In-situ sampling ionization spray kit for gas and aerosol samples

Abstract

The invention relates to the technical field of mass spectrometer analysis, in particular to an in-situ sampling ionization spray kit for gas and aerosol samples, which comprises a kit body, a sampling film and a capillary tube. A through hole and a liquid adding groove are respectively formed in the box body, the liquid adding groove is communicated with the through hole, the liquid adding groove is used for dropwise adding a chromatography solution, and the through hole is used for being communicated with a sampling pump; the sampling film is arranged in the through hole and is used for bearing an aerosol sample; the capillary tube is arranged on the box body, one end of the capillary tube extends into the through hole, and the box body is used for providing high-voltage electric connection during sample analysis, so that an analysis solution enters the capillary tube from one end of the capillary tube in an electrified manner and is sprayed out from the other end of the capillary tube to form mist spray. The method has the effect of conveniently detecting the non-volatile substances in the aerosol form.

Description

Technical Field

[0001] The present application relates to the technical field of mass spectrometry instrument analysis, and in particular to an in-situ sampling ionization spray kit for gas and aerosol samples. Background Art

[0002] At present, in-situ ionization technology is a general term for a series of mass spectrometry analysis methods, which allow samples to be ionized directly or with only a very small amount of pretreatment under atmospheric pressure and room temperature, and then analyzed by mass spectrometry. The development of this technology has greatly simplified the sample pretreatment steps, making mass spectrometry analysis faster and easier, and can be used for on-site analysis. Types of in-situ ionization techniques include desorption electrospray ionization (DESI), direct analysis in real time (DART), low temperature plasma (LTP), paper spray ionization (PS), and microtube paper spray ionization (PCS), all of which can achieve direct analysis of samples at room temperature and differential pressure.

[0003] In recent years, environmental pollution issues have received more and more attention. For this reason, my country's Ministry of Ecology and Environment has successively issued relevant testing standards and proposed a series of relevant testing methods for volatile organic compounds (VOCs), semi-volatile organic compounds (SVOCs), etc. In related technologies, adsorbents or adsorbent liquids are usually used for sampling, and then the samples are sent back to the laboratory for detection using gas chromatography-mass spectrometry. Although this method has high sensitivity, the entire detection cycle is long, and after sample collection, complex pre-treatment steps such as extraction and concentration are required to complete the analysis. In this regard, in some cases, staff will use portable mass spectrometers for on-site testing, but the detection range of portable mass spectrometers is mainly limited to volatile and semi-volatile organic compounds, and there are still certain limitations for on-site rapid detection of non-volatile substances in aerosol form.

[0004] Therefore, there is an urgent need for an in-situ sampling ionization spray kit for gas and aerosol samples. Summary of the invention

[0005] In order to facilitate the detection of non-volatile substances in the form of gas and aerosol, the present application provides an in-situ sampling ionization spray kit for gas and aerosol samples.

[0006] The present application provides an in-situ sampling ionization spray kit for gas and aerosol samples, which adopts the following technical solution: An in-situ sampling ionization spray kit for gas and aerosol samples, comprising: A box body, wherein a through hole is formed on the box body, and the through hole is used to communicate with a sampling pump; A sampling membrane is arranged in the through hole, and the sampling membrane is used to carry gas and aerosol samples; A capillary is arranged on the box body, one end of the capillary is in contact with the sampling membrane, and the other end of the capillary extends out of the box body. The box body is used to provide high-voltage electrical connection when performing sample analysis, so that the analysis solution is charged and enters the capillary from one end of the capillary, and is ejected from the other end of the capillary to form a spray.

[0007] By adopting the above technical solution, when it is necessary to detect the non-volatile substances in the form of gas and aerosol, the sampling pump works to extract the gas in the external environment into the through hole, so that the gas passes through the sampling membrane, so as to achieve the effective collection and enrichment of the gas or aerosol sample. Then, the chromatography solution is dripped onto the sampling membrane, and the chromatography solution can dissolve the test object carried on the sampling membrane to form an analysis solution. Finally, the high voltage is connected to the box body, so that the analysis solution is charged, and the analysis solution enters the capillary from one end of the capillary and is ejected from the other end of the capillary to form a spray, so as to facilitate the ionization of the sample, to ensure that the sample can obtain efficient ionization and good transmission in mass spectrometry analysis, and then facilitate mass spectrometry analysis, so as to achieve the purpose of detecting the non-volatile substances in the form of gas and aerosol.

