Composition, preparation method thereof and application of composition in preparation of medicine with sleep aiding effect

Abstract

The invention relates to the technical field of biological medicines, and particularly discloses a composition, a preparation method thereof and application of the composition in preparation of a medicine with a sleep aiding effect. The composition comprises lavender essential oil, bergamot essential oil and mint essential oil. Researches show that the composition containing the lavender essential oil, the bergamot essential oil and the mint essential oil has a sleep aiding effect; therefore, the compound can be further used as an effective component for preparing a medicine with a sleep aiding effect. In addition, the composition disclosed by the invention contains essential oil with a volatile effect; therefore, in the application process, the sleep aiding effect can be achieved through aromatherapy.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a composition and a preparation method thereof, and application of the composition in preparing a medicine having a sleep-aiding effect. Background Art

[0002] Insomnia is usually caused by excessive mental burden, mental work, improper long-term balance between work and rest, and physical weakness after illness. Long-term insomnia can lead to decreased memory and attention, which in turn affects work and study. Intervention measures for insomnia mainly include drug treatment and non-drug treatment.

[0003] Chinese utility model CN201520397493.7 discloses a tranquilizing insomnia patch; it includes: non-woven fabric, Chinese medicine patch, small holes, medical tape; the medicine core is made of specific Chinese medicine, which has significant effects on insomnia, neurasthenia, frequent dreams and easy awakening, malaise, memory loss, etc. It can not only be attached to the navel (Shenque point), but also to the soles of both feet (Yongquan point), Anmian point, and Dazhui point; the area of ​​the medical tape is reduced in structure, and it is replaced by a non-woven fabric with small holes. It is more breathable when used and fits the skin, especially suitable for use in summer. The medicine core is made of Coptis chinensis, cinnamon, Schisandra chinensis, cinnabar, Chinese yam, Curcuma aromatica, Salvia miltiorrhiza, Ziziphus jujuba seeds, Polygala tenuifolia, pearl powder, bee powder, and donkey-hide gelatin. However, the medicine core made of the above Chinese medicine is not suitable for all patients. Therefore, it is of great application value to develop more Chinese medicine compositions with the effect of treating insomnia. Summary of the invention

[0004] In order to overcome the technical problems existing in the prior art, the present invention first provides a composition; The above technical problem to be solved by the present invention is achieved by adopting the following technical solution: The present invention first provides a composition comprising lavender essential oil, bergamot essential oil and Peppermint essential oil.

[0005] The invention provides a new composition. Studies have shown that the composition comprising lavender essential oil, bergamot essential oil and peppermint essential oil has a sleep-aiding effect.

[0006] Preferably, the composition comprises the following components in parts by weight: 5-10 parts of lavender essential oil; 5-10 parts of bergamot essential oil; 3-5 parts of peppermint essential oil.

[0007] Most preferably, the composition comprises the following components in parts by weight: 8 parts of lavender essential oil; 8 parts of bergamot essential oil; 4 parts of peppermint essential oil.

[0008] Preferably, the composition further comprises red peony root extract.

[0009] The inventors found in further research that in the composition containing lavender essential oil, bergamot essential oil and The peppermint essential oil composition is further added with red peony root extract, and its sleep-inducing effect is further significantly improved.

[0010] Preferably, the composition comprises the following components in parts by weight: 5-10 parts of lavender essential oil; 5-10 parts of bergamot essential oil; 3-5 parts of peppermint essential oil; 4-6 parts of red peony root extract.

[0011] Most preferably, the composition is characterized in that it comprises the following components in parts by weight: 8 parts of lavender essential oil; 8 parts of bergamot essential oil; 4 parts of peppermint essential oil; 5 parts of red peony root extract.

[0012] Preferably, the red peony root extract is prepared by the following method: The Chinese medicinal material red peony root is extracted with a solvent, and after the extraction is completed, the extract is filtered and separated, and the extract is concentrated and dried to obtain a solvent extract, and the solvent extract is taken to obtain the red peony root extract.

[0013] Preferably, the solvent is diethyl ether.

[0014] The inventors found in further research that during the preparation of the red peony root extract of the present invention, The choice of solvent is critical; studies have shown that in a mixture containing lavender essential oil, bergamot essential oil and In the composition of peppermint essential oil, only when the red peony root extract extracted by ether is added, the sleep-inducing effect can be further significantly improved; however, when the red peony root extract extracted by other solvents is added, the sleep-inducing effect cannot be further significantly improved.

