Microbial composition and application thereof

Abstract

The invention provides a microbial composition and application thereof, and belongs to the technical field of microbial utilization. The invention provides a microbial composition. The microbial composition comprises a healthy indigenous flora and a foreign specific flora which are subpackaged. The healthy indigenous flora disclosed by the invention is relatively good in internal coordination and balance, and functional microbial strains which are suitable for natural environmental conditions of local tobacco areas and disappear in farmlands can be introduced, so that a harmonious healthy soil flora is quickly constructed in local degraded soil; foreign specific flora is introduced, so that specific strains which disappear in the soil in tens of years or even hundreds of years are supplemented. Therefore, the healthy indigenous flora and the external specific flora can exert respective advantages, corresponding shortages are supplemented, the continuous cropping tobacco field soil with degraded biological functions is quickly repaired, and a method which is quick, economical, effective and easy to operate is provided for production.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial utilization, and in particular relates to a microbial combination and application thereof. Background Art

[0002] Tobacco is an important cash crop. Due to the high economic benefits of tobacco cultivation, tobacco farmers often carry out continuous cropping. Soil continuous cropping barriers have become an urgent problem to be solved in tobacco production. Microorganisms and their environment together constitute a complex microbiome, which plays an irreplaceable role in supporting ecosystem processes and functions. The microbiome provides a powerful tool for regulating soil microorganisms, promoting soil health and improving crop production, as well as environmental restoration. Species diversity strongly affects the function of microbial communities, and a single strain is unlikely to perform multiple expected functions. Compared with a single strain, a complex bacterial community can provide more functions related to soil nutrient bioavailability, soil resilience, plant nutrition and hormone balance.

[0003] At present, most artificial composite flora are composed of several bacteria, which are simple in species and unstable in function. It is easy to produce a phenomenon that a small difference in composition leads to a large difference in function. In addition, the development and utilization of artificial composite microbial agents such as EM and their bio-organic fertilizers are still limited in the restoration of tobacco continuous cropping obstacles, and there are relatively few technologies for the utilization of indigenous flora. Summary of the invention

[0004] The purpose of the present invention is to provide a microbial combination and application thereof. The present invention utilizes indigenous flora to effectively repair tobacco continuous cropping obstacles.

[0005] The present invention provides a microbial combination, including packaged healthy indigenous flora and foreign specific flora; the healthy indigenous flora includes fermented cultures of indigenous microorganisms captured in soil of a nature reserve adjacent to a tobacco-producing area or fermented cultures of indigenous microorganisms captured in organically cultivated fields adjacent to the tobacco-producing area for more than 10 years; the foreign specific flora includes at least one of Thomas flora, Pasteur flora and Japanese EM flora.

[0006] Preferably, the nature reserve is a virgin forest nature reserve; and the number of soil samples collected from the nature reserve adjacent to the tobacco-producing area is ≥3.

[0007] Preferably, the method for preparing the healthy indigenous flora comprises the following steps: mixing the indigenous microbial capture material in the soil of a nature reserve adjacent to the tobacco area with a carbon source and placing the mixture in a sterile open container, sealing the open container, and performing activation culture to obtain an activated product; mixing the activated product with water and performing aerobic fermentation to obtain a healthy indigenous flora.

[0008] Preferably, the volume of the indigenous microorganism capture material and the carbon source accounts for 2 / 3 to 4 / 5 of the volume of the open container.

[0009] Preferably, when the amount of indigenous microorganism capture materials is more than 2 parts, each part of the indigenous microorganism capture materials is subjected to activation culture and aerobic fermentation respectively, and then the products of aerobic fermentation are mixed in equal parts by weight.

[0010] The present invention also provides the use of the microbial combination described in the above scheme in repairing tobacco continuous cropping obstacles and / or promoting tobacco growth.

[0011] The present invention also provides a method for repairing tobacco continuous cropping obstacles and / or promoting tobacco growth, comprising the following steps: applying the healthy indigenous bacterial flora and the foreign specific bacterial flora in the microbial combination described in the above scheme to the tobacco field respectively.

[0012] Preferably, the application method includes root irrigation and / or application to soil.

