Nanocrystallized oyster shell superfine powder composition spray and preparation method thereof

Abstract

The invention belongs to the field of oral ulcer sprays, and provides a nanocrystallized oyster shell superfine powder composition spray and a preparation method thereof, the nanocrystallized oyster shell superfine powder composition spray comprises the following components: nanocrystallized oyster shell superfine powder, a propolis extract, a honeysuckle extract, menthol, sodium hyaluronate, a stabilizer, a dispersant, purified water, an epidermal growth factor and a natural vitamin E derivative; the nano oyster shell superfine powder, the propolis extract and the honeysuckle flower extract are added and matched with one another, and act on oral ulcer in multiple aspects of resisting inflammation, resisting bacteria, promoting cell repair and the like, so that the healing time is remarkably shortened, and the pain of a patient is relieved; wherein the epidermal growth factor and the natural vitamin E derivative bring unique advantages to the composition, stimulate cell proliferation and differentiation and accelerate the healing process; the antioxidation and circulation promotion effects of the natural vitamin E derivative further improve the treatment effect and reduce scar formation.

Description

Technical Field

[0001] The invention belongs to the field of oral ulcer sprays, in particular to a nano-sized oyster shell ultrafine powder composition spray and a preparation method thereof. Background Art

[0002] Oral ulcer is a common oral mucosal disease that troubles many patients. Its incidence rate accounts for a certain proportion in different populations. The causes of oral ulcer are complex and diverse, including local mechanical damage, such as improper brushing, inappropriate dentures, etc.; nutritional deficiencies, especially deficiencies of B vitamins, vitamin C, zinc and other trace elements; endocrine disorders caused by excessive mental stress; and systemic factors such as immune system disorders. These factors can lead to an imbalance in the damage and repair of oral mucosal epithelial cells, thereby causing oral ulcers.

[0003] Traditional oral ulcer treatment drugs mostly use patches, which have poor adhesion and uneven drug release. Mouth rinses have a short effect time and are easily diluted, so the ulcer takes a long time to heal.

[0004] To this end, those skilled in the art have proposed a nano-oyster shell ultrafine powder composition spray and a preparation method thereof to solve the problems raised by the background technology. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a nano-oyster shell ultrafine powder composition spray and a preparation method thereof, so as to solve the problems in the prior art of poor adhesion of oral ulcer patches, uneven drug release, short action time of gargles and easy dilution, and long ulcer healing time.

[0006] A nano-oyster shell ultrafine powder composition spray comprises the following components: nano-oyster shell ultrafine powder, propolis extract, honeysuckle extract, menthol, sodium hyaluronate, stabilizer, dispersant, purified water, epidermal growth factor (EGF) and natural vitamin E derivatives.

[0007] Preferably, the composition comprises, by weight: 40-50 parts of nano-oyster shell ultrafine powder, 5-10 parts of propolis extract, 5-10 parts of honeysuckle extract, 2-5 parts of menthol, 2-5 parts of sodium hyaluronate, 1-3 parts of stabilizer, 1-3 parts of dispersant, 20-30 parts of purified water, 0.5-2 parts of epidermal growth factor (EGF) and 0.5-2 parts of natural vitamin E derivative.

[0008] Preferably, the composition comprises, by weight: 45 parts of the nano-oyster shell ultrafine powder, 8 parts of propolis extract, 7 parts of honeysuckle extract, 3 parts of menthol, 3 parts of sodium hyaluronate, 2 parts of stabilizer, 2 parts of dispersant, 25 parts of purified water, 1 part of epidermal growth factor, and 1 part of natural vitamin E derivative.

[0009] Preferably, the stabilizer is a complex of sodium alginate and chitosan.

[0010] Preferably, the dispersant is an amphiphilic polymer, the component of which is a polyethylene glycol-polypropylene glycol-polyethylene glycol (PEG-PPG-PEG) triblock copolymer.

