An integrated sampling, homogenization and centrifugation workstation for aquatic product testing

Abstract

The present invention provides a sampling homogenization centrifugal integrated workstation for aquatic product detection, including a shell, a centrifugal shaft that can be raised and lowered is rotatably connected in the shell, an umbrella-shaped turntable is fixedly installed on the centrifugal shaft, a plurality of radially arranged centrifugal boxes are rotatably connected to the circumference of the umbrella-shaped turntable, a placement box for inserting and placing a sampling tube is slidably installed in the centrifugal box, a tube pushing mechanism for pushing the sampling tube to a vertical state is installed on the centrifugal shaft, and a plurality of oscillation mechanisms for driving the placement box to oscillate radially in the centrifugal box are installed on the circumferential inner wall of the shell, and the plurality of oscillation mechanisms correspond to the plurality of placement boxes one by one. The device can adjust the centrifugal angle, can automatically complete the cell crushing, centrifugation and sampling processes of biological samples, can also automatically add buffer and gradient liquid, realize the integrated biological sample pretreatment process, greatly improve the automation of sampling detection, and improve detection efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of aquatic product detection, and in particular to a sampling, homogenization and centrifugation integrated workstation for aquatic product detection. Background Art

[0002] In the process of aquatic product testing, samples are usually taken for testing of animal meat tissue, fins, gills, internal organs, blood, body mucus, intestinal contents, etc.

[0003] After sampling, the biological sample needs to be pretreated to break up the tissue cells or cultured cells so that the intracellular substances are released into the buffer solution. This is the first step in extracting intracellular proteins. The sample is then placed in a centrifuge and rotated at high speed after adding a gradient liquid to separate substances of different densities and stratify them, thereby purifying the required substances.

[0004] For the sampling and testing process, tissue samples need to be processed in multiple steps. The processing process requires the cooperation of multiple instruments and tools, and the degree of automation is low. During the centrifugation process before testing, buffer and gradient liquid need to be added manually at different stages. After centrifugation, a sampling gun is also required to suck out the upper sample liquid, which is more troublesome, especially when there are many sampling tubes to be centrifuged. The processing time is longer, affecting the detection efficiency. Summary of the invention

[0005] In response to the above problems, the present invention provides an integrated sampling, homogenization and centrifugation workstation for aquatic product testing. The device can adjust the centrifugation angle, automatically complete the cell disruption, centrifugation and sampling processes of biological samples, and automatically add buffer and gradient liquid, thereby greatly improving the degree of automation of sampling and testing and improving detection efficiency.

[0006] To solve the above problems, the technical solution adopted by the present invention is:

[0007] A sampling and homogenization centrifugal integrated workstation for aquatic product testing comprises a shell, a centrifugal shaft that can be raised and lowered is rotatably connected in the shell, an umbrella-shaped turntable is fixedly mounted on the centrifugal shaft, a plurality of radially arranged centrifugal boxes are rotatably connected in the circumferential direction of the umbrella-shaped turntable, a placement box for inserting and placing sampling tubes is slidably mounted in the centrifugal box, a tube pushing mechanism for pushing the sampling tube to a vertical state is mounted on the centrifugal shaft, a plurality of oscillation mechanisms for driving the placement box to oscillate radially in the centrifugal box are mounted on the circumferential inner wall of the shell, a plurality of the oscillation mechanisms correspond one to one to a plurality of the placement boxes, and the A swivel is rotatably connected to the inner top of the shell, and a plurality of groups of buffer solution injection tubes, gradient solution injection tubes and sample layer pipette tubes are installed at the bottom of the swivel corresponding to the positions of a plurality of centrifuge boxes. A sealing cover is installed at the tube mouth of the sampling tube, and a cover hole is provided in the sealing cover, and an abutment assembly is installed in the cover hole. A plug hole corresponding to the cover hole is provided on the top of the centrifuge box, and the cover hole and the plug hole are used for inserting the buffer solution injection tube, the gradient solution injection tube or the sample layer pipette tube. A flip cover for placing the sampling tube is installed on the top of the shell, and a pair of ultrasonic generators are installed below the umbrella-shaped turntable in the shell.

