Long-acting slow-release agent capable of preventing and treating rumen acidosis of cattle and sheep

Abstract

The invention discloses a long-acting slow-release agent capable of preventing and treating rumen acidosis of cattle and sheep. The additive is prepared from the following components in parts by weight: 30-50 parts of sodium bicarbonate, 10-30 parts of magnesium oxide, 10-30 parts of activated carbon, 5-15 parts of bentonite, 2-5 parts of zinc amino acid chelate, 1-3 parts of selenium yeast, 1-3 parts of cobalt chloride, 2-3 parts of vitamin A, 2-4 parts of vitamin D, 1-3 parts of vitamin E and 10-20 parts of polylactic acid-glycolic acid copolymer (PLGA). The invention belongs to the technical field of livestock breeding, and particularly relates to a long-acting slow-release agent capable of preventing and treating rumen acidosis of cattle and sheep.

Description

Technical Field

[0001] The invention belongs to the technical field of animal husbandry, and specifically refers to a long-acting sustained-release agent capable of preventing and treating rumen acidosis of cattle and sheep. Background Art

[0002] Rumen acidosis is a common and serious problem in the breeding process of ruminants such as beef cattle and sheep. With the development of modern breeding technology, in order to improve the growth rate and meat production performance of cattle and sheep, farmers often increase the amount of concentrated feed. However, the concentrated feed ferments quickly in the rumen, which will lead to a large amount of acidic substances in the rumen, destroying the acid-base balance in the rumen, and then causing rumen acidosis.

[0003] Rumen acidosis can have a serious impact on the health and production performance of cattle and sheep. Affected cattle and sheep may experience symptoms such as loss of appetite, lethargy, weakened rumen motility, diarrhea, and even death in severe cases. This not only brings economic losses to farmers, but also affects the sustainable development of animal husbandry.

[0004] At present, although there are some products targeting rumen acidosis on the market, these products often have a short duration of action and require frequent additions, which increases breeding costs and labor intensity. In addition, the long-term and targeted effects of existing products in acid excretion and deacidification need to be improved, and they cannot fundamentally and permanently solve the problem of rumen acidosis. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep.

[0006] In order to achieve the above functions, the technical scheme adopted by the present invention is as follows: a long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep, the formula of which is composed of 30-50 parts of sodium bicarbonate, 10-30 parts of magnesium oxide, 10-30 parts of activated carbon, 5-15 parts of bentonite, 2-5 parts of amino acid chelated zinc, 1-3 parts of yeast selenium, 1-3 parts of cobalt chloride, 2-3 parts of vitamin A, 2-4 parts of vitamin D, 1-3 parts of vitamin E, and 10-20 parts of polylactic acid-glycolic acid copolymer (PLGA).

[0007] Preferably, the amino acid chelated zinc is fermented and cultured by Bacillus, and after the fermentation is completed, the fermentation broth containing the enzyme is collected, and zinc salt and amino acid are added to the reaction container, the pH value is adjusted to 6-7, the temperature is 30-40°C, and an appropriate amount of fermentation broth is added as an enzyme source, and the reaction is carried out for 4-6 hours. After the reaction is completed, the zinc is obtained by filtering, concentrating, drying, and the like.

[0008] Preferably, the preparation method of the yeast selenium is: a culture medium containing nutrients such as glucose, peptone, yeast extract, etc. is prepared by yeast strains, sodium selenite is added to the culture medium at a concentration of 10-50 mg / L, the yeast strains are inoculated, and fermentation is carried out at a temperature of 28-30°C and a pH value of 4.5-5.5. The fermentation time is 24-72 hours. As the fermentation proceeds, the yeast cells continuously absorb and convert selenium. After the fermentation is completed, the yeast cells are collected by centrifugation, washing, etc., and then dried to obtain yeast selenium.

