Method for preparing amino acid water-soluble fertilizer from high-concentration organic sewage
By adding wine lees extract to the vermicelli production wastewater and performing enzymatic decomposition and fermentation treatment, the problem of the inability to effectively retain amino acids and sugars in high-concentration organic wastewater is solved, and efficient resource utilization and waste reduction are achieved.
Patent Information
- Application Number
- CN202411732473.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-29
- Publication Date
- 2025-03-11
AI Technical Summary
The high concentration of organic wastewater produced during the fan production process cannot be effectively retained, resulting in waste of resources, and the existing treatment methods cannot effectively reduce the harsh impact of organic matter on the environment.
Water-soluble amino acid fertilizer is prepared by adding wine lees extract to high-concentration organic wastewater, combined with enzymatic treatment and fermentation treatment, and increasing the retention of proteins and sugars.
It effectively improves the resource utilization rate of high-concentration organic sewage, reduces waste production, increases trace elements in water-soluble fertilizers, and promotes crop growth.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of resource utilization of bean processing waste, and in particular to a method for preparing amino acid water-soluble fertilizer by utilizing high-concentration organic sewage. Background Art
[0002] During the production process of vermicelli, the raw materials need to be cleaned, soaked, refined, and formed. A large amount of wastewater will be generated in each process. For example, the wastewater in the cleaning process will carry a large amount of suspended matter, and the wastewater from cleaning vermicelli during the forming process will contain residues in the raw materials. These wastewaters mainly contain organic matter such as starch, protein, organic acid, solid suspended matter such as fiber fragments, as well as nitrogen and phosphorus. Among them, the concentration of organic matter is relatively high and will have a bad impact on the environment after being discharged into the environment. Therefore, the treatment of vermicelli wastewater is particularly important.
[0003] At present, vermicelli wastewater treatment includes screen treatment, regulating tank treatment, anaerobic treatment, aerobic treatment, etc. These treatments are used continuously to intercept large volumes of impurities first, and then stabilize the sewage quality by adjusting the sewage quality to facilitate subsequent anaerobic treatment and aerobic treatment. The above treatment can achieve harmless treatment of vermicelli wastewater to obtain recycled water, but after the above treatment, the organic matter in the vermicelli wastewater cannot be effectively retained, resulting in the problem of organic matter waste. Therefore, it is of great significance to provide a method for treating high-concentration organic wastewater to retain amino acids and sugars in organic wastewater to prepare amino acid water-soluble fertilizers and improve the resource utilization rate of high-concentration organic wastewater. Summary of the invention
[0004] In view of this, the present invention provides a method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage, specifically a method using high-concentration organic sewage as raw material, by adding wine lees extract, effectively increasing the retention of protein and sugar in the solution, so that the obtained amino acid solution can be directly used as a component of water-soluble fertilizer, and can be used after mixing with medium or trace elements. The preparation method is simple in process, easy to operate, and suitable for popularization and use; using this method, wine lees and high-concentration organic sewage are recycled and waste generation is reduced.
[0005] The technical solution of the present invention is as follows: A method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage, comprising preparing an amino acid solution and preparing a water-soluble fertilizer; The process of preparing the amino acid solution is as follows: S1, adding wine lees extract to organic sewage, stirring and standing to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 5.2±0.1, adding cellulase thereto, the amount of cellulase added is 6-6.5u / g, and performing enzymolysis at a constant temperature to obtain enzymolysis solution A; S3, filtering the enzymatic hydrolyzate A to remove solid impurities, and collecting the filtrate A; adjusting the pH of the filtrate A to 6.7±0.1, and adding magnesium sulfate, ammonium chloride, and ferrous sulfate to the filtrate A for sterilization, and then adding Bacillus subtilis to the filtrate A at an inoculum amount of 3%, and fermenting at 27±0.1°C for 36-48 hours to obtain a fermentation liquid A; filtering the fermentation liquid A, and collecting the filtrate B; S4, adjusting the pH of the filtrate B to 5.7±0.2, adding papain, bromelain and isoamylase; performing enzymolysis at a constant temperature to obtain an enzymolysis solution B, and inactivating the enzyme in the enzymolysis solution B to obtain an amino acid solution; The process of preparing water-soluble fertilizer is as follows: Add trace elements or medium elements to the amino acid solution to obtain amino acid water-soluble fertilizer.
