Filtering and separating device and cell filtering and extracting method thereof

Abstract

The invention discloses a filtering and separating device and a cell filtering and extracting method thereof, and relates to the field of filtering and separating. The suspension to be filtered can be sucked into the liquid storage part through the negative pressure suction force of the pressure part, and then the suspension in the liquid storage part is pumped and filtered out from the filtering part so as to filter the suspension, so that the suspension can be extruded and filtered through the cooperation of the pressure part, the liquid storage part and the filtering part; the filtering efficiency and the filtering effect of the suspension can be improved, meanwhile, the suspension is prevented from being adhered and remained in the filtering part, and waste is reduced; the liquid storage part adopts the pointed dropper, and the filtering part adopts the filtering plug connected with the pointed end of the pointed dropper, so that the volume of the filtering part can be reduced, and therefore, even if residues exist in the filtering part during filtering, compared with a detachable filter screen matched with a filtering hole, the residual quantity can be greatly reduced, and the waste is further reduced.

Description

Technical Field

[0001] The invention belongs to the field of filtration separation, and in particular, relates to a filtration separation device and a cell filtration extraction method thereof. Background Art

[0002] Chinese patent CN221471033U discloses a first hard board body and a second hard board body, wherein the first hard board body is arranged on the upper side of the second hard board body, and a liquid inlet hole, a liquid outlet hole and a connecting pipe are arranged in the plate wall of the first hard board body, and the two side ends of the connecting pipe are respectively connected to the inner ends of the liquid inlet hole and the liquid outlet hole, and a filter hole is arranged in the middle of the connecting pipe, and the filter hole extends downward and passes through the first hard board body, and a filter screen is arranged in the filter hole; a liquid discard hole is arranged on the second hard board body, and the liquid discard hole extends in the up and down directions.

[0003] The above patent filters through the filter hole with a detachable filter. It uses a disposable replaceable filter to reduce the risk of filtration blockage. However, due to the certain viscosity of the suspension during filtration, the fluidity of the suspension is poor, resulting in low filtration efficiency. It is also easy to adhere to the surface of the filter membrane to form residues, which makes it impossible to filter partly, resulting in waste. Summary of the invention

[0004] In view of the problems in the related art, the present invention proposes a filtration separation device and a cell filtration extraction method thereof to overcome the above-mentioned technical problems existing in the existing related art.

[0005] In order to solve the above technical problems, the present invention is achieved through the following technical solutions:

[0006] The present invention is a filtering and separating device, comprising a pressure part, a liquid storage part and a filtering part which can be connected in sequence, wherein the pressure part can suck the suspension to be filtered into the liquid storage part by negative pressure suction, and then pump the suspension in the liquid storage part out of the filtering part to filter the suspension;

[0007] The pressure part includes a pressure regulating unit and a suction nozzle, wherein a pressure chamber is arranged inside the suction nozzle, and the pressure regulating unit can regulate the pressure in the pressure chamber; the liquid storage part includes a pointed dropper, and a pin groove is arranged in the opening at the top of the pointed dropper, and the bottom end of the suction nozzle is sealed and inserted in the opening at the top of the pointed dropper, and the outer ring of the bottom end of the suction nozzle is provided with a clamping locking component that can be clamped with the pin groove;

[0008] The filter part comprises a filter plug, a filter cavity is arranged inside the filter plug, a sealing interface communicating with the filter cavity is provided at the top of the filter plug, a filter hole is provided on the outer wall of the filter plug, and the filter plug is sealed and plugged with the bottom end of the pointed dropper through the sealing interface;

[0009] A push-and-separate component is also installed on one side of the pressure part, and the push-and-separate component includes an elastic lifting unit and a lifting sleeve. The lifting sleeve is slidably mounted on the outer ring of the suction nozzle, and the elastic lifting unit can drive the lifting sleeve to move up and down along the outer ring of the suction nozzle, and when the lifting sleeve moves downward along the outer ring of the suction nozzle, the lifting sleeve can first abut and retract the card-connecting locking component toward the inside of the suction nozzle, so that the card-connecting locking component releases the lock on the pointed dropper, and then abuts and moves the pointed dropper downward to separate the pointed dropper from the suction nozzle.

[0010] Furthermore, the pressure regulating unit includes a handle, the suction nozzle is fixedly installed at the bottom end of the handle, a lifting rod is slidably installed inside the handle, a pressure plate is fixedly installed at the bottom end of the lifting rod, a piston rod is fixedly installed at the bottom end of the lifting rod, the lower end of the piston rod extends into the interior of the pressure chamber, and a piston is fixedly installed at the bottom end of the piston rod.

