Sleep-soothing milk and preparation method thereof

Abstract

The invention relates to sleeping milk and a preparation method thereof. The sleeping milk is prepared from the following raw materials: raw milk, theanine, casein hydrolysate, alpha-lactalbumin, sucrose fatty acid ester, glyceryl monostearate, glyceryl distearate, buffer salt and a compound extract, the compound extract comprises a combination of an oat kernel extract, a lotus seed extract, a red date extract and a banana extract. According to the invention, the theanine, the casein hydrolysate, the alpha-lactalbumin and the composite extract are combined for use, and the oat kernel extract, the lotus seed extract, the red date extract and the banana extract are added, so that the four components can interact with one another and have mutual normal-phase influence; the product has an excellent synergistic promotion effect in the aspects of soothing the nerves and helping sleep, and can further improve the sleep-soothing effect of the product after being added into the dairy product.

Description

Technical Field

[0001] The invention belongs to the field of dairy products, and in particular relates to sleeping milk and a preparation method thereof. Background Art

[0002] Wakefulness and sleep are necessary processes for human physiological activities. Through sleep, the human body can recover its energy and physical strength to maintain a good wakefulness state. Only in the wakefulness state can the human body carry out labor and other activities. "The secret of health preservation should be sleep first. Sleep can restore essence, sleep can nourish qi, sleep can strengthen spleen and stomach, sleep can strengthen bones and tendons." About 1 / 3 of a person's life is spent in sleep. Good sleep can allow the human brain and body to rest, recuperate and recover, which is helpful for daily work and study. Scientifically improving the quality of sleep is the guarantee for people's normal work, study and life.

[0003] With the accelerated pace of life, the deepening of work competition, and heavy academic workload, many people are troubled by various psychological pressures and psychological disorders. About 30% of adults in my country have sleep disorders. Sleep disorders include mild cases such as awakening several times at night, and severe cases can be sleepless all night. The most common way to eliminate sleep disorders is to take sleeping pills, but long-term use of sleeping pills may produce tolerance and addiction, and rebound insomnia and withdrawal effects may occur after long-term use, forming a vicious circle. Therefore, people are more concerned about improving sleep quality and sleep conditions through daily diet.

[0004] The α-lactalbumin contained in milk is called the "natural sleep factor" and can regulate brain nerves to improve sleep. α-lactalbumin belongs to whey protein and is the only whey protein that can bind calcium. It is a small molecule protein and an excellent source of essential amino acids and branched-chain amino acids, of which essential amino acids account for 63.2%. The content of tryptophan is relatively rich, and tryptophan is an important factor in neural development and an important nutrient for regulating sleep, appetite and mood. Theanine is a unique amino acid that mainly exists in tea plants. It can be added to food as a functional ingredient to help regulate the nervous system and is beneficial to stress, sleep, attention and memory. Many daily foods also have the effect of calming the nerves and helping sleep. Therefore, it is of great significance to develop a nutritious dairy product that has a calming effect. Summary of the invention

[0005] In view of the deficiencies in the prior art, the object of the present invention is to provide a sleeping milk and a preparation method thereof.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a soothing milk, wherein the raw materials of the soothing milk include raw cow milk, theanine, casein enzymatic hydrolysate, α-lactalbumin, sucrose fatty acid ester, mono- and distearic glyceryl, buffer salt and composite extract;

[0008] The composite extract comprises a combination of oat kernel extract, lotus seed extract, red date extract and banana extract.

[0009] In the present invention, four components, namely theanine, casein hydrolysate, α-lactalbumin and composite extract, are used in combination. It is found that the four components can cooperate with each other and have a synergistic effect in improving the product's calming and soothing effects.

[0010] Among them, oats are rich in vitamin B6 and natural melatonin, these two natural sedatives help promote relaxation and sleep; lotus seeds, as a traditional Chinese medicinal material and food ingredient, are recognized as the "fruit of sleep", rich in various nutrients such as protein, vitamins, minerals, etc. These ingredients help nourish the body and balance the endocrine system, thereby achieving a calming and soothing effect. The lotus seed core contains nelumbinine and nuciferine, which have obvious antioxidant and anti-inflammatory effects, help relieve brain fatigue and improve sleep quality; red dates contain a substance called flavonoids, which can inhibit central nervous system excitement, have a calming and sleep-inducing effect, and have excellent improvement effects on insomnia, anxiety and other symptoms of neurasthenia; bananas are rich in magnesium, potassium, tryptophan, vitamin B6, carbohydrates and fiber, etc., which can improve sleep quality through various mechanisms.

[0011] In the present invention, after the above four components are used in combination, it is found that they have a significant synergistic effect in promoting sleep and promoting sleep.

[0012] Preferably, the mass ratio of the oat kernel extract, lotus seed extract, red date extract and banana extract is (1-5):(0.5-2):(2-6):(1-3);

[0013] The above “1-5” may be, for example, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, etc.;

[0014] The above-mentioned “0.5-2” can be, for example, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.5, 1.6, 1.8, 2, etc.;

[0015] The above-mentioned “2-6” may be, for example, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, etc.;

[0016] The above-mentioned "1-3" can be, for example, 1, 1.2, 1.5, 1.7, 2, 2.2, 2.5, 2.8, 3, etc. Other specific point values ​​within the numerical range can be selected, and they will not be described one by one here;

[0017] In the present invention, when the dosage ratio of each component in the composite extract is within the above specific range, it has a more excellent effect of calming the nerves and promoting sleep.

[0018] Preferably, the added amount of the composite extract in the sleeping milk is 0.1wt%-1wt%; for example, it can be 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, etc. Other specific point values ​​within the numerical range can be selected, which will not be described here one by one.