[0006] Preferably, the wine lees extract is prepared by the following method: SS1, adding water to the wine lees, the weight ratio of the wine lees to water is 1:3-5, and beating to obtain slurry; SS2, adjusting the pH of the slurry to 4.0±0.1, then adding pectinase and cellulase to the slurry, the added amount of pectinase is 5.5-6.0u / g, the added amount of cellulase is 4.7-5.5u / g, enzymolysis is performed at 50±0.1°C for 2.5-3h, the enzyme is inactivated, and enzymolysis solution C is obtained; the enzymolysis solution C is filtered, and the filtrate C is collected; SS3, drying the filtrate C and collecting the solid; adding acetone to the solid, with the weight volume ratio of the solid to acetone (g / mL) being 1:4-6, ultrasonically extracting for 30-60 minutes, and collecting the extract; SS4, drying the extract to obtain a wine lees extract.
[0007] Preferably, before adding the wine lees extract, the protein content in the sewage is measured; the wine lees extract is added to the sewage according to the protein content; and the mass ratio of the wine lees extract to the protein is 5-7:1.
[0008] Preferably, in step S1, the stirring time is 20-30 minutes, and the standing time is 30-60 minutes to obtain a mixed solution.
[0009] Preferably, in step S2, the enzymatic hydrolysis temperature is 45° C. and the enzymatic hydrolysis time is 3-3.5 hours.
[0010] Preferably, in step S3, each liter of filtrate A contains 0.6-1.0 g of magnesium sulfate, 0.4-0.7 g of ammonium chloride, and 0.1-0.3 g of ferrous sulfate.
[0011] Preferably, in step S4, the amount of papain added is 5.2-5.5u / g, the amount of bromelain added is 3.3-3.7u / g, and the amount of isoamylase added is 6.2-6.6u / g; the enzymolysis temperature is 58±0.5°C, and the enzymolysis time is 3-5 hours.
[0012] Preferably, the secondary elements include at least one of calcium and magnesium; the trace elements include at least one of iron, manganese, zinc, copper, boron and molybdenum; and the secondary elements and trace elements are added to the amino acid solution in the form of water-soluble salts.
[0013] Compared with the prior art, the beneficial effects of the present invention are: 1. In the present invention, high-concentration organic sewage is used as raw material, and wine lees extract is used to combine with polysaccharides and proteins in the organic sewage to reduce the loss of protein and polysaccharides in subsequent treatment, so that the amino acids in the sewage can be retained in the water-soluble fertilizer as much as possible, thereby improving the utilization rate of the sewage.
[0014] 2. In the process of preparing amino acid water-soluble fertilizer, the present invention uses wine lees extract, which can not only reduce the loss of protein and polysaccharide caused by high-concentration sewage treatment, but also add trace elements to the product. The wine lees extract contains flavonoids, phenolic compounds and glycoside compounds, which can complex the elements in the water-soluble fertilizer, promote the absorption and utilization of elements by crops, and promote crop growth.
[0015] 3. The preparation method of the amino acid water-soluble fertilizer provided by the present invention effectively improves the resource utilization of high-concentration organic wastewater, and the process is simple and easy to implement. DETAILED DESCRIPTION
[0016] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.
[0017] In the present invention, the pea vermicelli wastewater used has a protein content of 8.5 g / L, a starch content of 2.45 g / L, and an organic acid content of 0.51 g / L; the mung bean vermicelli wastewater has a protein content of 5.4 g / L, a starch content of 2.2 g / L, and an organic acid content of 0.43 g / L.
[0018] In the following examples and comparative examples of the present invention, the reagents used are all commercially available products unless otherwise specified.
