Method for increasing protein content of mulberry leaves

Abstract

The invention relates to the technical field of plant cultivation, in particular to a method for increasing the protein content of mulberry leaves, which comprises the following steps: after preparing a treating fluid, irrigating mulberry seeds with the treating fluid; the treating fluid is obtained by mixing 100 to 400mg of soybean lecithin and 1L of water; according to the method, the mulberry seeds are treated through the treating fluid, the mulberry seedlings with the high mulberry leaf protein content are obtained, after the obtained high-protein mulberry seedlings are transplanted for two years, the obtained mulberry leaves still maintain the high protein content, and the novel method which is easy to achieve and can effectively improve the protein content of the mulberry leaves is achieved.

Description

[Technical field]

[0001] The invention relates to the technical field of plant cultivation, and in particular to a method for increasing the protein content of mulberry leaves. [Background technology]

[0002] Mulberry is an important high-protein feed crop. The higher the protein content in mulberry leaves, the more beneficial it is to the growth and development of silkworms, the yield of cocoons and the quality of silk. Mulberry leaves have low crude fiber content and high crude protein content in dry matter, making them one of the low-cost protein resources. However, the protein content in most mulberry leaves cannot meet the growth needs of silkworms, so it is necessary to increase the protein content in mulberry leaves through certain technical means.

[0003] In the prior art, the method of increasing the protein content of mulberry trees is mainly achieved by breeding new high-protein mulberry varieties. However, breeding new varieties has high technical requirements, slow progress, long time period, and is difficult to promote.

[0004] Therefore, it is extremely urgent to develop a method for increasing the protein content of mulberry trees that is easily achievable, and the present invention arises at the historic moment. [Summary of the invention]

[0005] In view of the above, it is necessary to provide a method for increasing the protein content of mulberry leaves, and to realize a new method that is easy to implement and can effectively increase the protein content of mulberry leaves.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is:

[0007] A method for increasing the protein content of mulberry leaves, the method comprising preparing a treatment liquid and then watering mulberry seeds with the treatment liquid;

[0008] The treatment liquid is obtained by mixing soybean lecithin and water, and the solid-liquid ratio of the treatment liquid is 100-400 mg / L.

[0009] Furthermore, the preparation method of the treatment liquid is as follows: soybean lecithin is taken, water is added according to a certain proportion, and then ultrasonic treatment is performed for 20-30 minutes to obtain the treatment liquid. Soybean lecithin is commercially available under the brand name Meihe.

[0010] Furthermore, the method is specifically as follows: from the first day of germination of the mulberry seeds, the mulberry seeds are watered with the treatment liquid, once every 5 days, for a total of 3 times.

[0011] Furthermore, before watering, wood ash is added to the treatment liquid, and then the obtained mixture is used to water the mulberry seeds, and the amount of the added wood ash accounts for 3-6% of the total mass of the treatment liquid.

[0012] Furthermore, the mulberry seeds are first soaked in 2.8% H2O2 for 2-3 hours, then washed with clean water and sown in a seedling pot, and then transplanted to the field after cultivation for 31 days.

[0013] Each seedling pot was filled with 0.3 L of nutrient soil, which was purchased from Changchun Yiyang Sunny Gardening Co., Ltd. and contained 50% argillaceous carbon, 30% expanded perlite, 10% vermiculite, and 10% auxiliary materials.

[0014] Furthermore, the solid-liquid ratio of the treatment liquid is 200 mg / L.

[0015] Furthermore, the amount of the added wood ash accounts for 5% of the total mass of the treated liquid.

[0016] The present invention has at least the following beneficial effects:

[0017] 1. The present application treats mulberry seeds with a treatment liquid to obtain a mulberry seedling with a high mulberry leaf protein content. After the obtained high-protein mulberry seedling is transplanted for 2 years, the obtained mulberry leaves still maintain a high protein content. The present application realizes a new method that is easy to implement and can effectively increase the protein content of mulberry leaves.

[0018] 2. In order to solve the problem that the protein content of mulberry leaves cannot meet the needs of silkworm growth, the applicant has conducted many years of research and found that in the process of cultivating mulberry seeds into mulberry seedlings, watering the mulberry seeds with a treatment solution composed of soybean lecithin and water can promote the enrichment of protein in mulberry leaves and obtain mulberry seedlings with high protein content. After transplanting the mulberry seedlings, high-protein mulberry leaves can be harvested; during the experiment, the applicant also found that the protein content of mulberry seedlings was significantly different when treated with different concentrations of soybean lecithin, and the pattern presented was unpredictable. Therefore, the applicant conducted a large number of experiments to obtain the optimal soybean lecithin concentration value; in addition, the timing of watering also has an important influence on the protein enrichment of mulberry seedlings. After many years of relevant research experience combined with experiments, it was concluded that watering the treatment solution on the day when the mulberry seeds germinated can achieve the optimal protein enrichment.

