Nattokinase embedding method

Abstract

The invention discloses a nattokinase embedding method, and belongs to the technical field of nattokinase embedding, and the nattokinase embedding method comprises the following steps: step 1, preparing an embedding solution containing an embedding material which is a polymer with biocompatibility; step 2, mixing nattokinase with the embedding solution to form a mixture; step 3, carrying out embedding treatment on the mixture, so that the embedding material coats the outer surface of the nattokinase to form a protective film, and obtaining an embedded product; by optimizing embedding conditions and processes, effective stability and long-term preservation of the nattokinase in an in-vitro environment are realized, and a wider prospect is developed for application of the nattokinase in the fields of foods, medicines and the like. Particularly, in the aspect of preventing and treating cardiovascular and cerebrovascular diseases, the demand of high-stability nattokinase products is increasingly increased, and powerful technical support is provided for the market.

Description

Technical Field

[0001] The invention relates to the technical field of nattokinase embedding, in particular to a nattokinase embedding method. Background Art

[0002] Nattokinase, as an alkaline serine protease, has attracted wide attention due to its powerful thrombolytic effect. Thrombolysis, i.e. dissolving blood clots, is an important means of preventing and treating cardiovascular and cerebrovascular diseases. Nattokinase shows strong fibrinolytic activity under appropriate conditions. Compared with commonly used thrombolytic drugs such as urokinase in clinical practice, its thrombolytic effect is more significant and has no toxic side effects.

[0003] The main mechanisms of nattokinase thrombolysis include directly dissolving fibrin into small molecule peptides, amino acids, etc., stimulating vascular endothelial cells to produce tissue plasminogen activator (t-PA), activating the conversion of prourokinase into urokinase in the body, and degrading and inactivating the inhibitor of plasminogen activator (PAI-1), thereby regulating fibrinolysis. These mechanisms work together to make nattokinase a highly potential thrombolytic drug. However, there are some limitations to nattokinase in practical applications. Its activity is easily affected in an in vitro environment. Factors such as temperature, pH value, and redox state may lead to reduced enzyme activity or even inactivation. This limits the application of nattokinase in food, medicine and other fields. In order to overcome these limitations, researchers began to explore the encapsulation technology of nattokinase. Encapsulation technology is a method of encapsulating active ingredients in a protective carrier to improve the stability and biological activity of the active ingredients. By encapsulation, nattokinase can be protected from adverse external factors, thereby maintaining its activity.

[0004] At present, a variety of encapsulation technologies have been applied to the stabilization research of nattokinase. These technologies include spray drying, microencapsulation, liposome encapsulation, etc. Each technology has its unique advantages and applicable scenarios, and the appropriate technology can be selected according to specific needs. Although the existing nattokinase encapsulation technology has improved the stability of the enzyme to a certain extent, there are still some problems. For example, some encapsulation materials may not have ideal biocompatibility, transparency or mechanical strength, which affects the encapsulation effect. In addition, some encapsulation methods may be complicated to operate and costly, which is not conducive to large-scale production and application. Summary of the invention

[0005] In order to solve the problems of the prior art, the present invention provides a method for embedding nattokinase, the method comprising the following steps:

[0006] Step 1: preparing an embedding solution containing an embedding material:

[0007] The embedding material is a biocompatible polymer;

[0008] Step 2, mixing nattokinase with the embedding solution to form a mixture;

[0009] Step 3: embedding the mixture so that the embedding material is coated on the outer surface of the nattokinase to form a protective film to obtain an embedded product.

[0010] Furthermore, in step 1, polyacrylic acid resin latex is selected as the embedding material.

[0011] Furthermore, the embedding solution also includes a solvent, and the solvent is selected from any one of talcum powder and triethyl citrate solution.

[0012] Furthermore, the amount of the solvent used is 10% to 30% of the embedding solution.

[0013] Furthermore, the embedding solution also includes a natto powder mixture;

[0014] The mass ratio of the natto powder mixture to the embedding material is 1:5;

[0015] The natto powder mixture comprises: natto and a traditional Chinese medicine component, wherein the traditional Chinese medicine component comprises astragalus, organic turmeric and ginseng, and the ratio of the astragalus, the astragalus, the organic turmeric and the ginseng is 40:30:30 in terms of mass ratio;

[0016] The ratio of the natto to the Chinese medicinal water is 70:30. The Chinese medicinal water is formed by mixing the Chinese medicinal components with water. When preparing the Chinese medicinal water, the amount of water added is 1 / 4 of the Chinese medicinal components.

[0017] The Chinese medicinal water is mixed with the natto and then formed into granules through low temperature drying.

[0018] Furthermore, in step 2, the amount of nattokinase added to the mixture is 5% to 20% of the embedding solution;

[0019] The mixing conditions are: stirring at 500-1000 rpm for 30-60 minutes at room temperature to evenly disperse the nattokinase in the embedding solution.