[0019] The oat kernel extract involved in the present invention is not limited to its specific preparation process. When it is prepared by the following process, its synergistic effect with the other three components in calming the mind and promoting sleep is more significant.

[0020] Preferably, the oat kernel extract is prepared by a preparation method comprising the following steps:

[0021] The oat kernels are dried and ground, mixed with a complex enzyme solution, subjected to microwave and ultrasonic co-extraction treatment, the treated solution is centrifuged, the precipitate is mixed with water, and the microwave and ultrasonic co-extraction process is repeated, centrifuged, the supernatant is collected and combined, and freeze-dried to obtain the oat kernel extract;

[0022] The enzymes in the complex enzyme solution include α-amylase, cellulase, alkaline protease and flavor protease.

[0023] In the present invention, it is unexpectedly found that when a composite enzyme solution containing four enzymes, namely, α-amylase, cellulase, alkaline protease and flavor protease, is used to perform enzymatic hydrolysis and extraction of key active substances in oat kernels, the combination of the four enzymes can synergistically enhance the effect and obtain a better extraction effect. The prepared oat kernel extract has high activity and excellent tranquilizing effect. In addition, in the present invention, microwave and ultrasound are used in combination to assist enzymatic hydrolysis. The synergistic treatment of the two can further improve the enzymatic hydrolysis effect and obtain an oat kernel extract with a better effect.

[0024] Preferably, the mass ratio of α-amylase, cellulase, alkaline protease and flavor protease is (1-3):(2-6):(0.1-4):(0.05-2);

[0025] The above “1-3” may be, for example, 1, 1.2, 1.5, 1.7, 2, 2.2, 2.5, 2.8, 3, etc.;

[0026] The above-mentioned “2-6” may be, for example, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, etc.;

[0027] The above-mentioned “0.1-4” can be, for example, 0.05, 0.1, 0.2, 0.4, 0.5, 0.8, 1, 1.5, 2, 2.5, 3, 3.5, 4, etc.;

[0028] The above-mentioned "0.05-2" can be, for example, 0.05, 0.1, 0.2, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.5, 1.6, 1.8, 2, etc. Other specific point values ​​within this numerical range can be selected, and they will not be elaborated here one by one.

[0029] In the present invention, when the dosage ratio of the four enzymes in the composite enzyme is within the above-mentioned specific range, the enzymatic extraction effect of oat kernels can be further improved.

[0030] Preferably, the addition amount of the complex enzyme in the complex enzyme solution is 0.1‰-0.5‰ of the mass of the oat kernel, for example, it can be 0.1‰, 0.15‰, 0.2‰, 0.25‰, 0.3‰, 0.35‰, 0.4‰, 0.45‰, 0.5‰, etc. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0031] Preferably, the ratio of the oat kernel to the complex enzyme solution is 1g:(40-80)mL, for example, it can be 1g:40mL, 1g:45mL, 1g:50mL, 1g:55mL, 1g:60mL, 1g:65mL, 1g:70mL, 1g:72mL, 1g:75mL, 1g:80mL, etc. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0032] Preferably, the power of the microwave in the co-extraction process is 200-800W, for example, 200W, 300W, 400W, 500W, 600W, 700W, 800W, etc., and the microwave time is 10-60s, for example, 10s, 20s, 30s, 40s, 50s, 60s, etc.; the power of the ultrasonic wave is 200-400W, for example, 200W, 250W, 300W, 350W, 400W, etc., and the ultrasonic time is 5-30min, for example, 5min, 7min, 10min, 12min, 15min, 20min, 22min, 25min, 30min, etc. Other specific point values ​​within the numerical range can be selected, and they will not be described one by one here.

[0033] The lotus seed extract involved in the present invention is not limited to its specific preparation process. When it is prepared by the following process, its synergistic effect with the other three components in calming the mind and promoting sleep is more significant.

[0034] Preferably, the lotus seed extract is prepared by a preparation method comprising the following steps:

[0035] The lotus seed raw material is crushed and sieved, and then mixed with anhydrous ethanol for extraction under ultrasonic conditions, and the filtrate is filtered and collected, and the filter residue is mixed with anhydrous ethanol to repeat the above extraction and filtration treatment twice, and the filtrate is combined, heated to remove alcohol, and dried to obtain the lotus seed extract.

[0036] In the present invention, the sleep-inducing active ingredients in lotus seeds are extracted under ultrasonic conditions. Compared with the conventional hot soaking extraction method, the nutrients and active ingredients in the lotus seeds can be extracted more effectively, and the obtained extract has a better sleep-inducing and tranquilizing effect.

[0037] Preferably, the ultrasonic power is 200-400W, for example 200W, 250W, 300W, 350W, 400W, etc., and the ultrasonic time is 5-30min, for example 5min, 7min, 10min, 12min, 15min, 20min, 22min, 25min, 30min, etc. Other specific point values ​​within the numerical range can be selected, and they will not be described one by one here.

[0038] The red date extract and banana extract involved in the present invention are not limited to their specific preparation process. When they are prepared by the following process, the synergistic effect of them with the other three components in calming the mind and promoting sleep is more significant.

[0039] Preferably, the red date extract and banana extract are prepared by a preparation method comprising the following steps:

[0040] The red dates or banana raw materials are freeze-dried, crushed and sieved, extracted with hot water, and the filtrate is collected by filtration. Optionally, the filter residue is subjected to the hot water extraction and filtration treatments for 1-2 times, and the filtrates are combined and dried to obtain the product.

