An anti-wrinkle composition and its uses, cosmetics

By combining various plant extracts with Spirulina macrophylla extract and Fagus bud solution, a synergistic anti-wrinkle composition is formed, which solves the problem of insufficient anti-wrinkle effect in the prior art, and achieves significant free radical scavenging and elastase inhibition, thus delaying skin aging.

CN119632907BActive Publication Date: 2026-03-13GUANGZHOU MEIZI BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-12-31
Publication Date
2026-03-13

AI Technical Summary

Technical Problem

Existing anti-wrinkle compositions are insufficient in their anti-wrinkle effects and are difficult to effectively prevent skin aging and eliminate free radicals.

Method used

By combining various plant extracts with Spirulina macrophylla extract and Fagus bud solution, and integrating them with resveratrol solution, a synergistic anti-wrinkle composition is formed, enhancing the anti-wrinkle effect of the composition.

Benefits of technology

It significantly improves the free radical scavenging rate and elastase inhibition rate of the anti-wrinkle composition, delays skin aging, and improves skin elasticity and firmness.

✦ Generated by Eureka AI based on patent content.

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Abstract

This invention belongs to the field of cosmetics and discloses an anti-wrinkle composition and its use. The anti-wrinkle composition comprises functional factors, Spirulina macrophylla extract, and Fagus sylvatica bud solution, and the mass ratio of functional factors, Spirulina macrophylla extract, and Fagus sylvatica bud solution is 5-7:0.5-1.5:0.01-0.1. By combining functional factors containing multiple plant extracts with Spirulina macrophylla extract and Fagus sylvatica bud solution, the anti-wrinkle effect of the composition is synergistically enhanced. In addition, a cosmetic product containing 1-30 wt% of the anti-wrinkle composition described above is also disclosed.
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Description

Technical Field

[0001] This invention relates to the field of cosmetics, and more particularly to an anti-wrinkle composition and its uses, and cosmetics. Background Technology

[0002] Skin aging refers to the decline in the skin's external morphology, internal structure, and function caused by exogenous or endogenous factors. Many factors contribute to skin aging, with oxidation being a significant one. Oxidation is the cost of obtaining oxygen through respiration, and it generates free radicals that contribute to aging. Free radicals are a major factor leading to skin damage and aging. Excessive accumulation of free radicals damages the skin primarily through loss of suppleness and elasticity, resulting in wrinkles, dryness, keratinization, dullness, and excessive deposition of reduced / oxidized glutathione and lipofuscin—a series of signs of aging.

[0003] Chinese patent application 202311538944.X discloses a moisturizing, anti-wrinkle, soothing, and firming composition and a cream containing the composition, wherein the composition comprises saffron extract, carnation flower extract, spirulina extract, a moisturizer, and a solvent.

[0004] This formula uses saffron extract, carnation flower extract, spirulina extract, and moisturizers in a synergistic combination to enhance the skin's hydration and moisture absorption, resulting in better deep hydration.

[0005] However, this application found through research that when Spirulina macrophylla extract is combined with European beech bud solution and functional factors containing thyme extract, Myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, and Ganoderma lucidum extract, it also has a good anti-wrinkle effect on the skin.

[0006] The problem this solution aims to solve is: how to propose an anti-wrinkle composition that differs from existing technologies. Summary of the Invention

[0007] To address the aforementioned problems, this invention provides an anti-wrinkle composition and its uses, as well as a cosmetic. The composition, which combines functional factors containing various plant extracts with Spirulina macrophylla extract and Fagus bud solution, forms a composition with excellent anti-wrinkle effects. Furthermore, combining the composition with resveratrol solution can further enhance the anti-wrinkle effect and prevent skin aging.

[0008] To achieve the above objectives, this application discloses an anti-wrinkle composition containing functional factors, Spirulina macrophylla extract, and Fagus spp. bud solution, wherein the mass ratio of functional factors, Spirulina macrophylla extract, and Fagus spp. bud solution is 5–7:0.5–1.5:0.01–0.1.

