Disinfection method of phalaenopsis pedicel buds

Abstract

The invention discloses a disinfection method of phalaenopsis pedicels. The disinfection method comprises the following specific steps: S1, plant collection: collecting a phalaenopsis stock plant; s2, stem bud treatment: treating the collected butterfly orchid stock plant to prepare stem buds; s3, preparing a disinfectant: preparing the disinfectant for disinfection; s4, surface treatment: performing disinfection and bud removal treatment on the surfaces of the stem buds; s5, disinfection treatment: soaking the stem buds into a disinfectant for disinfection treatment by using a culture bottle and the prepared disinfectant; s6, carrying out secondary disinfection treatment; s7, storing for later use; the step S5 of disinfection treatment comprises the following specific steps: S51, putting into a culture bottle; s52, a disinfectant is injected; s53, standing is carried out; s54, inverting the culture bottle; s55, drilling is carried out; s56, the disinfectant is completely discharged; the disinfection method of the phalaenopsis pedicel buds has the effects of guaranteeing the comprehensive disinfection of the pedicel buds, optimizing the disinfection efficiency of the pedicel buds, reducing the turnover times of the disinfectant and reducing the cost expenditure.

Description

Technical Field

[0001] The invention relates to the technical field of Phalaenopsis stem bud disinfection, and in particular to a Phalaenopsis stem bud disinfection method. Background Art

[0002] Phalaenopsis (scientific name: Phalaenopsisaphrodite) belongs to the genus Phalaenopsis of the orchid family. The main reason is that Phalaenopsis has a long growth cycle and is a single-stem aerial orchid, so it is difficult to carry out conventional division propagation and it is difficult to quickly reproduce a large number of finished products.

[0003] At present, most of the Phalaenopsis tissue culture producers in China use chlorine dioxide solution or mercuric chloride solution to disinfect Phalaenopsis flower stalks and buds. The main reason for disinfecting Phalaenopsis flower stalks and buds is to prevent diseases and ensure their healthy growth. Phalaenopsis is vulnerable to pathogens during the planting process, which can cause plant diseases and affect the quality and yield of flowers. By disinfecting the explants (including the stalks and buds), the pathogens attached to the surface of the explants can be effectively killed, the occurrence of diseases can be reduced, and the disease resistance and flower quality of Phalaenopsis can be improved.

[0004] When disinfecting Phalaenopsis buds, the disinfection of buds and culture bottles is usually done separately. In addition to making the operation process complicated, the disinfectant or buds will be in contact with the outside world for a long time due to the complicated process, causing contamination. In addition, during the unified soaking of buds, the buds are light and easy to float, which will also lead to incomplete disinfection of the buds. Summary of the invention

[0005] The invention discloses a method for disinfecting Phalaenopsis stem buds, aiming to solve the technical problems that disinfectant or stem buds are in contact with the outside world for a long time, causing pollution, and the stem buds are light and easy to float during immersion, resulting in incomplete disinfection of the stem buds.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A method for disinfecting Phalaenopsis stem buds comprises the following specific steps:

[0008] S1: Collecting plants: Collecting mother plants of Phalaenopsis;

[0009] S2: Processing stem buds: Processing the collected Phalaenopsis mother plants to make stem buds;

[0010] S3: Prepare disinfectant: prepare disinfectant for disinfection;

[0011] S4: Surface treatment: Disinfect the surface of the stem buds and remove the buds;

[0012] S5: Disinfection: Use a culture bottle and the prepared disinfectant to immerse the stem buds in the disinfectant for disinfection;

[0013] S6: Secondary disinfection: After the disinfectant and stem buds are separated, secondary disinfection is performed;

[0014] S7: storage backup;

[0015] The disinfection treatment in step S5 comprises the following specific steps:

[0016] S51: placing in culture bottle: selecting a culture bottle, opening the bottle mouth and placing the stem buds in the culture bottle for later use;

[0017] S52: Pour disinfectant into the culture bottle, and stop when the disinfectant covers the stem buds, and seal the bottle;

