Gynostemma pentaphylla composition as well as preparation method and application thereof

Abstract

The invention mainly relates to a gynostemma pentaphyllum composition as well as a preparation method and application thereof, and belongs to the technical field of biology. The composition comprises 8-12 parts of fiveleaf gynostemma herb, 8-12 parts of barbary wolfberry fruit, 6-10 parts of honeysuckle flower and 3-7 parts of orange. The gynostemma pentaphyllum composition is prepared by the following steps: performing alcohol extraction on gynostemma pentaphyllum, fructus lycii and exocarpium citri rubrum to obtain a solid part, performing water extraction on the solid part to obtain a water extract 1, performing water extraction on honeysuckle to obtain a water extract 2, and mixing the water extract 1 and the water extract 2 to obtain the gynostemma pentaphyllum composition. All the raw materials are compatible with one another and can play a synergistic role, meanwhile, functional components can be better extracted through the extraction method, and then the efficacy of the composition is improved. The composition prepared by the invention can increase the exercise tolerance of the body, improve the metabolic ability to serum urea and lactic acid to adapt to the load state of the body, increase the energy reserve of the body and achieve the effect of effectively relieving physical fatigue.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a gynostemma pentaphyllum composition and a preparation method and application thereof. Background Art

[0002] Fatigue refers to a state in which the body's labor efficiency tends to decline due to excessively long or overly intense physical or mental labor under certain environmental conditions. With the development of society and the acceleration of the pace of life, people's work pressure continues to increase, and their dietary structure and living habits have also changed. Long-term overload labor, lack of exercise and irregular diet make people's bodies in a state of fatigue for a long time. If they are in a state of fatigue for a long time, physiological functions will be reduced or destroyed, various diseases will be caused, leading to a decline in immune function and endocrine disorders, and immune system diseases such as colds, cardiovascular diseases, and diabetes will occur. Therefore, relieving physical fatigue is the key to preventing the invasion of various diseases. There are still no clear research results on physical fatigue. It is speculated that the cause may be related to infection, abnormal immune system, endocrine and metabolic disorders, and neuropsychiatric diseases; at the same time, social environment, psychological factors and genetic factors also have an important influence on its development. Therefore, it is of great significance to provide a composition that can effectively relieve physical fatigue. Summary of the invention

[0003] In view of this, the object of the present invention is to provide a Gynostemma pentaphyllum composition that can effectively relieve physical fatigue.

[0004] The invention provides a gynostemma pentaphyllum composition, which comprises the following raw materials in parts by weight: 8-12 parts of gynostemma pentaphyllum, 8-12 parts of wolfberry, 6-10 parts of honeysuckle and 3-7 parts of orange peel.

[0005] Preferably, the Gynostemma pentaphyllum composition comprises the following raw materials in parts by weight: 9-11 parts of Gynostemma pentaphyllum, 9-11 parts of Lycium barbarum, 7-9 parts of Honeysuckle, and 4-6 parts of Citrus aurantium.

[0006] The present invention also provides a method for preparing the Gynostemma pentaphyllum composition, comprising the following steps:

[0007] After extracting Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium with ethanol, the solid part is taken out and water is added to perform water extraction 1 to obtain water extract 1;

[0008] Add water to honeysuckle to perform water extraction 2 to obtain water extract 2;

[0009] The water extract 1 and the water extract 2 are mixed to obtain a Jiaogulan composition.

[0010] Preferably, the mass volume ratio of the Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium to ethanol is 1 g: 8-12 mL.

[0011] Preferably, the ethanol extraction step comprises: extracting at 38-42° C. for 30-38 min.

[0012] Preferably, the mass volume ratio of the Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium to water is 1 g: 18-22 mL.

[0013] Preferably, the water extraction step 1 comprises: extracting at 55-65° C. for 28-32 min.

[0014] Preferably, the mass volume ratio of the honeysuckle to water is 1 g: 18-22 mL.

[0015] Preferably, the water extraction step 2 comprises: extracting at 55-65° C. for 28-32 min.

[0016] The present invention also provides the use of the above-mentioned Gynostemma pentaphyllum composition or the preparation method thereof in the preparation of a product for relieving physical fatigue.