[0011] Furthermore, a limit plate is fixedly installed inside the handle, a lifting slide plate located below the limit plate is fixedly installed at the lower end of the lifting rod, and a pressure spring sleeved on the outer ring of the piston rod is installed in contact with the bottom surface of the lifting slide plate.

[0012] Furthermore, a sealing groove is provided in the opening at the bottom end of the pointed dropper, a sealing ring is installed in the sealing groove, a guiding inclined surface is provided on the outer side of the bottom end of the sealing groove, and the pin groove is provided on the inner side of the lower end of the sealing groove.

[0013] Furthermore, the snap-on locking assembly includes a plurality of receiving grooves, which are circumferentially distributed and opened at the lower end of the suction nozzle. A pin-connecting column is slidably installed at the bottom end of the receiving groove, an elastic telescopic part is abutted against the inner end of the pin-connecting column, and an outer end of the pin-connecting column extends to the outside of the suction nozzle.

[0014] Furthermore, the elastic telescopic part includes a positioning rod, which is fixedly installed on the inner wall of the top end of the storage groove, and a telescopic slider is slidably inserted on the positioning rod. One end of the telescopic slider extends to the outside of the suction nozzle, and the other end of the telescopic slider is abutted against a return spring mounted on the outer ring of the positioning rod. The bottom end of the telescopic slider is connected to the top end of the pin-connecting column by a connecting strip.

[0015] Furthermore, a clamping ring is fixedly mounted on the outer ring of the bottom end of the pointed dropper, a guiding arc surface is arranged at the bottom end of the clamping ring, and a clamping groove capable of engaging with the clamping ring is arranged inside the filter plug.

[0016] Furthermore, the elastic lifting unit includes an outer shell, the outer shell is fixedly mounted on one side of the handle, a lifting shaft is slidably mounted inside the outer shell, a pressing slide cover is slidably mounted on the top of the outer shell, the top of the lifting shaft is fixedly connected to the pressing slide cover, and the bottom of the lifting shaft extends to the bottom of the outer shell and is fixedly connected to the lifting sleeve;

[0017] The upper and lower ends of the outer shell are respectively fixedly installed with a limit stop block and a bottom plate, the lifting shaft is fixedly installed with a lifting slider located below the limit stop block, and the lifting shaft is also equipped with a supporting spring abutting between the lifting slider and the bottom plate.

[0018] Furthermore, the elastic lifting unit also includes a limiting slide groove arranged at the bottom end of the handle, a limiting slide sleeve is slidably installed inside the limiting slide groove, the bottom end of the limiting slide sleeve is fixedly connected to the top end of the lifting slide sleeve, a lifting seat is fixedly installed on one side of the outer ring of the limiting slide sleeve, and the lifting seat is fixedly connected to the bottom end of the lifting shaft.

[0019] The present invention also discloses a cell filtration extraction method, the specific steps of which are as follows:

[0020] First, the bottom end of the suction nozzle is sealed and inserted into the opening at the top end of the pointed dropper, and the snap-fit ​​locking assembly is inserted into the pin groove to snap-fit ​​and lock the pointed dropper and the suction nozzle;

[0021] Then, the bottom end of the pointed dropper is inserted into the cell suspension, and the pressure chamber in the suction nozzle is adjusted to a negative pressure state through the pressure regulating unit, so that the pressure chamber sucks the cell suspension into the pointed dropper through negative pressure suction;

[0022] Then, the bottom end of the pointed dropper is inserted into the sealing interface at the top of the filter plug, so that the pointed dropper is sealed and connected with the filter plug, and then the pressure in the pressure chamber is increased by adjusting the pressure regulating unit, so that the pressure chamber squeezes and pumps the cell suspension in the pointed dropper into the inside of the filter plug, and filters the cell suspension through the filter holes on the outer wall of the filter plug, so that the cell suspension and the cells in it are filtered out through the filter holes, while the impurities in the cell suspension are isolated and retained in the filter plug;

[0023] After the filtration is completed, the lifting sleeve is driven by the elastic lifting unit to move downward along the outer circle of the suction nozzle. At this time, the lifting sleeve can first retract the snap-fit ​​locking assembly toward the inside of the suction nozzle, so that the snap-fit ​​locking assembly is separated from the pin groove to release the lock on the pointed dropper, and then the pointed dropper is moved downward to separate the pointed dropper from the suction nozzle, thereby realizing the rapid separation of the pressure part, the liquid storage part and the filtering part.

