Composition containing benzalkonium bromide and chlorhexidine and application thereof

Abstract

The invention discloses an application of benzalkonium bromide and chlorhexidine in prevention and treatment of plant diseases, and discloses a benzalkonium bromide and chlorhexidine compound composition which has a relatively good synergistic effect in prevention and treatment of the plant diseases and has a very good application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular relates to the application of benzalkonium bromide and chlorhexidine in preventing and controlling plant diseases, and a composition containing benzalkonium bromide and chlorhexidine and the application of the composition in preventing and controlling plant diseases. Background Art

[0002] The trunks, branches, leaves, fruits and ground in the citrus orchard are often covered with a layer of moss. This is moss disease, and its main symptoms are as follows:

[0003] 1. Moss attaches to citrus leaves and affects the normal growth of citrus plants by affecting their photosynthesis.

[0004] 2. Moss attaches to leaves or the surface of trees, creating conditions for the breeding of pests and diseases. It also blocks the tree's absorption of pesticides and fertilizers, increasing the cost of using pesticides and fertilizers. If moss spreads to the fruit, it will directly lead to a decline in fruit quality and affect the income of fruit farmers.

[0005] 3. Moss covering the ground will cause the soil to become less air permeable, hinder the penetration of water, and reduce the soil's ability to retain water and fertilizer; it will also compete with citrus for fertilizer and water, affect the growth of the root system, and cause the tree to weaken.

[0006] Benzalkonium bromide is also known as dodecyl dimethyl benzyl ammonium bromide. Benzalkonium bromide is a cationic surface active fungicide. It acts on the surface of bacteria, destroys the outer membrane structure of bacteria through the combination of positive charge and negative charge of bacteria, causes the denaturation of bacterial protein and causes the death of bacteria. It is often used as a fungicide and algaecide in industrial oil fields, and has strong fungicidal and algaecidal effects. Benzalkonium bromide CAS: 7281-04-1, molecular formula C21H38BrN, molecular weight 384.437, molecular formula is as follows:

[0007]

[0008] Chlorhexidine, also known as chlorhexidine and dichlorhexidine, has a strong broad-spectrum antibacterial and bactericidal effect. Chlorhexidine CAS: 55-56-1, molecular formula C22H30Cl2N10, molecular weight 505.447, molecular formula is as follows:

[0009]

[0010] Combining different active compounds to form a pesticide composition is currently an effective and quick way to develop and research new pesticides and prevent and treat resistant pests and diseases in agriculture. However, those skilled in the art know that after mixing different active compounds, three types of effects are usually exhibited: additive effect, synergistic effect and antagonistic effect. Among them, a formula with good compounding and synergistic effect can significantly improve the actual prevention and control effect, reduce the amount of pesticide used, and thus greatly delay the speed of the development of pest and disease resistance. It is an important means of comprehensive prevention and control of diseases, but whether the compounding of two active ingredients has a synergistic effect is unpredictable.

[0011] In the prior art, there is no report that the combination of benzalkonium bromide and chlorhexidine has a synergistic effect in preventing and controlling plant diseases. Summary of the invention

[0012] The object of the present invention is to provide a synergistic composition containing benzalkonium bromide and chlorhexidine. Compared with a single agent, the synergistic composition of the present invention can improve the control effect of plant diseases, help reduce the application dosage of pesticides, and delay the development of pathogen resistance.

[0013] To achieve the above object, the present invention adopts the following technical solutions:

[0014] The first aspect of the present invention is to provide a synergistic composition, the active ingredients of which are composed of benzalkonium bromide and chlorhexidine.

[0015] The second aspect of the present invention is to provide a pesticide formulation, comprising the synergistic composition of the present invention and a pesticide-acceptable adjuvant.

[0016] The invention also discloses the use of a composite composition whose active ingredients are benzalkonium bromide and chlorhexidine in preventing and treating plant diseases. The disease is moss disease, preferably citrus moss disease.