[0008] Preferably, a first motor is fixedly mounted on the top of the shell, an output end of the first motor extends into the shell and is fixedly connected to a telescopic shaft, and a lower end of the telescopic shaft extends into the centrifugal shaft and is slidably connected thereto.

[0009] Preferably, a pair of electric push rods are fixedly mounted on the bottom of the shell, the telescopic ends of the electric push rods extend into the shell and are fixedly connected to a lifting plate, and the lower end of the centrifugal shaft is rotatably connected to the lifting plate.

[0010] Preferably, the tube pushing mechanism includes a telescopic cavity arranged in the centrifugal shaft, a vertical telescopic airbag is installed at the bottom of the telescopic cavity, a lifting block is fixedly installed at the telescopic end of the telescopic airbag, and a plurality of oblique connecting rods are rotatably connected to the circumferential side walls of the lifting block, a fixed block is fixedly connected to the bottom of the upper end of the centrifugal box, the upper end of the oblique connecting rod is rotatably connected to the corresponding fixed block, a strip opening is provided at the bottom of the umbrella-shaped turntable for the fixed block and the oblique connecting rod to pass through, a telescopic tube is rotatably connected to the inner bottom of the shell, the telescopic end of the telescopic tube is fixedly connected to the lower end of the centrifugal shaft and is connected to the telescopic airbag, the lower end of the telescopic tube extends out of the shell and is connected to a pump air pipe through a rotating joint, and an air pump connected to the pump air pipe is installed at the bottom of the shell.

[0011] Preferably, both ends of the placement box are provided with a slot for accommodating the end of the sampling tube, and a stop plate is elastically connected to the slot at the lower end via a first spring.

[0012] Preferably, the oscillation mechanism includes a push rod fixedly connected to the lower end of the placement box, the end of the push rod extends out of the centrifugal box and is rotatably connected to a metal ball that abuts against the inner wall of the shell, the upper end of the placement box is elastically installed in the centrifugal box through a second spring, the circumferential inner wall of the shell is provided with a plurality of grooves, cams that cooperate with the corresponding metal balls are rotatably connected in the grooves, an annular transmission box is fixedly installed on the circumferential side wall of the shell, and a driving assembly that can synchronously drive a plurality of cams to rotate is installed in the annular transmission box.

[0013] Preferably, the drive assembly includes a transmission shaft fixedly connected to the side wall of the cam, the ends of adjacent transmission shafts extend into an annular transmission box and are connected through a pair of transmission bevel gears, a second motor is fixedly installed on the rear side of the annular transmission box, the output end of the second motor extends into the annular transmission box and is fixed with a driving bevel gear, and the driving bevel gear is meshed with the corresponding transmission bevel gear.

[0014] Preferably, a plurality of vertical electromagnetic suction plates for magnetically attracting corresponding metal balls are embedded in the circumferential inner wall of the shell, a pair of limit plates are fixedly installed on both sides of the notch of the groove, and the upper end of the vertical electromagnetic suction plate extends to the bottom of the pair of limit plates.

[0015] Preferably, a third motor is installed on the top of the shell, a gear ring is fixed on the bottom of the rotating ring, a gear meshing with the gear ring is fixed on the output end of the third motor, and a plurality of annular tubes are fixedly installed on the inner top of the shell, and the plurality of groups of buffer solution injection tubes, gradient liquid injection tubes and sample layer aspiration tubes are respectively connected to the corresponding annular tubes through connecting tubes, and the plurality of annular tubes are externally connected to a liquid pump.

[0016] Preferably, the push-open assembly comprises a telescopic groove arranged in the sealing cover, and a wedge-shaped sealing block extending into the cover hole is elastically connected in the telescopic groove via a third spring.