[0041] Wherein, "optionally" means that the red date raw material or banana raw material is freeze-dried, crushed and sieved, extracted with hot water, filtered and collected, and the filtrate is dried to obtain the corresponding extract;

[0042] Or the red date raw material or banana raw material is freeze-dried, crushed and sieved, extracted with hot water, filtered and collected the filtrate, the filter residue is repeatedly subjected to the hot water extraction and filtering treatment 1-2 times, the filtrate is combined and dried to obtain the corresponding extract.

[0043] Preferably, the hot water extraction temperature is 50-80°C, for example, it can be 50°C, 52°C, 55°C, 58°C, 60°C, 62°C, 65°C, 68°C, 70°C, 75°C, 80°C, etc.; the time is 30-60min, for example, it can be 30min, 32min, 35min, 38min, 40min, 42min, 45min, 48min, 50min, 52min, 55min, 58min, 60min, etc. Other specific point values ​​within the numerical range can be selected, and they will not be described one by one here.

[0044] Preferably, the raw materials of the sleeping milk include 95-99.9 parts of raw milk, 0.01-0.5 parts of theanine, 0.01-0.3 parts of casein hydrolysate, 0.05-0.5 parts of α-lactalbumin, 0.01-0.1 parts of sucrose fatty acid ester, 0.01-0.1 parts of mono- and distearic acid glyceryl, 0.01-0.05 parts of buffer salt and 0.1-1 parts of compound extract in parts by mass;

[0045] The above “95-99.9 parts” may be, for example, 95 parts, 95.5 parts, 96 parts, 96.5 parts, 97 parts, 97.5 parts, 98 parts, 98.5 parts, 99 parts, 99.5 parts, 99.9 parts, etc.;

[0046] The above-mentioned “0.01-0.5 parts” can be, for example, 0.01 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.12 parts, 0.15 parts, 0.18 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, 0.45 parts, 0.5 parts, etc.;

[0047] The above-mentioned “0.01-0.3 parts” can be, for example, 0.01 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.12 parts, 0.15 parts, 0.18 parts, 0.2 parts, 0.22 parts, 0.25 parts, 0.28 parts, 0.3 parts, etc.;

[0048] The above-mentioned “0.05-0.5 parts” can be, for example, 0.05 parts, 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, 0.45 parts, 0.5 parts, etc.;

[0049] The above-mentioned “0.01-0.1 part” can be, for example, 0.01 part, 0.02 part, 0.03 part, 0.04 part, 0.05 part, 0.06 part, 0.07 part, 0.08 part, 0.09 part, 0.1 part, etc.;

[0050] The above-mentioned “0.01-0.05 parts” can be, for example, 0.01 parts, 0.015 parts, 0.02 parts, 0.025 parts, 0.03 parts, 0.035 parts, 0.04 parts, 0.045 parts, 0.05 parts, etc.;

[0051] The above-mentioned "0.1-1 part" can be, for example, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, etc. Other specific point values ​​within the numerical range can be selected, and they will not be described here one by one.

[0052] Preferably, the casein enzymatic hydrolysate comprises biologically active decapeptides of the αS1-casein 91-100 fragment;

[0053] Among them, the above-mentioned "bioactive decapeptide" is also called α-casozepine, which has affinity for γ-aminobutyric acid (GABA) receptors in the brain, thereby relieving stress and anxiety, relaxing people, and inducing deep sleep and improving sleep quality.

[0054] Preferably, the buffer salt is selected from any one of sodium hexametaphosphate, disodium hydrogen phosphate or sodium bicarbonate, or a combination of at least two thereof.

[0055] In a second aspect, the present invention provides a sleeping milk according to the first aspect, wherein a method for preparing the sleeping milk comprises the following steps:

[0056] Theanine, buffer salt and raw milk are mixed evenly, and then mixed with casein hydrolysate, α-lactalbumin, sucrose fatty acid ester, mono- and distearic acid glycerol and composite extracts, and then homogenized. The homogenized mixture is sterilized to obtain the sleeping milk.

[0057] Preferably, the mixing temperature is 45-55°C, for example, it can be 45°C, 46°C, 47°C, 48°C, 49°C, 50°C, 51°C, 52°C, 53°C, 54°C, 55°C, etc.; the time is 8-15min, for example, it can be 8min, 9min, 10min, 11min, 12min, 13min, 14min, 15min, etc., and other specific point values ​​within the numerical range can be selected, which will not be elaborated here.

[0058] Preferably, the mixing temperature is 60-75°C, for example, it can be 60°C, 61°C, 62°C, 63°C, 64°C, 65°C, 66°C, 68°C, 70°C, 72°C, 75°C, etc.; the time is 10-25min, for example, it can be 10min, 12min, 15min, 18min, 20min, 22min, 24min, 25min, etc., and other specific point values ​​within the numerical range can be selected, which will not be described here one by one.

[0059] Preferably, the homogenization pressure is 15-30MPa, for example, it can be 15MPa, 16MPa, 17MPa, 18MPa, 19MPa, 20MPa, 22MPa, 25MPa, 28MPa, 30MPa, etc.; the temperature is 65-80℃, for example, it can be 65℃, 66℃, 67℃, 68℃, 70℃, 72℃, 75℃, 78℃, 80℃, etc.; the time is 1-6min, for example, it can be 1min, 1.5min, 2min, 2.5min, 3min, 3.5min, 4min, 4.5min, 5min, 5.5min, 6min, etc., and other specific point values ​​within the numerical range can be selected, which will not be repeated here.

