Pollination method for improving hybridization efficiency of tea trees, nutritional agent and application

Abstract

The invention belongs to the technical field of tea tree breeding, and particularly relates to a pollination method for improving the hybridization efficiency of tea trees, a nutritional agent and application. The pollination method comprises the following steps: collecting male parent pollen; emasculating the female parent, smearing the male parent pollen on the stigma of the emasculated female parent flower to complete pollination operation to form a hybrid flower; wrapping the hybrid flowers with the isolation bags; the pollination day is marked as the 0th day, the isolation bag is taken out and a nutritional agent is applied 3-5 days after pollination, and then the isolation bag is continuously used for wrapping the hybrid flowers; the formula of the nutritional agent comprises the following components: 3g to 8g of monopotassium phosphate, 0.05 to 0.1 g of cobalamin and 1L of sterile water. The application amount is to wet female parent flower stigmas; 7-9 days after pollination, the isolation bag is thoroughly removed, and development is carried out depending on the natural environment. According to the method, the fruit setting rate is increased, the young fruit falling rate of artificial hybridization is reduced, and then the hybridization efficiency is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of tea tree breeding, and in particular relates to a pollination method and a nutrient agent for improving tea tree hybridization efficiency and uses. Background Art

[0002] Tea tree hybridization is a breeding method. Tea trees with different genetic characteristics are artificially mated with male and female cells to produce hybrid offspring, which are then selected according to breeding goals to produce new varieties. Hybrid breeding is one of the main ways of tea tree breeding. The principles of parent selection for tea trees mainly include: the male and female traits complement each other; the flowering period of the male and female parents is relatively consistent; the target traits are contained in the parents; generally, the probability of producing excellent offspring is greater if the relationship is distant.

[0003] Pollination is a key step in tea hybrid breeding. To ensure correct pollination, you need to prepare isolation paper bags, pollination brushes, marking cards, scissors, tweezers, and notebooks. Collect flower buds from the male plant 1-2 days before pollination, and collect pollen from the male buds on the morning of the pollination day for hybrid pollination. The female plant is emasculated before the flowers bloom to avoid the female plant's own pollen interfering with the hybridization effect. Usually, the male plant's pollen is applied to the emasculated female plant with a brush. After pollination is completed, mark it and let it develop under natural conditions until the offspring is harvested.

[0004] For tea trees, the rate of artificially hybridized young fruits falling off is over 60%. These fallen young fruits are not mature, and their offspring are underdeveloped and cannot be used for breeding. Therefore, the efficiency of tea tree hybridization is not high. Summary of the invention

[0005] In order to improve the hybridization efficiency and reduce the young fruit shedding rate of artificial hybridization, the present invention provides a pollination method for improving the hybridization efficiency of tea trees, wherein male pollen is smeared on the stigma of emasculated female flower, and after hybridization, a nutrient is added.

[0006] The first technical solution of the present invention is as follows:

[0007] A pollination method for improving tea tree hybridization efficiency, comprising:

[0008] Collect male parent pollen and store at 0℃~4℃ for later use;

[0009] The female plant is emasculated, and the pollen of the male plant is applied to the stigma of the emasculated female plant flower to complete the pollination operation and form a hybrid flower;

[0010] Isolation bags wrap hybrid flowers;

[0011] The day of pollination is recorded as day 0. On the 3rd to 5th day after pollination, the isolation bag is removed, the nutrient agent is applied, and then the hybrid flowers are continued to be wrapped with the isolation bag;

[0012] The formula of the nutrient is: 3g-8g potassium dihydrogen phosphate, 0.5g-1g cobalamin, 1L sterile water; the application amount is to wet the stigma of the mother plant;

[0013] On the 7th to 9th day after pollination, the isolation bag is completely removed and the plants are allowed to develop in the natural environment.

[0014] It should be noted that one day after pollination is recorded as one day, such as the 1st, 2nd, 3rd, 4th, ..., nth day after pollination, where n is a positive integer.

[0015] During the above-mentioned pollination stage, the encounter between the male pollen and the emasculated female stigma is a necessary step to achieve hybridization. The genomic information is combined and recombined at this time to produce new offspring.

