A method of nucleic acid processing

Abstract

The embodiment of the present application provides a nucleic acid processing method, and relates to the technical field of biomolecule detection. The nucleic acid processing method comprises the following steps: in the extraction process of the nucleic acid, the magnetic beads can be stirred, the magnetic beads can be scattered in liquid vortex, the magnetic beads can be suspended under the action of a magnetic field, and the magnetic beads cannot fall into other channels after moving away from the magnetic field, so that the loss of the magnetic beads is reduced, the nucleic acid extraction can be completed, the accuracy of inspection is improved, high-precision nucleic acid extraction is realized, and high-precision nucleic acid amplification can also be realized.

Description

Technical Field

[0001] The present invention relates to the technical field of biomolecule detection, and in particular to a method for processing nucleic acid. Background Art

[0002] Microfluidics, also known as lab-on-a-chip, is a technology that manipulates fluids at the micrometer scale. This technology has miniaturized the basic functions of chemical and biological laboratories onto a chip just a few square centimeters in size.

[0003] Through the intersection of analytical chemistry, micro-electromechanical processing, computer science, electronics, materials science, biology, medicine and other disciplines, miniaturization, automation, integration and portability from sample processing to detection are achieved.

[0004] In the existing technology, it is difficult to achieve high-precision nucleic acid extraction through microfluidic chips. Summary of the Invention

[0005] The present invention provides a nucleic acid processing method, which can complete nucleic acid extraction, improve the accuracy of inspection, and realize high-precision nucleic acid extraction.

[0006] The embodiments of the present invention can be implemented as follows:

[0007] The present invention provides a method for processing nucleic acid, comprising:

[0008] S1: placing a microfluidic chip in an analyzer, wherein the microfluidic chip has an information extraction chamber and a first liquid storage chamber, a second liquid storage chamber, a third liquid storage chamber, and a fourth liquid storage chamber connected to the information extraction chamber, the information extraction chamber contains a plurality of magnetic beads, and the first liquid storage chamber, the second liquid storage chamber, the third liquid storage chamber, and the fourth liquid storage chamber respectively contain a lysate, a first washing solution, a second washing solution, and an eluent;

[0009] S2: adding a sample to the information extraction chamber;

[0010] S3: transporting the lysate in the first liquid storage chamber to the information extraction chamber;

[0011] S4: controlling the microfluidic chip to rotate at a first preset speed for a first preset time;

[0012] S5: controlling the magnetic steel magnetic member in the analyzer to be close to the microfluidic chip so that the magnetic beads are suspended in the information extraction chamber; wherein the method of step S5 further includes:

[0013] S501: Controlling the microfluidic chip to rotate in a forward direction by accelerating to a fourth preset speed and then decelerating for a third preset time;

[0014] S502: controlling the microfluidic chip to move at a third preset time in a manner of reverse acceleration to the fourth preset speed and then deceleration;

[0015] S503: repeating steps S501-S502 to generate vortex in the liquid in the information extraction chamber;

[0016] S6: controlling the rotation speed of the microfluidic chip to run at a fifth preset speed at a constant speed, so that the magnetic beads gather towards the side wall in the information extraction chamber;

[0017] S7: controlling the magnetic steel magnetic piece to rise away from the microfluidic chip, so that the magnetic beads stop suspending;

[0018] S8: heating the temperature control air cavity to make the air in the temperature control air cavity expand and enter the information extraction chamber, and the gas in the temperature control air cavity is discharged, wherein the microfluidic chip further has a temperature control air cavity, and the temperature control air cavity and the information extraction chamber are communicated;

[0019] S9: cooling the temperature control air cavity to make the temperature control air cavity in a negative pressure state to suck the liquid in the information extraction chamber into the temperature control air cavity, so that the magnetic beads and the nucleic acid adsorbed by the magnetic beads remain in the information extraction chamber;

[0020] S10: adding the first washing agent in the second liquid storage chamber into the information extraction chamber, and repeating steps S4-S9;

[0021] S11: adding the second washing agent in the third liquid storage chamber into the information extraction chamber, and repeating steps S4-S7;

[0022] S12: adding the eluent in the fourth liquid storage chamber into the information extraction chamber, and repeating steps S4-S7 to make the nucleic acid on the magnetic beads separate.

