Production process for improving pancreatin filtering performance

Abstract

The invention provides a production process for improving pancreatin filtering performance, which comprises the following steps: A) planing pig pancreas into fragments, grinding, adding protease, 25% alcoholic solution, calcium chloride and xylitol, and activating to obtain an activated material; b) performing slurry-residue separation on the activated material to obtain slurry; c) heating and activating the slurry to obtain an activated material; d) settling the activated material to obtain a light phase; then adding a solvent for precipitation, standing, removing supernate, and carrying out filter pressing to obtain a filter cake; and E) granulating the filter cake, degreasing with a mixed solution, collecting a heavy phase, and drying to obtain the pancreatin with good filtering performance. Low-temperature pulp-residue separation is creatively adopted, a large number of impurities can be prevented from entering pancreatin, and the filtering performance and purity of the pancreatin are improved. Acetone and ethyl alcohol are combined for degreasing, the fat removal rate is high, and the filtering performance is greatly improved.

Description

Technical Field

[0001] The invention relates to the field of biochemical pharmacy, and in particular to a production process for improving the filtration performance of pancreatic enzyme. Background Art

[0002] The pancreas is one of the scarce raw material resources in the world. According to the current research and development situation at home and abroad, there are 41 biochemical drugs extracted from the pancreas, including 6 hormones, 13 enzymes, 16 peptides, 1 amino acid preparation, 2 nucleic acid preparations, 1 polysaccharide, and 2 lipids. On the contrary, my country's abundant pig pancreas resources are not effectively utilized and developed. With the development of artificial synthetic insulin abroad, 95% of the global insulin market has been occupied by it, and the pig insulin market is shrinking, resulting in greater waste of the pancreas. The pancreatic enzymes currently provided by the market are mainly trypsin, lipase, and amylase, and the market price is relatively low; the pancreatic enzymes used for the purification of pancreatic kallikreinogenase are rarely developed, but pancreatic kallikreinogenase has broad market prospects, higher returns and profits, and strong market potential.

[0003] Pancreatic kallikrein, also known as pancreatic kallikrein and vasodilator, is widely present in the pancreas, submandibular gland and saliva of the human body, with the highest content in the pancreas. In the prior art, pancreatic kallikrein is a proteolytic enzyme extracted from the pig pancreas, composed of 18 amino acids and 4 sugars, and is an endoproteolytic enzyme with a molecular weight of 26,800. In the body, the enzyme exists in the form of a zymogen, i.e., a kallikreinogen. The human body has a wide range of physiological effects on kallikrein, and its pharmaceutical preparations can be used to treat hypertension, cardiovascular and cerebrovascular diseases, diabetic nephropathy, male infertility, in addition to this, it can also be used to treat abnormal contraction of vascular smooth muscle, angina pectoris, vasospasm, limb paresthesia, etc., and it is also widely used in the emerging health care products and anti-aging cosmetics industries.

[0004] As a mixture containing as many as 15 enzymes, pancreatic enzyme faces a major problem in subsequent deep processing: its poor filtration performance and long filtration time seriously affect the production progress; moreover, when the temperature exceeds 30°C, the inactivation rate of various enzymes in pancreatic enzyme accelerates, greatly reducing its economic value.

[0005] Therefore, it is very necessary to develop a pancreatic enzyme with excellent filtration performance to provide raw materials for downstream companies to purify single enzymes. Summary of the invention

[0006] In view of this, the technical problem to be solved by the present invention is to provide a pancreatic enzyme with good filtration performance. The pancreatic enzyme provided by the present invention has good filtration performance.