[0060] Compared with the prior art, the present invention has the following beneficial effects:

[0061] In the present invention, four components, namely theanine, casein hydrolysate, α-lactalbumin and composite extract, are used in combination, and four specific components, namely oat kernel extract, lotus seed extract, red date extract and banana extract, are added. It is found that the four components can interact with each other and have a positive influence on each other, and have an excellent synergistic promoting effect in terms of calming the nerves and promoting sleep. Adding them to dairy products can further enhance the sleeping effect of the product. DETAILED DESCRIPTION

[0062] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0063] The raw cow milk involved below comes from Bright Emulsion Co., Ltd.; theanine is a product from Shanghai Jiayi Biotechnology Co., Ltd.; casein enzymatic hydrolysate is purchased from Guangdong Huapeptide Biotechnology Co., Ltd.; α-lactalbumin is a product from Shanghai Nuoshen Food Trading Co., Ltd.; sucrose fatty acid ester is a product model SE-1170 from Hangzhou Ruilin Chemical Co., Ltd.; α-amylase, cellulase, alkaline protease and flavor protease are all purchased from Shanghai McLean Biochemical Technology Co., Ltd., and the product models are A800731, C758963, P750258, and F768979, respectively.

[0064] Preparation Example 1

[0065] This preparation example provides an oat kernel extract, which is prepared by the following method:

[0066] (1) The oat kernels were freeze-dried and ground, and then mixed with a composite enzyme and water, wherein the amount of the composite enzyme added was 0.5‰ of the mass of the oat kernels, and the solid-liquid ratio of the oat kernels to water was 1 g:55 mL, wherein the composite enzyme consisted of α-amylase, cellulase, alkaline protease and flavor protease in a mass ratio of 2:4:2.5:1, and microwave-ultrasound-assisted enzymatic hydrolysis and extraction was performed at a constant temperature of 55°C, wherein the microwave conditions were 400 W, 30 s, and the ultrasonic conditions were 300 W, 20 min;

[0067] (2) After the treated liquid is centrifuged, the supernatant is collected, the precipitate is mixed with water, the microwave ultrasonic extraction process in step (1) and the centrifugation step in step (2) are repeated once, the supernatant is collected and combined, and freeze-dried to obtain the oat kernel extract.

[0068] This preparation example also provides a lotus seed extract, and its preparation method is as follows:

[0069] The lotus seed raw material is crushed and sieved, and then mixed with anhydrous ethanol at a solid-liquid ratio of 1g:20mL. The extraction is carried out under ultrasonic conditions, and the ultrasonic conditions are: temperature of 60°C, 300W, and 18min. The extract is filtered and the filtrate is collected. The filter residue is mixed with anhydrous ethanol and the above extraction and filtration treatments are repeated twice. The filtrate is combined, heated to remove alcohol, concentrated and freeze-dried to obtain the lotus seed extract.

[0070] This embodiment also provides a red date extract and a banana extract, which are prepared by the following method:

[0071] The red date raw material and the banana raw material were freeze-dried and then crushed and sieved. After hot water extraction, the solid-liquid ratio was 1g:10mL, the extraction temperature was 65°C, and the time was 45min. The extract was filtered and the filtrate was collected. The filter residue was subjected to the hot water extraction and filtration treatments for one time. The filtrates were combined, concentrated and dried to obtain red date extract and banana extract.

[0072] Preparation Example 2

[0073] This preparation example provides an oat kernel extract, which is prepared by the following method:

[0074] (1) The oat kernels were freeze-dried and ground, and then mixed with a composite enzyme and water, wherein the amount of the composite enzyme added was 0.15‰ of the mass of the oat kernels, and the solid-liquid ratio of the oat kernels to water was 1 g:40 mL, wherein the composite enzyme consisted of α-amylase, cellulase, alkaline protease and flavor protease in a mass ratio of 1:5:0.2:1.8, and microwave-ultrasound-assisted enzymatic hydrolysis and extraction was performed at a constant temperature of 50°C, wherein the microwave conditions were 700 W, 12 s, and the ultrasonic conditions were 200 W, 30 min;

[0075] (2) After the treated liquid is centrifuged, the supernatant is collected, the precipitate is mixed with water, the microwave ultrasonic extraction process in step (1) and the centrifugation step in step (2) are repeated once, the supernatant is collected and combined, and freeze-dried to obtain the oat kernel extract.

[0076] This preparation example also provides a lotus seed extract, and its preparation method is as follows:

[0077] The lotus seed raw material is crushed and sieved, and then mixed with anhydrous ethanol at a solid-liquid ratio of 1g:30mL. The extraction is carried out under ultrasonic conditions, and the ultrasonic conditions are: temperature of 50°C, 230W, 25min, the extract is filtered to collect the filtrate, the filter residue is mixed with anhydrous ethanol, and the above extraction and filtering treatments are repeated twice, the filtrate is combined, heated to remove alcohol, concentrated and freeze-dried, and the lotus seed extract is obtained.

[0078] This embodiment also provides a red date extract and a banana extract, which are prepared by the following method:

[0079] The red date raw material and the banana raw material were freeze-dried and then crushed and sieved. After hot water extraction, the solid-liquid ratio was 1g:20mL, the extraction temperature was 55°C, and the time was 52min. The extract was filtered and the filtrate was collected. The filter residue was subjected to the above hot water extraction and filtration treatment once. The filtrate was combined, concentrated and dried to obtain red date extract and banana extract.