[0017] The present invention also provides the use of benzalkonium bromide in preventing and treating plant diseases, wherein the disease is moss disease, preferably citrus moss disease.

[0018] The present invention also provides the use of chlorhexidine in preventing and treating plant diseases, wherein the disease is moss disease, preferably citrus moss disease.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) The composite synergistic composition of the present invention exhibits a synergistic effect when benzalkonium bromide and chlorhexidine are compounded, especially within a certain mass ratio range, the synergistic effect is significant, which can improve the control effect on plants, help reduce the application dose of pesticides, and delay the development of pathogen resistance.

[0021] (2) The benzalkonium bromide and chlorhexidine in the synergistic composition of the present invention are safe, quick to take effect, long-lasting and stable in efficacy, and meet the safety requirements of pesticide preparations. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 , the compound synergistic composition and the indoor control effect of each component, Figure A is the moss suspension before treatment, and Figure B is the result after 48 hours of treatment with the agent, which are, from left to right, ethoxylated, chlorhexidine, benzalkonium bromide, the compound preparation of benzalkonium bromide and chlorhexidine prepared in Example 1, and the blank control.

[0023] Figure 2 , The images of the in vitro moss before and after the treatment in Example 1 were taken under an optical microscope. Figure A is the image of the in vitro moss before the treatment, and Figure B is the moss that died 48 hours after being treated with the agent in Example 1, showing loss of greenness.

[0024] Figure 3 , images of the in vitro moss under a scanning electron microscope before and after the treatment with Example 1. Figures A and B are images of the in vitro moss under a scanning electron microscope at different magnifications before the treatment with the agent in Example 1, and Figures C and D are images of the in vitro moss under a scanning electron microscope at different magnifications 48 hours after the treatment with the agent in Example 1.

[0025] Figure 4 , indoor semi-in vitro efficacy experiment analysis process, Figure A is an untreated citrus moss leaf, Figure B is an untreated citrus moss leaf analyzed by Image J, Figure C is a citrus moss leaf treated in Example 1 for 48 hours, and Figure D is a citrus moss leaf treated in Example 1 for 48 hours analyzed by Image J; red is the moss-covered area, and gray is the moss-free area.

[0026] Figure 5 , the efficacy of the blank control group and Example 1 after treatment with the agent, the efficacy results of the blank control group and Example 1 before and after treatment with citrus tree moss leaves, moss branches and ground moss. DETAILED DESCRIPTION

[0027] The present invention is further illustrated below through specific implementation modes and detailed descriptions of the accompanying drawings, but this does not limit the present invention and is merely an example.

[0028] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise expressly stated, throughout the specification and claims, the term "including" or its variations such as "comprising" or "including" will be understood to include the stated components without excluding other components.

[0029] In the present invention, unless otherwise specified, percentage refers to mass (weight) percentage.

[0030] In addition, in order to better illustrate the present invention, numerous specific details are given in the following detailed description.

[0031] Those skilled in the art will appreciate that the present invention can be implemented without certain specific details. In some embodiments, raw materials, methods, means, etc. that are well known to those skilled in the art are not described in detail in order to highlight the main purpose of the present invention.

[0032] Specifically, the present invention first provides a synergistic composition, wherein the active ingredients of the composition are composed of benzalkonium bromide and chlorhexidine, and the mass ratio of the benzalkonium bromide to the chlorhexidine is: 1-80:50-1.

[0033] Preferably, the mass ratio of benzalkonium bromide to chlorhexidine in the above composition is: 1-20:20-1.

[0034] The second aspect of the present invention provides a pesticide formulation, which comprises the above-mentioned composition of the present invention and a pesticide-acceptable adjuvant. Preferably, the mass of the synergistic composition accounts for 0.5-95%, preferably 1-80% of the total mass of the pesticide formulation.