[0007] The present invention provides a production process for improving the filtration performance of pancreatic enzyme, comprising the following steps:

[0008] A) Shaving the pig pancreas into pieces, grinding it, adding protease, 25% alcohol solution, calcium chloride, and xylitol to activate it, and obtaining an activated material;

[0009] B) separating the activated material into pulp and residue to obtain slurry;

[0010] C) heating the slurry to activate it, to obtain an activated material;

[0011] D) sedimenting the activated material to obtain a light phase; then adding a solvent for precipitation, standing, removing the supernatant, and filtering to obtain a filter cake;

[0012] E) The filter cake is granulated and then defatted with a mixed solution, and the heavy phase is collected and dried to obtain pancreatic enzyme.

[0013] The production process for improving the filtration performance of pancreatic enzyme provided by the present invention firstly shaves the pig pancreas into pieces. The present invention preferably uses a flaker to shave the pieces, which is well known to those skilled in the art and is not limited by the present invention.

[0014] Then, grinding is performed, preferably using a colloid mill, which is well known to those skilled in the art and is not limited in the present invention.

[0015] After grinding, the mixture is put into a processing tank, and papain, 25% alcohol solution, calcium chloride and xylitol are added while stirring to activate the mixture, thereby obtaining an activated material.

[0016] According to the present invention, the mass ratio of the protease, 25% alcohol solution, calcium chloride, xylitol and pig pancreas is (1-2):(7-9):(0.5-1.0):(0.5-0.7):100.

[0017] In some embodiments, the mass ratio of the protease, 25% alcohol solution, calcium chloride, xylitol and porcine pancreas is (1-2):(7-8):(0.6-0.8):(0.5-0.65):100.

[0018] In some embodiments, the mass ratio of the protease, 25% alcohol solution, calcium chloride, xylitol and porcine pancreas is (1.5-1.8):(7.5-8):(0.6-0.8):(0.6-0.65):100.

[0019] In some embodiments, the mass ratio of the protease, lactic acid, calcium chloride, xylitol and porcine pancreas is 1.5:8:0.7:0.6:100;

[0020] In some embodiments, the mass ratio of the protease, lactic acid, calcium chloride, xylitol and porcine pancreas is 1.6:7.5:0.8:0.6:100.

[0021] In some embodiments, the mass ratio of the protease, lactic acid, calcium chloride, xylitol and porcine pancreas is 1.8:8.0:0.7:0.65:100.

[0022] The activation parameters of the present invention are 8-12°C, 5h, and can be specifically 8°C, 9°C, 10°C, 11°C or 12°C, or a range between any two of the above.

[0023] The activated material is passed through a pulp-residue separator at 10-13°C to remove fibers, and fiber tissue and pulp are obtained. The pulp should be free of fibers, and then the pulp is pumped into an extraction tank. Specifically, the pulp-residue separation temperature is 10°C, 11°C, 12°C or 13°C; or a range between any two of the above.

[0024] The present invention creatively adopts low-temperature pulp-residue separation to prevent a large amount of impurities from entering the pancreatic enzyme, thereby improving the filtration performance and purity of the pancreatic enzyme.

[0025] The slurry is heated and activated to obtain an activated material.

[0026] The activation parameters of the present invention include: slowly raising the temperature to 21-23° C. over 8-10 hours, and keeping the temperature for 8-10 hours.

[0027] The activated material is allowed to settle to obtain a light phase, and then a solvent is added for precipitation and allowed to stand. The preferred method specifically includes: after the activation is completed, stirring of the material is stopped and the material is allowed to settle for 1-2 hours.

[0028] Add isopropanol (15°C) to the settled light phase while stirring, adjust the alcohol content to 55-58°, and let it stand for 3 to 5 hours.

[0029] The supernatant is removed, filtered and filtered to obtain a filter cake. The preferred method of removing the supernatant in the present invention is to siphon the supernatant, and then pack the heavy phase into bags (45-50 kg / bag) and filter it centrally.

[0030] The filter cake is granulated and then defatted with a mixed solution.

[0031] According to the present invention, the degreasing mixed solution includes acetone and ethanol; the mass ratio of the filter cake, acetone and ethanol is 1:(1-2):(1-2); more preferably 1:1.5:1.5. The immersed material shall prevail.