[0017] Beneficial effects of the present invention: The present invention provides a gynostemma pentaphyllum composition, which is composed of gynostemma pentaphyllum, wolfberry, honeysuckle and tangerine peel. The raw materials are compatible with each other to achieve synergistic effects. At the same time, by discarding the alcohol extracts of gynostemma pentaphyllum, wolfberry and tangerine peel and then extracting with water, and extracting with honeysuckle separately, the effective ingredients of the present invention can be better extracted to enhance the efficacy of the composition. The prepared composition can increase the body's sports endurance, improve the metabolic capacity of serum urea and lactic acid to adapt to the body's load state, increase the body's energy reserves, and achieve the effect of effectively relieving physical fatigue. DETAILED DESCRIPTION

[0018] The invention provides a gynostemma pentaphyllum composition, comprising the following raw materials in parts by weight: 8-12 parts of gynostemma pentaphyllum, 8-12 parts of wolfberry, 6-10 parts of honeysuckle, and 3-7 parts of orange peel; preferably 9-11 parts of gynostemma pentaphyllum, 9-11 parts of wolfberry, 7-9 parts of honeysuckle, and 4-6 parts of orange peel; more preferably 10 parts of gynostemma pentaphyllum, 10 parts of wolfberry, 8 parts of honeysuckle, and 5 parts of orange peel.

[0019] In the present invention, Gynostemma pentaphyllum is the dried aerial part of Gynostemma pentaphyllum (Thunb) Mak, a plant of the Cucurbitaceae family; Lycium barbarum is the dried mature fruit of Lycium barbarum L, a plant of the Solanaceae family; Honeysuckle is the dried flower buds or flowers with initial opening of Lonicerajaponica Thunb., a plant of the Caprifoliaceae family; and Citrus reticulata, also called Citrus reticulata, is the dried outer pericarp of Citrus reticulata Blanco, a plant of the Rutaceae family, and its cultivars. The present invention does not specifically limit the sources of the Gynostemma pentaphyllum, Lycium barbarum, Honeysuckle and Citrus reticulata, and conventional commercial products in the art can be used.

[0020] The present invention also provides a method for preparing the Gynostemma pentaphyllum composition, comprising the following steps:

[0021] After extracting Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium with ethanol, the solid portion was taken out and water was added to perform water extraction 1 to obtain water extract 1.

[0022] In the present invention, the volume fraction of the ethanol is preferably 70% to 80%, and in one embodiment, 70%, 72%, 75%, 78% or 80% can be selected. The mass volume ratio of the gynostemma pentaphyllum, wolfberry fruit and orange red to ethanol is preferably 1g:8-12mL, and in one embodiment, 1g:8mL, 1g:9mL, 1g:10mL, 1g:11mL or 1g:12mL can be selected. The ethanol extraction step preferably includes: extraction at 38-42°C for 30-38 min, and in one embodiment, extraction at 38°C, 39°C, 40°C, 41°C or 42°C for 30 min, 31 min, 32 min, 33 min, 34 min, 35 min, 36 min, 37 min or 38 min can be selected; after the ethanol extraction, preferably filtering is performed, and the filtrate is discarded after filtration to obtain a solid residue, which is filtered and dried and then added with water for water extraction 1. The mass volume ratio of the Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium to water is preferably 1g:18-22mL, and in one embodiment, 1g:18mL, 1g:19mL, 1g:20mL, 1g:21mL or 1g:22mL can be selected. The step of water extraction 1 preferably includes: extraction at 55-65°C for 28-32 minutes. In one embodiment, 55°C, 56°C, 58°C, 60°C, 62°C, 64°C or 65°C can be selected for extraction for 28 minutes, 29 minutes, 30 minutes, 31 minutes or 32 minutes. After water extraction 1, it is preferably filtered to obtain the filtrate to obtain water extract 1. The present invention does not specifically limit the filtering method, and conventional filtering methods in the art can be used.

[0023] Then, water is added to the honeysuckle to perform water extraction 2 to obtain water extract 2.

[0024] In the present invention, the mass volume ratio of the honeysuckle to water is preferably 1g: 18-22mL. In one embodiment, 1g: 18mL, 1g: 19mL, 1g: 20mL, 1g: 21mL or 1g: 22mL can be selected. The step of water extraction 2 preferably includes: extracting at 55-65°C for 28-32min. In one embodiment, 55°C, 56°C, 58°C, 60°C, 62°C, 64°C or 65°C can be selected for extraction for 28min, 29min, 30min, 31min or 32min. After the water extraction 2, it is preferably filtered, and the filtrate is taken to obtain the water extract 2. The present invention does not specifically limit the filtering method, and the conventional filtering method in the art can be used.

[0025] The water extract 1 and the water extract 2 are then mixed to obtain a Gynostemma pentaphyllum composition. The mixture may be preferably concentrated. The present invention has no particular limitation on the concentration method, and conventional concentration methods in the art may be used.

