receiving assembly can be understood with reference to the first embodiment. The front light assembly 200 in this embodiment can also be understood with reference to the first embodiment. Alternatively, the front light lens barrel 210 may not be provided, and the front light optical module may be directly connected to the main lens barrel 100, or it may be connected to the main lens barrel 100 through other components different from the front light lens barrel 210.

[0123] The rear light assembly 300 includes a rear light lens barrel 310 and a rear light optical module. The rear light lens barrel 310 has at least a first rear light channel 311, and the rear light optical module is at least partially disposed within the first rear light channel 311. Irradiation light emitted from the emission side of the detection unit 420 passes through the rear light optical module, which shapes the irradiation light emitted from the emission side of the detection unit 420. The rear light lens barrel 310 is nested with the main lens barrel 100. When the rear light lens barrel 310 and the main lens barrel 100 are nested together, the central axis of the first rear light channel 311, the central axis of the main channel 110, and the optical axis of the front light optical module are approximately coaxial.

[0124] There are two solutions for the nested connection between the rear optical lens barrel 310 and the main lens barrel 100:

[0125] In one embodiment, the cross-section of the main channel 110 is a circle, and the rear optical lens barrel 310 is at least partially located within the main channel 110. In other words, this embodiment involves the rear optical lens barrel 310 being inserted into the main lens barrel 100. Figure 5 As shown in the example, the front end of the rear optical lens barrel 310 is inserted into the rear end of the main body channel 110. In other embodiments, the entire rear optical lens barrel 310 is inserted into the main body lens barrel 100. To accommodate the insertion fit, the outer contour of the insertion part of the rear optical lens barrel 310 is set as circular and adapted to the shape and size of the main body channel 110. In the illustrated embodiment, the front end of the rear optical lens barrel 310 is set as a cylindrical structure. After the two are assembled, the displacement between the rear optical lens barrel 310 and the main body lens barrel 100 in the radial plane is restricted by the mutually abutting cylindrical walls. Therefore, it is only necessary to ensure the accuracy of the outer circle of the rear optical lens barrel 310 and the accuracy of the inner circle of the main body lens barrel 100 to achieve the alignment of the lens barrel. Combined with the processing and assembly accuracy to ensure the precise installation between the optical components and the lens barrel, the structure can be simplified and the debugging steps and debugging costs can be significantly reduced. It is understandable that the cross-sections of the main channel 110 and the insertion part of the rear light tube 310 in this solution are circular. Compared with other non-circular cross-section cavities and insertion parts (such as polygonal cavities including rectangles, or irregular cavities with planes and arcs on the inner wall), it is possible to ensure high processing accuracy through relatively simple processes. For example, a high-precision circular channel or circular outer contour can be obtained by rotating and cutting the blank with a tool.

[0126] In another embodiment, the cross-section of the first rear optical channel 311 is circular, and the main lens barrel 100 is partially located within the first rear optical channel 311. In other words, this embodiment involves the main lens barrel 100 being inserted into the rear optical lens barrel 310. For example, the rear end of the main lens barrel 100 is inserted into the front end of the first rear optical channel 311. To accommodate the insertion fit, the outer contour of the insertion portion of the main lens barrel 100 is set to be circular and adapted to the shape and size of the first rear optical channel 311. For example, the main lens barrel 100 is set as a cylindrical structure. Similarly, this embodiment can also simplify the structure and reduce debugging steps and costs. It should be noted that the cross-sectional shape of the main channel 110 in this embodiment is not limited to being circular.

[0127] It should be noted that the rear light assembly 300 typically also includes a single rear light lens barrel 310. The main lens barrel 100 is nested and connected with the single rear light lens barrel 310. In this way, at least some components of the rear light optical module can be pre-assembled with the rear light lens barrel 310 separately, and then the rear light lens barrel 310 is nested and connected with the main lens barrel 100, which facilitates assembly.

[0128] Based on the aforementioned nested connection between the rear lens barrel 310 and the main lens barrel 100, referring to Figure 4 , Figure 5 In some embodiments, the post-light optical module includes a first condenser lens 320 and a first aperture 330. The first condenser lens 320 is used to focus the light, thereby reducing the light spot illuminating the light receiving device, which in turn reduces the target area of ​​the light receiving device, ultimately reducing the size and cost of the light receiving device. At least one of the incident and exit surfaces of the first condenser lens 320 is aspherical. In this embodiment, the first condenser lens 320 is an aspherical lens, which is beneficial for better light focusing. The first aperture 330 is used to block stray light illuminating the light receiving device, thereby improving the quality of the optical signal collected by the light receiving device and improving the optical signal-to-noise ratio. The light receiving device can be a photodetector used to receive the illumination light emitted from the flow chamber assembly 400 and passing through the first condenser lens 320 and the first aperture 330.

[0129] In this embodiment, the light receiving component is connected to the rear light tube 310, and the first aperture 330 is disposed between the main lens tube 100 and the rear light tube 310. It should be noted that only the position of the first aperture 330 relative to the main lens tube 100 and the rear light tube 310 is described here, and their connection relationship is not specifically limited. The first aperture 330 can be fixed to the main lens tube 100, fixed to the rear light tube 310, or fixed by means of clamping between the main lens tube 100 and the rear light tube 310.

[0130] In some specific embodiments, the first aperture 330 is clamped between the main lens barrel 100 and the rear lens barrel 310, further increasing the integrated installation function of the main lens barrel 100. In addition, compared with the method of fixing by adhesives and threaded fasteners, the clamping method is easier to disassemble and assemble.

[0131] With the first aperture 330 clamped between the main lens barrel 100 and the rear aperture barrel 310, in some embodiments the rear aperture barrel 310 is inserted into the main channel 110 to press the first aperture 330 against it. (Refer to...) Figure 4 , Figure 5 The main channel 110 includes a first channel segment 111 and a second channel segment 112, which are arranged sequentially along the axial direction of the main channel 110. Specifically, the first channel segment 111 is located to the left of the second channel segment 112 in the figure. The diameter of the first channel segment 111 is smaller than that of the second channel segment 112, forming an abutment surface 113 at their junction. The front end of the rear optical lens barrel 310 is inserted into the second channel segment 112 to press the first aperture stop 330 against the abutment surface 113. Thus, the rear optical lens barrel 310 and the main lens barrel 100 are connected by an insertion method, simplifying the assembly structure between them and facilitating assembly.

[0132] It should be noted that, in addition to the first channel segment 111 and the second channel segment 112, the main channel 110 can also be provided with other channel segments, such as a third channel segment located to the left of the first channel segment 111 for the front light tube 210 to be inserted.

[0133] In some specific embodiments, reference is made to Figure 2 , Figure 3 and Figure 5 The second channel segment 112 has a first hole 120 extending to the outer surface of the main lens barrel 100. The first hole 120 allows the first aperture 330 to move relative to the main lens barrel 100 along a penetrating direction, as described above. The first hole 120 is located on one side or opposite sides of the wall of the second channel segment 112, and the first aperture 330 moves in a left-right direction. This movement allows the first aperture 330 to enter and exit the main lens barrel 100, facilitating its assembly. It also allows for fine-tuning of the first aperture 330's position between the main lens barrel 100 and the rear lens barrel 310, thereby adjusting the position between the first aperture 330 and the first condenser lens 320. It should be noted that since the first aperture 330 can move laterally relative to the main lens barrel 100, when assembling or adjusting the first aperture 330, the rear lens barrel 310 does not need to be completely removed from the main channel 110. It only needs to be moved a short distance backward relative to the main lens barrel 100, which is convenient for operation.

[0134] With the first aperture 330 clamped between the main lens barrel 100 and the rear aperture barrel 310, in some embodiments the rear aperture barrel 310 is detachably connected to the main lens barrel 100. When the rear aperture barrel 310 is in a non-pressurized state, the first aperture 330 is configured to move relative to the main lens barrel 100 along a first direction, thereby realizing the installation or adjustment of the first aperture 330. In this embodiment, the rear aperture barrel 310 can be detachably connected to the main lens barrel 100 by the aforementioned plug-in connection method, or it can be detachably connected to the main lens barrel 100 by threaded fasteners or the like.

[0135] It should be noted that the "non-compressed state" referred to here includes both the state in which the rear light tube 310 is completely separated from the main channel 110 and the state in which there is a gap between the rear light tube 310 and the abutment surface 113 to allow the first aperture 330 to move.

