Porous microporous plate device with high-throughput gene detection

Abstract

The invention relates to the technical field of gene detection hole microwell plates, in particular to a porous microwell plate device with high-throughput gene detection, which comprises a plate body, a plurality of reaction holes are formed in the top of the plate body, the reaction holes are distributed in a rectangular shape, a storage box is slidably mounted in the plate body, and the storage box is connected with the plate body. The multiple storage boxes are located at the front ends of each row of reaction holes, electrophoresis mechanisms are installed between the reaction holes and the storage boxes, heating mechanisms are installed at the bottoms of the reaction holes, limiting assemblies are installed in the plate body, first channels are formed in the front ends of the reaction holes, and storage cavities are formed in the storage boxes; and a plurality of second channels are formed in the front end of the storage box and communicate with the storage cavity. According to the method, DNA can be independently purified, the purified DNA can be effectively separated, the experimental process is simplified, the detection flux and the result accuracy are improved, and high-flux gene detection application is facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of gene detection microplates, and in particular to a multi-hole microplate device with high-throughput gene detection. Background Art

[0002] With the development of society, genetic testing plays a critical role in many fields such as life sciences, medical diagnosis, and genetic research. It can deeply analyze the genetic information of individuals, help scientists understand the relationship between genes and diseases, and provide a strong basis for early diagnosis and precise treatment of diseases. In the medical field, genetic testing can be used to screen for genetic diseases such as cystic fibrosis and Huntington's disease, allowing doctors to formulate intervention measures in advance to reduce the risk of disease or delay the progression of the disease.

[0003] In genetic testing, a microplate is an experimental device with multiple tiny reaction wells. Its main function is to provide an independent reaction space during the genetic testing process so that multiple samples or multiple reaction conditions can be tested simultaneously. In specific usage, various reaction reagents, such as primers, enzymes, substrates, etc., are usually added to the reaction wells of the microplate, and then the DNA samples or other biological samples to be tested are added to different reaction wells respectively. By utilizing the high-throughput characteristics of the microplate, a large number of samples can be processed in parallel in one experiment, greatly improving the experimental efficiency.

[0004] However, microplates have certain limitations when testing genes because they cannot purify DNA by themselves. DNA purification is an important step in separating large and small fragments in DNA samples and is indispensable in the genetic testing process. Since microplates lack this function, other specialized equipment is needed for purification operations. This process not only increases the cost of experimental equipment, but also makes the experimental process complicated and cumbersome. Against the background of the growing demand for high-throughput testing, frequent transfer of samples between microplates and other purification equipment will extend the experimental cycle and make it difficult to achieve truly high-throughput testing. Moreover, samples are easily contaminated or lost during the transfer process, affecting the accuracy and reliability of the test results. Summary of the invention

[0005] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a multi-well microplate device with high-throughput gene detection.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a multi-porous microplate device with high-throughput gene detection, comprising a plate body, a reaction hole is opened on the top of the plate body, and the reaction holes are distributed in a plurality in a rectangular shape, a storage box is slidably installed inside the plate body, and the storage box has a plurality of locations at the front end of each row of reaction holes, an electrophoresis mechanism is installed between the reaction holes and the storage box, a heating mechanism is installed at the bottom of the reaction holes, a limiting component is installed inside the plate body, a first channel is opened at the front end of the reaction holes, a storage cavity is opened inside the storage box, a second channel is opened at the front end of the storage box and is connected to the storage cavity, there are a plurality of second channels, and the first channel and the second channel are connected to each other;

[0007] The electrophoresis mechanism includes a power supply module, an annular negative electrode block and a straight positive electrode block. The power supply module is fixedly installed inside the plate body, the annular negative electrode block is fixedly installed on the inner wall of the reaction hole, and the straight positive electrode block is fixedly installed on the inner wall of the storage cavity.

[0008] Further: a first power line is connected between the power supply module and the annular negative electrode block, and a second power line is connected between the power supply module and the straight positive electrode block.