[0009] The functional factor was prepared by mixing water, butylene glycol, thyme extract, myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, Ganoderma lucidum extract, 1,2-hexanediol, and ethylhexylglycerin in a mass ratio of 55-60:28-32:1:1-3:1-3:1-3:1-3:0.8-1.2:0.1.

[0010] The functional ingredients contain five plant extracts: thyme extract, myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, and black ganoderma extract. Among them, thyme extract has skin-revitalizing and anti-wrinkle properties, which can promote the production of fibrinogen and inhibit the activity of elastase and collagenase, thereby helping to maintain the elasticity and firmness of the skin.

[0011] Myrothamnus flabellifolia leaf extract is rich in natural and effective antioxidants such as polyphenols and flavonoids, which can prevent free radicals from damaging the skin, slow down the skin aging process, and balance skin pigmentation and age spots.

[0012] Roselle flower extract is rich in anthocyanins, vitamin C and other antioxidants, which can eliminate free radicals in the body, delay cell aging and keep the skin elastic and radiant.

[0013] Honeysuckle flower extract contains biflavonoids, which have excellent antioxidant and anti-aging effects, helping to eliminate free radicals and delay skin aging;

[0014] The polysaccharides and polypeptides in black Ganoderma lucidum extract have significant anti-aging effects, can promote and adjust immune function, regulate metabolic balance, promote the synthesis of nucleic acids and proteins, and resist and eliminate free radicals in the body.

[0015] Spirulina maxima is rich in bioactive substances such as beta-carotene, vitamin E, gamma-linolenic acid, and superoxide dismutase. These ingredients can eliminate free radicals and effectively delay the aging process of cells. At the same time, they can also improve skin vitality, improve dullness and yellowing caused by fatigue, and make the skin look healthier and more radiant.

[0016] European beech bud solution can accelerate oxygen entry into the skin, stimulate skin cell energy, and restore cell vitality. This process also helps increase protein synthesis, making the skin youthful and smoothing wrinkles.

[0017] By combining functional factors, Spirulina macrophylla extract, and Fagus bud solution, the anti-wrinkle effect of the composition can be synergistically enhanced.

[0018] Preferably, the composition further contains a resveratrol solution, and the mass ratio of the resveratrol solution to the European beech bud solution is 0.001-0.1:0.01-0.1.

[0019] In addition, the use of the anti-wrinkle composition described above in the preparation of cosmetics is also disclosed.

[0020] In addition, a cosmetic product is disclosed, which contains 1 to 30 wt% of the anti-wrinkle composition as described above.

[0021] Preferably, the cosmetic further contains skin conditioning agents, moisturizers, emulsifiers, antioxidants, and thickeners, and the mass ratio of the skin conditioning agents, moisturizers, emulsifiers, antioxidants, and thickeners is 1-1.5:9-11:0.4-0.6:0.4-0.6:0.05-0.15.

[0022] Preferably, the skin conditioning agent is selected from at least one of niacinamide, arginine / lysine polypeptide solution, and ergothioneine;

[0023] The moisturizer is selected from at least one of butylene glycol, methyl gluceth-20, glycerin, dipropylene glycol, trehalose, 1,2-hexanediol, and sodium hyaluronate.

[0024] The emulsifier is selected from at least one of polysorbate-20 and PEG-40 hydrogenated castor oil;

[0025] The antioxidant is p-hydroxyacetophenone;

[0026] The thickener is selected from at least one of carbomer, acrylate / C10-30 alkanol acrylate crosspolymer, and xanthan gum.

[0027] Preferably, the cosmetic is an essence water or essence liquid.