[0023] In an optional embodiment, after step S12, the nucleic acid processing method further comprises:

[0024] S13: heating the temperature control chamber to make the air in the temperature control chamber expand and enter the information extraction chamber to make the gas in the temperature control chamber discharge, wherein the microfluidic chip further has a detection chamber and a temperature control chamber, and the information extraction chamber, the detection chamber and the temperature control chamber are communicated in sequence;

[0025] S14: cooling the temperature control chamber to make the temperature control chamber in a negative pressure state to send the eluent with nucleic acid in the information extraction chamber to the detection chamber;

[0026] S15: heating the detection chamber under a preset temperature condition to realize nucleic acid amplification.

[0027] In an optional embodiment, the step S14 comprises:

[0028] S141: cooling the temperature-controlled room so that the temperature-controlled room is in a negative pressure state;

[0029] S142: controlling the microfluidic chip to rotate at a second preset speed, so that the liquid in the detection chamber enters the detection pool of the detection chamber under the action of centrifugal force;

[0030] Alternatively, after controlling the microfluidic chip to rotate at a third preset speed for a second preset time, controlling the microfluidic chip to rotate at the second preset speed for the second preset time, and then controlling the chip to rotate at the third preset speed for the second preset time, and so on, so that the liquid in the detection chamber enters the detection pool of the detection chamber under the action of centrifugal force, the third preset speed is less than the second preset speed.

[0031] In an optional embodiment, the second preset time is 0-10s, the second preset speed is 600-6000rpm, and the third preset speed is 0-1000rpm.

[0032] In an optional embodiment, the preset temperature condition is 60-95℃.

[0033] In an optional embodiment, the first preset speed is 100-6000rpm, and the first preset time is 1-700s.

[0034] In an optional embodiment, the diameter of the magnetic beads is 200-5000nm, and the fourth preset speed is 200-3000rpm.

[0035] In an optional embodiment, the method of step S5 further comprises:

[0036] Controlling the information extraction chamber to be warmed up to above 37℃.

[0037] In an optional embodiment, the diameter of the magnetic beads is 100-5000nm.

[0038] In an optional embodiment, the fifth preset speed is 100rpm-900rpm.

[0039] The nucleic acid processing method of the embodiments has the following beneficial effects, for example:

[0040] The nucleic acid processing method provided in the embodiment of the present application comprises: S1, placing a microfluidic chip in an analyzer; S2, adding a sample into an information extraction chamber; S3, transporting a lysis solution in a first liquid storage chamber to the information extraction chamber; S4, controlling the microfluidic chip to rotate at a first preset speed for a first preset time; S5, controlling a magnetic steel magnetic piece in the analyzer to approach the microfluidic chip, so that the magnetic beads are suspended in the information extraction chamber; S6, controlling the rotation speed of the microfluidic chip to run at a fifth preset speed at a constant speed, so that the magnetic beads gather towards the side wall in the information extraction chamber; S7, controlling the magnetic steel magnetic piece to rise away from the microfluidic chip, so that the magnetic beads stop being suspended; S8, heating a temperature control air cavity, so that the air in the temperature control air cavity expands and enters the information extraction chamber, and the gas in the temperature control air cavity is discharged, wherein the microfluidic chip further has a temperature control air cavity, and the temperature control air cavity and the information extraction chamber are communicated; S9, refrigerating the temperature control air cavity, so that the temperature control air cavity is in a negative pressure state, to suck the liquid in the information extraction chamber into the temperature control air cavity, so that the magnetic beads remain in the information extraction chamber; S10, adding a first washing agent in a second liquid storage chamber into the information extraction chamber, and repeating steps S4-S9; S11, adding a second washing agent in a third liquid storage chamber into the information extraction chamber, and repeating steps S4-S9; S12, adding an elution solution in a fourth liquid storage chamber into the information extraction chamber, and repeating steps S4-S7, so that the nucleic acid on the magnetic beads is separated, wherein the method of step S5 further comprises: S501, controlling the microfluidic chip to move at a positive acceleration rotation to a fourth preset speed and then decelerate for a third preset time; S502, controlling the microfluidic chip to move at a reverse acceleration rotation to the fourth preset speed and then decelerate for the third preset time; S503, repeating steps S501-S502, so that the liquid in the information extraction chamber generates a vortex, the nucleic acid processing method can stir the magnetic beads in the extraction process of the nucleic acid, the magnetic beads can be scattered in the liquid vortex, the magnetic beads can be suspended under the action of the magnetic field, and can not fall into other channels after moving away from the magnetic field, so as to reduce the loss of the magnetic beads, the nucleic acid extraction can be completed, the accuracy of the inspection is improved, and high-precision nucleic acid extraction is realized. BRIEF DESCRIPTION OF DRAWINGS

[0041] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0042] Figure 1 The schematic diagram of the microfluidic chip provided in the embodiment of the present application.