[0080] Preparation Example 3

[0081] This preparation example provides an oat kernel extract, which is prepared by the following method:

[0082] (1) The oat kernels were freeze-dried and ground, and then mixed with a composite enzyme and water, wherein the amount of the composite enzyme added was 0.3‰ of the mass of the oat kernels, and the solid-liquid ratio of the oat kernels to water was 1 g:70 mL, wherein the composite enzyme consisted of α-amylase, cellulase, alkaline protease and flavor protease in a mass ratio of 3:2:4:0.2, and microwave ultrasonic-assisted enzymatic hydrolysis and extraction was performed at a constant temperature of 58°C, wherein the microwave conditions were 280 W, 40 s, and the ultrasonic conditions were 400 W, 5 min;

[0083] (2) After the treated liquid is centrifuged, the supernatant is collected, the precipitate is mixed with water, the microwave ultrasonic extraction process in step (1) and the centrifugation step in step (2) are repeated once, the supernatant is collected and combined, and freeze-dried to obtain the oat kernel extract.

[0084] This preparation example also provides a lotus seed extract, and its preparation method is as follows:

[0085] The lotus seed raw material is crushed and sieved, and then mixed with anhydrous ethanol at a solid-liquid ratio of 1g:15mL. The extraction is carried out under ultrasonic conditions, and the ultrasonic conditions are: temperature of 50°C, 380W, 8min, the extract is filtered to collect the filtrate, the filter residue is mixed with anhydrous ethanol, and the above extraction and filtration treatment are repeated twice, the filtrate is combined, heated to remove alcohol, concentrated and freeze-dried, and the lotus seed extract is obtained.

[0086] This embodiment also provides a red date extract and a banana extract, which are prepared by the following method:

[0087] The red date raw material and the banana raw material were freeze-dried and then crushed and sieved. After hot water extraction, the solid-liquid ratio was 1g:25mL, the extraction temperature was 60°C, and the time was 50min. The extract was filtered and the filtrate was collected. The filter residue was subjected to the hot water extraction and filtration treatments for one time. The filtrate was combined, concentrated and dried to obtain a red date extract and a banana extract.

[0088] Preparation Example 4

[0089] This preparation example provides an oat kernel extract, which uses the freeze-dried and ground oat kernels in Preparation Example 1. The preparation method thereof differs from that in Preparation Example 1 only in the enzyme composition in the complex enzyme, that is, α-amylase is not included, and the reduced amount thereof is proportionally distributed to the three enzymes of cellulase, alkaline protease and flavor protease. Other processing processes and related parameters are referred to Preparation Example 1.

[0090] Preparation Example 5

[0091] This preparation example provides an oat kernel extract, which uses the freeze-dried and ground oat kernels in Preparation Example 1. The preparation method thereof differs from that in Preparation Example 1 only in the enzyme composition in the complex enzyme, that is, cellulase is not contained, and the reduced amount thereof is proportionally distributed to three enzymes: α-amylase, alkaline protease and flavor protease. Other processing processes and related parameters are referred to Preparation Example 1.

[0092] Preparation Example 6

[0093] This preparation example provides an oat kernel extract, which uses the freeze-dried and ground oat kernels in Preparation Example 1. The preparation method thereof differs from that in Preparation Example 1 only in the enzyme composition in the complex enzyme, that is, alkaline protease is not contained, and the reduced amount thereof is proportionally distributed to three enzymes: α-amylase, cellulase and flavor protease. Other processing processes and related parameters are referred to Preparation Example 1.

[0094] Preparation Example 7

[0095] This preparation example provides an oat kernel extract, which uses the freeze-dried and ground oat kernels in Preparation Example 1. The preparation method thereof is different from that in Preparation Example 1 only in the enzyme composition in the composite enzyme, that is, flavor protease is not contained, and its reduced portion is proportionally distributed to the three enzymes of α-amylase, cellulase and alkaline protease. Other processing processes and related parameters are referred to Preparation Example 1.

[0096] Preparation Example 8

[0097] This preparation example provides an oat kernel extract, which uses a mixture of freeze-dried and ground oat kernels, a complex enzyme and water in Preparation Example 1. The preparation method is different from that in Preparation Example 1 only in that the extraction process in step (1) is carried out under static conditions for 30 minutes; then the obtained treated liquid is subjected to subsequent (2) to obtain an oat kernel extract.

[0098] Preparation Example 9

[0099] This preparation example provides a lotus seed extract, which is prepared by the following method:

[0100] The lotus seed raw material was crushed and sieved, mixed with water at a solid-liquid ratio of 1g:15mL, heated to reflux for extraction for 2.5h, and the filtrate after extraction was filtered with filter paper, concentrated and freeze-dried to obtain the lotus seed extract.

[0101] Example 1

[0102] This embodiment provides a sleeping milk, and its formula is as follows (by mass):

[0103] 99.18 parts of raw milk, 0.02 parts of theanine, 0.06 parts of casein hydrolysate, 0.2 parts of alpha-lactalbumin, 0.02 parts of sucrose fatty acid ester, 0.01 parts of mono- and distearic acid glyceryl, 0.005 parts of sodium hexametaphosphate, 0.005 parts of sodium bicarbonate and 0.5 parts of a composite extract, wherein the composite extract is composed of an oat kernel extract (Preparation Example 1), a lotus seed extract (Preparation Example 1), a red date extract (Preparation Example 1) and a banana extract (Preparation Example 1) in a mass ratio of 3:1:4:2.

[0104] Its preparation method is:

[0105] (1) The raw milk in a mixing tank is heated to 55° C., theanine, sodium hexametaphosphate, and sodium bicarbonate are added, and the mixture is stirred evenly. The mixing tank is kept warm and stirred for 8 minutes to obtain a liquid A;

[0106] (2) Weigh casein hydrolysate, α-lactalbumin, sucrose fatty acid ester, mono- and distearic glyceryl, and composite extract according to weight ratio and add them to liquid B. Stir at 65° C. for 20 min to mix well to obtain liquid C.

