Experimental device for copying ischemic animal model and use method of experimental device

Abstract

The invention relates to the technical field of ischemic animal models, in particular to an experimental device for copying an ischemic animal model and a using method thereof.The experimental device comprises a base, a fixing mechanism and an adjusting mechanism, a touch display screen is arranged on the base, a fixing frame of the fixing mechanism is connected with the top of the base, and the fixing frame is hinged to an adjusting screw of the adjusting mechanism; the movable base is connected with a piston rod of the injector, the injector is fixedly connected with a fixing groove of the fixing frame, the end, away from the fixing groove, of the fixing frame is connected with the movable motor, the injector is communicated with the three-way valve through the pressure measuring connecting pipe, the three-way valve is communicated with the blood pressure transducer, and the blood pressure transducer is electrically connected with the biological signal collecting and processing system. A wireless Bluetooth assembly of the biological signal collecting and processing system transmits signals to the control system. By adopting the structure, the copying precision of the model and the reliability of the experimental result are improved, the operation process is simplified, the personal error is reduced, various experimental requirements such as arterial blood pressure regulation and urine generation are met, and the experimental efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of ischemic animal models, and in particular to an experimental device for replicating an ischemic animal model and a use method thereof. Background Art

[0002] In the functional experiment course, the replication of the ischemic experimental model is an extremely important teaching link, which integrates the application of multiple disciplines to cultivate students' ability to use comprehensive knowledge to analyze and solve problems. The traditional method often uses a 50mL syringe or a glass bottle with a syringe-like shape, filled with a certain amount of 200U / mL heparin saline as a bloodletting bottle, and fixes it at a certain height to make an ischemic model and a tool for adjusting blood pressure. Due to the obvious differences in blood volume between individual animals, as the amount of bloodletting gradually increases, the difference in the increase in the liquid level of the bloodletting bottle also gradually increases, resulting in large differences in experimental results and insufficient adjustment accuracy, which seriously affects the accuracy and reliability of the experimental results.

[0003] In addition, although the height of the bloodletting bottle is positively correlated with blood pressure, it is not proportional; adjusting the blood pressure by adjusting the height of the bloodletting bottle can only play a rough adjustment role, and cannot accurately adjust the blood pressure. Especially in experiments to replicate hemorrhagic shock, a slight over-adjustment can lead to experimental failure. In summary, traditional ischemic experimental model replication and blood pressure regulation methods have significant technical limitations, and a new device and method that can improve experimental accuracy, model replication success rate and blood pressure regulation accuracy is urgently needed. Summary of the invention

[0004] The purpose of the present invention is to provide an experimental device for replicating an ischemic animal model and a method for using the same, aiming to improve the replication accuracy and experimental efficiency of the ischemic experimental model.

[0005] To achieve the above-mentioned purpose, the present invention provides an experimental device for replicating an ischemic animal model, comprising a base, a fixing mechanism and an adjusting mechanism. A touch display screen is provided on the base. A fixing frame of the fixing mechanism is connected to the top of the base. The fixing frame is hinged to an adjusting screw of the adjusting mechanism. A piston adapter on a movable seat is connected to a piston rod of a syringe. The syringe is fixedly connected to a fixing groove of the fixing frame. One end of the fixing frame away from the fixing groove is connected to a movable motor. The syringe is connected to a three-way valve through a pressure measuring connecting tube. The three-way valve is connected to a blood pressure transducer. The blood pressure transducer is electrically connected to a biological signal acquisition and processing system.

[0006] Preferably, the mobile motor is connected to the adjusting screw, and limit rods are symmetrically provided on both sides of the adjusting screw. The limit rods are slidably connected to the mobile seat, and both ends of the limit rods are connected to the fixed frame. Both ends of the fixed frame are provided with limit switches on the outside of the limit rods.

[0007] Preferably, the fixing groove on the fixing frame matches the shape of the bottom of the syringe, the locking frame at the rear end of the fixing groove is connected to the rear of the syringe, and both sides of the locking frame are threadedly connected to the fixing frame through locking spring screws.

[0008] Preferably, the touch display screen, limit switch and moving motor are all electrically connected to the control system, and the wireless Bluetooth component of the biological signal acquisition and processing system transmits signals to the control system.