[0136] In order to achieve positioning during the movement of the first aperture 330, refer to Figure 4 and Figure 6 In some embodiments, one of the main lens barrel 100 and the first aperture stop 330 is provided with a first positioning part 130, and the other is provided with a second positioning part 331. The first aperture stop 330 can be positioned relative to the main lens barrel 100 along a first direction (e.g., through the first positioning part 130 and the second positioning part 331). Figure 6 The first positioning part 130 includes a positioning protrusion extending in a direction parallel to the axial direction of the main body channel 110, such as a positioning post disposed on the abutment surface 113. The second positioning part 331 includes a positioning recess extending in a first direction, such as a strip-shaped positioning groove disposed on the first aperture 330. The positioning protrusion is inserted into the positioning recess and can slide along the extending direction of the positioning recess for positioning. It is understood that the main lens barrel 100 may also be provided with a positioning groove and the first aperture 330 may be provided with a positioning post. Based on the aforementioned structure, the first aperture 330 in this embodiment only needs to move in the left and right direction, further reducing the adjustment process.

[0137] It should be noted that when the abutment surface 113 is provided with a positioning post, a vertical reference plane parallel to the axis of the main channel 110 is established. The projection of the first hole 120 in the reference plane should cover and be wider than the projection of the positioning post in the reference plane. In this way, the first aperture 330 can first be offset from the positioning post along the axis and enter the main channel 110 along the first direction, and then move along the axis toward the abutment surface 113 so that the positioning post is inserted into the positioning groove.

[0138] In other embodiments, one of the rear aperture barrel 310 and the first aperture stop 330 may be provided with a first positioning part 130 and the other may be provided with a second positioning part 331. The first aperture stop 330 may be moved relative to the main aperture barrel 100 along a first direction through the first positioning part 130 and the second positioning part 331.

[0139] The foregoing embodiments describe a scheme in which the first aperture 330 is pressed between the rear optical lens barrel 310 and the main optical lens barrel 100. In some alternative embodiments, the first aperture 330 can also be pressed by a separate fastener. The wall of the main optical lens barrel 100 is provided with a first hole 120 extending to the outer surface of the main optical lens barrel 100. For example, the first hole 120 is provided on the wall of the second channel segment 112. At least one end of the first aperture 330 extends out of the first hole 120. The particle detection device also includes a fastener connected to the main optical lens barrel 100 and acting on the extended portion of the first aperture 330 to fix the first aperture 330 to the main optical lens barrel 100. Alternatively, the fastener is connected to the rear optical lens barrel 310 and acts on the extended portion of the first aperture 330 to fix the first aperture 330 to the rear optical lens barrel 310.

[0140] The fastener can be a threaded fastener. For example, the first aperture 330 has a through hole, and the outer side of the main lens barrel 100 has an axial threaded hole. The threaded fastener passes through the through hole and is screwed into the threaded hole to fix the first aperture 330 to the main lens barrel 100. It can be understood that the walls on both sides of the main lens barrel 100 are provided with first holes 120, and the two ends of the first aperture 330 extend from the corresponding first holes 120 and are fixed by fasteners to achieve a stable connection of the first aperture 330.

[0141] Based on the first aperture stop being positioned between the main lens barrel 100 and the rear lens barrel 310, referring to... Figure 5 In some embodiments, the first condensing lens 320 is connected to the main lens barrel 100 and located between the flow chamber assembly 400 and the first aperture 330. Thus, the illumination light emitted from the flow chamber assembly 400 is first focused by the first condensing lens 320 and then passes through the first aperture 330, reducing the area of ​​the opening on the first aperture 330. Specifically, in the illustrated embodiment, the first condensing lens 320 is mounted on the first channel segment 111.

[0142] In other embodiments, the first condenser lens 320 is connected to the rear light barrel 310 and located between the first aperture 330 and the light receiving device, thereby improving the integration of the rear light assembly 300.

[0143] The foregoing embodiments describe a scheme in which some components of the rear optical module are connected to the rear optical lens barrel 310. In some embodiments, all optical components of the rear optical module can also be connected to the rear optical lens barrel 310. Specifically, the rear optical module includes the aforementioned first condenser lens 320 and first aperture 330, both of which are connected to the rear optical lens barrel 310. In addition, the light receiving component is also connected to the rear optical lens barrel 310. Thus, the rear optical component 300 can be pre-assembled and then connected to the main lens barrel 100 to further improve the integration of the rear optical component 300.

[0144] It should be noted that the aforementioned schemes based on the nested connection of the front optical lens barrel 210 and the main optical lens barrel 100, and the schemes based on the nested connection of the rear optical lens barrel 310 and the main optical lens barrel 100, can be combined with each other. For example, when the front optical lens barrel 210 and the main optical lens barrel 100 are nested together, the rear optical assembly 300 includes the rear optical lens barrel 310 and the rear optical optical module. The rear optical optical module is at least partially connected to the rear optical lens barrel 310, and the rear optical lens barrel 310 is nested together with the main optical lens barrel 100. The specific connection scheme can be understood by referring to the second embodiment and the corresponding specific embodiments. In addition, the rear optical lens barrel 310 can also be used as a clamping member in the first embodiment to clamp the first aperture stop 330. The specific clamping scheme can also be understood by referring to the second embodiment and the corresponding specific embodiments. Similarly, when the rear optical lens barrel 310 is nested with the main optical lens barrel 100, the front optical assembly 200 may include a front optical lens barrel 210 and a front optical module. The front optical module is connected to the front optical lens barrel 210, and the front optical lens barrel 210 is nested with the main optical lens barrel 100. The specific connection scheme can be understood by referring to the first embodiment and the corresponding specific embodiments. It is understood that when both the front optical lens barrel 210 and the rear optical lens barrel 310 are nested with the main optical lens barrel 100, both the front optical assembly 200 and the rear optical assembly 300 will be installed with the main optical lens barrel 100 as the reference. As long as the processing accuracy of the main optical lens barrel 100 and the assembly accuracy with the front optical lens barrel 210 and the rear optical lens barrel 310 respectively are ensured, the alignment of the front optical lens barrel 210 and the rear optical lens barrel 310 can be guaranteed. Compared with the method of adjusting each module separately, the adjustment steps and costs can be significantly reduced, and the assembly operation can also be simplified.

[0145] The following description will be based on the connection between the flow chamber assembly 400 and the main lens barrel 100, referring to... Figures 2 to 5The particle detection device in the third embodiment includes a main lens tube 100, a front light assembly 200, a rear light assembly 300, a flow chamber assembly 400, a light source 700, and a light receiving assembly. The flow chamber assembly 400 includes an injection section 410, a detection section 420, and a drainage section 430 arranged sequentially along the flow direction of the sample liquid. The injection section 410 is used to allow the sample liquid to be tested to flow into the detection section 420. The light emitted from the front light assembly 200 irradiates the sample liquid to be tested from the side of the detection section 420. The irradiated sample liquid is discharged from the drainage section 430 to the drainage pipe.

[0146] Specifically, refer to Figure 8 The main body tube 100 has a through second hole 140 in its wall, through which the detection unit 420 can enter the main body channel 110. The second hole 140 can be configured as a strip-shaped hole extending in the first direction to accommodate the movement of the flow chamber assembly 400 in the first direction. In addition, a portion of the liquid injection unit 410 can also pass through the second hole 140.

[0147] The front light assembly 200 includes a front light barrel 210 and a front light optical module. The front light barrel 210 has a front light channel 211, and the front light optical module is disposed within the front light channel 211. Irradiated light passes through the front light optical module, which shapes the irradiated light. The shaped light is then detected by the incident side of the detection unit 420. The rear light assembly 300 includes a rear light barrel 310 and a rear light optical module. The rear light barrel 310 has at least a first rear light channel 311, and the rear light optical module is at least partially disposed within the first rear light channel 311. The rear light optical module has at least a first optical axis; in other words, in some embodiments, the rear light optical module may also have a second optical axis, as will be described in subsequent embodiments. Irradiated light emitted from the emission side of the detection unit 420 passes through the rear light optical module, which shapes the irradiated light emitted from the emission side of the detection unit 420. In this embodiment, the front optical lens barrel 210 is nested and connected to the main optical lens barrel 100, and the rear optical lens barrel 310 is nested and connected to the main optical lens barrel 100. Furthermore, the central axis of the main channel 110, the optical axis of the front optical module, the first optical axis of the rear optical module, the central axis of the front optical lens barrel 210, and the central axis of the rear optical lens barrel 310 are approximately coaxial. In some embodiments, the front optical module includes multiple lenses and other devices, and the rear optical assembly 300 includes a rear optical module, which includes a first condenser lens 320 and a first aperture stop 330, among other devices.