[0107] (3) The liquid D is homogenized at 18 MPa and 75°C.

[0108] (4) The homogenized liquid is pasteurized at 85°C for 15 seconds and then rapidly cooled to 5°C.

[0109] (5) After the product is cooled, it is sent to an aseptic filling machine for filling.

[0110] Example 2

[0111] This embodiment provides a sleeping milk, and its formula is as follows (by mass):

[0112] 97.81 parts of raw milk, 0.2 parts of theanine, 0.3 parts of casein hydrolysate, 0.5 parts of alpha-lactalbumin, 0.1 parts of sucrose fatty acid ester, 0.05 parts of mono- and distearic acid glyceryl, 0.02 parts of disodium hydrogen phosphate, 0.002 parts of sodium bicarbonate and 1 part of a composite extract, wherein the composite extract is composed of an oat kernel extract (Preparation Example 2), a lotus seed extract (Preparation Example 2), a red date extract (Preparation Example 2) and a banana extract (Preparation Example 2) in a mass ratio of 5:0.5:6:1.

[0113] Its preparation method is:

[0114] (1) The raw milk in a mixing tank is heated to 45° C., theanine, disodium hydrogen phosphate, and sodium bicarbonate are added, and the mixture is stirred evenly. The mixing tank is kept warm and stirred for 15 minutes to obtain a feed liquid A;

[0115] (2) Weigh casein hydrolysate, α-lactalbumin, sucrose fatty acid ester, mono- and distearic glycerol, and composite extract according to weight ratio and add them to liquid B. Stir at 70° C. for 15 min to mix well to obtain liquid C.

[0116] (3) The liquid D is homogenized at 25 MPa and 70°C.

[0117] (4) The homogenized liquid is pasteurized at 83°C for 18 seconds and then rapidly cooled to 4°C.

[0118] (5) After the product is cooled, it is sent to an aseptic filling machine for filling.

[0119] Example 3

[0120] This embodiment provides a sleeping milk, and its formula is as follows (by mass):

[0121] 99.545 parts of raw milk, 0.01 parts of theanine, 0.05 parts of casein hydrolysate, 0.15 parts of alpha-lactalbumin, 0.015 parts of sucrose fatty acid ester, 0.015 parts of mono- and distearic acid glyceryl, 0.01 parts of disodium hydrogen phosphate, 0.005 parts of sodium hexametaphosphate and 0.2 parts of a composite extract, wherein the composite extract is composed of an oat kernel extract (Preparation Example 3), a lotus seed extract (Preparation Example 3), a red date extract (Preparation Example 3) and a banana extract (Preparation Example 3) in a mass ratio of 1:2:2:3.

[0122] Its preparation method is:

[0123] (1) The raw milk in a mixing tank is heated to 50° C., theanine, disodium hydrogen phosphate, and sodium hexametaphosphate are added, and the mixture is stirred evenly. The mixing tank is kept warm and stirred for 10 minutes to obtain a feed liquid A;

[0124] (2) Weigh casein hydrolysate, α-lactalbumin, sucrose fatty acid ester, mono- and distearic glycerol, and composite extract according to weight ratio and add them to liquid B. Stir at 75° C. for 10 min to mix well to obtain liquid C.

[0125] (3) The liquid D was homogenized at 28 MPa and 68°C.

[0126] (4) The homogenized liquid is pasteurized at 87° C. for 12 seconds and then rapidly cooled to 6° C.

[0127] (5) After the product is cooled, it is sent to an aseptic filling machine for filling.

[0128] Example 4

[0129] This embodiment provides a sleeping milk, which is different from Example 1 only in the formula. The difference is that the oat kernel extract (Preparation Example 1) used is replaced by an equal amount of oat kernel extract (Preparation Example 4). The preparation method refers to Example 1.

[0130] Example 5

[0131] This embodiment provides a sleeping milk, which is different from Example 1 only in the formula. The difference is that the oat kernel extract (Preparation Example 1) used is replaced by an equal amount of oat kernel extract (Preparation Example 5). The preparation method refers to Example 1.

[0132] Example 6

[0133] This embodiment provides a sleeping milk, which is different from Example 1 only in the formula. The difference is that the oat kernel extract (Preparation Example 1) used is replaced by an equal amount of oat kernel extract (Preparation Example 6). The preparation method refers to Example 1.

[0134] Example 7

[0135] This embodiment provides a sleeping milk, which is different from Example 1 only in the formula. The difference is that the oat kernel extract (Preparation Example 1) used is replaced by an equal amount of oat kernel extract (Preparation Example 7). The preparation method refers to Example 1.

[0136] Example 8

[0137] This embodiment provides a sleeping milk, which is different from Example 1 only in the formula. The difference is that the oat kernel extract (Preparation Example 1) used is replaced by an equal amount of oat kernel extract (Preparation Example 8). The preparation method refers to Example 1.

[0138] Example 9

[0139] This embodiment provides a sleeping milk, which is different from Example 1 only in the formula. The difference is that the lotus seed extract (Preparation Example 1) used is replaced by an equal amount of lotus seed extract (Preparation Example 9). The preparation method refers to Example 1.

[0140] Comparative Example 1

[0141] This comparative example provides a sleeping milk, which is different from Example 1 only in the formula, that is, it does not contain oat kernel extract, and the reduced amount thereof is proportionally distributed among the three components of lotus seed extract, red date extract and banana extract.