[0017] The beneficial effects of the present invention are:

[0018] 1. By installing the centrifugal shaft, the umbrella-shaped turntable, multiple placement boxes and the tube pushing mechanism, the collected samples are placed in the sampling tube, the sealing cover is screwed on to seal, and the flip cover is opened to place multiple sampling tubes in the corresponding placement boxes. The two ends of the sampling tubes are respectively placed in the corresponding card slots, and the abutment plate is used to tighten them, which is convenient for loading. The first motor is started to drive the centrifugal shaft to rotate, and the umbrella-shaped turntable can be used to drive the sampling tubes in the multiple placement boxes to be centrifuged at high speed. The centrifugal angles of the multiple sampling tubes can also be adjusted according to actual needs through the tube pushing mechanism.

[0019] 2. By installing a pair of ultrasonic generators, the cells of the sample in the sampling tube can be broken before centrifugation. Ultrasonic or mechanical crushing can be used, or a combination of the two. When ultrasonic crushing is used, sterile water that covers the sampling tube is injected into the shell. The first motor drives multiple sampling tubes to rotate slowly, and the ultrasonic waves generated by the pair of ultrasonic generators are intermittently transmitted in the water body and act on the sampling tube to break the cells of the sample. After the sample is sampled, the shell injected with low-temperature sterile water can also be used as a storage container.

[0020] 3. By installing an oscillation mechanism, an electric push rod and a vertical electromagnetic suction plate, a mechanical crushing method is adopted. It is only necessary to start multiple circumferential vertical electromagnetic suction plates, and the umbrella-shaped turntable rotates slowly. When the metal ball rotates to the position of the vertical electromagnetic suction plate, it can be captured by the magnetic suction force, thereby completing the positioning process of the centrifugal box, the placement box and the sampling tube. The electric push rod is started, and the centrifugal shaft is driven to rise through the lifting plate, which can drive the umbrella-shaped turntable to rise as a whole. The metal ball moves up along the vertical electromagnetic suction plate, enters a pair of limit plates, and contacts with the corresponding cams. The driving assembly can synchronously drive multiple cams to rotate, and then the placement box is driven to radially oscillate in the centrifugal box through the metal ball, the stopper and the second spring, so that the sample in the sampling tube is radially oscillated, and the cells are mechanically crushed.

[0021] 4. By installing the push tube mechanism, the swivel, multiple groups of buffer injection tubes, gradient liquid injection tubes, sample layer aspirator tubes and push-open components, before performing the cell disruption operation, the air pump can be started first, and air can be pumped into the telescopic airbag through the pump air pipe and the telescopic tube. The telescopic airbag is inflated and extended upward, driving the lifting block to rise to the limit position, and then the centrifugal box is pushed to rotate to a vertical state through the oblique connecting rod and the fixed block, and the third motor is started. The swivel is driven to deflect through the gear and the gear ring, and the buffer injection tube is rotated to the corresponding sampling tube position. The electric push rod is started to drive the centrifugal box to move upward, so that the buffer injection tube passes through the jack and the cover hole, pushes the wedge-shaped sealing block open, and is inserted into the sampling tube. Buffer is injected into multiple sampling tubes simultaneously, so that the broken cell substances are integrated into the buffer. Gradient liquid can also be injected into multiple sampling tubes simultaneously through the gradient liquid injection tube before centrifugation, which is beneficial to stratification after centrifugation. After centrifugation, the upper layer of sample liquid can be sucked by inserting the sample layer aspirator tube, and the integrated pretreatment process is automatically completed, which greatly improves the automation degree of sampling detection and improves the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A perspective view of the present invention;

[0023] Figure 2 This is a schematic diagram of the three-dimensional structure of the umbrella-shaped turntable proposed by the present invention;

[0024] Figure 3 is a cross-sectional view of the present invention;

[0025] Figure 4 A cross-sectional view of a centrifugal box provided by the present invention;

[0026] Figure 5 A top view of the umbrella-shaped turntable proposed by the present invention;

[0027] Figure 6 This is a schematic diagram of the structure of the driving assembly proposed by the present invention;

[0028] Figure 7 A bottom view of the swivel provided by the present invention;

[0029] Figure 8 It is a schematic diagram of the oscillation state of the present invention;

[0030] Fig. 9 It is a schematic diagram of the liquid injection state of the present invention.