[0016] After pollination, the flowers are wrapped in isolation bags to prevent other pollen from interfering with the flowers. This is because the time of flowering and pollination, as well as the combination and development of genes between pollen and stigma, all have a time period and are not completed instantaneously. Therefore, the protection of isolation bags is needed to avoid interference from other pollen and other adverse factors in the environment to ensure pollination accuracy.

[0017] In order to improve the fruit setting rate of young fruits and prevent young fruits from falling off, the present invention uses a nutrient after pollination, potassium dihydrogen phosphate supplements phosphorus and potassium elements, and potassium dihydrogen phosphate becomes weakly acidic after being dissolved in water. The weakly acidic environment helps to maintain the stability of tea hybrid flowers and reduce the rate of young fruit falling off. Cobalamin is also known as vitamin B12. Four reduced pyrrole rings are connected together to become a corrin macrocycle. The structure is relatively stable in a weakly acidic environment of pH 4.5-5. The aqueous solution of potassium dihydrogen phosphate just provides the stability environment requirements of cobalamin, which facilitates the cobalamin to exert its efficacy.

[0018] Preferably, in the above-mentioned pollination method for improving tea tree hybridization efficiency, the isolation bag is removed 72h to 96h after the nutrient agent is applied. During this period, the hybrid flowers fully absorb the substances in the nutrient agent. The flowers that have just been hybridized are weak and have poor resistance, so the young fruits will fall off highly. The present invention has the protection of the isolation bag during the initial stage of pollination. On the one hand, the hybrid flowers directly absorb the nutrient elements in the nutrient agent, and on the other hand, other pathogenic substances in the environment threaten the health of the hybrid flowers.

[0019] Preferably, in the above-mentioned pollination method for improving tea tree hybridization efficiency, 100 μL to 300 μL of nutrient solution is applied to each hybrid flower, and the nutrient solution can be injected at multiple points on the surface of the female plant stigma using a syringe or a pipette.

[0020] Preferably, 150 μL to 250 μL of nutrient agent is applied to each hybrid flower. At this application amount, the young fruit shedding rate is significantly reduced and the hybridization efficiency is most significantly improved.

[0021] Preferably, in the above-mentioned pollination method for improving the hybridization efficiency of tea trees, the preferred nutrient formulation is: 5g-6g of potassium dihydrogen phosphate, 0.5g-0.6g of cobalamin, and 1L of sterile water.

[0022] Preferably, in the above-mentioned pollination method for improving tea tree hybridization efficiency, the isolation bag is a parchment bag with air holes at the bottom, and a filter pad is filled in the air holes. The air holes are for ventilation, and the filter pad is used to filter impurities such as pollen.

[0023] Preferably, in the above-mentioned pollination method for improving tea tree hybridization efficiency, the filter pad is provided with filter holes, and the filter hole diameter is 0.22 μm to 0.45 μm. If a filter membrane with a filter hole diameter of 0.22 μm to 0.45 μm is used, the filter hole size has a bacteria filtering effect and can also filter out large particles of impurities and pollen. Because the diameter of most pollen is 20 μm to 50 μm.

[0024] Preferably, in the above-mentioned pollination method for improving tea tree hybridization efficiency, the air holes are circular holes with a diameter of 0.5 cm to 1 cm. For example, the number of air holes can be set to two, symmetrically arranged on both sides of the flower.

[0025] The second technical solution of the present invention is as follows:

[0026] A nutrient, the nutrient being the above nutrient, has a formula of: 3g-8g potassium dihydrogen phosphate, 0.05g-0.1g cobalamin, and 1L sterile water.

[0027] The third technical solution of the present invention is as follows:

[0028] The invention discloses an application of a nutrient in tea tree hybrid breeding, wherein the nutrient is used to improve the hybrid success rate and reduce the young fruit shedding rate.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The technical research and development route of the present invention is shown in Figure 1 , designed and obtained excellent nutrient formula, and then combined with appropriate pollination methods, ultimately increased the fruit setting rate of tea trees and reduced the rate of young fruit falling.

[0031] The research of the present invention shows that with the change of the dosage of potassium dihydrogen phosphate and cobalamin, the fruit setting rate and the young fruit shedding rate fluctuate slightly, but the nutrient agent after the two are combined has significantly improved effects than single factors, neutral solution and blank control.