[0032] The degreasing temperature of the present invention is 45-48°C; specifically, it can be 45°C, 46°C, 47°C, 48°C or a range between any two of the above.

[0033] The present invention adopts acetone and ethanol combined degreasing, the fat removal rate is high, and the filtering performance is greatly improved; at the same time, the degreasing temperature is 45-48°C, and the effect is best.

[0034] The heavy phase was collected and dried to obtain crude pancreatic kallikreinogenase.

[0035] In one embodiment of the present invention, the material to be dried is transferred into the barrel of the dryer, sealed, and the air inlet is turned on, the air inlet temperature is set to 80-85°C, the boiling dryer is turned on, the mixing temperature is 65-68°C, and the drying time is about 4-6 hours to obtain pancreatin.

[0036] The moisture content is controlled at ≤6% and the fineness is controlled at 20-40 mesh.

[0037] The present invention provides a pancreatic enzyme with high filtration performance, which is prepared by the preparation method described in any one of the above technical solutions.

[0038] The present invention provides a pancreatic kallikreinogenase product, which is prepared by purifying the pancreatic kallikreinogenase described in the above technical solution.

[0039] The present invention provides a production process for improving the filtration performance of pancreatic enzyme, comprising the following steps: A) shaving the pig pancreas into pieces, grinding, adding protease, lactic acid, calcium chloride, and xylitol for activation to obtain an activated material; B) performing pulp-residue separation on the activated material to obtain a slurry; C) heating the slurry for activation to obtain an activated material; D) settling the activated material to obtain a light phase; then adding a solvent for precipitation, standing, removing the supernatant, and filtering to obtain a filter cake; E) granulating the filter cake and defatting it with a mixed solution, collecting the heavy phase and drying it to obtain a crude pancreatic kallikreinogenase. The present invention creatively uses low-temperature pulp-residue separation to avoid a large amount of impurities from entering the pancreatic enzyme, thereby improving the filtration performance and purity of the pancreatic enzyme. The present invention uses a combination of acetone and ethanol for degreasing, with a high fat removal rate, which greatly improves the filtration performance. DETAILED DESCRIPTION

[0040] The present invention provides a production process for improving the filtration performance of pancreatic enzymes. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It is particularly important to point out that all similar replacements and modifications are obvious to those skilled in the art, and they all belong to the scope of protection of the present invention. The methods and applications of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications of this article without departing from the content, spirit and scope of the present invention to realize and apply the technology of the present invention.

[0041] It should be understood that the expression "one or more of..." includes each of the items recited after the expression individually and in various different combinations of two or more of the recited items, unless otherwise understood from the context and usage. The expression "and / or" in combination with three or more recited items should be understood to have the same meaning, unless otherwise understood from the context.

[0042] The use of the terms "comprising", "having" or "containing", including their grammatical synonyms, should generally be understood as open and non-restrictive, for example not excluding other unrecited elements or steps, unless otherwise specifically stated or otherwise understood from the context.

[0043] In this application, the term "and / or" describes the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. A and B can be singular or plural.

[0044] In this application, "at least one" means one or more, "more than one" means two or more. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single or plural items.

[0045] It should be understood that the order of steps or the order in which certain actions are performed is not important as long as the present invention remains operable. In addition, two or more steps or actions may be performed simultaneously.

[0046] The use of any and all examples or exemplary language, such as "for example" or "including", herein is intended only to better illustrate the invention and does not limit the scope of the invention unless otherwise claimed. No language in this specification should be construed as indicating that any non-claimed element is essential to the practice of the invention.

[0047] In addition, the numerical ranges and parameters used to define the present invention are approximate values, and the relevant values ​​in the specific embodiments have been presented as accurately as possible. However, any numerical value inherently inevitably contains standard deviations due to individual test methods. Therefore, unless otherwise expressly stated, it should be understood that all ranges, quantities, values ​​and percentages used in this disclosure are modified by "about". Here, "about" generally means that the actual value is within plus or minus 10%, 5%, 1% or 0.5% of a specific value or range.

