Magnetic separation module and high-speed fully-automatic chemiluminescence immunoassay analyzer

Abstract

The application belongs to the technical field of immunochemical analysis and detection technology, and particularly relates to a magnetic separation module and a high-speed full-automatic chemiluminescence immunoassay analyzer. The magnetic separation module comprises a magnetic separation bottom plate assembly, a magnetic separation pot assembly and a magnetic separation liquid suction needle assembly are arranged on the upper top surface of the magnetic separation bottom plate assembly, and a magnetic separation mixing assembly is arranged below the magnetic separation bottom plate assembly. The high-speed full-automatic chemiluminescence immunoassay analyzer comprises the magnetic separation module.

Description

Technical Field

[0001] The present invention belongs to the technical field of immunochemical analysis and detection technology, and particularly relates to a magnetic separation module and a high-speed fully automatic chemiluminescence immunoassay analyzer. Background Art

[0002] A chemiluminescent immunoassay analyzer is an analytical instrument that combines chemiluminescence analysis with immunoassays. It utilizes the specific binding reaction between antigens and antibodies, combined with chemiluminescence technology, to detect the content of specific substances in a sample. For example, when testing for thyroid hormone in the blood, the thyroid hormone (antigen) specifically binds to the corresponding thyroid hormone antibody in the instrument. Its immunological principle is to utilize the specific binding properties of antigens and antibodies. The sample to be tested reacts with an antibody (or antigen) labeled with a chemiluminescent substance to form an antigen-antibody complex.

[0003] The steps for analysis and detection using a chemiluminescence immunoassay are as follows: first, the sample to be tested is pretreated. For example, biological samples such as blood and urine may require centrifugation and dilution. The treated sample is then immunoreacted with an antibody labeled with a chemiluminescent substance to form an antigen-antibody complex. Unbound substances are then removed through washing to reduce nonspecific background signals. The substrate that produces the chemiluminescent reaction is then added to the reaction system, and the substrate reacts with the labeled enzyme to produce a chemiluminescent signal. Finally, an optical detector such as a photomultiplier tube or photodiode is used to convert the chemiluminescent signal into an electrical signal for detection and analysis, thereby obtaining information such as the concentration of the target substance in the sample. Due to the significant technical advantages of high sensitivity, high specificity, wide linear range, and rapid detection, chemiluminescence immunoassays are widely used in clinical diagnosis and scientific research.

[0004] According to the structure, the chemiluminescence immunoassay instrument includes a sample processing system, a reagent storage and distribution system, a reaction system, a detection system, and a computer control system. The reaction system includes the reagent disk module, sample needle module, magnetic separation module and other structures mentioned in this solution. The magnetic separation modules currently prevalent on the market usually adopt a structure in which the cleaning fluid and the substrate are mixed simultaneously, which makes it difficult to meet the mixing strength requirements of the cleaning fluid and the substrate at the same time. The defects of the simultaneous mixing of the cleaning fluid and the substrate are relatively obvious, mainly including: 1. Risk of cross-contamination; 2. Reduced reaction stability; 3. Unsatisfactory mixing effect; 4. Increased difficulty in instrument maintenance.

[0005] In order to isolate and mix the cleaning solution and substrate with different intensities, the present invention hopes to provide a magnetic separation module and a high-speed fully automatic chemiluminescence immunoassay analyzer. Summary of the Invention

[0006] The object of the present invention is to provide a magnetic separation module and a high-speed fully automatic chemiluminescence immunoassay analyzer to solve the problems raised in the above background technology.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A magnetic separation module comprises a magnetic separation base plate assembly, wherein a magnetic separation pot assembly and a magnetic separation pipette needle assembly are provided on the upper surface of the magnetic separation base plate assembly, and a magnetic separation mixing assembly is provided below the magnetic separation base plate assembly;

[0009] The magnetic separation pot assembly includes a magnetic separation base, a magnetic separation disk cover, a rotating disk, a rotating disk bearing, a main drive pulley and a main drive motor; the magnetic separation base is fixed to the upper surface of the magnetic separation bottom plate assembly, and a rotating disk bearing is provided on the magnetic separation base, the upper end of the rotating disk bearing is fixedly connected to the rotating disk, the lower end of the rotating disk bearing is fixedly connected to the main drive pulley, the main drive motor is fixed to the lower surface of the magnetic separation bottom plate assembly, and the main drive motor and the main drive pulley are in driving connection; the rotating disk is provided with a number of reaction cup placement slots evenly distributed in a circular array, and the reaction cup placement slots are provided on the rotating disk. A reaction cup is provided in the groove; the magnetic separation disk cover is provided with a cup changing port, a cleaning liquid injection needle, a plurality of cleaning liquid mixing and pressing assemblies and a substrate mixing and pressing assemblies arranged in a circular array in sequence; the cleaning liquid mixing and pressing assemblies include a cleaning liquid aspiration needle assembly and a cleaning liquid injection needle assembly, and the substrate mixing and pressing assemblies include a substrate aspiration needle assembly and a substrate mixing and pressing assemblies; as the rotating disk rotates through several stations, the cup changing port, the cleaning liquid injection needle, the cleaning liquid aspiration needle assembly, the cleaning liquid injection needle assembly, the substrate aspiration needle assembly and the substrate mixing and pressing assemblies are always located directly above one of the reaction cup placement grooves;