[0019] Example 1 A method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage, the specific steps are as follows: (1) Preparation of wine lees extract: SS1, adding water to the wine lees, the weight ratio of the wine lees to water is 1:4, and beating to obtain a slurry; the wine lees contain grape skins, seeds and part of the stalks, and beating is conducive to subsequent extraction; SS2, adjusting the pH of the slurry to 4.0, then adding pectinase and cellulase to the slurry, the added amount of pectinase is 5.7u / g, the added amount of cellulase is 5.2u / g, enzymolysis is performed at 50°C for 2.8h, the enzymes are inactivated, and enzymolysis solution C is obtained; the enzymolysis solution C is filtered and the filtrate C is collected; the enzymolysis treatment can release phenolic compounds, flavonoid compounds and glycoside compounds in the cells to the maximum extent; SS3, drying the filtrate C and collecting the solid; adding acetone to the solid, with the weight volume ratio of the solid to acetone (g / mL) being 1:5, ultrasonically extracting for 40 min, and collecting the extract; SS4, drying the extract to obtain a wine lees extract; (2) Preparation of amino acid solution: S1, taking pea vermicelli wastewater as raw material, taking 1L of pea vermicelli wastewater, adding 51g of wine lees extract thereto; the mass ratio of wine lees extract to protein is 6:1; stirring for 25 minutes, and then standing for 45 minutes to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 5.2, adding cellulase thereto, the amount of cellulase added was 6.2u / g, and performing enzymolysis at 45°C for 3.3 hours to obtain enzymolysis solution A; S3, filtering the enzymatic hydrolyzate A to remove solid impurities, and collecting the filtrate A; adjusting the pH of the filtrate A to 6.7, and adding magnesium sulfate, ammonium chloride, and ferrous sulfate to the filtrate A for sterilization, and then adding Bacillus subtilis to the filtrate A at an inoculation amount of 3%, and fermenting at 27° C. for 40 hours to obtain a fermentation liquid A; filtering the fermentation liquid A, and collecting the filtrate B; Among them, each liter of filtrate A contains 0.8g of magnesium sulfate, 0.5g of ammonium chloride, and 0.2g of ferrous sulfate; S4, adjusting the pH of the filtrate B to 5.7, adding papain, bromelain and isoamylase, wherein the amount of papain added is 5.3u / g, the amount of bromelain added is 3.5u / g, and the amount of isoamylase added is 6.4u / g; performing enzymolysis at 58°C for 4 hours to obtain enzymolysis solution B, inactivating the enzyme in the enzymolysis solution B, and obtaining an amino acid solution; (3) Preparation of amino acid water-soluble fertilizer (medium element type) liquid products The amino acid solution was concentrated to 75.2 mL by evaporation concentration, and the free amino acid content was measured to be 107.2 g / L. Then, 12.5 g of magnesium sulfate was added thereto and stirred until dissolved to obtain the product.
[0020] Comparative Example 1 The difference from Example 1 is that the process of preparing the amino acid solution is different, which is as follows: Prepare amino acid solution: S1, adjusting the pH of pea vermicelli wastewater to 5.2, adding cellulase thereto, the amount of cellulase added is 6.2u / g, and performing enzymolysis at 45°C for 3.3 hours to obtain enzymolysis solution A; S2, filtering the enzymatic hydrolyzate A to remove solid impurities, and collecting the filtrate A; adjusting the pH of the filtrate A to 6.7, and adding magnesium sulfate, ammonium chloride, and ferrous sulfate to the filtrate A for sterilization, and then adding Bacillus subtilis to the filtrate A at an inoculation amount of 3%, and fermenting at 27° C. for 40 hours to obtain a fermentation liquid A; filtering the fermentation liquid A, and collecting the filtrate B; Among them, each liter of filtrate A contains 0.8g of magnesium sulfate, 0.5g of ammonium chloride, and 0.2g of ferrous sulfate; S3, adjusting the pH of the filtrate B to 5.7, adding papain, bromelain and isoamylase, wherein the amount of papain added is 5.3u / g, the amount of bromelain added is 3.5u / g, and the amount of isoamylase added is 6.4u / g; performing enzymolysis at 58°C for 4 hours to obtain enzymolysis solution B, inactivating the enzyme in the enzymolysis solution B, and obtaining an amino acid solution.
[0021] Comparative Example 2 The difference from Example 1 is that the preparation method of the wine lees extract is different from that of Example 1. The preparation process of the wine lees extract is as follows: SS1, adding water to the wine lees, the weight ratio of the wine lees to water is 1:4, and beating to obtain a slurry; the wine lees contain grape skins, seeds and part of the stalks, and beating is conducive to subsequent extraction; SS2, adjusting the pH of the slurry to 4.0, then adding pectinase and cellulase to the slurry, the added amount of pectinase is 5.7u / g, the added amount of cellulase is 5.2u / g, enzymolysis is performed at 50°C for 2.8h, the enzyme is inactivated, and enzymolysis solution C is obtained; the enzymolysis solution C is filtered, and the filtrate C is collected; SS3, drying the filtrate C, collecting the solid, and obtaining a wine lees extract; The preparation method of the amino acid solution is the same as that in Example 1.