[0032] Research of the present invention shows that after air holes are set at the bottom of the parchment bag, sterile air enters the bag, which helps to better complete the hybridization process, improve the stability of the paternal and maternal genomes after combination, improve the quality of development, increase the fruit setting rate, and reduce the young fruit shedding rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is the technical research and development roadmap of the present invention.

[0034] Figure 2 The present invention is a flowchart of the implementation of the pollination method for improving the hybridization efficiency of tea trees. DETAILED DESCRIPTION

[0035] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below in conjunction with specific embodiments and drawings.

[0036] In the description of the present invention, unless otherwise specified, all reagents used are commercially available and all methods used are conventional techniques in the art.

[0037] Example 1

[0038] A nutrient formulation comprises 3 g of potassium dihydrogen phosphate, 0.05 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0039] Example 2

[0040] A nutrient formulation comprises 3 g of potassium dihydrogen phosphate, 0.06 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0041] Example 3

[0042] A nutrient formulation comprises 3 g of potassium dihydrogen phosphate, 0.07 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0043] Example 4

[0044] A nutrient formulation comprises 3 g of potassium dihydrogen phosphate, 0.08 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0045] Example 5

[0046] A nutrient formulation comprises 3 g of potassium dihydrogen phosphate, 0.09 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0047] Example 6

[0048] A nutrient formulation comprises 3 g of potassium dihydrogen phosphate, 0.1 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0049] Example 7

[0050] A nutrient formulation comprises 4 g of potassium dihydrogen phosphate, 0.06 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0051] Example 8

[0052] A nutrient formulation comprises 5 g of potassium dihydrogen phosphate, 0.06 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0053] Example 9

[0054] A nutrient formulation comprises 6 g of potassium dihydrogen phosphate, 0.06 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0055] Example 10

[0056] A nutrient formulation comprises 7 g of potassium dihydrogen phosphate, 0.06 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0057] Embodiment 11

[0058] A nutrient formulation comprises 8 g of potassium dihydrogen phosphate, 0.06 g of cobalamin, and 1 L of sterile water. The raw materials are mixed to prepare a solution for later use.

[0059] Example 12

[0060] A pollination method for improving tea tree hybridization efficiency, see the implementation flow chart Figure 2 ,include:

[0061] The pollen of the male parent was collected and stored at 4°C for later use. The male parent variety was Anji white tea.

[0062] The female plant is emasculated, and the pollen of the male plant is smeared on the stigma of the emasculated female plant's flower to complete the pollination operation and form a hybrid flower; the female plant is the golden chrysanthemum tea tree variety.

[0063] Hybrid flowers are enclosed in isolation bags.

[0064] The day of pollination was recorded as day 0. On the third day after pollination, the isolation bag was removed, the nutrient agent was applied, and then the hybrid flowers were continued to be wrapped with the isolation bag; 100 μL of the nutrient agent was applied to each hybrid flower to moisten the stigma of the female parent; the isolation bag was a parchment bag; the parchment bag was 10 cm long and 5 cm wide.

[0065] On the 7th day after pollination, completely remove the isolation bag and allow the plants to develop in the natural environment.

[0066] Pollination was carried out using the method of Example 12, and the effects of the nutrient formulations in Examples 1 to 11 were explored respectively. A sterile aqueous solution of potassium dihydrogen phosphate and a sterile aqueous solution of cobalamin were used as single factor controls, and sterile water was used as a blank control to explore the effects of different nutrient formulations on hybridization efficiency. The results are shown in Table 1.

[0067] Among them, the formula of the sterile aqueous solution of potassium dihydrogen phosphate is 3g of potassium dihydrogen phosphate and 1L of sterile water. The raw materials are mixed to prepare a solution for use.

[0068] In addition, the pH of the sterile potassium dihydrogen phosphate aqueous solution was adjusted to a neutral pH of 7.0 using sodium hydroxide, and this nutrient was referred to as a neutral potassium dihydrogen phosphate solution.

[0069] The formula of the sterile aqueous solution of cobalamin is 0.06 g of cobalamin and 1 L of sterile water. The raw materials are mixed to prepare a solution for use.

[0070] In Table 1, the calculation method of fruit setting rate is:

[0071] Fruit setting rate = 100% × (number of hybrid flowers set / number of hybrid flowers)

[0072] The calculation method of young fruit abscission rate is:

[0073] The young fruit shedding rate = 100% × (number of young fruits shedding / number of hybrid flowers fruiting). This method calculates the total number of fruits shedding from fruiting to young fruiting. The number of fruits shedding is no longer counted during the fruit ripening period.