[0010] The magnetic separation pipetting needle assembly includes a lifter 1 and a pipetting needle fixing plate, a lifter 1 is provided between the magnetic separation bottom plate assembly and the pipetting needle fixing plate, and the pipetting needle fixing plate is located directly above the magnetic separation pot assembly; the cleaning liquid pipetting needle assembly includes a cleaning liquid pipetting needle, and the substrate pipetting needle assembly includes a substrate pipetting needle, and the upper half of the cleaning liquid pipetting needle and the upper half of the substrate pipetting needle are both fixed to the pipetting needle fixing plate;

[0011] The magnetic separation mixing assembly includes a C-shaped fixed plate, a plurality of cleaning liquid mixing columns, a substrate mixing column, a second elevator, a cleaning liquid mixing motor, and a substrate mixing motor; the plurality of cleaning liquid mixing columns and the substrate mixing columns are arranged in sequence on the C-shaped fixed plate, the plurality of cleaning liquid mixing columns and the plurality of cleaning liquid injection needle assemblies correspond one to one and are located on the same vertical axis, and the substrate mixing column and the substrate mixing gland are located on the same vertical axis; a cleaning liquid mixing synchronous belt is provided between the cleaning liquid mixing motor and the plurality of cleaning liquid mixing columns, and a substrate mixing synchronous belt is provided between the substrate mixing motor and the substrate mixing column; a second elevator is provided between the C-shaped fixed plate and the magnetic separation bottom plate assembly;

[0012] The cleaning liquid mixing column is rotatably connected to the C-shaped fixed plate, and a reaction cup fixing groove is provided on the top of the cleaning liquid mixing column; a cleaning liquid mixing driven wheel is provided at the bottom of the cleaning liquid mixing column, and a cleaning liquid mixing synchronous belt is provided between the cleaning liquid mixing motor and the plurality of cleaning liquid mixing driven wheels; a plurality of cleaning liquid mixing tensioning wheels are provided on the lower bottom surface of the C-shaped fixed plate, and the plurality of cleaning liquid mixing tensioning wheels are connected to the cleaning liquid mixing synchronous belt;

[0013] The substrate mixing column is rotatably connected to the C-shaped fixed plate, and a reaction cup fixing groove is provided on the top of the substrate mixing column; a substrate mixing driven wheel is provided at the bottom of the substrate mixing column, and a substrate mixing synchronous belt is provided between the substrate mixing motor and the plurality of substrate mixing driven wheels; a plurality of substrate mixing tensioning wheels are provided on the lower bottom surface of the C-shaped fixed plate, and the plurality of substrate mixing tensioning wheels are connected to the substrate mixing synchronous belt.

[0014] Preferably, the cleaning liquid aspiration needle assembly includes a cleaning liquid aspiration needle, the upper half of which is fixed to the aspiration needle fixing plate, and the lower half of which can be inserted into the interior of the magnetic separation pot assembly; as the rotating disk rotates through several positions, the lower end of the cleaning liquid aspiration needle is always aligned with one of the reaction cup placement slots.

[0015] Preferably, the cleaning liquid injection needle assembly includes a cleaning liquid injection needle, a mounting seat, a bearing, a rotating hollow shaft, a return spring and a pressure capping head; a bearing is provided at the mounting seat, the lower end of the bearing is connected to the rotating hollow shaft, the lower end of the rotating hollow shaft is nested with the pressure capping head, a return spring is provided inside the rotating hollow shaft, and the two ends of the return spring respectively abut the inner wall of the rotating hollow shaft and the top of the pressure capping head; the cleaning liquid injection needle passes through the bearing and the rotating hollow shaft in sequence, and the end of the cleaning liquid injection needle is connected to the pressure capping head; a guide pin is provided on the outer wall of the pressure capping head, and correspondingly, a guide groove is provided on the side wall of the rotating hollow shaft; as the rotating disk rotates through several stations, the lower end of the pressure capping head is always aligned with one of the reaction cup placement slots.

