Treatment method for improving germination and saline-alkaline tolerance of gordon euryale seeds

Abstract

The invention discloses a treatment method for improving germination and saline-alkaline tolerance of gordon euryale seeds, and belongs to the field of aquatic plant breeding and planting, the gordon euryale seeds subjected to disinfection treatment are mixed with fine sand, then the sand-buried seeds are put into a breathable woven bag to be stored, and the storage temperature is 4 + / -2 DEG C. According to the storage method for improving germination and saline-alkaline tolerance of the gordon euryale seeds, provided by the invention, the maturity of the seeds can be ensured by controlling the collection time and color; a water separation method is adopted to ensure the quality of mature seeds; potassium permanganate is adopted for disinfection to ensure that the seeds do not mildew; low-temperature sand storage is adopted to ensure seed quality and break dormancy; and the germination rate and the germination potential are further improved by a variable-temperature germination promoting technology after storage. The method provided by the invention is simple to operate, and can be flexibly implemented according to design parameters in combination with actual facility conditions so as to improve the germination and saline-alkaline tolerance of the gordon euryale seeds.

Description

Technical Field

[0001] The invention belongs to the field of aquatic plant breeding and planting, and particularly relates to a treatment method for improving germination and salt-alkali tolerance of Euryale ferox seeds. Background Art

[0002] Wetland is one of the three major ecosystems on the earth, providing a variety of resources for human production, life and leisure. Due to the rise and fall of groundwater levels, climate change and other reasons, some wetlands will inevitably undergo salinization to varying degrees, so saline-alkali wetlands are an important type of wetland. Salt-alkali stress seriously affects the normal growth and development of plants, destroys the physiological functions of plant cells, and affects the normal growth and development of plants. As a common aquatic economic plant in my country, Euryale ferox Salisb not only plays a role in maintaining wetland plant communities, but also can be used as a food source to provide economic income. Euryale ferox Salisb is a plant of the genus Euryale in the Nymphaeaceae family. It is an annual aquatic herb. It is rich in nutrients, contains a large amount of dietary fiber, polyphenols and polysaccharides, and is widely planted.

[0003] The reproduction methods of wetland plants include sexual reproduction, asexual reproduction, and the coexistence of sexual and asexual reproduction. The reproduction method of Gorgon fruit is a coexistence of sexual and asexual reproduction. In recent years, the research on the introduction and transplantation of Gorgon fruit using sexual reproduction has been gradually strengthened. Seed dormancy is a physiological phenomenon in which seeds cannot germinate normally under suitable germination conditions. It is of great significance to the individual survival, species continuation and evolution of plants. Whether seeds can germinate under saline-alkali stress is a prerequisite for the development of plants under saline-alkali conditions, and the most prominent stages of plant development are seed germination and seedling development. These two stages are the most sensitive stages to abiotic stress. The physiological toxicity of alkaline salts to seed germination is mainly manifested in the stress effects of high pH, ​​high osmotic pressure and ion toxicity. Excessive saline-alkali stress will reduce the germination rate of seeds and inhibit the growth of seedlings, causing plant growth retardation.

[0004] It can affect the oxygen and CO in the embryo development environment. 2 Low temperature has a great influence on seed germination potential. Temperature determines whether seeds can germinate, the speed of germination and the time of emergence. Low temperature and sand storage can effectively break the hard dormancy and embryo dormancy of seeds. It is very necessary to use low temperature and sand storage to break seed dormancy.

[0005] The breeding and cultivation of aquatic economic plants is an important part of wetland restoration and wetland ecological industry development in saline-alkali areas. Most of the previous technical research on Gorgon fruit focused on its edible, medicinal and high-yield cultivation, but there was little research on its saline-alkali resistance. Improving the growth of Gorgon fruit in soda saline-alkali wetlands and enhancing its germination ability under saline-alkali conditions are both urgently needed. Storage methods are an important measure that affects seed quality. Summary of the invention

[0006] The purpose of the present invention is to provide a treatment method for improving the germination and salt-alkali tolerance of Gorgon fruit seeds, so as to solve the problems existing in the above-mentioned prior art. The present invention solves the difficulties of low germination and salt-alkali tolerance of existing Gorgon fruit seeds, and performs low-temperature and sand storage composite storage treatment from the perspective of two influencing factors, temperature and humidity of Gorgon fruit seed storage, and improves the germination ability and salt-alkali tolerance of aquatic economic plants Gorgon fruit by regulating the temperature and humidity conditions during Gorgon fruit seed storage, so as to provide support for the promotion and planting of Gorgon fruit in saline-alkali wetlands.

[0007] The technical solution provided by the present invention is:

[0008] A treatment method for improving the germination and salt-alkali tolerance of Euryale ferox seeds comprises mixing the disinfected Euryale ferox seeds with fine sand, then putting the sand-buried seeds into a breathable woven bag for storage at a storage temperature of 4±2°C, and performing temperature-changing to promote germination after the storage is completed.