[0009] The invention discloses a method for preparing a long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep, comprising the following steps:

[0010] (1) stirring sodium bicarbonate, magnesium oxide, activated carbon, bentonite, amino acid chelated zinc, yeast selenium, cobalt chloride, vitamin A, vitamin D and vitamin E in a mixer at room temperature at a speed of 30-60 r / min for 30-90 min to fully mix the ingredients and obtain a mixed powder;

[0011] (ii) dissolving PLGA in a suitable organic solvent to prepare a solution, and then performing step (i)

[0012] Slowly add the mixed powder into the solution, stir at 30-40°C and 50-80r / min for 60-120min to evenly disperse the powder in the solution to obtain a mixed solution;

[0013] (iii) spray drying the mixed solution in step (ii) by spray drying, wherein the inlet air temperature of the spray drying is set to 120-150°C, and the outlet air temperature is set to 60-80°C, to prepare a granular long-acting sustained-release agent with a particle size of 2-5 mm.

[0014] Preferably, the organic solvent in step (ii) is dichloromethane.

[0015] The present invention adopts the above structure to achieve the following beneficial effects: after cattle and sheep eat, the slow-release agent enters the rumen, sodium bicarbonate and magnesium oxide neutralize acidic substances, reduce acidic stimulation to the rumen mucosa, and relieve acidosis symptoms; activated carbon and bentonite adsorb acidic substances, toxins and harmful microorganisms, reduce damage to the rumen environment, facilitate the growth of beneficial microorganisms, and enhance buffering capacity; trace elements and vitamins are slowly released to provide nutrition for cattle, sheep and rumen microorganisms, maintain physiological functions, enhance immunity, promote balanced growth of microorganisms, and stabilize the internal environment; PLGA slowly degrades and continuously releases effective ingredients, maintains rumen acid-base balance and microecological stability for a long time, reduces the number of additions, reduces costs and labor intensity, and improves breeding efficiency. DETAILED DESCRIPTION

[0016] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Example 1

[0018] The invention discloses a method for preparing a long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep, comprising the following steps:

[0019] (i) Weigh 400 g of sodium bicarbonate, 200 g of magnesium oxide, 250 g of activated carbon, 100 g of bentonite, 30 g of amino acid chelated zinc, 20 g of yeast selenium, 20 g of cobalt chloride, 40 g of vitamin A, 30 g of vitamin D, and 30 g of vitamin E, and stir them in a mixer at a speed of 40 r / min for 45 min at room temperature to fully mix the ingredients to obtain a mixed powder;

[0020] (ii) weighing 180 g of PLGA and dissolving it in 750 g of dichloromethane to prepare a solution, then slowly adding the mixed powder in step (i) into the solution, stirring at 35° C. and 60 r / min for 75 min to uniformly disperse the powder in the solution to obtain a mixed solution;

[0021] (iii) spray drying the mixed solution in step (ii) by spray drying, wherein the inlet air temperature of the spray drying is set to 130° C. and the outlet air temperature is set to 70° C., to prepare a granular long-acting sustained-release agent with a particle size of 3 mm.

[0022] Effect verification

[0023] 20 healthy beef cattle were selected and randomly divided into two groups, each with 10 cattle. The long-acting sustained-release agent of the present invention was added to the feed of the test group beef cattle, and the addition amount was 50 g per cattle per day. The beef cattle in the control group were fed normally without adding the sustained-release agent, and were observed for 60 consecutive days, and the feeding conditions, rumen pH values, blood biochemical indicators, etc. of the beef cattle were recorded.

[0024] The results showed that the cattle in the experimental group had normal feeding, rumen pH values ​​were stable between 6.2-6.8, blood biochemical indicators were normal, and no symptoms of rumen acidosis appeared. Three cattle in the control group showed symptoms of acidosis such as loss of appetite and rumen pH values ​​below 5.5, and blood biochemical indicators were also abnormal.

[0025] Example 2

[0026] The invention discloses a method for preparing a long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep, comprising the following steps:

[0027] (i) Weigh 400 g of sodium bicarbonate, 200 g of magnesium oxide, 250 g of activated carbon, 100 g of bentonite, 30 g of amino acid chelated zinc, 20 g of yeast selenium, 20 g of cobalt chloride, 40 g of vitamin A, 30 g of vitamin D, and 30 g of vitamin E, and stir them in a mixer at a speed of 40 r / min for 45 min at room temperature to fully mix the ingredients to obtain a mixed powder;

[0028] (ii) weighing 180 g of PLGA and dissolving it in 750 g of dichloromethane to prepare a solution, then slowly adding the mixed powder in step (i) into the solution, stirring at 35° C. and 60 r / min for 75 min to uniformly disperse the powder in the solution to obtain a mixed solution;

[0029] (iii) spray drying the mixed solution in step (ii) by spray drying, wherein the inlet air temperature of the spray drying is set to 130° C. and the outlet air temperature is set to 70° C., to prepare a granular long-acting sustained-release agent with a particle size of 3 mm.