[0016] Preferably, the substrate pipetting needle assembly includes a substrate pipetting needle and a stabilizing seat, the upper half of the substrate pipetting needle is fixed to the pipetting needle fixing plate, and the lower half of the substrate pipetting needle passes through the stabilizing seat; as the rotating disk rotates through several stations, the lower end of the substrate pipetting needle is always aligned with one of the reaction cup placement slots.

[0017] Preferably, the substrate mixing cover includes a bearing, a cover head and a return spring, the bearing is installed on the magnetic separation disc cover, the cover head passes through the bearing, and a return spring is provided between the bearing and the cover head; a guide pin is provided at the bearing, and correspondingly, a guide groove is provided at the cover head.

[0018] Preferably, the elevator includes a fixed rail, a sliding rail and a lifting motor; the fixed rail is fixedly arranged on the magnetic separation base plate assembly, the lifting motor is fixedly arranged below the fixed rail, the sliding rail is in a limited fit with the fixed rail, and the sliding rail can slide up and down along the fixed rail; the upper end of the sliding rail is fixedly connected to the pipette needle fixing plate, and the lower end of the sliding rail is provided with a lifting worm, and the lifting worm is connected to the lifting motor.

[0019] Preferably, elevator 2 includes fixed rail 2, sliding rail 2 and lifting motor 2; the fixed rail 2 is fixedly arranged on the magnetic separation base plate assembly, the sliding rail 2 is fixedly arranged on the C-shaped fixed plate, the sliding rail 2 and the fixed rail 2 are in a limited fit, and the sliding rail 2 can slide along the fixed rail 2; the lifting motor 2 is fixedly arranged at a position below the fixed rail 2, and a lifting worm 2 is provided on the sliding rail 2, and the lifting worm 2 is connected to the lifting motor 2.

[0020] A high-speed fully automatic chemiluminescence immunoassay analyzer comprises a magnetic separation module.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] (1) The present invention provides a magnetic separation module, wherein the magnetic separation mixing component includes three cleaning liquid mixing columns and one substrate mixing column. The three cleaning liquid mixing columns are connected to the same cleaning liquid mixing motor, and the substrate mixing column is connected to the substrate mixing motor. At the same time, a rotating disk structure is adopted. The reaction cup can complete the mixing operation of the cleaning liquid and the substrate respectively during the rotation of the rotating disk. The cleaning liquid and the substrate can be mixed separately to achieve different mixing intensity requirements;

[0023] (2) The magnetic separation module provided by the present invention adopts a square reaction cup structure. When the square reaction cup rotates under the drive of the mixing drive column, the square reaction cup can achieve self-rotation mixing, and the mixing consistency effect is good;

[0024] (3) The present invention provides a magnetic separation module, which uses a magnetic separation mixing component to move upward, and after the corresponding reaction cup is lifted up, the corresponding pressure cover head just covers the reaction cup, and then the liquid injection and self-rotation mixing begin; after the self-rotation mixing, the reaction cup is transferred to the next cleaning liquid aspiration needle component or substrate aspiration needle component for aspiration. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the structure of a high-speed fully automatic chemiluminescence immunoassay analyzer;

[0026] Figure 2 This is a schematic diagram of the disassembled structure of a high-speed fully automatic chemiluminescence immunoassay analyzer;

[0027] Figure 3 It is a structural diagram of the magnetic separation module;

[0028] Figure 4 Schematic diagram of the disassembled structure of the magnetic separation module Figure 1 ;

[0029] Figure 5 Schematic diagram of the disassembled structure of the magnetic separation module Figure 2 ;

[0030] Figure 6 This is a side and lower view of the magnetic separation module;

[0031] Figure 7 Schematic diagram of the structure of the magnetic separation and mixing component Figure 1 ;

[0032] Figure 8 Schematic diagram of the structure of the magnetic separation disk cover;

[0033] Figure 9 Schematic diagram of the structure of the magnetic separation and mixing component Figure 2 ;

[0034] Figure 10 Schematic diagram of the structure of the reaction cup, cleaning solution mixing column / substrate mixing column;

[0035] Figure 11 is a cross-sectional view of the cleaning fluid injection needle assembly;

[0036] Figure 12 It is a cross-sectional view of the substrate mixing and capping;