[0009] Preferably, the Euryale ferox seeds and fine sand are mixed in a volume ratio of (1:1)-(2:1), and the seeds are preferably wrapped or buried.

[0010] Preferably, the water content of the fine sand is controlled at 40±10 wt %, so that the fine sand can be clumped when held by hand and will become loose when released.

[0011] Preferably, the storage time is 7-8 months.

[0012] Preferably, the method for collecting and screening the Euryale ferox seeds is as follows: from mid-September to mid-October, individual sampling is performed, and the seeds with dark purple berries, black, round, and plump seeds are mature and collection begins. The aril of the collected Euryale ferox seeds is removed, and then the floating impurities and shriveled particles are further removed by water selection, and the high-quality and mature Euryale ferox seeds that sink to the bottom are screened.

[0013] Preferably, the disinfection method is: using potassium permanganate solution to disinfect the surface of Gorgon seeds. More preferably, the concentration of the potassium permanganate solution is 0.05-0.1wt%, preferably 0.05wt% potassium permanganate solution.

[0014] Preferably, the method of changing temperature to promote germination is: the seeds are stored in sand at low temperature until April of the following year, the seeds are taken out and the buried sand is sieved out, the seeds are cleaned, the seeds are placed in a bud bed (soil, sand, filter paper), covered with a sealing film, and the humidity of the bud bed is maintained at more than 80%, and then cultured for 24 hours as a cycle, with each cycle being 16℃±2℃ in the dark and then 25℃±5℃ in the light, each for 12±2 hours. When more than 80% of the seeds are white, they can be sown or raised.

[0015] Beneficial effects of the present invention:

[0016] The present invention provides a treatment method for improving the germination and salt-alkali tolerance of Euryale ferox seeds, which can ensure the maturity of seeds by controlling the collection time and color; using a water selection method to ensure the quality of mature seeds; using potassium permanganate disinfection to ensure that the seeds are not moldy; using low temperature and sand storage to ensure seed quality and contact dormancy; and after storage, using a variable temperature germination promotion technology to further improve the germination rate and germination potential. The treatment method provided by the present invention is simple to operate, and can also be flexibly implemented according to design parameters in combination with actual facility conditions to improve the germination and salt-alkali tolerance of Euryale ferox seeds. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0018] Figure 1 This is a real photo of the Euryale ferox plant in the saline-alkali wetland in Experimental Example 3;

[0019] Figure 2 This is a real photo of the Euryale ferox seeds in the saline-alkali wetland in Experimental Example 3. DETAILED DESCRIPTION

[0020] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0021] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0022] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0023] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present application description and examples are exemplary only.

[0024] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0025] The normal temperature of the present invention refers to 25±2°C.

[0026] Example 1

[0027] 1) Seed collection and processing: At the end of September, Gorgon seeds were collected from native plants in the Momong National Nature Reserve in Jilin Province. Through individual sampling, the seeds with dark purple berries, black, round, and plump seeds are mature and can be collected. The aril of the collected Gorgon seeds is removed, and then the floating impurities and shriveled particles are further removed by water selection. The seeds that sink to the bottom are high-quality and mature Gorgon seeds. Seeds with plump morphology and consistent maturity are selected, and the surface of the Gorgon seeds is disinfected with a potassium permanganate solution with a concentration of 0.05wt% for 10 minutes and rinsed clean;

[0028] 2) Storage: After drying in the shade, the seeds are mixed with fine sand that has been sterilized at high temperature (120°C, 20 min, the same below) in a volume ratio of 1:1. The moisture mass ratio of the fine sand is controlled at 40wt%, and then placed in a breathable woven bag to prevent sand from leaking. Store in a 4°C refrigerator until April of the following year.

[0029] Example 2

[0030] The same as Example 1, except that the seeds are dried in the shade and mixed with high temperature sterilized fine sand at a volume ratio of 2:1.

[0031] The present invention considers the two influencing factors of temperature and humidity in seed storage and sets the following comparative examples:

[0032] Comparative Example 1

[0033] 1) Seed collection and processing: same as in Example 1;

[0034] 2) Storage: Use the room temperature dry storage method. The specific operation is: dry the seeds in the shade and place them in a barrel, and store them at room temperature indoors until April of the following year.

[0035] Comparative Example 2

[0036] 1) Seed collection and processing: same as in Example 1;

[0037] 2) Storage: Use the room temperature sand storage method. The specific operation is: dry the seeds in the shade and mix them with sterilized fine sand in a volume ratio of 1:1. The moisture content of the fine sand is controlled at 40wt%. The sand can be formed into a ball when held in the hand, and it will fall apart when loosened. Put the seeds in a breathable woven bag so that no sand leaks out. Store at room temperature until April of the following year.

[0038] Comparative Example 3

[0039] 1) Seed collection and processing: same as in Example 1;

[0040] 2) Storage: Use low-temperature dry storage method. The specific operation is: dry the seeds in the shade and place them in a breathable woven bag, and store them in a refrigerator at 4℃ until April of the following year.