[0015] Preferably, the extraction is heating reflux extraction; the extraction time is 1 to 3 hours.

[0016] Preferably, the dosage ratio of the Chinese medicine red peony root to the solvent is 1kg:8~20L.

[0017] Preferably, the preparation method of the red peony root extract further comprises the following steps: The solvent extract is loaded onto a silica gel column, and eluted with a mixed solvent consisting of chloroform and methanol in a volume ratio of 100:5-7 for 3-5 times the column volume to obtain an eluent, and the eluent is concentrated and dried to obtain the red peony root extract.

[0018] Most preferably, the method for preparing the red peony root extract further comprises the following steps: The solvent extract is loaded onto a silica gel column, and eluted with a mixed solvent consisting of chloroform and methanol in a volume ratio of 100:6 with a volume ratio of 4 times the column volume to obtain an eluate, and the eluate is concentrated and dried to obtain the red peony root extract.

[0019] In further research, the inventors found that in a composition comprising lavender essential oil, bergamot essential oil and peppermint essential oil, adding a red peony root extract prepared by the above-mentioned silica gel column elution conditions, compared with adding an extract obtained only by ether extraction; its sleep-inducing effect is further significantly improved.

[0020] The inventors found in the study that the elution conditions of the silica gel column of the present invention are very critical; in a composition containing lavender essential oil, bergamot essential oil and peppermint essential oil, only by adding the red peony root extract prepared by the above-mentioned silica gel column elution conditions of the present invention, compared with adding the extract obtained only by ether extraction; its sleep-inducing effect can be further significantly improved; however, by adding the red peony root extract prepared by other silica gel column elution conditions, its sleep-inducing effect cannot be further significantly improved.

[0021] The present invention also provides an application of the traditional Chinese medicine composition in preparing a medicine having a sleep-aiding effect.

[0022] Beneficial effects: The present invention provides a new composition. Studies have shown that the composition comprising lavender essential oil, bergamot essential oil and peppermint essential oil has a sleep-inducing effect; therefore, it can be further used as an effective ingredient to prepare a drug with a sleep-inducing effect. In addition, since the composition of the present invention comprises essential oils with volatile effects, the sleep-inducing effect can be achieved through aromatherapy during the application process. DETAILED DESCRIPTION

[0023] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] The lavender essential oil, bergamot essential oil and peppermint essential oil described in the following examples are all lavender essential oil, bergamot essential oil and peppermint essential oil prepared by conventional steam distillation.

[0025] Example 1 Preparation of composition 8 parts of lavender essential oil, 8 parts of bergamot essential oil and 4 parts of peppermint essential oil are mixed evenly to obtain the composition.

[0026] Example 2 Preparation of composition 8 parts of lavender essential oil, 8 parts of bergamot essential oil, 4 parts of peppermint essential oil and 5 parts of red peony root extract are mixed evenly to obtain the composition; The red peony root extract is prepared by the following method: Take the Chinese herbal medicine red peony root, heat it to boiling with a solvent, and then perform reflux extraction for 1 hour. After the extraction is completed, filter and separate the extract, concentrate and dry the extract to obtain a solvent extract, and take the solvent extract to obtain the red peony root extract; Wherein, the solvent is ether; the dosage ratio of red peony root to the solvent is 1kg:15L.

[0027] Example 3 Preparation of composition 8 parts of lavender essential oil, 8 parts of bergamot essential oil, 4 parts of peppermint essential oil and 5 parts of red peony root extract are mixed evenly to obtain the composition; The red peony root extract is prepared by the following method: (1) taking a Chinese herbal medicine red peony root, heating it to boiling with a solvent, and then performing reflux extraction for 1 hour. After the extraction is completed, filtering and separating the extract, concentrating and drying the extract to obtain a solvent extract, and taking the solvent extract to obtain the red peony root extract; wherein the solvent is ether; and the ratio of red peony root to solvent is 1kg:15L; (2) The solvent extract is loaded onto a silica gel column, and eluted with a mixed solvent consisting of chloroform and methanol in a volume ratio of 100:6 with a volume ratio of 4 times the column volume to obtain an eluate, and the eluate is concentrated and dried to obtain the red peony root extract.