[0142] Comparative Example 2

[0143] This comparative example provides a sleeping milk, which is different from Example 1 only in the formula, that is, it does not contain lotus seed extract, and its reduced amount is proportionally distributed to the three components of oat kernel extract, red date extract and banana extract.

[0144] Comparative Example 3

[0145] This comparative example provides a sleeping milk, which is different from Example 1 only in the formula, that is, it does not contain red date extract, and the reduced amount thereof is proportionally distributed among the three components of oat kernel extract, lotus seed extract and banana extract.

[0146] Comparative Example 4

[0147] This comparative example provides a sleeping milk, which is different from Example 1 only in the formula, that is, it does not contain banana extract, and the reduced amount of banana extract is proportionally distributed to the three components of oat kernel extract, lotus seed extract and red date extract.

[0148] Test Example 1

[0149] Sleep improvement effect test in mice

[0150] (1) Animal grouping and modeling

[0151] All mice (weight 20 ± 3 g) were free of specific pathogens. Healthy female SPF-grade Kunming mice were provided by the Shanghai Laboratory Animal Center and were given regular pelleted rodent diet and purified water. All procedures involving mice were in accordance with the guidelines provided by the Shanghai Laboratory Animal Care and Animal Experimentation Center (license number 2022122002).

[0152] A total of 90 mice (6 mice / group) were randomly selected, and 84 mice were used to construct an insomnia model using the modified horizontal turntable sleep deprivation method. Specifically, an acrylic plastic plate was used to construct a sleep deprivation box with a length of 110 cm, a width of 60 cm, and a height of 40 cm. With a longitudinal distance of 10 cm and a horizontal distance of 13 cm as the standard, 15 cylindrical platforms with a height of 8 cm and a diameter of 6.5 cm were fixed at the bottom, and 23-25°C water was injected to 1 cm below the platform to maintain the water temperature. After 7 days of adaptive training of the mice, the modeling mice were trained to stand and placed on the cylindrical platform. When the mice were about to fall asleep, they were awakened by their muscles relaxing and their heads touching the water or falling into the water, so that they were forced to stay awake and stand. During the 2-week modeling period, the mice rested once in the morning and evening, each time for 30 minutes.

[0153] 84 insomnia model mice were randomly divided into 14 groups, namely, Example 1-9 groups, Comparative Example 1-4 groups and a model group. The remaining 8 mice without modeling were used as a control group. At the beginning of the second week of standing training, they were gavaged with sleep-relieving milk. Each experimental group was gavaged with the sleep-relieving milk product prepared for the corresponding group once a day at a dose of 1 mL / 100 g (mouse body weight). The model group and the control group mice were gavaged with an equal amount of pure water at the same dose. The experiment was carried out for 4 weeks, during which the mice in each group were free to eat and drink.

[0154] (2) The sleep test was conducted in a quiet environment one day before the end of the experiment. The details are as follows:

[0155] ① Pentobarbital sodium induced sleep duration test: In the sleep test, the sleep and wakefulness states were determined by whether the righting reflex disappeared, and the disappearance of the righting reflex for more than 30 seconds was considered to indicate sleep. After 30 minutes of gavage, each group of mice was intraperitoneally administered with pentobarbital sodium (49 mg / kg.bw). The sleep duration was recorded as the time interval from the disappearance of the righting reflex to the recovery. The test results are shown in Table 1.

[0156] ② Pentobarbital sodium induced sleep latency test: drug intervention for 30 minutes, each mouse was intraperitoneally injected with pentobarbital sodium, the dose was 320 mg / kg.bw. The sleep latency was recorded as the time interval from pentobarbital sodium injection to the disappearance of righting reflex, and the test results are shown in Table 1.

[0157] Table 1

[0158] Group Sleep duration (min) Sleep latency (min) Example 1 47.3 25.1 Example 2 45.3 26.2 Example 3 46.1 25.5 Example 4 43.3 27.5 Example 5 42.1 27.1 Example 6 43.6 28.4 Example 7 44.0 28.0 Example 8 42.5 29.4 Example 9 42.8 27.4 Comparative Example 1 40.1 32.1 Comparative Example 2 39.5 30.5 Comparative Example 3 40.5 31.7 Comparative Example 4 41.2 31.8 Model Group 22.5 39.4 Control group 38.6 26.1

[0159] From the data in the table, we can see that:

[0160] (1) Compared with the control group, the sleep duration of mice in the model group was significantly shortened, and the sleep latency was significantly prolonged, indicating that the mice in the model group had sleep disorders and had more difficulty falling asleep.

[0161] (2) It can be seen from Examples 1-3 that the oat kernel extract, lotus seed extract, red date extract and banana extract used in the present invention can be used in combination with theanine, casein hydrolysate and α-lactalbumin components to cooperate with each other to synergistically promote the product's calming and soothing effect and improve sleep.

[0162] (3) By comparing Example 1 with Examples 4-7, it can be seen that the oat kernel extract in the present invention is enzymatically extracted by a composite enzyme composed of four enzymes: α-amylase, cellulase, alkaline protease and flavor protease. The four enzymes can synergistically promote the enzymatic hydrolysis effect after being used in combination, further improving the extraction effect of the relevant active ingredients in the oat kernel, and obtaining an oat kernel extract with better effect.

[0163] (3) By comparing Example 1 with Example 8, it can be seen that the combined treatment of microwave and ultrasound in the present invention can further enhance the enzymatic hydrolysis effect and produce an oat kernel extract with higher activity.

[0164] (4) By comparing Example 1 with Example 9, it can be seen that the lotus seed extract prepared by the method of ultrasound-assisted alcohol extraction in the present invention has a better effect than the corresponding extract prepared by traditional heating reflux extraction.