[0041] Comparative Example 4

[0042] 1) Seed collection and processing: same as in Example 1;

[0043] 2) Storage: Use the frozen storage method. The specific operation is: dry the seeds in the shade, put them into a breathable woven bag, and store them in a -2℃ freezer until April of the following year.

[0044] Comparative Example 5

[0045] The same as Example 1, except that the moisture content of the fine sand is controlled at 10%.

[0046] Comparative Example 6

[0047] The same as Example 1, except that the moisture content of the fine sand is controlled at 60%.

[0048] Experimental Example 1

[0049] According to NaHCO 3 :Na 2 CO 3=9:1 molar concentration ratio of soda salt stress treatment solution, 30 stored Euryale seeds were selected from Example 1 and Comparative Examples 1-6, and placed in plastic cups with an inner diameter of 10 cm and a height of 30 cm, respectively, and different concentrations of soda salt solution were added, and clean water was used as a control. Each treatment was repeated 3 times, and the seeds were placed in an HPG-280HX artificial climate incubator (14h light 25°C, 10h dark 18°C) for seed germination experiment. The breakthrough of the seed coat of the seedling was used as a germination sign, and the germination rate and germination potential of Euryale were recorded.

[0050] in:

[0051] Germination rate = number of germinations / number of test seeds × 100%;

[0052] Germination potential = number of germinations within 5 days / number of test seeds × 100%

[0053] Table 1 shows the germination rate of Euryale ferox seeds with different treatments under different soda saline-alkali stresses, and Table 2 shows the germination potential of Euryale ferox seeds with different treatments under different soda saline-alkali stress intensities.

[0054] Table 1

[0055]

[0056] Note: “—” indicates no data

[0057] Table 2

[0058]

[0059] As can be seen from Table 1 and Table 2, under various treatment methods, only the Euryale ferox seeds treated by low-temperature sand storage in Example 1 germinated, and had a certain germination rate under a certain intensity of saline-alkali stress gradient, and the seeds did not germinate under the storage methods of Comparative Examples 1-4. When Euryale ferox seeds are subjected to saline-alkali stress, their ability to resist saline-alkali stress decreases with the increase of saline-alkali stress intensity. The low-temperature sand storage method provided by the present invention creates conditions for germination and growth under saline-alkali habitat conditions by breaking the dormancy of Euryale ferox seeds. The storage method for short-term storage of Euryale ferox seeds should adopt low-temperature sand storage. From the results of Comparative Examples 5 and 6, when low-temperature sand storage is adopted, too high humidity will slightly interfere with germination ability, and too low humidity will limit germination.

[0060] Experimental Example 2

[0061] The same as Example 1, except that the seeds were dried in the shade and mixed with high-temperature sterilized fine sand in a volume ratio of 2:1. The results showed that when the volume ratio of sand to seeds was between 1:1 and 2:1, it had an effect on seed germination, but did not reach a significant effect level.

[0062] Experimental Example 3

[0063] The seeds germinated in Experimental Example 1 were sown in the saline-alkali wetland habitat of Niuxintaobao National Wetland Park in Da'an, Jilin Province (pH 8.11, conductivity 1711 μS / cm, salinity 0.88 ppt). The Euryale ferox grew well and the seeds were plump. Figure 1 This is a real photo of the Euryale ferox plant in the saline-alkali wetland; Figure 2 This is a real photo of Gorgon seeds in saline-alkali wetlands.

[0064] In summary, under the treatment method provided in Example 1 of the present invention, the Gorgon fruit seeds have a certain germination rate under various saline-alkali stress gradients, and the treatment method creates conditions for the germination and growth of Gorgon fruit in saline-alkali habitat conditions.

[0065] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for improving the germination and salt-alkali tolerance of Euryale ferox seeds, characterized in that: The sterilized Euryale ferox seeds are mixed with fine sand, and then the sand-buried seeds are placed in a breathable woven bag for storage at a temperature of 4±2° C. After the storage, the temperature is changed to promote germination.

2. The method for improving the germination and salt-alkali tolerance of Euryale ferox seeds according to claim 1, characterized in that: The euryale seeds and fine sand are mixed in a volume ratio of (1:1)-(2:1).

3. The method for improving the germination and salt-alkali tolerance of Euryale ferox seeds according to claim 1, characterized in that: The water mass ratio of the fine sand is controlled at 40±10wt%.

4. The method for improving the germination and salt-alkali tolerance of Euryale ferox seeds according to claim 1, characterized in that: The storage time is 7-8 months.

5. The method for improving the germination and salt-alkali tolerance of Euryale ferox seeds according to claim 1, characterized in that: The disinfection treatment method is: using potassium permanganate solution to disinfect the surface of Euryale ferox seeds.

6. The method for improving the germination and salt-alkali tolerance of Euryale ferox seeds according to claim 5, characterized in that: The concentration of the potassium permanganate solution is 0.05-0.1 wt %.

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