[0013] Preferably, the healthy indigenous bacterial flora is diluted and then used for root irrigation, or the healthy indigenous bacterial flora is prepared into a solid bacterial agent and then applied to holes.

[0014] Preferably, preparing the healthy indigenous flora into a solid bacterial agent comprises: mixing the healthy indigenous flora with a solid raw material to obtain a solid bacterial agent; the solid raw material comprises biochar or decomposed organic fertilizer.

[0015] The present invention provides a microbial combination, including packaged healthy indigenous flora and foreign specific flora; the healthy indigenous flora includes fermented cultures of indigenous microorganisms captured in soil of a nature reserve adjacent to a tobacco area or fermented cultures of indigenous microorganisms captured in organically cultivated fields adjacent to a tobacco area for more than 10 years; the foreign specific flora includes at least one of Thomas flora, Pasteur flora and Japanese EM flora. Due to long-term natural selection, the healthy indigenous flora of the present invention has good internal coordination and balance, and can introduce functional microbial species that are suitable for the natural environmental conditions of the local tobacco area and have disappeared in the farmland, thereby quickly building a harmonious healthy soil flora in the local degraded soil; and the foreign specific flora introduces and supplements the specific species in the soil that have disappeared decades or even a hundred years ago. Therefore, the healthy indigenous flora and foreign specific flora of the present invention can give play to their respective advantages, make up for the corresponding shortcomings, and quickly repair the soil of the continuous tobacco field with degraded biological functions, providing a fast, economical, effective and easy-to-operate method for production. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0017] Figure 1 This is a picture of the field effect of the combination of healthy indigenous complex flora and foreign specific flora;

[0018] Figure 2 This is a comparison of the root morphology differences between the healthy indigenous composite flora and the foreign specific flora; CK: control, T1: combined with ETS microbial agent, T2: combined with EM microbial agent; specific root length (SRL), number of root tips per unit biomass (SRTA), number of forks per unit root length (FPRL), root diameter (Diameter);

[0019] Figure 3 This is the growth diagram of the repaired tobacco in Example 6;

[0020] Figure 4 This is the growth diagram of the control-treated tobacco in Comparative Example 3. DETAILED DESCRIPTION

[0021] The present invention provides a microbial combination, including packaged healthy indigenous flora and foreign specific flora; the healthy indigenous flora includes fermented cultures of indigenous microorganisms captured in soil of a nature reserve adjacent to a tobacco-producing area or fermented cultures of indigenous microorganisms captured in organically cultivated fields adjacent to the tobacco-producing area for more than 10 years; the foreign specific flora includes at least one of Thomas flora, Pasteur flora and Japanese EM flora.

[0022] The present invention aims to solve the problem of reduction of soil biodiversity and degradation of biological functions in tobacco fields caused by continuous tobacco cropping and long-term highly intensive production. When repairing the soil, healthy indigenous microbial flora and foreign specific microbial flora are jointly introduced to quickly restore the complex soil biological population, thereby achieving the purpose of effectively repairing the soil in continuously cropped tobacco fields with degraded biological functions.

[0023] In the present invention, the mixed culture is aerobic fermentation.

