Integrated embedding device

Abstract

The invention discloses an integrated embedding device. The integrated embedding device comprises a base and an upper shell, the upper shell comprises a shell body and a fixed film layer, the shell body is provided with a channel penetrating through a first surface and a second surface which are opposite to each other, the fixed film layer is connected to the first surface, the fixed film layer covers the channel, and the first surface and the second surface are opposite to each other. The fixing film layer is of a three-dimensional structure with pores, the three-dimensional structure is formed by interweaving a plurality of filamentous structures, the pores among at least part of the filamentous structures are communicated, and when the upper shell is matched with the base, the fixing film layer can be matched with the base to fix tissues. According to the invention, the tissue does not need to be taken out from the embedding box and transferred during embedding, so that the embedding time is saved, and splinters are not easy to occur during slicing.

Description

Technical Field

[0001] The present application relates to the technical field of biological equipment and medical devices, and in particular to an integrated embedding device. Background Art

[0002] Staining of paraffin sections of pathological tissue is an important auxiliary means for clinical diagnosis. Paraffin sections are not easy to fall off and can be kept for a long time after staining, so that paraffin sections can be sealed for a long time like patient medical records, which has a certain effect on preventing the occurrence of medical accidents, so they are widely used by major hospitals. When preparing paraffin sections, pathologists put the tissue removed from the patient into an embedding cassette, while the traditional embedding cassette is made of plastic and has multiple small holes to facilitate the circulation of reagents during dehydration. During embedding, the embedding cassette is placed in a dehydrator. After fixation, dehydration, transparency, and wax dipping, the pathologist takes the embedding cassette out of the dehydrator, takes the tissue out of the embedding cassette and places it on a stainless steel base, then uses tweezers to align and position it, adds melted paraffin through the embedding machine, and after cooling, the tissue is wrapped in paraffin to form a wax block. When the traditional embedding cassette is used for tissue embedding, it is necessary to take out the embedding cassette and transfer it to different operating platforms, which will cause a waste of time and extend the tissue processing cycle. In addition, some embedding boxes in the prior art also use a film layer to fix the tissue, but when the existing film layer is used to fix the tissue and then embed the slices, the slices are prone to splitting. Summary of the invention

[0003] Based on this, it is necessary to provide an integrated embedding device. The integrated embedding device of the present invention can realize that the tissue does not need to be taken out of the embedding box for transfer during embedding, and it is not easy to cause splitting during slicing.

[0004] An embodiment of the present application provides an integrated embedding device.

[0005] An integrated embedding device comprises a base and an upper shell, wherein the upper shell comprises a shell and a fixed film layer, wherein the shell has a channel penetrating a first surface and a second surface opposite thereto, the fixed film layer is connected to the first surface, and the fixed film layer covers the channel, wherein the fixed film layer is formed by interweaving a plurality of filamentary structures to form a three-dimensional structure having pores, wherein at least some of the pores between the filamentary structures are interconnected, and when the upper shell cooperates with the base, the fixed film layer can cooperate with the base to achieve fixation of the tissue.

[0006] In some embodiments, the upper shell also includes a supporting component, which is connected to the first surface, closer to the first surface than the fixed film layer, and at least partially extends toward the fixed film layer, and is used to support and fix the fixed film layer.

[0007] In some embodiments, the supporting component includes a plurality of supporting members, wherein at least two of the supporting members are relatively arranged on both sides of the channel, and the supporting members on both sides of the channel are respectively close to the connection between the fixed film layer and the first surface, and the supporting members are used to support the fixed film layer from the edge position of the fixed film layer.

[0008] In some embodiments, the integrated embedding device satisfies at least one of the following conditions:

[0009] (1) The support member is rotatably connected to the first surface via a rotating member, and the support member can be fixed in any rotation position;

[0010] (2) The support member is made of a deformable material;

[0011] (3) The support member is capable of elastic deformation.

[0012] In some embodiments, the supporting portion supports the film layer, the supporting film layer covers the channel, and there is a gap between the fixed film layer and the supporting film layer. When the upper shell is matched with the base, the supporting film layer is used to support and fix the fixed film layer from the middle position of the fixed film layer.

[0013] In some embodiments, the integrated embedding device satisfies at least one of the following conditions:

[0014] (1) The supporting film layer is capable of deformation;

[0015] (2) The supporting film layer is elastic;

[0016] (3) The supporting membrane layer is porous;

[0017] (4) The supporting membrane layer has an arched structure.

[0018] In some embodiments, the integrated embedding device satisfies at least one of the following conditions:

[0019] (1) The mesh size of the support film layer is 100-200 mesh;

[0020] (2) The supporting film layer is made of one or both of PET mesh and nylon.

[0021] In some embodiments, the base has a receiving groove for placing tissue. When the upper shell is matched with the base, the fixed film layer is received in the receiving groove, and the fixed film layer can cooperate with the bottom surface of the receiving groove to fix the tissue.

[0022] In some embodiments, the supporting film layer and the fixed film layer are respectively arched structures with the same bending direction, and the maximum height of the fixed film layer arched relative to the first surface is equal to the depth of the receiving groove, or the maximum height of the fixed film layer arched relative to the first surface is 1mm~10mm greater than the depth of the receiving groove.