[0009] Further: the heating mechanism includes a heating wire and a third power line, the heating wire is fixedly installed inside the plate body, the heating wire is located at the bottom of each row of reaction holes, and the third power line is connected between the power supply module and the heating wire.

[0010] Further: the limit assembly includes an installation groove, a moving block, a connecting block, a limit block and a limit groove, the installation groove is opened inside the plate body, the moving block is fixedly connected to one side of multiple storage boxes, the moving block is located inside the installation groove, the connecting block has two sliding installations inside the installation groove, the limit block has multiple fixed connections to the opposite surfaces of the two connecting blocks, the limit groove has multiple openings on the upper and lower sides of the moving block, and the limit block is located inside the limit groove.

[0011] Further: the limiting assembly also includes a push block and a spring, the push block is fixedly connected to one side of the two connecting blocks, the spring has two inner walls fixedly installed on the mounting groove, and the push block is fixedly connected to one end of the spring.

[0012] Further: a handle is fixedly connected to the outer wall of the moving block.

[0013] The present invention has the following beneficial effects:

[0014] 1. Compared with the prior art, by setting a reaction hole, a storage cavity, a power supply module, an annular cathode block, a straight cathode block, a first power line, a second power line, a heating wire and a third power line, first fill the reaction hole and the storage cavity with agarose gel of a suitable concentration, determine the agarose concentration according to the experimental requirements to regulate the gel pore size, and then place the DNA sample inside the reaction hole and on top of the agarose gel. Then start the power supply module, and power the annular cathode block and the straight cathode block through the first power line and the second power line to form an electric field. At the same time, the power supply module powers the heating wire through the third power line to increase the temperature. DNA molecules migrate to the storage cavity under the action of the electric field, small DNA fragments enter the storage cavity, and large DNA fragments remain in the reaction hole to achieve DNA purification. The present invention simplifies the experimental process, shortens the experimental cycle, improves the throughput of genetic testing, reduces costs and reduces the risk of sample contamination, and improves the accuracy and reliability of the test results.

[0015] 2. Compared with the prior art, by setting the first channel, the second channel, the installation groove, the moving block, the connecting block, the limiting block, the limiting groove, the pushing block, the spring and the pull handle, after the electrophoresis separation is completed, the pushing block is pushed to overcome the elastic force of the spring and drive the connecting block to move at the same time, so that the limiting block moves out of the limiting groove, and then the pull handle is pulled to drive the moving block to move, and the connecting block slides in the installation groove, so that the first channel and the second channel are misaligned and disconnected to prevent small fragments of DNA from flowing back. The present invention realizes the effective separation of purified DNA, ensures that subsequent detection is carried out in a pure and accurate sample environment, further improves the credibility of gene detection results, and is conducive to the effective application of gene detection technology in high-throughput detection scenarios. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0017] Figure 2 It is a schematic diagram of the explosion structure of the present invention;

[0018] Figure 3 for Figure 2 The enlarged structural diagram at A in the middle;

[0019] Figure 4 It is a schematic diagram of the cross-sectional structure of the present invention;

[0020] Figure 5 for Figure 4 The enlarged structural diagram at B in the middle;

[0021] Figure 6 It is an enlarged structural schematic diagram of the storage box and the moving block in the present invention.

[0022] Legend:

[0023] 1. Plate; 2. Reaction hole; 201. First channel; 3. Storage box; 301. Storage cavity; 302. Second channel; 4. Electrophoresis mechanism; 401. Power supply module; 402. Annular negative electrode block; 403. Straight positive electrode block; 404. First power line; 405. Second power line; 5. Heating mechanism; 501. Heating wire; 502. Third power line; 6. Limiting assembly; 601. Mounting groove; 602. Moving block; 603. Connecting block; 604. Limiting block; 605. Limiting groove; 606. Pushing block; 607. Spring; 7. Handle. DETAILED DESCRIPTION