[0030] Effect verification

[0031] Thirty mutton sheep were selected and randomly divided into two groups, each with 15 sheep. The experimental group added a long-acting sustained-release agent to the feed, with the addition amount of 25g per sheep per day, and the control group was fed normally and observed for 45 consecutive days, and the rumen pH value, feces, weight changes, etc. of the mutton sheep were recorded.

[0032] The test results showed that the rumen pH value of the experimental group of mutton sheep was stable at 6.0-7.0, the feces were normal, and the weight gain was stable. Among the mutton sheep in the control group, 6 had abnormal feces, large fluctuations in rumen pH value (some were below 5.0), and the weight gain rate was lower than that of the experimental group.

[0033] The present invention and its implementation methods are described above, but such description is not restrictive, and what is shown is only one of the implementation methods of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.

Claims

1. A long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep, characterized in that: The formula composition by weight is: 30-50 parts of sodium bicarbonate, 10-30 parts of magnesium oxide, 10-30 parts of activated carbon, 5-15 parts of bentonite, 2-5 parts of amino acid chelated zinc, 1-3 parts of yeast selenium, 1-3 parts of cobalt chloride, 2-3 parts of vitamin A, 2-4 parts of vitamin D, 1-3 parts of vitamin E, and 10-20 parts of polylactic acid-glycolic acid copolymer (PLGA).

2. The long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep according to claim 1, characterized in that: The amino acid chelated zinc is fermented and cultured by Bacillus. After the fermentation is completed, the fermentation liquid containing the enzyme is collected, and zinc salt and amino acid are added to the reaction container. The pH value is adjusted to 6-7 and the temperature is 30-40° C., and an appropriate amount of fermentation liquid is added as an enzyme source. The reaction is carried out for 4-6 hours. After the reaction is completed, the zinc is obtained by filtering, concentrating, drying and the like.

3. The long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep according to claim 1, characterized in that: The preparation method of the yeast selenium comprises the following steps: preparing a culture medium containing nutrients such as glucose, peptone, yeast extract, etc. by using yeast strains, adding sodium selenite to the culture medium at a concentration of 10-50 mg / L, inoculating the yeast strains, and fermenting and culturing the culture medium at a temperature of 28-30° C. and a pH value of 4.5-5.5 for 24-72 hours. As the fermentation proceeds, the yeast cells continuously absorb and convert the selenium element. After the fermentation is completed, the yeast cells are collected by centrifugation, washing, etc., and then dried to obtain the yeast selenium.

4. A method for preparing a long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep according to any one of claims 1 to 3, characterized in that: The steps include: (1) stirring sodium bicarbonate, magnesium oxide, activated carbon, bentonite, amino acid chelated zinc, yeast selenium, cobalt chloride, vitamin A, vitamin D and vitamin E in a mixer at room temperature at a speed of 30-60 r / min for 30-90 min to fully mix the ingredients and obtain a mixed powder; (ii) dissolving PLGA in a suitable organic solvent to prepare a solution, then slowly adding the mixed powder in step (i) into the solution, stirring at 30-40° C. and 50-80 r / min for 60-120 min to uniformly disperse the powder in the solution to obtain a mixed solution; (iii) spray drying the mixed solution in step (ii) by spray drying, wherein the inlet air temperature of the spray drying is set to 120-150°C, and the outlet air temperature is set to 60-80°C, to prepare a granular long-acting sustained-release agent with a particle size of 2-5 mm.

5. The long-acting sustained-release agent capable of preventing and treating rumen acidosis in cattle and sheep according to claim 4, characterized in that: The organic solvent in the step (ii) is dichloromethane.