[0028] Comparative Example 1 Preparation of the composition 8 parts of lavender essential oil, 8 parts of bergamot essential oil, 4 parts of peppermint essential oil and 5 parts of red peony root extract are mixed evenly to obtain the composition; The red peony root extract is prepared by the following method: Take the Chinese herbal medicine red peony root, heat it to boiling with a solvent, and then perform reflux extraction for 1 hour. After the extraction is completed, filter and separate the extract, concentrate and dry the extract to obtain a solvent extract, and take the solvent extract to obtain the red peony root extract; Wherein, the solvent is water; the dosage ratio of red peony root to the solvent is 1kg:15L.

[0029] Comparative Example 2 Preparation of the composition 8 parts of lavender essential oil, 8 parts of bergamot essential oil, 4 parts of peppermint essential oil and 5 parts of red peony root extract are mixed evenly to obtain the composition; The red peony root extract is prepared by the following method: Take the Chinese herbal medicine red peony root, heat it to boiling with a solvent, and then perform reflux extraction for 1 hour. After the extraction is completed, filter and separate the extract, concentrate and dry the extract to obtain a solvent extract, and take the solvent extract to obtain the red peony root extract; Wherein, the solvent is methanol; the dosage ratio of red peony root to the solvent is 1kg:15L.

[0030] Comparative Example 3 Preparation of Composition 8 parts of lavender essential oil, 8 parts of bergamot essential oil, 4 parts of peppermint essential oil and 5 parts of red peony root extract are mixed evenly to obtain the composition; The red peony root extract is prepared by the following method: Take the Chinese herbal medicine red peony root, heat it to boiling with a solvent, and then perform reflux extraction for 1 hour. After the extraction is completed, filter and separate the extract, concentrate and dry the extract to obtain a solvent extract, and take the solvent extract to obtain the red peony root extract; Wherein, the solvent is ethyl acetate; the dosage ratio of red peony root to the solvent is 1kg:15L.

[0031] Comparative Example 4 Preparation of Composition 8 parts of lavender essential oil, 8 parts of bergamot essential oil, 4 parts of peppermint essential oil and 5 parts of red peony root extract are mixed evenly to obtain the composition; The red peony root extract is prepared by the following method: (1) taking a Chinese herbal medicine red peony root, heating it to boiling with a solvent, and then performing reflux extraction for 1 hour. After the extraction is completed, filtering and separating the extract, concentrating and drying the extract to obtain a solvent extract, and taking the solvent extract to obtain the red peony root extract; wherein the solvent is ether; and the ratio of red peony root to solvent is 1kg:15L; (2) The solvent extract is loaded onto a silica gel column, and eluted with chloroform 4 times the column volume to obtain an eluate, and the eluate is concentrated and dried to obtain the red peony root extract.

[0032] Comparative Example 5 Preparation of Composition 8 parts of lavender essential oil, 8 parts of bergamot essential oil, 4 parts of peppermint essential oil and 5 parts of red peony root extract are mixed evenly to obtain the composition; The red peony root extract is prepared by the following method: (1) taking a Chinese herbal medicine red peony root, heating it to boiling with a solvent, and then performing reflux extraction for 1 hour. After the extraction is completed, filtering and separating the extract, concentrating and drying the extract to obtain a solvent extract, and taking the solvent extract to obtain the red peony root extract; wherein the solvent is ether; and the ratio of red peony root to solvent is 1kg:15L; (2) The solvent extract is loaded onto a silica gel column, and eluted with a mixed solvent consisting of chloroform and methanol in a volume ratio of 100:15 with a volume ratio of 4 times the column volume to obtain an eluate, and the eluate is concentrated and dried to obtain the red peony root extract.

[0033] Experimental Example 1 70 Kunming male mice weighing 25±2g were selected and randomly divided into 7 groups, with 10 mice in each group; including a blank control group and experimental groups 1-6; the experimental groups 1-6 were smeared with the compositions prepared in Examples 1-4 and Comparative Examples 1-2 (at a dose of 15 mg / kg) on ​​the abdomen once a day; the blank control group was not smeared with any drug for 30 consecutive days; 40 minutes after the last application of the drug, each group of mice was intraperitoneally injected with sodium barbital at a dose of 50 mg / kg; the sleep latency (time from the injection of sodium barbital to falling asleep) and the sleep duration (time from falling asleep to waking up) of each group of mice were recorded; the experimental results are shown in Table 1.