[0074] It should be noted that in the prior art, the growth and development of tea fruit is divided into flowering period, fruiting period, young fruit period and maturity period. Among them, the flowering period is the period of pollination, the fruiting period is the period when the petals fall off and the ovary appears on the tea tree branches, the young fruit period is the period when the ovary grows to a fixed shape and size, and the maturity period is the period when the fruit is colored and grows to a mature fruit. It takes several months for tea fruit to grow from pollination to maturity. The present invention is the fruit setting rate calculated at the end of the fruiting period, and the young fruit shedding rate calculated at the end of the young fruit period.

[0075] It should also be noted that, except for the different nutrient formulations, the pollination methods and subsequent field management methods of the groups in Table 1 were the same, eliminating the interference of factors other than nutrients on the experimental results.

[0076] The control pollination method was performed as follows:

[0077] A pollination method for improving tea tree hybridization efficiency, comprising:

[0078] The pollen of the male parent was collected and stored at 4°C for later use. The male parent variety was Anji white tea.

[0079] The female plant is emasculated, and the pollen of the male plant is smeared on the stigma of the emasculated female plant's flower to complete the pollination operation and form a hybrid flower; the female plant is the golden chrysanthemum tea tree variety.

[0080] The hybrid flowers are wrapped in an isolation bag; the isolation bag is a parchment bag; the parchment bag is 10 cm long and 5 cm wide.

[0081] On the 7th day after pollination, completely remove the isolation bag and allow the plants to develop in the natural environment.

[0082] Table 1 Effect of nutrient formulation

[0083]

[0084]

[0085] The results in Table 1 show that with the change of the dosage of potassium dihydrogen phosphate and cobalamin, the fruit setting rate and young fruit drop rate fluctuated slightly, but the combined nutrient agent of the two significantly improved the effect compared with the single factor, neutral solution and blank control.

[0086] Based on the experimental results in Table 1, we explored the effects of different pollination methods on hybridization efficiency based on the nutrient formulation of Example 8, which had the best effect.

[0087] Example 13

[0088] A pollination method for improving tea tree hybridization efficiency, comprising:

[0089] The pollen of the male parent was collected and stored at 4°C for later use. The male parent variety was Anji white tea.

[0090] The female plant is emasculated, and the pollen of the male plant is smeared on the stigma of the emasculated female plant's flower to complete the pollination operation and form a hybrid flower; the female plant is the golden chrysanthemum tea tree variety.

[0091] Hybrid flowers are enclosed in isolation bags.

[0092] The day of pollination was recorded as day 0. On the third day after pollination, the isolation bag was removed, nutrients were applied, and then the isolation bag was continued to be used to wrap the hybrid flowers; 150 μL of nutrients were applied to each hybrid flower; the isolation bag was a parchment bag; the parchment bag was 10 cm long and 5 cm wide.

[0093] On the 7th day after pollination, completely remove the isolation bag and allow the plants to develop in the natural environment.

[0094] Embodiment 14

[0095] A pollination method for improving tea tree hybridization efficiency, comprising:

[0096] The pollen of the male parent was collected and stored at 4°C for later use. The male parent variety was Anji white tea.

[0097] The female plant is emasculated, and the pollen of the male plant is smeared on the stigma of the emasculated female plant's flower to complete the pollination operation and form a hybrid flower; the female plant is the golden chrysanthemum tea tree variety.

[0098] Hybrid flowers are enclosed in isolation bags.

[0099] The day of pollination was recorded as day 0. On the third day after pollination, the isolation bag was removed, nutrients were applied, and then the hybrid flowers were continued to be wrapped with the isolation bag; 200 μL of nutrients were applied to each hybrid flower; the isolation bag was a parchment bag; the parchment bag was 10 cm long and 5 cm wide.

[0100] On the 7th day after pollination, completely remove the isolation bag and allow the plants to develop in the natural environment.

[0101] Embodiment 15

[0102] A pollination method for improving tea tree hybridization efficiency, comprising:

[0103] The pollen of the male parent was collected and stored at 4°C for later use. The male parent variety was Anji white tea.