[0043] Icon: 10 microfluidic chip; 11 chip body; 12 information extraction chamber; 121 bottom wall; 122 side wall; 13 temperature control air cavity; 14 siphon pipe; 15 rotation center; 16 detection chamber; 161-quantitative pool; 162-detection pool; 17 temperature control chamber; 18-first liquid storage chamber; 19-second liquid storage chamber; 20-third liquid storage chamber; 21-fourth liquid storage chamber. DETAILED DESCRIPTION

[0044] To make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some but not all of the embodiments of the present application. The components of the embodiments of the present application described and shown in the drawings can be arranged and designed in various different configurations.

[0045] Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present application without creative labor are within the scope of protection of the present application.

[0046] It should be noted that: similar reference numerals and letters represent similar items in the following drawings, therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0047] In the description of the present application, it should be noted that if the terms "upper", "lower", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship when the product of the present application is usually placed, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, therefore, it cannot be understood as a limitation on the present application.

[0048] In addition, if the terms "first", "second" and the like appear, they are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0049] It should be noted that the features in the embodiments of the present application can be combined with each other without conflict.

[0050] Microfluidic chip has the characteristics of flexible combination of various unit technologies and large-scale integration, so that a large amount of information can be obtained with a small amount of sample, and it is more likely to exceed a single analysis function and become a whole micro multi-element operation platform. Because the microfluidic chip also has the advantages of small size, less reagent consumption, fast analysis speed, easy integration, etc., it provides a broad prospect for its application in many fields such as biomedical research, drug synthesis screening, environmental monitoring, health quarantine, etc.

[0051] As mentioned in the background art, microfluidic chip, also known as chip laboratory, is a technology for manipulating fluid at the micron scale. This technology miniaturizes the basic functions of chemical and biological laboratories to a chip as small as a few square centimeters. Through the cross of analytical chemistry, micro-electro-mechanical machining, computer, electronics, materials and biology, medicine, etc., miniaturization, automation, integration and portability from sample processing to detection are realized.

[0052] In the prior art, it is difficult to realize high-precision nucleic acid extraction and amplification by microfluidic chip. Specifically, although a microfluidic chip is provided in patent CN116371491A, there is no suitable control method to realize high-precision nucleic acid extraction and amplification.

[0053] In view of this, please refer to Figure 1 The nucleic acid processing method provided in the embodiments of the present application can solve this problem, which will be described in detail below.

[0054] The nucleic acid processing method provided in the embodiments of the present application comprises:

[0055] S1: placing the microfluidic chip 10 in an analyzer, wherein the microfluidic chip has an information extraction chamber 12 and a first liquid storage chamber 18, a second liquid storage chamber 19, a third liquid storage chamber 20 and a fourth liquid storage chamber 21 in communication with the information extraction chamber 12, a plurality of magnetic beads (the diameter of the magnetic beads can be 100-5000 nm) are contained in the information extraction chamber 12, and the first liquid storage chamber 18, the second liquid storage chamber 19, the third liquid storage chamber 20 and the fourth liquid storage chamber 21 respectively contain a lysis solution, a first washing solution, a second washing solution and an elution solution.

[0056] It should be noted that in the present embodiment, the microfluidic chip can be selected from the microfluidic chip in the prior art, which has a chip body 11 and an information extraction chamber 12, a first liquid storage chamber 18, a second liquid storage chamber 19, a third liquid storage chamber 20 and a fourth liquid storage chamber 21 arranged on the chip body, and the chip body also has a rotation center 15, and the chip body 11 can rotate around the rotation center 15.

[0057] The microfluidic chip further has a temperature control air cavity 13 arranged on the chip body 11, and the temperature control air cavity is communicated with the information extraction chamber 12 through a siphon pipe 14

[0058] S2: adding a sample into the information extraction chamber; specifically, the information extraction chamber is communicated with a sample adding hole through which the sample is dripped or injected.

[0059] S3: delivering the lysis solution in the first solution storage chamber 18 into the information extraction chamber, for example, by means of extrusion.