[0011] A method for preparing a nano-oyster shell ultrafine powder composition spray, the method comprising the following steps:

[0012] S1. Select and clean the oyster shells, and dry them in an oven at 80-100°C for 4-6 hours until they are completely dry;

[0013] The dried oyster shells are crushed into coarse particles by a coarse crusher, and then crushed by an air flow crusher at an air flow pressure of 0.6-0.8 MPa for 30-60 minutes to obtain oyster shell ultrafine powder;

[0014] The oyster shell ultrafine powder is placed in a nano grinder, 0.5% to 1% of the mass of the dispersant is added, and the powder is ground for 2 to 4 hours to obtain nano-sized oyster shell ultrafine powder with an average particle size of 50 to 200 nm;

[0015] S2, freezing the propolis raw material at -5-5°C for 24-48 hours to harden and brittle it; after breaking it into small pieces, adding 70%-90% edible ethanol solution at a mass ratio of 1:5-1:10, ultrasonically extracting it in a sealed container at 200-300W ultrasonic power for 30-60 minutes, and extracting it 2-3 times; combining the extracts, removing ethanol by vacuum distillation, and drying the obtained concentrate in a vacuum drying oven at 40-60°C for 4-6 hours to obtain a propolis extract;

[0016] S3, take honeysuckle medicinal material, grind it into coarse powder with a grinder; add purified water according to the corresponding mass ratio, heat and reflux to extract for 1-2 hours, and extract 2-3 times; combine the extracts, filter and concentrate under reduced pressure to an extract with a relative density of 1.1-1.3 (60°C), and then vacuum dry to obtain a honeysuckle extract;

[0017] S4, dissolving epidermal growth factor (EGF) and natural vitamin E derivative in phosphate buffered saline (PBS) respectively to prepare a solution with a concentration of 1-5 mg / ml for standby use;

[0018] S5. Take an appropriate amount of pure water, slowly add the nano-oyster shell ultrafine powder, and stir at a high speed of 800-1200r / min for 20-30 minutes to form a suspension;

[0019] Add propolis extract, honeysuckle extract, menthol and sodium hyaluronate to the remaining purified water in sequence, and heat and stir in a water bath (400-600r / min) until completely dissolved or evenly dispersed;

[0020] Add stabilizer and dispersant, continue stirring for 30-60 minutes, then slowly add pre-prepared epidermal growth factor (EGF) and natural vitamin E derivative solution, and stir evenly;

[0021] Finally, the mixture is filtered and sterilized through a 0.22-0.45 μm microporous filter membrane, and the mixture is filled into a spray bottle to obtain a nano-oyster shell ultrafine powder composition spray.

[0022] Preferably, the ratio of the honeysuckle crude powder to purified water is 1:8-1:12.

[0023] Preferably, the water bath temperature in S5 is 40-60°C.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The present invention adds nano-oyster shell ultrafine powder, propolis extract, and honeysuckle extract to cooperate with each other, and jointly acts on oral ulcers from multiple aspects such as anti-inflammatory, antibacterial, and promoting cell repair, thereby significantly shortening the healing time and alleviating the patient's pain; among them, epidermal growth factor and natural vitamin E derivatives bring unique advantages to the composition, stimulating cell proliferation and differentiation and accelerating the healing process; the antioxidant and circulation-promoting effects of natural vitamin E derivatives further enhance the therapeutic effect and reduce scar formation. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the overall structure of the present invention. DETAILED DESCRIPTION

[0027] The following is a further detailed description of the embodiments of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0028] As attached Figure 1 As shown: The present invention provides a nano-oyster shell ultrafine powder composition spray, comprising the following components: nano-oyster shell ultrafine powder, propolis extract, honeysuckle extract, menthol, sodium hyaluronate, stabilizer, dispersant, purified water, epidermal growth factor (EGF) and natural vitamin E derivatives.