[0031] In the figure: 1 housing, 2 electric push rod, 3 air pump, 4 pump air pipe, 5 lifting plate, 6 telescopic tube, 7 telescopic airbag, 8 lifting block, 9 telescopic chamber, 10 oblique connecting rod, 11 ultrasonic generator, 12 centrifugal shaft, 13 telescopic shaft, 14 first motor, 15 liquid pump, 16 third motor, 17 gear, 18 flip cover, 19 annular tube, 20 gradient liquid injection tube, 21 swivel, 22 gear ring, 23 annular transmission box, 24 cam, 25 groove, 26 Limiting plate, 27 vertical electromagnetic suction plate, 28 umbrella-shaped turntable, 29 centrifugal box, 30 sampling tube, 31 strip-shaped mouth, 32 fixing block, 33 jack, 34 second spring, 35 placement box, 36 sealing cover, 37 cover hole, 38 wedge-shaped sealing block, 39 third spring, 40 push plate, 41 first spring, 42 push rod, 43 metal ball, 44 second motor, 45 transmission shaft, 46 transmission bevel gear, 47 buffer solution injection tube, 48 sample layer aspiration tube. DETAILED DESCRIPTION

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific implementation disclosed below.

[0033] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0034] Reference Figure 1-9 A sampling homogenization centrifugal integrated workstation for aquatic product detection comprises a shell 1, a flip cover 18 for placing a sampling tube 30 is installed on the top of the shell 1, a centrifugal shaft 12 that can be raised and lowered is rotatably connected in the shell 1, an umbrella-shaped turntable 28 is fixedly installed on the centrifugal shaft 12, a plurality of radially arranged centrifugal boxes 29 are rotatably connected to the circumference of the umbrella-shaped turntable 28, a placement box 35 for inserting and placing the sampling tube 30 is slidably installed in the centrifugal box 29, both ends of the placement box 35 are provided with a card slot for placing the end of the sampling tube 30, and the lower end card slot A push plate 40 is elastically connected to the shell 1 through a first spring 41, and a first motor 14 is fixedly installed on the top of the shell 1. The output end of the first motor 14 extends into the shell 1 and is fixedly connected to the telescopic shaft 13. The lower end of the telescopic shaft 13 extends into the centrifugal shaft 12 and is slidably connected thereto. The two ends of the sampling tube 30 are respectively placed in corresponding slots and are tightly pressed by the push plate 40, which makes loading convenient. When the first motor 14 is started to drive the centrifugal shaft 12 to rotate, the sampling tubes 30 in the multiple placement boxes 35 can be driven for high-speed centrifugation through the umbrella-shaped turntable 28.

[0035] The centrifugal shaft 12 is provided with a tube pushing mechanism for pushing the sampling tube 30 to a vertical state, and the tube pushing mechanism includes a telescopic chamber 9 arranged in the centrifugal shaft 12, a vertical telescopic airbag 7 is installed at the bottom of the telescopic chamber 9, a lifting block 8 is fixedly installed at the telescopic end of the telescopic airbag 7, and a plurality of oblique connecting rods 10 are rotatably connected to the circumferential side wall of the lifting block 8, a fixed block 32 is fixedly connected to the bottom of the upper end of the centrifugal box 29, and the upper end of the oblique connecting rod 10 is rotatably connected to the corresponding fixed block 32, and a strip opening 31 for the fixed block 32 and the oblique connecting rod 10 to pass through is provided at the bottom of the umbrella-shaped turntable 28, and the inner bottom of the shell 1 is rotatably connected to the telescopic tube 6, the telescopic end of the telescopic tube 6 is fixedly connected to the lower end of the centrifugal shaft 12 and is communicated with the telescopic airbag 7. The lower end of the telescopic tube 6 extends out of the shell 1 and is connected to the pump air pipe 4 through a rotating joint. The bottom of the shell 1 is equipped with an air pump 3 connected to the pump air pipe 4. Start the air pump 3, pump air into the telescopic airbag 7 through the pump air pipe 4 and the telescopic tube 6, the telescopic airbag 7 is inflated and extends upward, driving the lifting block 8 to rise, and the centrifugal box 29 is pushed to rotate through the oblique connecting rod 10 and the fixed block 32. The centrifugal angle can be adjusted, and the lifting block 8 can also be driven to rise to the limit position, pushing the centrifugal box 29 to rotate to a vertical state, and the liquid injection process is carried out.