[0148] In this embodiment, at least the detection unit 420 of the flow chamber assembly 400 is located within the main channel 110, so that the irradiation light emitted from the front light assembly 200 can irradiate the detection unit 420 within the main channel 110, and the irradiation light emitted after irradiating the sample liquid to be tested can be shaped by the rear light optical module. Along the flow direction of the sample liquid to be tested, the central axis of the main channel 110 (which can also be said to be the optical axis of the front optical module, the first optical axis of the rear optical module, the central axis of the front optical tube 210, or the central axis of the rear optical tube 310) is located at both ends of the detection section 420. That is, after the flow chamber assembly 400, the main tube 100, the front optical assembly 200, and the rear optical assembly 300 are assembled, along the flow direction of the sample liquid to be tested, the positions of the detection section 420, the front optical assembly 200, and the rear optical assembly 300 correspond to each other, ensuring that the irradiation light emitted from the front optical assembly 200 can irradiate the sample liquid to be tested in the detection section 420, and the irradiation light emitted from the detection section 420 can also pass accurately through the rear optical module, thereby eliminating the adjustment step of the flow chamber assembly 400 in the flow direction of the sample liquid to be tested, making the assembly and debugging steps simpler.

[0149] Furthermore, in this embodiment, the main lens barrel 100 serves as a medium connecting the flow chamber assembly 400, the front light assembly 200, and the rear light assembly 300. The flow chamber assembly 400, the front light assembly 200, and the rear light assembly 300 all use the main lens barrel 100 as the mounting reference. The main lens barrel 100 can accurately position the flow chamber assembly 400, the front light assembly 200, and the rear light assembly 300, thereby reducing debugging steps and lowering debugging costs.

[0150] Based on the third embodiment, in some embodiments, one of the main microscope tube 100 and the flow chamber assembly 400 is provided with a third positioning part, and the other is provided with a fourth positioning part. The flow chamber assembly 400 can be positioned to the main microscope tube 100 by the third and fourth positioning parts. The flow chamber assembly 400 is configured to move relative to the main microscope tube 100 along a first direction, thereby achieving the centering adjustment of the light emitted from the front light assembly 200 and the sample liquid to be tested. Specifically, the flow channel in the detection unit 420 is arranged vertically, and the particles in the sample liquid to be tested pass through the flow channel in a queue, thereby being irradiated sequentially. After the height of the detection unit 420 is positioned by the third and fourth positioning parts, it is only necessary to further limit the distance between the detection unit 420 and the light along the first direction (e.g., Figure 6By adjusting the position (left-right direction), the light emitted from the front light assembly 200 can accurately illuminate the particles in the sample liquid. In some embodiments, the flow chamber assembly 400 and the main microscope tube 100 are also provided with a limiting structure in the first direction. Once the flow chamber assembly 400 is installed in the main microscope tube 100, its position in the first direction is already determined and does not require adjustment. In this embodiment, the flow chamber assembly 400 can be moved along the first direction by the third positioning part and the fourth positioning part to adjust its position. Since the diameter of the particles is extremely small, the method of this embodiment can significantly reduce the installation accuracy requirements of the flow chamber assembly 400 and the main microscope tube 100 along the first direction, thereby reducing processing costs.

[0151] Based on the positioning of the main lens barrel 100 and the flow chamber assembly 400 through the third and fourth positioning parts, refer to Figure 7 , Figure 8 In some embodiments, the injection section 410 is provided with a third positioning section, and the main body tube 100 is provided with a fourth positioning section. The third positioning section includes a third positioning surface 412 of the injection section 410, and the fourth positioning section includes a fourth positioning surface 160 of the main body tube 100. The third positioning surface 412 and the fourth positioning surface 160 are in contact, which can restrict the relative movement between the flow chamber assembly 400 and the main body tube 100 along the flow direction of the sample liquid to be tested. At the same time, the flow chamber assembly 400 and the main body tube 100 can slide along a first direction in a plane perpendicular to the flow direction of the sample liquid to be tested.

[0152] In other embodiments, the third and fourth positioning portions can also be used for axial positioning of the flow chamber assembly 400, see reference. Figure 7 , Figure 8 The third positioning part includes a first positioning surface 411 of the liquid injection part 410 perpendicular to the axial direction of the main channel 110, and the fourth positioning part includes a second positioning surface 150 of the main lens barrel 100 perpendicular to the axial direction of the main channel. The first positioning surface 411 and the second positioning surface 150 are in contact, which can restrict the relative movement between the flow chamber assembly 400 and the main lens barrel 100 along the axial direction of the main channel 110.

[0153] Based on the positioning of the main microscope tube 100 and the flow chamber assembly 400 by the third positioning part and the fourth positioning part, in some specific embodiments the third positioning part includes the aforementioned first positioning surface 411 and third positioning surface 412, and the fourth positioning part includes the aforementioned second positioning surface 150 and fourth positioning surface 160, so that the displacement of the flow chamber assembly 400 along the flow direction and axial direction of the sample liquid to be tested is restricted.

[0154] The flow chamber assembly 400 is typically placed vertically, with the sample solution flowing from bottom to top. Therefore, the injection section 410 is connected to the lower side of the main microscope tube 100. To support and limit the flow chamber assembly 400, the particle detection device also includes a support member 600 connected to the main microscope tube 100. The support member 600 abuts the injection section 410 against the fourth positioning surface 160 along the flow direction of the sample solution and against the second positioning surface 150 along the axial direction of the main channel 110. Specifically... Figure 7 , Figure 8 In the illustrated embodiment, the support member 600 is configured as a pressure plate structure, with one end connected to the lower side of the main body tube 100, such as the fourth positioning surface 160, and the other end configured as an elastic end. The elastic end abuts against the injection part 410, providing complete vertical and axial positioning in conjunction with the fourth positioning surface 160. The second mounting part 414 also has a flange 416, with the elastic end abutting against the flange 416. In this embodiment, by setting the elasticity of the support member 600, when the support member 600 elastically abuts against the injection part 410, the flow chamber assembly 400 can move relative to the main body tube 100 along the first direction. Thus, the operator does not need to support the flow chamber assembly 400 during adjustment, facilitating operation.

[0155] When the fourth positioning part includes the aforementioned second positioning surface 150 and fourth positioning surface 160, refer to Figure 7 , Figure 8 In some embodiments, the second positioning surface 150 and the fourth positioning surface 160 on the main body microscope tube 100 are formed on the outer side of the tube. For example, a mounting groove can be formed on the outer wall of the main body microscope tube 100. Different walls of the mounting groove form the second positioning surface 150 and the fourth positioning surface 160, respectively. For example, when the main body microscope tube 100 has the second positioning surface 150, the groove wall surface perpendicular to the axial direction of the main body microscope tube 100 is set as the second positioning surface. When the main body microscope tube 100 has the fourth positioning surface 160, the groove wall surface perpendicular to the flow direction of the sample liquid is set as the fourth positioning surface 160. The mounting groove can be formed by removing material, which facilitates processing.

[0156] The injection section 410 can be formed using injection molding or similar processes. Specifically, it includes a first mounting section 413 and a second mounting section 414. The first mounting section 413 is perpendicular to the axial direction of the main channel 110 (e.g., vertically positioned). One side surface of the first mounting section 413 (e.g., the rear side surface) is designated as a first positioning surface 411, and its upper surface is the aforementioned third positioning surface 412. The second mounting section 414 is connected to one side of the first mounting section 413 and is perpendicular to the flow direction of the sample liquid (e.g., horizontally positioned). One side surface of the second mounting section 414 (e.g., the upper side surface) is designated as the aforementioned third positioning surface 412. The detection section 420 is connected to the second mounting section 414.

[0157] The main lens barrel 100 of this embodiment is provided with the aforementioned mounting groove, and the different groove walls of the mounting groove are respectively set as the second positioning surface 150 and the fourth positioning surface 160. The first mounting part 413 and the second mounting part 414 are at least partially located in the mounting groove, and the first positioning surface 411 is in contact with the second positioning surface 150, and the third positioning surface 412 is in contact with the fourth positioning surface 160.

[0158] In order to fix the flow chamber assembly 400, some embodiments of the particle detection device also include a locking member connected to the main body lens barrel 100 and the flow chamber assembly 400. The locking member has a first state that restricts the movement of the flow chamber assembly 400 and a second state that unlocks it. When in the second state, the flow chamber assembly 400 can move relative to the main body lens barrel 100 in a first direction.

[0159] The locking element can be a threaded fastener. The injection section 410 is provided with a connecting hole 415, and the main body barrel 100 is provided with a threaded hole 151. When in the first state, the threaded fastener passes through the connecting hole 415 and is screwed into the threaded hole 151. When in the second state, the threaded fastener is separated from the threaded hole 151, so that the flow chamber assembly 400 can move relative to the main body barrel 100 in a first direction. Specifically... Figure 7 , Figure 8 In the embodiment shown, the first mounting part 413 is provided with the aforementioned connecting hole 415, the connecting hole 415 extends through to the first positioning surface 411 and extends along the first direction, and the second positioning surface 150 is provided with a threaded hole 151.