[0023] In some embodiments, two opposite sides of the supporting film layer and / or the fixed film layer are respectively connected to a first side edge and a second side edge of the channel.

[0024] In some embodiments, the maximum gap between the fixed film layer and the supporting film layer is 1 mm to 2 mm.

[0025] In some embodiments, the integrated embedding device satisfies at least one of the following conditions:

[0026] (1) The shell also has a plurality of through holes penetrating the first surface and the second surface;

[0027] (2) A plurality of fins are provided on the edge of the top surface of the base, wherein two opposite fins extend toward the top of the base, and two opposite fins extend toward the bottom of the base;

[0028] (3) A limiting groove is provided on the top surface of the base, the bottom surface of the limiting groove is provided with the accommodating groove, and the first surface of the shell can be in contact with and cooperate with the bottom surface of the limiting groove;

[0029] (4) The fixed membrane layer has an arched structure;

[0030] (5) The fixed film layer is a hot-melt mesh film;

[0031] (6) The width of the fixed film layer is 1 cm to 10 cm.

[0032] In some of the embodiments, the fixed membrane layer has a multi-layer filamentous structure in the thickness direction, each layer has pores, and the pores on each layer are interconnected.

[0033] In some embodiments, the pores of the fixed membrane layer are interconnected.

[0034] Compared with the conventional technology, the above-mentioned integrated embedding device has at least the following beneficial effects:

[0035] (1) The present application is not prone to splitting during slicing. During the embedding process, since the tissue is positioned by the fixed membrane layer, the fixed membrane layer has multiple three-dimensional structures with pores formed by filamentary structures. The fixed membrane layer has irregular pore shapes in both the two-dimensional plane structure and the three-dimensional structure. Since the three-dimensional structure is formed by the interweaving of several filamentary structures, the pores between the filamentary structures are interconnected and can be immersed in molten paraffin. The paraffin can be immersed in the pores of the fixed membrane layer. After the paraffin is cooled, the solidified wax block is continuous rather than discontinuous. Therefore, basically no splitting will occur when the wax block is sliced.

[0036] (2) The present application can greatly save labor and embedding time. The present application sets a base and an upper shell to cooperate with each other, and the fixed film layer can cooperate with the base, and the fixed film layer can cooperate with the base to fix the tissue. The fixed film layer is then supported by a supporting component including a supporting film layer or a supporting member to prevent the fixed film layer from loosening. The supporting film layer and / or the supporting member support the fixed film layer. In this way, the supporting film layer and / or the supporting member cooperate with the fixed film layer to fix the tissue and keep the tissue in position. In the embedding process, after the above-mentioned integrated embedding device with fixed tissue is placed in a dehydrator for dehydration, the embedding box is taken out of the dehydrator, and melted paraffin can be directly added through the embedding machine. During this period, there is no need to take out the tissue from the embedding box, transfer it, and reposition it, which greatly saves labor and embedding time. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application, and those skilled in the art can obtain other drawings based on these drawings without creative work.

[0038] In order to more completely understand the present application and its beneficial effects, the following description will be given in conjunction with the accompanying drawings. In the following description, the same reference numerals represent the same parts.

[0039] Figure 1 A schematic diagram of a base of an integrated embedding device according to an embodiment of the present invention;

[0040] Figure 2 A schematic diagram of an upper shell of an integrated embedding device according to an embodiment of the present invention;

[0041] Figure 3 A schematic diagram of an upper shell of an integrated embedding device according to another embodiment of the present invention;

[0042] Figure 4 A schematic diagram of an upper shell of an integrated embedding device according to another embodiment of the present invention;

[0043] Figure 5 It is a schematic side view of the upper shell of the integrated embedding device according to one embodiment of the present invention;

[0044] Figure 6 A schematic diagram of the base and tissue placement of the integrated embedding device according to an embodiment of the present invention;

[0045] Figure 7 It is a side schematic diagram of the integrated embedding device and tissue embedding according to one embodiment of the present invention;

[0046] Figure 8 This is a schematic diagram of the thickness of the wax block that can be sliced ​​according to an embodiment of the present invention;

[0047] Fig. 9 This is a physical diagram of the upper shell described in one embodiment of the present invention.

[0048] Description of Reference Numerals

[0049] 10. Integrated embedding device; 100. Base; 110. Top surface; 120. Accommodating groove; 130. Limiting groove; 140. Fin; 200. Upper shell; 210. Shell; 211. First surface; 212. Second surface; 213. Channel; 214. Support member; 215. Through hole; 216. Rotating member; 220. Supporting film layer; 230. Fixed film layer; 20. Tissue. DETAILED DESCRIPTION

[0050] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0051] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0052] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0053] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0054] In the description of the present invention, "several" means more than one, "many" means more than two, "greater than", "less than", "exceed", etc. are understood to exclude the number itself, and "above", "below", "within", etc. are understood to include the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0055] In this article, "optionally", "optional", "optional" means optional, that is, it means to be selected from any of the two parallel solutions of "yes" or "no". If there are multiple "optional" in a technical solution, unless otherwise specified and there is no contradiction or mutual restriction, each "optional" is independent. In this application, the descriptions such as "optionally contain" and "optionally include" mean "contain or not contain".