[0019] 3. In the present application, although the protein content of mulberry seedlings can be increased after watering the seeds with a treatment solution composed of soybean lecithin, the growth of the roots of the mulberry seedlings will be inhibited, affecting the survival rate after transplantation and the growth quality of the mulberry trees. In this regard, the applicant has found that before watering, compounding an appropriate proportion of wood ash in the treatment solution can solve the above-mentioned problem and achieve mulberry seedlings with high protein content and good growth quality. [Specific implementation method]

[0020] In order to make the above-mentioned purpose, features and advantages of the present invention more obvious and easy to understand, the specific implementation of the present invention is described in detail below. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific implementation disclosed below.

[0021] Embodiment 1:

[0022] This embodiment provides a method for increasing the protein content of mulberry leaves;

[0023] The method comprises the following steps:

[0024] (1) preparing a treatment liquid: taking soybean lecithin, adding water thereto, and then ultrasonically treating for 20 minutes to obtain the treatment liquid; the solid-liquid ratio of the treatment liquid is 100 mg / L;

[0025] (2) Mixing plant ash: adding plant ash to the treatment liquid obtained in step (1) to obtain a watering liquid, wherein the amount of the added plant ash accounts for 3% of the total mass of the treatment liquid;

[0026] (3) Seed cultivation: soak the mulberry seeds in 2.8% H2O2 for 2 h, then wash them with clean water and sow them in a seedling pot. From the first day of germination, water the mulberry seeds with the watering solution obtained in step (2), once every 5 days, for a total of 3 times;

[0027] (4) Transplantation: Mulberry seeds are transplanted into the field after 31 days of cultivation.

[0028] Embodiment 2:

[0029] This embodiment provides a method for increasing the protein content of mulberry leaves;

[0030] The method comprises the following steps:

[0031] (1) preparing a treatment liquid: taking soybean lecithin, adding water thereto, and then ultrasonically treating for 25 minutes to obtain the treatment liquid; the solid-liquid ratio of the treatment liquid is 200 mg / L;

[0032] (2) Mixing plant ash: adding plant ash to the treatment liquid obtained in step (1) to obtain a watering liquid, wherein the amount of the added plant ash accounts for 5% of the total mass of the treatment liquid;

[0033] (3) Seed cultivation: soak the mulberry seeds in 2.8% H2O2 for 2.5 h, then wash them with clean water and sow them in a seedling pot. From the first day of germination, water the mulberry seeds with the watering solution obtained in step (2), once every 5 days, for a total of 3 times;

[0034] (4) Transplantation: Mulberry seeds are transplanted into the field after 31 days of cultivation.

[0035] Embodiment 3:

[0036] This embodiment provides a method for increasing the protein content of mulberry leaves;

[0037] The method comprises the following steps:

[0038] (1) preparing a treatment liquid: taking soybean lecithin, adding water thereto, and then ultrasonically treating for 30 minutes to obtain the treatment liquid; the solid-liquid ratio of the treatment liquid is 400 mg / L;

[0039] (2) Mixing plant ash: adding plant ash to the treated liquid obtained in step (1) to obtain a watering liquid, wherein the amount of the added plant ash accounts for 6% of the total mass of the treated liquid;

[0040] (3) Seed cultivation: soak the mulberry seeds in 2.8% H2O2 for 3 h, then wash them with clean water and sow them in a seedling pot. From the first day of germination, water the mulberry seeds with the watering solution obtained in step (2), once every 5 days, for a total of 3 times;

[0041] (4) Transplantation: Mulberry seeds are transplanted into the field after 31 days of cultivation.

[0042] Embodiment 4:

[0043] This embodiment provides a method for increasing the protein content of mulberry leaves;

[0044] The method comprises the following steps:

[0045] (1) preparing a treatment solution: taking 200 mg of soybean lecithin, adding 1 L of water thereto, and then ultrasonically treating for 25 min to obtain the treatment solution;

[0046] (2) Seed cultivation: soak the mulberry seeds in 2.8% H2O2 for 2.5 h, then wash them with clean water and sow them in a seedling pot. From the first day of germination, water the mulberry seeds with the treatment solution obtained in step (1), once every 5 days, for a total of 3 times;

[0047] (3) Transplantation: Mulberry seeds are transplanted into the field after 31 days of cultivation.