[0009] The method for using the above-mentioned experimental device for replicating an ischemic animal model comprises the following steps when automatically replicating a blood pressure model: S1. Use a syringe to draw 200 U / mL heparin saline, expel the air in the syringe and the pressure measuring connecting tube connected to it, fix the syringe on the fixing frame through the locking frame, and connect the pressure measuring connecting tube on the syringe to the three-way valve; The other two interface channels of the three-way valve in S2 and S1 are connected to the blood pressure transducer and the animal artery, respectively, and the syringe extraction or infusion speed, syringe specifications, and animal weight are set through the touch screen; S3. Setting the number of models for the ischemic animal experiment through the touch screen, and inputting the expected blood pressure value corresponding to each model, setting the blood pressure constant time through the touch screen, and the control system comparing the actual blood pressure value with the expected blood pressure value, and controlling the mobile motor to drive the syringe to work in real time according to the comparison result; S4. After each model reaches the expected blood pressure value and maintains the blood pressure constant for a certain period of time, it enters the next model and records the compensatory blood volume of the previous model until all models are completed.

[0010] Preferably, in S3, when the actual blood pressure value is greater than the expected blood pressure value, the mobile motor drives the syringe to draw blood until the expected blood pressure value is reached and the blood pressure is kept constant.

[0011] Preferably, in S3, when the actual blood pressure value is less than the expected blood pressure value, the mobile motor drives the syringe to perform infusion until the blood pressure reaches the expected blood pressure value and the blood pressure is kept constant.

[0012] The method for using the above-mentioned experimental device for replicating an ischemic animal model includes the following steps when performing automatic volume pumping and filling: T1. Use a syringe to draw out the experimental drug solution and expel the air in the syringe and the pressure measuring connecting tube connected to it. Fix the syringe on the fixing frame through the locking frame and connect the pressure measuring connecting tube on the syringe to the artery or vein of the experimental animal. T2. Set the syringe extraction or infusion speed, syringe specifications and animal weight through the touch screen; T3. Set the number of models through the touch screen, input the expected blood volume for each model, and set the holding time after reaching the expected blood volume through the touch screen; T4, the control system controls the mobile motor to drive the syringe to move according to the expected blood volume in T3, and enters the holding time after reaching the expected blood volume. After reaching the holding time, it enters the next model until all models are completed.

[0013] Preferably, in T3, the operation for the expected blood volume is to start the moving motor to drive the syringe to draw blood during extraction.

[0014] Preferably, in T3, the operation for the expected blood volume is to start the moving motor to drive the syringe to perfuse the drug solution for the experiment during perfusion.

[0015] Therefore, the present invention adopts an experimental device for replicating an ischemic animal model with the above structure and a method of using the same, and its beneficial effects are: 1. The device and method of use provided by the present invention improve the replication accuracy of the experimental model and the reliability of the experimental results, simplify the operation process, reduce human errors, are suitable for various experimental needs such as arterial blood pressure regulation and urine production, and improve experimental efficiency; 2. The device and method of use provided by the present invention can monitor the compensatory capacity of body fluids, and in the case of blood loss, test the state in which the body's compensatory capacity is the strongest, and the rate of urine production under different blood pressure states; 3. The device provided by the present invention can be used in two ways: automatic blood pressure replication and automatic volume pumping and filling. It can display the blood loss or blood pressure status of animals in real time and can set the index of compensatory capacity (compensatory ability); 4. The device provided by the present invention can replace the infusion pump to work.

[0016] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of an experimental device for replicating an ischemic animal model according to the present invention (the blood pressure transducer and the three-way valve are not shown).

[0018] Reference numerals: 1. Base; 11. Touch screen; 2. Fixing mechanism; 21. Fixing frame; 22. Locking frame; 23. Fixing slot; 3. Adjusting mechanism; 31. Moving motor; 32. Adjusting screw; 33. Limiting rod; 34. Moving seat; 4. Syringe; 5. Limit switch. DETAILED DESCRIPTION

[0019] The present invention is further described below in conjunction with the accompanying drawings and embodiments. Unless otherwise defined, the technical terms or scientific terms used in the present invention should be the common meanings understood by people with ordinary skills in the field to which the present invention belongs. The above-mentioned features or features mentioned in the specific examples mentioned in the present invention can be combined arbitrarily, and these specific embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0020] Example 1 like Figure 1 As shown, the present invention provides an experimental device for replicating an ischemic animal model, comprising a base 1, a fixing mechanism 2 and an adjusting mechanism 3. A touch screen 11 is provided on the base 1, a fixing frame 21 of the fixing mechanism 2 is connected to the top of the base 1, the fixing frame 21 is hinged to the adjusting screw 32 of the adjusting mechanism 3, and a moving seat 34 threadedly connected to the adjusting screw 32 is connected to the piston rod of the syringe 4. The syringe 4 is fixedly connected to the fixing groove of the fixing frame 21, and one end of the fixing frame 21 away from the fixing groove is connected to the moving motor 31. The syringe 4 is connected to the three-way valve through a pressure measuring connecting pipe, and the three-way valve is connected to the blood pressure transducer.