[0056] In the present application, when it comes to a numerical interval (i.e., a numerical range), unless otherwise specified, the distribution of the optional numerical values ​​in the numerical interval is considered to be continuous, and includes the two numerical endpoints (i.e., the minimum and maximum values) of the numerical interval, and each numerical value between the two numerical endpoints. Unless otherwise specified, when the numerical interval only refers to an integer in the numerical interval, it includes the two endpoint integers of the numerical range, and each integer between the two endpoints, which is equivalent to directly listing each integer. When multiple numerical ranges are provided to describe features or characteristics, these numerical ranges can be combined. In other words, unless otherwise specified, the numerical range disclosed in the present application should be understood to include any and all sub-ranges included therein. The "numerical value" in the numerical interval can be any quantitative value, such as a number, a percentage, a ratio, etc. "Numerical interval" allows for a broad range of quantitative intervals such as percentage intervals, ratio intervals, and ratio intervals.

[0057] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which the present invention belongs. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0058] The embodiment of the present application provides an integrated embedding device to solve the problem that there is a time interval between each step in the process of preparing paraffin sections in the prior art, which causes a waste of time and prolongs the cycle of tissue 20 processing; in the traditional process of preparing paraffin sections, after the dehydrator is processed, the pathologist needs to replace the embedding box one by one and add paraffin to the tissue 20 one by one, which affects the stability of the quality of the wax block; and the film layer used in the embedding box of the prior art is easy to cause splitting when slicing after fixing the tissue. The integrated embedding device will be described below in conjunction with the accompanying drawings.

[0059] The integrated embedding device 10 provided in the embodiment of the present application is exemplary, see Figure 1 and Figure 2 As shown, Figure 1 This is a schematic structural diagram of a base 100 of an integrated embedding device 10 provided in an embodiment of the present application. Figure 2 Schematic diagram of the upper shell 200 of the integrated embedding device 10 of one embodiment of the present invention. The integrated embedding device 10 of the present application can be used for paraffin sectioning, and can save manpower, improve efficiency and reduce costs during paraffin sectioning. In order to more clearly illustrate the structure of the integrated embedding device 10, the integrated embedding device 10 will be introduced in conjunction with the accompanying drawings.

[0060] For example, see Figure 1 , Figure 2As shown, an integrated embedding device 10 includes a base 100 and an upper shell 200. The upper shell 200 includes a shell 210 and a fixed film layer 230. The shell 210 has a channel 213 that penetrates the first surface 211 and the second surface 212 that are opposite to each other. The channel 213 enables the embedded tissue 20 to communicate with the outside. The fixed film layer 230 is connected to the first surface 211. The fixed film layer 230 covers the channel 213. It should be noted that the covering here does not mean that the fixed film layer 230 completely closes the channel 213, but covers the opening of the channel 213 above the channel 213. In the present application, when the upper shell 200 cooperates with the base 100, the fixed film layer 230 can cooperate with the base 100 to fix the tissue 20, wherein the fixed film layer 230 is used to fix the tissue 20.

[0061] In some embodiments, the fixed film layer 230 is formed by interweaving a plurality of filamentary structures to form a three-dimensional structure with pores. It should be noted that the number of pores in the fixed film layer 230 is multiple, and the pores are gaps between a plurality of filamentary structures after the filamentary structures are interwoven, and the gaps can be penetrated by molten paraffin. When the molten paraffin penetrates the pores and solidifies, a substantially continuous embedding surface can be formed on the surface of the tissue 20, wherein the portion where the tissue 20 contacts the filamentary structures of the fixed film layer 230 is a discontinuous surface.

[0062] In some of these embodiments, at least some of the pores between the filamentous structures are interconnected.

[0063] Preferably, all pores between the filamentous structures are interconnected.

[0064] In some embodiments, the fixed film layer 230 has a multi-layer filament structure in the thickness direction, and each layer has pores. Fig. 9 As shown, Fig. 9 It is a real picture of the upper shell 200, wherein the fixed film layer 230 is formed into a multi-layer flocculent by a plurality of filamentous structures randomly distributed.

[0065] In some embodiments, the pores of each layer of the fixed membrane layer 230 are interconnected.

[0066] In some embodiments, the pores of each layer of the fixed membrane layer 230 in the thickness direction are interconnected. Preferably, the pores of the fixed membrane layer 230 are all interconnected.

[0067] In some embodiments, all pores in the fixed film layer 230 are interconnected. It should be noted that the fixed film layer 230 is in the shape of a film as a whole, and the interior of the film is a three-dimensional structure. In the three-dimensional structure, multiple filamentary structures are randomly distributed and interwoven, and pores are formed between the multiple filamentary structures. Therefore, there are meshes on any two-dimensional cross-section of the three-dimensional structure. The pores can be immersed in paraffin, and since the pores are interconnected, the paraffin can basically enter all the pores to form a continuous wax block.

