Blood spot sample collection method and blood spot perforating machine

Abstract

The invention relates to the technical field of blood spot sample collection, in particular to a blood spot sample collection method and a blood spot perforating machine, the method comprises the following steps: S1, placing a blood sample collection card at a set position; s2, acquiring a surface image of the blood sample collection card, analyzing the quality of blood spots on the blood sample collection card, and calibrating the blood spots; s3, punching the blood sample collection card according to a calibration result in the step S2 to obtain a blood spot sample; and S4, placing the blood spot sample in a blood spot collector, obtaining image information in the blood spot collector, analyzing and determining whether the blood spot falls into a corresponding hole site through the image information, if so, continuing punching and sampling, and if not, re-sampling. The detection efficiency can be greatly improved, and false detection is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of blood smear sample collection, and in particular to a blood smear sample collection method and a blood smear puncher. Background Art

[0002] The blood smear sample is collected through a blood sample collection card. The blood sample collection card is usually composed of filter paper and a blood sample. There are multiple blood spots infiltrated on the filter paper. A puncher is used to punch holes in the filter paper at the blood spot position to form a blood smear, and the components in the blood spot are analyzed through the blood smear.

[0003] The punching machines in the prior art include manual punching machines and automatic punching machines. The manual punching machine punches holes manually, and after collecting the blood smear, the blood smear is manually placed in the corresponding hole position of the blood smear collector. This punching method is suitable for collecting a small amount of blood smears and does not require the commissioning of large equipment, but the punching efficiency is low, the punching accuracy is insufficient, and the use is limited.

[0004] An automatic puncher uses automated means to punch holes with high efficiency and precision. However, due to the uneven distribution of blood spots on the blood sample collection card, the quality of the collected blood smears varies, and some blood smears cannot even be tested, resulting in sampling failure and affecting detection efficiency. Summary of the invention

[0005] In order to solve the above technical problems existing in the prior art, the present invention provides a blood smear sample collection method and a blood smear punching machine.

[0006] To achieve the above object, the technical solution of the present invention is as follows:

[0007] In one aspect, the present invention provides a method for collecting a blood sample, comprising:

[0008] S1. Place the blood sample collection card in the set position;

[0009] S2, obtaining a surface image of the blood sample collection card, analyzing the quality of the blood spots on the blood sample collection card, and calibrating the blood spots;

[0010] S3, according to the calibration result in step S2, punching the blood sample collection card to obtain a blood sample;

[0011] S4. Place the blood smear sample in a blood smear collector, obtain image information in the blood smear collector, and confirm whether the blood smear falls into the corresponding hole position through image information analysis. If so, continue to punch holes for sampling; if not, re-sample.

[0012] Furthermore, in step S1, the blood sample collection card is placed on a rotating device, and during the punching process, the rotating device drives the blood sample collection card to rotate.

[0013] Furthermore, in step S2, the surface image of the blood sample collection card is obtained, specifically, the images of the upper surface and the lower surface of the blood sample collection card are obtained.

[0014] Furthermore, analyzing the quality of the blood spot on the blood sample collection card in step S2 includes: analyzing the size, concentration, and blood volume of the blood spot on the blood sample collection card.

[0015] Furthermore, before punching the blood sample collection card in step S3, the surface of the punching blade is subjected to a destaticizing treatment.

[0016] Furthermore, the anti-static treatment is specifically as follows: before punching, the punching blade is blown with air, and the humidity of the air used for blowing is ≥ 60%.

[0017] Furthermore, the upper limit of the humidity of the gas used for blowing is determined according to the ambient temperature. The ambient temperature is set to T, and the maximum humidity that ensures no condensation at temperature T is the upper limit of the gas humidity.

[0018] Furthermore, in step S4, image information in the blood flake collector is obtained, and whether the blood flake falls into the corresponding hole position is confirmed by analyzing the image information, specifically including: obtaining a top-down image containing the hole position information in the blood flake collector, comparing the top-down image with the top-down image of the hole position in the blood flake collector without a blood flake placed therein, determining the position of the hole position into which the blood flake falls, and then determining whether the blood flake falls into the corresponding hole position.

[0019] On the other hand, the present invention also provides a blood smear puncher, which adopts the above-mentioned blood smear sample collection method, including a puncher body, an air blowing system, an image acquisition system and a control system, wherein the air blowing system and the image acquisition system are both connected to the control system; the air blowing system includes a nozzle, and the nozzle is arranged toward the punching head of the puncher body.

[0020] Furthermore, the image acquisition system includes an image acquisition device, and the image acquisition device is a camera.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The blood spot sample collection method and blood spot punching machine provided by the present invention collect the surface image of the blood sample collection card before punching, analyze the blood spot quality, calibrate the blood spot, and then perform the punching process. If the blood spot quality is qualified, punching is performed. If the blood spot quality is unqualified, blood spots at other positions are collected to avoid the collected blood spots not meeting the detection requirements. The present invention can greatly improve the detection efficiency and avoid false detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The present invention provides a flow chart of the blood smear sample collection method.