[0048] It should be understood that in the various embodiments of the present application, the size of the serial numbers of the above-mentioned processes does not mean the order of execution, some or all of the steps can be executed in parallel or sequentially, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present application.

[0049] Some cases are recorded in the embodiments and comparative examples of the present invention, wherein the embodiments show certain implementation modes of the present invention. However, this does not mean that the effects of the present invention can only be achieved in these cases.

[0050] In order to further illustrate the present invention, a production process for improving the filtration performance of pancreatic enzyme provided by the present invention is described in detail below in conjunction with examples.

[0051] Example 1

[0052] (1) Activation

[0053] The pig pancreas was shaved into pieces (flaker), ground (colloid mill) and then put into the pretreatment tank. Papain, 25% alcohol solution, calcium chloride and xylitol were added while stirring at a speed of 30HZ. The concentrations of papain, 25% alcohol solution, calcium chloride and xylitol (in proportion to the mass of the pancreas) were 1.5%, 8%, 0.7% and 0.6% respectively. The temperature was slowly raised for 5 hours until the material temperature reached 12°C and activation began.

[0054] (2) Pulp and Slag Separation

[0055] The activated material is passed through a pulp separator at 12°C to remove fibers, and fiber tissue and slurry are obtained. The slurry should be free of fibers, and then the slurry is pumped into an extraction tank.

[0056] (3) Activation

[0057] The temperature of the material was slowly raised to 23°C over 10 hours and the material was kept at this temperature for 8 hours.

[0058] (4) Settlement

[0059] After activation, the material was stopped from being stirred and allowed to settle for 2 hours.

[0060] (5) Sedimentation

[0061] Add isopropanol (15°C) to the settled light phase under stirring, adjust the alcohol content to 55-58°, and let it stand for 3 hours.

[0062] (6) Filter pressing.

[0063] Siphon the supernatant, bag the heavy phase (45-50kg / bag) and filter press it centrally.

[0064] (7) Degreasing

[0065] After the filter cake is granulated, it is put into a degreasing tank and degreased with appropriate amounts of acetone and ethanol (material: acetone: ethanol is 1:1.5:1.5) (based on the immersed material) at a degreasing temperature of 45°C. The light phase is pumped out and the heavy phase is collected for drying.

[0066] (8) Drying.

[0067] Transfer the material to be dried into the barrel of the dryer, seal it, open the air inlet, set the air inlet temperature to 80-85℃, start boiling drying, and the mixing temperature is preferably 65-68℃. The drying time is about 6 hours. Get pancreatin. The moisture content is controlled to ≤6%, and the fineness is controlled to 20-40 mesh.

[0068] Example 2

[0069] (1) Activation

[0070] The pig pancreas was shaved into pieces (flaker), ground (colloid mill) and then put into the pretreatment tank. Papain, 25% alcohol solution, calcium chloride and xylitol were added while stirring at a speed of 30HZ. The concentrations of papain, 25% alcohol solution, calcium chloride and xylitol (in proportion to the mass of the pancreas) were 1.6%, 7.5%, 0.8% and 0.6% respectively. The temperature was slowly raised for 5 hours until the material temperature reached 10°C and activation began.

[0071] (2) Pulp and Slag Separation

[0072] The activated material is passed through a pulp separator at 10°C to remove fibers, and fiber tissue and slurry are obtained. The slurry should be free of fibers, and then the slurry is pumped into an extraction tank.

[0073] (3) Activation

[0074] The temperature of the material was slowly raised to 23°C over 10 hours and the material was kept at this temperature for 8 hours.

[0075] (4) Settlement

[0076] After activation, the material was stopped from being stirred and allowed to settle for 2 hours.