[0068] The present application sets the base 100 and the upper shell 200 to cooperate with each other, and the fixed film layer 230 can cooperate with the base 100, and the fixed film layer 230 can cooperate with the base 100 to achieve the fixation of the tissue 20. In the embedding process, after the above-mentioned integrated embedding device 10 with the tissue 20 fixed is placed in a dehydrator for dehydration, the embedding box is taken out of the dehydrator, and the melted paraffin can be directly added through the embedding machine, and there is no need to take out the tissue 20 from the embedding box for transfer and repositioning, which greatly saves labor and embedding time. In the embedding process, the fixed film layer 230 has a plurality of meshes formed by a filamentous structure, and these meshes are all interconnected and can be immersed in molten paraffin. After the paraffin is cooled, the solidified wax block is continuous rather than discontinuous, so basically no cracks will be generated when the wax block is sliced.

[0069] In some cases, the fixed membrane may float due to the buoyancy of tissue fluid and / or molten paraffin, resulting in the fixed membrane being unable to fix the sample tissue. Based on this, in some embodiments, the upper shell 200 also includes a supporting component, which is connected to the first surface 211. The supporting component is closer to the first surface 211 than the fixed membrane layer 230, and the supporting component at least partially extends toward the fixed membrane layer 230. The supporting component is used to support and fix the fixed membrane layer 230.

[0070] In some embodiments, the supporting component includes a supporting member 214, and the number of the supporting members 214 is multiple, wherein at least two supporting members 214 are relatively arranged on both sides of the channel 213, and the supporting members 214 on both sides of the channel 213 are respectively close to the connection between the fixed film layer 230 and the first surface 211, and the supporting members 214 are used to support the fixed film layer 230 from the edge position of the fixed film layer 230.

[0071] Preferably, one side of the support member 214 is connected to the first surface 211 at an edge of the channel 213 , and the other side of the support member 214 opposite thereto extends toward the fixed film layer 230 .

[0072] In some embodiments, the support member 214 may be in a columnar, block-shaped, plate-shaped, etc. The support member 214 only needs to be able to support the fixed film layer 230 .

[0073] In some of these examples, see Figure 2 , Figure 3 As shown, the support member 214 is a curved structure. Preferably, the curvature of the support member 214 is adapted to the curvature of the fixed film layer 230 in the reset state.

[0074] In some of these examples, see Figure 2 , Figure 3 As shown, the length of the support member 214 extending toward the fixed film layer 230 can be adjusted as needed.

[0075] In some of these examples, see Figure 3 As shown, the support member 214 and the first surface 211 are rotatably connected via the rotating member 216 , and the support member 214 can be fixed at any rotation position.

[0076] In some of the embodiments, the rotating member 216 may be an articulated structure such as a hinge or a hinge.

[0077] In some embodiments, the angle between the support member 214 and the fixed film layer 230 can be adjusted as needed. For example, when the fixed film layer 230 is in the reset state, the support member 214 and the fixed film layer 230 are in contact with each other, or when the fixed film layer 230 is in the reset state, there is a certain gap between the support member 214 and the fixed film layer 230. Figure 2 , Figure 3 As shown, Figure 2 , Figure 3 The support member 214 and the fixed film layer 230 are shown to be in a contacting and mating relationship.

[0078] Preferably, in a specific example, there are two support members 214, which are relatively arranged on both sides of the channel 213, and both support members 214 are curved structures, and the curvature of the support members 214 is substantially the same as the curvature of the fixed film layer 230 in the reset state.

[0079] In some embodiments, the support member 214 is made of a deformable material, such as plastic, silicone, rubber, etc.

[0080] In some of these embodiments, the support member 214 is capable of elastic deformation.

[0081] More preferably, see Figure 4 As shown, the opening of the channel 213 is a rectangular opening, and the two side edges of the channel 213 in the length direction form a first side edge and a second side edge respectively. The support member 214 is connected to the first side edge and the second side edge.

[0082] In some of these examples, see Figure 4 , Figure 5 As shown, the support component also includes a support film layer 220. The support film layer 220 and the fixed film layer 230 are respectively connected to the first surface 211. The fixed film layer 230 is located outside the support film layer 220, that is, the support film layer 220 is closer to the first surface 211 than the fixed film layer 230. The support film layer 220 covers the channel 213. In the present application, when melted paraffin is added to the base 100 by an embedding machine, since the material of the fixed film layer 230 is very light, the fixed film layer 230 may float due to the buoyancy of the molten paraffin. At this time, the fixed film layer 230 cannot maintain contact with the tissue 20 and fix the tissue 20 well. Therefore, the present application supports the fixed film layer 230 from the middle position of the fixed film layer 230 by setting the support film layer 220 to prevent the fixed film layer 230 from floating and keep the fixed film layer 230 in contact with the tissue 20 to improve the embedding effect.

[0083] In some of these examples, see Figure 4 , Figure 5 As shown, the support component may include a support member 214 alone or include a support film layer 220 .

[0084] In some embodiments, the support component includes both the support member 214 and the support film layer 220. At this time, the two side edges of the support film layer 220 are connected to the support member 214 at the two side positions of the channel 213 respectively.

[0085] In some of the embodiments, the support film layer 220 is capable of deforming.