[0029] The composition includes, by weight: 40-50 parts of nano-oyster shell ultrafine powder, 5-10 parts of propolis extract, 5-10 parts of honeysuckle extract, 2-5 parts of menthol, 2-5 parts of sodium hyaluronate, 1-3 parts of stabilizer, 1-3 parts of dispersant, 20-30 parts of purified water, 0.5-2 parts of epidermal growth factor (EGF) and 0.5-2 parts of natural vitamin E derivative;

[0030] Epidermal growth factor (EGF) uses recombinant human epidermal growth factor (rhEGF). Recombinant human epidermal growth factor (rhEGF) is a protein synthesized by genetic engineering. The gene sequence is derived from the human epidermal growth factor gene. The gene is introduced into a suitable host cell (such as Escherichia coli or mammalian cells), allowing the host cell to synthesize a protein with the same structure and function as the natural human epidermal growth factor according to the gene information;

[0031] The natural vitamin E derivative uses α-tocopherol acetate. In the inflammatory environment of oral ulcers, a large number of free radicals will be generated. α-tocopherol acetate can slowly release α-tocopherol locally in the oral mucosa and use its phenolic hydroxyl structure to remove free radicals, such as superoxide anion free radicals, hydroxyl free radicals and lipid peroxide free radicals. It can reduce the damage of oxidative stress to oral mucosal cells and protect the integrity of cells, thus providing favorable conditions for the healing of oral ulcers.

[0032] α-Tocopherol acetate can regulate the signal transduction pathways within cells and reduce the production of inflammatory mediators (such as prostaglandin E2, etc.), thereby alleviating the inflammatory response of oral ulcers. At the same time, it can promote local blood circulation, and work together with sodium hyaluronate and other ingredients to improve the nutrient supply of the wound surface, help tissue repair and regeneration, and further accelerate the healing process of oral ulcers.

[0033] 45 parts of nano-oyster shell ultrafine powder, 8 parts of propolis extract, 7 parts of honeysuckle extract, 3 parts of menthol, 3 parts of sodium hyaluronate, 2 parts of stabilizer, 2 parts of dispersant, 25 parts of purified water, 1 part of epidermal growth factor, and 1 part of natural vitamin E derivative.

[0034] The stabilizer is a complex of sodium alginate and chitosan; the dispersant is an amphiphilic polymer, the component of which is a polyethylene glycol-polypropylene glycol-polyethylene glycol (PEG-PPG-PEG) triblock copolymer.

[0035] The preparation method comprises the following steps:

[0036] S1. Preparation of nano-oyster shell ultrafine powder: Select high-quality oyster shells and wash them, wash the surface impurities with clean water, and dry them in an oven at 80-100°C for 4-6 hours until they are completely dry;

[0037] The dried oyster shells are crushed into coarse particles by a coarse crusher, and then crushed by a jet mill at an airflow pressure of 0.6-0.8 MPa for 30-60 minutes to obtain oyster shell ultrafine powder;

[0038] The oyster shell ultrafine powder is placed in a nano grinder, 0.5% to 1% of the mass of the dispersant is added, and the powder is ground for 2 to 4 hours to obtain nano-sized oyster shell ultrafine powder with an average particle size of 50 to 200 nm;

[0039] S2, preparation of propolis extract; the propolis raw material is frozen at -5-5°C for 24-48 hours to harden and become brittle; after breaking into small pieces, 70%-90% edible ethanol solution is added at a mass ratio of 1:5-1:10, and ultrasonic extraction is performed in a sealed container at 200-300W ultrasonic power for 30-60 minutes, and extraction is performed 2-3 times; the extracts are combined, and the ethanol is removed by vacuum distillation, and the obtained concentrate is dried in a vacuum drying oven at 40-60°C for 4-6 hours to obtain the propolis extract;

[0040] S3, preparation of honeysuckle extract; taking honeysuckle medicinal materials, crushing them into coarse powder with a grinder; adding purified water at a mass ratio of 1:8-1:12, heating and refluxing for extraction for 1-2 hours, and extracting 2-3 times; combining the extracts, filtering, and concentrating under reduced pressure to an extract with a relative density of 1.1-1.3 (60° C.), and then vacuum drying to obtain the honeysuckle extract;