[0160] In some alternative embodiments, the second hole 140 can also be used for positioning in the first direction. For example, the wall of the main channel 110 is provided with a second hole 140 extending to the outer surface of the main lens barrel 100, and the second hole 140 extends along the first direction, for example, it is configured as a strip hole. The liquid injection part 410 passes through the second hole 140 and can move within the second hole 140 along the first direction. In this embodiment, the liquid injection part 410 is provided with a third positioning part, the main lens barrel 100 is provided with a fourth positioning part, and the fourth positioning part includes the hole wall of the second hole 140 extending along the first direction, while the third positioning part includes the side of the hole wall where the liquid injection part 410 abuts.

[0161] Specifically, the injection unit 410 in this embodiment includes the aforementioned second mounting part 414 and boss 417. The second mounting part 414 and boss 417 are arranged along the flow direction of the sample liquid to be tested. For example, the boss 417 is connected to the upper side of the second mounting part 414. The end of the boss 417 away from the second mounting part 414 is connected to the detection unit 420. The second mounting part 414 and boss 417 can be molded into an integral structure by processes such as injection molding.

[0162] In this embodiment, the boss 417 passes through the second hole 140, and the third positioning part includes the boss 417 abutting against the side of the hole wall of the second hole 140. The boss 417 can be a circular boss as shown in the figure, or it can be a rectangular boss.

[0163] Based on the third embodiment, in some embodiments, the optical axis of the front light component 200 is parallel to the central axis of the main channel 110, and the distance between the optical axis and the central axis is within a preset range. It should be noted that the statement that the optical axis is parallel to the central axis and the distance between them is within a preset range includes the case where the distance is 0, that is, the optical axis can coincide with the central axis or deviate from the central axis.

[0164] In some specific embodiments, the distance between the optical axis and the central axis is less than or equal to 0.02 mm.

[0165] Based on the third embodiment, in some embodiments the optical axis of the front light component 200 intersects the central axis of the main channel 110, and the angle between the optical axis and the central axis is within a preset range.

[0166] In some specific embodiments, the angle between the optical axis and the central axis is less than or equal to 5 mrad.

[0167] It should be noted that the scheme based on the connection between the flow chamber assembly 400 and the main lens barrel 100 can be combined with the aforementioned schemes based on the nested connection between the front optical lens barrel 210 and the main lens barrel 100, and the scheme based on the nested connection between the rear optical lens barrel 310 and the main lens barrel 100. For example, the front optical assembly 200 includes the front optical lens barrel 210, the front optical module is connected to the front optical lens barrel 210, and the front optical lens barrel 210 is then nested and connected to the main lens barrel 100. The specific nesting scheme can be understood with reference to the first embodiment and the corresponding specific embodiments. As another example, the rear optical assembly 300 includes the rear optical lens barrel 310, the rear optical module is at least partially connected to the rear optical lens barrel 310, and the rear optical lens barrel 310 is then nested and connected to the main lens barrel 100. The specific nesting scheme can be understood with reference to the second embodiment and the corresponding specific embodiments.

[0168] The following will combine Figures 2 to 8Describing a typical combination of the present invention, the particle detection device includes a main lens barrel 100, a front light assembly 200, a rear light assembly 300, a flow chamber assembly 400, a light source 700, and a light receiving assembly. The main lens barrel 100 has a main channel 110 with a circular cross-section. The front light assembly 200 has a front light lens barrel 210 and a front light optical module. The front light optical module includes multiple lenses. The light source 700 and the multiple lenses are all connected to the front light lens barrel 210, and the multiple lenses are located in the front light channel 211 of the front light lens barrel 210. The rear end of the front light lens barrel 210 is inserted into the front end of the main channel 110. The rear light assembly 300 includes a rear light barrel 310 and a rear light optical module. The front end of the rear light barrel 310 is inserted into the rear end of the main channel 110. The rear light optical module includes a first condenser lens 320 and a first aperture 330. The first aperture 330 is clamped between the rear light barrel 310 and the main lens barrel 100. The first condenser lens 320 is connected within the main channel 110 and located between the flow chamber assembly 400 and the first aperture 330. The light receiving assembly is connected to the rear light barrel 310. The flow chamber assembly 400 is positioned and connected to the main lens barrel 100 via a third positioning part and a fourth positioning part, and is fixed by a locking member. The detection part 420 of the flow chamber assembly 400 is located within the main channel 110.

[0169] The above structure has the following advantages:

[0170] 1. Both the front optical lens barrel 210 and the rear optical lens barrel 310 are connected to the main lens barrel 100 by nesting. During assembly, the front optical lens barrel 210 and the rear optical lens barrel 310 are simply inserted into the main lens barrel 100, making assembly convenient.

[0171] 2. The cross-section of the main channel 110, the rear end of the front optical lens barrel 210, and the front end of the rear optical lens barrel 310 are all circular, which is easy to process and can ensure high processing accuracy. After the front optical lens barrel 210 and the rear optical lens barrel 310 are inserted into the main lens barrel 100, the position between the front optical component 200 and the rear optical component 300 is guaranteed by their respective processing accuracy. This can significantly reduce the number of sliding mechanisms and adjustment dimensions, and reduce material and adjustment costs.

[0172] 3. The front light assembly 200, the rear light assembly 300 and the flow chamber assembly 400 are all mounted with the main lens barrel 100 as the reference, which helps to reduce dimensional transfer errors.

[0173] 4. The first aperture 330 is fixed by clamping. Once the rear optical tube 310 is inserted into place, the first aperture 330 can be fixed. This makes assembly convenient, and the rear optical tube 310 will not obstruct the movement of the first aperture 330 when it is not in a clamped state.

[0174] The fourth embodiment of the present invention also proposes a particle detection device. The particle detection device in the fourth embodiment includes a main lens tube 100, a front light assembly 200, a rear light assembly 300, a flow chamber assembly 400, a light source 700, and a light receiving assembly. The flow chamber assembly 400 includes an injection section 410, a detection section 420, and a drainage section 430 arranged sequentially along the flow direction of the sample liquid. The injection section 410 is used to supply the sample liquid to be tested to flow into the detection section 420. The light emitted from the front light assembly 200 irradiates the sample liquid to be tested from the side of the detection section 420. The irradiated sample liquid is discharged from the drainage section 430 to the drainage pipe.

[0175] The front light assembly 200 includes a front light optical module that shapes the irradiated light, and the shaped light is detected from the incident side by the detection unit 420. The rear light assembly 300 includes a rear light optical module, which has at least a first optical axis. Irradiated light emitted from the exit side of the detection unit 420 passes through the rear light optical module, which shapes the irradiated light emitted from the exit side of the detection unit 420. In this embodiment, the central axis of the main channel 110, the optical axis of the front light optical module, and the first optical axis of the rear light optical module are approximately coaxial. In some embodiments, the front light optical module includes multiple lenses and other devices, and the rear light assembly 300 includes a rear light optical module, which includes a first condenser lens 320 and a first aperture 330, among other devices.

[0176] The difference between this embodiment and the third embodiment is that in the third embodiment, the front optical module is connected to the main lens barrel 100 through the front optical lens barrel 210, and the rear optical module is connected to the main lens barrel 100 through the rear optical lens barrel 310. This embodiment is not limited to this, and the front optical module and the rear optical module can be connected to the main lens barrel 100 in any direct or indirect way.

[0177] Based on the fourth embodiment, in some embodiments, one of the main microscope tube 100 and the flow chamber assembly 400 is provided with a third positioning part, and the other is provided with a fourth positioning part. The flow chamber assembly 400 can be positioned to the main microscope tube 100 by the third and fourth positioning parts. The flow chamber assembly 400 is configured to move relative to the main microscope tube 100 along a first direction, thereby achieving the centering adjustment of the light emitted from the front light assembly 200 and the sample liquid to be tested. Specifically, the flow channel in the detection unit 420 is arranged vertically, and the particles in the sample liquid to be tested pass through the flow channel in a queue, thereby being irradiated sequentially. After the height of the detection unit 420 is positioned by the third and fourth positioning parts, it is only necessary to further limit the distance between the detection unit 420 and the light along the first direction (e.g., Figure 6By adjusting the position (left-right direction), the light emitted from the front light assembly 200 can accurately illuminate the particles in the sample liquid. In some embodiments, the flow chamber assembly 400 and the main microscope tube 100 are also provided with a limiting structure in the first direction. Once the flow chamber assembly 400 is installed in the main microscope tube 100, its position in the first direction is already determined and does not require adjustment. In this embodiment, the flow chamber assembly 400 can be moved along the first direction by the third positioning part and the fourth positioning part to adjust its position. Since the diameter of the particles is extremely small, the method of this embodiment can significantly reduce the installation accuracy requirements of the flow chamber assembly 400 and the main microscope tube 100 along the first direction, thereby reducing processing costs.