[0024] Reference Figure 1-6 The present invention provides a multi-well microplate device with high-throughput gene detection, comprising a plate body 1, a reaction well 2 is opened on the top of the plate body 1, and the reaction wells 2 are arranged in a rectangular shape. A storage box 3 is slidably installed inside the plate body 1, and the storage box 3 is located at the front end of each row of reaction wells 2. An electrophoresis mechanism 4 is installed between the reaction wells 2 and the storage box 3. A heating mechanism 5 is installed at the bottom of the reaction wells 2. A limiting component 6 is installed inside the plate body 1. A first channel 201 is opened at the front end of the reaction well 2. The storage box 3 is provided with a plurality of storage boxes. A storage cavity 301 is provided inside, and a second channel 302 is provided at the front end of the storage box 3 and is connected to the storage cavity 301. There are multiple second channels 302, and the first channel 201 and the second channel 302 are connected to each other; the electrophoresis mechanism 4 includes a power supply module 401, an annular negative electrode block 402 and a straight positive electrode block 403. The power supply module 401 is fixedly installed inside the plate body 1, the annular negative electrode block 402 is fixedly installed on the inner wall of the reaction hole 2, and the straight positive electrode block 403 is fixedly installed on the inner wall of the storage cavity 301.

[0025] Before performing gene detection, agarose gel of appropriate concentration is first filled in the reaction hole 2 and the storage chamber 301, and the agarose concentration is selected according to the size of the DNA fragment to be separated (the pore size of the low-concentration gel is larger, and the pore size of the high-concentration agarose gel is smaller). Then the DNA sample is placed inside the reaction hole 2 and on the top of the agarose gel. Then the power supply module 401 is started, and the current reaches the annular cathode block 402 through the first power line 404, and reaches the straight cathode block 403 through the second power line 405, forming an electric field between the reaction hole 2 and the storage chamber 301. Since the DNA molecule carries a negative charge (its phosphate group is ionized under physiological pH conditions and carries a negative charge), under the action of the electric field, the DNA molecule will move to the anode (the storage chamber 301 where the straight cathode block 403 is located), and the small fragment DNA can migrate to the storage chamber 301 faster through the pores of the agarose gel due to its relatively small molecular weight and large migration rate, while the large fragment DNA is left in the reaction hole 2 due to the difficulty of migration, thereby achieving the purification and separation of DNA.

[0026] A first power line 404 is connected between the power supply module 401 and the annular negative electrode block 402 , and a second power line 405 is connected between the power supply module 401 and the straight positive electrode block 403 .

[0027] The first power line 404 and the second power line 405 serve as channels for current transmission, stably transmitting the electric energy generated by the power supply module 401 to the annular negative electrode block 402 and the straight positive electrode block 403, ensuring the stable formation of the electric field, providing a continuous and uniform driving force for DNA electrophoresis, enabling DNA molecules to migrate in the expected direction and rate, and ensuring the smooth progress of the DNA purification process.

[0028] The power supply module 401 first provides power through the internal battery, and converts the input AC power into stable DC power through the internal circuits and components, such as transformers, filters, etc., and adjusts it to a voltage value suitable for the various components of the device, providing a stable power supply for the entire porous microplate device to ensure that each component can work normally. After conversion and adjustment, the DC power will be distributed to the corresponding output port through the lines and connection points inside the power supply module 401. The electric energy is transmitted to the annular negative electrode block 402 through the first power line 404 to provide the required electric field force basis for the electrophoresis process in the reaction hole 2; at the same time, the electric energy is transmitted to the straight positive electrode block 403 through the second power line 405, so that the corresponding electric field is also formed in the storage cavity 301, thereby establishing a complete electrophoresis electric field loop between the reaction hole 2 and the storage cavity 301 to achieve electrophoretic separation of DNA samples. The third power line 502 provides electric energy for the heating wire 501, so that the heating wire 501 heats up, thereby increasing the temperature at the bottom of the reaction hole 2 and accelerating the electrophoresis process.