[0036] The circumferential inner wall of the shell 1 is equipped with a plurality of oscillation mechanisms for driving the placement box 35 to oscillate radially in the centrifugal box 29. The plurality of oscillation mechanisms correspond to the plurality of placement boxes 35 one by one. The oscillation mechanism includes a push rod 42 fixedly connected to the lower end of the placement box 35. The end of the push rod 42 extends out of the centrifugal box 29 and is rotatably connected to a metal ball 43 that abuts against the inner wall of the shell 1. The upper end of the placement box 35 is elastically installed in the centrifugal box 29 through a second spring 34. The circumferential inner wall of the shell 1 is provided with a plurality of grooves 25. The grooves 25 are rotatably connected to cams 24 that cooperate with the corresponding metal balls 43. An annular transmission box 23 is fixedly installed on the circumferential side wall of the shell 1. A driving assembly that can synchronously drive the plurality of cams 24 to rotate is installed in the annular transmission box 23. The plurality of cams 24 can be synchronously driven to rotate by the driving assembly, and then the placement box 35 is driven to oscillate radially in the centrifugal box 29 through the metal ball 43, the push rod 42 and the second spring 34, so that the sample in the sampling tube 30 is radially oscillated, and the cells are mechanically broken.

[0037] Furthermore, the driving assembly includes a transmission shaft 45 fixedly connected to the side wall of the cam 24, the ends of adjacent transmission shafts 45 extend into the annular transmission box 23 and are connected through a pair of transmission bevel gears 46, a second motor 44 is fixedly installed on the rear side of the annular transmission box 23, the output end of the second motor 44 extends into the annular transmission box 23 and is fixed with a driving bevel gear, the driving bevel gear is meshed with the corresponding transmission bevel gear 46, the second motor 44 is started, and the multiple transmission shafts 45 are driven to rotate through the driving bevel gear and multiple pairs of transmission bevel gears 46, so that the multiple cams 24 can be driven to rotate synchronously.

[0038] Furthermore, a pair of electric push rods 2 are fixedly installed at the bottom of the shell 1. The telescopic ends of the electric push rods 2 extend into the shell 1 and are fixedly connected to the lifting plate 5. The lower end of the centrifugal shaft 12 is rotatably connected to the lifting plate 5. The electric push rods 2 can drive the umbrella-shaped turntable 28 to rise as a whole, and can be pushed to the oscillation position or used for the injection and aspiration process.

[0039] Furthermore, a plurality of vertical electromagnetic suction plates 27 for magnetically attracting corresponding metal balls 43 are embedded in the circumferential inner wall of the shell 1, and a pair of limit plates 26 are fixedly installed on both sides of the notch of the groove 25. The upper end of the vertical electromagnetic suction plate 27 extends to the bottom of the pair of limit plates 26, and the umbrella-shaped turntable 28 rotates slowly. When the metal ball 43 rotates to the position of the vertical electromagnetic suction plate 27, it can be captured by the magnetic attraction force, thereby completing the positioning process of the centrifuge box 29, the placement box 35 and the sampling tube 30.

[0040] The inner top of the housing 1 is rotatably connected with a swivel 21, and the bottom of the swivel 21 is equipped with a plurality of buffer injection tubes 47, gradient liquid injection tubes 20 and sample layer aspiration tubes 48 at positions corresponding to the plurality of centrifuge boxes 29. A sealing cover 36 is installed at the tube mouth of the sampling tube 30, and a cover hole 37 is provided in the sealing cover 36. A push-open assembly is installed in the cover hole 37. The push-open assembly includes a telescopic groove arranged in the sealing cover 36, and a wedge-shaped sealing block 38 extending into the cover hole 37 is elastically connected in the telescopic groove through a third spring 39. The top of the centrifuge box 29 is provided with a jack corresponding to the cover hole 37. 33, the cover hole 37 and the insertion hole 33 are used for inserting the buffer solution injection tube 47, the gradient solution injection tube 20 or the sample layer pipette 48. The buffer solution injection tube 47 passes through the insertion hole 33 and the cover hole 37, pushes open the wedge-shaped sealing block 38, and is inserted into the sampling tube 30. Buffer solution is injected into multiple sampling tubes 30 simultaneously to allow the broken cell substances to be integrated into the buffer solution. Gradient solution can also be injected into multiple sampling tubes 30 through the gradient solution injection tube 20 before centrifugation, which is beneficial for stratification after centrifugation. After centrifugation, the sample layer pipette 48 can be inserted to suck the sample liquid in the upper layer.