[0021] The moving motor 31 is connected to the adjusting screw 32, and limit rods 33 are symmetrically provided on both sides of the adjusting screw 32. The limit rods 33 are slidably connected to the moving seat 34. Both ends of the limit rods 33 are connected to the fixing frame 21, and both ends of the fixing frame 21 are provided with limit switches 5 on the outside of the limit rods 33.

[0022] The blood pressure transducer is connected to the artery of the experimental animal through a pressure measuring connecting tube, a three-way valve, a pressure measuring connecting tube, and an arterial cannula. The other channel of the three-way valve is connected to the syringe of the experimental device through a pressure measuring connecting tube. The signal interface of the blood pressure transducer is electrically connected to the input signal interface of the biological signal acquisition and processing system. The biological signal acquisition and processing system software of the computer in the biological signal acquisition and processing system displays the actual blood pressure value, and transmits the actual blood pressure value to the control system through a wireless Bluetooth component. According to the expected blood pressure value and the actual blood pressure value, the control system drives the regulating mechanism to automatically adjust the amount of bloodletting or injection to achieve precise control of the blood pressure value. The biological signal acquisition and processing system is a means in the prior art.

[0023] The fixing mechanism 2 fixes the adjusting mechanism 3 and the syringe 4, the adjusting mechanism 3 controls the movement of the syringe 4, and the blood pressure transducer monitors the blood pressure of the animal in real time. The moving motor 31 is started, and the adjusting screw 32 rotates and drives the moving seat 34 to move forward and backward, and the moving seat 34 drives the piston rod to move forward and backward. The setting of the limit rod 33 ensures the stability of the moving seat 34 during the movement. The setting of the limit switch 5 limits the movement range of the moving seat 34.

[0024] The fixing groove 23 on the fixing frame 21 matches the shape of the syringe 4, and the locking frame 22 at the rear end of the fixing groove 23 is connected to the rear of the syringe 4. The locking frame 22 is threadedly connected to the fixing frame 21 through a spring screw. The locking frame 22 fixes the syringe 4 between the fixing groove 23 and the locking frame 22. The distance between the locking frame 22 and the fixing groove 23 can be adjusted through the spring screw, which is convenient for fixing syringes 4 of different specifications. The specifications of the syringe 4 are one of 100mL, 50mL, 20mL or 10mL, which is selected according to the experimental requirements.

[0025] The touch screen 11, the limit switch 5 and the moving motor 31 are all electrically connected to the control system, the output interface of the blood pressure transducer is electrically connected to the signal input port of the biological signal acquisition and processing system, and the wireless Bluetooth component of the biological signal acquisition and processing system wirelessly transmits the blood pressure measured value signal to the control system by means in the prior art. By means in the prior art, the blood pressure value, total blood loss, animal ischemia degree and other indicators monitored in real time by the biological signal acquisition and processing system software are displayed on the touch screen 11.

[0026] Example 2 The method for using the experimental device for replicating an ischemic animal model in Example 1 includes the following steps when automatically replicating blood pressure: S1. Use a syringe to draw out 200U / mL heparin saline to expel the air in the syringe and the pressure measuring connecting tube connected to it, fix the syringe on the fixing frame through the locking frame, and connect the pressure measuring connecting tube on the syringe to the three-way valve.

[0027] The other two channels of the three-way valve in S2 and S1 are connected to the blood pressure transducer and the artery of the experimental animal respectively through the pressure measuring connecting tube. The extraction or infusion speed of the syringe is set through the touch screen. The speed of extracting or infusing the solution is recommended to be less than 20ml / min.

[0028] Animal Type: Select Rabbit or Rat.

[0029] Animal weight: Weigh the rabbit or rat before the experiment and enter the actual weight (unit: Kg) of the rabbit or rat weighed before the experiment on the touch screen.