[0028] The beneficial effects of this invention are:

[0029] This invention provides a composition with anti-wrinkle effects, which combines functional factors containing various plant extracts with Spirulina macrophylla extract and Fagus bud solution to synergistically enhance the anti-wrinkle effect of the composition. Detailed Implementation

[0030] In the description of this invention, it should be noted that unless specific conditions are specified in the examples, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0031] Product Information:

[0032] Functional factor: Provided by Jiangxi Nanwushan Traditional Chinese Medicine Technology Co., Ltd. (trade name: Muscle Age Rhythm Factor F5), it is prepared by mixing water, butylene glycol, thyme extract, myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, black ganoderma extract, 1,2-hexanediol, and ethylhexylglycerin in a mass ratio of 59.9:30:1:2:2:2:2:1:0.1.

[0033] Spirulina macrophylla extract: provided by Guangdong Gexin Biotechnology Co., Ltd.

[0034] European beech bud solution: provided by Jiafashi (Shanghai) Trading Co., Ltd., it is prepared by mixing water, European beech bud extract and phenoxyethanol in a mass ratio of 83:15:2;

[0035] Resveratrol solution: provided by Morechem Co., Ltd., wherein the mass fraction of resveratrol in the solution is 4%;

[0036] Arginine / lysine peptide solution: supplied by Activen SA, trade name XEP-018, wherein the mass fraction of arginine / lysine peptide in the solution is 0.0024%.

[0037] Unless otherwise specified, the preparation method of the anti-wrinkle composition in the following examples and comparative examples is as follows: the functional factor, Spirulina macrophylla extract, Fagus sylvatica bud solution, and resveratrol solution are mixed at 85°C for 5 min to obtain the anti-wrinkle composition.

[0038] Examples 1-4

[0039] An anti-wrinkle composition, wherein the mass ratios of the raw materials in its formulation are shown in Table 1:

[0040] Table 1

[0041]

[0042] Comparative Examples 1-11

[0043] An anti-wrinkle composition, the mass ratios of each raw material in its formulation are shown in Table 2:

[0044] Table 2

[0045] Group Functional factors Spirulina macrophylla extract European beech bud solution Other ingredients Comparative Example 1 0 1.0 0.05 0 Comparative Example 2 6 0 0.05 0 Comparative Example 3 6 1.0 0 0 Comparative Example 4 0 1.0 0.05 6 Comparative Example 5 0 1.0 0.05 6 Comparative Example 6 0 1.0 0.05 6 Comparative Example 7 0 1.0 0.05 6 Comparative Example 8 0 1.0 0.05 6 Comparative Example 9 6 0 0.05 1.0 Comparative Example 10 6 1.0 0 0.05 Comparative Example 11 6 1.0 0 0.05

[0046] It should be noted that:

[0047] The other components mentioned in Comparative Examples 4-8 are functional factors prepared by mixing water, butylene glycol, plant extracts, 1,2-hexanediol, and ethylhexylglycerin in a mass ratio of 59.9:30:9:1:0.1. The plant extracts in the functional factors of Comparative Examples 4-8 are thyme extract, myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, and black ganoderma extract, respectively.

[0048] The other ingredients in Comparative Example 9 were commercially available Spirulina platensis extract;

[0049] The other ingredients in Comparative Example 10 were commercially available Astragalus extract;

[0050] The other component in Comparative Example 11 was a resveratrol solution.

[0051] Application Example 1

[0052] The formula of a cosmetic product, by mass fraction, is shown in Table 3:

[0053] Table 3

[0054] Group Anti-wrinkle composition Skin conditioning agent Moisturizer emulsifier antioxidants Thickener water Application Example 1 7% 1.3% 10% 0.5% 0.5% 0.1% To 100%

[0055] It should be noted that:

[0056] The cosmetic is prepared by mixing the anti-wrinkle composition obtained in Example 1, skin conditioning agent, moisturizer, emulsifier, antioxidant and thickener, heating to 85°C and homogenizing for 5 minutes, and then cooling to 30°C to obtain the cosmetic.

[0057] In Application Example 1, the skin conditioning agent is an arginine / lysine polypeptide solution;

[0058] In Application Example 1, the moisturizer is composed of butylene glycol, glycerin, and trehalose, and the mass ratio of butylene glycol, glycerin, and trehalose is 4:1:0.5.

