Preparation method of fluorescent rodenticide

Abstract

The invention relates to the technical field of pesticides, in particular to a preparation method of a fluorescent rodenticide, raw materials of the fluorescent rodenticide comprise an active component, a carrier and a fluorescent agent, the rodenticide emits fluorescent colors in the dark, and the encountering property and the feeding rate of rats and the rodenticide are enhanced. Under the irradiation of weak light or ultraviolet light and infrared light, the drug delivery site is quickly sensed, residual fluorescent residue particles after the mouse takes the drug are found, and the mouse hiding site can be quickly found and tracked through the scattered particles, so that the problem of damage caused by rats can be thoroughly controlled and eliminated from the source. Therefore, the spreading of diseases is reduced, and the market vacancy is filled.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and in particular to a method for preparing fluorescent rat poison. Background Art

[0002] Rodents are small, have a varied diet, can adapt to a variety of harsh environmental conditions, and have a strong ability to generalize, making them the largest group of mammals. At present, rodent infestation has had a huge negative impact on human production and life. It not only reduces agricultural output, consumes a large amount of agricultural and sideline products, and destroys and consumes industrial and water conservancy facilities, but also poses a serious threat to forestry, grassland and other ecosystems. Rodents spread diseases, pollute the environment, and threaten human health and life safety. Rodents can spread more than 30 diseases to humans, including plague, hemorrhagic fever, leptospirosis and other extremely harmful diseases, which seriously threaten human health. At the same time, rodents are also carriers and transmitters of African swine fever virus and are the natural enemies of breeding companies.

[0003] At present, rodenticides have a single function, and they are all bait-type varieties. Users actively feed the bait, but it is difficult to find and track the hiding places of rats, so it is impossible to completely control and eliminate the rodent problem from the source. Therefore, the market urgently needs an economical and convenient product that can attract rats, kill rats, and track the hiding places of rats. Summary of the invention

[0004] In view of this, the present invention provides a method for preparing fluorescent mouse poison, which improves the encounterability and feeding rate of mice with the drug by adding a fluorescent agent.

[0005] The technical solution of the present invention is implemented as follows: the present invention provides a fluorescent mouse poison, the raw materials of which include: an active ingredient, a carrier and a fluorescent agent.

[0006] In some embodiments, the amount of the fluorescent agent is 0.1%-20% by weight of the carrier.

[0007] In some embodiments, the active ingredient includes at least one of an anticoagulant, a neurotoxic agent, a metabolic inhibitor, a botulinum toxin, a plant toxin, and vitamin D3.

[0008] In some embodiments, the anticoagulant includes: at least one of warfarin, bromadiolone, and fludioxonil; the neurotoxic agent includes: at least one of cypermethrin and tetramine; the metabolic inhibitor includes: at least one of fluthiol and fluoroacetic acid; the plant toxin includes: triptolide and ricin.

[0009] In some embodiments, the dosage of the active ingredient varies depending on the type of active ingredient. Specifically, when an anticoagulant is used, the dosage is 0.005%-0.5% of the weight of the carrier; when a neurotoxic agent is used, the dosage is 0.0025%-0.5% of the weight of the carrier; when a metabolic inhibitor is used, the dosage is 0.01%-0.5% of the weight of the carrier; when a plant toxin is used, the dosage is 0.0001%-0.5% of the weight of the carrier.

[0010] In some embodiments, the carrier includes at least one of: cereal flour, vegetable oil, animal oil, sugar, milk powder, honey, starch, gelatin, cellulose, polymer, clay, gypsum powder, activated carbon, water, glycerin, syrup, and wax.

[0011] In some embodiments, the fluorescent agent includes at least one of an infrared fluorescent agent, an ultraviolet fluorescent agent, a near-infrared fluorescent agent, and a long-wave fluorescent agent.

[0012] In some embodiments, the infrared fluorescent agent includes at least one of IR-780, IR-800, IR-830, Cy5.5, and Cy7; the ultraviolet fluorescent agent includes at least one of DAPI, Acridine Orange, and Hoechst 33342; the near-infrared fluorescent agent includes at least one of Indocyanine Green and QD fluorescent agent; and the long-wave fluorescent agent includes at least one of Alexa Fluor 680 and Alexa Fluor 750.