[0077] (5) Sedimentation

[0078] Add isopropanol (15°C) to the settled light phase under stirring, adjust the alcohol content to 55-58°, and let it stand for 3 hours.

[0079] (6) Filter pressing.

[0080] Siphon the supernatant, bag the heavy phase (45-50kg / bag) and filter press it centrally.

[0081] (7) Degreasing

[0082] After the filter cake is granulated, it is put into a degreasing tank and degreased with appropriate amounts of acetone and ethanol (material: acetone: ethanol is 1:1.5:1.5) (based on the immersed material) at a degreasing temperature of 47°C. The light phase is pumped out and the heavy phase is collected for drying.

[0083] (8) Drying.

[0084] Transfer the material to be dried into the barrel of the dryer, seal it, open the air inlet, set the air inlet temperature to 80-85℃, start boiling drying, and the mixing temperature is preferably 65-68℃. The drying time is about 5 hours. Pancreatin is obtained. The moisture content is controlled to ≤6%, and the fineness is controlled to 20-40 mesh.

[0085] Example 3

[0086] (1) Activation

[0087] The pig pancreas was shaved into pieces (flaker), ground (colloid mill) and then put into the pretreatment tank. Papain, 25% alcohol solution, calcium chloride and xylitol were added while stirring at a speed of 30HZ. The concentrations of papain, 25% alcohol solution, calcium chloride and xylitol (in proportion to the mass of the pancreas) were 1.8%, 8.2%, 0.7% and 0.65% respectively. The temperature was slowly raised for 5 hours until the material temperature reached 13°C and activation began.

[0088] (2) Pulp and Slag Separation

[0089] The activated material is passed through a pulp separator at 13°C to remove fibers, and fiber tissue and slurry are obtained. The slurry should be free of fibers, and then the slurry is pumped into an extraction tank.

[0090] (3) Activation

[0091] The temperature of the material was slowly raised to 23°C over 8 hours and the material was kept at this temperature for 8 hours.

[0092] (4) Settlement

[0093] After activation, the material was stopped from being stirred and allowed to settle for 2 hours.

[0094] (5) Sedimentation

[0095] Add isopropanol (15°C) to the settled light phase under stirring, adjust the alcohol content to 55-58°, and let it stand for 3 hours.

[0096] (6) Filter pressing.

[0097] Siphon the supernatant, bag the heavy phase (45-50kg / bag) and filter press it centrally.

[0098] (7) Degreasing

[0099] After the filter cake is granulated, it is put into a degreasing tank and degreased with appropriate amounts of acetone and ethanol (material: acetone: ethanol is 1:1.5:1.5) (based on the immersed material) at a degreasing temperature of 48°C. The light phase is removed and the heavy phase is collected for drying.

[0100] (8) Drying.

[0101] Transfer the material to be dried into the barrel of the dryer, seal it, open the air inlet, set the air inlet temperature to 80-85℃, start boiling drying, and the mixing temperature is preferably 65-68℃. The drying time is about 4-6 hours. Get pancreatin. The moisture content is controlled to ≤6%, and the fineness is controlled to 20-40 mesh.

[0102] Comparative Example 1

[0103] Activation: After shaving and grinding the pig pancreas, put it into the pretreatment tank, add papain, 25% alcohol solution, calcium chloride, and xylitol in proportions of 1.8%, 8.2%, 0.7%, and 0.65% (together with the mass of the pancreas), heat it for 13-15 hours, raise the material temperature to 30°C, and start keeping warm for 3 hours; pulp-residue separation: the material is passed through a pulp-residue separator to remove fibers; sedimentation: after activation, stop stirring the material and let it stand for 2 hours; precipitation: add isopropanol (15°C) to the light phase under stirring, adjust the alcohol content to 55-58°, and let it stand for 5 hours; filter pressing: siphon the supernatant, bag the heavy phase (45-50kg / bag), and then filter press it centrally; after granulating the defatted filter cake, put it into a defatting tank, add appropriate amounts of acetone and ethanol (material: acetone: ethanol is 1:1.5:1.5) (based on the immersed material) for defatting, and the defatting temperature is 20-22°C. Remove the light phase and collect the heavy phase; Drying: transfer the material to be dried into the barrel of the dryer, seal it, open the air inlet, set the air inlet temperature to 80-85℃, open the boiling dryer, and the mixing temperature is preferably 65-68℃. The drying time is about 4-6 hours. Get pancreatin. The moisture content is controlled to ≤6%, and the fineness is controlled to 20-40 mesh.