[0008] Optionally, the box body includes an upper shell and a lower shell, the upper shell is detachably connected to the lower shell, the through holes are respectively arranged through the upper shell and the lower shell, and the sampling membrane and the capillary are respectively arranged between the upper shell and the lower shell.

[0009] By adopting the above technical solution, the box body of the test kit is composed of an upper shell and a lower shell, which are detachably connected, thereby facilitating the assembly of the sampling membrane and the capillary tube between the upper shell and the lower shell.

[0010] Optionally, a liquid adding tank is provided on the upper shell body, chromatography paper is arranged between the upper shell body and the lower shell body, one end of the chromatography paper extends into the liquid adding tank, the other end of the chromatography paper is overlapped with the sampling membrane, and the capillary is connected to the chromatography paper.

[0011] By adopting the above technical solution, the chromatography paper is arranged between the upper shell and the lower shell, and one end of the chromatography paper extends into the liquid adding tank, and the other end extends into the through hole and contacts the sampling membrane, so that when the chromatography solution is dripped into the liquid adding tank, the chromatography solution can be transmitted along the chromatography paper to achieve effective transmission and uniform distribution of the chromatography solution, which is beneficial to improve the extraction efficiency and analysis accuracy of the sample.

[0012] Optionally, a receiving groove is provided on the upper shell, the receiving groove is located between the liquid adding groove and the through hole, a conductive member is embedded in the receiving groove, the conductive member contacts the chromatography paper, and the conductive member is used to connect high voltage electricity.

[0013] By adopting the above technical solution, a receiving groove is opened on the upper shell of the reagent kit, and a conductive part is embedded in the receiving groove and contacts the chromatography paper. Therefore, when the conductive part is connected to high voltage electricity, the high voltage electricity can be transmitted to the analysis solution through the conductive part and the chromatography paper in sequence, so that the analysis solution is charged and the analysis solution forms a stable electrospray under the action of the electric field, which is beneficial to improve the stability and efficiency of sample analysis.

[0014] Optionally, a guiding slope is provided on the upper shell body, the guiding slope is located on a side of the liquid adding groove away from the through hole, and the guiding slope is inclined toward the box body.

[0015] By adopting the above technical solution, the setting of the guiding slope can promote the chromatography solution to flow into the liquid adding tank more smoothly, so that the chromatography solution flows from the liquid adding tank to the through hole, thereby helping to reduce the possibility of the chromatography solution falling outside the liquid adding tank, resulting in waste of the chromatography solution.

[0016] Optionally, a support member is disposed in the through hole, the sampling membrane is disposed on the support member, and the support member is hollowed out.

[0017] By adopting the above technical solution, the sampling membrane is set on the support, and the support is hollowed out, so that on the one hand, it is convenient to use the support to support the sampling membrane to effectively prevent the sampling membrane from being deformed or damaged during use, and on the other hand, the support is not easy to hinder the transmission of the sample, so that the gas or aerosol sample can pass through the sampling membrane smoothly, so that the gas or aerosol sample can be enriched on the sampling membrane. When the desorption solution is added, the hollow support allows the solution to evenly contact the sample on the sampling membrane, which is conducive to improving the desorption efficiency and the sensitivity in the subsequent analysis process.

[0018] Optionally, the sampling membrane is made of polymer material.

[0019] By adopting the above technical solution, the sampling membrane is made of polymer material, which can improve the chemical stability and durability of the sampling membrane while ensuring that the sampling membrane has good filtering performance. The specific material of the sampling membrane can be designed according to actual needs, so that it is suitable for the collection of various gas or aerosol samples, expanding the application scope of the test kit.

[0020] Optionally, a connecting piece is provided in the through hole, an air flow hole is opened in the connecting piece, the air flow hole is communicated with the through hole, and one end of the connecting piece extends out of the through hole and is used to connect a sampling pump.