[0013] The preparation method of the fluorescent mouse poison of the invention comprises the steps of uniformly mixing active ingredients, carriers and fluorescent agents, and granulating to obtain the fluorescent mouse poison.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The invention adds a fluorescent agent to conventional rat poison, so that the rat poison emits fluorescent color in the dark, thereby increasing the attraction and encounterability of the rat poison to rats and improving the feeding rate of rats. At the same time, it is also helpful for the person administering the poison to quickly find the rat poison residues left after the rats have eaten, which is convenient for cleaning and tracking the hiding places of the rats, thereby completely controlling and eliminating the rat problem from the source. DETAILED DESCRIPTION

[0016] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the embodiments of the present invention belong. If the definitions set forth in this section are contrary to or otherwise inconsistent with the definitions set forth in the patents, patent applications, published patent applications, and other publications incorporated herein by reference, the definitions listed in this section take precedence over the definitions incorporated herein by reference.

[0018] Unless otherwise specified, the methods used in the following examples are all conventional methods. Unless otherwise specified, the materials, reagents and instruments used are all conventional materials, reagents and instruments in the art, and can be obtained by those skilled in the art through commercial channels.

[0019] When an amount, concentration or other value or parameter is expressed as a range, a preferred range or a range defined by a series of upper preferred values ​​and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted as including the range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described herein, unless otherwise stated, the range is intended to include its end values ​​and all integers and fractions within the range. In the present specification and claims, range definitions can be combined and / or interchanged, and if not otherwise stated, these ranges include all subranges contained therein.

[0020] In order to verify whether the fluorescent rodenticide provided by the present invention has a better effect of killing rats by taking food, the following examples and comparative examples are now described in detail.

[0021] Example 1

[0022] This embodiment provides a wax block fluorescent rat poison

[0023] The raw materials include: 800g of cereal powder, 100g of milk powder, 100g of paraffin, 40g of fluorescent agent IR780, and 0.05g of bromadiolone.

[0024] Preparation of fluorescent rat poison: Mix grain powder, milk powder, fluorescent agent IR780 and bromadiolone evenly, then heat the paraffin to 65°C to obtain liquid paraffin. Mix the liquid paraffin with other raw materials and add it to a pill making machine to make pills.

[0025] Example 2

[0026] On the basis of Example 1, other conditions were kept unchanged, and the only difference was that the feeding amount of the fluorescent agent IR780 was adjusted to 60 g.

[0027] Example 3

[0028] On the basis of Example 1, other conditions were kept unchanged, and the only difference was that the feeding amount of the fluorescent agent IR780 was adjusted to 100 g.

[0029] Example 4

[0030] On the basis of Example 1, other conditions were kept unchanged, and the only difference was that the feeding amount of the fluorescent agent IR780 was adjusted to 20 g.

[0031] Example 5

[0032] On the basis of Example 1, other conditions were kept unchanged, and the only difference was that the feeding amount of the fluorescent agent IR780 was adjusted to 1 g.

[0033] Example 6

[0034] On the basis of Example 1, other conditions were kept unchanged, and the only difference was that the feeding amount of fluorescent agent IR780 was adjusted to 150 g.

[0035] Example 7

[0036] On the basis of Example 1, other conditions remain unchanged, and the only difference is that the feeding amount of fluorescent agent IR780 is adjusted to 200 g.

[0037] Comparative Example 1

[0038] On the basis of Example 1, other conditions remain unchanged, and the only difference is that the fluorescent agent is replaced with an equal mass of cereal flour.

[0039] The rat poisons of the above-mentioned embodiments and comparative examples were tested. The purpose of the experiment was to observe the feeding and death of the test animals on ordinary rat poisons and rat poisons with added fluorescent agents, so as to obtain the data on the reference dose of fluorescent agents added in the test samples.

[0040] Test location: light-tight basement (no water source), area: about 30m 2 ;

[0041] Test environment conditions: temperature 20±2℃, relative humidity 50%±4%;

[0042] Experimental animals: SD rats 200 g ± 20 g; 10 target mice, half male and half female, fasted overnight.

[0043] Test lighting: 3W LED bulb, fixed on the ceiling of the basement, controlled by a timer switch, turned on once every 4 hours / 5 seconds each time to supplement the light source for the fluorescent agent.

[0044] Test time: 5 days.

[0045] Test method:

[0046] Place four 30*20*30 square empty yellow cardboard boxes in the four corners of the basement, leaving a 5 cm gap between the boxes and the corners. On the two sides of the boxes facing the wall, make a 5 cm high and 10 cm long rectangular opening in the middle to simulate a rat's nest.