[0024] The present invention has the following beneficial effects:

[0025] 1. In the present invention, the suspension to be filtered can be sucked into the liquid storage part by the negative pressure suction of the pressure part, and then the suspension in the liquid storage part is pumped out from the filter part to filter the suspension, so that the suspension can be squeezed and filtered through the cooperation of the pressure part, the liquid storage part and the filter part. The filtration efficiency and filtration effect of the suspension can be improved through squeezing and pumping, and adhesion and residue in the filter part can be prevented, thereby reducing waste. The liquid storage part adopts a pointed dropper, and the filter part adopts a filter plug connected to the tip of the pointed dropper, which can reduce the volume of the filter part. Therefore, even if there is residue in the filter part during filtration, the residue amount can be greatly reduced compared with the filtration hole with a detachable filter screen, thereby further reducing waste.

[0026] 2. In the present invention, when the suction nozzle of the pressure part is plugged into the pointed dropper of the liquid storage part, the snap-on locking component at the bottom end of the suction nozzle can be synchronously plugged into the pin groove inside the pointed dropper, so that the snap-on locking component and the pin groove cooperate to snap-lock the pointed dropper and the suction nozzle, thereby improving the firmness of the connection between the suction nozzle and the pointed dropper, preventing the suction nozzle and the pointed dropper from being squeezed and separated under the action of the pumping pressure during the filtration process, and ensuring the normal filtration process; and when the suspension filtration is completed, the lifting sleeve can be driven by the elastic lifting unit to move downward along the outer circle of the suction nozzle. At this time, the lifting sleeve can first abut and retract the snap-on locking component toward the inside of the suction nozzle, so that the snap-on locking component is separated from the pin groove to release the lock of the pointed dropper, and then the pointed dropper is abutted and moved downward to separate the pointed dropper from the suction nozzle, thereby realizing the rapid separation of the pressure part from the liquid storage part and the filtration part, so as to facilitate the reuse of the pressure part.

[0027] 3. A sealing ring is installed in the opening of the pointed dropper in the present invention, which can improve the sealing performance when the pointed dropper is connected to the suction nozzle through the sealing ring, prevent air leakage at the connection between the pointed dropper and the suction nozzle, and ensure that there is sufficient pumping pressure in the filter chamber during suspension filtration. When the snap-on locking assembly is snapped into the pin groove, the snap-on locking assembly can squeeze and deform the sealing ring downward to squeeze the sealing ring against the joint between the pointed dropper and the suction nozzle, thereby further improving the sealing performance of the connection between the pointed dropper and the suction nozzle.

[0028] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the invention, the drawings required for describing the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the invention. For ordinary technicians in this field, they can also obtain drawings based on these drawings without paying creative work.

[0030] Figure 1This is one of the three-dimensional structural schematic diagrams of the filtering and separating device of the present invention;

[0031] Figure 2 For the present invention Figure 1 A local enlarged structural schematic diagram;

[0032] Figure 3 For the present invention Figure 1 A schematic diagram of the local enlarged structure at B;

[0033] Figure 4 For the present invention Figure 1 A schematic diagram of the local enlarged structure at C;

[0034] Figure 5 This is the second schematic diagram of the three-dimensional structure of the filtering and separating device of the present invention;

[0035] Figure 6 For the present invention Figure 5 A schematic diagram of the local enlarged structure at D;

[0036] Figure 7 The third schematic diagram of the three-dimensional structure of the filtering and separating device of the present invention;

[0037] Figure 8 For the present invention Figure 7 Schematic diagram of the local enlarged structure at E.

[0038] In the figure: 1. pressure part; 11. handle; 12. pressure plate; 13. lifting rod; 14. pressure spring; 15. suction nozzle; 16. piston; 17. pressure chamber; 18. piston rod; 19. lifting slide plate; 110. limit plate; 2. liquid storage part; 21. pointed dropper; 22. pin groove; 23. sealing groove; 24. sealing ring; 25. guide slope; 26. snap ring; 3. filter part; 31. filter plug; 32. sealing interface; 33. filter chamber; 34. Filter hole; 35, card slot; 4, card locking assembly; 41, storage slot; 42, positioning rod; 43, reset spring; 44, telescopic slider; 45, connecting strip; 46, pin-connected column; 5, push-release assembly; 51, press slide cover; 52, outer shell; 53, lifting shaft; 54, lifting sleeve; 55, limit stop block; 56, lifting slider; 57, support spring; 58, bottom plate; 59, limit slide groove; 510, lifting seat; 511, limit sleeve. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the invention to clearly and completely describe the technical solutions in the embodiments of the invention. Obviously, the described embodiments are only part of the embodiments of the invention, not all of the embodiments. Based on the embodiments in the invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the invention.