[0086] In some embodiments, the supporting film layer 220 is elastic. The supporting film layer 220 has elastic properties and can undergo a certain degree of deformation, so it can support the fixing film layer 230 and prevent the fixing film layer 230 and the tissue 20 from loosening.

[0087] In some embodiments, the support film layer 220 is porous. The support film layer 220 is porous and has the following advantages: (1) Lightweight: The porous structure usually means that the material contains a large number of voids, so the overall density is low and the weight is light. (2) Good permeability: The pores of the support film layer 220 allow tissue fluid and / or molten paraffin to pass through.

[0088] In some embodiments, the supporting film layer 220 has an arched structure, which can effectively support the fixed film layer 230 .

[0089] Preferably, in a specific example, the supporting film layer 220 has a porous arched structure.

[0090] In some embodiments, the fixed film layer 230 has an arched structure.

[0091] Preferably, the curvature of the supporting film layer 220 is the same as the curvature of the fixed film layer 230 .

[0092] In some embodiments, the base 100 has a receiving groove 120. When in use, the shell 210 is covered on the base 100 and closes the notch of the receiving groove 120, and the channel 213 enables the receiving groove 120 to communicate with the outside. When the upper shell 200 is matched with the base 100, the supporting film layer 220 and the fixing film layer 230 are both accommodated in the receiving groove 120, and the fixing film layer 230 can cooperate with the bottom surface of the receiving groove 120 to fix the tissue 20.

[0093] In some embodiments, the top surface of the base 100 has a receiving groove 120 .

[0094] In some of these embodiments, see Figure 4 As shown, the shell 210 is a flat block structure as a whole, and two opposite surfaces of the shell 210 form a first surface 211 and a second surface 212 respectively.

[0095] In some embodiments, there is a gap between the non-end position of the fixed film layer 230 and the non-end position of the supporting film layer 220. That is, the end of the fixed film layer 230 and the end of the supporting film layer 220 are respectively connected to the first surface. Figure 4 As shown, the two ends of the fixed film layer 230 correspond to and fit with the two ends of the supporting film layer 220 .

[0096] In some embodiments, the mesh number of the support film layer 220 is 100 mesh to 200 mesh. For example, the mesh number of the support film layer 220 is 100 mesh, 110 mesh, 130 mesh, 150 mesh, 170 mesh, 180 mesh, 200 mesh or other values.

[0097] In some embodiments, the supporting film layer 220 is made of one or both of PET mesh and nylon. The supporting film layer 220 with a mesh size of 100 to 200 can support and fix the film layer 230 on the one hand, and facilitate the flow of reagents on the other hand. The PET film layer has the following advantages: (1) High mechanical strength: The PET film layer has high tensile strength and good toughness. Even when it is thin, it can maintain good mechanical properties and is suitable for applications requiring wear resistance and tear resistance. (2) Good transparency: The PET film layer usually has high transparency and a light transmittance of more than 90%. It is suitable for applications requiring high transparency such as packaging materials and display screen protective films. (3) Thermal stability: The PET film layer maintains stable physical properties over a wide temperature range and can withstand high temperatures without significant deformation. It is suitable for processing that requires heat treatment. (4) Chemical stability: The PET film layer has good resistance to most solvents, acids, alkalis and other chemicals, is not easily corroded, and is suitable for environments in contact with chemicals. (5) Environmentally friendly and recyclable: PET is a recyclable material that can be reused after being discarded, reducing environmental pollution and meeting the requirements of sustainable development. (6) Cost-effectiveness: Compared with other high-performance plastic film layers, PET film layers have lower costs and higher cost-effectiveness, making them suitable for large-scale production and application. (7) Easy to process: PET film layers can be made into products of different shapes and thicknesses through a variety of processing technologies (such as blow molding, extrusion, coating, etc.), and have strong adaptability.

[0098] Nylon material has many excellent physical and chemical properties, including (1) high strength: Nylon fibers are very strong and have good tensile strength. (2) wear resistance: Nylon has good resistance to wear and tear, so it is often used to manufacture products that require durability. (3) elasticity: Nylon fibers have certain elasticity and recovery, and can withstand a certain degree of deformation and then return to their original shape. Therefore, the support film layer 220 of nylon material can improve the support strength of the fixed film layer 230 and improve the positioning effect of the support and tissue 20. (4) chemical resistance: Nylon has good resistance to most chemicals, but strong acids and strong alkalis may cause damage to it. (5) hygroscopicity: Nylon can absorb a certain amount of moisture and can absorb excess moisture during the slicing process. It is not difficult to understand that in other embodiments, the preparation material of the support film layer 220 can also be other types of materials.

[0099] Preferably, the supporting film layer 220 is made of PET mesh.

[0100] In some embodiments, the supporting film layer 220 and the fixed film layer 230 are respectively in an arched structure with the same bending direction. Figure 5 As shown, the supporting film layer 220 and the fixing film layer 230 are both arched and bent in a direction away from the housing 210 .

[0101] In some embodiments, the maximum height of the fixed film layer 230 protruding relative to the first surface 211 is equal to the depth of the receiving groove 120 .