[0018] S53: Stand: Stand for 10 minutes;

[0019] S54: Invert the culture bottle: Invert the culture bottle and let it stand for another 10 minutes;

[0020] S55: Drilling: Use a tool to drill a small vent hole near the mouth of the culture bottle, so that the disinfectant can be discharged from the vent hole; during the discharge process, the water level in the bottle drops, and the pressure in the bottle gradually decreases, which accelerates the speed of the Phalaenopsis stem bud capillary liquid absorption, and performs flow disinfection on the vent hole;

[0021] S56: Drain the disinfectant: Open the bottle and drain the remaining disinfectant completely;

[0022] The S51 is placed in culture bottles, with 4-5 stem buds placed in each culture bottle.

[0023] By directly pouring the prepared disinfectant into the culture bottle used for cultivating stem buds, and placing multiple stem buds in the culture bottle for immersion disinfection, the turnover of the disinfectant can be reduced, the possibility of disinfectant contamination can be reduced, and the cost expenditure can be further reduced. At the same time, by flipping the static immersion process, after flipping, the disinfectant completely covers the culture bottle while also ensuring complete coverage of the stem buds, thereby achieving thorough disinfection. After drilling, the internal disinfectant flows out from the drilling position near the inverted bottle mouth. During the drainage process, the water level of the disinfectant in the bottle drops, and the pressure in the bottle gradually decreases, which can accelerate the liquid absorption speed of the capillary structure of the Phalaenopsis stem buds and perform flow disinfection on the air vents, thereby optimizing the disinfection efficiency.

[0024] In a preferred embodiment, the step S1, collecting plants, comprises the following specific steps:

[0025] S11: Select mother plants: Select healthy Phalaenopsis plants that are free of pests and diseases, and cut them off as mother plants;

[0026] S12: Wrapping and storage: Wrap the mother plant with wet wipes;

[0027] S13: Humidification: Spray a small amount of clean water on the outside of the wrapped wet wipes;

[0028] S14: Temporary storage of mother plants: stack the wrapped mother plants and place them in an aluminum insulation bag;

[0029] The S2, processing the stem buds comprises the following specific steps:

[0030] S21: Wipe off moisture: Bring the mother plant back to the laboratory, take it out and wipe off the moisture attached to the surface of the mother plant with a paper towel;

[0031] S22: Cutting: Use scissors to cut from the raised position of the mother plant node and divide it into multiple small sections for later use;

[0032] S23: Incision processing: Use a utility knife to make an oblique cut from the incision position to form an oblique angle at the incision;

[0033] In the above S3, a disinfectant is prepared, wherein the disinfectant consists of a mercuric chloride solution, sterile water and a surfactant, wherein the mass fraction of the mercuric chloride solution is 0.15-0.25%, and the mass fraction of the surfactant is 2-5%.

[0034] When collecting plants, wrap the outer surface of the mother plant with wet wipes and spray a small amount of clean water on the surface of the wet wipes to increase the moisture content of the wet wipes and the surface of the mother plant, which simultaneously has a cooling effect. Then wrap it with an aluminum insulation bag to seal the surrounding environment of the mother plant, which has the effect of locking in temperature, insulating, and moisturizing. This can extend the retention time of the mother plant and avoid affecting the survival probability due to untimely handling of the mother plant after picking.

[0035] In a preferred embodiment, the surface treatment in step S4 comprises the following specific steps:

[0036] S41: Surface disinfection: Spray alcohol on the surface of Phalaenopsis for disinfection;

[0037] S42: Air-dry once: Place in a dry and cool place to air-dry;

[0038] S43: Remove bracts: Gently remove the outer bracts of the sterilized stem buds;

[0039] The S6, secondary disinfection treatment comprises the following specific steps:

[0040] S61: Pour alcohol: Pour alcohol into the culture bottle;

[0041] S62: Shake the culture bottle: Shake the culture bottle quickly by hand to make the alcohol completely contact the inner wall of the culture bottle;

[0042] S63: Pour out the alcohol: open the mouth of the culture bottle, pour out the alcohol, and the poured out alcohol can be used for surface disinfection in S41;

[0043] S64: Secondary air drying: Take out the Phalaenopsis stem buds in the bottle and air dry them together with the culture bottle in a dry and cool place;

[0044] In S61, pour in alcohol so that the alcohol water level is maintained at about 1 / 4 of the culture bottle.