[0021] By adopting the above technical solution and setting of the connecting piece, the sampling pump can be conveniently connected to the through hole, so that when the sampling pump is working, the sampling pump can extract gas and aerosol samples from the external environment through the air flow holes and the through hole, so as to realize the effective collection of gas and aerosol samples, thereby improving the sampling efficiency to a certain extent.

[0022] Optionally, a bracket is provided on the box body, a mounting hole is formed on the bracket, the mounting hole is communicated with the through hole, and the capillary is inserted into the mounting hole and fits against an inner wall of the mounting hole.

[0023] By adopting the above technical solution, the capillary is inserted into the mounting hole, so that the bracket can support the capillary to correctly fix the capillary in a predetermined position, which is beneficial to avoid the angle of the capillary from being offset, and further beneficial to improving the stability and reliability of the capillary, thereby enhancing the accuracy during sample analysis.

[0024] Optionally, a protective member is provided on one end of the box body close to the capillary, and the protective member is used to protect the capillary.

[0025] By adopting the above technical solution and setting the protective part, the capillary can be effectively protected from damage by external forces, thereby ensuring the stability and reliability of the capillary during use and ensuring the normal progress of the ionization spray process.

[0026] In summary, the present application includes at least one of the following beneficial technical effects: 1. Through the cooperation of the box body, sampling membrane and capillary, the gas or aerosol sample can be quickly enriched on the sampling membrane on site and extracted by the chromatography solution to form an analytical solution. When the box body is connected to high voltage, the analytical solution can enter the capillary and spray out from one end of the capillary to form a spray, which is convenient for analysis by mass spectrometer, achieving the purpose of detecting non-volatile substances in the form of gas or aerosol, greatly shortening the detection time and improving the detection efficiency; 2. Through the cooperation of the box body, sampling membrane, capillary tube and chromatography paper, the chromatography solution can be transferred along the chromatography paper to the sampling membrane, which is beneficial to improve the extraction efficiency and analysis accuracy of the sample; 3. The sampling membrane can be arranged on the support member through the hollowing out of the support member, so that on the one hand, the sampling membrane can be supported by the support member to effectively prevent the sampling membrane from being deformed or damaged during use, and on the other hand, the support member is not easy to hinder the transmission of the sample, so that the gas or aerosol sample can pass through the sampling membrane smoothly, thereby facilitating the gas or aerosol sample to be enriched on the sampling membrane; 4. The capillary is arranged through the mounting hole so that the bracket can support the capillary to correctly fix the capillary in a predetermined position, thereby helping to avoid the angle of the capillary from being offset, thereby helping to improve the stability and reliability of the capillary and enhancing the accuracy during sample analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the overall structure of an in-situ sampling ionization spray kit for gas and aerosol samples in an embodiment of the present application.

[0028] Figure 2 It is a top view of an in-situ sampling ionization spray kit for gas and aerosol samples in an embodiment of the present application.

[0029] Figure 3 It is a schematic diagram of the structure of the capillary and chromatography paper in the embodiment of the present application.

[0030] Figure 4 is along Figure 2 Section view along line AA.

[0031] Figure 5 A top view of an in-situ sampling ionization spray kit for gas and aerosol samples without a sampling membrane.

[0032] Figure 6 It is the mass spectrum of nonanoic acid vanillamide in the test aerosol in the example of the present application.

[0033] Description of reference numerals: 1. Box body; 11. Through hole; 12. Liquid adding tank; 13. Upper shell; 131. Guide slope; 132. Accommodating tank; 133. Conductive member; 14. Lower shell; 15. Bracket; 151. First supporting part; 152. Second supporting part; 153. Mounting hole; 16. Protective member; 161. Protective antenna; 2. Sampling membrane; 3. Capillary; 4. Chromatography paper; 5. Support member; 6. Connecting member; 61. Air flow hole. DETAILED DESCRIPTION

[0034] The following is combined with Figure 1-6 This application is described in further detail.

[0035] The embodiment of the present application discloses an in-situ sampling ionization spray kit for gas and aerosol samples.