[0102] In some embodiments, the maximum height of the fixing film layer 230 arched relative to the first surface 211 is greater than or equal to the depth of the receiving groove 120. The above configuration is mainly to ensure that the fixing film layer 230 and the bottom surface of the receiving groove 120 can clamp and fix the tissue 20.

[0103] In some embodiments, the maximum height of the fixed film layer 230 relative to the first surface 211 is 1 mm to 10 mm greater than the depth of the receiving groove 120. The maximum height of the fixed film layer 230 relative to the first surface 211 is determined according to the size of the tissue 20 and the depth of the receiving groove 120.

[0104] In some embodiments, the width of the fixed film layer 230 is 1 cm to 10 cm. The fixed film layer 230 has a certain width to ensure the thickness of the wax block. For example, the width of the fixed film layer 230 is 1 cm, 2 cm, 3 cm, 5 cm, 8 cm, 9 cm, 10 cm or other values. It should be noted that the length of the fixed film layer 230 refers to the total length between the two ends of the fixed film layer 230 connected to the first surface 211, and the width of the fixed film layer 230 refers to the direction perpendicular to the line connecting the two ends of the fixed film layer 230 connecting the first surface 211 ( Figure 4 The width in the X direction as shown.

[0105] In some embodiments, two opposite sides of the supporting film layer 220 are respectively connected to a first side edge and a second side edge of the channel 213 .

[0106] In some of these embodiments, see Figure 5 As shown, there is a gap between a portion of the fixed film layer 230 and the supporting film layer 220 .

[0107] In some of these embodiments, see Figure 5 As shown, Figure 5The upper shell 200 is a side view of the integrated embedding device 10 of an embodiment of the present invention. The maximum gap between the fixed film layer 230 and the supporting film layer 220 is 1mm~2mm. There is a gap between the supporting film layer 220 and the fixed film layer 230. The gap between the supporting film layer 220 and the fixed film layer 230 should not be too large or too small. When the gap is too small, the slices are likely to cut the supporting film layer 220 and cause cracks; when the gap is too large, the supporting film layer 220 cannot support the fixed film layer 230 well, causing the fixed film layer 230 to deform. In this application, after multiple experimental tests, for tissues 20 with a thickness of less than 2mm, the gap between the supporting film layer 220 and the fixed film layer 230 is 1mm, which is the most suitable. It should be noted that, since both ends of the fixed film layer 230 and the supporting film layer 220 are connected to the first surface, the maximum gap between the fixed film layer 230 and the supporting film layer 220 refers to the distance between the middle position of the arched fixed film layer 230 and the middle position of the arched supporting film layer 220. In addition, the thickness of the tissue 20 refers to Figure 8 The distance between the upper and lower arrows in the angle shown.

[0108] In some embodiments, the fixing film layer 230 is a hot-melt mesh film, which is light and easy to deform, and can keep the tissue 20 in position without causing a split effect when the wax block is sliced.

[0109] In some embodiments, the hot-melt web is formed by spinneret preparation.

[0110] In some of the embodiments, the hot melt mesh includes a plurality of filamentary structures, which are interwoven to form a three-dimensional structure. In the three-dimensional structure, there are multiple pores, so that the hot melt mesh has mesh holes in any two-dimensional cross-section and three-dimensional structure.

[0111] It should be noted that hot melt mesh is a special form of hot melt adhesive product, usually in the form of a mesh structure, similar in appearance to non-woven fabrics. Hot melt mesh is different from ordinary mesh in that it is spun and meshed using specialized equipment, so that the hot melt mesh as a whole has multiple meshes formed by interweaving filamentary structures, making the hot melt mesh very light and suitable for positioning tissues. It should be noted that the traditional ordinary mesh is a regular, coherent structure with roughly "well"-shaped holes, and the solid part of the mesh is tight, so it cannot be immersed in paraffin to form a continuous state. When ordinary mesh is used for slicing, the paraffin will be separated into multiple independent units after filling, resulting in discontinuous paraffin. The two-dimensional cross-section of the ordinary mesh and the paraffin on the three-dimensional structure are not coherent, which will cause subsequent slicing to split. Based on this, in the present application, the hot-melt mesh formed by spin-weaving is a three-dimensional structure in which filamentary structures are randomly interwoven. Therefore, the mesh distribution of the hot-melt mesh on a single plane two-dimensional cross-section is messy and disordered. Therefore, after paraffin filling, it can be immersed in the pores between multiple filamentary structures. Since the pores formed by the filamentary structures are basically interconnected, a continuous wax block is formed after the paraffin solidifies, and no splitting will occur in subsequent slicing.

[0112] In some of the embodiments, the hot melt web can be purchased from Suzhou Huiyang Adhesive Products Co., Ltd., model: W6165TDS.

[0113] In some of these embodiments, see Figure 4 As shown, the housing 210 also has a plurality of through holes 215 penetrating the first surface 211 and the second surface 212. The housing 210 is provided with a plurality of through holes 215 to facilitate the flow of reagents.