[0041] Furthermore, a third motor 16 is installed on the top of the shell 1, a gear ring 22 is fixed on the bottom of the rotating ring 21, and a gear 17 meshing with the gear ring 22 is fixed on the output end of the third motor 16. A plurality of annular tubes 19 are fixedly installed on the inner top of the shell 1. A plurality of groups of buffer injection tubes 47, gradient liquid injection tubes 20 and sample layer aspiration tubes 48 are respectively connected to the corresponding annular tubes 19 through connecting tubes. The plurality of annular tubes 19 are all externally connected to a liquid pump 15. When the corresponding liquid pump is started, a synchronous injection or aspiration process is realized through the annular tubes 19. The liquid pump 15 for suction can be externally connected to a filtering device to filter out the desired substance by membrane filtration.

[0042] A pair of ultrasonic generators 11 are installed in the shell 1 below the umbrella-shaped turntable 28, which can perform cell disruption on the sample in the sampling tube 30 before centrifugation. Ultrasonic disruption or mechanical disruption, or a combination of the two, can be used. Ultrasonic disruption is used to inject sterile water that covers the sampling tube 30 into the shell 1, and the first motor 14 drives the multiple sampling tubes 30 to rotate slowly, intermittently passing through the pair of ultrasonic generators 11. The generated ultrasonic waves propagate in the water and act on the sampling tube 30 to disrupt the cells of the sample.

[0043] Put the collected sample into the sampling tube 30, screw on the sealing cover 36 to seal it, open the flip cover 18, put multiple sampling tubes 30 into the corresponding placement box 35, and place the two ends of the sampling tube 30 in the corresponding card slots respectively. Press them tightly with the support plate 40, which makes loading convenient. After the sample is sampled, the shell 1 injected with low-temperature sterile water can also be used as a storage container.

[0044] During centrifugation, the sterile water in the shell 1 is discharged, and the first motor 14 is started to drive the centrifugal shaft 12 to rotate, so that the umbrella-shaped turntable 28 can drive the sampling tubes 30 in the placement box 35 to be centrifuged at high speed. The centrifugal angles of the multiple sampling tubes 30 can also be adjusted by the tube pushing mechanism according to actual needs.

[0045] Before centrifugation, the sample in the sampling tube 30 is subjected to cell disruption treatment, which can be carried out by ultrasonic disruption or mechanical disruption, or a combination of the two. When ultrasonic disruption is used, sterile water that covers the sampling tube 30 is injected into the shell 1, and the first motor 14 drives the multiple sampling tubes 30 to rotate slowly, and intermittently passes through a pair of ultrasonic generators 11. The ultrasonic waves generated propagate in the water body and act on the sampling tube 30, thereby disrupting the cells of the sample.

[0046] When mechanical crushing is adopted, it is only necessary to start multiple vertical electromagnetic suction plates 27 in the circumferential direction, and the umbrella-shaped turntable 28 rotates slowly. When the metal ball 43 rotates to the position of the vertical electromagnetic suction plate 27, it can be captured by the magnetic suction force, thereby completing the positioning process of the centrifugal box 29, the placement box 35 and the sampling tube 30. The electric push rod 2 is started, and the centrifugal shaft 12 is driven to rise through the lifting plate 5. The telescopic shaft 13 and the telescopic tube 6 are adaptively retracted, and the umbrella-shaped turntable 28 can be driven to rise as a whole. The metal ball 43 moves up along the vertical electromagnetic suction plate 27, enters a pair of limit plates 26, and contacts with the corresponding cam 24. The second motor 44 is started, and the multiple transmission shafts 45 are driven to rotate through the active bevel gear and multiple pairs of transmission bevel gears 46, so that the multiple cams 24 can be driven to rotate synchronously, and then the placement box 35 is driven to oscillate radially in the centrifugal box 29 through the metal ball 43, the stop rod 42 and the second spring 34, so that the sample in the sampling tube 30 is radially oscillated, and the cells are mechanically crushed.