[0035] Preferably, the pesticide preparation of the present invention can be prepared into suitable pesticide dosage forms, such as aqueous solution, suspension, water-dispersible granule, dispersible oil suspension, microcapsule suspension and the like.

[0036] The acceptable adjuvants for the pesticide of the present invention include one or more of dispersants, wetting agents, emulsifiers, solvents, cosolvents, antifreeze agents, stabilizers, preservatives, thickeners, binders, defoamers, wall materials, acid-adjusting media, alkali-adjusting media and solid inert carriers.

[0037] Optionally, the dispersant is selected from one or more of alkylnaphthalene sulfonates, alkylbenzene sulfonate calcium salts, fatty amine polyoxyethylene ethers, bis(alkyl)naphthalene sulfonate formaldehyde condensates, naphthalenesulfonic acid formaldehyde condensates, naphthalenesulfonic acid formaldehyde condensates sodium salts, alkylphenol polyoxyethylene ethers, arylphenol polyoxyethylene succinate sulfonates, octylphenol polyoxyethylene ether sulfates, polycarboxylates, lignin sulfonate sodium salts, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, fatty acid polyoxyethylene esters and ester polyoxyethylene ethers, preferably one or more of arylphenol polyoxyethylene succinate sulfonates, polycarboxylates and lignin sulfonate sodium salts.

[0038] Optionally, the wetting agent is selected from one or more of pull-opening powder BX, sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, sodium lauryl sulfate, and saponin powder, preferably one or more of sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, and sodium lauryl sulfate.

[0039] Optionally, the emulsifier can be one or more of a cationic surfactant, an anionic surfactant, a nonionic surfactant and a zwitterionic surfactant, and any emulsifier commonly used in liquid preparations can be used. Preferably, the emulsifier is polyoxyethylene sorbitol hexastearate, polyethylene glycol fatty acid ester, polyoxyethylene sorbitol ether beeswax derivative, calcium dodecylbenzenesulfonate, sodium succinate sulfonate, styrylphenol polyoxyethylene ether phosphate, sorbitan monooleate, sorbitan monostearate, benzylphenol polyoxyethylene ether phosphate, sorbitan trioleate, sorbitan tristearate, polyethylene glycol monostearate, sorbitan sesquioleate, glycerol monostearate, dibenzylphenol polyoxyethylene ether The invention can be selected from the group consisting of ether, sorbitan monolaurate, polyoxyethylene lauryl ether, polyoxyethylene monopalmitate, fatty alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether, diethylene glycol fatty acid ester, diethylene glycol monolaurate, alkylphenol polyoxyethylene ether, polyoxyethylene lauryl ether, sorbitan polyoxyethylene ether, polyoxyethylene sorbitan ether monooleate, styrylphenol polyoxyethylene ether, and castor oil polyoxyethylene ether, preferably one or more of sorbitan trioleate and sorbitan tristearate.

[0040] Optionally, the solvent can be one or more of methanol, ethanol, propanol, isopropanol, propylene glycol, glycerol, n-butanol, isobutanol, tert-butanol, n-pentanol, isopentanol, n-hexanol, n-heptanol, n-octanol, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol dimethyl ether, sorbitol, methylene chloride, aromatic hydrocarbon solvent oil and methyl oleate, preferably one or more of ethylene glycol and methylene chloride.

[0041] Optionally, the co-solvent may be one or more of methanol, isopropanol, dimethyl sulfoxide, N-methylpyrrolidone, sulfolane and ethyl acetate.

[0042] Optionally, the antifreeze agent may be one or more of ethylene glycol, 1,2-propylene glycol, glycerol and urea, preferably ethylene glycol.

[0043] Optionally, the binder may be one or more of starch, gypsum, glucose, polyvinyl alcohol, gum arabic and dextrin, preferably starch.

