Humic acid type microalgae biological organic fertilizer and preparation method thereof

Abstract

The invention provides a humic acid type microalgae biological organic fertilizer. The humic acid type microalgae biological organic fertilizer comprises mineral source humic acid, composite effective viable bacteria, composite microalgae seed dry powder, soybean meal and sesame cakes, the mass ratio of the mineral source humic acid to the soybean meal to the sesame-seed cake is (4.5-5): 1: 1, the invention also provides a preparation method of the fertilizer, and the preparation method at least comprises the following steps: ammoniating the mineral source humic acid, respectively grinding and crushing the soybean meal and the sesame-seed cake, and mixing with the ammoniated mineral source humic acid; then adding composite effective viable bacteria and a proper amount of water for composting fermentation until the materials are thoroughly decomposed; and adding the composite microalgae seed dry powder, uniformly stirring, granulating, drying and screening to obtain the fertilizer. The fertilizer can be applied to soybean and rice crops, promotes growth of soybean and rice root systems, helps the crops to better adapt to the environment under drought or saline-alkali conditions, further effectively improves the yield by 20-30%, can improve the fertilizer and water retention capacity of soil, can improve soil heavy metal pollution, and promotes improvement of the soil environment.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, and in particular to a humic acid type microalgae bio-organic fertilizer and a preparation method thereof. Background Art

[0002] my country is a traditional agricultural country. Fertilizer is an indispensable resource for sustainable development of mankind. Effective application of fertilizer is an important factor in increasing yield. Reasonable application of fertilizer can increase the nutrient content of soil, so that the nutrient level of soil can meet the needs of crop growth. Therefore, reasonable application of fertilizer is also an effective way to increase crop yield. However, long-term use of fertilizer may lead to environmental pollution and have a negative impact on soil and ecology. Therefore, balanced fertilization is particularly important. Balanced fertilization is a fertilization method adopted based on the relationship between the demand for nutrients during crop growth, the supply and demand of soil nutrients and the effective use of fertilizers used.

[0003] In recent years, my country's microbial fertilizers have developed rapidly, playing a positive role in protecting soil, improving ecology, increasing agricultural product output and quality, and promoting sustainable development of agricultural production. Humic acid-containing biofertilizers have the functions of promoting crop growth and development, enhancing resilience, improving product quality, and improving soil. They have been widely used in production fields such as grain crops, fruits and vegetables, forestry, and soil improvement, and have obvious effects on increasing production and efficiency of different crops. However, the incorporation of microalgae into humic acid biofertilizers and their application in grain crops still needs further development by technicians. Summary of the invention

[0004] In response to the problems in the prior art, the present invention provides a humic acid-type microalgae bio-organic fertilizer, which can be applied to soybean and rice crops to promote the growth of soybean and rice root systems, help crops better adapt to the environment under drought or saline-alkali conditions, and thereby effectively increase yields by 20-30%. At the same time, it can improve the soil's ability to retain fertilizer and water, and at the same time improve soil heavy metal pollution and promote soil environmental improvement.

[0005] To achieve the above technical purpose, the present invention provides a humic acid-type microalgae bio-organic fertilizer, including mineral humic acid, composite effective live bacteria, composite microalgae dry powder, soybean meal, and sesame cake; the mass ratio of the mineral humic acid, soybean meal, and sesame cake is (4.5-5): 1:1.

[0006] In some technical solutions of the present invention, the humic acid-type microalgae bio-organic fertilizer includes, by weight, 68-75 parts of mineral humic acid, 0.3-0.5 parts of composite effective live bacteria, 1-3 parts of composite microalgae dry powder, 13-16 parts of soybean meal, and 13-16 parts of sesame cake.

[0007] In some technical solutions of the present invention, the mineral source humic acid is selected from at least one of peat humic acid, lignite humic acid, and weathered coal humic acid.

[0008] In some technical solutions of the present invention, the mass ratio of the mineral humic acid to soybean meal and sesame cake is 70:15:15. Mineral humic acid can regulate the release efficiency of nutrients in soybean meal and sesame, avoid the rapid loss or fixation of nutrients, and make the fertilizer effect more lasting and stable. At this ratio, it can effectively promote the absorption and utilization of nutrients by the roots of soybean and rice crops, ensuring their good growth and development.

[0009] In some technical solutions of the present invention, the composite effective live bacteria are selected from at least two of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, and Paenibacillus gelatinosa.