[0024] In the specific implementation process of the present invention, humus soil with rotten dead branches and fallen leaves is collected in the peripheral area of ​​the nature reserve adjacent to the key tobacco planting area to capture indigenous microorganisms in the nature reserve; the adjacent nature reserve and the tobacco planting area are roughly in the same environmental and climatic conditions, which are related to landforms, topography, soil, climate, longitude and latitude. According to the Chinese Tobacco Planting Regionalization (2009), there are currently five major tobacco leaf planting areas in my country, namely the Southwest Tobacco Planting Area, the Southeast Tobacco Planting Area, the Middle and Upper Reaches of the Yangtze River Tobacco Planting Area, the Northern Tobacco Planting Area, and the Huanghuai Tobacco Planting Area. The climate and soil of each area are significantly different, and they are further divided into 26 secondary tobacco planting areas. For example, Enshi and Xiangyang are both in the middle and upper reaches of the Yangtze River tobacco planting area (first-level area), and are both in the mountainous flue-cured tobacco and burley tobacco areas of Chongqing, western Hubei, and eastern Sichuan (secondary areas). However, the annual rainfall in the Enshi tobacco area is 1400-1500 mm, and in the Xiangyang tobacco area it is only 820-1100 mm. Therefore, the continuous cropping obstacles in the Enshi tobacco area are mainly manifested as continuous cropping diseases such as bacterial wilt, while the continuous cropping obstacles in the Xiangyang tobacco area are mainly manifested as severely restricted growth. Therefore, although both of these continuous cropping obstacles may be related to the imbalance of soil microbial populations, the specific mechanisms of action may be different. Therefore, the solutions are also different. For example, the Enshi tobacco area and the Xiangyang tobacco area should select the nature reserves in their respective areas to collect indigenous microorganisms; in one embodiment of the present invention, for the joint diseases, the Xiangyang tobacco area introduces foreign specific bacteria on the basis of the healthy indigenous microbiome and applies them in combination. In the specific implementation process of the present invention, the indigenous microbial captures in the soil of the nature reserve adjacent to the tobacco area are all captured using a portable indigenous microbiome ectopic trap, and sampling can be performed in the outer area of ​​the nature reserve; the portable indigenous microbiome ectopic trap refers to the Chinese patent application number CN 202411046945.7. The indigenous microbial capture obtained by the above-mentioned portable indigenous microbial group ectopic trap is a collection of bacteria, fungi, actinomycetes and protists. The various types of microorganisms therein have been domesticated naturally for a long time, and not only the components in the collection have reached a stable equilibrium state, but also a stable equilibrium state with the local natural environmental conditions, thereby forming a powerful and rich community that can form a functionally stable community, so the ecological function it exerts is also stable and efficient. Although the collected indigenous microbial captures seem to be random, the function is stable from the perspective of the collection as a whole. The healthy indigenous flora of the present invention is rich and stable. If the regional environment varies greatly, it is necessary to sample separately, reselect the healthy indigenous flora and configure the microbial combination, so there will be no problems of repeatability and low practicality for a specific area.

[0025] In the specific implementation of the present invention, the number of samples collected from the nature reserve adjacent to the tobacco area is ≥3, and further 3-5. In the specific implementation of the present invention, the nature reserve is a local nature reserve; the nature reserve includes a virgin forest nature reserve; the soil in the nature reserve adjacent to the tobacco area is collected from the dead branches and leaves layer under the broad-leaved forest, bamboo forest, grassland or perennial shrub forest. In one embodiment of the present invention, part of the soil in the nature reserve adjacent to the tobacco area is selected from the tree broad-leaved forest phase, with a large number of fallen leaves on the ground, and obviously rotten, with white mycelium produced, rotten leaves with white mycelium and 0-3cm of loose and humus-rich surface soil, and the number of collection points is 3-5; the other part is selected from the shrub broad-leaved forest phase, rotten leaves with white mycelium and 0-3cm of loose and humus-rich surface soil, and the number of collection points is 3-5. Due to long-term natural selection, the healthy indigenous flora of the present invention has good internal coordination and balance, and can introduce functional microbial species that are suitable for the natural environmental conditions of the local tobacco-growing areas and have disappeared in the farmland, thereby quickly building a harmonious healthy soil flora in the local degraded soil.

[0026] In one embodiment of the present invention, the virgin forest nature reserve is the virgin forest reserve of Dashui Forest Farm in Baokang County, Xiangyang City, Hubei Province, and mixed samples of rotten leaves in the litter layer under the forest and its humus-containing soil are collected.

[0027] In one embodiment of the present invention, the organic farming fields for more than 10 years are organic farming fields in Baokang County for more than 10 years, with no fertilizers or pesticides applied, and 0-15 cm soil layer samples are collected from 5 collection points.