[0045] By carrying out a secondary disinfection treatment, alcohol can cover the Phalaenopsis stem buds and the inner wall of the culture bottle at the same time. After manual rapid shaking, the stem buds and the disinfectant in the culture bottle can be simultaneously flushed and cleaned by flowing alcohol. Therefore, after pouring out the alcohol, the poured liquid is a mixture of disinfectant and alcohol, which can be fully used for surface disinfection of the stem buds. In addition, since the air-drying efficiency of alcohol is much higher than that of disinfectant, after flushing with alcohol, in addition to achieving secondary disinfection, the air-drying time can also be greatly shortened, reducing the probability of contamination, and further ensuring the thoroughness of the stem bud disinfection.

[0046] In a preferred embodiment, the culture bottle comprises a bottle body, the outer wall of the bottle body is surrounded by an external thread, and the bottle body is engaged with a bottle cap through the external thread, a flow tube is inlaid on the inner wall of one side of the bottle body, and a plurality of limiting holes 2 and grooves 3 are simultaneously arranged on the outer side of the flow tube, and the plurality of limiting holes 2 are arranged around the outside of the groove 3;

[0047] A sealing sheet is laid in the groove three, and a plurality of the limiting plug holes two are respectively inserted with limiting plug rods two, and one end of the plurality of limiting plug rods two is simultaneously connected to a supporting ring two, the top inner wall of the bottle body is provided with an inner inclined surface, the top inner wall of the bottle body is provided with a groove one, and grooves two are equidistantly arranged in the groove one, and a limiting plug hole one is respectively provided at the bottom end of each of the grooves two, and a limiting plug rod one is movably inserted in each limiting plug hole one;

[0048] The top ends of the plurality of limit rods are fixedly connected to a support ring 1, and each limit rod 1 is surrounded by a spring. The bottom end of the support ring 1 is equidistantly fixedly connected to a plurality of vertical rods, and the bottom ends of the plurality of vertical rods are fixedly connected to a circular support plate. The circular support plate is equidistantly penetrated with a plurality of culture holes, and an elastic sheet is arranged in each culture hole.

[0049] By providing a culture bottle, a sealing sheet is placed in the groove three in the culture bottle so that the sealing sheet is arranged between the support ring two and the groove three, thereby the air hole can be directly drilled on the sealing sheet, and after the Phalaenopsis stem buds are transplanted, the culture bottle can be reused by replacing the sealing sheet, thereby reducing the cost expenditure; in addition, when opening the bottle cap, the support ring one and the circular support plate can be lifted up, and each stem bud can be inserted into the elastic sheet for clamping, thereby ensuring that there is a gap between each stem bud, which is convenient for full contact with the disinfectant during disinfection, and also allows to leave a suitable culture gap during the stem bud cultivation process.

[0050] As can be seen from the above, a method for disinfecting Phalaenopsis stem buds comprises the following specific steps: S1: collecting plants: collecting Phalaenopsis mother plants; S2: processing stem buds: processing the collected Phalaenopsis mother plants to make stem buds; S3: preparing disinfectant: preparing disinfectant for disinfection; S4: surface treatment: disinfecting the surface of the stem buds and removing the buds; S5: disinfection: using a culture bottle and the prepared disinfectant, immersing the stem buds in the disinfectant for disinfection; S6: secondary disinfection: separating the disinfectant and the stem buds and performing secondary disinfection; S7: storing for standby; the S5, disinfection treatment comprises the following specific steps: S51: placing in a culture bottle: selecting a culture bottle, opening the bottle mouth and placing the stem buds in the culture bottle for standby; S52: pouring disinfectant: pouring disinfectant into the culture bottle, and stopping after the disinfectant covers the stem buds, and sealing; S53: standing: standing for 10 minutes; S54: inverting the culture bottle: inverting the culture bottle and standing for 10 minutes again;