[0010] Furthermore, the composite effective live bacteria is a composition of Bacillus subtilis and Paenibacillus jelly, which are mixed at a weight ratio of (2.8-3.2): 1. Furthermore, the specification of Bacillus subtilis is preferably 100 billion / g, and the specification of Paenibacillus jelly is preferably 10 billion / g.

[0011] In some technical solutions of the present invention, the composite microalgae dry powder includes at least two of Chlorella, Cyanobacteria and Nostoc.

[0012] Preferably, the composite microalgae dry powder includes Chlorella and Cyanobacteria, with a mass ratio of (3-4): 1, and further 3.2: 1. The combination of the two microalgae can make the biofertilizer more active, improve the nitrogen fixation effect of the soil, and further promote the growth of the roots and leaves of soybeans and rice, thereby increasing rice yield, while improving soil stability to a certain extent.

[0013] The invention also provides a method for preparing microalgae fertilizer for paddy fields: Step 1: ammoniation treatment of mineral humic acid, adding ammonium bicarbonate with a mass concentration of 9-11% of mineral humic acid and mixing evenly, heating with boiler steam to 70-80°C, and ammoniation for 70-75h; Step 2: Grind the soybean meal and sesame cake into 35-60 meshes respectively, and then mix them with the ammonified mineral humic acid; Step 3: Add compound effective live bacteria and appropriate amount of water, control the moisture content at 40-60% for compost fermentation, control the fermentation temperature at 60-65℃, and ferment for about 15-20 days until the material is mature.

[0014] Step 4: Add the composite microalgae dry powder, stir evenly, granulate, dry and sieve to obtain the humic acid type microalgae biological organic fertilizer.

[0015] The present invention also provides an application of a humic acid-type microalgae bio-organic fertilizer, and the organic fertilizer can be applied to rice and soybean planting.

[0016] The beneficial effects brought by the technical solution provided by the present invention are: 1. The humic acid-based microalgae bio-organic fertilizer provided by the present invention has an organic matter content of more than 65%, which can improve the soil aggregate structure and prevent soil compaction. The crop germination rate exceeds 99%, the yield of soybeans and rice increases by 20-30%, and the average per-acre yield of rice increases by 300kg.

[0017] 2. The mineral humic acid selected in the present invention can improve the soil structure and increase the stability of soil aggregates, further enhance the soil's ability to retain fertilizer, and at the same time provide carbon sources and energy for microorganisms. During the fermentation process, soybean meal and sesame cakes will also introduce a large number of beneficial microorganisms to form beneficial interactions with soil microorganisms, further enhancing soil activity. The mixed use of the three can achieve a balanced supply of nitrogen, phosphorus, potassium and trace elements to meet the needs of soybeans and rice at different growth stages.

[0018] 3. The combination of mineral humic acid and active bacterial agent selected by the present invention can further improve the fertilization effect, enhance the stress resistance of plants, improve the growth environment of plant roots, and can absorb heavy metals in the soil, degrade organic pollutants in the soil, supplement beneficial microorganisms in the soil, promote the soil to achieve a balance of microbial ecosystem, and alleviate soil-borne diseases. At the same time, mineral humic acid has a strong ion exchange and adsorption capacity, can absorb soil nutrients for plant absorption, microalgae can convert some insoluble nutrients into absorbable forms, and mineral humic acid can also provide trace elements required for microalgae growth. The synergistic effect of the two can better regulate the supply and release of nutrients in the soil and improve the utilization rate of fertilizers. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0020] Figure 1 This is a comparison chart of rice growth in the field experiment demonstration group (fertilization group in Example 2) and the control group (comparative example group) in this Example 2. DETAILED DESCRIPTION

[0021] The present invention will be described in detail below in conjunction with specific embodiments, but this is by no means a limitation of the present invention. The present invention will be further described below through specific embodiments.

[0022] Example 1: A humic acid-based microalgae bio-organic fertilizer, based on weight, includes 68 parts of mineral humic acid, 0.3 parts of Bacillus subtilis (specification: 100 billion / g), 0.1 parts of jelly-like Paenibacillus (specification: 10 billion / g), 2.5 parts of composite microalgae dry powder (2 parts of Chlorella and 0.5 parts of Cyanobacteria), 16 parts of soybean meal, and 16 parts of sesame cake.