[0028] In the specific implementation of the present invention, the preparation method of the healthy indigenous flora includes the following steps: mixing the indigenous microbial capture material and the carbon source in the soil of the nature reserve adjacent to the tobacco area and placing them in a sterile open container, sealing the open container, and performing activation culture to obtain an activated product; mixing the activated product with water and performing aerobic fermentation to obtain a healthy indigenous flora. In the specific implementation of the present invention, the carbon source is brown sugar or molasses; the mass ratio of the indigenous microbial capture material and the carbon source is 1:1; the open container includes a cleaned and disinfected brown wide-mouth bottle or clay pot; sealing the open container includes sealing with disinfected filter paper or rice paper; the volume of the indigenous microbial capture material and the carbon source accounts for 2 / 3 to 4 / 5 of the volume of the open container; the activation culture time is ≥7d. In the specific implementation process of the present invention, when the amount of indigenous microorganism capture is more than 2 parts, each part of the indigenous microorganism capture is respectively subjected to activation culture and aerobic fermentation, and then the products of aerobic fermentation are mixed in equal parts by mass; the volume ratio of the activated product to water is 1:10; the water is spring water, mineral water or tap water; when the water is tap water, it is also included to stand for 24 hours before use to remove the disinfectant in the water as much as possible; the aerobic fermentation time is 7 days; the aerobic fermentation is indoor fermentation.

[0029] In the specific implementation of the present invention, the foreign specific bacterial community is a commercial composite microbial agent with characteristics and a long history of development and use, including Jinshuitu black and white liquid bacterial agent and / or EM active liquid bacterial agent; the Jinshuitu black and white liquid bacterial agent is purchased from ETS (Tianjin) Biotechnology Development Co., Ltd.; the EM active liquid bacterial agent is purchased from Yimu Biotechnology (Nanjing) Co., Ltd. Since the Industrial Revolution, soil microbial resources with special functions may no longer exist in farmland soil due to fertilizers, pesticides, herbicides, and other industrial pollution. Foreign specific bacterial communities can introduce and supplement specific bacterial species in the soil that disappeared decades or even hundreds of years ago.

[0030] The present invention also provides the use of the microbial combination described in the above scheme in repairing tobacco continuous cropping obstacles and / or promoting tobacco growth.

[0031] In the present invention, the promoting tobacco growth includes promoting tobacco root growth.

[0032] The present invention also provides a method for repairing tobacco continuous cropping obstacles and / or promoting tobacco growth, comprising the following steps:

[0033] The healthy indigenous flora and the foreign specific flora in the microbial combination described in the above scheme are applied to the tobacco field respectively.

[0034] The present invention has no particular limitation on the order of applying the healthy indigenous flora and the foreign specific flora.

[0035] In the specific implementation process of the present invention, the application method includes root irrigation and / or application to the soil; the application to the soil includes strip application or hole application.

[0036] In the specific implementation process of the present invention, the healthy indigenous bacterial flora is diluted and then irrigated to the roots, or the healthy indigenous bacterial flora is prepared into a solid bacterial agent and then applied to the soil;

[0037] In one embodiment of the present invention, the dilution multiple of the healthy indigenous flora is 10 times; the foreign specific flora is diluted according to the dilution multiple in the instructions and then irrigated; the volume ratio of the 10-fold dilution of the healthy indigenous flora and the dilution of the foreign specific flora is 1:1; the 10-fold dilution of the healthy indigenous flora and the dilution of the foreign specific flora are respectively irrigated in the same tobacco planting hole; the irrigation volume of the 10-fold dilution of the healthy indigenous flora and the dilution of the foreign specific flora in each tobacco planting hole is 250 ml, respectively, as root-fixing water. To ensure the effect, irrigate the roots again according to the above method 15 to 20 days after transplanting.

[0038] In another embodiment of the present invention, the healthy indigenous flora is prepared into a solid bacterial agent and applied in holes or strips in the soil; the foreign specific flora is applied according to the product instructions.

[0039] In the specific implementation of the present invention, preparing the healthy indigenous flora into a solid microbial agent includes: mixing the healthy indigenous flora and solid raw materials, culturing, and obtaining a solid microbial agent; the solid raw materials include biochar or decomposed organic fertilizer; in the specific implementation of the present invention, the mass ratio of the composite indigenous microbial agent to other raw materials is 1: (1-5). In the specific implementation of the present invention, the biochar is prepared from plant materials; the plant materials include one or more of straw, rice husks, branches and wood waste, and further rice husks; the particle size of the biochar is 0.5-1mm; the decomposed organic fertilizer is high-quality decomposed organic fertilizer; the high-quality decomposed organic fertilizer includes earthworm compost, earthworm castings and / or insect sand. In the specific implementation of the present invention, the culturing time is 7 days; the culturing temperature is room temperature; the culturing includes composting culturing; the pile is gently turned every 2-3 days to ensure oxygen supply; the solid microbial agent is used as a solid soil conditioner, and the application amount is 20-50 kg / mu. In one embodiment of the present invention, the solid bacterial agent is applied together with other organic fertilizers during land preparation, fertilization and ridge formation 5 to 7 days before tobacco transplanting, or applied in holes during transplanting.