[0178] Based on the positioning of the main lens barrel 100 and the flow chamber assembly 400 through the third and fourth positioning parts, refer to Figure 7 , Figure 8 In some embodiments, the injection section 410 is provided with a third positioning section, and the main body tube 100 is provided with a fourth positioning section. The third positioning section includes a third positioning surface 412 of the injection section 410, and the fourth positioning section includes a fourth positioning surface 160 of the main body tube 100. The third positioning surface 412 and the fourth positioning surface 160 are in contact, which can restrict the relative movement between the flow chamber assembly 400 and the main body tube 100 along the flow direction of the sample liquid to be tested. At the same time, the flow chamber assembly 400 and the main body tube 100 can slide along a first direction in a plane perpendicular to the flow direction of the sample liquid to be tested.

[0179] In other embodiments, the third and fourth positioning portions can also be used for axial positioning of the flow chamber assembly 400, see reference. Figure 7 , Figure 8 The third positioning part includes a first positioning surface 411 of the liquid injection part 410 perpendicular to the axial direction of the main channel 110, and the fourth positioning part includes a second positioning surface 150 of the main lens barrel 100 perpendicular to the axial direction of the main channel. The first positioning surface 411 and the second positioning surface 150 are in contact, which can restrict the relative movement between the flow chamber assembly 400 and the main lens barrel 100 along the axial direction of the main channel 110.

[0180] The sample liquid to be tested passes through the flow chamber one by one under the action of fluid dynamics. Under the illumination of the laser, a scattering signal is formed, and scattered light is emitted into the entire space. The photodetector receives the scattered light and obtains information about the sample liquid to be tested. Different collection angles of scattered light correspond to different information about the sample liquid to be tested. In related technologies, a single detector is generally used to collect scattered light from three angle ranges: small-angle forward light, medium-angle forward light, and large-angle forward light. However, the scattered light is not converged and is directly constrained by an angle aperture and received by the detector. This leads to the following shortcomings in the specific application of blood sample analyzers: the quality of the optical signal collected by the detector is poor, and a large target area detector is required to receive the scattered light. Therefore, the detectors provided by related technologies generally have the problems of poor detection signal, large size, and high cost. To solve the aforementioned problems, based on the first to fourth embodiments, some embodiments of the present invention also propose a rear light component 300, referring to... Figure 3 , Figure 5 , Figure 9 The rear optical module includes a first condenser lens 320, a first aperture 330, a reflector 340, a first light receiver 810, and a second light receiver 820. The first condenser lens 320, the first aperture 330, and the reflector 340 are arranged sequentially along the light emission direction of the front optical assembly 200, and the first light receiver 810 and the second light receiver 820 are arranged on the rear side of the reflector 340 along the light emission direction.

[0181] The first focusing lens 320 is used to converge at least the first illumination light L1 generated by the front light assembly 200 illuminating the sample liquid under test and within a first angular range, and the second illumination light L2 generated by the front light assembly 200 illuminating the sample liquid under test and within a second angular range, such as small-angle forward rays and large-angle forward rays. Specifically, the first illumination light L1 within the first angular range refers to the scattered light generated by the front light assembly 200 illuminating the sample liquid under test, whose angle with the optical axis of the light emitted by the front light assembly 200 is within a first angular range. Similarly, the second illumination light L2 within the second angular range refers to the scattered light generated by the front light assembly 200 illuminating the sample liquid under test, whose angle with the optical axis of the light emitted by the front light assembly 200 is within a second angular range.

[0182] The reflector 340 is used to reflect the light formed by the second illumination light L2 after being focused by the first condensing lens, thereby achieving the separation of the first illumination light L1 and the second illumination light L2. The first light receiving device 810 is at least used to receive the light focused by the first illumination light L1 after being focused by the first condensing lens 320, and the second light receiving device 820 is used to receive the light formed by the second illumination light L2 after being focused by the first condensing lens 320 and reflected by the reflector 340. Specifically, the light of the first illumination light L1 after being focused by the first condensing lens 320 can pass through the side of the reflector 340, or a light-transmitting part can be provided on the reflector 340, and the light of the first illumination light L1 after being focused by the first condensing lens 320 can pass through the light-transmitting part of the reflector 340. In this embodiment, the second illumination light L2 is separated by the reflector 340, and the first light receiving device 810 and the second light receiving device 820 respectively receive light with different scattering angles, which is beneficial to improving the quality of the light signal received by each light receiving device, and also beneficial to reducing the target area, volume and cost of each light receiving device.

[0183] In some embodiments, the first focusing lens 320 is further used to converge the third illumination light L3 generated by the front light assembly 200 illuminating the sample liquid to be tested and within the third angle range. The third illumination light L3 within the third angle range specifically refers to the scattered light generated by the front light assembly 200 illuminating the sample liquid to be tested and whose angle with the optical axis of the light emitted by the front light assembly 200 is within the third angle range.

[0184] Correspondingly, the first light receiving device 810 is also used to receive the light formed by the third illumination light L3 being converged by the first condensing lens 320, thereby realizing the separation of the first illumination light L1 relative to the second illumination light L2 and the third illumination light L3. The light of the third illumination light L3 after being converged by the first condensing lens 320 can pass through the side of the reflector 340, or a light-transmitting part can be provided on the reflector 340, and the light of the third illumination light L3 after being converged by the first condensing lens 320 can pass through the light-transmitting part of the reflector 340.

[0185] In some embodiments, each angle in the second angle range is smaller than each angle in the first angle range, and each angle in the third angle range is larger than each angle in the second angle range and smaller than each angle in the first angle range. Specifically, the first illumination light L1 is a large-angle scattered light, the second illumination light L2 is a small-angle scattered light, and the third illumination light L3 is a medium-angle scattered light.

[0186] In some specific embodiments, all angles in the first angle range are greater than 20°, all angles in the third angle range are greater than or equal to 10°, and all angles in the second angle range are greater than 0°. Further, all angles in the first angle range are greater than 20° and less than or equal to 45°, all angles in the third angle range are greater than or equal to 10° and less than or equal to 20°, and all angles in the second angle range are greater than 0° and less than 10°. Even further, all angles in the first angle range are greater than 20° and less than or equal to 70°, all angles in the third angle range are greater than or equal to 10° and less than or equal to 20°, and all angles in the second angle range are greater than 0° and less than 10°. Since the angles in the second angle range are relatively close to those in the third angle range, separating the second illumination light L2 and receiving it through a separate light receiving device can reduce errors and improve recognition accuracy.

[0187] Collecting scattered light from different angles can reflect different information about the particles being tested. Specifically, collecting the second illumination light L2 at 0°~10° is mainly used to reflect the particle size. Since the third illumination light L3 at 10°~20° is more sensitive to the refractive index of the cell membrane, cytoplasm, and nuclear membrane, collecting the third illumination light L3 at 10°~20° is mainly used to reflect the particle size (complexity) information. Collecting the first illumination light L1 at 20°~70° is mainly used to increase the accuracy of particle identification. In one specific embodiment, the light receiving device is used to detect white blood cell differential count values. In this embodiment, if only the second illumination light L2 and the third illumination light L3 are collected, it is difficult to distinguish eosinophils from neutrophils when testing samples with a large number of eosinophils. However, if the first illumination light L1, the second illumination light L2, and the third illumination light L3 are collected simultaneously, the accuracy of cell identification can be increased by using the scattered light from the three angle ranges collected, thus obtaining three-dimensional data of the particles. By collecting scattered light over a wide range of angles, more particle information can be obtained, thereby improving the accuracy of particle classification.

[0188] In some embodiments, both the first light receiving device 810 and the second light receiving device 820 have a light receiving area, and the light receiving areas of the first light receiving device 810 and the second light receiving device 820 are oriented in the same direction and are both oriented towards the first condenser lens 320. Along the axial direction of the main channel 110, the distance between the first light receiving device 810 and the first condensing lens 320 is smaller than the distance between the second light receiving device 820 and the first condensing lens 320. In other words, the first light receiving device 810 is closer to the first condensing lens 320 than the second light receiving device 820. The reason for this arrangement is that, due to the converging effect of the first condensing lens 320, the first illumination light L1 and the third illumination light L3 will eventually converge into a single light spot. By placing the first light receiving device 810 closer to the first condensing lens 320, the first illumination light L1 and the third illumination light L3 will illuminate the first light receiving device 810 before they converge together, thus forming two separate light spots. This makes it easier for the first light receiving device 810 to receive the light from the first illumination light L1 and the third illumination light L3 after they have been converged by the first condensing lens 320. In this embodiment, corresponding to the first illumination light L1 and the third illumination light L3, the first light receiving device 810 can be provided with two parallel light receiving areas, that is, light from two angle ranges can be received by one light receiving device, which can reduce the number of light receiving devices and help reduce costs.