[0051] S55: drilling: using a tool to drill a small vent hole near the mouth of the culture bottle, so that the disinfectant can be discharged from the vent hole; S56: draining the disinfectant: opening the bottle mouth, and completely draining the remaining disinfectant. The method for disinfecting Phalaenopsis stem buds provided by the present invention has the technical effects of ensuring the comprehensiveness of stem bud disinfection, optimizing the efficiency of stem bud disinfection, reducing the turnover times of the disinfectant, and reducing cost expenditure. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 The present invention provides an overall flow chart of a method for disinfecting Phalaenopsis stem buds.

[0053] Figure 2 The present invention provides a flow chart of the collection of plants for the method of disinfecting Phalaenopsis stem buds.

[0054] Figure 3 The present invention provides a flow chart of treating stem buds in a method for disinfecting stem buds of Phalaenopsis.

[0055] Figure 4 The present invention provides a surface treatment flow chart of a method for disinfecting Phalaenopsis stem buds.

[0056] Figure 5 The present invention provides a disinfection treatment flow chart of a method for disinfecting Phalaenopsis stem buds.

[0057] Figure 6 The present invention provides a secondary disinfection treatment flow chart of a method for disinfecting Phalaenopsis stem buds.

[0058] Figure 7 The present invention is a schematic diagram of the overall structure of a culture bottle for a method of sterilizing Phalaenopsis stem buds.

[0059] Figure 8 A schematic diagram of the disassembled internal structure of a culture bottle of a method for disinfecting Phalaenopsis stem buds proposed in the present invention.

[0060] Fig. 9 The present invention is a schematic diagram of the disassembly of a circulation tube of a method for disinfecting Phalaenopsis stem buds.

[0061] In the attached drawings: 1. bottle cap; 2. circulation tube; 3. bottle body; 4. support ring 1; 5. vertical rod; 6. limit rod 1; 7. spring; 8. groove 1; 9. groove 2; 10. limit hole 1; 11. inner slope; 12. circular support plate; 13. culture hole; 14. elastic sheet; 15. external thread; 16. limit hole 2; 17. groove 3; 18. sealing sheet; 19. limit rod 2; 20. support ring 2. DETAILED DESCRIPTION

[0062] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0063] The method for disinfecting Phalaenopsis stem buds disclosed by the invention is mainly used in the scene of disinfecting Phalaenopsis stem buds.

[0064] Reference Figure 1 and Figure 5 A method for disinfecting Phalaenopsis stem buds comprises the following specific steps:

[0065] S1: Collecting plants: Collecting mother plants of Phalaenopsis;

[0066] S2: Processing stem buds: Processing the collected Phalaenopsis mother plants to make stem buds;

[0067] S3: Prepare disinfectant: prepare disinfectant for disinfection;

[0068] S4: Surface treatment: Disinfect the surface of the stem buds and remove the buds;

[0069] S5: Disinfection: Use a culture bottle and the prepared disinfectant to immerse the stem buds in the disinfectant for disinfection;

[0070] S6: Secondary disinfection: After the disinfectant and stem buds are separated, secondary disinfection is performed;

[0071] S7: storage backup;

[0072] S5, disinfection treatment includes the following specific steps:

[0073] S51: placing in culture bottle: selecting a culture bottle, opening the bottle mouth and placing the stem buds in the culture bottle for later use;

[0074] S52: Pour disinfectant into the culture bottle, and stop when the disinfectant covers the stem buds, and seal the bottle;

[0075] S53: Stand: Stand for 10 minutes;

[0076] S54: Invert the culture bottle: Invert the culture bottle and let it stand for another 10 minutes;

[0077] S55: Drilling: Use a tool to drill a small vent hole near the mouth of the culture bottle to allow the disinfectant to be discharged from the vent hole;