[0020] Further, in step 3, the embedding process is:

[0021] The mixture is sprayed to form droplets, and then the droplets are dried in an air flow at 25° C., so that the embedding material forms a dense protective film on the surface of nattokinase.

[0022] Furthermore, the method further comprises a post-processing process for the embedded product;

[0023] The post-treatment process includes heat treatment and / or drying treatment.

[0024] Further, the heat treatment is: heating the embedded product at 25-35° C. for 2 hours;

[0025] The drying process comprises: drying in a vacuum environment at a temperature of 25-35° C. for 22-26 hours.

[0026] Beneficial effects of the present invention:

[0027] This application achieves effective stabilization and long-term preservation of nattokinase in an in vitro environment by optimizing the embedding conditions and processes, opening up broader prospects for its application in the fields of food, medicine, etc. Especially in the prevention and treatment of cardiovascular and cerebrovascular diseases, the demand for high-stability nattokinase products is growing, providing strong technical support for the market.

[0028] By selecting high-performance encapsulation materials, we can effectively isolate adverse external factors, provide all-round protection for nattokinase, and significantly improve its environmental tolerance and long-term stability.

[0029] The whole embedding method is simple, easy and low-cost, suitable for large-scale industrial production, reducing production costs and improving economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The present invention provides a schematic flow diagram of the embedding method of nattokinase. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Example 1

[0033] See also Figure 1 The present invention provides a method for embedding nattokinase, comprising the following steps:

[0034] Step 1: Selection of embedding materials;

[0035] Polyacrylic acid resin latex (enteric soluble) was selected as the embedding material because of its good biocompatibility, transparency and mechanical strength.

[0036] The material exhibits excellent biocompatibility, ensuring harmonious coexistence with the biological environment; at the same time, its high transparency facilitates observation of the embedding effect, and its high mechanical strength ensures the structural stability of the embedded product. In addition, the material also has good heat resistance and chemical corrosion resistance, and can maintain stable performance under various environmental conditions, thereby effectively resisting the influence of adverse external factors on nattokinase.

[0037] Many preparations are irritating due to their drug properties, and some preparations become ineffective due to the effects of gastric juice or enzymes. Polyacrylic acid resin latex (enteric type) can be used for coating these preparations and can also control the release of the preparations in the gastrointestinal tract.

[0038] Polyacrylic acid resin latex (enteric type) contains carboxyl groups and is an anionic methacrylate polymer, so it has the properties of anionic compounds. When coated with this material, the drug is insoluble in acid and is released in an environment greater than pH 6.5, playing a role in localized release in the small intestine and colon. It has a low water vapor permeability, and drug release also depends on the coating thickness and the solubility of the drug in the physiological environment.

[0039] Polyacrylic acid resin latex (enteric type) is supplied in the form of aqueous dispersion and can be used as a coating material for common oral dosage forms, tablets, capsules and granules.

[0040] Step 2, preparing embedding solution;

[0041] The natto powder mixture and the embedding material are mixed at a ratio of 1:5 (mass ratio), and an appropriate amount of solvent is added to prepare a uniform and stable embedding solution;

[0042] The choice of solvent must ensure that the embedding material can be fully dissolved and maintain good compatibility with nattokinase. Water is selected as the solvent, which can effectively dissolve the polyacrylic acid resin latex.

[0043] The natto powder mixture comprises: natto and a traditional Chinese medicine component, wherein the traditional Chinese medicine component comprises astragalus, organic turmeric and ginseng, and the ratio of the astragalus, the astragalus, the organic turmeric and the ginseng is 40:30:30 in terms of mass ratio;

[0044] The ratio of the natto to the Chinese medicinal water is 70:30. The Chinese medicinal water is formed by mixing the Chinese medicinal components with water. When preparing the Chinese medicinal water, the amount of water added is 1 / 4 of the Chinese medicinal components.

[0045] The Chinese medicinal water is mixed with the natto and then formed into granules through low temperature drying.

[0046] Step 3, forming a mixture;

[0047] A purified and concentrated nattokinase solution is added to the embedding solution, and then stirred to ensure that the nattokinase is evenly dispersed in the embedding solution.

[0048] Step 4, drying the mixture;

[0049] The mixture of nattokinase and the embedding material is sprayed into tiny droplets by using spray drying technology, and then quickly dried in a high-temperature airflow of 30℃-40℃, preferably 35℃, so that the embedding material forms a dense protective film on the surface of nattokinase. During this process, the inlet temperature is 45℃ and the outlet temperature is 15℃ to avoid the adverse effects of high temperature on the activity of nattokinase and ensure that the embedding material is evenly covered and the film layer is intact.

[0050] Post-processing optimization:

[0051] The spray-dried product is subjected to fine post-processing, including a heat treatment process and a vacuum drying process.

[0052] The specific conditions are as follows:

[0053] Heat treatment process: heat treatment at 30℃-40℃ for 2 hours, preferably 35℃, to further enhance the stability of the embedded product. In this process, the internal structure of the embedded material is further optimized, and the combination with nattokinase is more closely.