[0040] In the description of the present invention, it is necessary to understand that the terms "opening", "upper", "lower", "top", "middle", "inside" and the like indicating orientation or positional relationship are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the invention.

[0041] Embodiment 1

[0042] See also Figure 1-Figure 4 As shown, the present invention is a filtering and separating device, comprising a pressure part 1, a liquid storage part 2 and a filtering part 3 which can be connected in sequence, the pressure part 1 can suck the cell suspension to be filtered into the liquid storage part 2 by negative pressure suction, and then pump the cell suspension in the liquid storage part 2 out of the filtering part 3 to filter the cell suspension; the pressure part 1 comprises a pressure regulating unit and a suction nozzle 15, a pressure chamber 17 is arranged inside the suction nozzle 15, the pressure regulating unit can regulate the pressure in the pressure chamber 17, the liquid storage part 2 comprises a pointed dropper 21, a pin groove 22 is arranged in the opening at the top end of the pointed dropper 21, the bottom end of the suction nozzle 15 is sealed and inserted in the opening at the top end of the pointed dropper 21, and the outer ring of the bottom end of the suction nozzle 15 is provided with a snap-on locking component 4 which can be snap-connected with the pin groove 22; the filtering part 3 comprises a filter plug 31, a filter plug 31 is arranged inside The filter plug 31 is provided with a sealing interface 32 connected with the filter cavity 33 at the top, and a filter hole 34 is provided on the outer wall of the filter plug 31, and the filter plug 31 is sealed and plugged with the bottom end of the pointed dropper 21 through the sealing interface 32; a push-and-release assembly 5 is also installed on one side of the pressure part 1, and the push-and-release assembly 5 includes an elastic lifting unit and a lifting sleeve 54, and the lifting sleeve 54 is slidably sleeved on the outer ring of the suction nozzle 15, and the elastic lifting unit can drive the lifting sleeve 54 to move up and down along the outer ring of the suction nozzle 15, and when the lifting sleeve 54 moves downward along the outer ring of the suction nozzle 15, the lifting sleeve 54 can firstly abut and retract the clamping locking assembly 4 to the inside of the suction nozzle 15, so that the clamping locking assembly 4 releases the lock on the pointed dropper 21, and then abuts and moves the pointed dropper 21 downward, so that the pointed dropper 21 is separated from the suction nozzle 15;

[0043] The pressure part 1 is a recyclable component, while the liquid storage part 2 and the filter part 3 are in direct contact with the cell suspension during filtration. In order to ensure the purity of the cell suspension after filtration, the liquid storage part 2 and the filter part 3 are disposable components and need to be dismantled and discarded after use.

[0044] Specifically, when filtering the cells in the suspension, first, the bottom end of the suction nozzle 15 is sealed and inserted into the opening at the top of the pointed dropper 21, and at this time, the snap-fit ​​locking component 4 is inserted into the pin groove 22 to snap-fit ​​and lock the pointed dropper 21 and the suction nozzle 15; then, the bottom end of the pointed dropper 21 is inserted into the cell suspension, and then the pressure chamber 17 in the suction nozzle 15 is adjusted to a negative pressure state through the pressure regulating unit, so that the pressure chamber 17 sucks the cell suspension into the pointed dropper 21 through the negative pressure suction; then, the bottom end of the pointed dropper 21 is inserted into the sealing interface 32 at the top of the filter plug 31, so that the pointed dropper 21 is sealed and connected to the filter plug 31, and then the pressure in the pressure chamber 17 is increased by adjusting the pressure regulating unit. , so that the pressure chamber 17 squeezes and pumps the cell suspension in the pointed dropper 21 to the inside of the filter plug 31, and filters the cell suspension through the filter holes 34 on the outer wall of the filter plug 31, so that the cell suspension and the cells therein are filtered out through the filter holes 34, while the impurities in the cell suspension are isolated and retained in the filter plug 31; after the filtration is completed, the lifting sleeve 54 is driven by the elastic lifting unit to move downward along the outer ring of the suction nozzle 15, at which time the lifting sleeve 54 can first abut and retract the clamping locking component 4 toward the inside of the suction nozzle 15, so that the clamping locking component 4 is separated from the pin groove 22 to release the lock on the pointed dropper 21, and then the pointed dropper 21 is abutted and moved downward to separate the pointed dropper 21 from the suction nozzle 15;