[0060] S4: controlling the microfluidic chip to rotate forward or reversely at a first preset speed (for example, the first preset speed is 100-6000 rpm) for a first preset time (for example, the first preset time is 1-700 s), so that the lysis solution and the sample both enter the bottom wall 121 of the information extraction chamber 12. Here, the forward rotation can be understood as clockwise rotation of the microfluidic chip, and the reverse rotation can be understood as counterclockwise rotation of the microfluidic chip. Figure 1

[0061] S5: controlling a magnetic member (for example, a magnetic steel) in the analyzer to approach the microfluidic chip, so that the magnetic beads are suspended in the information extraction chamber. Here, the process that the magnetic steel approaches the microfluidic chip can be understood as that the position of the microfluidic chip is unchanged, the magnetic steel is lowered, and the magnetic field generated by the magnetic steel acts on the magnetic beads to make the magnetic beads suspended.

[0062] It should be noted that in step S5, the outside of the magnetic beads is mixed with trehalose, and the mixing state is a dried state. Under the action of the magnetic field, the magnetic beads can be quickly separated from the sugar, so that the magnetic beads can quickly produce a grabbing action with the nucleic acid.

[0063] The method of step S5 further includes:

[0064] S501: controlling the microfluidic chip to rotate forward at an acceleration to a fourth preset speed (for example, 600 rpm) and then decelerate for a third preset time (for example, 1 s);

[0065] S502: controlling the microfluidic chip to rotate reversely at an acceleration to the fourth preset speed and then decelerate for the third preset time;

[0066] S503: repeating steps S501-S502 to make the liquid in the information extraction chamber produce a vortex.

[0067] Since the position of the magnetic steel is unchanged, the distance between the magnetic beads and the magnetic steel changes during the rotation of the microfluidic chip, and the magnetic beads move up and down. At the same time, the rotation mode of the microfluidic chip is constantly accelerated and decelerated while constantly rotating forward and reversely, so that the liquid is in a vortex, and finally the magnetic beads are dispersed in the vortex.​

[0068] The liquid in the information extraction chamber generates a vortex, which can stir the magnetic beads and disperse them in the liquid vortex. In this way, the magnetic beads can be suspended under the action of the magnetic field, and can also be away from the magnetic field without falling into other channels (the other channels here can be understood as chambers connected to the information extraction chamber), thereby reducing the loss of magnetic beads. It can complete the extraction of nucleic acids, improve the accuracy of inspection, and achieve high-precision nucleic acid extraction.

[0069] It should be noted that, in some embodiments, when the diameter of the magnetic beads is 200-5000 nm, the fourth preset speed is 200-3000 rpm.

[0070] In addition, the method of step S5 also includes: controlling the temperature of the information extraction chamber to be above 37° C., which can be understood as controlling the temperature of the information extraction chamber to be above 37° C. during the stirring process of the magnetic beads to improve the lysis efficiency.

[0071] S6: Control the rotation speed of the microfluidic chip to run at a fifth preset speed, the fifth preset speed is 100rpm-900rpm, for example, the fifth preset speed can be 100rpm or 500rpm or 900rpm, so that the magnetic beads gather toward the two opposite side walls 122 in the information extraction chamber. Generally speaking, the rotation direction of the microfluidic chip is different, and the side walls 122 where the magnetic beads gather are different. This can prevent the magnetic beads from falling into the channel opening for connecting the temperature-controlled air cavity in the information extraction chamber, thereby preventing the magnetic beads from being lost.

[0072] S7: Control the magnetic part to rise and move away from the microfluidic chip so that the magnetic beads stop floating. Specifically, the magnetic part can be made to rise by controlling the magnetic field. For example, the magnetic part and the microfluidic chip are 5 mm apart. After the magnetic beads stop floating, they fall to the bottom of the information extraction chamber and gather (the bottom here is understood to be the bottom of the information extraction chamber along the axis of rotation of the microfluidic chip).

[0073] S8: heating the temperature-controlled air cavity, causing the air in the temperature-controlled air cavity to expand and enter the information extraction chamber, thereby causing the gas in the temperature-controlled air cavity to be discharged;

[0074] S9: Refrigerating the temperature-controlled air cavity so that the temperature-controlled air cavity is in a negative pressure state, so as to draw the liquid in the information extraction chamber into the temperature-controlled air cavity, so that the magnetic beads and the nucleic acids adsorbed by the magnetic beads remain in the information extraction chamber;

[0075] S10: Add the first detergent in the second liquid storage chamber 19 into the information extraction chamber, and repeat steps S4-S9;

[0076] S11: Add the second detergent in the third liquid storage chamber 20 into the information extraction chamber, and repeat steps S4-S9;

[0077] S12: The eluent in the fourth reservoir 21 is added to the information extraction chamber, and steps S4-S7 are repeated to detach the nucleic acid from the magnetic beads.

