Microorganism detection device and microorganism detection method

Abstract

The invention relates to the technical field of microbiological detection, and particularly provides a microbiological detection device and a microbiological detection method. The microbiological detection device comprises an outer cylinder, an inner cylinder is fixed in the outer cylinder, a lifting frame is placed in the inner cylinder, a plurality of culture dishes composed of culture bases and culture covers are placed on the lifting frame, the culture covers are buckled on the culture bases, a counterweight assembly is arranged at the bottom of the lifting frame, and the counterweight assembly is connected with the culture dishes. When the lifting frame carries the culture dish to be placed in the inner cylinder body, the lifting frame descends relative to the balance weight assembly under the influence of the load of the culture dish, the culture cover is triggered to be opened upwards through the balance weight assembly, and when the culture dish is stored in the inner cylinder body before being used, not only is internal sterilization completed, but also the culture dish can be used as all other culture dishes. And the outside of the culture dish is sterilized for multiple times, so that the culture dish for inspection achieves the purpose of being used and sterilized at any time before a sample is smeared for inspection, and the efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism detection, and in particular to a microorganism detection device and a microorganism detection method. Background Art

[0002] In the fields of food, medicine, etc., in order to ensure quality and safety, microbial inspections of products and raw materials are carried out, such as Staphylococcus aureus and its enterotoxins, Clostridium botulinum, Salmonella typhimurium, Vibrio parahaemolyticus, Listeria monocytogenes, Shigella, etc. in food. The detection method is to take samples from food, apply the samples to the culture medium (dish) after treating them with reagents, and then put the culture medium into the incubator for cooling to obtain colonies, and transfer the colonies to the detector to complete the inspection. It can be seen that the culture medium before detection is the core component for preparing colonies. However, since the culture medium is stored in a container, the culture medium needs to be taken out before use, and then manually disinfected, which is inefficient. Summary of the invention

[0003] In order to solve the above problems, the present invention provides the following technical solutions:

[0004] A microorganism detection device comprises an outer cylinder, an inner cylinder is fixed in the outer cylinder, a lifting frame is placed in the inner cylinder, a plurality of culture dishes consisting of a culture seat and a culture cover are placed on the lifting frame, the culture cover is buckled on the culture seat, a counterweight assembly is provided at the bottom of the lifting frame, when the lifting frame is placed in the inner cylinder with the culture dishes, the lifting frame is affected by the load of the culture dishes, so that the lifting frame descends relative to the counterweight assembly, and the culture cover is triggered to open upward by the counterweight assembly, an ultraviolet sterilization lamp is installed in the outer cylinder, a sterilization hole communicating with the inside and outside of the ultraviolet sterilization lamp is opened on the inner cylinder, a trigger assembly is provided between the bottom of the inner cylinder and the bottom of the lifting frame, when the lifting frame descends, the ultraviolet sterilization lamp is automatically energized by the trigger assembly, and the culture dishes are fully sterilized through the sterilization hole.

[0005] As a further preferred embodiment, a loading seat for loading culture dishes is provided at the bottom end of the lifting frame, a circle of assembly holes is provided in a circular array on the loading seat, the counterweight assembly includes a counterweight seat located below the loading seat, a plurality of guide rods fixed on the counterweight seat in a circular array, a spring mounted on each of the guide rods, and a warping portion arranged on the top end of the first guide rod, the guide rod is vertically upward, the warping portion extends from the periphery of the culture seat to the bottom of the culture cover, a limiting sleeve is fixed on the guide rod, the limiting sleeve is located above the loading seat, and the upper and lower ends of the spring are elastically supported between the loading seat and the counterweight seat.

[0006] As a further preferred embodiment, the trigger assembly includes a normally open button fixed in the middle of the bottom of the inner cylinder cavity, and a trigger rod fixed on the bottom surface of the loading seat, the trigger rod is vertically above the normally open button, and the normally open button electrically controls the ultraviolet sterilization lamp.

[0007] As a further preferred embodiment, a lifting rod is welded to one side of the bottom of the lifting frame, the lifting rod vertically reaches above the top of the inner cylinder and is provided with a lifting portion bent above the top of the inner cylinder, a storage space is formed between the lifting portion and the loading seat, a plurality of culture dishes are arranged on the loading seat from top to bottom, and the loading seat is a hollow structure.