[0030] Syringe size: Choose from 100mL, 50mL, 20mL or 10mL syringes.

[0031] S3. Set the number of models for ischemic animal experiments through the touch screen, and enter the expected blood pressure value for each model. Set the constant blood pressure time through the touch screen, press the "Start" button to start the mobile motor, and receive the measured blood pressure value recorded by the biological signal acquisition and processing system (response time <= 300 milliseconds / time). The control system compares the measured blood pressure value with the expected blood pressure value, and controls the mobile motor to drive the syringe to work in real time according to the comparison result.

[0032] In S3, when the actual blood pressure value is greater than the expected blood pressure value, the mobile motor drives the syringe to draw blood until the expected blood pressure value is reached. The mobile motor stops rotating, and the rabbit or rat undergoes body compensation (tissue fluid penetrates and flows into the blood vessels), causing the blood pressure to gradually increase. When the actual blood pressure value is greater than the expected blood pressure value, the mobile motor starts again to draw the compensatory blood volume into the syringe, keeping the blood pressure constant at the expected blood pressure value during the blood pressure constant time.

[0033] In S3, when the actual blood pressure value is lower than the expected blood pressure value due to continuous blood loss or other reasons, the mobile motor drives the syringe to infuse until the expected blood pressure value is reached to keep the blood pressure constant.

[0034] S4. After each model reaches the expected blood pressure value and maintains the blood pressure constant for a period of time, it enters the next model and records the compensatory blood volume of the previous model until all models are completed.

[0035] Using the above steps, the models set are model A, model B, model C, model D and model E in order. Each model can set the expected blood pressure value and blood pressure constant time according to the experimental requirements. Each model was set to drop by 10 mmHg, with a constant blood pressure time of 5 minutes. Rabbits were 2.5 kg in weight and had a 100 mL syringe. The injection rate was 10 mL / min. The blood pressure of normal rabbits was 95.2 mmHg (mean arterial pressure, obtained by adding 1 / 3 of the systolic pressure to 2 / 3 of the diastolic pressure). The results are shown in Table 1.

[0036] Table 1. Data tables of various models

[0037] Example 3 The method for using the experimental device for replicating an ischemic animal model in Example 1 includes the following steps when performing automatic volume pumping and filling: T1. Use a syringe to draw out the experimental drug solution (such as saline) to expel the air in the syringe and the pressure measuring connecting tube connected to it, fix the syringe on the fixing frame through the locking frame, and connect the pressure measuring connecting tube on the syringe to the artery or vein of the experimental animal.

[0038] T2. Set the syringe extraction or infusion speed, syringe type and animal weight through the touch screen. The extraction or infusion speed is recommended to be below 20ml / min.

[0039] T3. Set the number of models through the touch screen, input the expected blood volume for each model, and set the holding time after reaching the expected blood volume through the touch screen. The expected blood volume value can be adjusted through automatic bloodletting or injection to achieve precise control of injection solution or blood extraction.

[0040] In T3, when the expected blood volume is extracted, the moving motor is started to drive the syringe to extract blood.

[0041] In T3, when the operation for the expected blood volume is perfusion, the mobile motor is started to drive the syringe to perfuse the experimental drug solution (such as saline). The mobile motor will continue to rotate in the perfusion direction until the set expected blood volume is perfused, and the mobile motor stops rotating until all models are completed.

[0042] During T3, the touch screen automatically displays the working status of the current experimental device (extraction, maintenance, irrigation), the current blood pressure value of the animal, the total perfusion volume (or blood loss), the blood pressure value status of the animal (or the degree of ischemia), and the adjustment capacity of each set model.

[0043] T4, the control system controls the mobile motor to drive the syringe to move according to the expected blood volume in T3, and receives the real-time blood pressure value recorded by the blood pressure transducer (response time <= 300 milliseconds / time). After reaching the expected blood volume, it enters the holding time, and after reaching the holding time, it enters the next model until all models are completed.