[0036] It should be noted that, in the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicating orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0037] Reference Figure 1 and Figure 2 An in-situ sampling ionization spray kit for gas and aerosol samples includes a box body 1, a sampling membrane 2 and a capillary 3. The box body 1 is provided with a through hole 11 and a liquid adding groove 12, the liquid adding groove 12 is connected to the through hole 11, and the through hole 11 is used to connect with the sampling pump, and the liquid adding groove 12 is used to drip the chromatography solution. The sampling membrane 2 is arranged in the through hole 11, and is used to carry the aerosol sample. The capillary 3 is arranged on the box body 1, and one end of the capillary 3 extends into the through hole 11. The box body 1 is used to provide a high-voltage electrical connection when performing sample analysis, so that the analysis solution is charged and enters the capillary 3 from one end of the capillary 3, and is sprayed out from the other end of the capillary 3 to form a spray.

[0038] Reference Figure 1 The box body 1 includes an upper shell 13 and a lower shell 14. The upper shell 13 and the lower shell 14 are detachably connected. The through hole 11 is respectively set through the upper shell 13 and the lower shell 14, and in this embodiment, the diameter of the through hole 11 is 1.0 cm.

[0039] The liquid adding tank 12 is arranged on the upper shell 13, and the liquid adding tank 12 passes through the upper shell 13. A guiding slope 131 is arranged on the upper shell 13. The guiding slope 131 is located on the side of the liquid adding tank 12 away from the through hole 11. One end of the guiding slope 131 is inclined toward the bottom of the upper shell 13, so as to facilitate guiding the added chromatography solution into the liquid adding tank 12.

[0040] Reference Figure 1 and Figure 3 , a chromatography paper 4 is arranged between the upper shell 13 and the lower shell 14. The chromatography paper 4 is made of synthetic fiber material and has a thickness of 0.5 mm. In this embodiment, the speed of the chromatography solution passing through the chromatography paper 4 is 200 mm / 30 min, so that the chromatography paper 4 has good liquid absorption and uniform transmission characteristics, so that the chromatography solution can be carried by the chromatography paper 4. When the upper shell 13 and the lower shell 14 are connected and fixed, the chromatography paper 4 is clamped between the upper shell 13 and the lower shell 14 to prevent the chromatography paper 4 from moving.

[0041] Reference Figure 4The upper shell 13 is provided with a receiving groove 132, which is located between the liquid adding groove 12 and the through hole 11, and a conductive member 133 is embedded in the receiving groove 132, and the conductive member 133 has good conductivity, so that it is convenient to use the conductive member 133 to connect high voltage electricity. The conductive member 133 is in contact with the chromatography paper 4, and when the conductive member 133 is connected to the high voltage electricity, the high voltage electricity can be transmitted to the analysis solution through the conductive member 133 and the chromatography paper 4 in sequence.

[0042] In this embodiment, the conductive member 133 is a stainless steel bead, so that the conductive member 133 is not easy to react with the analysis solution, which helps to maintain the purity of the analysis solution.

[0043] Reference Figure 4 and Figure 5 A support member 5 is fixedly connected to the lower shell 14, the support member 5 is located in the through hole 11 and is arranged close to the upper shell 13, and the sampling membrane 2 is placed on the support member 5 and overlaps with the chromatography paper 4, so that when the chromatography solution is dripped into the liquid adding tank 12, the chromatography solution can be transferred along the chromatography paper 4 to the sampling membrane 2 to contact the analyte on the sampling membrane 2 to form an analysis solution.

[0044] In this embodiment, the diameter of the sampling membrane 2 is larger than the diameter of the through hole 11 , and the portion of the sampling membrane 2 larger than the through hole 11 is clamped between the upper shell 13 and the lower shell 14 , which is beneficial to improve the stability of the sampling membrane 2 .

[0045] The support member 5 is hollowed out, so that on the one hand, it is convenient to use the support member 5 to support the sampling membrane 2, so as to effectively prevent the sampling membrane 2 from being deformed or damaged during use; on the other hand, the support member 5 is not easy to hinder the transmission of the sample, so that the gas or aerosol sample can pass through the sampling membrane 2 smoothly, so as to enrich the gas or aerosol sample on the sampling membrane 2.