[0044] Optionally, the thickener may be one or more of magnesium aluminum silicate, xanthan gum, polyethylene glycol, polyvinyl alcohol, carboxymethyl cellulose and phenolic resin, preferably one or more of magnesium aluminum silicate and xanthan gum.

[0045] Optionally, the solid inert carrier may be one or more of clay, silica, diatomaceous earth, kaolin, attapulgite, montmorillonite, bentonite, talc and light calcium carbonate, preferably one or more of silica, diatomaceous earth and kaolin.

[0046] Optionally, the defoaming agent is selected from silicone, C 8-10 Fatty alcohol, C 10-20 Saturated fatty acids (such as capric acid) and one or more of amides, silicone oils and silicone compounds. Silicone defoamers are preferred.

[0047] Optionally, the wall material is selected from one or more of polyureas, polyurethane, melamine resin and mixed polyurea / polyamide, polyamide, urea / formaldehyde, nylon, gelatin / gum arabic, polyethylene / paraffin, preferably polyamide.

[0048] Optionally, the acid adjusting medium is selected from one or more of ammonium sulfate, ammonium nitrate, ammonium chloride, hydrochloric acid, citric acid, and acetic acid.

[0049] Optionally, the alkali adjusting medium is selected from one or more of triethylamine, aqueous ammonia, sodium hydroxide and triethanolamine.

[0050] Optionally, the preservative is selected from sodium benzoate. The stabilizer may be a substance commonly used in the art, which is well known to those skilled in the art and will not be described in detail herein.

[0051] The composition of the present invention can be provided in the form of a finished product, that is, the components in the composition are already mixed; or each component can be provided in the form of a separate preparation, and directly tank mixed when used. The third aspect of the present invention provides an application of the composition and preparation of the present invention in preventing and controlling plant diseases.

[0052] Preferably, the plants are cereal crops, fruit trees, and vegetables.

[0053] Preferably, the disease is a moss disease, and citrus moss disease is particularly preferred.

[0054] The fourth aspect of the present invention also provides the use of benzalkonium bromide in preventing and controlling plant diseases, wherein the disease is moss disease, preferably citrus moss disease.

[0055] The fifth aspect of the present invention also provides the use of chlorhexidine in preventing and controlling plant diseases, wherein the disease is moss disease, preferably citrus moss disease.

[0056] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. 98% benzalkonium bromide and 98% chlorhexidine in the present invention are both commercially available, and reagents not specifically described can be purchased from the market.

[0057] 1. Preparation Example

[0058] Example 1 Preparation of 30% Benzalkonium Bromide and Chlorhexidine Soluble Solution

[0059] The processing steps of 30% benzalkonium bromide and chlorhexidine soluble solution are as follows: ① adding a small amount of solvent into a mixing kettle, then adding benzalkonium bromide and chlorhexidine into the mixing kettle according to the metered amount, starting stirring to accelerate the dispersion and dissolution of the active ingredients, and obtaining a mixture; ② after the active ingredients are dissolved, adding the auxiliary agent thereto, and continuing stirring for 0.5 to 1 hour until the auxiliary agent and the solvent are completely mixed, and after all the components are fully mixed and dissolved, the 30% benzalkonium bromide and chlorhexidine soluble solution can be obtained.

[0060] Example 2 30% Benzalkonium Bromide and Chlorhexidine Suspension

[0061] The processing method of 30% benzalkonium bromide and chlorhexidine suspension is as follows: 1. 30wt% to 80wt% of benzalkonium bromide, chlorhexidine, dispersant, wetting agent, preservative and defoamer and water are mixed and sheared for the first time, then effective ingredients benzalkonium bromide, chlorhexidine and thickener are added to mix and shear for the second time and grind, and antifreeze agent and surplus defoamer are added to mix and stir to obtain suspension. Under the mixing step condition, each component can be ensured to be fully mixed and uniform, and the dispersion stability of the suspension can be ensured. ② In some possible implementations, 30wt% to 80wt% of a dispersant, a wetting agent, a preservative, and a defoaming agent and water are cyclically sheared and mixed at a speed of 2000 to 3000 rpm for 10 to 20 minutes at a temperature below 30° C., benzalkonium bromide, chlorhexidine and a thickener are added, and the cyclic shearing and mixing at a speed of 2000 to 3000 rpm is continued for 30 to 60 minutes, and an antifreeze agent and the remaining defoaming agent are added and mixed and stirred for 30 to 60 minutes, and the material is ground until the particle size is less than 5 μm to obtain a 30% benzalkonium bromide and chlorhexidine suspension.