[0024] Figure 2 This is a schematic structural diagram of the blood film punching machine provided by the present invention.

[0025] Description of reference numerals:

[0026] 1- bracket, 2- automatic punching device, 3- blowing system, 301- air chamber, 302- air pipe. DETAILED DESCRIPTION

[0027] The technical solution of the present invention will be clearly described below in conjunction with the accompanying drawings. Obviously, the described embodiments are not all embodiments of the present invention, and all other embodiments obtained by ordinary technicians in the field without making creative work are within the protection scope of the present invention.

[0028] It should be noted that, unless otherwise specifically stated, the relative arrangements of components and steps, and numerical expressions set forth in these embodiments should not be construed as limiting the scope of the present invention.

[0029] The following description of the exemplary embodiments is merely illustrative and is not intended to limit the present invention and its application or use in any sense. Techniques, methods and devices known to ordinary technicians in the relevant field may not be discussed in detail here, but where applicable, these techniques, methods and devices should be considered as part of this specification.

[0030] Embodiment 1

[0031] This embodiment provides a blood sample collection method, such as Figure 1 As shown, including:

[0032] S1. Place the blood sample collection card in the set position;

[0033] S2, obtaining a surface image of the blood sample collection card, analyzing the quality of the blood spots on the blood sample collection card, and calibrating the blood spots;

[0034] S3, according to the calibration result in step S2, punching the blood sample collection card to obtain a blood sample;

[0035] S4. Place the blood smear sample in a blood smear collector, obtain image information in the blood smear collector, and confirm whether the blood smear falls into the corresponding hole position through image information analysis. If so, continue to punch holes for sampling; if not, re-sample.

[0036] Blood spots are distributed on the blood sample collection card, and certain data acquisition is achieved by detecting information such as the components in the blood spots. The blood sample collection card is placed in a set position, usually a fixed position on a puncher, such as a turntable. Then, an image acquisition system is used to obtain a surface image of the blood sample collection card, which has an image of the surface state of the blood spots, and then the quality of the blood spots is analyzed according to the image analysis software. The image analysis software can be a machine learning module, which first obtains a certain amount of blood spot images to calibrate the blood spot quality, and then divides the blood spot images into a training set and a test set, and inputs the training set into the machine learning model for model training. After the model accuracy reaches the threshold, it is tested using a test machine.

[0037] After the analysis, the blood spots in the image are calibrated to determine which positions have qualified blood spots and which positions have unqualified blood spots, and then the blood spots in the qualified positions are collected. The collection method is to punch holes in the blood spot positions to form blood flakes. The blood flakes are then placed in a blood flake collector. Since the blood flakes are light in weight, the blood flakes may not fall into the corresponding holes during the falling process, resulting in the subsequent blood spot positions not corresponding to the blood flakes. Therefore, after collecting the blood flakes, the image information in the blood flake collector is obtained, and the image information is analyzed to confirm whether the blood flakes fall into the corresponding holes. If so, continue to punch holes for sampling, otherwise resample. The image analysis to confirm whether the blood flakes fall into the corresponding holes can be performed by a before-and-after comparison method, that is, the surface images of the blood flake collector with or without blood flakes are stored in advance, and after a blood flake falls, the surface images of the blood flake collector are recollected, and the before-and-after images are compared to determine the falling position of the blood flakes. The previous image of each subsequent comparison is the image collected after the last blood flake fell.

[0038] Among them, in step S1, the blood sample collection card is placed on the rotating device. During the punching process, the rotating device drives the blood sample collection card to rotate. The rotation of the blood sample collection card can promote the punching head to quickly cut the blood sample collection card.

[0039] Preferably, since some blood sample collection cards use filter paper, after the blood penetrates the filter paper, the penetration conditions of the upper surface and the lower surface are different. Therefore, it is necessary to analyze the upper and lower surface images of the blood sample collection card. Therefore, in step S2, the surface image of the blood sample collection card is obtained, preferably: the images of the upper surface and the lower surface of the blood sample collection card are obtained.

[0040] Analyzing the quality of the blood spot on the blood sample collection card in step S2 includes: analyzing the size, concentration, and blood volume of the blood spot on the blood sample collection card.

[0041] In addition, before punching the blood sample collection card in step S3, the surface of the punching head is subjected to anti-static treatment. Specifically, before punching, the punching head is blown with air, and the humidity of the gas used for blowing is ≥ 60%. The upper limit of the humidity of the gas used for blowing is determined according to the ambient temperature, and the ambient temperature is set to T. The maximum humidity that does not condense at temperature T is the upper limit of the gas humidity. After the punching head is blown with gas with high humidity, water molecules are present on the surface of the punching head, which improves the conductivity of the punching head, thereby preventing the generation and accumulation of static electricity. Static electricity will not occur during the punching process, avoiding the deviation of the blood film due to static electricity problems, and improving the punching efficiency and quality.