[0044] Therefore, the present invention adopts an experimental device of the above structure for replicating an ischemic animal model and a method of using the same, which improves the replication accuracy of the experimental model and the reliability of the experimental results, simplifies the operating process, reduces human errors, is suitable for various experimental needs, and improves experimental efficiency.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. An experimental device for replicating an ischemic animal model, characterized in that: It includes a base, a fixing mechanism and an adjusting mechanism. A touch display screen is arranged on the base. A fixing frame of the fixing mechanism is connected to the top of the base. The fixing frame is hinged to an adjusting screw of the adjusting mechanism. A movable seat threadedly connected to the adjusting screw is connected to a piston rod of a syringe. The syringe is fixedly connected to a fixing groove of the fixing frame. One end of the fixing frame away from the fixing groove is connected to a movable motor. The syringe is connected to a three-way valve through a pressure measuring connecting pipe. The three-way valve is connected to a blood pressure transducer.

2. The experimental device for replicating an ischemic animal model according to claim 1, characterized in that: The mobile motor is connected with the adjusting screw rod, and the limiting rods are symmetrically arranged on both sides of the adjusting screw rod. The limiting rods are slidably connected with the moving seat, and the two ends of the limiting rods are connected with the fixing frame. The two ends of the fixing frame are provided with limit switches outside the limiting rods.

3. The experimental device for replicating an ischemic animal model according to claim 1, characterized in that: The fixing groove on the fixing frame matches the shape of the bottom of the syringe, the locking frame at the rear end of the fixing groove is connected to the rear of the syringe, and the two sides of the locking frame are threadedly connected to the fixing frame through locking spring screws.

4. The experimental device for replicating an ischemic animal model according to claim 2, characterized in that: The touch display screen, limit switch and moving motor are all electrically connected to the control system, and the wireless Bluetooth component of the biological signal acquisition and processing system transmits signals to the control system.

5. A method for using the experimental device for replicating an ischemic animal model according to any one of claims 1 to 4, characterized in that: The automatic copying of the blood pressure model includes the following steps: S1. Use a syringe to draw 200 U / mL heparin saline, expel the air in the syringe and the pressure measuring tube connected to it, fix the syringe on the fixing frame through the locking frame, and connect the pressure measuring connecting tube on the syringe to the three-way valve; The other two interface channels of the three-way valve in S2 and S1 are connected to the blood pressure transducer and the animal artery respectively, and the syringe extraction or infusion speed, animal weight, and syringe specifications are set through the touch screen; S3. Setting the number of models for the ischemic animal experiment through the touch screen, and inputting the expected blood pressure value corresponding to each model, setting the blood pressure constant time through the touch screen, and the control system comparing the actual blood pressure value with the expected blood pressure value, and controlling the mobile motor to drive the syringe to work in real time according to the comparison result; S4. After each model reaches the expected blood pressure value and maintains the blood pressure constant for a period of time, it enters the next model and records the blood compensation volume of the previous model until all models are completed.

6. The method for using the experimental device for replicating an ischemic animal model according to claim 5, characterized in that: In S3, when the actual blood pressure value is greater than the expected blood pressure value, the mobile motor drives the syringe to draw blood until the expected blood pressure value is reached to keep the blood pressure constant.

7. The method for using the experimental device for replicating an ischemic animal model according to claim 5, characterized in that: In S3, when the actual blood pressure value is less than the expected blood pressure value, the mobile motor drives the syringe to infuse until the blood pressure reaches the expected blood pressure value and the blood pressure is kept constant.

8. A method for using the experimental device for replicating an ischemic animal model according to any one of claims 1 to 4, characterized in that: When performing automatic volume pumping and filling, the following steps are included: T1. Use a syringe to draw out the experimental drug solution and expel the air in the syringe and the pressure measuring connecting tube connected to it. Fix the syringe on the fixing frame through the locking frame and connect the pressure measuring connecting tube on the syringe to the artery or vein of the experimental animal. T2. Set the syringe extraction or infusion speed, syringe specifications and animal weight through the touch screen; T3. Set the number of models through the touch screen, input the expected blood volume for each model, and set the holding time after reaching the expected blood volume through the touch screen; T4, the control system controls the mobile motor to drive the syringe to move according to the expected blood volume in T3, and enters the holding time after reaching the expected blood volume. After reaching the holding time, it enters the next model until all models are completed.

9. The method for using the experimental device for replicating an ischemic animal model according to claim 8, characterized in that: In T3, the operation for the expected blood volume is to start the moving motor to drive the syringe to draw blood when drawing blood.

10. The method for using the experimental device for replicating an ischemic animal model according to claim 8, characterized in that: In T3, the operation for the expected blood volume is to start the moving motor to drive the syringe to perfuse the experimental drug solution during perfusion.

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