[0165] (5) By comparing Example 1 with Comparative Examples 1-4, it can be seen that when Comparative Examples 1-4 do not contain any of the four components of oat kernel extract, lotus seed extract, red date extract and banana extract, the effects of the sleeping milk prepared therefrom are worse than those of Example 1. It can be seen that the four components of oat kernel extract, lotus seed extract, red date extract and banana extract used in combination in the present invention can cooperate with each other, synergistically enhance the effect and improve sleep.

[0166] Test Example 2

[0167] Clinical sleep experiment

[0168] 90 adult women aged 20-40 years old (with no history of allergy to any related ingredients and an average deep sleep time of 5-6 hours per day) were selected and divided into 14 groups, with 6 people in each group. Among them, 13 groups drank 250 mL of the sleeping milk product of Examples 1-9 and Comparative Examples 1-4 1 hour before going to bed every day, and the remaining group was a blank group (drinking an equal amount of purified water). After three weeks, the sleep improvement was statistically analyzed. During this period, no sleeping pills or health products were taken, and the same commercially available bracelet was worn to measure the changes in sleep time. The device can measure deep sleep time, which is indirectly used to evaluate the sleep improvement. The results are shown in Table 2.

[0169] Table 2

[0170]

[0171]

[0172] It can be seen from the data in the table that the sleeping milk provided by the present invention can soothe the nerves and promote sleep, and significantly improve the sleeping condition. After the four extracts of oat kernel extract, lotus seed extract, red date extract and banana extract are added, they are combined with theanine, casein hydrolysate and α-lactalbumin components to cooperate with each other and synergistically promote the product's soothing and sleeping effect. Long-term use can further improve sleep and improve sleep quality.

[0173] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0174] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0175] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A sleeping milk, characterized in that: The raw materials of the sleeping milk include raw cow milk, theanine, casein enzymatic hydrolysate, α-lactalbumin, sucrose fatty acid ester, mono- and distearic glycerol, buffer salt and composite extract; The composite extract comprises a combination of oat kernel extract, lotus seed extract, red date extract and banana extract.

2. The sleeping milk according to claim 1, characterized in that: The mass ratio of the oat kernel extract, lotus seed extract, red date extract and banana extract is (1-5):(0.5-2):(2-6):(1-3).

3. The sleeping milk according to claim 1 or 2, characterized in that: The compound extract is added to the sleeping milk in an amount of 0.1 wt%-1 wt%.

4. The sleeping milk according to claim 1 or 2, characterized in that: The oat kernel extract is prepared by a preparation method comprising the following steps: The oat kernels are dried and ground, mixed with a complex enzyme solution, subjected to microwave and ultrasonic co-extraction treatment, the treated solution is centrifuged, the precipitate is mixed with water, and the microwave and ultrasonic co-extraction process is repeated, centrifuged, the supernatant is collected and combined, and freeze-dried to obtain the oat kernel extract; The enzymes in the complex enzyme solution include α-amylase, cellulase, alkaline protease and flavor protease.

5. The sleeping milk according to claim 4, characterized in that: The mass ratio of the α-amylase, cellulase, alkaline protease and flavor protease is (1-3):(2-6):(0.1-4):(0.05-2).

6. The sleeping milk according to claim 4, characterized in that: The amount of the compound enzyme added to the compound enzyme solution is 0.1‰-0.5‰ of the mass of the oat kernel; Preferably, the ratio of the oat kernel to the complex enzyme solution is 1 g: (40-80) mL.

7. The sleeping milk according to claim 1 or 2, characterized in that: The lotus seed extract is prepared by a preparation method comprising the following steps: The lotus seed raw material is crushed and sieved, and then mixed with anhydrous ethanol for extraction under ultrasonic conditions, and the filtrate is filtered and collected, and the filter residue is mixed with anhydrous ethanol to repeat the above extraction and filtration treatment twice, and the filtrate is combined, heated to remove alcohol, and dried to obtain the lotus seed extract.

8. The sleeping milk according to claim 1 or 2, characterized in that: The red date extract and banana extract are prepared by a preparation method comprising the following steps: The red dates or banana raw materials are freeze-dried, crushed and sieved, extracted with hot water, and the filtrate is collected by filtration. Optionally, the filter residue is subjected to the hot water extraction and filtration treatments for 1-2 times, and the filtrates are combined and dried to obtain the product.

9. The sleeping milk according to claim 1, characterized in that: The raw materials of the sleeping milk include 95-99.9 parts of raw milk, 0.01-0.5 parts of theanine, 0.01-0.3 parts of casein hydrolysate, 0.05-0.5 parts of α-lactalbumin, 0.01-0.1 parts of sucrose fatty acid ester, 0.01-0.1 parts of mono- and distearic acid glyceryl, 0.01-0.05 parts of buffer salt and 0.1-1 parts of compound extract in parts by mass; Preferably, the casein enzymatic hydrolysate comprises biologically active decapeptides of the αS1-casein 91-100 fragment; Preferably, the buffer salt is selected from any one of sodium hexametaphosphate, disodium hydrogen phosphate or sodium bicarbonate, or a combination of at least two thereof.

10. A sleeping milk according to any one of claims 1 to 9, characterized in that: The preparation method of the sleeping milk comprises the following steps: Theanine, buffer salt and raw milk are mixed evenly, and then mixed with casein hydrolysate, α-lactalbumin, sucrose fatty acid ester, mono- and distearic acid glycerol and composite extracts, and then homogenized. The homogenized mixture is sterilized to obtain the sleeping milk.

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