[0008] As a further preference, an arc plate is fixed on the lifting rod, the arc plate extends from the bottom end to the top end along the height direction of the lifting rod, the arc bending direction of the arc plate faces the culture dish, and the arc plate is a hollow structure.

[0009] As further preferred, a cylinder cover is provided at the top end of the outer cylinder, and when the cylinder cover is covered on the outer cylinder, the lifting part is covered inside the inner cylinder.

[0010] As a further preferred embodiment, a sandwich wall cavity is formed between the outer cylinder and the inner cylinder, the ultraviolet sterilization lamp is located in the sandwich wall cavity, the ultraviolet sterilization lamp is a vertically upward spiral lamp, the sterilization hole is a vertically upward spiral groove, and the external-ray sterilization lamp corresponds to the sandwich wall cavity outside the spiral groove-shaped sterilization hole in a spiral manner.

[0011] The present invention also provides a microorganism detection method applicable to the above-mentioned microorganism detection device, comprising the following steps:

[0012] Step S01, wiping the workbench with disinfectant alcohol and transferring the sample to the workbench;

[0013] Step S02, sterilizing the culture dish by placing the culture dish in the inner cylinder and irradiating it with ultraviolet light;

[0014] Step S03, opening the cultivation cover of the culture dish, taking a sample from the sample through a dropper, and injecting the sample into the cultivation seat of the culture dish, putting the cultivation cover back on the cultivation seat to seal the sample, and shaking the culture dish to evenly distribute the sample in the cultivation seat;

[0015] Step S04, transferring the culture dish into an incubator, and obtaining microbial colonies after cooling down;

[0016] Step S05: Take out the culture dish, obtain the microbial colony sample, and transfer the microbial colony sample to a detection instrument to complete the detection.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] An outer cylinder and an inner cylinder are provided, an ultraviolet sterilization lamp is provided in the clamping cavity between the inner cylinder and the outer cylinder, and a culture dish is placed in the inner cylinder in a lifting frame carrying manner. When not testing, the cylinder cover is covered on the outer cylinder, and pressure is provided to the lifting part at the top of the lifting frame by the bottom surface of the cylinder cover. Firstly, the lifting frame will not swing freely in the inner cylinder, and secondly, the lifting frame is forced to descend along the inner cylinder under the action of all the culture dishes. The cultivation cover on the culture dish at the bottom falls on the warping part, and with the descending action, the cultivation cover is opened, that is, when these culture dishes are located in the inner cylinder with the lifting frame, the culture dish at the bottom is in an open state, and the loading seat will also descend with the trigger rod, so that the trigger rod is pressed against the normally open button, and the contacts of the normally open button are closed, and the ultraviolet sterilization lamp is automatically energized, and ultraviolet rays enter the inner cylinder through the sterilization hole. On the one hand, the light disinfects the outside of all the culture dishes, and on the other hand, the light is emitted into the culture dish at the bottom to complete the disinfection of the inside of the culture dish at the bottom. The cylinder cover is opened, and the lifting part is held to lift the lifting frame and all the culture dishes on the lifting frame outward together, and the culture dish at the bottom is removed for sample smearing. In addition, all other culture dishes will fall down in turn. At this time, the second culture dish at the bottom will fall on the loading seat as the bottommost culture dish, and the lifting frame will be reinstalled into the inner cylinder, and the cylinder cover will be closed. The cylinder cover will press the lifting part again, and the lifting part will push the lifting frame to drop again. The lifting frame will drop again with the loading seat relative to the counterweight seat, and the trigger rod will be pressed toward the normally open button again. The ultraviolet sterilization lamp will sterilize all the culture dishes again, and the bottommost culture dish will be opened by the warping part again to complete the internal sterilization. By analogy, when several culture dishes are used, the above process is repeated to take several culture dishes. Each time the lifting frame is taken and reinstalled, the second culture dish at the bottom will be opened as the bottommost culture dish, which not only completes the internal sterilization, but also the outside is sterilized like all other culture dishes, so that the culture dishes used for inspection can be sterilized as needed before smearing the sample for inspection, thereby improving efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of an embodiment of the present invention from a bottom-up perspective;

[0020] Figure 2 It is a schematic diagram of a cutaway embodiment of the present invention;

[0021] Figure 3 This is a schematic diagram of the placement position and disinfection principle of the culture dish after being cut open in accordance with an embodiment of the present invention;

[0022] Figure 4 For the implementation mode of the present invention, Figure 3 The main view plane diagram is derived;

[0023] Figure 5 It is a schematic diagram of only the lifting frame in an embodiment of the present invention when viewed from the bottom;

[0024] Figure 6 This is a schematic diagram of an embodiment of the present invention when only a culture dish is placed on the lifting rack;

[0025] Figure 7 For the implementation mode of the present invention, Figure 6 A schematic diagram from another perspective.