[0046] Reference Figure 4 The sampling membrane 2 is made of polymer material, with a diameter of 13 mm and a pore size of 0.8 μm. In this embodiment, the sampling membrane 2 can be made of glass fiber, polypropylene fiber, polytetrafluoroethylene or polystyrene material, which can be selected according to the actual detection requirements and the properties of the target pollutants, so as to ensure that the sampling membrane 2 has good filtering performance while improving the chemical stability and durability of the sampling membrane 2.

[0047] Reference Figure 1 and Figure 4A bracket 15 is provided on one side of the box body 1 near the through hole 11, and the bracket 15 includes a first support portion 151 and a second support portion 152. The first support portion 151 is mounted on the upper shell 13, and the second support portion 152 is mounted on the lower shell 14. A mounting hole 153 is provided between the first support portion 151 and the second support portion 152, and the mounting hole 153 is connected to the through hole 11.

[0048] The capillary 3 is inserted into the mounting hole 153 and fits against the inner wall of the mounting hole 153, and one end of the capillary 3 is inserted into the chromatography paper 4, and the other end of the capillary 3 extends out of the mounting hole 153, so that the capillary 3 can be supported by the first supporting portion 151 and the second supporting portion 152, so as to correctly fix the capillary 3 at a predetermined position, thereby facilitating avoiding the angle of the capillary 3 from being offset.

[0049] Reference Figure 1 A protective member 16 is fixedly connected to one end of the box body 1 near the capillary 3, and the protective member 16 includes a plurality of protective antennae 161. In this embodiment, four protective antennae 161 are provided, two of which are fixedly connected to the upper shell 13, and the other two of which are fixedly connected to the lower shell 14. The four protective antennae 161 are distributed in a rectangular shape and are arranged around the capillary 3, thereby effectively preventing external forces from causing damage to the capillary 3, ensuring the safety of the reagent kit during use and transportation, and improving the reliability and service life of the overall device.

[0050] Reference Figure 4 , a connector 6 is provided in the through hole 11, an air flow hole 61 is provided in the connector 6, and the air flow hole 61 is communicated with the through hole 11. In this embodiment, the connector 6 and the through hole 11 are connected by a threaded connection, so that the connector 6 can be easily disassembled and maintained. One end of the connector 6 extends out of the through hole 11 and is used to connect the sampling pump, so that the sampling pump can be easily connected to the through hole 11, so that when the sampling pump is working, the sampling pump can extract gas and aerosol samples in the external environment through the air flow hole 61 and the through hole 11, so as to achieve effective collection of gas and aerosol samples, thereby improving the sampling efficiency to a certain extent.

[0051] The implementation principle of an in-situ sampling ionization spray kit for gas and aerosol samples in an embodiment of the present application is: when it is necessary to detect the gas and aerosol samples, first connect the sampling pump using the connector 6, and then start the sampling pump to extract a certain volume of gas in the external environment into the through hole 11, so that the gas passes through the sampling membrane 2, so that the gas and aerosol samples are enriched on the sampling membrane 2.

[0052] Next, separate the sampling pump from the connector 6, and add 10-500 μL of chromatography solution into the liquid adding tank 12. The chromatography solution can contact the analyte on the sampling membrane 2 through the chromatography paper 4. Wait for 0.5-5 minutes to allow the chromatography solution to fully contact the analyte, thereby dissolving the analyte to form an analytical solution.

[0053] Finally, the reagent kit is inserted into the mass spectrometer, and the conductive member 133 is connected to high voltage, so that the analytical solution is charged, and the analytical solution enters the capillary 3 from one end of the capillary 3, and then sprays out from the other end of the capillary 3 to form an electrospray, so that the analyte enters the mass analyzer in the mass spectrometer for analysis, thereby realizing the detection of the analyte.

[0054] In this application, a fast and simple in-situ sampling ionization spray kit is developed by integrating sample collection, concentration and ionization. Combined with a portable small mass spectrometer, the analysis of organic matter in gas or aerosol can be completed on-site within 10 minutes.