[0078] S56: drain the disinfectant: open the bottle mouth and completely drain the remaining disinfectant. By directly pouring the prepared disinfectant into the culture bottle for cultivating stem buds, and placing multiple stem buds in the culture bottle for immersion disinfection, the turnover of the disinfectant can be reduced. The same batch of disinfectant can be used to simultaneously complete the simultaneous disinfection of the stem buds and the culture bottle. This can reduce the operating process while reducing the possibility of disinfectant contamination by reducing the steps, further reducing cost expenditure. At the same time, by flipping the static immersion process, based on the small internal space of the culture bottle, a limit is provided for the flipping action of the stem bud. After flipping, the disinfectant completely covers the culture bottle while also ensuring complete coverage of the stem bud, thereby achieving thorough disinfection. After drilling, the internal disinfectant flows out from the drilling position near the inverted bottle mouth. During the drainage process, the water level of the disinfectant in the bottle drops, and the pressure in the bottle gradually decreases, which can accelerate the liquid absorption speed of the capillary structure of the Phalaenopsis stem buds and perform flow disinfection on the air vents, thereby optimizing the disinfection efficiency.

[0079] Reference Figure 5 In a preferred embodiment, S51, place in culture bottles, with 4-5 stem buds in each culture bottle.

[0080] Reference Figure 2 In a preferred embodiment, S1, collecting plants comprises the following specific steps:

[0081] S11: Select mother plants: Select healthy Phalaenopsis plants that are free of pests and diseases, and cut them off as mother plants;

[0082] S12: Wrapping and storage: Wrap the mother plant with wet wipes;

[0083] S13: Humidification: Spray a small amount of clean water on the outside of the wrapped wet wipes;

[0084] S14: Temporary storage of mother plants: Stack the wrapped mother plants and place them in an aluminum insulation bag. When collecting the plants, wrap the outer surface of the mother plants with wet wipes and spray a small amount of clean water on the surface of the wet wipes to increase the moisture content of the wet wipes and the surface of the mother plants, which will simultaneously have a cooling effect. Then wrap them in an aluminum insulation bag to seal the surrounding environment of the mother plants, which will have the effect of locking in temperature, insulating, and moisturizing. This will extend the retention time of the mother plants and avoid affecting the survival probability due to untimely handling of the mother plants after picking.

[0085] Reference Figure 3 In a preferred embodiment, S2, processing the stem buds comprises the following specific steps:

[0086] S21: Wipe off moisture: Bring the mother plant back to the laboratory, take it out and wipe off the moisture attached to the surface of the mother plant with a paper towel;

[0087] S22: Cutting: Use scissors to cut from the raised position of the mother plant node and divide it into multiple small sections for later use;

[0088] S23: Cutting process: Use a utility knife to make an oblique cut from the cutting position to form an oblique angle at the cutting position.

[0089] Reference Figure 1 In a preferred embodiment, S3, a disinfectant is prepared, wherein the disinfectant consists of a mercuric chloride solution, sterile water and a surfactant, the mass fraction of the mercuric chloride solution is 0.15-0.25%, and the mass fraction of the surfactant is 2-5%.

[0090] Reference Figure 4 In a preferred embodiment, S4, surface treatment comprises the following specific steps:

[0091] S41: Surface disinfection: Spray alcohol on the surface of Phalaenopsis for disinfection;

[0092] S42: Air-dry once: Place in a dry and cool place to air-dry;

[0093] S43: Peel off the bracts: Gently peel off the outer bracts of the disinfected stem buds.