[0047] Placement requirements:

[0048] The baits were weighed before the test and arranged in a tic-tac-toe pattern at 8 locations in the room, with a 20*30 yellow cardboard piece placed on each location.

[0049] The samples include 8 groups in total, including Examples 1-7 and Comparative Example 1, each group is taken as 1 portion, and each portion weighs 100 g.

[0050] Placement: Arrange the poison baits of the embodiment group and the control group in a tic-tac-toe pattern, evenly space them on each yellow cardboard, and mark the corresponding groups.

[0051] Sample Collection Records:

[0052] At the end of the test, remove the dead rats, check and record the consumption of each point, collect the remaining poison baits scattered on the paper and weigh them.

[0053] After 5 days, i.e. 120 hours, the feeding coefficient and the mortality rate of the test mice were used to evaluate the feeding situation of the rat poison with different contents of fluorescent agent, the influence and effect of the attracting and attractiveness of the fluorescent poison bait in the dark.

[0054] This test refers to the method used

[0055] Partial classification of "GB / T15670.2-2017 Toxicological test methods for pesticide registration Part 2: Acute oral toxicity test Horn's method"; Judgment: The feeding rate of test animals on ordinary rodent poison: 53.2%, mortality: 100%; The feeding rate of test animals on fluorescent rodent poison: 77.3%, mortality: 100%.

[0056] Compared with the rat poison without fluorescent agent, the feeding rate of the rat poison with fluorescent agent was increased to 24.1%. Under the light, a number of fine luminous particles and luminous powder were visible on the ground. There were four rat nests connected to the corners of the wall intermittently. The fluorescent rat poison stored in the rat nests was significantly more than that of ordinary rat poison.

[0057] The statistical results are as follows:

[0058]

[0059] The fluorescent rat poison of the present invention emits fluorescent colors in the dark, which enhances the attraction and encounterability of rats and the drug, and improves the feeding rate of rats. Under the irradiation of weak light or ultraviolet light or infrared light, the drug delivery location can be quickly sensed, and the fluorescent residue particles left after the rats feed can be found. The scattered particles can be used to quickly find and track the hiding places of the rats, thereby completely controlling and eliminating the rat problem from the source.

[0060] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fluorescent rat poison, characterized in that: The raw materials of the fluorescent rat poison include active ingredients, carriers and fluorescent agents.

2. The fluorescent rat poison as claimed in claim 1, characterized in that: The dosage of the fluorescent agent is 0.1%-20% of the weight of the carrier.

3. The fluorescent rat poison as claimed in claim 1, characterized in that: The active ingredients include at least one of anticoagulants, neurotoxic agents, Clostridium botulinum toxin, metabolic inhibitors, plant toxins, and vitamin D3.

4. The fluorescent rat poison as claimed in claim 3, characterized in that: The anticoagulant includes at least one of warfarin, bromadiolone and fludioxonil; the neurotoxic agent includes at least one of cypermethrin and tetramine; the metabolic inhibitor includes at least one of fluthiol and fluoroacetic acid; the plant toxin includes triptolide and ricin.

5. The fluorescent rat poison as claimed in claim 1, characterized in that: The carrier comprises at least one of cereal powder, vegetable oil, animal oil, sugar, milk powder, honey, starch, gelatin, cellulose, polymer, clay, gypsum powder, activated carbon, water, glycerin, syrup and wax.

6. The fluorescent rat poison as claimed in claim 1, characterized in that: The fluorescent agent includes at least one of an infrared fluorescent agent, an ultraviolet fluorescent agent, a near-infrared fluorescent agent, and a long-wave fluorescent agent.

7. The fluorescent rat poison according to claim 6, characterized in that: The infrared fluorescent agent includes at least one of IR-780, IR-800, IR-830, Cy5.5, and Cy7; the ultraviolet fluorescent agent includes at least one of DAPI, Acridine Orange, and Hoechst33342; the near-infrared fluorescent agent includes at least one of Indocyanine Green and QD fluorescent agent; and the long-wave fluorescent agent includes at least one of Alexa Fluor 680 and Alexa Fluor 750.

8. The method for preparing the fluorescent rat poison according to any one of claims 1 to 7, characterized in that: The method comprises the following steps: uniformly mixing active ingredients, carriers and fluorescent agents, and granulating to obtain fluorescent mouse poison.

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