[0054] Vacuum drying process: Dry for 24 hours at a vacuum degree of -0.1MPa and a temperature of 20℃-30℃, preferably 25℃, to effectively remove residual moisture and avoid potential damage to the embedded product due to high temperature.

[0055] The results showed that the encapsulated product of nattokinase was prepared by spray drying using polyacrylic acid resin latex as the encapsulation material. After stability testing, the results showed that the activity of the encapsulated nattokinase could still retain 85% of the original activity after being stored at 4°C for 3 months. This shows that PLA encapsulation material can effectively protect nattokinase from environmental factors, significantly improve its stability, and is suitable for occasions that require low-temperature storage.

[0056] Example 2

[0057] This embodiment uses chitosan as the embedding material because it has good biocompatibility and chemical corrosion resistance.

[0058] Solvent: Acetic acid solution is used as the solvent, which can effectively dissolve chitosan.

[0059] The rest of the steps are the same.

[0060] The results showed that chitosan was used as an embedding material and the embedded product of nattokinase was prepared by spray drying. The stability test showed that after the embedded nattokinase was stored at room temperature for 2 months, its activity could still retain 80% of the original activity. This verifies that chitosan embedding material can also effectively protect nattokinase under different storage conditions, improve its stability, and is suitable for a variety of storage environments.

[0061] Example 3

[0062] This embodiment uses polyethylene glycol (PEG) as the embedding material because it has good water solubility and stability.

[0063] Solvent: Water is used as the solvent. PEG can dissolve in water to form a uniform solution.

[0064] The rest of the steps are the same.

[0065] The results showed that the PEG-encapsulated product of nattokinase was prepared by spray drying using PEG as the encapsulation material. In the stability test, the activity of the encapsulated nattokinase was still 75% of the original activity after being stored at 37°C for 1 month. This shows that PEG-encapsulated materials are particularly suitable for the protection of nattokinase in high temperature environments, providing effective protection for the high temperature treatment process of nattokinase in the field of biomedicine and expanding its application range.

[0066] Through the above three embodiments, the feasibility and effectiveness of the nattokinase embedding technology of the present invention under different conditions are fully demonstrated. The selection and optimization process of different embedding materials ensure the high stability of nattokinase in various storage and application environments, laying a solid foundation for the wide application of nattokinase in the fields of food and medicine.

[0067] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for embedding nattokinase, characterized in that, The following steps are involved: Step 1: preparing an embedding solution containing an embedding material: The embedding material is a biocompatible polymer; Step 2, mixing nattokinase with the embedding solution to form a mixture; Step 3: embedding the mixture so that the embedding material is coated on the outer surface of the nattokinase to form a protective film to obtain an embedded product.

2. The embedding method of nattokinase according to claim 1, characterized in that In the step 1, polyacrylic acid resin latex is selected as the embedding material.

3. The embedding method of nattokinase according to claim 1, characterized in that The embedding solution also includes a solvent, and the solvent is selected from any one of water, talcum powder and triethyl citrate solution.

4. The embedding method of nattokinase according to claim 3, characterized in that The amount of the solvent used is 10% to 30% of the embedding solution.

5. The embedding method of nattokinase according to claim 1, characterized in that The embedding solution also includes a natto powder mixture; The mass ratio of the natto powder mixture to the embedding material is 1:5; The natto powder mixture comprises: natto and a traditional Chinese medicine component, wherein the traditional Chinese medicine component comprises astragalus, organic turmeric and ginseng, and the ratio of the astragalus, the astragalus, the organic turmeric and the ginseng is 40:30:30 in terms of mass ratio; The ratio of the natto to the Chinese medicinal water is 70:

30. The Chinese medicinal water is formed by mixing the Chinese medicinal components with water. When preparing the Chinese medicinal water, the amount of water added is 1 / 4 of the Chinese medicinal components. The Chinese medicinal water is mixed with the natto and then formed into granules through low temperature drying.

6. The embedding method of nattokinase according to claim 1, characterized in that In step 2, in the mixture, the amount of nattokinase added is 5% to 20% of the embedding solution; The mixing conditions are: stirring at 500-1000 rpm for 30-60 minutes at room temperature to evenly disperse the nattokinase in the embedding solution.

7. The embedding method of nattokinase according to claim 1, characterized in that In step 3, the embedding process is as follows: The mixture is sprayed to form droplets, and then the droplets are dried in an air flow at 30° C.-40° C., so that the embedding material forms a dense protective film on the surface of nattokinase.

8. The embedding method of nattokinase according to claim 1, characterized in that The method further comprises a post-processing process for the embedded product; The post-treatment process includes heat treatment and / or drying treatment.

9. The embedding method of nattokinase according to claim 8, characterized in that, The heat treatment is: heating the embedded product at 30-40° C. for 2 hours; The drying process comprises: drying in a vacuum environment at a temperature of 20-30° C. for 22-26 hours.

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