[0045] In this embodiment, the pressure part 1, the liquid storage part 2 and the filter part 3 are used to squeeze and filter the cell suspension. By squeezing and pumping, the filtration efficiency and filtration effect of the cell suspension can be improved, and at the same time, cells are prevented from adhering to and remaining in the filter part 3, thereby reducing the waste of cells. The liquid storage part 2 adopts a pointed dropper 21, and the filter part 3 adopts a filter plug 31 connected to the tip of the pointed dropper 21, which can reduce the volume of the filter part 3. Therefore, even if cells remain in the filter part 3 during filtration, the residual amount of cells can be greatly reduced compared with the filtration of the cylindrical filter cartridge and the filter membrane, thereby further reducing the waste of cells. The pointed dropper 21 can be connected to the nozzle 15 by the clamping locking component 4 and the pin groove 22. The clamping and locking between them improves the firmness of the connection between the suction nozzle 15 and the pointed dropper 21, prevents the suction nozzle 15 and the pointed dropper 21 from being squeezed and separated under the pumping pressure during the filtration process, and ensures the normal filtration process; and when the cell suspension is filtered, the lifting sleeve 54 can be driven by the elastic lifting unit to move downward along the outer circle of the suction nozzle 15. At this time, the lifting sleeve 54 can first abut and retract the clamping locking component 4 to release the lock between the suction nozzle 15 and the pointed dropper 21, and then abut and move the pointed dropper 21 downward to separate the pointed dropper 21 from the suction nozzle 15, thereby realizing the rapid separation of the pressure part 1 from the liquid storage part 2 and the filtering part 3, so as to facilitate the reuse of the pressure part 1.

[0046] Embodiment 2:

[0047] See also Figure 1-Figure 8 As shown, the difference between this embodiment and the above embodiment is that the pressure regulating unit includes a handle 11, a suction nozzle 15 is fixedly installed at the bottom end of the handle 11, a lifting rod 13 is slidably installed inside the handle 11, a pressure plate 12 is fixedly installed at the bottom end of the lifting rod 13, a piston rod 18 is fixedly installed at the bottom end of the lifting rod 13, the lower end of the piston rod 18 extends into the interior of the pressure chamber 17, and a piston 16 is fixedly installed at the bottom end of the piston rod 18; a limit plate 110 is fixedly installed inside the handle 11, a lifting slide plate 19 located below the limit plate 110 is fixedly installed at the lower end of the lifting rod 13, and a pressure spring 14 sleeved on the outer ring of the piston rod 18 is installed in contact with the bottom surface of the lifting slide plate 19;

[0048] A sealing groove 23 is provided in the opening at the bottom end of the pointed dropper 21, a sealing ring 24 is installed in the sealing groove 23, a guiding slope 25 is provided on the outer side of the bottom end of the sealing groove 23, and a pin groove 22 is provided on the inner side of the lower end of the sealing groove 23; the snap-on locking assembly 4 includes a plurality of receiving grooves 41, the receiving grooves 41 are circumferentially distributed and opened at the lower end of the suction nozzle 15, a pin connection column 46 is slidably installed at the bottom end of the receiving groove 41, an elastic telescopic part is abutted against the inner end of the pin connection column 46, and an outer end of the pin connection column 46 extends to the outside of the suction nozzle 15.

[0049] The elastic telescopic member includes a positioning rod 42, which is fixedly mounted on the inner side wall at the top of the storage groove 41, and a telescopic slider 44 is slidably inserted on the positioning rod 42, one end of the telescopic slider 44 extends to the outside of the suction nozzle 15, and the other end of the telescopic slider 44 is abutted against a return spring 43 sleeved on the outer ring of the positioning rod 42, and the bottom end of the telescopic slider 44 is connected to the top end of the pin-connecting column 46 by a connecting strip 45;

[0050] A clamping ring 26 is fixedly mounted on the outer ring of the bottom end of the pointed dropper 21. A guiding arc surface is provided at the bottom end of the clamping ring 26. A clamping groove 35 capable of engaging with the clamping ring 26 is provided inside the filter plug 31.