[0022] Comparative Example 3 The difference from Example 1 is that the process of preparing the amino acid solution is different, which is as follows: S1, taking pea vermicelli wastewater as raw material, taking 1L of pea vermicelli wastewater, adding 51g of wine lees extract thereto; the mass ratio of wine lees extract to protein is 6:1; stirring for 25 minutes, and then standing for 45 minutes to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 5.2, adding cellulase thereto, the amount of cellulase added was 6.2u / g, and performing enzymolysis at 45°C for 3.3 hours to obtain enzymolysis solution A; S3, filtering the enzymatic hydrolyzate A to remove solid impurities, and collecting the filtrate A; S4, adjusting the pH of the filtrate A to 5.7, adding papain, bromelain and isoamylase, wherein the amount of papain added is 5.3u / g, the amount of bromelain added is 3.5u / g, and the amount of isoamylase added is 6.4u / g; performing enzymolysis at 58°C for 4 hours to obtain an enzymolysis solution, inactivating the enzyme in the enzymolysis solution, and obtaining an amino acid solution.
[0023] Example 2 A method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage, the specific steps are as follows: (1) Preparation of wine lees extract: SS1, adding water to the wine lees, the weight ratio of the wine lees to water is 1:3, and beating to obtain a slurry; the wine lees contain grape skins, seeds and part of the stalks, and beating is conducive to subsequent extraction; SS2, adjusting the pH of the slurry to 3.9, then adding pectinase and cellulase to the slurry, the added amount of pectinase is 5.5u / g, the added amount of cellulase is 4.7u / g, enzymolysis is carried out at 50°C for 3h, the enzyme is inactivated, and enzymolysis solution C is obtained; the enzymolysis solution C is filtered and the filtrate C is collected; the enzymolysis treatment can release phenolic compounds, flavonoid compounds and glycoside compounds in the cells to the maximum extent; SS3, drying the filtrate C and collecting the solid; adding acetone to the solid, with the weight volume ratio of the solid to acetone (g / mL) being 1:4, ultrasonically extracting for 60 min, and collecting the extract; SS4, drying the extract to obtain a wine lees extract; (2) Preparation of amino acid solution: S1, pea vermicelli wastewater is used as raw material, 1L of pea vermicelli wastewater is taken, 42.5g of wine lees extract is added thereto; the mass ratio of wine lees extract to protein is 5:1; stirring for 20 minutes, and then standing for 30 minutes to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 5.1, adding cellulase thereto, the amount of cellulase added is 6.0 u / g, and performing enzymolysis at 45° C. for 3.5 hours to obtain enzymolysis solution A; S3, filtering the enzymatic hydrolyzate A to remove solid impurities, and collecting the filtrate A; adjusting the pH of the filtrate A to 6.6, and adding magnesium sulfate, ammonium chloride, and ferrous sulfate to the filtrate A for sterilization, and then adding Bacillus subtilis to the filtrate A at an inoculation amount of 3%, and fermenting at 27° C. for 48 hours to obtain a fermentation liquid A; filtering the fermentation liquid A, and collecting the filtrate B; Among them, each liter of filtrate A contains 0.6g of magnesium sulfate, 0.4g of ammonium chloride, and 0.1g of ferrous sulfate; S4, adjusting the pH of the filtrate B to 5.5, adding papain, bromelain and isoamylase, wherein the amount of papain added is 5.2u / g, the amount of bromelain added is 3.3u / g, and the amount of isoamylase added is 6.2u / g; performing enzymolysis at 57.5°C for 5 hours to obtain enzymolysis solution B, inactivating the enzyme in the enzymolysis solution B to obtain an amino acid solution; (3) Preparation of amino acid water-soluble fertilizer (medium element type) liquid products The amino acid solution was concentrated to 73.5 mL by evaporation concentration, and the free amino acid content was measured to be 108.1 g / L. Then, 11.6 g of magnesium sulfate was added thereto and stirred until dissolved to obtain the product.