[0029] The heating mechanism 5 includes a heating wire 501 and a third power line 502 . The heating wire 501 is fixedly installed inside the plate body 1 . The heating wire 501 is located at the bottom of each row of reaction holes 2 . The third power line 502 is connected between the power supply module 401 and the heating wire 501 .

[0030] During the electrophoresis process, the power supply module 401 supplies power to the heating wire 501 through the third power line 502. The heating wire 501 generates heat, and the heat is transferred to the agarose gel and the sample in the reaction hole 2. The increased temperature can reduce the viscosity of the agarose gel and increase the migration rate of the DNA molecules in the gel, thereby speeding up the electrophoresis process, shortening the experimental time, and improving the detection efficiency. At the same time, the increase in temperature also helps some enzyme reactions or biochemical reactions to proceed during the electrophoresis process, which is beneficial to improving the accuracy and reliability of genetic testing.

[0031] The limiting assembly 6 includes an installation groove 601, a moving block 602, a connecting block 603, a limiting block 604 and a limiting groove 605. The installation groove 601 is opened inside the plate body 1, the moving block 602 is fixedly connected to one side of multiple storage boxes 3, the moving block 602 is located inside the installation groove 601, the connecting block 603 has two sliding installations inside the installation groove 601, the limiting block 604 has multiple fixed connections on the opposite surfaces of the two connecting blocks 603, the limiting groove 605 has multiple openings on the upper and lower sides of the moving block 602, and the limiting block 604 is located inside the limiting groove 605.

[0032] In the initial state, the limit block 604 is located in the limit groove 605, limiting the movement of the movable block 602, thereby fixing the position of the storage box 3 and ensuring the connection between the first channel 201 and the second channel 302, facilitating the migration of DNA molecules during the electrophoresis process; when the electrophoresis separation is completed, the limit block 604 is controlled to move out of the limit groove 605, and then the movable block 602 is pushed to drive the storage box 3 to move, so that the first channel 201 and the second channel 302 are misaligned and disconnected, preventing the small fragments of DNA separated into the storage cavity 301 from flowing back into the reaction hole 2, thereby ensuring the effectiveness of DNA separation and the accuracy of subsequent detection.

[0033] The limiting assembly 6 also includes a push block 606 and a spring 607 . The push block 606 is fixedly connected to one side of the two connecting blocks 603 . The spring 607 has two inner walls fixedly installed on the mounting groove 601 . The push block 606 is fixedly connected to one end of the spring 607 .

[0034] The spring 607 is always in a naturally extended state. In the initial state, the spring 607 applies a certain force to the push block 606, so that the push block 606 drives the connecting block 603 to move, and at the same time controls the limit block 604 to be stuck in the limit groove 605 to maintain the stability of the storage box 3; when the storage box 3 needs to be moved, the push block 606 is pushed to overcome the elastic force of the spring 607 and drive the connecting block 603 to move, so that the limit block 604 moves out of the limit groove 605, and then moves to the target position. The elastic force of the spring 607 will assist in fixing the moving block 602 in the new position to prevent it from moving at will, thereby playing a role of automatic positioning and fixing, simplifying the operation process, and improving the convenience and stability of the device.

[0035] A handle 7 is fixedly connected to the outer wall of the moving block 602 .

[0036] The handle 7 provides an operator with a convenient position for applying force. When the storage box 3 needs to be moved, the operator holds the handle 7 and cooperates with the limit assembly 6 to move the storage box 3, making the operation more convenient and accurate, and facilitating precise control of the position of the storage box 3, ensuring accurate docking or misalignment between the first channel 201 and the second channel 302.

[0037] Working principle: When in use, first fill the reaction well 2 and the storage chamber 301 with agarose gel of appropriate concentration, determine the agarose concentration according to experimental requirements to adjust the gel pore size, and then place the DNA sample inside the reaction well 2 and on top of the agarose gel.