[0040] The microbial combination of the present invention can quickly repair tobacco continuous cropping farmland soil with degraded biological functions, can achieve good and stable repair effects, and is easy to apply. Combined with rooting water irrigation, it can save labor and water resources.

[0041] In order to further illustrate the present invention, a microbial combination and application thereof provided by the present invention are described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0042] Example 1

[0043] 1) Healthy indigenous flora preparation

[0044] In the original forest reserve of Dashui Forest Farm in Baokang County, Xiangyang, Hubei Province, we selected the arbor broad-leaved forest phase, where there were many fallen leaves on the ground, which were obviously rotten and had white mycelium produced. We collected the rotten fallen leaves with white mycelium and the surface soil with a thickness of 0-3 cm and rich in humus. We collected 3 to 5 points, mixed them into one sample, and marked it as BK01. According to the above method, we selected the shrub broad-leaved forest phase, collected the rotten fallen leaves with white mycelium and the surface soil with a thickness of 0-3 cm and rich in humus. We collected 3 to 5 points, mixed them into one sample, and marked it as BK02. In Baokang County, we selected organic farming plots with a history of more than 10 years (without the application of chemical fertilizers and pesticides), collected 0 to 15 cm soil layer samples (5-point mixed samples), and marked the samples as BK03. They were placed in pre-sterilized stainless steel square trays, and the sample laying thickness was about 1 cm. On the mixed sample, a disposable corn starch lunch box with microporous hollow bottom that has been cleaned and disinfected is placed, filled with pre-steamed organic brown rice, filling 2 / 3 of the height, leaving 1 / 3 of the gap, and the lunch box is covered with sterilized gauze and fixed with a sterilized rubber band. After the above operation is completed, it is placed in the constant temperature room of the indigenous microbiome ectopic trap for culture. The portable indigenous microbiome ectopic trap is placed in the car and brought back indoors, and the battery is charged once every 12 hours. After 5 to 7 days of culture, a large number of white and gray colonies are observed on the rice, and the collection process is completed (see Chinese patent application number CN202411046945.7 for details). The captured sample is fully mixed with brown sugar 1:1 (by mass) and placed in a cleaned and disinfected brown bottle for culture for about 1 week.

[0045] Healthy indigenous flora: Gently stir and mix BK01, BK02, and BK03 after 1 week of indoor culture, take 10ml of each and mix them, gently stir and mix, then add 300ml of mountain spring water or mineral water, gently stir and mix, put into a sterilized 500ml brown bottle, seal with sterilized filter paper, and culture indoors for 1 week, gently shake once a day to ensure aerobic conditions. The above composite bacterial agent is diluted 10 times with water and can be directly irrigated in the tobacco planting hole.

[0046] Indigenous solid composite bacterial agent: Mix the above-mentioned mixed bacterial agent that has been cultured aerobically for about one week with plant-derived biochar or high-quality decomposed organic fertilizer (such as earthworm compost, worm sand, etc.) in a mass ratio of 1:1 to 5. Cultivate it for about one week. It can be used as a solid soil conditioner and directly applied to the soil by hole application or strip application. The dosage per mu is 20 to 50 kg.

[0047] 2) Selection of foreign specific bacterial flora

[0048] Based on the results of a series of field plot tests from 2022 to 2023, in 2024, Jinshuitu Black and White Liquid (ETS, Jinshuitu Black and White Liquid) or EM compound bacterial agent will be selected as the foreign specific bacterial agent product for joint use in production to carry out field application tests.