[0104] Comparative Example 2

[0105] Activation: After shaving and grinding the pig pancreas, put it into the pretreatment tank, add papain, 25% alcohol solution, calcium chloride, and xylitol in proportions of 1.8%, 8.2%, 0.7%, and 0.65% (together with the mass of the pancreas), raise the temperature for 13-15 hours, raise the material temperature to 25°C and start keeping warm for 3 hours; pulp-residue separation: the material is passed through a pulp-residue separator to remove fibers; sedimentation: after activation, stop stirring the material and let it settle for 2 hours; precipitation: add isopropanol (15°C) to the light phase under stirring, and adjust the alcohol content to 55- 58°, let stand for 5 hours; filter press: siphon the supernatant, bag the heavy phase (45-50kg / bag) and then filter press; put the defatted filter cake into the defatting tank after granulation, add appropriate amount of acetone and ethanol (material: acetone: ethanol is 1:1.5:1.5) (based on the immersed material) for defatting, the defatting temperature is 23-24℃) to remove the light phase and collect the heavy phase; drying: transfer the material to be dried to the material barrel of the dryer, seal it well, turn on the air inlet, set the air inlet temperature to 80-85℃, turn on the boiling dryer, and the mixing temperature is preferably 65-68℃. The drying time is about 4-6 hours. Get pancreatin. The moisture content is controlled to ≤6%, and the fineness is controlled to 20-40 mesh.

[0106] Comparative Example 3

[0107] Activation: After shaving and grinding the pig pancreas, put it into the pretreatment tank, add papain, 25% alcohol solution, calcium chloride, and xylitol in proportions of 1.8%, 8.2%, 0.7%, and 0.60% (together with the mass of the pancreas), heat it for 14-15 hours, raise the material temperature to 30°C, and start keeping warm for 3 hours; pulp-residue separation: the material is passed through a pulp-residue separator to remove fibers; sedimentation: after activation, stop stirring the material and let it stand for 2 hours; precipitation: add isopropanol (15°C) to the light phase under stirring, adjust the alcohol content to 56-58°, and let it stand for 5 hours; filter pressing: siphon the supernatant, bag the heavy phase (45-50kg / bag), and then filter press it centrally; after granulating the defatted filter cake, put it into the defatting tank, add appropriate amounts of acetone and ethanol (material: acetone: ethanol is 1:1.5:1.5) (based on the immersed material) for defatting, and the defatting temperature is 20-25°C. Remove the light phase and collect the heavy phase; Drying: transfer the material to be dried into the barrel of the dryer, seal it, open the air inlet, set the air inlet temperature to 80-85℃, open the boiling dryer, and the mixing temperature is preferably 65-68℃. The drying time is about 4-6 hours. Get pancreatin. The moisture content is controlled to ≤6%, and the fineness is controlled to 20-40 mesh.

[0108] Comparison of the effects of low temperature separation (de-slurrying) and elevated temperature degreasing

[0109]

[0110] As can be seen from the above table, other fibrin impurities, connective tissue, etc. are not decomposed during separation at low temperature (10-13°C), and are removed in advance after separation, without affecting or interfering with the subsequent pancreatic enzyme extraction. At the same time, due to the removal of impurity proteins, the activity of pancreatic enzymes is improved. At this time, appropriate (10%-20% filter aid) can be added to improve the filterability of pancreatic enzymes to a certain extent. When other manufacturers remove pulp at high temperature (around 30°C), other impurity proteins enter the pancreatic enzymes, affecting their filterability.