[0059] In Application Example 1, the emulsifier is polysorbate-20;

[0060] In Application Example 1, the antioxidant is p-hydroxyacetophenone;

[0061] In Application Example 1, the thickener is carbomer.

[0062] Application Example 2

[0063] It is basically the same as Application Example 1, except that the cosmetic does not contain skin conditioning agents.

[0064] Application Example 3

[0065] It is basically the same as Application Example 1, except that the cosmetic does not contain moisturizer.

[0066] Application Example 4

[0067] It is basically the same as Application Example 1, except that the cosmetic does not contain antioxidants.

[0068] Performance testing:

[0069] 1. DPPH free radical scavenging rate test

[0070] Excessive production of DPPH free radicals can lead to natural skin aging and photoaging, resulting in wrinkles. Therefore, the ability to scavenge free radicals is one of the important indicators for evaluating anti-aging and antioxidant cosmetics (raw materials).

[0071] This experiment references the laboratory method (HMC-WI-030DPPH free radical scavenging rate). The results of the DPPH free radical scavenging rate test of the test sample and the negative control are compared. If the scavenging rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), the test sample can be considered to have a certain antioxidant effect.

[0072] 1.1 Instruments and Equipment

[0073] BSA224S analytical balance, L6s ultraviolet spectrophotometer

[0074] 1.2 Reagents

[0075] DPPH (1,1-diphenyl-2-picrylhydrazine), 98%

[0076] 1.3 Test Methods

[0077] (1) Processing of control materials and test samples

[0078] Sample group: solutions of the compositions prepared in Examples 1-4 and Comparative Examples 1-11 diluted with pure water to a mass fraction of 7%; cosmetics prepared in Examples 1-4;

[0079] Negative control: pure water.

[0080] (2) Experimental Operation Procedures

[0081] Set up a sample group and a negative control group, with three parallel tubes for each group. Add the corresponding reagent solution to each group, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solution of each group into a 1 cm cuvette and measure the absorbance at 517 nm.

[0082] (3) The calculation formula is shown in Equation 1:

[0083]

[0084] Where: T—absorbance of the sample tube, i.e., absorbance of the solution after the sample reacts with DPPH;

[0085] T0—Sample background absorbance;

[0086] The average of three absorbance values ​​of the C-DPPH tube is the absorbance value of the DPPH solution without the addition of sample.

[0087] C0 — Solvent background absorbance.

[0088] (4) Data Analysis

[0089] Statistical analysis was performed using SPSS software. Independent samples t-tests were used to compare the DPPH free radical scavenging rates between the test samples and the negative control. All statistical analyses were two-tailed tests, with a significance level of α = 0.05. P > 0.05 indicated no significant difference between the two groups; P < 0.05 indicated a significant difference between the two groups.

[0090] The test results are shown in Table 4:

[0091] Table 4

[0092]

[0093] Conclusion Analysis:

[0094] 1. As can be seen from Examples 1-3, when the mass ratio of the functional factors, Spirulina macrophylla extract, and Fagus bud solution in the anti-wrinkle composition is changed, the scavenging rate of free radicals also varies, and the ratio in Application Example 1 is the best.

[0095] Further observation of Examples 1 and 4 shows that when resveratrol solution is added to the anti-wrinkle composition, the scavenging rate of free radicals can be further improved because resveratrol can directly react with free radicals, reduce the activity of free radicals, and prevent further chain reactions of free radicals.

[0096] Further observation of Examples 1, 4, and Comparative Example 11 reveals that when the amount of functional factor and Spirulina macrophylla extract added to the anti-wrinkle composition remains unchanged, the free radical scavenging rate of the European beech bud solution and resveratrol solution mixed in equal proportions should be 41.288% ((free radical scavenging rate of Example 1 + free radical scavenging rate of Comparative Example 11) / 2), while that of Example 4 reached 42.863%. This indicates that there is a synergistic effect between resveratrol solution and functional factor, Spirulina macrophylla extract, and European beech bud solution.