[0055] The following is an example of detecting nonanoic acid vanillylamide in aerosol. The specific steps are as follows: The sampling pump was used to pump air at a flow rate of 6 L / min for 1 min (sampling volume 6 L); 300 μL of acetonitrile solution was then added to the chromatography paper 4 and waited for 4 min; finally, the kit was inserted into a small in-situ ionization mass spectrometer for detection. The mass spectrum obtained was shown in the figure below. Figure 6 shown.

[0056] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. An in-situ sampling ionization spray kit for gas and aerosol samples, characterized in that: include: A box body (1), wherein the box body (1) is provided with a through hole (11), and the through hole (11) is used to communicate with a sampling pump; A sampling membrane (2) is arranged in the through hole (11), and the sampling membrane (2) is used to carry gas and aerosol samples; A capillary tube (3) is arranged on the box body (1), one end of the capillary tube (3) contacts the sampling membrane (2), and the other end of the capillary tube (3) extends out of the box body (1). The box body (1) is used to provide a high-voltage electrical connection when performing sample analysis, so that the analysis solution is charged and enters the capillary tube (3) from one end of the capillary tube (3) and is sprayed out from the other end of the capillary tube (3) to form a spray.

2. The in-situ sampling ionization spray kit for gas and aerosol samples according to claim 1, characterized in that: The box body (1) comprises an upper shell (13) and a lower shell (14), wherein the upper shell (13) is detachably connected to the lower shell (14), the through hole (11) is respectively arranged to penetrate the upper shell (13) and the lower shell (14), and the sampling membrane (2) and the capillary tube (3) are respectively arranged between the upper shell (13) and the lower shell (14).

3. The in-situ sampling ionization spray kit for gas and aerosol samples according to claim 2, characterized in that: The upper shell (13) is provided with a liquid adding tank (12), and a chromatography paper (4) is arranged between the upper shell (13) and the lower shell (14). One end of the chromatography paper (4) extends into the liquid adding tank (12), and the other end of the chromatography paper (4) is arranged to overlap with the sampling membrane (2), and the capillary (3) is connected to the chromatography paper (4).

4. The in-situ sampling ionization spray kit for gas and aerosol samples according to claim 3, characterized in that: The upper shell (13) is provided with a receiving groove (132), the receiving groove (132) is located between the liquid adding groove (12) and the through hole (11), a conductive member (133) is embedded in the receiving groove (132), the conductive member (133) is in contact with the chromatography paper (4), and the conductive member (133) is used to connect high voltage electricity.

5. The in-situ sampling ionization spray kit for gas and aerosol samples according to claim 3, characterized in that: A guiding inclined surface (131) is provided on the upper shell (13); the guiding inclined surface (131) is located on a side of the liquid adding groove (12) away from the through hole (11); and the guiding inclined surface (131) is arranged to be inclined toward the inside of the box body (1).

6. The in-situ sampling ionization spray kit for gas and aerosol samples according to claim 1, characterized in that: A support member (5) is arranged in the through hole (11), the sampling membrane (2) is arranged on the support member (5), and the support member (5) is hollowed out.

7. The in-situ sampling ionization spray kit for gas and aerosol samples according to claim 1, characterized in that: The sampling membrane (2) is made of polymer material.

8. The in-situ sampling ionization spray kit for gas and aerosol samples according to claim 1, characterized in that: A connecting piece (6) is arranged in the through hole (11), an air flow hole (61) is opened in the connecting piece (6), the air flow hole (61) is communicated with the through hole (11), and one end of the connecting piece (6) extends out of the through hole (11) and is used for connecting a sampling pump.

9. The in-situ sampling ionization spray kit for gas and aerosol samples according to claim 1, characterized in that: The box body (1) is provided with a bracket (15), the bracket (15) is provided with a mounting hole (153), the mounting hole (153) is communicated with the through hole (11), and the capillary tube (3) is inserted into the mounting hole (153) and fits with the inner wall of the mounting hole (153).

10. The in-situ sampling ionization spray kit for gas and aerosol samples according to claim 1, characterized in that: A protective member (16) is provided on one end of the box body (1) close to the capillary tube (3), and the protective member (16) is used to protect the capillary tube (3).

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