[0189] In some embodiments, refer to Figure 9 The rear optical module also includes a third aperture 350, which is located between the reflector 340 and the second light receiver 820 along a direction parallel to the axis of the first rear optical channel 311. The direction parallel to the axis of the first rear optical channel 311 means that the third aperture 350 is not directly aligned with the reflector 340. The third aperture 350 is used to block stray light illuminating the second light receiver 820, thereby further improving the quality of the optical signal collected by the second light receiver 820 and improving the optical signal-to-noise ratio.

[0190] In some embodiments, refer to Figure 9 The rear optical module also includes a fourth aperture 360, which is located within the first rear optical channel 311 and is positioned between the reflector 340 and the first light receiving device 810 along the axial direction of the first rear optical channel 311. The fourth aperture 360 ​​is used to block stray light illuminating the first light receiving device 810, thereby further improving the quality of the optical signal collected by the first light receiving device 810 and improving the optical signal-to-noise ratio.

[0191] In some embodiments, refer to Figure 9The rear optical module also includes a third focusing lens 370, which is located within the first rear optical channel 311 and along the axial direction of the first rear optical channel 311 between the reflector 340 and the first light receiving device 810. The third focusing lens 370 can further converge the light irradiated to the first light receiving device 810, reduce the light spot irradiated on the first light receiving device 810, thereby reducing the target area of ​​the first light receiving device 810, and thus reducing the size and cost of the first light receiving device 810.

[0192] In some embodiments, the rear light tube 310 of this embodiment adopts a dual-channel design, and the rear light tube 310 further includes a second rear light channel 312. The first rear light channel 311 and the second rear light channel 312 are arranged in parallel. The first rear light channel 311 is at least used for the passage of light formed by the first illumination light L1 being converged by the first condenser lens 320, and the second rear light channel 312 is used for the passage of light formed by the second illumination light L2 being converged by the first condenser lens 320 and reflected by the reflector 340. Specifically, the first light receiving device 810 is disposed at the rear end of the first rear light channel 311, and the second light receiving device 820 is disposed at the rear end of the second rear light channel 312. In some embodiments, the first rear light channel 311 is also used for the passage of light formed by the third illumination light L3 being converged by the first condenser lens 320.

[0193] Some embodiments of the present invention also propose another rear light assembly 300, which differs from the aforementioned rear light assembly 300 in that: the aforementioned rear light assembly 300 is provided with a first light receiving device 810 and a second light receiving device 820. The first light receiving device 810 receives light formed by the convergence of the first illumination light L1 through the first condensing lens 320 and light formed by the convergence of the third illumination light L3 through the first condensing lens 320. In this embodiment, the rear light assembly 300 further includes a third light receiving device. The second light receiving device 820 receives light formed by the convergence of the second illumination light through the first condensing lens 320 and reflection by the reflector 340, and the third light receiving device receives light formed by the convergence of the third illumination light through the first condensing lens 320. In other words, light at different angles is received by different light receiving devices.

[0194] Similarly, in this embodiment, each angle in the second angle range is smaller than each angle in the first angle range, and each angle in the third angle range is larger than each angle in the second angle range and smaller than each angle in the first angle range. Specifically, the first illumination light L1 is a large-angle scattered light, the second illumination light L2 is a small-angle scattered light, and the third illumination light L3 is a medium-angle scattered light.

[0195] Based on the first to fourth embodiments, some embodiments of the present invention also propose a front light assembly 200, which includes a cylindrical lens 230 and a second condenser lens 240. The light source 700, the cylindrical lens 230 and the second condenser lens 240 are arranged sequentially along the axial direction of the front light channel 211, and at least the cylindrical lens 230 and the second condenser lens 240 are located within the front light channel 211.

[0196] The light source 700 is configured as a laser, capable of generating laser light. The light source 700 can move within a vertical plane perpendicular to the central axis of the front optical lens barrel 210, making the central axis of the beam approximately coaxial with the optical axis of the lens. After the light source 700 is adjusted, it can be fixed by means such as applying adhesive. The cylindrical lens 230 converges the incident beam in the flow direction of the sample liquid, and the second condenser lens 240 converges the incident beam in both the flow direction of the sample liquid and the first direction.

[0197] In some embodiments, refer to Figure 5 , Figure 9 The front optical module also includes an aspherical lens 250, which is located within the front optical channel 211 and between the light source 700 and the cylindrical lens 230. The aspherical lens 250 is used to collimate the beam emitted from the light source 700.

[0198] In some embodiments, refer to Figure 5 , Figure 9 The front optical module also includes an optical isolator 260, which is located in the front optical channel 211 and between the aspherical lens 250 and the cylindrical lens 230. The optical isolator 260 is used to prevent the incident light beam from shining on the cylindrical lens 230 and the second condenser lens 240 to form a reflected light beam, thereby improving the signal-to-noise ratio of the incident light beam shining on the sample flow in the flow chamber.

[0199] In some embodiments, refer to Figure 5 , Figure 9 The front optical module also includes a second aperture 220, which is located within the front optical channel 211 and between the light source 700 and the aspherical lens 250.

[0200] In some embodiments, refer to Figure 5 Along the axial direction of the front optical lens barrel, the front optical channel 211 includes a plurality of first mounting cavities 213 arranged sequentially. The plurality of first mounting cavities 213 extend to the rear end face of the front optical lens barrel 210 and their inner diameters increase sequentially, so as to facilitate the sequential installation of each optical device of the aforementioned front optical optical module into the front optical lens barrel 210.

[0201] In some embodiments, refer to Figure 5The front light channel 211 also includes a second mounting cavity 214, which extends to the front end face of the front light lens barrel 210. The second mounting cavity 214 is connected to the first mounting cavity 213 through an intermediate cavity. The light source 700 is at least partially located in the second mounting cavity 214, thereby connecting the light source 700 to the front light lens barrel 210.

[0202] The fifth embodiment of the present invention also proposes a sample analyzer, referring to... Figure 10 The analyzer includes a sampling device 1, a sample preparation device 2, a particle detection device 3, a display device 4, and a control device 5. The sampling device 1 acquires the sample to be tested. The sample preparation device 2 receives the sample acquired by the sampling device 1 and mixes it with reagents to prepare a sample solution. The particle detection device 3 detects the sample solution prepared by the sample preparation device 2 to obtain routine blood parameters. The display device 4 displays information related to the routine blood parameters. For example, the display device 4 may be configured as a user interface. The control device 5 includes a processor and a storage medium storing a computer program. In addition to the main devices described above, the analyzer may also include a first housing 6 and a second housing 7. The particle detection device 3 and the control device 5 are disposed inside the second housing 7, respectively on opposite sides of the second housing 7. The sample preparation device 2 is disposed inside the first housing 6. The display device 4 is disposed on the outer surface of the first housing 6.

[0203] The particle detection device 3 of this embodiment can be the particle detection device of the first embodiment and other specific embodiments based on the first embodiment. Specifically, it includes a main lens barrel 100, a front light assembly 200, a rear light assembly 300, a flow chamber assembly 400, a light source 700 and a light receiving assembly. The main lens barrel 100 has a main channel 110. The front light assembly 200 also includes a front light lens barrel 210. The front light optical module is connected to the front light lens barrel 210. The front light lens barrel 210 is nested and connected to the main lens barrel 100. In this way, the displacement between the front light lens barrel 210 and the main lens barrel 100 in the radial plane is restricted by the mutually abutting barrel walls. Therefore, it is only necessary to ensure the accuracy of the inner and outer circles of the front light lens barrel 210 and the main lens barrel 100 to achieve precise radial positioning.

[0204] Furthermore, in this embodiment, the front light assembly 200 includes a single front light lens barrel 210, and the main lens barrel 100 is nested and connected to the single front light lens barrel 210. In other words, the front light optical module is indirectly connected to the main lens barrel 100 through the front light lens barrel 210. In this way, the front light assembly 200 can be pre-assembled individually, and then the front light lens barrel 210 is nested and connected to the main lens barrel 100, which facilitates assembly.