[0097] 2. As can be seen from Example 1 and Comparative Examples 1-3, when the anti-wrinkle composition lacks functional factors, Spirulina macrophylla extract or Fagus bud solution, the free radical scavenging rate will decrease significantly. Among them, the cosmetics made without functional factors have the largest decrease, reaching 22.731%.

[0098] Further analysis of the data in Table 4 shows that the anti-wrinkle composition formed by the functional factor, Spirulina macrophylla extract, and Fagus bud solution has a free radical scavenging rate of 35.979% ((Free radical scavenging rate of Comparative Example 1 + Free radical scavenging rate of Comparative Example 2 + Free radical scavenging rate of Comparative Example 3) / 2). The anti-wrinkle composition prepared in Example 1 has a free radical scavenging rate of 40.922%. This indicates that the functional factor, Spirulina macrophylla extract, and Fagus bud solution have a synergistic effect, further improving the free radical scavenging rate of the anti-wrinkle composition.

[0099] 3. As can be seen from Example 1 and Comparative Examples 4-8, when the functional factors in the anti-wrinkle composition contain only one plant extract, its free radical scavenging rate will also decrease significantly compared with Example 1.

[0100] Furthermore, observation of Comparative Examples 4-8 revealed that when the functional factor contains five plant extracts—thyme extract, myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, and black Ganoderma lucidum extract—the free radical scavenging rate of the resulting anti-wrinkle composition should be between 28.933% and 30.871%, while Example 1 achieved 40.922%. This indicates that the five plant extracts in the functional factor—thyme extract, myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, and black Ganoderma lucidum extract—can produce a synergistic effect, further enhancing the scavenging rate of DPPH free radicals.

[0101] 4. As can be seen from Example 1 and Comparative Example 9, when commercially available Spirulina platensis extract is used to replace Spirulina macrophylla extract in the anti-wrinkle composition, the scavenging rates of the two for DPPH free radicals are not the same. It is speculated that the two may be different in terms of the types and contents of their components, which may result in a worse effect when combined with functional factors and European beech bud solution than in Application Example 1.

[0102] Similarly, observations of Example 1 and Comparative Example 10 show that when the European beech bud solution in the anti-wrinkle composition was replaced with commercially available astragalus extract, which also helps protein synthesis and smooths wrinkles, Comparative Example 10 also achieved poor results.

[0103] Therefore, the Spirulina macrophylla extract and Fagus bud solution in the anti-wrinkle composition are irreplaceable.

[0104] 5. As can be seen from Example 1 and Application Example 1, when a skin conditioning agent, moisturizer, and antioxidant are simultaneously combined with an anti-wrinkle composition to prepare a cosmetic, Application Example 1 also shows a significant improvement in the free radical scavenging rate compared to Example 1.

[0105] Further observation of application examples 1-4 shows that when skin conditioning agents, moisturizers, and antioxidants are lacking in cosmetics, it will also affect the cosmetics' free radical scavenging rate for DPPH.

[0106] 2. Elastase inhibition rate test

[0107] Elastase is mainly synthesized and secreted by fibroblasts and can degrade elastin in the skin, leading to skin aging. The experimental principle of inhibiting elastase is that porcine pancreatic elastase undergoes a catalytic reaction with its substrate. After adding the active substance, the absorbance changes, and the magnitude of the change in absorbance reflects the inhibition rate of the elastase inhibitor.

[0108] This experiment references the laboratory method (HMC-WI-028 elastase inhibition rate). The results of the elastase inhibition rate test of the test sample and the negative control were compared. If the inhibition rate of the test sample was higher than that of the negative control and the difference was statistically significant (P<0.05), the test sample could be considered to have a certain firming effect.