[0062] Example 3 20% benzalkonium bromide and chlorhexidine water dispersible granules

[0063] Benzalkonium bromide, chlorhexidine, a dispersant, a wetting agent, a filler and a disintegrant are weighed in proportion and stirred to form a mixture, which is crushed by a jet mill, granulated by a rotary extrusion granulator, and dried and sieved to obtain 20% benzalkonium bromide and chlorhexidine water dispersible granules.

[0064] Example 4 20% Benzalkonium Bromide and Chlorhexidine Microcapsule Suspension

[0065] Accurately weigh benzalkonium bromide and chlorhexidine and place them in a container. Add solvent oil and capsule material to the system, stir evenly and use it as the oil phase for later use. Add emulsifier, dispersant, protective glue and defoamer to water, shear evenly and use it as the water phase for later use. Then slowly pour the oil phase into the water phase, and shear at high speed to obtain an aqueous emulsion. Transfer the aqueous emulsion to a three-necked round-bottom flask and continue stirring. Heat at a constant temperature, slowly drop ethylenediamine aqueous solution, continue stirring until the reaction is completed, and finally add antifreeze, thickener and preservative, stir evenly to prepare 20% benzalkonium bromide and chlorhexidine microcapsule suspension.

[0066] 2. Indoor bio-test

[0067] 1) Indoor in vitro efficacy: control effect of pesticides

[0068] 1.1 Preparation of medicine

[0069] The drugs used in the in vitro efficacy experiment were commercially available control ethionine, chlorhexidine, benzalkonium bromide, the compound preparation of benzalkonium bromide and chlorhexidine prepared in Example 1, and a blank control.

[0070] 1.2 Moss suspension preparation

[0071] Weigh 1.6 g of BG11 solid culture medium and dissolve it in 1000 mL of distilled water. Place it in an autoclave at 121°C for 20 min for wet heat sterilization.

[0072] The moss on the citrus moss leaves collected in the field was scraped off with a medicine spoon, and the suspension was prepared at a ratio of 0.1 mg of the scraped citrus moss powder to 1 mL of BG11 liquid culture medium. After the moss powder was added to the culture medium, it was allowed to stand for 10 to 20 minutes, and it was inverted from time to time during the standing process until the suspension was mixed evenly. The prepared moss suspension can be successfully prepared.

[0073] 1.3 Test methods and evaluation methods

[0074] Add 1 mL of the prepared moss suspension to each 2 mL EP tube, add 0.1 mL of each reagent to the EP tube, mix thoroughly, mark, and take photos.

[0075] Place the mixture in a light incubator for static culture, with the ratio of light culture to dark culture being 16h:8h, the temperature being 26-28°C, and the humidity being 60%-80%. Meanwhile, mix the suspension evenly. Observe the experimental phenomenon 48h after the test. The moss that died after the treatment showed loss of green color.

[0076] 1.4 Results and Analysis

[0077] The drugs used in the in vitro efficacy experiment were commercially available ethionine, chlorhexidine, benzalkonium bromide, the compound preparation of benzalkonium bromide and chlorhexidine prepared in Example 1, and a blank control. Figure 1 The results showed that both benzalkonium bromide and chlorhexidine had inhibitory effects on isolated moss, and the aqueous solution prepared by their combination was significantly better than other treatments.