[0205] The sixth embodiment of the present invention also proposes a sample analyzer, referring to... Figure 10The analyzer includes a sampling device 1, a sample preparation device 2, a particle detection device 3, a display device 4, and a control device 5. The sampling device 1 acquires the sample to be tested. The sample preparation device 2 receives the sample acquired by the sampling device 1 and mixes it with reagents to prepare a sample solution. The particle detection device 3 detects the sample solution prepared by the sample preparation device 2 to obtain routine blood parameters. The display device 4 displays information related to the routine blood parameters. For example, the display device 4 may be configured as a user interface. The control device 5 includes a processor and a storage medium storing a computer program. In addition to the main devices described above, the analyzer may also include a first housing 6 and a second housing 7. The particle detection device 3 and the control device 5 are disposed inside the second housing 7, respectively on opposite sides of the second housing 7. The sample preparation device 2 is disposed inside the first housing 6. The display device 4 is disposed on the outer surface of the first housing 6.

[0206] The particle detection device 3 of this embodiment can be the particle detection device of the aforementioned second embodiment and other specific embodiments based on the second embodiment. Specifically, it includes a main lens barrel 100, a front light assembly 200, a rear light assembly 300, a flow chamber assembly 400, a light source 700 and a light receiving assembly. The main lens barrel 100 has a main channel 110. The rear light assembly 300 also includes a rear light lens barrel 310. The rear light optical module is connected to the rear light lens barrel 310. The rear light lens barrel 310 is nested and connected to the main lens barrel 100. In this way, the displacement between the rear light lens barrel 310 and the main lens barrel 100 in the radial plane is restricted by the mutually abutting barrel walls. Therefore, it is only necessary to ensure the accuracy of the inner and outer circles of the rear light lens barrel 310 and the main lens barrel 100 to achieve precise radial positioning.

[0207] The seventh embodiment of the present invention also proposes a sample analyzer, referring to... Figure 10 The analyzer includes a sampling device 1, a sample preparation device 2, a particle detection device 3, a display device 4, and a control device 5. The sampling device 1 acquires the sample to be tested. The sample preparation device 2 receives the sample acquired by the sampling device 1 and mixes it with reagents to prepare a sample solution. The particle detection device 3 detects the sample solution prepared by the sample preparation device 2 to obtain routine blood parameters. The display device 4 displays information related to the routine blood parameters. For example, the display device 4 may be configured as a user interface. The control device 5 includes a processor and a storage medium storing a computer program. In addition to the main devices described above, the analyzer may also include a first housing 6 and a second housing 7. The particle detection device 3 and the control device 5 are disposed inside the second housing 7, respectively on opposite sides of the second housing 7. The sample preparation device 2 is disposed inside the first housing 6. The display device 4 is disposed on the outer surface of the first housing 6.

[0208] The particle detection device 3 of this embodiment can be a particle detection device based on the aforementioned third embodiment and other specific embodiments based on the third embodiment. Specifically, it includes a main lens barrel 100, a front light assembly 200, a rear light assembly 300, a flow chamber assembly 400, a light source 700, and a light receiving assembly. The front light assembly 200 includes a front light lens barrel 210 and a front light optical module, with the front light optical module connected to the front light lens barrel 210. The rear light assembly 300 includes a rear light lens barrel 310 and a rear light optical module, with the rear light optical module connected to the rear light lens barrel 310.

[0209] The flow chamber assembly 400 includes an injection section 410, a detection section 420, and a drainage section 430 arranged sequentially along the flow direction of the sample liquid. The injection section 410 is used to allow the sample liquid to be tested to flow into the detection section 420. At least the detection section 420 of the flow chamber assembly 400 is located in the main channel 110. Along the flow direction of the sample liquid to be tested, the central axis of the main channel 110 is located at both ends of the detection section 420. That is, after the flow chamber assembly 400, the main lens barrel 100, the front light assembly 200, and the rear light assembly 300 are assembled, the positions of the detection section 420, the front light assembly 200, and the rear light assembly 300 are corresponding along the flow direction of the sample liquid to be tested. This ensures that the irradiation light emitted from the front light assembly 200 can irradiate the sample liquid to be tested in the detection section 420, and the irradiation light emitted from the detection section 420 can also accurately pass through the rear light optical module. This eliminates the need for adjustment of the flow chamber assembly 400 in the flow direction of the sample liquid to be tested, making the assembly and debugging steps simpler.

[0210] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments, and various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof can be combined with each other unless otherwise specified.

Claims

1. A particle detection device, characterized by, include: A light source, used to provide illumination; A front light assembly includes a front light barrel and a front light optical module. The front light barrel includes a front light channel, and the front light optical module is disposed within the front light channel. The illumination light passes through the front light optical module, and the front light optical module shapes the illumination light. The main lens barrel has a main channel through which the irradiation light, shaped by the front optical module, passes. The main lens barrel is provided with a second hole that penetrates the wall of the main lens barrel. A flow chamber assembly is connected to the main body tube. The flow chamber assembly has an injection section, a detection section, and a discharge section arranged sequentially along the flow direction of the sample liquid to be tested. The detection section can enter the main body channel through the second hole and be positioned in the main body channel. The detection section includes an incident side facing the front light assembly and an exit side opposite to the incident side. The irradiation light, shaped by the front light optical module, enters the detection section from the incident side in the main body channel and exits from the exit side after irradiating the sample liquid to be tested. The rear light assembly includes a rear light lens barrel and a rear light optical module. The rear light lens barrel has at least a first rear light channel. The rear light optical module is at least partially disposed within the first rear light channel. The rear light optical module has at least a first optical axis. The irradiation light emitted from the emission side of the detection unit passes through the rear light optical module. The rear light optical module shapes the irradiation light emitted from the emission side of the detection unit. A light receiving component receives the irradiated light that has been shaped by the post-light component; With the front light tube nested with the main light tube and the rear light tube nested with the main light tube, and the detection unit entering the main channel through the second hole and positioned inside the main light tube, the central axis of the front light channel, the central axis of the main channel, the optical axis of the front light optical module, the central axis of the first rear light channel, and the first optical axis of the rear light optical module are approximately coaxial. At least the detection unit is located inside the main channel, and along the flow direction of the sample liquid to be tested, the central axis of the main channel is located between the two ends of the detection unit. The irradiation light shaped by the front light optical module enters the detection unit from the incident side in the main channel, and the irradiation light from the exit side of the detection unit enters the rear light optical module in the first rear light channel.

2. The particle detection apparatus according to claim 1, wherein One of the main microscope tube and the flow chamber assembly is provided with a third positioning part, and the other is provided with a fourth positioning part. The flow chamber assembly is configured to move relative to the main microscope tube along a first direction through the third positioning part and the fourth positioning part. The first direction is radially parallel to the main channel, which is perpendicular to the flow direction of the sample liquid to be tested.

3. The particle detection apparatus of claim 2, wherein The injection section is provided with the third positioning section, and the main body barrel is provided with the fourth positioning section. The third positioning section includes a first positioning surface disposed on the injection section, and the fourth positioning section includes a second positioning surface disposed on the main body barrel. The first positioning surface and the second positioning surface are both perpendicular to the axial direction of the main channel and fit together to restrict the movement of the flow chamber assembly and the main body barrel along the axial direction of the main channel. And / or, the injection section is provided with the third positioning section, and the main body microscope tube is provided with the fourth positioning section. The third positioning section includes a third positioning surface disposed on the injection section, and the fourth positioning section includes a fourth positioning surface disposed on the main body microscope tube. The third positioning surface and the fourth positioning surface are both perpendicular to the flow direction of the sample liquid to be tested, and they are in contact with each other to restrict the movement between the flow chamber assembly and the main body microscope tube along the flow direction of the sample liquid to be tested.

4. The particle detection apparatus of claim 3, wherein The injection section is connected to the bottom of the main microscope tube. When the main microscope tube has the second positioning surface and the fourth positioning surface, the particle detection device further includes a support member connected to the main microscope tube. The support member abuts the injection section against the fourth positioning surface along the flow direction of the sample liquid to be tested, and abuts the injection section against the second positioning surface along the axial direction of the main channel.

5. The particle detection apparatus of claim 4, wherein The support member elastically abuts against the injection portion, and the flow chamber assembly is configured to move relative to the main body barrel in the first direction when the support member elastically abuts against the injection portion.

6. The particle detection apparatus of claim 3, wherein The outer peripheral surface of the main endoscope tube has a mounting groove, and the liquid injection section is at least partially located within the mounting groove, wherein: When the main body lens barrel has the second positioning surface, the groove wall surface of the mounting groove perpendicular to the axial direction of the main body channel is set as the second positioning surface; And / or, when the main body tube has the fourth positioning surface, the groove wall surface of the mounting groove perpendicular to the flow direction of the sample liquid to be tested is set as the fourth positioning surface.