[0037] In the figure: sample management module 1; sample transmission module 2; computer module 3; host module 4; reagent disk module 5; sample needle module 6; arm group module 7; magnetic separation module 8; photometry module 9; cup arrangement module 10; reaction cup 11; magnetic separation bottom plate assembly 12; magnetic separation pot assembly 13; magnetic separation aspiration needle assembly 14; magnetic separation mixing assembly 15; magnetic separation base 16; magnetic separation disk cover 17; rotating disk 18; rotating disk bearing 19; main drive pulley 20; main drive motor 21; elevator 1 22; aspiration needle fixing plate 23; C-shaped fixing plate 24; cleaning liquid mixing column 25; substrate mixing column 26; elevator 2 27; cleaning liquid mixing motor 28; substrate mixing motor 29; reaction cup placement slot 30; fixed track 1 31; sliding track 1 32; lifting motor 1 33; lifting worm 1 34; cup changing mouth 35; cleaning liquid injection needle 36; cleaning liquid mixing pressure cover assembly 37; substrate mixing pressure cover assembly 38; cleaning liquid aspiration needle assembly 39; cleaning liquid injection needle assembly 40; cleaning liquid aspiration needle 41; mounting seat 42; bearing 43; rotating hollow shaft 44; return spring 45; pressure cover head 46; guide pin 47; guide groove 48; lifting worm 2 49; substrate aspiration needle assembly 50; substrate mixing pressure cover 51; substrate aspiration needle 52; stabilizing seat 53; cleaning liquid mixing synchronous belt 54; substrate mixing synchronous belt 55; cleaning liquid mixing driven wheel 56; cleaning liquid mixing tensioning wheel 57; substrate mixing driven wheel 58; substrate mixing synchronous belt 59; substrate mixing tensioning wheel 60; fixed track 2 61; sliding track 2 62; lifting motor 2 63. DETAILED DESCRIPTION

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0039] A high-speed, fully automatic chemiluminescence immunoassay analyzer includes a sample management module 1, a sample transmission module 2, a computer module 3, and a host module 4. A sample is placed in the sample management module 1, and is further transmitted to the host module 4 by the sample transmission module 2, and is controlled and detected by the computer module 3. The host module 4 includes a reagent disk module 5, a sample needle module 6, two arm group modules 7, a magnetic separation module 8, a photometry module 9, and a cup arrangement module 10. The reaction cup 11 is introduced into the sample needle module 6 through the cup arrangement module 10. Further, the reagent is taken out from the reagent disk module 5 and added to the reaction cup 11 of the sample needle module 6. Reaction incubation is performed in the reaction cup 11 of the sample needle module 6. After the reaction incubation is completed, one of the arm group modules 7 transports the reaction cup 11 of the sample needle module 6 to the magnetic separation module 8. After the magnetic separation module 8 completes the operation, the other arm group module 7 transports the reaction cup 11 to the photometry module 9 for detection.

[0040] The magnetic separation module 8 includes a magnetic separation base plate assembly 12, on the upper top surface of which a magnetic separation pot assembly 13 and a magnetic separation pipette needle assembly 14 are provided. A magnetic separation mixing assembly 15 is provided below the magnetic separation pot assembly 13. The magnetic separation pot assembly 13 includes a magnetic separation base 16, a magnetic separation disk cover 17, a rotating disk 18, a rotating disk bearing 19, a main drive pulley 20 and a main drive motor 21. The magnetic separation pipette needle assembly 14 includes an elevator 1 22 and a pipette needle fixing plate 23. The magnetic separation mixing assembly 15 includes a C-shaped fixing plate 24, three cleaning liquid mixing columns 25, a substrate mixing column 26, an elevator 2 27, a cleaning liquid mixing motor 28, and a substrate mixing motor 29.

[0041] The magnetic separation base 16 is fixed to the upper surface of the magnetic separation base assembly 12. A rotating disk bearing 19 is provided on the magnetic separation base 16. The upper end of the rotating disk bearing 19 is fixedly connected to the rotating disk 18, and the lower end of the rotating disk bearing 19 is fixedly connected to the main drive pulley 20. A main drive motor 21 is fixed to the lower surface of the magnetic separation base assembly 12, and the main drive motor 21 and the main drive pulley 20 are in driving connection. When the main drive motor 21 is in operation, it drives the rotating disk 18 to rotate about its axis. The rotating disk 18 is provided with 36 cuvette placement slots 30 evenly distributed in a circular array. Cuvettes 11 are placed in the cuvette placement slots 30. Each cuvette placement slot 30 represents a workstation. The rotating disk 18 rotates clockwise, passing through one workstation at a time.

[0042] The pipette needle fixing plate 23 is located directly above the magnetic separation disc cover 17, and a lift 22 is provided between the magnetic separation bottom plate assembly 12 and the pipette needle fixing plate 23. The lift 22 includes a fixed rail 31, a sliding rail 32 and a lifting motor 33. The fixed rail 31 is fixedly arranged on the magnetic separation bottom plate assembly 12, and the lifting motor 33 is fixedly arranged below the fixed rail 31. The sliding rail 32 is in a limited fit with the fixed rail 31, and the sliding rail 32 can slide up and down along the fixed rail 31. The upper end of the sliding rail 32 is fixedly connected to the pipette needle fixing plate 23, and the lower end of the sliding rail 32 is provided with a lifting worm 34, which is connected to the lifting motor 33. Driven by the lifting motor 33, the pipette needle fixing plate 23 can be lifted up and down.