[0104] The female plant is emasculated, and the pollen of the male plant is smeared on the stigma of the emasculated female plant's flower to complete the pollination operation and form a hybrid flower; the female plant is the golden chrysanthemum tea tree variety.

[0105] Hybrid flowers are enclosed in isolation bags.

[0106] The day of pollination was recorded as day 0. On the third day after pollination, the isolation bag was removed, nutrients were applied, and then the isolation bag was continued to be used to wrap the hybrid flowers; 250 μL of nutrients were applied to each hybrid flower; the isolation bag was a parchment bag; the parchment bag was 10 cm long and 5 cm wide.

[0107] On the 7th day after pollination, completely remove the isolation bag and allow the plants to develop in the natural environment.

[0108] Example 16

[0109] A pollination method for improving tea tree hybridization efficiency, comprising:

[0110] The pollen of the male parent was collected and stored at 4°C for later use. The male parent variety was Anji white tea.

[0111] The female plant is emasculated, and the pollen of the male plant is smeared on the stigma of the emasculated female plant's flower to complete the pollination operation and form a hybrid flower; the female plant is the golden chrysanthemum tea tree variety.

[0112] Hybrid flowers are enclosed in isolation bags.

[0113] The day of pollination was recorded as day 0. On the third day after pollination, the isolation bag was removed, nutrients were applied, and then the isolation bag was continued to be used to wrap the hybrid flowers; 300 μL of nutrients were applied to each hybrid flower; the isolation bag was a parchment bag; the parchment bag was 10 cm long and 5 cm wide.

[0114] On the 7th day after pollination, completely remove the isolation bag and allow the plants to develop in the natural environment.

[0115] Embodiment 17

[0116] A pollination method for improving tea tree hybridization efficiency, comprising:

[0117] The pollen of the male parent was collected and stored at 4°C for later use. The male parent variety was Anji white tea.

[0118] The female plant is emasculated, and the pollen of the male plant is smeared on the stigma of the emasculated female plant's flower to complete the pollination operation and form a hybrid flower; the female plant is the golden chrysanthemum tea tree variety.

[0119] Hybrid flowers are enclosed in isolation bags.

[0120] The day of pollination was recorded as day 0. On the 5th day after pollination, the isolation bag was removed, the nutrient agent was applied, and then the hybrid flowers were continued to be wrapped with the isolation bag; 100 μL of nutrient agent was applied to each hybrid flower; the isolation bag was a parchment bag; the parchment bag was 10 cm long and 5 cm wide.

[0121] On the 9th day after pollination, the isolation bag is completely removed and the plants are allowed to develop in the natural environment.

[0122] Embodiment 18

[0123] A pollination method for improving tea tree hybridization efficiency, comprising:

[0124] The pollen of the male parent was collected and stored at 4°C for later use. The male parent variety was Anji white tea.

[0125] The female plant is emasculated, and the pollen of the male plant is smeared on the stigma of the emasculated female plant's flower to complete the pollination operation and form a hybrid flower; the female plant is the golden chrysanthemum tea tree variety.

[0126] Hybrid flowers are enclosed in isolation bags.

[0127] The day of pollination was recorded as day 0. On the third day after pollination, the isolation bag was opened, the nutrient agent was applied, and then the isolation bag was continued to be used to wrap the hybrid flowers; 100 μL of the nutrient agent was applied to each hybrid flower;

[0128] The isolation bag is a parchment bag; the parchment bag is 10 cm long and 5 cm wide. A ventilation hole is provided at the bottom, and the height of the ventilation hole from the bottom of the flower is 2 cm. A filter pad is filled in the ventilation hole. The filter pad is provided with filter holes, and the diameter of the filter holes is 0.22 μm. The ventilation holes are circular holes with a diameter of 1 cm.

[0129] On the 7th day after pollination, completely remove the isolation bag and allow the plants to develop in the natural environment.

[0130] Embodiment 19

[0131] A pollination method for improving tea tree hybridization efficiency, comprising:

[0132] The pollen of the male parent was collected and stored at 4°C for later use. The male parent variety was Anji white tea.

[0133] The female plant is emasculated, and the pollen of the male plant is smeared on the stigma of the emasculated female plant's flower to complete the pollination operation and form a hybrid flower; the female plant is the golden chrysanthemum tea tree variety.

[0134] Hybrid flowers are enclosed in isolation bags.