[0034] Table 1.

[0035] The experimental results in Table 1 show that the sleep latency of mice in experimental group 1 is significantly shorter than that in the blank control group, and the sleep duration is significantly longer than that in the blank control group; this indicates that the composition of the present invention comprising lavender essential oil, bergamot essential oil and peppermint essential oil has a sleep-inducing effect.

[0036] The experimental results in Table 1 show that the sleep latency of mice in experimental group 2 was significantly higher than that in experimental group 1. The results showed that the sleep duration of mice in experimental group 2 was significantly shortened compared with that in experimental group 1, which indicated that the sleep-inducing effect of red peony root extract was further significantly improved by adding it to the composition containing lavender essential oil, bergamot essential oil and peppermint essential oil.

[0037] The experimental results in Table 1 show that the sleep latency of mice in experimental groups 4 to 6 was significantly lower than that in experimental group 1. Although it was shortened, the shortening was not significant at that time; and although the sleep duration of mice in experimental groups 4 to 6 was prolonged compared with that in experimental group 1, the extension was also not significant; this shows that: in the composition containing lavender essential oil, bergamot essential oil and peppermint essential oil, only by adding red peony root extract obtained by ether extraction, its sleep-aiding effect can be further significantly improved; however, by adding red peony root extract extracted by other solvents, its sleep-aiding effect cannot be further significantly improved.

[0038] The experimental results in Table 1 show that the sleep latency of mice in experimental group 3 is further significantly shorter than that in experimental group 2, and the sleep duration is further longer than that in experimental group 2; this indicates that: in the composition comprising lavender essential oil, bergamot essential oil and peppermint essential oil, the addition of red peony root extract prepared by the above-mentioned silica gel column elution conditions, compared with the addition of the extract obtained only by ether extraction; its sleep-inducing effect is further significantly improved.

[0039] The experimental results in Table 1 show that the sleep latency of mice in experimental groups 7 and 8 is not shortened much compared with that in experimental group 2, and the shortening is much smaller than that in experimental group 3; and the sleep duration of mice in experimental groups 7 and 8 is not extended much compared with that in experimental group 2, and the extension is much smaller than that in experimental group 3; this indicates that the elution conditions of the silica gel column of the present invention are very critical; in the composition comprising lavender essential oil, bergamot essential oil and peppermint essential oil, only by adding the red peony root extract prepared by the above-mentioned silica gel column elution conditions of the present invention, compared with adding the extract obtained by ether extraction only; its sleep-aiding effect can be further significantly improved; however, by adding the red peony root extract prepared by other silica gel column elution conditions, its sleep-aiding effect cannot be further significantly improved.

[0040] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0041] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A composition, characterized in that Contains lavender, bergamot and peppermint essential oils.

2. The composition according to claim 1, characterized in that Contains the following components in parts by weight: 5-10 parts of lavender essential oil; 5-10 parts of bergamot essential oil; 3-5 parts of peppermint essential oil.

3. The composition according to claim 1, characterized in that Contains the following components in parts by weight: 8 parts of lavender essential oil; 8 parts of bergamot essential oil; 4 parts of peppermint essential oil.

4. The composition according to claim 1, characterized in that The composition also contains red peony root extract.

5. The composition according to claim 4, characterized in that Contains the following components in parts by weight: 5-10 parts of lavender essential oil; 5-10 parts of bergamot essential oil; 3-5 parts of peppermint essential oil; 4-6 parts of red peony root extract.

6. The composition according to claim 4, characterized in that Contains the following components in parts by weight: 8 parts of lavender essential oil; 8 parts of bergamot essential oil; 4 parts of peppermint essential oil; 5 parts of red peony root extract.

7. The composition according to claim 4, characterized in that The red peony root extract Prepared by the following method: The Chinese medicinal material red peony root is extracted with a solvent, and after the extraction is completed, the extract is filtered and separated, and the extract is concentrated and dried to obtain a solvent extract, and the solvent extract is taken to obtain the red peony root extract.

8. The composition according to claim 7, characterized in that The solvent is diethyl ether.

9. The composition according to claim 7, characterized in that The extraction is heated back Flow extraction; extraction time is 1~3h.

10. Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a drug having a sleep-inducing effect.

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