[0043] The magnetic separation disk cover 17 is provided with a cup changing port 35, a cleaning liquid injection needle 36, three cleaning liquid mixing and pressing assemblies 37 and a substrate mixing and pressing assembly 38 arranged in a circular array. The cup changing port 35 is used to replace the reaction cup 11 on the rotating disk 18. As the rotating disk 18 rotates one station each time, there is always a reaction cup placement slot 30 located directly below the cup changing port 35, which facilitates the front and rear arm group modules 7 to replace the corresponding reaction cup 11. A cleaning liquid injection needle 36 is provided at a position one station away from the cup changing port 35, and the cleaning liquid injection needle 36 injects cleaning liquid into the reaction cup 11 that has just been placed. The three cleaning liquid mixing and pressing assemblies 37 are arranged in sequence behind the cleaning liquid injection needle 36.

[0044] The cleaning liquid mixing and capping assembly 37 includes a cleaning liquid aspiration needle assembly 39 and a cleaning liquid injection needle assembly 40. The cleaning liquid aspiration needle assembly 39 includes a cleaning liquid aspiration needle 41. The upper half of the cleaning liquid aspiration needle 41 is fixed to the aspiration needle fixing plate 23, and the lower half of the cleaning liquid aspiration needle 41 can be inserted into the interior of the magnetic separation pot assembly 13. The cleaning liquid aspiration needle 41 can be raised and lowered along with the aspiration needle fixing plate 23. After the rotating disk 18 rotates one station, the lower end of the cleaning liquid aspiration needle 41 is always aligned with the reaction cup 11 of one of the reaction cup placement slots 30, so that the cleaning liquid aspiration needle 41 can absorb cleaning liquid from the corresponding reaction cup 11. The cleaning fluid injection needle assembly 40 includes a cleaning fluid injection needle 36, a mounting seat 42, a bearing 43, a rotating hollow shaft 44, a return spring 45, and a capping head 46. The mounting seat 42 is provided with a bearing 43, the lower end of which is connected to the rotating hollow shaft 44, and the capping head 46 is nested in the lower end of the rotating hollow shaft 44. A return spring 45 is located within the rotating hollow shaft 44, with its two ends abutting the inner wall of the rotating hollow shaft 44 and the top of the capping head 46, respectively. The cleaning fluid injection needle 36 passes through the bearing 43 and the rotating hollow shaft 44 in sequence, and the distal end of the cleaning fluid injection needle 36 is connected to the capping head 46. As the rotating disk 18 rotates one station, the lower end of the capping head 46 is always aligned with one of the cuvette receiving slots 30. The lower end of the capping head 46 abuts the top of the cuvette 11. While the cleaning solution is injected into the cuvette 11 through the capping head 46, the capping head 46 covers the cuvette 11, enabling rotational mixing and effectively preventing spillage of the cleaning solution during the rotational mixing process. To stabilize the rotation of the cuvette 11 during its rotation, a guide pin 47 is provided on the outer wall of the capping head 46. Correspondingly, a guide groove 48 is provided on the side wall of the rotating hollow shaft 44.

[0045] The substrate mixing and pressing cover assembly 38 includes a substrate pipetting needle assembly 50 and a substrate mixing and pressing cover 51, and the substrate pipetting needle assembly 50 and the substrate mixing and pressing cover 51 are one station apart. As the rotating disk 18 rotates one station, the lower end of the substrate pipetting needle assembly 50 and the lower end of the substrate mixing and pressing cover 51 are always aligned with the reaction cup 11 of one of the reaction cup placement slots 30. After the substrate pipetting needle assembly 50 has absorbed the cleaning liquid in the corresponding reaction cup 11, the reaction cup 11 is transferred to the next station, and the substrate mixing and pressing cover 51 covers the reaction cup 11 for self-rotation mixing. The substrate pipetting needle assembly 50 includes a substrate pipetting needle 52 and a stabilizing seat 53. The upper half of the substrate pipetting needle 52 is fixed to the pipetting needle fixing plate 23, and the lower half of the substrate pipetting needle 52 passes through the stabilizing seat 53. The substrate mixing pressure cover 51 includes a bearing 43, a pressure cover head 46 and a return spring 45. The bearing 43 is installed on the magnetic separation disk cover 17. The pressure cover head 46 passes through the bearing 43. A return spring 45 is provided between the bearing 43 and the pressure cover head 46; a guide pin 47 is provided at the bearing 43, and correspondingly, a guide groove 48 is provided at the pressure cover head 46.