[0026] The present invention also provides the use of the above-mentioned Gynostemma pentaphyllum composition or its preparation method in the preparation of a product for relieving physical fatigue. The composition can increase the body's sports endurance, improve the metabolic capacity of serum urea and lactic acid to adapt to the body's load state, increase the body's energy reserves, and achieve the effect of effectively relieving physical fatigue.

[0027] In the present invention, the product is preferably taken orally. The present invention has no special limitation on the dosage form of the product, and any conventional oral dosage form in the art can be selected. In one embodiment, an oral liquid or beverage dosage form can be selected.

[0028] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0029] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0030] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0031] Example 1

[0032] Formula: Gynostemma pentaphyllum 10g, Lycium barbarum 10g, Honeysuckle 8g, Tangerine peel 5g

[0033] 1. Weigh each raw material according to the formula;

[0034] 2. Add 250 mL of 75% ethanol to Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium, heat to 40°C, extract for 35 min, filter, discard the filtrate, add 500 mL of water to the filter residue, heat to 60°C, extract for 30 min, filter, and take the filtrate to obtain water extract 1;

[0035] 3. Add 160 mL of water to honeysuckle and heat to 60°C to extract for 30 min, filter, and take the filtrate to obtain water extract 2;

[0036] 4. The water extract 1 and the water extract 2 are mixed and concentrated to obtain a Gynostemma pentaphyllum composition.

[0037] Example 2

[0038] Formula: Gynostemma pentaphyllum 8g, Lycium barbarum 12g, Honeysuckle 6g, Tangerine peel 7g

[0039] 1. Weigh each raw material according to the formula;

[0040] 2. Take Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium, add 216 mL of 70% ethanol, heat to 38°C, extract for 38 minutes, filter, discard the filtrate, add 486 mL of water to the filter residue, heat to 55°C, extract for 28 minutes, filter, and take the filtrate to obtain water extract 1;

[0041] 3. Add 108 mL of water to honeysuckle and heat to 55°C for 28 min, filter, and take the filtrate to obtain water extract 2;

[0042] 4. The water extract 1 and the water extract 2 are mixed and concentrated to obtain a Gynostemma pentaphyllum composition.

[0043] Example 3

[0044] Formula: Gynostemma pentaphyllum 12g, Lycium barbarum 8g, Honeysuckle 10g, Tangerine peel 3g

[0045] 1. Weigh each raw material according to the formula;

[0046] 2. Take Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium, add 276 mL of 80% ethanol, heat to 42°C, extract for 30 min, filter, discard the filtrate, add 506 mL of water to the filter residue, heat to 65°C, extract for 32 min, filter, and take the filtrate to obtain water extract 1;

[0047] 3. Add 220 mL of water to honeysuckle and heat to 65°C for 32 min, filter and take the filtrate to obtain water extract 2;

[0048] 4. The water extract 1 and the water extract 2 are mixed and concentrated to obtain a Gynostemma pentaphyllum composition.

[0049] Comparative Example 1

[0050] Formula: Gynostemma pentaphyllum 10g, Lycium barbarum 10g, Tangerine peel 5g

[0051] 1. Weigh each raw material according to the formula;

[0052] 2. Take Gynostemma pentaphyllum, wolfberry and orange peel, add 250 mL of 75% ethanol, heat to 40°C, extract for 35 minutes, filter, discard the filtrate, add 500 mL of water to the filter residue, heat to 60°C, extract for 30 minutes, filter, and concentrate the filtrate to obtain a Gynostemma pentaphyllum composition.

[0053] Comparative Example 2

[0054] Formula: Gynostemma pentaphyllum 10g, Lycium barbarum 10g, American ginseng 8g, Tangerine peel 5g

[0055] 1. Weigh each raw material according to the formula;

[0056] 2. Add 250 mL of 75% ethanol to Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium, heat to 40°C, extract for 35 min, filter, discard the filtrate, add 500 mL of water to the filter residue, heat to 60°C, extract for 30 min, filter, and take the filtrate to obtain water extract 1;

[0057] 3. Heat 160 mL of water to 60°C and extract for 30 min, filter, and take the filtrate to obtain water extract 2;

[0058] 4. The water extract 1 and the water extract 2 are mixed and concentrated to obtain a Gynostemma pentaphyllum composition.

[0059] Comparative Example 3

[0060] Formula: Gynostemma pentaphyllum 10g, Lycium barbarum 10g, Honeysuckle 8g, Tangerine peel 5g

[0061] 1. Weigh each raw material according to the formula;

[0062] 2. Take Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium, add 500 mL of water, heat to 60°C, extract for 30 min, filter, and take the filtrate to obtain water extract 1;

[0063] 3. Add 160 mL of water to honeysuckle and heat to 60°C to extract for 30 min, filter, and take the filtrate to obtain water extract 2;

[0064] 4. The water extract 1 and the water extract 2 are mixed and concentrated to obtain a Gynostemma pentaphyllum composition.