7. The particle detection device according to claim 3, characterized in that, The liquid injection section includes a first mounting section and a second mounting section. The first mounting section is perpendicular to the axial direction of the main channel and has a first positioning surface. The second mounting section is connected to the first mounting section and is perpendicular to the flow direction of the sample liquid to be tested. The second mounting section has the third positioning surface. The detection section is connected to the second mounting section. The outer peripheral surface of the main microscope tube is provided with a mounting groove. The groove wall surface of the mounting groove perpendicular to the axial direction of the main channel is the second positioning surface, and the groove wall surface of the mounting groove perpendicular to the flow direction of the sample liquid to be tested is the fourth positioning surface. Both the first mounting portion and the second mounting portion are at least partially located within the mounting groove.

8. The particle detection device according to claim 7, characterized in that, The particle detection device further includes a threaded fastener. The first mounting portion has a connecting hole extending through to the first positioning surface. The connecting hole extends along the first direction. The second positioning surface of the main body lens barrel has a threaded hole. The threaded fastener has a first state that restricts the movement of the flow chamber assembly and a second state that unlocks it. When the threaded fastener is in the first state, it passes through the connecting hole and is screwed into the threaded hole. When the threaded fastener is in the second state, it is separated from the threaded hole so that the flow chamber assembly can move relative to the main body lens barrel along the first direction.

9. The particle detection device according to claim 2, characterized in that, The main channel has a second hole extending to the outer surface of the main lens barrel. The second hole extends along the first direction. The liquid injection part passes through the second hole and can move within the second hole along the first direction. The liquid injection part is provided with the third positioning part, and the main lens barrel is provided with the fourth positioning part. The fourth positioning part includes the hole wall of the second hole extending along the first direction, and the third positioning part includes the side of the hole wall where the liquid injection part abuts.

10. The particle detection device according to claim 9, characterized in that, The injection section includes a second mounting section and a boss arranged along the flow direction of the sample liquid to be tested. Along the flow direction of the sample liquid to be tested, the end of the boss away from the second mounting section is connected to the detection section. The boss passes through the second hole. The third positioning section includes the boss abutting against the side of the hole wall.

11. The particle detection device according to claim 1, characterized in that, The particle detection device further includes a locking member connected to the main body tube and the flow chamber assembly. The locking member has a first state that restricts the movement of the flow chamber assembly and a second state that unlocks it. When the locking member is in the second state, the flow chamber assembly can move relative to the main body tube along a first direction. The first direction is radially parallel to the main channel and perpendicular to the flow direction of the sample liquid to be tested.

12. The particle detection device according to claim 11, characterized in that, The locking element includes a threaded fastener. One of the flow chamber assembly and the main lens barrel is provided with a connection hole, and the other is provided with a threaded hole. The connection hole extends along the first direction. When the threaded fastener is in the first state, it passes through the connection hole and is screwed into the threaded hole. When the threaded fastener is in the second state, it is separated from the threaded hole, so that the flow chamber assembly can move relative to the main lens barrel along the first direction.

13. The particle detection device according to claim 1, characterized in that, The main channel has a circular cross-section, and the front lens barrel is at least partially located within the main channel to be nested and connected with the main lens barrel; Alternatively, the front light channel has a circular cross-section, and the main lens barrel is located within the front light channel to be nested and connected with the front light lens barrel.

14. The particle detection device according to claim 13, characterized in that, The front light tube is a single front light tube, and the main lens tube is nested and connected to the single front light tube.

15. The particle detection device according to claim 1, characterized in that, The main channel has a circular cross-section, and the rear light tube is at least partially located in the main channel so as to be nested and connected with the main light tube; Alternatively, the cross-section of the first rear light channel is circular, and the main lens barrel portion is located within the first rear light channel to be nested and connected with the rear light lens barrel.

16. The particle detection device according to claim 1, characterized in that, The rear light optical module includes a first condenser lens and a first aperture. The light receiving component is used to receive the illumination light emitted from the detection unit and passing through the first condenser lens and the first aperture. The light receiving component is connected to the rear light lens barrel, and the first aperture is disposed between the main lens barrel and the rear light lens barrel.

17. The particle detection device according to claim 16, characterized in that, The first condenser lens is connected to the main lens barrel and is located between the flow chamber assembly and the first aperture. Alternatively, both the first condenser lens and the light receiving component are connected to the rear light barrel, and the first condenser lens is located between the first aperture and the light receiving component.

18. The particle detection device according to claim 1, characterized in that, The rear optical module includes a first condenser lens and a first aperture. The light receiving component is used to receive the illumination light emitted from the detection unit and passing through the first condenser lens and the first aperture. The first condenser lens, the first aperture, and the light receiving component are all connected to the rear optical barrel.

19. The particle detection device according to claim 1, characterized in that, The optical axis of the front optical module is parallel to the central axis of the main channel, and the distance between the optical axis of the front optical module and the central axis of the main channel is within a preset range.

20. The particle detection device according to claim 19, characterized in that, The distance between the optical axis of the front optical module and the central axis of the main channel is less than or equal to 0.02 mm.

21. The particle detection device according to claim 1, characterized in that, The optical axis of the front optical module intersects the central axis of the main channel, and the angle between the optical axis of the front optical module and the central axis of the main channel is within a preset range.

22. The particle detection device according to claim 21, characterized in that, The angle between the optical axis of the front optical module and the central axis of the main channel is less than or equal to 5 mrad.

23. A particle detection device, characterized in that, include: A light source, used to provide illumination; A front light assembly, including a front light optical module, wherein the front light optical module shapes the irradiated light; The main lens barrel has a main channel through which the irradiation light, shaped by the front optical module, passes. The main lens barrel is provided with a second hole that penetrates the wall of the main lens barrel. A flow chamber assembly is connected to the main body tube. The flow chamber assembly has an injection section, a detection section, and a discharge section arranged sequentially along the flow direction of the sample liquid to be tested. The detection section can enter the main body channel through the second hole and be positioned in the main body channel. The detection section includes an incident side facing the front light assembly and an exit side opposite to the incident side. The irradiation light, shaped by the front light optical module, enters the detection section from the incident side in the main body channel and exits from the exit side after irradiating the sample liquid to be tested. A rear light assembly includes a rear light optical module, the rear light optical module having at least a first optical axis, the irradiation light emitted from the emission side of the detection unit passes through the rear light optical module, and the rear light optical module shapes the irradiation light emitted from the emission side of the detection unit; A light receiving component receives the irradiated light that has been shaped by the post-light component; In this configuration, both the front light assembly and the rear light assembly are connected to the main lens barrel. When the detection unit enters the main channel through the second hole and is positioned within the main lens barrel, and when both the front light assembly and the rear light assembly are connected to the main lens barrel, the central axis of the main channel, the optical axis of the front light optical module, and the first optical axis of the rear light optical module are approximately coaxial. At least the detection unit is located within the main channel, and along the flow direction of the sample liquid to be tested, the central axis of the main channel is located between the two ends of the detection unit.

24. The particle detection device according to claim 23, characterized in that, One of the main microscope tube and the flow chamber assembly is provided with a third positioning part, and the other is provided with a fourth positioning part. The flow chamber assembly is configured to move relative to the main microscope tube along a first direction through the third positioning part and the fourth positioning part. The first direction is radially parallel to the main channel, which is perpendicular to the flow direction of the sample liquid to be tested.

25. The particle detection device according to claim 24, characterized in that, The injection section is provided with the third positioning section, and the main body barrel is provided with the fourth positioning section. The third positioning section includes a first positioning surface disposed on the injection section, and the fourth positioning section includes a second positioning surface disposed on the main body barrel. The first positioning surface and the second positioning surface are both perpendicular to the axial direction of the main channel and fit together to restrict the movement of the flow chamber assembly and the main body barrel along the axial direction of the main channel. And / or, the injection section is provided with the third positioning section, and the main body microscope tube is provided with the fourth positioning section. The third positioning section includes a third positioning surface disposed on the injection section, and the fourth positioning section includes a fourth positioning surface disposed on the main body microscope tube. The third positioning surface and the fourth positioning surface are both perpendicular to the flow direction of the sample liquid to be tested, and they are in contact with each other to restrict the movement between the flow chamber assembly and the main body microscope tube along the flow direction of the sample liquid to be tested.

26. A sample analyzer, characterized in that, include: A sampling device used to acquire samples to be tested; A sample preparation apparatus for receiving a sample to be tested acquired by the sampling device and mixing the sample to be tested with reagents to prepare a sample solution to be tested; The particle detection device according to any one of claims 1 to 25; A liquid supply device is used to supply sheath fluid to the flow chamber assembly so that particles in the sample liquid to be tested are entrained by the sheath fluid and pass through the detection section in a queue. The controller is used to obtain the measurement result of the sample under test based on the feedback signal received by the optical receiving component.

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