[0026] In the figure: 1. outer cylinder; 2. inner cylinder; 3. lifting frame; 41. cultivation seat; 42. cultivation cover; 4. culture dish; 5. counterweight assembly; 6. ultraviolet sterilization lamp; 7. sterilization hole; 8. trigger assembly; 9. loading seat; 10. assembly hole; 11. counterweight seat; 12. guide rod; 13. spring; 14. warping part; 15. normally open button; 16. trigger rod; 17. lifting rod; 18. pulling part; 19. storage space; 20. clamping wall cavity; 21. limit sleeve; 22. arc plate; 23. cylinder cover. DETAILED DESCRIPTION

[0027] The above and other embodiments and advantages of the present invention are described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments.

[0028] In one embodiment, if Figure 1-Figure 7 As shown: This embodiment provides a microorganism detection device, including an outer cylinder 1, an inner cylinder 2 is fixed in the outer cylinder 1, a lifting frame 3 is placed in the inner cylinder 2, a plurality of culture dishes 4 consisting of a culture seat 41 and a culture cover 42 are placed on the lifting frame 3, the culture cover 42 is buckled on the culture seat 41, and a counterweight component 5 is provided at the bottom of the lifting frame 3. When the lifting frame 3 is placed in the inner cylinder 2 with the culture dishes 4, the lifting frame 3 is affected by the load of the culture dishes 4, so that the lifting frame 3 descends relative to the counterweight component 5, and the culture cover 42 is triggered to open upward by the counterweight component 5. An ultraviolet sterilization lamp 6 is installed in the outer cylinder 1, and a sterilization hole 7 connected to the ultraviolet sterilization lamp 6 inside and outside is opened on the inner cylinder 2. A trigger component 8 is provided between the bottom of the inner cylinder 2 and the bottom of the lifting frame 3. When the lifting frame 3 descends, the ultraviolet sterilization lamp 6 is automatically energized by the trigger component 8, and the culture dishes 4 are fully sterilized through the sterilization hole 7.

[0029] As a further preferred embodiment, a loading seat 9 for loading the culture dish 4 is provided at the bottom end of the lifting frame 3, and a circle of assembly holes 10 are opened in a circular array on the loading seat 9. The counterweight assembly 5 includes a counterweight seat 11 located below the loading seat 9, including a plurality of guide rods 12 fixed on the counterweight seat 11 in a circular array, including a spring 13 sleeved on each guide rod 12, and also including a warping portion 14 arranged on the top end of the first guide rod 12, the guide rod 12 is vertically upward, and the warping portion 14 extends from the periphery of the culture seat 41 to the bottom of the culture cover 42, a limiting sleeve 21 is fixed on the guide rod 12, and the limiting sleeve 21 is located above the loading seat 9, and the upper and lower ends of the spring 13 are elastically supported between the loading seat 9 and the counterweight seat 11, and the trigger assembly 8 includes a normally open button 15 fixed in the middle of the bottom of the inner cylinder 2, including a trigger rod 16 fixed on the bottom surface of the loading seat 9, the trigger rod 16 is vertically above the normally open button 15, and the normally open button 15 electrically controls the ultraviolet sterilization lamp 6.

[0030] like Figure 2 As shown, a lifting rod 17 is welded to one side of the bottom of the lifting frame 3. The lifting rod 17 vertically reaches the top of the inner cylinder 2 and is provided with a lifting portion 18 bent above the top of the inner cylinder 2. A storage space 19 is formed between the lifting portion 18 and the loading seat 9. Multiple culture dishes 4 are arranged on the loading seat 9 from top to bottom according to the storage space 19. The loading seat 9 is a hollow structure and is lightweight.