[0041] S4, treatment of epidermal growth factor (EGF) and natural vitamin E derivatives: dissolving epidermal growth factor (EGF) and natural vitamin E derivatives in a small amount of phosphate buffered saline (PBS) to prepare a solution with a concentration of 1-5 mg / ml for standby use;

[0042] S5, preparation of the composition: take an appropriate amount of purified water, slowly add the nano-oyster shell ultrafine powder, and stir at a high speed of 800-1200r / min for 20 to 30 minutes to form a suspension;

[0043] Add propolis extract, honeysuckle extract, menthol and sodium hyaluronate to the remaining purified water in sequence, heat and stir (400-600r / min) in a water bath at 40-60°C until completely dissolved or evenly dispersed;

[0044] Add stabilizer and dispersant, continue stirring for 30 to 60 minutes, then slowly add pre-prepared epidermal growth factor (EGF) and natural vitamin E derivative solution, and stir evenly;

[0045] Finally, the mixture is filtered and sterilized through a 0.22-0.45 μm microporous filter membrane, and the mixture is filled into a spray bottle to obtain a nano-oyster shell ultrafine powder composition spray.

[0046] Embodiment 1:

[0047] S1. Preparation of nano-oyster shell ultrafine powder: 100 g of high-quality oyster shells were selected, washed and dried in an oven at 90° C. for 5 hours; after coarse crushing, ultrafine powder was obtained by crushing with a jet mill at a pressure of 0.7 MPa for 45 minutes; 0.75 g of stearic acid was added, and the mixture was ground in a nano-grinder for 3 hours to obtain 45 g of nano-oyster shell ultrafine powder with an average particle size of about 100 nm.

[0048] S2. Preparation of propolis extract: 20 g of propolis raw material was frozen at 0°C for 36 hours and then crushed; 120 g of 80% edible ethanol solution was added, and ultrasonic extraction was performed at 250 W ultrasonic power for 45 minutes, and the extraction was repeated 3 times; the combined extracts were distilled under reduced pressure to remove ethanol, and vacuum dried at 45°C for 5 hours to obtain 8 g of propolis extract.

[0049] S3. Preparation of honeysuckle extract: 50 g of honeysuckle was crushed into coarse powder, 400 g of purified water was added, and the mixture was heated under reflux for extraction for 1.5 hours, and the extraction was repeated 3 times; the combined extracts were filtered and concentrated under reduced pressure to an extract with a relative density of 1.2 (60° C.), and vacuum dried to obtain 7 g of honeysuckle extract.

[0050] S4. Treatment of epidermal growth factor (EGF) and natural vitamin E derivatives: 10 mg EGF and 10 mg natural vitamin E derivatives were dissolved in 2 ml PBS respectively to prepare a solution with a concentration of 5 mg / ml.

[0051] S5. Preparation of the composition: take 25g of pure water, add 45g of nano-oyster shell ultrafine powder, and stir at 1200r / min for 25 minutes to form a suspension; add 8g of propolis extract, 7g of honeysuckle extract, 3g of menthol, and 3g of sodium hyaluronate to the remaining pure water, and stir at 500r / min in a 50°C water bath until dissolved and dispersed; add 2g of stabilizer (a complex of gum arabic and gelatin) and 2g of dispersant (sodium dodecylbenzene sulfonate), and stir for 45 minutes; slowly add EGF and natural vitamin E derivative solution, and stir evenly; filter and sterilize with a 0.3μm microporous filter membrane, and then fill in a spray bottle.

[0052] Comparative Example 1 (without nano-oyster shell ultrafine powder):

[0053] Preparation of propolis extract (same as Example 1);

[0054] Preparation of honeysuckle extract (same as Example 1);

[0055] Treatment of epidermal growth factor (EGF) with natural vitamin E derivatives (same as Example 1)

[0056] The composition is prepared by adding 8g propolis extract, 7g honeysuckle extract, 3g menthol, and 3g sodium hyaluronate to 25g pure water, stirring at 500r / min in a 50°C water bath until dissolved and dispersed; adding 2g stabilizer (a composite of gum arabic and gelatin) and 2g dispersant (sodium dodecylbenzene sulfonate), stirring for 45 minutes. Slowly add EGF and natural vitamin E derivative solution, stirring evenly; filtering and sterilizing with a 0.3μm microporous filter membrane, and filling in a spray bottle.