[0047] Before the cell disruption operation is performed, the air pump 3 is started first, and air is pumped into the telescopic airbag 7 through the pump air pipe 4 and the telescopic tube 6. The telescopic airbag 7 is inflated and extended upward, driving the lifting block 8 to rise to the limit position, and then the centrifugal box 29 is pushed to rotate to a vertical state through the inclined connecting rod 10 and the fixed block 32, and the third motor 16 is started. The rotating ring 21 is driven to deflect through the gear 17 and the ring gear 22, and the buffer injection tube 47 is rotated to the position corresponding to the sampling tube 30. The electric push rod 2 is started, and the centrifugal box 29 can be driven to move upward, so that the buffer injection tube 47 passes through the insertion hole 33 and the cover hole 37, pushes open the wedge-shaped sealing block 38, and is inserted into the sampling tube 30. Buffer is injected into multiple sampling tubes 30 simultaneously, so that the broken cell substances are integrated into the buffer. Before centrifugation, gradient liquid can be injected into multiple sampling tubes 30 through the gradient liquid injection tube 20, which is conducive to stratification after centrifugation. After centrifugation, the sample liquid in the upper layer can be sucked by inserting the sample layer pipette 48, and the integrated pretreatment process is automatically completed.

[0048] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A sampling, homogenization and centrifugation integrated workstation for aquatic product testing, comprising a housing (1), characterized in that: A centrifugal shaft (12) that can be raised and lowered is rotatably connected in the shell (1), an umbrella-shaped turntable (28) is fixedly mounted on the centrifugal shaft (12), a plurality of radially arranged centrifugal boxes (29) are rotatably connected in the circumferential direction of the umbrella-shaped turntable (28), a placement box (35) for inserting a sampling tube (30) is slidably mounted in the centrifugal box (29), a tube pushing mechanism for pushing the sampling tube (30) to a vertical state is mounted on the centrifugal shaft (12), a plurality of oscillation mechanisms for driving the placement box (35) to oscillate radially in the centrifugal box (29) are mounted on the circumferential inner wall of the shell (1), the plurality of oscillation mechanisms corresponding to the plurality of placement boxes (35) in a one-to-one manner, a rotating ring (21) is rotatably connected to the inner top of the shell (1), and the bottom of the rotating ring (21) corresponds to the plurality of A plurality of groups of buffer injection tubes (47), gradient liquid injection tubes (20) and sample layer pipettes (48) are installed at the positions of the centrifugal boxes (29); a sealing cover (36) is installed at the tube mouth of the sampling tube (30); a cover hole (37) is provided in the sealing cover (36); an opening assembly is installed in the cover hole (37); a plug hole (33) corresponding to the cover hole (37) is provided at the top of the centrifugal box (29); the cover hole (37) and the plug hole (33) are for inserting the buffer injection tube (47), the gradient liquid injection tube (20) or the sample layer pipette (48); a flip cover (18) for inserting the sampling tube (30) is installed at the top of the shell (1); and a pair of ultrasonic generators (11) are installed in the shell (1) below the umbrella-shaped turntable (28); A first motor (14) is fixedly mounted on the top of the housing (1); an output end of the first motor (14) extends into the housing (1) and is fixedly connected to a telescopic shaft (13); a lower end of the telescopic shaft (13) extends into the centrifugal shaft (12) and is slidably connected thereto; A pair of electric push rods (2) are fixedly mounted on the bottom of the housing (1); the telescopic ends of the electric push rods (2) extend into the housing (1) and are fixedly connected to a lifting plate (5); and the lower end of the centrifugal shaft (12) is rotatably connected to the lifting plate (5); The tube pushing mechanism comprises a telescopic chamber (9) arranged in a centrifugal shaft (12), a vertical telescopic airbag (7) being installed at the bottom of the telescopic chamber (9), a lifting block (8) being fixedly installed at the telescopic end of the telescopic airbag (7), a plurality of oblique connecting rods (10) being rotatably connected to the circumferential side wall of the lifting block (8), a fixed block (32) being fixedly connected to the bottom of the upper end of the centrifugal box (29), the upper end of the oblique connecting rod (10) being rotatably connected to the corresponding fixed block (32), and the umbrella-shaped The bottom of the turntable (28) is provided with a strip opening (31) for a fixed block (32) and an oblique connecting rod (10) to pass through; the inner bottom of the housing (1) is rotatably connected to a telescopic tube (6); the telescopic end of the telescopic tube (6) is fixedly connected to the lower end of the centrifugal shaft (12) and communicates with the telescopic airbag (7); the lower end of the telescopic tube (6) extends out of the housing (1) and is connected to a pump air pipe (4) via a rotating joint; an air pump (3) connected to the pump air pipe (4) is installed at the bottom of the housing (1); The push-open assembly comprises a telescopic groove arranged in the sealing cover (36), wherein a wedge-shaped sealing block (38) extending into the cover hole (37) is elastically connected in the telescopic groove via a third spring (39).