[0031] like Figures 1 to 3 As shown, a cylinder cover 23 is provided at the top end of the outer cylinder 1. When the cylinder cover 23 is covered on the outer cylinder 1, the lifting part 18 is covered inside the inner cylinder 2. The cylinder cover 23 is connected to the outer cylinder 1 by a threaded connection. The greater the degree of rotation of the cylinder cover 23 on the inner cylinder 2, the higher the degree of compression of the inner surface on the lifting part 18, the greater the descent range of the lifting part 18 with the lifting rod 17, and the lifting rod 17 with the entire lifting frame 3, the closer the loading seat 9 is to the counterweight seat 11, and the greater the opening degree of the culture cover 42 on the culture dish 4 at the bottom relative to the warping part 14.

[0032] like Figure 2 As shown, a sandwich cavity 20 is formed between the outer cylinder 1 and the inner cylinder 2, and the ultraviolet sterilization lamp 6 is located in the sandwich cavity 20. The ultraviolet sterilization lamp 6 is a vertically upward spiral lamp, and the sterilization hole 7 is a vertically upward spiral groove. The ultraviolet sterilization lamp 6 corresponds to the sandwich cavity 20 outside the spiral groove-shaped sterilization hole 7 in a spiral manner. The ultraviolet sterilization lamp 6 is confined in the sandwich cavity 20 and will not directly contact the culture dish 4. When the culture dish 4 is placed in the inner cylinder 2, the outer edge of the culture dish 4 is at the inner side of the cavity wall of the inner cylinder 2. The light emitted by the ultraviolet sterilization lamp 6 only needs to pass through the sterilization hole 7 and then act on the culture dish 4 to complete the sterilization of the outside and inside of the culture dish 4.

[0033] Principle and technical effect: When not testing, all the culture dishes 4 are stacked together from top to bottom and placed on the lifting frame 3. The culture dish 4 at the bottom is placed on the loading seat 9 as a support, and the bottom surface of the culture cover 42 of the culture dish 4 at the bottom just falls on the warped portion 14. Then, the lifting frame 3 carries all the culture dishes 4 into the inner cylinder 2, and the counterweight seat 11 falls on the bottom of the inner cylinder 2. The cylinder cover 23 is covered on the outer cylinder 1, and the cylinder cover 23 is screwed on the outer cylinder 1. As the cylinder cover 23 descends when being screwed, the bottom surface of the cylinder cover 23 provides pressure to the lifting portion 18, forcing the lifting frame 3 to descend along the inner cylinder 2. At this time, the loading seat 9 descends relative to the counterweight seat 11. When the loading seat 9 descends, the guide rod 12 plays a role of steady guidance. While descending, the spring 13 is compressed and shortened. Since the culture cover 42 on the culture dish 4 at the bottom falls on the warping portion 14, and the warping portion 14 is the top structure of the guide rod 12, and the guide rod 12 does not move, the bottom When the loading seat 9 is lowered, the trigger rod 16 will also be lowered, so that the trigger rod 16 is pressed against the normally open button 15. The contact of the normally open button 15 is closed, and the ultraviolet sterilization lamp 6 is automatically energized. The ultraviolet rays enter the inner cylinder 2 through the sterilization hole 7. On the one hand, the light rays disinfect the outside of all the culture dishes 4, and on the other hand, the light rays are injected into the culture dish 4 at the bottom to complete the disinfection of the inside of the culture dish 4 at the bottom. The cylinder cover 23 is opened, and the lifting part 18 is held to lift the lifting frame 3 and all the culture dishes 4 on the lifting frame 3 outward together. The pressure on the loading seat 9 disappears, the spring 13 rebounds and becomes longer, and the guide rod 12 will not fall off under the restriction of the limit sleeve 21, and it will also be lifted together with the counterweight seat 11 along with the lifting frame 3, and the culture dish 4 at the bottom is removed for sample smearing.In addition, all other culture dishes 4 will fall down in sequence. At this time, the second culture dish 4 at the bottom falls on the loading seat 9 as the bottommost culture dish 4. The lifting frame 3 is reinstalled into the inner cylinder 2, and the cylinder cover 23 is closed. The cylinder cover 23 presses the lifting part 18 again, and the lifting part 18 pushes the lifting frame 3 to descend again. The lifting frame 3 and the loading seat 9 are lowered again relative to the counterweight seat 11. The trigger rod 16 presses the normally open button 15 again, and the ultraviolet sterilization lamp 6 sterilizes all the culture dishes 4 again. The bottommost culture dish 4 is opened by the warping part 14 again, and the process is completed. Internal sterilization, and by analogy, several culture dishes 4 are used, and the above process is repeated to take several culture dishes 4. Each time the lifting rack 3 is taken out and reinstalled, the second culture dish 4 at the bottom will be opened as the bottommost culture dish 4, not only completing the internal sterilization, but also like all other culture dishes 4, when the lifting rack 3 is stored in the inner cylinder 2, the outside can be sterilized multiple times, so that the culture dishes 4 used for inspection can be sterilized as they are used before smearing the sample for inspection, saving the step of sterilizing the culture dishes 4 separately, and improving the efficiency of transferring to the detector for inspection.