[0094] Reference Figure 6 In a preferred embodiment, S6, the secondary disinfection treatment comprises the following specific steps:

[0095] S61: Pour alcohol: Pour alcohol into the culture bottle;

[0096] S62: Shake the culture bottle: Shake the culture bottle quickly by hand to make the alcohol completely contact the inner wall of the culture bottle;

[0097] S63: Pour out the alcohol: open the mouth of the culture bottle, pour out the alcohol, and the poured out alcohol can be used for surface disinfection in S41;

[0098] S64: Secondary air drying: Take out the Phalaenopsis stem buds in the bottle and air dry them together with the culture bottle in a dry and cool place;

[0099] S61, pour in alcohol and keep the alcohol water level at about 1 / 4 of the culture bottle, through secondary disinfection treatment, make the alcohol cover the Phalaenopsis stem buds and the inner wall of the culture bottle at the same time, after manual rapid shaking, the stem buds can be quickly rinsed with flowing alcohol, and the disinfectant adhering to the surface of the stem buds can be rinsed off and replaced with alcohol. Correspondingly, the disinfectant in the culture bottle is rinsed simultaneously, so that after pouring out the alcohol, the poured liquid is a mixture of disinfectant and alcohol, which can be completely used for surface disinfection of the stem buds. In addition, because the air-drying efficiency of alcohol is much higher than that of disinfectant, after flushing with alcohol, in addition to achieving secondary disinfection, the air-drying time can be greatly shortened, the probability of contamination can be reduced, and the thoroughness of the stem bud disinfection can be further ensured.

[0100] Reference Figure 7-9 In a preferred embodiment, the culture bottle includes a bottle body 3, the outer wall of the bottle body 3 is surrounded by an external thread 15, and the bottle body 3 is engaged with a bottle cap 1 through the external thread 15, a flow tube 2 is embedded in the inner wall of one side of the bottle body 3, and a plurality of limiting holes 16 and grooves 17 are simultaneously provided on the outer side of the flow tube 2, and the plurality of limiting holes 16 are arranged around the outside of the groove 17.

[0101] Reference Figure 7-9 In a preferred embodiment, a sealing sheet 18 is laid in the groove three 17, and a plurality of limiting plug holes 16 are respectively inserted with limiting plug rods 19, and one end of the plurality of limiting plug rods 19 is simultaneously connected to a support ring 20, an inner bevel 11 is provided on the top inner wall of the bottle body 3, a groove 8 is provided on the top inner wall of the bottle body 3, and grooves 29 are equidistantly arranged in the groove 8, a limiting plug hole 10 is respectively provided at the bottom end of each groove 29, and a limiting plug rod 6 is movably inserted in each limiting plug hole 10.

[0102] Reference Figure 7-9In a preferred embodiment, the top ends of the plurality of limiting plug rods 6 are fixedly connected to the support ring 4 at the same time, and each limiting plug rod 6 is surrounded by a spring 7, and the bottom end of the support ring 4 is fixedly connected to the plurality of vertical rods 5 at equal intervals, and the bottom ends of the plurality of vertical rods 5 are fixedly connected to the circular support plate 12 at the same time, and the circular support plate 12 is evenly penetrated with a plurality of culture holes 13, and each culture hole 13 is respectively provided with an elastic sheet 14, and the position of the support ring 20 is fixed by placing the sealing sheet 18 into the groove 3 17 in the culture bottle, and inserting the plurality of limiting plug rods 2 19 into the limiting plug hole 2 16, so that the sealing sheet 18 is placed between the support ring 20 and the groove 3 17, by The air holes can be directly drilled on the sealing sheet 18. After the Phalaenopsis stem buds are transplanted, the sealing sheet 18 can be replaced to reuse the culture bottle, thereby reducing cost expenditure. In addition, when the bottle cap 1 is opened, the support ring 4 and the circular support plate 12 can be lifted up under the rebound effect of the spring 7 and the limiting effect of the limiting plug rod 6, and each stem bud can be inserted into the elastic sheet 14 for clamping, thereby ensuring that there is a gap between each stem bud, which is convenient for full contact with the disinfectant during disinfection, and a suitable culture gap can be left during the stem bud cultivation process. The lifting structure can facilitate the insertion of the stem buds. After the bottle cap 1 is covered, the circular support plate 12 can be driven down to drive the stem buds back into the bottle body 3.