[0049] Example 2: Use of healthy indigenous flora and ETS composite bacterial agent as rooting water

[0050] Select the biological function degraded continuous cropping tobacco fields in Yantangchong Village, Liangyu Township, Baokang County. After fertilizing, ridge-building and transplanting tobacco seedlings, the 10-fold dilution of the healthy indigenous flora prepared in Example 1 and the 200-fold dilution of the purchased ETS compound bacterial agent are used as rooting water to irrigate the soil at the roots of the tobacco seedlings. 250 ml of each of the two bacterial agents are irrigated (Note: in the same tobacco planting hole, 250 ml of the 10-fold dilution of the prepared healthy indigenous flora and 250 ml of the 200-fold dilution of the ETS compound bacterial agent prepared according to its instructions are irrigated successively). To ensure the effect, 15 to 20 days after transplanting, irrigate the roots again according to the above method (Note: the dosage is the same as above). In 2024, in the tobacco fields of Yantangchong Village, Liangyu Township, Baokang County, where the continuous cropping obstacles in previous years seriously affected the normal growth of tobacco, the application demonstration was 20 acres. The plants have well-developed root systems and strong vitality, and the continuously cropped degraded soil has been basically repaired.

[0051] Example 3: Use of healthy indigenous flora and EM bacterial agent as rooting water

[0052] Except that the ETS compound bacterial agent was replaced by the EM bacterial agent, the rest was the same as Example 2, and the EM bacterial agent was prepared and used according to the instructions.

[0053] Comparative Example 1

[0054] In 2022, in the field plots, foreign composite microbial agents (ETS composite agents or EM agents) and clean water (blank control) were used together as rooting water. From the application effect, some plots had significant effects, while some plots had insignificant effects. The experiment in the same field plot in 2023 showed that the method of Example 2 was used to combine healthy indigenous flora with foreign ETS composite agents, and the multi-point display effect was obvious. Therefore, in 2024, a large-scale application demonstration test of the combination of healthy indigenous flora and foreign composite microbial agents was carried out. The results showed that after treatment, the root system of the plants was well-developed and vigorous, and the continuous cropping degraded soil was basically repaired. The average height of the tobacco plants after topping was about 105 cm, and the average height of the control (Comparative Example 3) after topping was about 78 cm, an increase of 34.6%.

[0055] See Example 2, Example 3 and Comparative Example 1 for a comparison of the field effects of the 2022 test. Figure 1(Photo taken on August 20, 2024). Figure 1 It can be seen that no matter whether healthy indigenous flora is used in combination with ETS or EM, the growth of tobacco in the field is significantly better than that of the clear water control without the application of healthy indigenous flora.

[0056] Example 4: Combination of indigenous solid composite bacterial agent and EM composite bacterial flora

[0057] Select the tobacco fields with biological function degradation in Yantangchong Village, Liangyu Township, Baokang County. When transplanting tobacco seedlings, mix 20kg / mu of the above-mentioned solid composite bacterial agent (rice husk biochar-based) with 20kg of surface fine soil and evenly apply it to the tobacco planting holes of 1 mu. Dilute the purchased EM composite bacterial agent active solution 200 times according to its instructions and irrigate the soil at the roots of the tobacco seedlings as rooting water, irrigating 500ml per tobacco seedling. To ensure the effect, irrigate the roots once with 200 times diluted EM composite bacterial agent active solution 15 to 20 days after transplanting.

[0058] Example 5: Indigenous solid composite bacterial agent is used in combination with ETS composite bacterial agent.

[0059] Except that the EM bacterial agent was substituted for the ETS compound bacterial agent, the rest was the same as in Example 4. The ETS compound bacterial agent was prepared and used according to the instructions.

[0060] Comparative Example 2

[0061] The application of the indigenous solid composite bacterial agent was omitted, and the rest was the same as in Example 4.

[0062] For a comparison of the field effects of Example 4, Example 5 and Comparative Example 2, see Figure 2 (Sampled on August 20, 2024). In 2024, in Yantangchong Village, Liangyu Township, Baokang County, where continuous cropping obstacles in previous years seriously affected the normal growth of tobacco, 20 acres of tobacco fields were applied for demonstration. The plants in Examples 4 and 5 had well-developed root systems and strong vitality, and the continuous cropping degraded soil was basically repaired. Compared with the control group (Comparative Example 2), the specific root length (SRL) of the tobacco root system, the number of root tips per unit biomass (SRTA) and the number of forks per unit root length (FPRL) were increased, and the root diameter (Diameter) was reduced.