2. The integrated sampling, homogenization and centrifugation workstation for aquatic product testing according to claim 1, characterized in that: Both ends of the placement box (35) are provided with a slot for placing the end of the sampling tube (30), and a stop plate (40) is elastically connected in the slot at the lower end via a first spring (41).

3. The integrated sampling, homogenization and centrifugation workstation for aquatic product testing according to claim 1 is characterized in that: The oscillating mechanism comprises a push rod (42) fixedly connected to the lower end of the placement box (35), the end of the push rod (42) extending out of the centrifugal box (29) and rotatably connected to a metal ball (43) that abuts against the inner wall of the shell (1), the upper end of the placement box (35) is elastically mounted in the centrifugal box (29) via a second spring (34), a plurality of grooves (25) are provided on the circumferential inner wall of the shell (1), cams (24) that cooperate with corresponding metal balls (43) are rotatably connected in the grooves (25), an annular transmission box (23) is fixedly mounted on the circumferential side wall of the shell (1), and a driving assembly that can synchronously drive the plurality of cams (24) to rotate is mounted in the annular transmission box (23).

4. The integrated sampling, homogenization and centrifugation workstation for aquatic product testing according to claim 3 is characterized in that: The drive assembly comprises a transmission shaft (45) fixedly connected to a side wall of the cam (24); the ends of adjacent transmission shafts (45) extend into an annular transmission box (23) and are transmission-connected via a pair of transmission bevel gears (46); a second motor (44) is fixedly mounted on the rear side of the annular transmission box (23); an output end of the second motor (44) extends into the annular transmission box (23) and is fixed with a driving bevel gear; the driving bevel gear meshes with a corresponding transmission bevel gear (46).

5. The integrated sampling, homogenization and centrifugation workstation for aquatic product testing according to claim 3 is characterized in that: A plurality of vertical electromagnetic suction plates (27) for magnetically attracting corresponding metal balls (43) are embedded in the circumferential inner wall of the shell (1), a pair of limit plates (26) are fixedly mounted on both sides of the notch of the groove (25), and the upper end of the vertical electromagnetic suction plate (27) extends to the bottom of the pair of limit plates (26).

6. The integrated sampling, homogenization and centrifugation workstation for aquatic product testing according to claim 1, characterized in that: A third motor (16) is installed on the top of the housing (1), a gear ring (22) is fixed on the bottom of the rotating ring (21), and a gear (17) meshing with the gear ring (22) is fixed on the output end of the third motor (16). A plurality of annular tubes (19) are fixedly installed on the inner top of the housing (1), and the plurality of groups of buffer injection tubes (47), gradient liquid injection tubes (20) and sample layer aspiration tubes (48) are respectively connected to corresponding annular tubes (19) through connecting tubes, and the plurality of annular tubes (19) are all externally connected to a liquid pump (15).

Images