[0065] Comparative Example 4

[0066] Formula: Gynostemma pentaphyllum 10g, Lycium barbarum 10g, Honeysuckle 8g, Tangerine peel 5g

[0067] 1. Weigh each raw material according to the formula;

[0068] 2. Take Gynostemma pentaphyllum, wolfberry, honeysuckle and orange peel, add 330 mL of 75% ethanol, heat to 40°C, extract for 35 minutes, filter, discard the filtrate, add 660 mL of water to the filter residue, heat to 60°C, extract for 30 minutes, filter, and concentrate the filtrate to obtain a Gynostemma pentaphyllum composition.

[0069] Comparative Example 5

[0070] Formula: Gynostemma pentaphyllum 10g, Lycium barbarum 10g, Honeysuckle 8g, Tangerine peel 5g

[0071] 1. Weigh each raw material according to the formula;

[0072] 2. Take Gynostemma pentaphyllum, wolfberry and orange peel, add 250 mL of 75% ethanol, heat to 40°C, extract for 35 minutes, filter, discard the filtrate, add honeysuckle to the filter residue after draining the water, then add 660 mL of water, heat to 60°C, extract for 30 minutes, filter, and concentrate the filtrate to obtain a Gynostemma pentaphyllum composition.

[0073] Test Example 1

[0074] 1. Experimental Grouping

[0075] 280 male Kunming mice weighing 18-20g were randomly divided into 4 batches (batch 1, batch 2, batch 3, batch 4), each batch had 7 groups, and each group had 10 mice. That is, blank group, Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group. The blank group was gavaged with distilled water, and the Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group were gavaged with equal doses of the composition prepared by Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, and Comparative Example 5, and the gavage dose was 5.5g / kg in terms of crude drug amount. The gavage volume of each group was 20mL / kg, once a day, for 30 consecutive days. Each index was measured 30 minutes after the last gavage.

[0076] The animals were weighed weekly. All mice were in good health during the experiment. There were no statistically significant differences in the initial body weight and weight gain of mice among the groups.

[0077] 2. Index determination:

[0078] 2.1 Weighted swimming test

[0079] 30 minutes after the last oral gavage of a batch of mice, the mice were placed in a swimming box (50cm×50cm×40cm) for swimming, with a water depth of 35cm and a water temperature of 25℃±1℃. The base of the mouse tail was loaded with lead equivalent to 5% of the mouse's body weight. The time from the start of swimming to the death of the mouse was recorded as the swimming time of the mouse, and the average swimming time of each mouse was taken as the swimming time of the group of mice. The results are shown in Table 1. The weighted swimming time of each group of mice in the embodiment and the comparative example was significantly longer than that of the blank group, indicating that the composition of each group can enhance the exercise endurance of mice, among which the effect of Example 1 is the best, indicating that the composition prepared by the prescription and preparation method of the present invention has the best effect in relieving physical fatigue.

[0080] Table 1 Weighted swimming time of mice in each group

[0081] Grouping Weighted swimming time (s) Blank Group 365.12 Example 1 613.45 Comparative Example 1 499.29 Comparative Example 2 538.23 Comparative Example 3 486.47 Comparative Example 4 514.31 Comparative Example 5 536.33

[0082] 2.2 Serum urea determination

[0083] 30 minutes after the last gavage of the two batches of mice, they swam in water at a temperature of 30°C without weight for 90 minutes, and blood was collected after resting for 60 minutes. The eyeballs of the mice were removed and about 0.5 mL of whole blood (without anticoagulant) was collected. The blood was placed in a 4°C refrigerator for 3 hours, and after the blood coagulated, it was centrifuged at 2000r / min for 15 minutes, and the serum was collected. The urea content in the serum was determined using a kit, and the average value of the urea content in the serum of each mouse was taken as the serum urea content of the group of mice. The results are shown in Table 2. The urea content of mice in each group of the embodiments and comparative examples was lower than that in the blank group, indicating that the composition of each group can improve the ability of mice to remove urea from serum, among which Example 1 has the best effect, indicating that the composition prepared by the prescription and preparation method of the present invention can better improve the adaptability of mice to load and achieve the effect of relieving physical fatigue.