[0114] In some of these embodiments, see Figure 4 As shown, the multiple through holes 215 on the housing 210 are distributed on both sides of the channel 213. It is not difficult to understand that in one specific example, the multiple through holes 215 on the housing 210 can also be distributed on the four sides of the channel 213. In the present application, a plurality of through holes 215 are provided on the housing 210, which can realize the circulation of reagents between the first surface 211 and the second surface 212 on the housing 210, facilitate the slicing operation, and also reduce the frequent manual disassembly and assembly between the housing 210 and the base 100.

[0115] In some of these embodiments, please refer again to Figure 1As shown, the top surface 110 of the base 100 is provided with a limiting groove 130. The bottom surface of the limiting groove 130 is provided with a receiving groove 120. During assembly, the first surface 211 of the housing 210 can be in contact with the bottom surface of the limiting groove 130, and the support portion 214 extends into the receiving groove 120, and the supporting film layer 220 and the fixed film layer 230 are completely located in the receiving groove 120. Such an arrangement can improve the compactness of the assembly and improve the slicing effect.

[0116] In some embodiments, a plurality of fins 140 are disposed on the edge of the top surface 110 of the base 100. Two of the fins 140 that are opposite to each other extend toward the top of the base 100. When the base 100 is provided with the receiving groove 120, the limiting groove 130 and the plurality of fins 140, see Figure 1 As shown, the base 100 is a box-shaped structure as a whole. The present application sets a plurality of fins on the edge of the top surface 110 of the base 100, and the fins 140 at different positions can improve the stability of the base 100, prevent the base 100 from tipping over during the slicing process, and improve the slicing stability.

[0117] In some embodiments, the base 100 is made of stainless steel. Since there is a certain difference in the shrinkage rate between stainless steel and paraffin, the base 100 made of stainless steel can facilitate the separation of the tissue 20 from the wax block and the fixing film layer 230 after embedding, thereby improving the slicing efficiency.

[0118] The above-mentioned integrated embedding device 10, when in use, specifically comprises the following steps:

[0119] (1) See Figure 6 As shown, Figure 6 1 is a schematic diagram of the placement of the base 100 and the tissue 20 of the integrated embedding device 10 according to an embodiment of the present invention. The tissue 20 is placed in the middle position of the bottom surface of the receiving groove 120 of the base 100 .

[0120] (2) The upper shell 200 is turned upside down, the fixed film layer 230 covers and closes the notch of the receiving groove 120, and the fixed film layer 230 is brought into contact with the tissue 20, and the fixed film layer 230 fixes the tissue 20, see Figure 7 As shown, Figure 7This is a side view of an integrated embedding device 10 and a tissue 20 embedded in an embodiment of the present invention. The fixed film layer 230 is deformed by the restriction of the tissue 20. The deformed fixed film layer 230 positions the tissue 20. Since there is a gap between the supporting film layer 220 and the fixed film layer 230, there is no contact between the supporting film layer 220 and the tissue 20, or the supporting member 214 contacts and cooperates with the edge of the fixed film layer 230 and has no contact with the tissue 20. The upper shell 200 and the base 100 are assembled and fixed, and the fixed state is maintained throughout the paraffin embedding process. After the integrated embedding device 10 with the tissue 20 fixed is placed in a dehydrator for dehydration, the embedding box is taken out of the dehydrator.

[0121] (3) The embedding machine injects melted paraffin into the receiving groove 120 of the base 100 through the through hole 215, and the supporting film layer 220 supports the fixed film layer 230, and / or the supporting member 214 supports the edge position of the fixed film layer 230. The fixed film layer 230 maintains contact with the tissue 20. After the paraffin cools down, the tissue 20 is embedded.

[0122] (4) The upper shell 200 is removed, and the fixed film layer 230 gradually separates from the base 100 as the upper shell 200 moves. The paraffin, the tissue 20 and part of the fixed film layer 230 form an embedded wax block. Since the solidified paraffin is continuous rather than discontinuous in the mesh of the fixed film layer 230, when the embedded wax block is sliced, the obtained slices only contain paraffin, part of the filamentous fixed film layer 230 and the tissue 20, without other soft materials. Therefore, the situation of slice splitting can be avoided, and the obtained slices will basically not have splits. Figure 8 As shown, Figure 8 This is a schematic diagram of the thickness of a wax block that can be sliced ​​according to an embodiment of the present invention. Figure 8 The ranges of the two arrows in the middle indicate the thickness of the wax block that can be sliced.

[0123] In summary, the present application is not prone to splitting during slicing. In the embedding process, since the tissue 20 is positioned by the fixed film layer 230, the fixed film layer 230 has a plurality of three-dimensional structures with pores formed by filamentary structures, and the fixed film layer 230 has irregular pore shapes in both the two-dimensional plane structure and the three-dimensional structure. Since the three-dimensional structure is formed by the interweaving of a plurality of filamentary structures, the pores between the filamentary structures are interconnected, and the pores can be immersed in molten paraffin, and the paraffin can be immersed in each gap of the fixed film layer 230. After the paraffin is cooled, the solidified wax block is continuous rather than discontinuous, so basically no splitting will occur when the wax block is sliced.