[0063] Example 6: Combination of indigenous solid composite bacterial agent and ETS composite bacterial agent

[0064] Select the tobacco fields with biological function degradation in Yantangchong Village, Liangyu Township, Baokang County. When transplanting tobacco seedlings, mix 20kg / mu of the above-mentioned solid composite bacteria agent (rice husk biochar-based) with 20kg of surface fine soil and evenly apply it to the tobacco planting holes of 1 mu. The purchased 200-fold diluted ETS composite bacteria agent was used as rooting water to irrigate the soil at the roots of the tobacco seedlings, and 500ml was irrigated for each tobacco seedling. To ensure the effect, irrigate the roots again with 200-fold diluted ETS composite bacteria agent 15 to 20 days after transplanting.

[0065] Comparative Example 3

[0066] The application of the indigenous solid composite bacterial agent was omitted, and the rest was the same as in Example 6.

[0067] For a comparison of the field effects of Example 6 and Comparative Example 3, see Figure 3 and Figure 4 In 2024, in Yantangchong Village, Liangyu Township, Baokang County, where continuous cropping obstacles seriously affected the normal growth of tobacco in previous years, 60 mu of tobacco fields were applied for demonstration. The plants in Example 6 had well-developed root systems and strong vitality, and the continuous cropping degraded soil was basically repaired. After the soil was repaired by the combined use of bacteria, the topped plant height of the tobacco plant was 100-110 cm, while the topped plant height of the control (Comparative Example 3) was only 66-91 cm.

[0068] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A microbial combination, characterized in that: Including packaged healthy indigenous flora and foreign specific flora; The healthy indigenous flora includes fermented cultures of indigenous microorganisms captured in soil of nature reserves adjacent to tobacco-producing areas or fermented cultures of indigenous microorganisms captured in organically cultivated fields adjacent to tobacco-producing areas for more than 10 years; The foreign specific bacterial group includes at least one of the Thomas bacterial group, the Pasteur bacterial group and the Japanese EM bacterial group.

2. The microbial combination according to claim 1, characterized in that The nature reserve is a virgin forest nature reserve; the number of soil samples collected from the nature reserve adjacent to the tobacco area is ≥3.

3. The microbial combination according to claim 1, characterized in that The method for preparing the healthy indigenous flora comprises the following steps: The indigenous microorganisms captured from the soil of a nature reserve adjacent to a tobacco-producing area are mixed with a carbon source and placed in a sterile open container. After the open container is sealed, activation culture is performed to obtain an activated product; the activated product is mixed with water and aerobic fermentation is performed to obtain a healthy indigenous flora.

4. The microbial combination according to claim 3, characterized in that The volume of the indigenous microorganism capture material and the carbon source occupies 2 / 3 to 4 / 5 of the volume of the open container.

5. The microbial combination according to claim 3, characterized in that: When the amount of indigenous microorganism capture materials is more than 2, each portion of the indigenous microorganism capture materials is subjected to activation culture and aerobic fermentation respectively, and then the products of aerobic fermentation are mixed in equal parts by mass.

6. Use of the microbial combination according to any one of claims 1 to 5 in repairing tobacco continuous cropping obstacles and / or promoting tobacco growth.

7. A method for repairing tobacco continuous cropping obstacles and / or promoting tobacco growth, characterized in that: The following steps are involved: The healthy indigenous flora and the foreign specific flora in the microbial combination of claim 1 are applied to tobacco fields respectively.

8. The method according to claim 7, characterized in that The application methods include root irrigation and / or application to soil.

9. The method according to claim 8, characterized in that The healthy indigenous bacterial flora is diluted and then used for root irrigation or the healthy indigenous bacterial flora is prepared into a solid bacterial agent and then applied to holes.

10. The method according to claim 9, characterized in that Preparing the healthy indigenous bacterial flora into a solid bacterial agent comprises: mixing the healthy indigenous bacterial flora with a solid raw material to obtain a solid bacterial agent; the solid raw material comprises biochar or decomposed organic fertilizer.

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