[0111] Verification Example

[0112] 1.1 Pancreatic enzyme filtration performance test

[0113] 1. Accurately weigh 200.0g of the test pancreatic enzyme sample and slowly dissolve it into a 5-liter stainless steel pot while stirring (5000g of drinking water below 20°C with no corrupt odor and no foreign matter has been added to the pot in advance at a ratio of 1:25).

[0114] 2. Use a dropper to add 8% oxalic acid solution to adjust the pH of the pancreatic enzyme solution to 4.0-4.2. Stir for 5-10 minutes and re-measure the pH, which should be 4.0-4.2. If it is not within this range, continue to add oxalic acid to dissolve and adjust. When the pH value stabilizes at 4.0-4.2, continue to stir and extract for 0.5-1 hour.

[0115] 3. After the extraction, add 40-50g of perlite and filter it through a funnel with a diameter of 30cm and a height of 13.5cm and pure cotton filter cloth (the filtering time should be within 3 hours). During the static filtering period, the filter cloth and materials are not allowed to be turned over.

[0116] Comparative Example 4 of the present invention is the same as Comparative Example 2, except that the material temperature is raised to 24° C. and then kept warm for 5 hours.

[0117] Comparative Example 5 is the same as Comparative Example 1, except that the degreasing temperature is 32-34°C.

[0118] Comparative Example 6 is the same as Comparative Example 1, except that the degreasing temperature is 36-37°C.

[0119] Example 4 is the same as Example 1, except that the desizing temperature is 13°C. Example 5 is the same as Example 1, except that the degreasing temperature is 48°C.

[0120] Example 6 is the same as Example 1, except that the desizing temperature is 12°C, the degreasing temperature is 46°C,

[0121]

[0122]

[0123] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A production process for improving the filtration performance of pancreatic enzyme, characterized in that: The steps include: A) Shaving the pig pancreas into pieces, grinding it, adding protease, 25% alcohol solution, calcium chloride, and xylitol to activate it, and obtaining an activated material; B) separating the activated material into pulp and residue to obtain slurry; C) heating the slurry to activate it, to obtain an activated material; D) sedimenting the activated material to obtain a light phase; then adding a solvent for precipitation, standing, removing the supernatant, and filtering to obtain a filter cake; E) The filter cake is granulated and then defatted with a mixed solution, and the heavy phase is collected and dried to obtain pancreatic enzyme.

2. The production process according to claim 1, characterized in that: Step A) The mass ratio of the protease, 25% alcohol solution, calcium chloride, xylitol and porcine pancreas is (1-2):(7-9):(0.5-1.0):(0.5-0.7):

100.

3. The production process according to claim 1, characterized in that: The activation parameters of step A) are 8-12° C., 5 h.

4. The production process according to claim 1, characterized in that: The temperature of the pulp-residue separation in step B) is 10-13°C.

5. The production process according to claim 1, characterized in that: Step C) The activation parameters include: slowly raising the temperature to 21-23° C. over 8-10 hours and keeping the temperature for 8-10 hours.

6. The production process according to claim 1, characterized in that: Step D) the sedimentation time is 1 to 2 hours; The solvent for precipitation is isopropanol, and then the alcohol content is adjusted to 55-58° and left to stand for 3-5 hours.

7. The production process according to claim 1, characterized in that: Step E) the degreasing mixed solution comprises acetone and ethanol; the mass ratio of the filter cake, acetone and ethanol is 1:(1-2):(1-2); the degreasing temperature is 45-48°C.

8. The production process according to claim 1, characterized in that: Step E) The drying parameters include: 65-68° C., 4-6 hours.

9. A pancreatic enzyme with excellent filtration performance, characterized in that: The compound is prepared by the preparation method according to any one of claims 1 to 8.

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