[0042] In step S4, image information in the blood flake collector is obtained, and whether the blood flake falls into the corresponding hole position is confirmed by analyzing the image information, which specifically includes: obtaining a top-down picture containing hole position information in the blood flake collector, comparing the top-down picture with the top-down picture of the hole position in the blood flake collector without a blood flake placed therein, determining the hole position where the blood flake falls therein, and then determining whether the blood flake falls therein. After the first blood flake falls into the hole position, the top-down picture to be compared is the top-down picture of the hole position in the blood flake collector without a blood flake placed therein and the top-down picture of the hole position in the blood flake collector after the blood flake is placed therein. After the second blood flake falls into the hole position, the top-down picture to be compared is the top-down picture of the hole position in the blood flake collector with the first blood flake placed therein and the top-down picture of the hole position in the blood flake collector after the second blood flake is placed therein, and so on.

[0043] Embodiment 2

[0044] This embodiment provides a blood smear puncher, which adopts the blood smear sample collection method provided in the first embodiment. Figure 2 As shown, it includes a punching machine body, an air blowing system 3, an image acquisition system and a control system, wherein the air blowing system and the image acquisition system are both connected to the control system; the air blowing system includes a nozzle, and the nozzle is arranged toward the punching head of the punching machine body. The image acquisition system includes an image acquisition device, and the image acquisition device is a camera.

[0045] The punching machine body is an existing punching machine device, including a bracket 1, an automatic punching device 2 and a control system. The blowing system 3 includes an air bin 301, an air pipe 302, and a temperature and humidity sensor. The air bin 301 is arranged above the bracket 1, one end of the air pipe 302 is connected to the air bin 301, and a nozzle is arranged at the other end of the air pipe 302, the nozzle is arranged toward the punching cutter head, and the temperature and humidity sensor is arranged in the air bin 301. The control system is connected to the temperature and humidity sensor.

[0046] The temperature and humidity in the air bin 301 are detected by using a temperature and humidity sensor, and the humidity range is determined by using the temperature. The humidity in the air bin 301 can be adjusted by inputting normal temperature water vapor or normal temperature air into the air bin 301.

[0047] The image acquisition system, control system and punching machine body are all existing equipment, and their specific structures are not described in detail again.

[0048] The above specific implementation methods are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to examples, a person skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the scope of the technical solution of the present invention, which should be included in the scope of the claims of the present invention.

Claims

1. A blood sample collection method, characterized in that: include: S1. Place the blood sample collection card in the set position; S2, obtaining a surface image of the blood sample collection card, analyzing the quality of the blood spots on the blood sample collection card, and calibrating the blood spots; S3, according to the calibration result in step S2, punching the blood sample collection card to obtain a blood sample; S4. Place the blood smear sample in a blood smear collector, obtain image information in the blood smear collector, and confirm whether the blood smear falls into the corresponding hole position through image information analysis. If so, continue to punch holes for sampling; if not, re-sample.

2. The blood sample collection method according to claim 1, characterized in that: In step S1, the blood sample collection card is placed on a rotating device. During the punching process, the rotating device drives the blood sample collection card to rotate.

3. The blood sample collection method according to claim 1, characterized in that: In step S2, the surface image of the blood sample collection card is obtained, specifically: images of the upper surface and the lower surface of the blood sample collection card are obtained.

4. The blood sample collection method according to claim 3, characterized in that: Analyzing the quality of the blood spot on the blood sample collection card in step S2 includes: analyzing the size, concentration, and blood volume of the blood spot on the blood sample collection card.

5. The blood sample collection method according to claim 1, characterized in that: Before punching the blood sample collection card in step S3, the surface of the punching blade is subjected to a destaticizing treatment.

6. The blood sample collection method according to claim 5, characterized in that: The anti-static treatment is specifically as follows: before punching, the punching cutter head is blown with air, and the humidity of the gas used for blowing is ≥ 60%.

7. The blood sample collection method according to claim 6, characterized in that: The upper limit of the humidity of the gas used for blowing is determined according to the ambient temperature. The ambient temperature is set to T. The maximum humidity that ensures no condensation at temperature T is the upper limit of the gas humidity.

8. The blood sample collection method according to claim 1, characterized in that: In step S4, image information in the blood flake collector is obtained, and whether the blood flake falls into the corresponding hole position is confirmed by analyzing the image information, which specifically includes: obtaining a top-down image containing hole position information in the blood flake collector, comparing the top-down image with a top-down image of the hole position in the blood flake collector without a blood flake placed therein, determining the position of the hole position into which the blood flake falls, and then determining whether the blood flake falls into the corresponding hole position.

9. A blood smear puncher, using the blood smear sample collection method according to any one of claims 1 to 8, characterized in that: It includes a punching machine body, an air blowing system, an image acquisition system and a control system, wherein the air blowing system and the image acquisition system are both connected to the control system; the air blowing system includes a nozzle, and the nozzle is arranged toward the punching cutter head of the punching machine body.

10. The blood film punching machine according to claim 9, characterized in that: The image acquisition system comprises an image acquisition device, and the image acquisition device is a camera.

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