[0051] The bottom surface of the outer end of the pin-joint column 46 is provided with a guiding arc surface, and the connecting strip 45 is an elastic strip. When the bottom end of the suction nozzle 15 is inserted into the opening at the top end of the pointed dropper 21, the guiding arc surface of the outer end of the pin-joint column 46 abuts against the inner side of the opening of the pointed dropper 21, and the pin-joint column 46 shrinks into the storage groove 41 under the abutment of the pointed dropper 21, and abuts the connecting strip 45 to deform, and then the pin-joint column 46 moves downward to the sealing groove 23, and the pin-joint column 46 moves outward under the action of the reset elastic force of the connecting strip 45. The pin-joining column 46 extends out and abuts against the inner wall of the sealing groove 23, so that the pin-joining column 46 can deform the sealing ring 24 in the sealing groove 23 along the guiding inclined surface 25 toward the joint between the suction nozzle 15 and the pointed dropper 21 during the subsequent downward movement, so as to improve the sealing performance of the sealing ring 24 on the joint between the suction nozzle 15 and the pointed dropper 21, until the pin-joining column 46 moves down to the position of the pin groove 22, and the pin-joining column 46 is inserted into the pin groove 22 under the elastic action of the connecting strip 45, so as to lock the suction nozzle 15 and the pointed dropper 21;

[0052] Then, the pressing plate 12 is pressed downward with the thumb to move it downward, so as to drive the lifting rod 13, the piston 16 and the piston rod 18 downward, so that the piston 16 squeezes and discharges the air in the pressure chamber 17, and the pressure spring 14 is squeezed and contracted at the same time, and then the bottom end of the pointed dropper 21 is inserted into the cell suspension, and the pressing plate 12 is released. At this time, the lifting rod 13, the piston 16 and the piston rod 18 are reset upward under the reset elastic force of the pressure spring 14, and the cell suspension is sucked into the pointed dropper 21; then, the bottom end of the pointed dropper 21 is plugged into the top of the filter plug 31. The sealing interface 32 is inserted into the clamping ring 26 on the pointed dropper 21 into the clamping groove 35 in the sealing interface 32 to improve the firmness of the clamping connection between the pointed dropper 21 and the filter plug 31. Finally, the pressing plate 12 is pressed downward by the thumb again to drive the lifting rod 13, the piston 16 and the piston rod 18 downward, so that the air pressure in the pressure chamber 17 gradually increases, so as to squeeze and pump the cell suspension in the pointed dropper 21 into the interior of the filter plug 31, and filter the cell suspension through the filter holes 34 on the outer wall of the filter plug 31.

[0053] Embodiment 3

[0054] See also Figure 1-Figure 3As shown, the difference between this embodiment and the above embodiment is that the elastic lifting unit includes an outer shell 52, the outer shell 52 is fixedly installed on one side of the handle 11, a lifting shaft 53 is slidably installed inside the outer shell 52, a pressing slide cover 51 is slidably installed on the top of the outer shell 52, the top of the lifting shaft 53 is fixedly connected to the pressing slide cover 51, the bottom end of the lifting shaft 53 extends to the bottom of the outer shell 52 and is fixedly connected to the lifting sleeve 54; the upper and lower ends of the inner shell 52 are respectively fixedly installed with a limited stop block 55 and a bottom plate 58, and the lifting A lifting slider 56 located below the limiting stop block 55 is fixedly mounted on the shaft 53, and a supporting spring 57 abutting between the lifting slider 56 and the bottom plate 58 is also mounted on the lifting shaft 53; the elastic lifting unit also includes a limiting slide groove 59 arranged at the bottom end of the handle 11, and a limiting sleeve 511 is slidably mounted inside the limiting slide groove 59, and the bottom end of the limiting sleeve 511 is fixedly connected to the top end of the lifting sleeve 54, and a lifting seat 510 is fixedly mounted on one side of the outer ring of the limiting sleeve 511, and the lifting seat 510 is fixedly connected to the bottom end of the lifting shaft 53;

[0055] When the cell suspension is filtered, the thumb is used to press and slide the pressing slide cover 51 downward, so that the pressing slide cover 51 drives the lifting shaft 53, the lifting seat 510, the limiting sleeve 511 and the lifting sleeve 54 to move downward. At the same time, the supporting spring 57 is squeezed and contracted. When the lifting sleeve 54 moves downward, the guiding arc surface of the top surface of the telescopic slider 44 moves the telescopic slider 44 toward the receiving groove 41. At the same time, the telescopic slider 44 drives the pin-connecting column 46 to shrink and move into the receiving groove 41 synchronously through the connecting strip 45, so that the pin-connecting column 46 is separated from the pin groove 22, and the lock between the suction nozzle 15 and the pointed dropper 21 is released. After that, as the lifting sleeve 54 continues to slide down, the lifting sleeve 54 abuts against the top of the pointed dropper 21 and moves the pointed dropper 21 downward to separate the pointed dropper 21 from the suction nozzle 15.