[0078] The moss suspension was observed under an optical microscope and a scanning electron microscope before and after the test. The moss that died 48 hours after the treatment in Example 1 showed a loss of green color ( Figure 2 ), and the dead moss cells showed cell membrane rupture and cell collapse, which is consistent with the bactericidal mechanism of benzalkonium bromide ( Figure 3 ).

[0079] 2) Indoor semi-detached efficacy: control effect of pesticides

[0080] 2.1 Preparation of medicine

[0081] The drugs used in the semi-in vitro efficacy experiment were, in order, the commercially available control ethionine, chlorhexidine, benzalkonium bromide, the compound preparation of benzalkonium bromide and chlorhexidine prepared in Example 1, and a blank control.

[0082] 2.2 Target

[0083] Citrus leaves that were severely infected with moss were collected, placed in a petri dish, and photographed.

[0084] 2.3 Test methods and evaluation

[0085] The experimental agent was sprayed at 50, 100, and 200 mg / L respectively. The condition of the citrus leaves was checked regularly and photographed after spraying. The change from green to grayish white was considered death. Three repeated treatments were set for each group, and the test results were observed after 48 hours.

[0086] The process of semi-in vitro efficacy experiment in the laboratory is as follows Figure 4 As shown in Figure 2, the surface area of ​​leaf moss infection and survival before and after application of the solution was calculated by Image J software to obtain the inhibition rate of citrus moss, and the LC of each treatment was calculated accordingly. 50 .

[0087] During the test, the detached citrus leaves were placed in a light incubator for static culture, with the ratio of light culture to dark culture being 16h:8h, the temperature being 26-28°C, and the humidity being 60%-80%.

[0088] The data of the semi-in vitro test were used to calculate the co-toxicity coefficient (CTC) of each compound according to the Sun-Johnson method. The co-toxicity coefficient was used to evaluate the action type of the agent: a co-toxicity coefficient (CTC) ≥ 120 showed a synergistic effect; a co-toxicity coefficient (CTC) ≤ 80 showed an antagonistic effect; and a co-toxicity coefficient (CTC) 80 < 120 showed an additive effect. Calculate the LC of each treatment 50 And toxicity regression curve, and calculate the co-toxicity coefficient.

[0089]

[0090]

[0091] Data processing

[0092] SPSS software was used to analyze the data and calculate the toxicity regression line, 95% confidence interval, R 2 , LC 50 and related parameters.

[0093] 2.4 Results and Analysis

[0094] Table 1 Determination of the efficacy of three tested agents (100 mg / L) on citrus moss (48 h)

[0095] Test drug <![CDATA[LC 50 Value (mg / L)]]> Virulence regression equation <![CDATA[R 2 ]]> Relative toxicity index (TI) Ethylalicin 50.732 y=1.356x-2.132 0.9977 -- Benzalkonium bromide 40.631 y=1.203x-1.936 0.9987 100 Chlorhexidine 51.376 y=1.231x-2107 0.9273 79.086

[0096] Table 2 Toxicity test results of benzalkonium bromide and chlorhexidine on citrus moss

[0097]

[0098] As shown in Table 2, within the mass ratio of 1-20:20-1, the co-toxicity coefficient CTC value of the compound combination of benzalkonium bromide and chlorhexidine on citrus moss was greater than 120, showing a synergistic effect. This shows that the compound combination of benzalkonium bromide and chlorhexidine can improve the control effect on citrus moss.

[0099] 3. Field efficacy

[0100] Test method (refer to "Guidelines for Field Efficacy Tests of Pesticides (II) GB / T17980.98-2004: Fungicides for Control of Mango Anthracnose").

[0101] In the citrus experimental garden in Pujiang County, Chengdu City, Sichuan Province, citrus trees with uniform growth, dense plants, and widespread citrus moss disease were selected for experiment use.