[0046] The magnetic separation mixing assembly 15 includes a C-shaped fixed plate 24, three cleaning liquid mixing columns 25, a substrate mixing column 26, a second elevator 27, a cleaning liquid mixing motor 28, and a substrate mixing motor 29. The three cleaning liquid mixing columns 25 and the three cleaning liquid injection needle assemblies 40 correspond one to one and are located on the same vertical axis, and the substrate mixing column 26 and the substrate mixing pressure cover 51 are located on the same vertical axis. The three cleaning liquid mixing columns 25 and the substrate mixing column 25 are arranged in sequence at the C-shaped fixed plate 24, the cleaning liquid mixing column 25 is rotatably connected to the C-shaped fixed plate 24, and the substrate mixing column 26 is rotatably connected to the C-shaped fixed plate 24. In order to drive the cleaning liquid mixing column 25 and the substrate mixing column 26 to rotate, a cleaning liquid mixing synchronous belt 54 is provided between the cleaning liquid mixing motor 28 and the three cleaning liquid mixing columns 25, and a substrate mixing synchronous belt 55 is provided between the substrate mixing motor 29 and the substrate mixing column 26. A cleaning liquid mixing driven wheel 56 is installed at the bottom of the cleaning liquid mixing column 25. A cleaning liquid mixing synchronous belt 54 is installed between the cleaning liquid mixing motor 28 and the three cleaning liquid mixing driven wheels 56. Two cleaning liquid mixing tensioning pulleys 57 are installed on the lower surface of the C-shaped fixed plate 24 and are connected to the cleaning liquid mixing synchronous belt 54. A substrate mixing driven wheel 58 is installed at the bottom of the substrate mixing column 26. A substrate mixing synchronous belt 59 is installed between the substrate mixing motor 29 and the substrate mixing driven wheel 28. Two substrate mixing tensioning pulleys 60 are installed on the lower surface of the C-shaped fixed plate 24 and are connected to the substrate mixing synchronous belt 59. The cleaning liquid mixing motor 28 drives the three cleaning liquid mixing columns 25 to rotate synchronously, while the substrate mixing motor 29 drives the substrate mixing column 26 to rotate. A reaction cup fixing groove 30 is provided at the top of the cleaning liquid mixing column 25 and the top of the substrate mixing column 26. In order to improve the mixing effect caused by rotation, the reaction cup 11 is designed as a square reaction cup 11, and correspondingly, the reaction cup fixing groove 30 is also designed to be square. A lift 27 is provided between the C-shaped fixed plate 24 and the magnetic separation bottom plate assembly 12. The lift 27 includes a fixed rail 2 61, a sliding rail 2 62 and a lifting motor 2 63. The fixed rail 2 61 is fixedly arranged on the magnetic separation bottom plate assembly 12, and the sliding rail 2 62 is fixedly arranged on the C-shaped fixed plate 24. The sliding rail 2 62 and the fixed rail 2 61 are in a limited fit, and the sliding rail 2 62 can slide along the fixed rail 2 61. The lifting motor 2 63 is fixedly arranged at a position below the fixed rail 2 61, and a lifting worm 2 49 is provided at the sliding rail 2 62, and the lifting worm 2 49 is connected to the lifting motor 2 63. Driven by the second lifting motor 63 , the C-shaped fixed plate 24 can be lifted and lowered, and the three cleaning liquid mixing columns 25 and the one substrate mixing column 26 can be lifted and lowered synchronously.