[0103] Working principle: By directly pouring the prepared disinfectant into the culture bottle for cultivating stem buds, and placing multiple stem buds in the culture bottle for immersion disinfection, the turnover of disinfectant can be reduced, and the same batch of disinfectant can be used to simultaneously complete the simultaneous disinfection of stem buds and culture bottles, thereby reducing the operation process while reducing the possibility of disinfectant contamination by reducing steps, further reducing cost expenditure, and at the same time, by flipping the static immersion process, based on the small internal space of the culture bottle, providing a limit for the flipping action of the stem buds, after flipping, the disinfectant completely covers the culture bottle while also ensuring complete coverage of the stem buds, achieving thorough disinfection, and after drilling, the internal disinfectant flows out from the drilling position near the inverted bottle mouth, the water level of the disinfectant in the bottle drops during the drainage process, and the pressure in the bottle gradually decreases, which can accelerate the liquid absorption speed of the Phalaenopsis stem bud capillary structure and perform flow disinfection on the air vents, thereby optimizing the disinfection efficiency, and at the same time, by using wet wipes to wrap the outer surface of the mother plant when collecting the plant. The surface of the wet wipes is sprayed with a small amount of clean water to increase the moisture content of the wet wipes and the surface of the mother plant, which simultaneously plays a cooling role. Subsequently, an aluminum insulation bag is used to wrap the mother plant to seal the surrounding environment of the mother plant, which plays a role in locking temperature, heat insulation, and moisturizing. This can extend the retention time of the mother plant and avoid affecting the survival probability due to untimely treatment of the mother plant after picking. Finally, through secondary disinfection, alcohol covers the Phalaenopsis stem buds and the inner wall of the culture bottle at the same time. After manual rapid shaking, the stem buds can be quickly rinsed with flowing alcohol to rinse off the disinfectant adhering to the surface of the stem buds and replace it with alcohol. Correspondingly, the disinfectant in the culture bottle is rinsed synchronously, so that after pouring out the alcohol, the poured liquid is a mixture of disinfectant and alcohol, which can be completely used for surface disinfection of the stem buds. In addition, since the air-drying efficiency of alcohol is much higher than that of the disinfectant, after alcohol flushing, in addition to achieving secondary disinfection, the air-drying time can be greatly reduced, the probability of contamination can be reduced, and the thoroughness of the stem bud disinfection can be further guaranteed.

[0104] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A method for disinfecting Phalaenopsis stem buds, characterized in that: The specific steps include: S1: Collecting plants: Collecting mother plants of Phalaenopsis; S2: Processing stem buds: Processing the collected Phalaenopsis mother plants to make stem buds; S3: Prepare disinfectant: prepare disinfectant for disinfection; S4: Surface treatment: Disinfect the surface of the stem buds and remove the buds; S5: Disinfection: Use a culture bottle and the prepared disinfectant to immerse the stem buds in the disinfectant for disinfection; S6: Secondary disinfection: After the disinfectant and stem buds are separated, secondary disinfection is performed; S7: storage backup; The disinfection treatment in step S5 comprises the following specific steps: S51: placing in culture bottle: selecting a culture bottle, opening the bottle mouth and placing the stem buds in the culture bottle for later use; S52: Pour disinfectant into the culture bottle, and stop when the disinfectant covers the stem buds, and seal the bottle; S53: Stand: Stand for 10 minutes; S54: Invert the culture bottle: Invert the culture bottle and let it stand for another 10 minutes; S55: Drilling: Use a tool to drill a small vent hole near the mouth of the culture bottle to allow the disinfectant to be discharged from the vent hole; S56: Drain the disinfectant: Open the bottle and completely drain the remaining disinfectant.

2. The method for disinfecting Phalaenopsis stem buds according to claim 1, characterized in that: The S51 is placed in culture bottles, with 4-5 stem buds placed in each culture bottle.

3. The method for disinfecting Phalaenopsis stem buds according to claim 1, characterized in that: The step S1, collecting plants, comprises the following specific steps: S11: Select mother plants: Select healthy Phalaenopsis plants that are free of pests and diseases, and cut them off as mother plants; S12: Wrapping and storage: Wrap the mother plant with wet wipes; S13: Humidification: Spray a small amount of clean water on the outside of the wrapped wet wipes; S14: Temporary storage of mother plants: stack the wrapped mother plants and place them in an aluminum insulation bag.