[0056] Embodiment 4

[0057] See also Figure 1-Figure 8 As shown, this embodiment discloses a cell filtration extraction method, and the specific steps are as follows:

[0058] First, the bottom end of the suction nozzle 15 is sealed and inserted into the opening at the top end of the pointed dropper 21, and the locking assembly 4 is inserted into the pin groove 22 to lock the pointed dropper 21 and the suction nozzle 15;

[0059] Then, the bottom end of the pointed dropper 21 is inserted into the cell suspension, and the pressure chamber 17 in the suction nozzle 15 is adjusted to a negative pressure state through the pressure regulating unit, so that the pressure chamber 17 sucks the cell suspension into the pointed dropper 21 through negative pressure suction;

[0060] Then, the bottom end of the pointed dropper 21 is inserted into the sealing interface 32 at the top of the filter plug 31, so that the pointed dropper 21 is sealed and connected with the filter plug 31, and then the pressure in the pressure chamber 17 is increased by adjusting the pressure regulating unit, so that the pressure chamber 17 squeezes and pumps the cell suspension in the pointed dropper 21 into the inside of the filter plug 31, and filters the cell suspension through the filter holes 34 on the outer wall of the filter plug 31, so that the cell suspension and the cells in it are filtered out through the filter holes 34, while the impurities in the cell suspension are isolated and retained in the filter plug 31;

[0061] After the filtration is completed, the lifting sleeve 54 is driven by the elastic lifting unit to move downward along the outer circle of the suction nozzle 15. At this time, the lifting sleeve 54 can first retract the snap-fit ​​locking assembly 4 toward the inside of the suction nozzle 15, so that the snap-fit ​​locking assembly 4 is separated from the pin groove 22 to release the lock on the pointed dropper 21, and then the pointed dropper 21 is moved downward to separate the pointed dropper 21 from the suction nozzle 15, thereby realizing the rapid separation of the pressure part 1 from the liquid storage part 2 and the filtering part 3.

[0062] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0063] The preferred embodiments of the invention disclosed above are only used to help explain the invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the invention, so that those skilled in the art can understand and use the invention well.

Claims

1. A filtering and separation device, characterized in that: It comprises a pressure part, a liquid storage part and a filtering part which can be connected in sequence, wherein the pressure part can suck the suspension to be filtered into the liquid storage part by negative pressure suction, and then pump the suspension in the liquid storage part out of the filtering part to filter the suspension; The pressure part includes a pressure regulating unit and a suction nozzle, wherein a pressure chamber is arranged inside the suction nozzle, and the pressure regulating unit can regulate the pressure in the pressure chamber; the liquid storage part includes a pointed dropper, and a pin groove is arranged in the opening at the top of the pointed dropper, and the bottom end of the suction nozzle is sealed and inserted in the opening at the top of the pointed dropper, and the outer ring of the bottom end of the suction nozzle is provided with a clamping locking component that can be clamped with the pin groove; The filter part comprises a filter plug, a filter cavity is arranged inside the filter plug, a sealing interface communicating with the filter cavity is provided at the top of the filter plug, a filter hole is provided on the outer wall of the filter plug, and the filter plug is sealed and plugged with the bottom end of the pointed dropper through the sealing interface; A push-and-separate component is also installed on one side of the pressure part, and the push-and-separate component includes an elastic lifting unit and a lifting sleeve. The lifting sleeve is slidably mounted on the outer ring of the suction nozzle, and the elastic lifting unit can drive the lifting sleeve to move up and down along the outer ring of the suction nozzle, and when the lifting sleeve moves downward along the outer ring of the suction nozzle, the lifting sleeve can first abut and retract the card-connecting locking component toward the inside of the suction nozzle, so that the card-connecting locking component releases the lock on the pointed dropper, and then abuts and moves the pointed dropper downward to separate the pointed dropper from the suction nozzle.

2. A filtering and separation device according to claim 1, characterized in that: The pressure regulating unit includes a handle, the suction nozzle is fixedly installed at the bottom end of the handle, a lifting rod is slidably installed inside the handle, a pressure plate is fixedly installed at the bottom end of the lifting rod, a piston rod is fixedly installed at the bottom end of the lifting rod, the lower end of the piston rod extends into the interior of the pressure chamber, and a piston is fixedly installed at the bottom end of the piston rod.