[0078] To amplify the nucleic acid, after step S12, the nucleic acid processing method further comprises:

[0079] S13: The temperature control chamber 17 is heated so that the air in the temperature control chamber expands and enters the information extraction chamber to exhaust the gas in the temperature control chamber, wherein the microfluidic chip further has a detection chamber 16 arranged on the chip body and a temperature control chamber 17 arranged on the chip body, and it should be noted that the detection chamber 16 includes a quantification pool 161 and a detection pool 162 in communication, and the information extraction chamber, the quantification pool 161 of the detection chamber 16, and the temperature control chamber are sequentially communicated.

[0080] After the liquid (such as the eluent with nucleic acid) enters the detection chamber 16, the quantification pool 161 plays a role in quantitatively aliquoting the liquid, and then transports it to the detection pool 162 so that the detection pool 162 is just filled and no air bubbles are present. S14: The temperature control chamber is refrigerated so that the temperature control chamber 17 is in a negative pressure state to send the eluent with nucleic acid in the information extraction chamber 12 to the detection chamber 16, and the magnetic beads remain in the information extraction chamber 12. Specifically, when the eluent with nucleic acid passes through the detection chamber 16, due to the rotation of the microfluidic chip being driven, the centrifugal force can disperse the eluent with nucleic acid into different channels (here, the different channels can be understood as different quantification pools 161 of the detection chamber 16 and a channel cavity of a detection chamber adjacent to the temperature control chamber 17) of the detection chamber. Excess eluent will enter the temperature control chamber for storage. As can be seen, the temperature control chamber 17 plays the role of a pump and a waste liquid storage.

[0081] It should be noted that, Figure 1 The volume of the channel cavity of the detection chamber adjacent to the temperature control chamber 17 is larger than that of the other channels, and at the same time, the channel cavity adjacent to the temperature control chamber can function as a waste pool, and the side wall interface of the temperature control chamber and the channel cavity is closer to the rotation center.

[0082] In other embodiments, the channel cavity can be integrated with the temperature control chamber, and the side wall interface of the temperature control chamber and the channel cavity can be arranged at a middle position of the side wall of the temperature control chamber.

[0083] S15: The detection chamber 16 is heated under a preset temperature condition (for example, the temperature of the preset temperature condition is 60-95°C) to realize nucleic acid amplification.

[0084] It should be noted that in this embodiment, the preset temperature condition is a series of temperature cycles, for example: first reach 60° and maintain for 5s; then warm up to 72° and maintain for 10s; then warm up to 95° and maintain for 15s; then return to 60° and repeat in this manner.

[0085] In the embodiment, step S14 comprises:

[0086] S141: controlling refrigeration of the temperature-controlled room so that the temperature-controlled room is in a negative pressure state;

[0087] S142: in the case of a large flow channel, such as a size greater than 500 microns x 500 microns, the microfluidic chip is controlled to rotate at a speed greater than the second preset speed, so that the liquid in the detection chamber passes through the quantitative pool 161 into the detection pool 162 of the detection chamber under the action of centrifugation.

[0088] Alternatively, in the case of a small flow channel, such as a size less than 200 microns x 200 microns, the microfluidic chip is controlled to rotate at a third preset speed for a second preset time, then controlled to rotate at a second preset speed for a second preset time, and then controlled to rotate at a third preset speed for a second preset time, and so on, so that the liquid in the detection chamber passes through the quantitative pool 161 into the detection pool 162 of the detection chamber under the action of centrifugation, the third preset speed being less than the second preset speed.

[0089] The second preset time is 0-10s, the second preset speed is 600-6000rpm, and the third preset speed is 0-1000rpm.

[0090] In summary, the nucleic acid processing method can use nanoscale magnetic beads, use magnetic beads for stirring and separation of multiple samples, and realize high-precision nucleic acid extraction and amplification through control of the microfluidic chip.