[0109] 2.1 Instruments and Equipment

[0110] BSA224S analytical balance, RT-6100 enzyme-linked immunosorbent assay (ELISA) analyzer;

[0111] 2.2 Reagents

[0112] Elastase (porcine pancreas), BR, N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%;

[0113] 2.3 Test Methods

[0114] (1) Processing of control materials and test samples

[0115] Sample group: The compositions prepared in Examples 1-4 and Comparative Examples 1-11 were diluted with pure water to a solution with a composition mass fraction of 7%; and the cosmetics prepared in Examples 1-4 were used.

[0116] Negative control: pure water.

[0117] (2) Experimental Operation Procedures

[0118] Set up a sample group and a negative control group, with 3 replicates for each group. Add the corresponding reagent solution to each well of a 96-well plate, shake gently, incubate at 25°C for 15 min, and then place it in an ELISA reader to measure the absorbance at 410 nm.

[0119] (3) The calculation formula is shown in Equation 2:

[0120]

[0121] In the formula: A — is the absorbance of the reaction solution without the sample;

[0122] B—The absorbance of the reaction solution without the sample and enzyme;

[0123] C—is the absorbance of the reaction solution containing the sample and enzyme;

[0124] D—The absorbance of the reaction solution containing the sample and the reaction solution without the enzyme.

[0125] (4) Data Analysis

[0126] Statistical analysis was performed using SPSS software. Independent samples t-tests were used to compare the elastase inhibition rates of the experimental samples and the negative control. All statistical analyses were two-tailed tests, with a significance level of α = 0.05. P > 0.05 indicated no significant difference between the two groups; P < 0.05 indicated a significant difference between the two groups.

[0127] The test results are shown in Table 5:

[0128] Table 5

[0129]

[0130]

[0131] Conclusion Analysis:

[0132] 1. As can be seen from Examples 1-3, when the mass ratio of the functional factors, Spirulina macrophylla extract, and Fagus bud solution in the anti-wrinkle composition is changed, the inhibition rate of elastin also varies, and the ratio in Example 1 is the best.

[0133] Further observation of Examples 1 and 4 shows that when resveratrol solution is added to the anti-wrinkle composition, the inhibition rate of elastin can be further improved because resveratrol can directly react with free radicals, reduce the activity of free radicals, and prevent further chain reactions of free radicals.

[0134] Further observation of Examples 1, 4, and Comparative Example 11 reveals that when the amount of functional factor and Spirulina macrophylla extract added to the anti-wrinkle composition remains unchanged, the inhibition rate of elastase by the mixture of European beech bud solution and resveratrol solution in equal proportions should be 42.596% ((free radical scavenging rate of Example 1 + free radical scavenging rate of Comparative Example 11) / 2), while that of Example 4 reaches 44.782%. This indicates that there is a synergistic effect between resveratrol solution and functional factor, Spirulina macrophylla extract, and European beech bud solution.

[0135] 2. As can be seen from Example 1 and Comparative Examples 1-3, when the anti-wrinkle composition lacks functional factors, Spirulina macrophylla extract or Fagus bud solution, the inhibition rate of the anti-wrinkle composition against elastase will decrease significantly. Among them, the cosmetics made without functional factors showed the largest decrease, reaching 23.038%.

[0136] Further analysis of the data in Table 5 shows that the anti-wrinkle composition formed by the functional factor, Spirulina macrophylla extract, and Fagus bud solution has an elastase inhibition rate of 35.344% ((elastase inhibition rate of Comparative Example 1 + elastase inhibition rate of Comparative Example 2 + elastase inhibition rate of Comparative Example 3) / 2). The anti-wrinkle composition prepared in Example 1 has a free radical scavenging rate of 42.365%. Therefore, it can be seen that the functional factor, Spirulina macrophylla extract, and Fagus bud solution have a synergistic effect, further improving the elastase inhibition rate of the anti-wrinkle composition.

[0137] 3. As can be seen from Example 1 and Comparative Examples 4-8, when the functional factor in the anti-wrinkle composition contains only one plant extract, its inhibition rate against elastase will also decrease significantly compared with Example 1.