[0024] Example 3 A method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage, the specific steps are as follows: (1) Preparation of wine lees extract: SS1, adding water to the wine lees, the weight ratio of the wine lees to water is 1:5, and beating to obtain a slurry; the wine lees contain grape skins, seeds and part of the stalks, and beating is conducive to subsequent extraction; SS2, adjusting the pH of the slurry to 4.1, then adding pectinase and cellulase to the slurry, the added amount of pectinase is 6.0u / g, the added amount of cellulase is 5.5u / g, enzymolysis is performed at 50°C for 2.5h, the enzyme is inactivated, and enzymolysis solution C is obtained; the enzymolysis solution C is filtered and the filtrate C is collected; the enzymolysis treatment can release phenolic compounds, flavonoid compounds and glycoside compounds in the cells to the maximum extent; SS3, drying the filtrate C and collecting the solid; adding acetone to the solid, with the weight volume ratio of the solid to acetone (g / mL) being 1:6, ultrasonically extracting for 30 min, and collecting the extract; SS4, drying the extract to obtain a wine lees extract; (2) Preparation of amino acid solution: S1, taking pea vermicelli wastewater as raw material, taking 1L of pea vermicelli wastewater, adding 59.5g of wine lees extract thereto; the mass ratio of wine lees extract to protein is 7:1; stirring for 30 minutes, and then standing for 60 minutes to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 5.3, adding cellulase thereto, the amount of cellulase added is 6.5u / g, and performing enzymolysis at 45°C for 3 hours to obtain enzymolysis solution A; S3, filtering the enzymatic hydrolyzate A to remove solid impurities, and collecting the filtrate A; adjusting the pH of the filtrate A to 6.8, and adding magnesium sulfate, ammonium chloride, and ferrous sulfate to the filtrate A for sterilization, and then adding Bacillus subtilis to the filtrate A at an inoculation amount of 3%, and fermenting at 27° C. for 36 hours to obtain a fermentation liquid A; filtering the fermentation liquid A, and collecting the filtrate B; Among them, each liter of filtrate A contains 1.0g of magnesium sulfate, 0.7g of ammonium chloride, and 0.3g of ferrous sulfate; S4, adjusting the pH of the filtrate B to 5.9, adding papain, bromelain and isoamylase, wherein the amount of papain added is 5.5 u / g, the amount of bromelain added is 3.7 u / g, and the amount of isoamylase added is 6.6 u / g; performing enzymolysis at 58.5° C. for 3 hours to obtain enzymolysis solution B, inactivating the enzyme in the enzymolysis solution B to obtain an amino acid solution; (3) Preparation of amino acid water-soluble fertilizer (medium element type) liquid products The amino acid solution was concentrated to 74.8 mL by evaporation concentration, and the free amino acid content was measured to be 106.7 g / L. Then, 12.2 g of magnesium sulfate was added thereto and stirred until dissolved to obtain the product.
[0025] Example 4 A method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage, the specific steps are as follows: (1) Preparation of wine lees extract: Same as Example 1; (2) Preparation of amino acid solution: S1, taking mung bean vermicelli wastewater as raw material, taking 1L of mung bean vermicelli wastewater, adding 32.4g of wine lees extract thereto; the mass ratio of wine lees extract to protein is 6:1; stirring for 25 minutes, and then standing for 45 minutes to obtain a mixed solution; S2, same as in Example 1; S3, same as in Example 1; S4, same as in Example 1; (3) Preparation of amino acid water-soluble fertilizer (trace element type) liquid products The amino acid solution was concentrated to 45.5 mL by evaporation concentration, and the free amino acid content was measured to be 109.1 g / L. Then, 3.35 g of ferric sulfate was added thereto and stirred until dissolved to obtain the product.
[0026] Detection: 1. The filtrate B obtained in Examples 1-4, the filtrate B in Comparative Example 1, the filtrate B in Comparative Example 2 and the filtrate A in Comparative Example 3 were respectively subjected to protein content detection, starch content detection and organic acid content detection. The protein detection was carried out by the biuret reagent method, the starch detection was carried out by the iodine colorimetric method, and the organic acid detection was carried out by the titration method. The test results are shown in Table 1, as follows: Table 1 Test results of protein, starch and organic acid content
[0027] It can be seen from Table 1 that after adding the wine lees extract provided by the present invention, it is possible to remove particulate impurities, suspended impurities, organic acids and other substances in organic wastewater while reducing the loss of protein and starch in the wastewater as much as possible, thereby maximizing the resource utilization of organic wastewater. 2. The free amino acid content in the amino acid solution prepared in Examples 1-4 was tested. The testing method was based on NY / T 1975 (High Performance Liquid Chromatography for the Determination of Amino Acids in Feed). The test results are shown in Table 2, as follows: Table 2 Detection results of free amino acid content in amino acid solution
[0028] 3. The amino acid water-soluble fertilizer prepared in Examples 1-4 was tested. The testing method was based on the agricultural industry standard of the People's Republic of China NY 1429-2010. The test results are shown in Tables 3 and 4, as follows: Table 3 Quality test results of amino acid water-soluble fertilizer (medium element type) of Example 1-3
[0029] Table 4 Quality test results of amino acid water-soluble fertilizer (trace element type) of Example 4
[0030] Although the present invention has been described in detail by reference to the preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, a person of ordinary skill in the art may make various equivalent modifications or substitutions to the embodiments of the present invention, and these modifications or substitutions shall be within the scope of the present invention. Any person of ordinary skill in the art may easily think of changes or substitutions within the technical scope disclosed by the present invention, and these shall be within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope of protection of the claims.