[0057] Comparative Example 2 (without propolis extract):

[0058] Preparation of nano-oyster shell ultrafine powder (same as Example 1);

[0059] Preparation of honeysuckle extract (same as Example 1);

[0060] Treatment of epidermal growth factor (EGF) with natural vitamin E derivatives (same as Example 1);

[0061] The composition is prepared by taking 25 g of pure water, adding 45 g of nano-oyster shell ultrafine powder, and stirring at 1200 r / min for 25 minutes to form a suspension; adding 7 g of honeysuckle extract, 3 g of menthol, and 3 g of sodium hyaluronate to the remaining pure water, and stirring at 500 r / min in a 50°C water bath until dissolved and dispersed; adding 2 g of stabilizer (a composite of gum arabic and gelatin) and 2 g of dispersant (sodium dodecylbenzene sulfonate), and stirring for 45 minutes; slowly adding EGF and natural vitamin E derivative solution, and stirring evenly; filtering and sterilizing with a 0.3 μm microporous filter membrane, and then filling into a spray bottle.

[0062] Comparative Example 3 (without honeysuckle extract):

[0063] Preparation of nano-oyster shell ultrafine powder (same as Example 1);

[0064] Preparation of propolis extract (same as Example 1);

[0065] Treatment of epidermal growth factor (EGF) with natural vitamin E derivatives (same as Example 1);

[0066] The composition is prepared by taking 25g of pure water, adding 45g of nano-oyster shell ultrafine powder, and stirring at 1200r / min for 25 minutes to form a suspension; adding 8g of propolis extract, 3g of menthol, and 3g of sodium hyaluronate to the remaining pure water, and stirring at 500r / min in a 50°C water bath until dissolved and dispersed; adding 2g of stabilizer (a composite of gum arabic and gelatin) and 2g of dispersant (sodium dodecylbenzene sulfonate), and stirring for 45 minutes; slowly adding EGF and natural vitamin E derivative solution, and stirring evenly; filtering and sterilizing with a 0.3μm microporous filter membrane, and then filling into a spray bottle.

[0067] Comparative Example 4 (without EGF and natural vitamin E derivatives):

[0068] Preparation of nano-oyster shell ultrafine powder (same as Example 1);

[0069] Preparation of propolis extract (same as Example 1);

[0070] Preparation of honeysuckle extract (same as Example 1);

[0071] The composition is prepared by taking 25 g of pure water, adding 45 g of nano-oyster shell ultrafine powder, and stirring at 1200 r / min for 25 minutes to form a suspension; adding 8 g of propolis extract, 7 g of honeysuckle extract, 3 g of menthol, and 3 g of sodium hyaluronate to the remaining pure water, stirring at 500 r / min in a 50°C water bath until dissolved and dispersed; adding 2 g of a stabilizer (a composite of gum arabic and gelatin) and 2 g of a dispersant (sodium dodecylbenzene sulfonate), stirring for 45 minutes; filtering and sterilizing with a 0.3 μm microporous filter membrane, and filling into a spray bottle.

[0072] The experimental tests are as follows:

[0073] Oral ulcer model establishment: healthy male or female SD rats (weight 200-250g) were selected, and a 5mm diameter round filter paper was dipped in 50% glacial acetic acid solution and applied to the buccal mucosa on the left side of the rat's mouth for 60 seconds to create an oral ulcer model.

[0074] Drug treatment: Rats were randomly divided into 8 groups, 10 in each group, including blank control group (no drug treatment, only saline spray), Example 1 group, Example 2 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group. 2 Spray the corresponding medicine evenly on the oral ulcer 3 times a day for 7 consecutive days.