4. The method for disinfecting Phalaenopsis stem buds according to claim 3, characterized in that: The S2, processing the stem buds comprises the following specific steps: S21: Wipe off moisture: Bring the mother plant back to the laboratory, take it out and wipe off the moisture attached to the surface of the mother plant with a paper towel; S22: Cutting: Use scissors to cut from the raised position of the mother plant node and divide it into multiple small sections for later use; S23: Cutting process: Use a utility knife to make an oblique cut from the cutting position to form an oblique angle at the cutting position.

5. The method for disinfecting Phalaenopsis stem buds according to claim 1, characterized in that: In the above S3, a disinfectant is prepared, wherein the disinfectant consists of a mercuric chloride solution, sterile water and a surfactant, wherein the mass fraction of the mercuric chloride solution is 0.15-0.25%, and the mass fraction of the surfactant is 2-5%.

6. The method for disinfecting Phalaenopsis stem buds according to claim 1, characterized in that: The surface treatment in S4 includes the following specific steps: S41: Surface disinfection: Spray alcohol on the surface of Phalaenopsis for disinfection; S42: Air-dry once: Place in a dry and cool place to air-dry; S43: Peel off the bracts: Gently peel off the outer bracts of the disinfected stem buds.

7. The method for disinfecting Phalaenopsis stem buds according to claim 6, characterized in that: The S6, secondary disinfection treatment comprises the following specific steps: S61: Pour alcohol: Pour alcohol into the culture bottle; S62: Shake the culture bottle: Shake the culture bottle quickly by hand to make the alcohol completely contact the inner wall of the culture bottle; S63: Pour out the alcohol: open the mouth of the culture bottle, pour out the alcohol, and the poured out alcohol can be used for surface disinfection in S41; S64: Secondary air drying: Take out the Phalaenopsis stem buds in the bottle and air dry them together with the culture bottle in a dry and cool place; In S61, pour in alcohol so that the alcohol water level is maintained at about 1 / 4 of the culture bottle.

8. The method for disinfecting Phalaenopsis stem buds according to claim 1, characterized in that: The culture bottle comprises a bottle body (3), the outer wall of the bottle body (3) is surrounded by an external thread (15), and the bottle body (3) is engaged with a bottle cap (1) through the external thread (15), a flow tube (2) is embedded in the inner wall of one side of the bottle body (3), and the outer side of the flow tube (2) is simultaneously provided with a plurality of limiting holes (16) and grooves (17), and the plurality of limiting holes (16) are arranged around the outside of the grooves (17).

9. The method for disinfecting Phalaenopsis stem buds according to claim 8, characterized in that: A sealing sheet (18) is laid in the groove three (17), and a plurality of the limiting plug holes two (16) are respectively inserted with limiting plug rods two (19), and one end of the plurality of limiting plug rods two (19) is simultaneously connected to a supporting ring two (20), the top inner wall of the bottle body (3) is provided with an inner inclined surface (11), the top inner wall of the bottle body (3) is provided with a groove one (8), and grooves two (9) are equidistantly arranged in the groove one (8), and a limiting plug hole one (10) is respectively provided at the bottom end of each of the grooves two (9), and a limiting plug rod one (6) is movably inserted in each limiting plug hole one (10).

10. The method for disinfecting Phalaenopsis stem buds according to claim 9, characterized in that: The top ends of the plurality of limit rods (6) are fixedly connected to a support ring (4) at the same time, and each limit rod (6) is surrounded by a spring (7) at the outside, the bottom end of the support ring (4) is fixedly connected to a plurality of vertical rods (5) at equal intervals, and the bottom ends of the plurality of vertical rods (5) are fixedly connected to a circular support plate (12) at the same time, and the circular support plate (12) is provided with a plurality of culture holes (13) at equal intervals, and each culture hole (13) is provided with an elastic sheet (14) at the inside.

Images