[0048] In order to illustrate the practical effect of the method for increasing the protein content of mulberry leaves described in the present application, the applicant conducted the following experiments:

[0049] Among them, the mulberry varieties selected for the following experiments are: Guisang No. 5, which is a mulberry variety bred by the Guangxi Zhuang Autonomous Region Sericulture Technology Promotion Station. It was approved by the Guangxi Zhuang Autonomous Region Crop Variety Approval Committee in 2015 (Approval No.: Guishensang 2015001) and has the basic common characteristics of mulberry trees.

[0050] Experiment 1: Detecting the growth of mulberry seedlings and the protein content of mulberry leaves in mulberry seedlings

[0051] Mulberry seeds were cultivated in the manner of Examples 1-4 and Comparative Examples 1-7, and mulberry seedlings were obtained after 31 days of cultivation. The growth conditions (plant height, root length, and fresh weight of a single plant) of each group of mulberry seedlings were recorded. Mulberry leaves of the mulberry seedlings obtained in each group were taken, and the total protein content in the mulberry leaves was determined by the Coomassie Brilliant Blue G-250 method.

[0052] Comparative Examples 1-7 are as follows:

[0053] Comparative Example 1: The solid-liquid ratio of the treatment liquid in this comparative example is 50 mg / L. Other methods are the same as those in Example 2.

[0054] Comparative Example 2: The solid-liquid ratio of the treatment liquid in this comparative example is 500 mg / L. Other methods are the same as those in Example 2.

[0055] Comparative Example 3: The treatment liquid was replaced with clean water. Other methods were the same as those in Example 2.

[0056] Comparative Example 4: The irrigation liquid was replaced with clean water (i.e., the treatment liquid and the wood ash were removed). Other methods were the same as those in Example 2.

[0057] Comparative Example 5: The timing of watering is from the first day of sowing the mulberry trees. Other than that, the other methods are the same as those in Example 2;

[0058] Comparative Example 6: The timing of watering is from the third day after the mulberry tree sprouts. Other methods are the same as those in Example 2.

[0059] Comparative Example 7: Tea seed cake was used to replace wood ash. Other than that, the other methods were the same as those in Example 2.

[0060] The growth of mulberry seedlings in the above groups and the detection of protein content in mulberry leaves are shown in Table 1:

[0061] Table 1 Mulberry seedling growth and mulberry leaf total protein content test results

[0062]

[0063]

[0064] According to the results in Table 1, it can be seen that the protein content of mulberry leaves in the obtained mulberry seedlings is high and the growth condition is good when the cultivation method of the present application is adopted. The present application proposes for the first time to apply soybean lecithin in the research on increasing the protein content of mulberry trees, which is of great significance. In the experimental project, the applicant found that no wood ash was added to the Example 4 group, and the protein content of mulberry leaves of the obtained mulberry seedlings could still be maintained at a relatively high level. However, the root length of the mulberry seedlings in Example 4 after removing the wood ash was restricted, and was only 3.56 cm. Combined with the comparative example 3 in which the treatment liquid was removed and the comparative example 4 in which the treatment liquid and the wood ash were removed, it was found that the addition of soybean lecithin would affect the development of the root length of the mulberry seedlings. In order to solve this problem, the applicant added wood ash and the treatment liquid to mix into an irrigation liquid and then irrigated the mulberry seeds, which ultimately ensured that the root length of the mulberry seedlings was significantly increased, thereby ensuring the survival rate of the mulberry trees after transplanting.

[0065] The above examples only express several embodiments of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the present invention. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the attached claims.

Claims

1. A method for increasing the protein content of mulberry leaves, characterized in that: The method comprises preparing a treatment liquid and then watering mulberry seeds with the treatment liquid; The treatment liquid is obtained by mixing soybean lecithin and water, and the solid-liquid ratio of the treatment liquid is 100-400 mg / L.

2. The method according to claim 1, characterized in that The preparation method of the treatment liquid is as follows: soybean lecithin is taken, water is added according to a certain proportion, and then ultrasonic treatment is performed for 20-30 minutes to obtain the treatment liquid.

3. The method according to claim 1, characterized in that The method specifically comprises the following steps: watering the mulberry seeds with the treatment solution from the first day after the mulberry seeds germinate, watering once every 5 days, and watering 3 times in total.

4. The method according to claim 3, characterized in that: Before watering, plant ash is added to the treatment liquid, and then the obtained mixture is used to water the mulberry seeds. The amount of the added plant ash accounts for 3-6% of the total mass of the treatment liquid.

5. The method according to claim 1, characterized in that The mulberry seeds are first soaked in 2.8% H2O2 for 2-3 hours, then washed with clean water and sown in a seedling pot, and then transplanted to the field after being cultivated for 31 days.

6. The method according to claim 1, characterized in that The solid-liquid ratio of the treatment liquid is 200 mg / L.

7. The method according to claim 3, characterized in that The amount of the added plant ash accounts for 5% of the total mass of the treated liquid.

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