[0091] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A method of nucleic acid processing, characterized by, The nucleic acid processing method comprises: S1: placing a microfluidic chip in an analyzer, wherein the microfluidic chip has an information extraction chamber and first, second, third and fourth liquid storage chambers in communication with the information extraction chamber, a plurality of magnetic beads are accommodated in the information extraction chamber, and the first, second, third and fourth liquid storage chambers contain a lysis solution, a first washing solution, a second washing solution and an elution solution respectively; S2: adding a sample to the information extraction chamber; S3: delivering the lysis solution in the first liquid storage chamber to the information extraction chamber; S4: controlling the microfluidic chip to rotate at a first preset speed for a first preset time; S5: controlling a magnetic component in the analyzer to approach the microfluidic chip to make the magnetic beads suspended in the information extraction chamber; wherein the method of step S5 further comprises: S501: controlling the microfluidic chip to move at a positive acceleration to a fourth preset speed and then decelerate for a third preset time; S502: controlling the microfluidic chip to move at a negative acceleration to a fourth preset speed and then decelerate for a third preset time; S503: repeating steps S501-S502 to generate vortex in the liquid in the information extraction chamber; S6: controlling the rotation speed of the microfluidic chip to run at a fifth preset speed to make the magnetic beads gather towards the side wall in the information extraction chamber; S7: controlling the magnetic component to rise away from the microfluidic chip to make the magnetic beads stop suspending; S8: heating a temperature control air cavity to make the air in the temperature control air cavity expand and enter the information extraction chamber, and making the gas in the temperature control air cavity exhaust, wherein the microfluidic chip further has a temperature control air cavity, and the temperature control air cavity and the information extraction chamber are in communication; S9: refrigerating the temperature control air cavity to make the temperature control air cavity in a negative pressure state to suck the liquid in the information extraction chamber into the temperature control air cavity, so that the magnetic beads and the nucleic acid adsorbed by the magnetic beads remain in the information extraction chamber; S10: adding the first washing solution in the second liquid storage chamber to the information extraction chamber, and repeating steps S4-S9; S11: adding the second washing solution in the third liquid storage chamber to the information extraction chamber, and repeating steps S4-S9; S12: adding the elution solution in the fourth liquid storage chamber to the information extraction chamber, and repeating steps S4-S7 to make the nucleic acid on the magnetic beads separate.

2. The nucleic acid treatment method according to claim 1, wherein, After step S12, the nucleic acid processing method further comprises: S13: heating a temperature control chamber to make the air in the temperature control chamber expand and enter the information extraction chamber to make the gas in the temperature control chamber exhaust, wherein the microfluidic chip further has a detection chamber and a temperature control chamber, and the information extraction chamber, the detection chamber and the temperature control chamber are in communication in sequence; S14: refrigerating the temperature control chamber to make the temperature control chamber in a negative pressure state to send the elution solution with nucleic acid in the information extraction chamber to the detection chamber; S15: heating the detection chamber under a preset temperature condition to realize nucleic acid amplification.

3. The nucleic acid treatment method according to claim 2, wherein, The step S14 comprises: S141: refrigerate the temperature-controlled room so that the temperature-controlled room is in a negative pressure state; S142: control the microfluidic chip to rotate at a second preset speed greater than the first preset speed, so that the liquid in the detection chamber enters the detection pool of the detection chamber under the action of centrifugal force; Alternatively, after controlling the microfluidic chip to rotate at a third preset speed for a second preset time, controlling the microfluidic chip to rotate at the second preset speed for the second preset time, and then controlling the chip to rotate at the third preset speed for the second preset time, and so on, so that the liquid in the detection chamber enters the detection pool of the detection chamber under the action of centrifugal force, the third preset speed being less than the second preset speed.

4. The nucleic acid treatment method according to claim 3, wherein, The second preset time is 0-10s, the second preset speed is 600-6000rpm, and the third preset speed is 0-1000rpm.

5. The nucleic acid treatment method according to claim 2, wherein The preset temperature condition is 60-95℃.

6. The nucleic acid treatment method according to claim 1, wherein The first preset speed is 100-6000rpm, and the first preset time is 1-700s.

7. The nucleic acid treatment method according to claim 1, wherein The diameter of the magnetic beads is 200-5000nm, and the fourth preset speed is 200-3000rpm.

8. The nucleic acid treatment method according to claim 1, wherein, The method of step S5 further comprises: Control the information extraction chamber to be warmed up to above 37℃.

9. The nucleic acid treatment method according to claim 1, wherein, The diameter of the magnetic beads is 100-5000nm.

10. The nucleic acid treatment method according to claim 1, wherein, The fifth preset speed is 100rpm-900rpm.

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