[0075] Observation indicators:

[0076] Ulcer area: before medication and on the 1st, 3rd, 5th, and 7th days after medication, the long and short diameters of the oral ulcer were measured with a vernier caliper to calculate the ulcer area (ulcer area = π × long diameter × short diameter / 4);

[0077] Healing time, recording the time from the start of medication to complete healing of oral ulcers;

[0078] Histopathological observation: After 7 days of medication, the rats were killed, and the oral ulcer tissues were taken and fixed with 10% formalin, routinely paraffin-embedded, sliced, and HE-stained to observe the histopathological changes. The main observation indicators included the degree of inflammatory cell infiltration, the state of angiogenesis, and the state of epithelial tissue repair. The lower the degree of inflammatory cell infiltration, the more abundant the angiogenesis, and the more complete the epithelial tissue repair, the better the treatment effect.

[0079] Experimental results:

[0080] Ulcer area: Compared with the blank control group, the ulcer area of ​​Example 1 group was significantly reduced on the 3rd, 5th and 7th days after medication (P<0.05); the ulcer area of ​​Comparative Example 1 group (without nano-oyster shell ultrafine powder), Comparative Example 2 group (without propolis extract), Comparative Example 3 group (without honeysuckle extract) and Comparative Example 4 group (without EGF and natural vitamin E derivatives) was reduced on the 3rd, 5th and 7th days after medication, but the reduction was significantly lower than that of Example 1 group (P<0.05).

[0081] Healing time: The healing time of oral ulcers in Example 1 group was significantly shorter than that in the blank control group (P<0.05), with the average healing times being 3.5 days and 3.8 days, respectively; the healing times of Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group and Comparative Example 4 group were longer than those in Example 1 group, which were 5.2 days, 5.5 days, 5.0 days and 4.5 days, respectively (P<0.05).

[0082] Histopathological observation: After 7 days of medication, the oral ulcer tissue of Example 1 group had significantly reduced inflammatory cell infiltration, with only a small number of inflammatory cells present; abundant new blood vessels provided sufficient nutrients for tissue repair; epithelial tissue repair was relatively complete, with cells arranged neatly and the number of layers close to normal tissue. However, the inflammatory cell infiltration degree of Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 groups was relatively high, with fewer new blood vessels, poor epithelial tissue repair, disordered cell arrangement and fewer layers.

[0083] The above experimental results show that the components of the nano-oyster shell ultrafine powder composition spray of the present invention have significant synergistic effects, and the newly added EGF and natural vitamin E derivatives effectively improve the therapeutic effect, and have obvious advantages in accelerating the healing of oral ulcers. Compared with the control group without certain key ingredients, it has more outstanding performance in terms of reduced ulcer area, shortened healing time and improved tissue repair, which reflects the effectiveness and creativity of the composition spray.

[0084] The embodiments of the present invention are provided for the purpose of illustration and description. Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in this field can change, modify, replace and deform the above embodiments within the scope of the present invention.

[0085] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. "Multiple" means two or more, unless otherwise clearly and specifically defined.

[0086] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0087] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0088] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine different embodiments or examples described in this specification and the features of different embodiments or examples, unless they are contradictory.

[0089] In the drawings of the embodiments disclosed in the present invention, only the structures involved in the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0090] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A nano-oyster shell ultrafine powder composition spray, characterized in that: The invention comprises the following components: nano-sized oyster shell ultrafine powder, propolis extract, honeysuckle extract, menthol, sodium hyaluronate, stabilizer, dispersant, purified water, epidermal growth factor and natural vitamin E derivative.

2. A nano-oyster shell ultrafine powder composition spray as claimed in claim 1, characterized in that: The composition comprises, by weight: 40-50 parts of nano-oyster shell ultrafine powder, 5-10 parts of propolis extract, 5-10 parts of honeysuckle extract, 2-5 parts of menthol, 2-5 parts of sodium hyaluronate, 1-3 parts of stabilizer, 1-3 parts of dispersant, 20-30 parts of purified water, 0.5-2 parts of epidermal growth factor and 0.5-2 parts of natural vitamin E derivative.