[0038] Then, the power supply module 401 is started, and the annular negative electrode block 402 and the straight positive electrode block 403 are powered by the first power line 404 and the second power line 405 to form an electric field. At the same time, the power supply module 401 supplies power to the heating wire 501 through the third power line 502 to increase the temperature. Under the action of the electric field, the DNA molecules migrate to the storage cavity 301, small fragments of DNA enter the storage cavity 301, and large fragments of DNA remain in the reaction hole 2, thereby achieving DNA purification.

[0039] After the electrophoresis separation is completed, the push block 606 is pushed to overcome the elastic force of the spring 607 and drive the connecting block 603 to move, so that the limiting block 604 moves out of the limiting groove 605, and then the handle 7 is pulled to drive the moving block 602 to move, and the connecting block 603 slides in the installation groove 601, so that the first channel 201 and the second channel 302 are misaligned and disconnected, preventing the small fragment DNA from flowing back, and completing the DNA purification and separation process of the entire gene detection.

[0040] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A multi-well microplate device with high-throughput gene detection, comprising a plate body (1), characterized in that: The top of the plate body (1) is provided with a reaction hole (2), and the reaction holes (2) are arranged in a rectangular shape. A storage box (3) is slidably mounted inside the plate body (1), and the storage box (3) is arranged at the front end of each row of reaction holes (2). An electrophoresis mechanism (4) is mounted between the reaction hole (2) and the storage box (3), and a heating mechanism (5) is mounted at the bottom of the reaction hole (2). A limit position assembly (6) is mounted inside the plate body (1), and a first channel (201) is mounted at the front end of the reaction hole (2). A storage cavity (301) is mounted inside the storage box (3), and a second channel (302) is mounted at the front end of the storage box (3) and is in communication with the storage cavity (301). There are a plurality of second channels (302), and the first channel (201) and the second channel (302) are in communication with each other. The electrophoresis mechanism (4) comprises a power supply module (401), an annular negative electrode block (402) and a straight positive electrode block (403); the power supply module (401) is fixedly mounted inside the plate body (1); the annular negative electrode block (402) is fixedly mounted on the inner wall of the reaction hole (2); and the straight positive electrode block (403) is fixedly mounted on the inner wall of the storage cavity (301).

2. A multi-well microplate device with high-throughput gene detection according to claim 1, characterized in that: A first power line (404) is connected between the power supply module (401) and the annular negative electrode block (402), and a second power line (405) is connected between the power supply module (401) and the straight positive electrode block (403).

3. A multi-well microplate device with high-throughput gene detection according to claim 2, characterized in that: The temperature raising mechanism (5) comprises a heating wire (501) and a third power line (502); the heating wire (501) is fixedly mounted inside the plate body (1); the heating wire (501) is located at the bottom of each row of reaction holes (2); and the third power line (502) is connected between the power supply module (401) and the heating wire (501).

4. The multi-well microplate device with high-throughput gene detection according to claim 1, characterized in that: The limiting assembly (6) comprises a mounting groove (601), a moving block (602), a connecting block (603), a limiting block (604) and a limiting groove (605); the mounting groove (601) is arranged inside the plate body (1); the moving block (602) is fixedly connected to one side of a plurality of storage boxes (3); the moving block (602) is located inside the mounting groove (601); the connecting block (603) has two sliding parts installed inside the mounting groove (601); the limiting block (604) has a plurality of fixed parts connected to opposite surfaces of the two connecting blocks (603); the limiting groove (605) has a plurality of parts arranged on upper and lower sides of the moving block (602); and the limiting block (604) is located inside the limiting groove (605).

5. The multi-well microplate device with high-throughput gene detection according to claim 4, characterized in that: The limiting assembly (6) further comprises a push block (606) and a spring (607), wherein the push block (606) is fixedly connected to one side of the two connecting blocks (603), and the spring (607) has two inner walls fixedly mounted on the mounting groove (601), and the push block (606) is fixedly connected to one end of the spring (607).

6. The multi-well microplate device with high-throughput gene detection according to claim 4, characterized in that: A handle (7) is fixedly connected to the outer wall of the moving block (602).

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