[0034] like Figure 2 , Figure 3 , Figure 5 as well as Figure 7 As shown, an arc plate 22 is fixed on the lifting rod 17, and the arc plate 22 extends from the bottom end to the top end along the height direction of the lifting rod 17. The arc bending direction of the arc plate 22 faces the culture dish 4, and the arc plate 22 is a hollow structure. When multiple culture dishes 4 are placed on the loading seat 9, their edges will lean against the arc plate 22 and be arranged in sequence along the height direction of the arc plate 22. The arc surface of the arc plate 22 is used to form a certain positioning for the culture dishes 4, so as to prevent them from falling from the loading seat 9 when they are used.

[0035] It needs to be further explained that: during the actual assembly, a switch will be set on the outer cylinder 1, and the switch is controlled between the normally open button 15 and the ultraviolet sterilization lamp 6. When the switch is turned off when not in use, the normally open button 15 can be disabled. Even if a culture dish 4 is placed in the inner cylinder 2 with the lifting frame 3, even if the cylinder cover 23 is closed above the outer cylinder 1, the lifting frame 3 is squeezed and pulled by the inner surface. The lifting seat 9 is lowered, and the trigger rod 16 is pressed on the normally open button 15 by the descending action of the loading seat 9. The normally open button 15 is also in a power-off state, and the ultraviolet sterilization lamp 6 will not work. The ultraviolet sterilization lamp 6 can be powered by a battery or an external plug. The existing power supply technology is not described in detail in the present invention.

[0036] The present invention also provides a microorganism detection method applicable to the above-mentioned microorganism detection device, comprising the following steps:

[0037] Step S01, wiping the workbench with disinfectant alcohol and transferring the sample to the workbench;

[0038] Step S02, placing the culture dish 4 in the inner cylinder 2 and sterilizing the culture dish 4 in advance by ultraviolet irradiation;

[0039] Step S02, open the culture cover 42 of the culture dish 4, take a sample from the sample through a dropper, and inject the sample into the culture seat 41 of the culture dish 4, put the culture cover 42 back on the culture seat 41 to seal the sample, and shake the culture dish 4 to evenly distribute the sample in the culture seat 41;

[0040] Step S04, transfer the culture dish 4 into the incubator, and obtain microbial colonies after cooling down;

[0041] Step S05, taking out the culture dish 4, obtaining a microbial colony sample, and transferring the microbial colony sample to a detection instrument to complete detection.

[0042] The above orientation designations do not represent the specific orientations of the components in this implementation scheme. This implementation scheme is only for the convenience of describing the scheme, and the orientations are described relatively with reference to the figures. In essence, the specific orientations of the components are described according to their actual installation and actual use as well as the customary orientations of technicians in this field. This is hereby explained.

[0043] The specific implementation methods described above further describe the invention purpose, technical solutions, and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the protection scope of the present invention. It is particularly pointed out that for those skilled in the art, any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A microorganism detection device, characterized in that: The invention comprises an outer cylinder (1), an inner cylinder (2) is fixed inside the outer cylinder (1), a lifting frame (3) is placed inside the inner cylinder (2), a plurality of culture dishes (4) consisting of a culture seat (41) and a culture cover (42) are placed on the lifting frame (3), the culture cover (42) is buckled on the culture seat (41), a counterweight component (5) is provided at the bottom of the lifting frame (3), and when the lifting frame (3) is placed in the inner cylinder (2) with the culture dishes (4), the lifting frame (3) is affected by the weight of the culture dishes (4), so that the lifting frame (3) is relatively stable relative to the counterweight component The outer cylinder (1) is provided with an ultraviolet sterilizing lamp (6), and the inner cylinder (2) is provided with a sterilizing hole (7) which is in communication with the ultraviolet sterilizing lamp (6) inside and outside. A triggering assembly (8) is provided between the bottom of the inner cylinder (2) and the bottom of the lifting frame (3). When the lifting frame (3) is lowered, the ultraviolet sterilizing lamp (6) is automatically powered on by the triggering assembly (8), and the culture dish (4) is fully sterilized through the sterilizing hole (7).