[0023] The mineral humic acid is activated for 4 hours, then filtered and washed to neutral dryness for later use; the soybean meal and sesame cake are ground into 50 meshes respectively, and then mixed with the activated mineral humic acid; composite effective live bacteria and water are added, the moisture content is controlled at 55% for composting fermentation, the fermentation temperature is controlled at 60°C, and the fermentation time is 15 days, until the material is mature.

[0024] The composite microalgae dry powder is added, stirred evenly, granulated, dried and sieved to obtain the humic acid type microalgae biological organic fertilizer.

[0025] Example 2: A humic acid-based microalgae bio-organic fertilizer, calculated by weight, comprises 70 parts of mineral humic acid, 0.3 parts of Bacillus subtilis (specification: 100 billion / g), 0.1 parts of jelly-like Bacillus (specification: 10 billion / g), 2 parts of composite microalgae dry powder (2 parts of Chlorella and 0.5 parts of Cyanobacteria), 15 parts of soybean meal, and 15 parts of sesame cake.

[0026] The mineral humic acid is activated for 4 hours, then filtered and washed to neutral dryness for later use; the soybean meal and sesame cake are ground into 50 meshes respectively, and then mixed with the activated mineral humic acid; composite effective live bacteria and water are added, the moisture content is controlled at 55% for composting fermentation, the fermentation temperature is controlled at 60°C, and the fermentation time is 15 days, until the material is mature.

[0027] The composite microalgae dry powder is added, stirred evenly, granulated, dried and sieved to obtain the humic acid type microalgae biological organic fertilizer.

[0028] Example 3: A humic acid-based microalgae bio-organic fertilizer, calculated by weight, comprises 75 parts of mineral humic acid, 0.4 parts of Bacillus subtilis (specification: 100 billion / g), 0.1 parts of jelly-like Bacillus (specification: 10 billion / g), 2 parts of composite microalgae dry powder (2 parts of Chlorella and 0.5 parts of Cyanobacteria), 13 parts of soybean meal, and 13 parts of sesame cake.

[0029] The mineral humic acid is activated for 4 hours, then filtered and washed to neutral dryness for later use; the soybean meal and sesame cake are ground into 50 meshes respectively, and then mixed with the activated mineral humic acid; composite effective live bacteria and water are added, the moisture content is controlled at 55% for composting fermentation, the fermentation temperature is controlled at 60°C, and the fermentation time is 15 days, until the material is mature.

[0030] The composite microalgae dry powder is added, stirred evenly, granulated, dried and sieved to obtain the humic acid type microalgae biological organic fertilizer.

[0031] Example 4: A humic acid-based microalgae bio-organic fertilizer, calculated by weight, comprises 70 parts of mineral humic acid, 0.2 parts of Bacillus subtilis (specification: 100 billion / g), 0.1 parts of jelly-like Paenibacillus (specification: 10 billion / g), 2 parts of composite microalgae dry powder (2 parts of Chlorella and 0.5 parts of Nostoc), 15 parts of soybean meal, and 15 parts of sesame cake.

[0032] The mineral humic acid is activated for 4 hours, then filtered and washed to neutral dryness for later use; the soybean meal and sesame cake are ground into 50 meshes respectively, and then mixed with the activated mineral humic acid; composite effective live bacteria and water are added, the moisture content is controlled at 55% for composting fermentation, the fermentation temperature is controlled at 60°C, and the fermentation time is 15 days, until the material is mature.

[0033] The composite microalgae dry powder is added, stirred evenly, granulated, dried and sieved to obtain the humic acid type microalgae biological organic fertilizer.

[0034] Example 5: A humic acid-based microalgae bio-organic fertilizer, calculated by weight, comprises 75 parts of mineral humic acid, 0.3 parts of Bacillus subtilis (specification: 100 billion / g), 0.2 parts of jelly-like Bacillus (specification: 10 billion / g), 2 parts of composite microalgae dry powder (2 parts of Chlorella and 0.5 parts of Cyanobacteria), 13 parts of soybean meal, and 13 parts of sesame cake.

[0035] The mineral humic acid is activated for 4 hours, then filtered and washed to neutral dryness for later use; the soybean meal and sesame cake are ground into 50 meshes respectively, and then mixed with the activated mineral humic acid; composite effective live bacteria and water are added, the moisture content is controlled at 55% for composting fermentation, the fermentation temperature is controlled at 60°C, and the fermentation time is 15 days, until the material is mature.