[0047] During operation, the reaction cup 11 is placed into the reaction cup placement slot 30 of the rotating disk 18 from the cup exchange port 35. Then, the rotating disk 18 rotates through one station, and the cleaning liquid injection needle 36 injects cleaning liquid into the reaction cup 11. Furthermore, the rotating disk 18 rotates one station at a time, and the reaction cups 11 are placed on the rotating disk 18 in sequence. The reaction cup 11 rotates clockwise around the rotating disk bearing 19, and the reaction cup 11 reaches the cleaning liquid aspiration needle assembly 39 of the first cleaning liquid mixing and pressing cover assembly 37. At this time, the aspiration needle fixing plate 23 moves downward, and the cleaning liquid aspiration needle 41 is inserted into the reaction cup 11 to absorb the liquid in the reaction cup 11. Further, the rotating disk 18 rotates one station and reaches the cleaning liquid injection needle assembly 40 of the first cleaning liquid mixing and pressing cover assembly 37. At this time, the C-shaped fixed plate 23 moves upward, and the corresponding cleaning liquid mixing column 25 immediately moves up to lift the reaction cup 11. The upper end of the reaction cup 11 presses against the lower end of the corresponding pressure capping head 46, and the cleaning liquid injection needle 36 injects cleaning liquid into the reaction cup 11. The corresponding cleaning liquid mixing column 25 rotates synchronously. After the mixing is completed, the C-shaped fixed plate 23 moves downward, the reaction cup 11 returns to its original position, and moves to the second cleaning liquid mixing pressure capping assembly 37 along with the rotating disk 18. After the second cleaning liquid mixing pressure capping assembly 37 completes the same operation, the reaction cup 11 moves to the third cleaning liquid mixing pressure capping assembly 37 along with the rotating disk 18. After the third cleaning liquid mixing pressure capping assembly 37 completes the same operation, the reaction cup 11 moves to the substrate mixing pressure capping assembly 38 along with the rotating disk 18. At this time, the aspiration needle fixed plate 23 moves downward, and the substrate aspiration needle 52 is inserted into the corresponding reaction cup 11 to aspirate liquid. After the aspiration is completed, the aspiration needle fixed plate 23 moves upward. Next, the cuvette 11 moves one station along with the rotating disk 18. At this point, the C-shaped fixed plate 23 moves upward, and the corresponding substrate mixing column 26 immediately rises, lifting the cuvette 11. The upper end of the cuvette 11 presses against the lower end of the corresponding capping head 46. The substrate mixing column 26 rotates to mix the contents of the cuvette 11. After mixing, the C-shaped fixed plate 23 moves downward and returns to its original position. Finally, the cuvette 11 moves along with the rotating disk 18 to the cup exchange port 35, where it is removed by the armset module 7. Simultaneously, a new cuvette 11 is placed in another armset module 7.

[0048] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A magnetic separation module, characterized in that: It includes a magnetic separation bottom plate assembly, the upper surface of which is provided with a magnetic separation pot assembly and a magnetic separation liquid pipetting needle assembly, and the lower portion of which is provided with a magnetic separation mixing assembly; The magnetic separation pot assembly includes a magnetic separation base, a magnetic separation disk cover, a rotating disk, a rotating disk bearing, a main drive pulley and a main drive motor; the magnetic separation base is fixed to the upper surface of the magnetic separation bottom plate assembly, and a rotating disk bearing is provided on the magnetic separation base, the upper end of the rotating disk bearing is fixedly connected to the rotating disk, the lower end of the rotating disk bearing is fixedly connected to the main drive pulley, the main drive motor is fixed to the lower surface of the magnetic separation bottom plate assembly, and the main drive motor and the main drive pulley are in driving connection; the rotating disk is provided with a number of reaction cup placement slots evenly distributed in a circular array, and the reaction cup placement slots are provided on the rotating disk. A reaction cup is provided in the groove; the magnetic separation disk cover is provided with a cup changing port, a cleaning liquid injection needle, a plurality of cleaning liquid mixing and pressing assemblies and a substrate mixing and pressing assemblies arranged in a circular array in sequence; the cleaning liquid mixing and pressing assemblies include a cleaning liquid aspiration needle assembly and a cleaning liquid injection needle assembly, and the substrate mixing and pressing assemblies include a substrate aspiration needle assembly and a substrate mixing and pressing assemblies; as the rotating disk rotates through several stations, the cup changing port, the cleaning liquid injection needle, the cleaning liquid aspiration needle assembly, the cleaning liquid injection needle assembly, the substrate aspiration needle assembly and the substrate mixing and pressing assemblies are always located directly above one of the reaction cup placement grooves; The magnetic separation pipetting needle assembly includes a lifter 1 and a pipetting needle fixing plate, a lifter 1 is provided between the magnetic separation bottom plate assembly and the pipetting needle fixing plate, and the pipetting needle fixing plate is located directly above the magnetic separation pot assembly; the cleaning liquid pipetting needle assembly includes a cleaning liquid pipetting needle, and the substrate pipetting needle assembly includes a substrate pipetting needle, and the upper half of the cleaning liquid pipetting needle and the upper half of the substrate pipetting needle are both fixed to the pipetting needle fixing plate; The magnetic separation mixing assembly includes a C-shaped fixed plate, a plurality of cleaning liquid mixing columns, a substrate mixing column, a second elevator, a cleaning liquid mixing motor, and a substrate mixing motor; the plurality of cleaning liquid mixing columns and the substrate mixing columns are arranged in sequence on the C-shaped fixed plate, the plurality of cleaning liquid mixing columns and the plurality of cleaning liquid injection needle assemblies correspond one to one and are located on the same vertical axis, and the substrate mixing column and the substrate mixing gland are located on the same vertical axis; a cleaning liquid mixing synchronous belt is provided between the cleaning liquid mixing motor and the plurality of cleaning liquid mixing columns, and a substrate mixing synchronous belt is provided between the substrate mixing motor and the substrate mixing column; a second elevator is provided between the C-shaped fixed plate and the magnetic separation bottom plate assembly; The cleaning liquid mixing column is rotatably connected to the C-shaped fixed plate, and a reaction cup fixing groove is provided on the top of the cleaning liquid mixing column; a cleaning liquid mixing driven wheel is provided at the bottom of the cleaning liquid mixing column, and a cleaning liquid mixing synchronous belt is provided between the cleaning liquid mixing motor and the plurality of cleaning liquid mixing driven wheels; a plurality of cleaning liquid mixing tensioning wheels are provided on the lower bottom surface of the C-shaped fixed plate, and the plurality of cleaning liquid mixing tensioning wheels are connected to the cleaning liquid mixing synchronous belt; The substrate mixing column is rotatably connected to the C-shaped fixed plate, and a reaction cup fixing groove is provided on the top of the substrate mixing column; a substrate mixing driven wheel is provided at the bottom of the substrate mixing column, and a substrate mixing synchronous belt is provided between the substrate mixing motor and the plurality of substrate mixing driven wheels; a plurality of substrate mixing tensioning wheels are provided on the lower bottom surface of the C-shaped fixed plate, and the plurality of substrate mixing tensioning wheels are connected to the substrate mixing synchronous belt.