[0135] The day of pollination was recorded as day 0. On the third day after pollination, the isolation bag was removed, the nutrient agent was applied, and then the isolation bag was continued to be used to wrap the hybrid flowers; 100 μL of the nutrient agent was applied to each hybrid flower;

[0136] The isolation bag is a parchment bag; the parchment bag is 10 cm long and 5 cm wide. A ventilation hole is provided at the bottom, and the height of the ventilation hole from the bottom of the flower is 2 cm. A filter pad is filled in the ventilation hole. The filter pad is provided with a filter hole, and the filter hole diameter is 0.45 μm. The ventilation hole is a circular hole with a diameter of 0.5 cm.

[0137] On the 7th day after pollination, completely remove the isolation bag and allow the plants to develop in the natural environment.

[0138] Table 2 Effects of different pollination methods

[0139] Grouping Fruit setting rate (%) Young fruit drop rate (%) Example 12 36 41 Embodiment 13 38 39 Embodiment 14 39 39 Embodiment 15 40 39 Example 16 36 41 Embodiment 17 36 40 Embodiment 18 45 33 Embodiment 19 43 35 Sterile water, method of Example 12 21 60

[0140] The results in Table 2 show that after air holes are set at the bottom of the parchment bag, sterile air enters the bag, which helps to better complete the hybridization process, improve the stability of the paternal and maternal genomes after combination, improve the quality of development, increase the fruit setting rate, and reduce the young fruit shedding rate.

[0141] Comprehensively comparing the data in Table 1 and Table 2, the best nutrient is that in Example 8, and the best pollination method is that in Example 18.

[0142] It should be noted that when the present invention involves a numerical range, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods used are the same as those in the embodiments, in order to avoid redundancy, the present invention describes a preferred embodiment. Although the preferred embodiments of the present invention have been described, once those skilled in the art know the basic creative concept, they can make additional changes and modifications to these embodiments, and these changes and modifications all fall within the scope of the present invention.

Claims

1. A pollination method for improving tea tree hybridization efficiency, characterized in that: include: Collect male parent pollen; The female plant is emasculated, and the pollen of the male plant is applied to the stigma of the emasculated female plant flower to complete the pollination operation and form a hybrid flower; Isolation bags wrap hybrid flowers; The day of pollination is recorded as day 0. On the 3rd to 5th day after pollination, the isolation bag is removed, the nutrient agent is applied, and then the hybrid flowers are continued to be wrapped with the isolation bag; The formula of the nutrient is: 3g-8g potassium dihydrogen phosphate, 0.05g-0.1g cobalamin, 1L sterile water; the application amount is to wet the stigma of the mother plant; On the 7th to 9th day after pollination, the isolation bag is completely removed and the plants are allowed to develop in the natural environment.

2. The pollination method for improving tea tree hybridization efficiency according to claim 1, characterized in that: Apply 100 μL to 300 μL of nutrient solution to each hybrid flower.

3. The pollination method for improving tea tree hybridization efficiency according to claim 1, characterized in that: Apply 150 μL to 250 μL of nutrient solution to each hybrid flower.

4. The pollination method for improving tea tree hybridization efficiency according to claim 2, characterized in that: 72h to 96h after applying the nutrient, remove the isolation bag.

5. The pollination method for improving tea tree hybridization efficiency according to claim 1, characterized in that: The formula of the nutrient is: 5g-6g of potassium dihydrogen phosphate, 0.5g-0.6g of cobalamin, and 1L of sterile water.

6. The pollination method for improving tea tree hybridization efficiency according to claim 1, characterized in that: The isolation bag is a parchment paper bag with air holes at the bottom, and the air holes are filled with filter pads.

7. The pollination method for improving tea tree hybridization efficiency according to claim 6, characterized in that: The filter pad is provided with filter holes, and the diameter of the filter holes is 0.22 μm to 0.45 μm.

8. The pollination method for improving tea tree hybridization efficiency according to claim 6, characterized in that: The ventilation holes are circular holes with a diameter of 0.5 cm to 1 cm.

9. A nutritional supplement, characterized in that The nutrient is the nutrient according to claim 1, and the nutrient is used for tea tree hybridization.

10. Use of the nutrient according to claim 9 in tea tree hybrid breeding, characterized in that: The nutrient agent is used for improving the hybridization success rate and reducing the young fruit shedding rate.

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