[0138] Furthermore, observation of Comparative Examples 4-8 revealed that when the functional factors contain five plant extracts—thyme extract, myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, and black ganoderma extract—the elastase inhibition rate of the resulting anti-wrinkle composition should be between 27.689% and 30.565%, while Example 1 achieved 42.365%. This indicates that the five plant extracts in the functional factors—thyme extract, myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, and black ganoderma extract—can produce a synergistic effect, further enhancing the elastase inhibition rate of the cosmetic.

[0139] 4. As can be seen from Example 1 and Comparative Example 9, when commercially available Spirulina platensis extract is used to replace Spirulina macrophylla extract in the anti-wrinkle composition, the inhibition rate of elastase in the cosmetics prepared by the two is not the same. It is speculated that the two may be different in terms of the types and contents of the ingredients, so the effect of the combination with functional factors and European beech bud solution is worse than that of Application Example 1.

[0140] Similarly, observations of Example 1 and Comparative Example 10 show that when the European beech bud solution in the anti-wrinkle composition was replaced with a commercially available astragalus extract that can also help protein synthesis and smooth wrinkles, the effect obtained in Application Example 14 was also poor.

[0141] Therefore, the Spirulina macrophylla extract and Fagus bud solution in the anti-wrinkle composition are irreplaceable.

[0142] 5. As can be seen from Example 1 and Application Example 1, when a skin conditioning agent, moisturizer, and antioxidant are simultaneously combined with an anti-wrinkle composition to prepare a cosmetic, Application Example 1 also shows a significant improvement in the inhibition rate of elastase compared to Example 1.

[0143] Further observation of application examples 1-4 shows that when skin conditioning agents, moisturizers, and antioxidants are lacking in cosmetics, they also affect the inhibition rate of elastase by the cosmetics.

[0144] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. An anti-wrinkle composition, characterized in that, The composition comprises functional factors, Spirulina macrophylla extract, Fagus spp. bud solution, and resveratrol solution, wherein the mass ratio of functional factors, Spirulina macrophylla extract, Fagus spp. bud solution, and resveratrol solution is 5-7:0.5-1.5:0.01-0.1:resveratrol solution; The functional factor was prepared by mixing water, butylene glycol, thyme extract, myrothamnus flabellifolia leaf extract, hibiscus flower extract, honeysuckle flower extract, Ganoderma lucidum extract, 1,2-hexanediol, and ethylhexylglycerin in a mass ratio of 55-60:28-32:1:1-3:1-3:1-3:1-3:0.8-1.2:0.

1.

2. Use of the anti-wrinkle composition according to any one of claims 1 in the preparation of cosmetics.

3. A cosmetic product, characterized in that, The cosmetic contains 1 to 30 wt% of the anti-wrinkle composition as described in any one of claims 1-2.

4. The cosmetic product according to claim 3, characterized in that, The cosmetic also contains skin conditioning agents, moisturizers, emulsifiers, antioxidants, and thickeners, and the mass ratio of skin conditioning agents, moisturizers, emulsifiers, antioxidants, and thickeners is 1-1.5: 9-11: 0.4-0.6: 0.4-0.6: 0.05-0.

15.

5. The cosmetic product according to claim 4, characterized in that, The skin conditioning agent is selected from at least one of niacinamide, arginine / lysine polypeptide solution, and ergothioneine; The moisturizer is selected from at least one of butylene glycol, methyl gluceth-20, glycerin, dipropylene glycol, trehalose, 1,2-hexanediol, and sodium hyaluronate. The emulsifier is selected from at least one of polysorbate-20 and PEG-40 hydrogenated castor oil; The antioxidant is p-hydroxyacetophenone; The thickener is selected from at least one of carbomer, acrylate / C10-30 alkanol acrylate crosspolymers, and xanthan gum.

6. The cosmetic product according to claim 3, characterized in that, The cosmetic product in question is an essence water.

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