2. A magnetic separation module according to claim 1, characterized in that: The cleaning liquid aspiration needle assembly includes a cleaning liquid aspiration needle, the upper half of which is fixed to a aspiration needle fixing plate, and the lower half of which can be inserted into the interior of the magnetic separation pot assembly; as the rotating disk rotates several positions, the lower end of the cleaning liquid aspiration needle is always aligned with one of the reaction cup placement slots.

3. The magnetic separation module according to claim 1, characterized in that: The cleaning liquid injection needle assembly includes a cleaning liquid injection needle, a mounting seat, a bearing, a rotating hollow shaft, a return spring and a pressure head; a bearing is provided at the mounting seat, the lower end of the bearing is connected to the rotating hollow shaft, the lower end of the rotating hollow shaft is nested with the pressure head, a return spring is provided inside the rotating hollow shaft, and the two ends of the return spring respectively abut the inner wall of the rotating hollow shaft and the top of the pressure head; the cleaning liquid injection needle passes through the bearing and the rotating hollow shaft in sequence, and the end of the cleaning liquid injection needle is connected to the pressure head; a guide pin is provided on the outer wall of the pressure head, and correspondingly, a guide groove is provided on the side wall of the rotating hollow shaft; as the rotating disk rotates through several stations, the lower end of the pressure head is always aligned with one of the reaction cup placement slots.

4. The magnetic separation module according to claim 1, characterized in that: The substrate pipetting needle assembly includes a substrate pipetting needle and a stabilizing seat. The upper half of the substrate pipetting needle is fixed to the pipetting needle fixing plate, and the lower half of the substrate pipetting needle passes through the stabilizing seat. As the rotating disk rotates through several stations, the lower end of the substrate pipetting needle is always aligned with one of the reaction cup placement slots.

5. The magnetic separation module according to claim 1, characterized in that: The substrate mixing cover includes a bearing, a cover head and a return spring. The bearing is installed on the magnetic separation disc cover, the cover head passes through the bearing, and a return spring is provided between the bearing and the cover head; a guide pin is provided at the bearing, and correspondingly, a guide groove is provided at the cover head.

6. The magnetic separation module according to claim 1, characterized in that: Elevator 1 includes a fixed rail 1, a sliding rail 1 and a lifting motor 1; the fixed rail 1 is fixedly arranged on the magnetic separation base plate assembly, the lifting motor 1 is fixedly arranged below the fixed rail 1, the sliding rail 1 is in a limited fit with the fixed rail 1, and the sliding rail 1 can slide up and down along the fixed rail 1; the upper end of the sliding rail 1 is fixedly connected to the pipette needle fixing plate, and the lower end of the sliding rail 1 is provided with a lifting worm 1, and the lifting worm 1 is connected to the lifting motor 1.

7. The magnetic separation module according to claim 1, characterized in that: Elevator 2 includes fixed rail 2, sliding rail 2 and lifting motor 2; the fixed rail 2 is fixedly set on the magnetic separation base plate assembly, the sliding rail 2 is fixedly set on the C-shaped fixed plate, the sliding rail 2 and the fixed rail 2 are in a limited fit, and the sliding rail 2 can slide along the fixed rail 2; the lifting motor 2 is fixedly set at the lower position of the fixed rail 2, and the sliding rail 2 is provided with a lifting worm 2, and the lifting worm 2 is connected to the lifting motor 2.

8. A high-speed fully automatic chemiluminescence immunoassay analyzer, characterized in that: Comprising the magnetic separation module according to any one of claims 1 to 7.

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