Claims
1. A method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage, characterized in that: Including preparing amino acid solution and preparing water-soluble fertilizer; The process of preparing the amino acid solution is as follows: S1, adding wine lees extract to organic sewage, stirring and standing to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 5.2±0.1, adding cellulase thereto, the amount of cellulase added is 6-6.5u / g, and performing enzymolysis at a constant temperature to obtain enzymolysis solution A; S3, filtering the enzymatic hydrolyzate A to remove solid impurities, and collecting the filtrate A; adjusting the pH of the filtrate A to 6.7±0.1, and adding magnesium sulfate, ammonium chloride, and ferrous sulfate to the filtrate A for sterilization, and then adding Bacillus subtilis to the filtrate A at an inoculum amount of 3%, and fermenting at 27±0.1°C for 36-48 hours to obtain a fermentation liquid A; filtering the fermentation liquid A, and collecting the filtrate B; S4, adjusting the pH of the filtrate B to 5.7±0.2, adding papain, bromelain and isoamylase; performing enzymolysis at a constant temperature to obtain an enzymolysis solution B, and inactivating the enzyme in the enzymolysis solution B to obtain an amino acid solution; The process of preparing water-soluble fertilizer is as follows: Add trace elements or medium elements to the amino acid solution to obtain amino acid water-soluble fertilizer.
2. The method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage as claimed in claim 1, characterized in that: The wine lees extract is prepared by the following method: SS1, adding water to the wine lees, the weight ratio of the wine lees to water is 1:3-5, and beating to obtain slurry; SS2, adjusting the pH of the slurry to 4.0±0.1, then adding pectinase and cellulase to the slurry, the added amount of pectinase is 5.5-6.0u / g, the added amount of cellulase is 4.7-5.5u / g, enzymolysis is performed at 50±0.1°C for 2.5-3h, the enzyme is inactivated, and enzymolysis solution C is obtained; the enzymolysis solution C is filtered, and the filtrate C is collected; SS3, drying the filtrate C and collecting the solid; adding acetone to the solid, with the weight volume ratio of the solid to acetone (g / mL) being 1:4-6, ultrasonically extracting for 30-60 minutes, and collecting the extract; SS4, drying the extract to obtain a wine lees extract.
3. The method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage as claimed in claim 1, characterized in that: Before adding the wine lees extract, the protein content in the sewage is measured; the wine lees extract is added to the sewage according to the protein content; the mass ratio of the wine lees extract to the protein is 5-7:
1.
4. The method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage as claimed in claim 1, characterized in that: In step S1, the stirring time is 20-30 minutes and the standing time is 30-60 minutes to obtain a mixed solution.
5. The method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage as claimed in claim 1, characterized in that: In step S2, the enzymatic hydrolysis temperature is 45° C. and the enzymatic hydrolysis time is 3-3.5 hours.
6. The method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage as claimed in claim 1, characterized in that: In step S3, each liter of filtrate A contains 0.6-1.0 g of magnesium sulfate, 0.4-0.7 g of ammonium chloride, and 0.1-0.3 g of ferrous sulfate.
7. The method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage as claimed in claim 1, characterized in that: In step S4, the amount of papain added is 5.2-5.5u / g, the amount of bromelain added is 3.3-3.7u / g, and the amount of isoamylase added is 6.2-6.6u / g; the enzymolysis temperature is 58±0.5°C, and the enzymolysis time is 3-5 hours.
8. The method for preparing amino acid water-soluble fertilizer using high-concentration organic sewage as claimed in claim 1, characterized in that: The intermediate elements include at least one of calcium and magnesium; the trace elements include at least one of iron, manganese, zinc, copper, boron and molybdenum; the intermediate elements and trace elements are added to the amino acid solution in the form of water-soluble salts.