[0102] The experiment set up multiple treatments, each with 3 replicates and 2 fruit trees in each replicate. During the period of moss disease, the citrus trees in each treatment were sprayed with the test agent by spraying, with the degree of evenly spraying the front and back of the citrus leaves without dripping the agent, and spraying an equal amount of clean water as a blank control.

[0103] The experiment conducted three disease surveys: the first survey was conducted before the first application of pesticides, to investigate whether the plants in the plot were generally diseased; the second survey was conducted 7 days after the first application of pesticides; and the third survey was conducted 14 days after the first application of pesticides. Each tree was surveyed in five directions: east, south, west, north, and center. Ten leaves were surveyed in each direction, and the total number of leaves surveyed and the number of diseased leaves at each level were recorded. The disease index and control effect of each treatment were calculated.

[0104] In order to further verify the inhibitory effect of the compound synergistic composition on citrus moss, branches and trunks with serious disease and the ground under the same fruit trees were selected for the same uniform spraying treatment. Figure 5 shown.

[0105] The leaf disease grading table is shown in Table 3.

[0106] Table 3 Leaf disease grading table

[0107] Disease level Leaf spot area 0 No disease spots on leaves 1 The lesion area accounts for less than 10% of the leaf area 3 The lesion area accounts for 11-25% of the leaf area 5 The lesion area accounts for 26-50% of the leaf area 7 The lesion area accounts for 51-75% of the leaf area 9 The lesion area accounts for more than 75% of the leaf area

[0108] Calculation formula

[0109]

[0110]

[0111] The experimental results are shown in Table 4.

[0112] Table 4 Benzalkonium bromide, chlorhexidine, Example 1 on the control effect of citrus moss disease

[0113]

[0114] As can be seen from Table 4, 7 days after application, the compound compositions of benzalkonium bromide, chlorhexidine and benzalkonium bromide all showed certain control effects on citrus moss, and the control effect after 14 days of application was better than that after 7 days. At 14 days after application, the compound composition of benzalkonium bromide had the best control effect, which could reach 82.01%.

[0115] In vitro and semi-in vitro tests showed that the compound composition of benzalkonium bromide and chlorhexidine had a significant synergistic effect, which was further verified in field experiments.

[0116] In summary, benzalkonium bromide and chlorhexidine showed significant synergistic effects when combined, which can improve the prevention and control effect of citrus moss disease, help reduce the application dosage of pesticides, and delay the development of pathogen resistance.

[0117] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A synergistic composition, characterized in that: The active ingredients in the composition are composed of benzalkonium bromide and chlorhexidine, and the mass ratio of the benzalkonium bromide to the chlorhexidine is 1-80:50-1.

2. The composition according to claim 1, characterized in that The mass ratio of benzalkonium bromide to chlorhexidine is: 1-20:20-1.

3. A pesticide preparation, characterized in that: The synergistic composition comprises the composition of claim 1 or 2 and an adjuvant acceptable to pesticides, wherein the mass of the synergistic composition accounts for 0.5-95%, preferably 1-80% of the total mass of the pesticide preparation.

4. The preparation according to claim 3, characterized in that Can be made into suitable dosage form.

5. The dosage form according to claim 4, characterized in that The dosage form is water-based, suspension-based, water-dispersible granules, dispersible oil suspension-based or microcapsule suspension-based.

6. Use of the composition according to any one of claims 1 to 2 and the preparation according to any one of claims 3 to 5 in preventing and controlling plant diseases.

7. The use according to claim 6, characterized in that: The plants are cereal crops, fruit trees and vegetables.

8. The use according to claim 6, characterized in that: The disease is moss disease, preferably citrus moss disease.

9. Use of benzalkonium bromide in preventing and controlling plant diseases, wherein the disease is moss disease, preferably citrus moss disease.

10. Use of chlorhexidine in preventing and controlling plant diseases, wherein the disease is moss disease, preferably citrus moss disease.

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