3. A nano-oyster shell ultrafine powder composition spray as claimed in claim 2, characterized in that: The composition includes, by weight: 45 parts of the nano-oyster shell ultrafine powder, 8 parts of propolis extract, 7 parts of honeysuckle extract, 3 parts of menthol, 3 parts of sodium hyaluronate, 2 parts of stabilizer, 2 parts of dispersant, 25 parts of purified water, 1 part of epidermal growth factor, and 1 part of natural vitamin E derivative.

4. A nano-oyster shell ultrafine powder composition spray as claimed in claim 2, characterized in that: The stabilizer is a complex of sodium alginate and chitosan.

5. A nano-oyster shell ultrafine powder composition spray as claimed in claim 4, characterized in that: The dispersant is an amphiphilic polymer, the component of which is a polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer.

6. A method for preparing a nano-sized oyster shell ultrafine powder composition spray, used in conjunction with a nano-sized oyster shell ultrafine powder composition spray as described in any of claims 1 to 5, characterized in that: The method comprises the following steps: S1. Select and clean the oyster shells, and dry them in an oven at 80-100°C for 4-6 hours until they are completely dry; The dried oyster shells are crushed into coarse particles by a coarse crusher, and then crushed by a jet mill at an airflow pressure of 0.6-0.8 MPa for 30-60 minutes to obtain oyster shell ultrafine powder; The oyster shell ultrafine powder is placed in a nano grinder, 0.5% to 1% of the mass of the dispersant is added, and the powder is ground for 2 to 4 hours to obtain nano-sized oyster shell ultrafine powder with an average particle size of 50 to 200 nm; S2, freezing the propolis raw material at -5-5°C for 24-48 hours to harden and become brittle; after breaking into small pieces, adding 70%-90% edible ethanol solution at a mass ratio of 1:5-1:10, ultrasonically extracting at 200-300W ultrasonic power for 30-60 minutes in a sealed container, extracting 2-3 times; combining the extracts, removing ethanol by vacuum distillation, and drying the obtained concentrate in a vacuum drying oven at 40-60°C for 4-6 hours to obtain a propolis extract; S3, take honeysuckle medicinal material, grind it into coarse powder with a grinder; add purified water according to the corresponding mass ratio, heat and reflux to extract for 1-2 hours, and extract 2-3 times; combine the extracts, filter and concentrate under reduced pressure to an extract with a relative density of 1.1-1.3 (60°C), and then vacuum dry to obtain a honeysuckle extract; S4, dissolving the epidermal growth factor and the natural vitamin E derivative in phosphate buffer respectively to prepare a solution with a concentration of 1-5 mg / ml for later use; S5. Take an appropriate amount of pure water, slowly add the nano-oyster shell ultrafine powder, and stir at a high speed of 800-1200r / min for 20-30 minutes to form a suspension; Add propolis extract, honeysuckle extract, menthol and sodium hyaluronate to the remaining purified water in sequence, and heat and stir in a water bath (400-600r / min) until completely dissolved or evenly dispersed; Add stabilizer and dispersant, continue stirring for 30 to 60 minutes, then slowly add pre-prepared epidermal growth factor and natural vitamin E derivative solution, and stir evenly; Finally, the mixture is filtered and sterilized through a 0.22-0.45 μm microporous filter membrane, and the mixture is filled into a spray bottle to obtain a nano-oyster shell ultrafine powder composition spray.

7. A method for preparing a nano-oyster shell ultrafine powder composition spray as claimed in claim 6, characterized in that: The ratio of the honeysuckle medicinal material coarse powder to purified water is 1:8-1:

12.

8. A method for preparing a nano-oyster shell ultrafine powder composition spray as claimed in claim 7, characterized in that: The water bath temperature in S5 is 40-60°C.

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