2. The microorganism detection device according to claim 1, characterized in that: The bottom end of the lifting frame (3) is provided with a loading seat (9) for loading the culture dish (4), and a circle of assembly holes (10) are provided in an annular array on the loading seat (9). The counterweight assembly (5) comprises a counterweight seat (11) located below the loading seat (9), a plurality of guide rods (12) fixed on the counterweight seat (11) in an annular array, a spring (13) sleeved on each of the guide rods (12), and a warping portion (14) arranged on the top end of the first guide rod (12), wherein the guide rod (12) is vertically upward, and the warping portion (14) extends from the periphery of the culture seat (41) to the bottom of the culture cover (42), and a limiting sleeve (21) is fixed on the guide rod (12), and the limiting sleeve (21) is located above the loading seat (9), and the upper and lower ends of the spring (13) are elastically supported between the loading seat (9) and the counterweight seat (11).

3. The microorganism detection device according to claim 1, characterized in that: The trigger assembly (8) comprises a normally open button (15) fixed to the middle of the bottom of the inner cylinder (2), and a trigger rod (16) fixed to the bottom surface of the loading seat (9), wherein the trigger rod (16) is vertically above the normally open button (15), and the normally open button (15) electrically controls the ultraviolet germicidal lamp (6).

4. The microorganism detection device according to claim 1, characterized in that: A lifting rod (17) is welded to one side of the bottom of the lifting frame (3), and the lifting rod (17) vertically reaches the top of the inner cylinder (2) and is provided with a lifting portion (18) bent above the top of the inner cylinder (2), and a storage space (19) is formed between the lifting portion (18) and the loading seat (9), and a plurality of culture dishes (4) are arranged from top to bottom on the loading seat (9), and the loading seat (9) is a hollow structure.

5. The microorganism detection device according to claim 1, characterized in that: An arc-shaped plate (22) is fixed on the lifting rod (17), and the arc-shaped plate (22) extends from the bottom end to the top end along the height direction of the lifting rod (17). The arc-shaped bending direction of the arc-shaped plate (22) faces the culture dish (4), and the arc-shaped plate (22) is a hollow structure.

6. The microorganism detection device according to claim 2, characterized in that: A cylinder cover (23) is provided at the top end of the outer cylinder (1). When the cylinder cover (23) is covered on the outer cylinder (1), the lifting portion (18) is covered inside the inner cylinder (2).

7. The microorganism detection device according to claim 3, characterized in that: A sandwich wall cavity (20) is formed between the outer cylinder (1) and the inner cylinder (2), the ultraviolet sterilization lamp (6) is located in the sandwich wall cavity (20), the ultraviolet sterilization lamp (6) is a vertically upward spiral lamp, the sterilization hole (7) is a vertically upward spiral groove, and the ultraviolet sterilization lamp (6) corresponds to the sandwich wall cavity (20) outside the spiral groove-shaped sterilization hole (7) in a spiral manner.

8. A microorganism detection method applicable to the microorganism detection device according to claim 2, characterized in that: The following steps are involved: Step S01, wiping the workbench with disinfectant alcohol and transferring the sample to the workbench; Step S02, placing the culture dish (4) in the inner cylinder (2) and sterilizing the culture dish (4) by ultraviolet irradiation; Step S02, opening the culture cover (42) of the culture dish (4), taking a sample from the sample through a dropper, and injecting the sample into the culture seat (41) of the culture dish (4), putting the culture cover (42) back on the culture seat (41) to seal the sample, and shaking the culture dish (4) to evenly distribute the sample in the culture seat (41); Step S04, transferring the culture dish (4) into an incubator, and obtaining a microbial colony after cooling down; Step S05, taking out the culture dish (4), obtaining a microbial colony sample, and transferring the microbial colony sample to a detection instrument to complete the detection.

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