[0084] Table 2 Serum urea content of mice in each group

[0085] Grouping Serum urea content (mmol / L) Blank Group 12.56 Example 1 9.12 Comparative Example 1 10.98 Comparative Example 2 10.32 Comparative Example 3 11.35 Comparative Example 4 10.82 Comparative Example 5 10.07

[0086] 2.3 Glycogen determination

[0087] The three batches of mice were killed 30 minutes after the last oral administration, and the liver was rinsed with physiological saline and dried with filter paper. 100 mg of liver was accurately weighed, 8 mL of TCA was added, and each tube was homogenized for 1 minute. The homogenate was poured into a centrifuge tube and centrifuged at 3000 r / min for 15 minutes. The supernatant was taken and the liver glycogen was measured by anthrone method at a wavelength of 620 nm. The average value of the liver glycogen content of each mouse was taken as the liver glycogen content of the mice in this group. The results are shown in Table 3. The liver glycogen content of each group of mice in the embodiment and the comparative example was higher than that of the blank group, indicating that the composition of each group can increase the reserve of liver glycogen to varying degrees, among which the effect of embodiment 1 is the best, indicating that the composition prepared by the prescription and preparation method of the present invention can achieve the effect of relieving physical fatigue by increasing the reserve of liver glycogen.

[0088] Table 3 Liver glycogen content of mice in each group

[0089] Grouping Liver glycogen content (mg / g) Blank Group 9.56 Example 1 16.89 Comparative Example 1 12.78 Comparative Example 2 13.68 Comparative Example 3 11.21 Comparative Example 4 12.62 Comparative Example 5 13.74

[0090] 2.4 Blood lactate determination

[0091] 30 minutes after the last gavage of the 4 batches of mice, blood was collected from the inner canthus of the mice, and then they swam in water at a temperature of 30°C without weight for 10 minutes and then stopped. 20 μL of blood was collected before swimming, 20 μL of blood was collected immediately after swimming, and 20 μL of blood was collected again after resting for 20 minutes. The blood lactate was measured with a lactate meter, and the average value of the blood lactate content of each mouse was taken as the blood lactate content of the group of mice. The results are shown in Table 4. The blood lactate content of mice in each group of the embodiment and the comparative example was lower than that of the blank group, indicating that the composition of each group can improve the metabolic capacity of mice for lactic acid, among which the effect of Example 1 is the best, indicating that the composition prepared by the formula and preparation method of the present invention can quickly remove the blood lactate produced after high-load exercise to achieve the effect of relieving physical fatigue.

[0092] Table 4 Blood lactate content of mice in each group

[0093]

[0094] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Gynostemma pentaphyllum composition, characterized in that The invention comprises the following raw materials in parts by weight: 8-12 parts of gynostemma pentaphyllum, 8-12 parts of wolfberry, 6-10 parts of honeysuckle and 3-7 parts of orange peel.

2. The Gynostemma pentaphyllum composition according to claim 1, characterized in that The invention comprises the following raw materials in parts by weight: 9-11 parts of gynostemma pentaphyllum, 9-11 parts of wolfberry, 7-9 parts of honeysuckle and 4-6 parts of orange peel.

3. The method for preparing the Gynostemma pentaphyllum composition according to any one of claims 1 to 2, characterized in that: The following steps are involved: After extracting Gynostemma pentaphyllum, Lycium barbarum and Citrus aurantium with ethanol, the solid part is taken out and water is added to perform water extraction 1 to obtain water extract 1; Add water to honeysuckle to perform water extraction 2 to obtain water extract 2; The water extract 1 and the water extract 2 are mixed to obtain a Jiaogulan composition.

4. The preparation method according to claim 3, characterized in that: The mass volume ratio of the gynostemma pentaphyllum, wolfberry fruit and orange peel to ethanol is 1g:8-12mL.

5. The preparation method according to claim 3, characterized in that: The ethanol extraction step comprises: extracting at 38-42° C. for 30-38 minutes.

6. The preparation method according to claim 3, characterized in that: The mass volume ratio of the gynostemma pentaphyllum, wolfberry fruit and orange peel to water is 1g:18-22mL.

7. The preparation method according to claim 3, characterized in that: The step of water extraction 1 comprises: extracting at 55-65° C. for 28-32 minutes.

8. The preparation method according to claim 3, characterized in that: The mass volume ratio of the honeysuckle and water is 1g:18-22mL.

9. The preparation method according to claim 3, characterized in that: The step of water extraction 2 comprises: extracting at 55-65° C. for 28-32 minutes.

10. Use of the Gynostemma pentaphyllum composition according to any one of claims 1 to 2 or the preparation method according to any one of claims 3 to 9 in the preparation of a product for relieving physical fatigue.

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