[0124] The integrated embedding device 10 of the present application can greatly save labor and embedding time. In the present application, a base 100 and an upper shell 200 are arranged to cooperate with each other, and the fixed film layer 230 can cooperate with the base 100, and the fixed film layer 230 can cooperate with the base 100 to fix the tissue 20. Since there is a certain gap between the supporting film layer 220 and the fixed film layer 230, in the initial stage of contacting the tissue 20, the fixed film layer 230 first contacts the tissue 20 and fixes the tissue 20, and then the supporting film layer 220 supports the fixed film layer 230 to prevent the fixed film layer 230 from loosening, and the supporting film layer 220 supports the fixed film layer 230. In this way, the supporting film layer 220 and the fixed film layer 230 cooperate with each other to fix the tissue 20 and keep the tissue 20 in position; and / or, the fixed film layer 230 is supported by the supporting member 214 from the edge position of the fixed film layer 230, and the supporting member 214 provides a certain supporting force to the fixed film layer 230 to keep the fixed film layer 230 fixed and positioned on the tissue 20. In the embedding process, after the integrated embedding device 10 with the tissue 20 fixed thereon is placed in a dehydrator for dehydration, the embedding box is taken out of the dehydrator and melted paraffin can be directly added through the embedding machine. During this period, there is no need to take out, transfer and reposition the tissue 20 from the embedding box, which greatly saves labor and embedding time.

[0125] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0126] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0127] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An integrated embedding device, characterized in that: It includes a base and an upper shell, the upper shell includes a shell and a fixed film layer, the shell has a channel running through its first surface and second surface which are opposite to each other, the fixed film layer is connected to the first surface, the fixed film layer covers the channel, the fixed film layer is formed by interweaving a plurality of filamentous structures to form a three-dimensional structure with pores, at least some of the pores are connected, and when the upper shell cooperates with the base, the fixed film layer can cooperate with the base to fix the tissue.

2. The integrated embedding device according to claim 1, characterized in that: The upper shell further comprises a supporting component, the supporting component is connected to the first surface, the supporting component is closer to the first surface than the fixed film layer, the supporting component at least partially extends toward the fixed film layer, and the supporting component is used to support and fix the fixed film layer.

3. The integrated embedding device according to claim 2, characterized in that: The supporting component includes a plurality of supporting members, wherein at least two of the supporting members are relatively arranged on two sides of the channel, and the supporting members on both sides of the channel are respectively close to the connection between the fixed film layer and the first surface, and the supporting members are used to support the fixed film layer from the edge position of the fixed film layer.

4. The integrated embedding device according to claim 3, characterized in that: The integrated embedding device meets at least one of the following conditions: (1) The support member is rotatably connected to the first surface via a rotating member, and the support member can be fixed in any rotation position; (2) The support member is made of a deformable material; (3) The support member is capable of elastic deformation.

5. The integrated embedding device according to claim 2, characterized in that: The supporting component includes a supporting film layer, which covers the channel. There is a gap between the fixed film layer and the supporting film layer. When the upper shell is matched with the base, the supporting film layer is used to support and fix the fixed film layer from the middle position of the fixed film layer.

6. The integrated embedding device according to claim 5, characterized in that: The integrated embedding device meets at least one of the following conditions: (1) The supporting film layer is capable of deformation; (2) The supporting film layer is elastic; (3) The supporting membrane layer is porous; (4) The supporting membrane layer has an arched structure; (5) The mesh size of the support film layer is 100-200 mesh; (6) The supporting film layer is made of one or both of PET mesh and nylon.

7. The integrated embedding device according to any one of claims 5 to 6, characterized in that: The base has a receiving groove for placing tissues. When the upper shell cooperates with the base, the fixing film layer is received in the receiving groove, and the fixing film layer can cooperate with the bottom surface of the receiving groove to fix the tissues.

8. The integrated embedding device according to claim 7, characterized in that: The supporting film layer and the fixed film layer are respectively arched structures with the same bending direction, and the maximum height of the fixed film layer arched relative to the first surface is equal to the depth of the accommodating groove, or the maximum height of the fixed film layer arched relative to the first surface is 1mm~10mm greater than the depth of the accommodating groove.

9. The integrated embedding device according to any one of claims 5 to 6 and 8, characterized in that: The integrated embedding device meets at least one of the following conditions: (1) The two opposite sides of the supporting membrane layer and / or the fixed membrane layer are respectively connected to the first side edge and the second side edge of the channel; (2) The maximum gap between the fixed film layer and the supporting film layer is 1 mm to 2 mm.

10. The integrated embedding device according to any one of claims 1 to 5 and 7, characterized in that: The integrated embedding device meets at least one of the following conditions: (1) The shell also has a plurality of through holes penetrating the first surface and the second surface; (2) A plurality of fins are provided on the edge of the top surface of the base, wherein two opposite fins extend toward the top of the base, and two opposite fins extend toward the bottom of the base; (3) The fixed membrane layer has an arched structure; (4) The fixed film layer is a hot-melt mesh film; (5) The width of the fixed film layer is 1 cm to 10 cm; (6) The fixed membrane layer has a multi-layer filamentous structure in the thickness direction, each layer has pores, and the pores on each layer are interconnected; (7) The pores of the fixed membrane layer are all interconnected.

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