3. A filtering and separation device according to claim 2, characterized in that: A limit plate is fixedly installed inside the handle, a lifting slide plate located below the limit plate is fixedly installed at the lower end of the lifting rod, and a pressure spring sleeved on the outer ring of the piston rod is installed in contact with the bottom surface of the lifting slide plate.

4. A filtering and separation device according to claim 2, characterized in that: A sealing groove is arranged in the opening at the bottom end of the pointed dropper, a sealing ring is installed in the sealing groove, a guiding inclined surface is arranged on the outer side of the bottom end of the sealing groove, and the pin groove is arranged on the inner side of the lower end of the sealing groove.

5. A filtering and separation device according to claim 4, characterized in that: The snap-on locking assembly includes a plurality of receiving grooves, which are circumferentially distributed and opened at the lower end of the suction nozzle. A pin-connecting column is slidably installed at the bottom end of the receiving groove, an elastic telescopic part is abutted against the inner end of the pin-connecting column, and the outer end of the pin-connecting column extends to the outside of the suction nozzle.

6. A filtering and separation device according to claim 4, characterized in that: The elastic telescopic part includes a positioning rod, which is fixedly installed on the inner wall of the top end of the storage groove. A telescopic slider is slidably inserted on the positioning rod. One end of the telescopic slider extends to the outside of the suction nozzle. The other end of the telescopic slider is abutted against a return spring mounted on the outer ring of the positioning rod. The bottom end of the telescopic slider is connected to the top end of the pin-connecting column by a connecting strip.

7. A filtering and separation device according to claim 1, characterized in that: A clamping ring is fixedly mounted on the outer ring of the bottom end of the pointed dropper, a guiding arc surface is arranged at the bottom end of the clamping ring, and a clamping groove which can be engaged with the clamping ring is arranged inside the filter plug.

8. A filtering and separating device according to claim 6, characterized in that: The elastic lifting unit comprises an outer shell, the outer shell is fixedly mounted on one side of the handle, a lifting shaft is slidably mounted inside the outer shell, a pressing sliding cover is slidably mounted on the top of the outer shell, the top of the lifting shaft is fixedly connected to the pressing sliding cover, and the bottom of the lifting shaft extends to the bottom of the outer shell and is fixedly connected to the lifting sliding sleeve; The upper and lower ends of the outer shell are respectively fixedly installed with a limit stop block and a bottom plate, the lifting shaft is fixedly installed with a lifting slider located below the limit stop block, and the lifting shaft is also equipped with a supporting spring abutting between the lifting slider and the bottom plate.

9. A filtering and separating device according to claim 8, characterized in that: The elastic lifting unit also includes a limiting slide groove arranged at the bottom end of the handle, a limiting slide sleeve is slidably installed inside the limiting slide groove, the bottom end of the limiting slide sleeve is fixedly connected to the top end of the lifting slide sleeve, a lifting seat is fixedly installed on one side of the outer ring of the limiting slide sleeve, and the lifting seat is fixedly connected to the bottom end of the lifting shaft.

10. A cell filtration extraction method, using the filtration separation device according to any one of claims 1 to 9, characterized in that: The specific steps are as follows: First, the bottom end of the suction nozzle is sealed and inserted into the opening at the top end of the pointed dropper, and the snap-fit ​​locking assembly is inserted into the pin groove to snap-fit ​​and lock the pointed dropper and the suction nozzle; Then, the bottom end of the pointed dropper is inserted into the cell suspension, and the pressure chamber in the suction nozzle is adjusted to a negative pressure state through the pressure regulating unit, so that the pressure chamber sucks the cell suspension into the pointed dropper through negative pressure suction; Then, the bottom end of the pointed dropper is inserted into the sealing interface at the top of the filter plug, so that the pointed dropper is sealed and connected with the filter plug, and then the pressure in the pressure chamber is increased by adjusting the pressure regulating unit, so that the pressure chamber squeezes and pumps the cell suspension in the pointed dropper into the inside of the filter plug, and filters the cell suspension through the filter holes on the outer wall of the filter plug, so that the cell suspension and the cells in it are filtered out through the filter holes, while the impurities in the cell suspension are isolated and retained in the filter plug; After the filtration is completed, the lifting sleeve is driven by the elastic lifting unit to move downward along the outer circle of the suction nozzle. At this time, the lifting sleeve can first retract the snap-fit ​​locking assembly toward the inside of the suction nozzle, so that the snap-fit ​​locking assembly is separated from the pin groove to release the lock on the pointed dropper, and then the pointed dropper is moved downward to separate the pointed dropper from the suction nozzle, thereby realizing the rapid separation of the pressure part, the liquid storage part and the filtering part.

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