[0036] The composite microalgae dry powder is added, stirred evenly, granulated, dried and sieved to obtain the humic acid type microalgae biological organic fertilizer.

[0037] Comparative Example Commercially available microbial organic fertilizer 1. For the field application of Examples 1-5 and the comparative example, 6 test fields were selected in Anhui Province to carry out the rice planting test, and the rice yield in the test fields and the yield increase compared with the commercially available biological organic fertilizer were measured.

[0038] 2. Example For soil heavy metal transfer capacity: Preparation method of adding cadmium-contaminated soil: The collected soil was air-dried and sieved, and 20 kg of soil was placed in each pot to simulate severe cadmium pollution. 65 mg of cadmium (3CdSO4•8H2O) was added to each pot, mixed evenly with the soil, numbered, and mixed evenly with the fertilizer for each fertilization treatment, and then aged for 30 days. The amount of fertilizer applied to each pot was 8.5 g. After collecting soil samples from the aged pots, deionized water was added to keep the water surface in the pot at 4 to 6 cm, and the seedlings were transplanted after 20 days of cultivation. During the experiment, water was replenished once every 2 days, and the water surface in the pot was kept at 3 to 5 cm.

[0039]

[0040] Based on the field experiments and heavy metal adsorption experiments of Examples 1-5 and the comparative example, it can be seen that the use of the humic acid-type microalgae bio-organic fertilizer provided by the present invention can achieve a maximum yield increase of 32% and reduce the cadmium accumulation of rice crops by about 25%.

[0041] Finally, a few points should be explained: Although the present invention has been described in detail above with general descriptions and specific embodiments, the above embodiments are only used to illustrate the technical solution of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that: they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents; and these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the present invention.

Claims

1. A humic acid-type microalgae bio-organic fertilizer, characterized in that: The organic fertilizer comprises mineral humic acid, composite effective live bacteria, composite microalgae dry powder, soybean meal and sesame cake; the mass ratio of the mineral humic acid, soybean meal and sesame cake is (4.5-5): 1:

1.

2. The organic fertilizer according to claim 1, characterized in that The humic acid type microalgae biological organic fertilizer comprises, by weight, 68-75 parts of mineral humic acid, 0.3-0.5 parts of composite effective live bacteria, 1-3 parts of composite microalgae dry powder, 13-16 parts of soybean meal, and 13-16 parts of sesame cake.

3. The organic fertilizer according to claim 1, characterized in that The mineral source humic acid is selected from at least one of peat humic acid, lignite humic acid and weathered coal humic acid.

4. The organic fertilizer according to claim 1, characterized in that The mass ratio of the mineral humic acid to soybean meal and sesame cake is 70:15:

15.

5. The organic fertilizer according to claim 1, characterized in that The composite effective live bacteria are selected from at least two of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Paenibacillus gelatinus.

6. The organic fertilizer according to claim 1, characterized in that The composite effective live bacteria is a composition of Bacillus subtilis and Paenibacillus jelly, which are mixed in a weight ratio of (2.8-3.2):

1.

7. The organic fertilizer according to claim 1, characterized in that The composite microalgae dry powder comprises at least two of Chlorella, Cyanobacteria and Nostoc.

8. The organic fertilizer according to claim 1, characterized in that The composite microalgae dry powder includes Chlorella and Cyanobacteria, with a mass ratio of (3-4):

1.

9. A method for preparing the humic acid-type microalgae bio-organic fertilizer according to claim 1, characterized in that: At least the following steps are included: Step 1: ammoniation treatment of mineral humic acid, adding ammonium bicarbonate with a mass concentration of 9-11% of mineral humic acid and mixing evenly, heating with boiler steam to 70-80°C, and ammoniation for 70-75h; Step 2: Grind the soybean meal and sesame cake into 35-60 meshes respectively, and then mix them with the activated mineral humic acid; Step 3: Add composite effective live bacteria and appropriate amount of water, control the moisture content at 40-60% for composting fermentation, control the fermentation temperature at 60-65°C, and ferment for about 15-20 days until the material is decomposed. Step 4: Add composite microalgae dry powder, stir evenly, granulate, dry, and sieve to obtain the humic acid type microalgae biological organic fertilizer.

10. An application of humic acid-type microalgae bio-organic fertilizer, characterized in that: The organic fertilizer can be applied to rice and soybean planting.

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