Hair drug detection reagent and detection device

Abstract

The invention provides a hair drug detection reagent and a detection device, and belongs to the technical field of drug detection, the reagent comprises a lysis solution and a specific antibody labeling detection reagent; the lysate comprises the following components: a surfactant, a chelating agent, a reducing agent, an enzyme preparation, a stabilizer, a preservative and a buffer solution. The device comprises the reagent. The components of the lysate have a synergistic effect, so that a hair sample can be quickly and effectively lysed, and drugs or metabolites thereof can be released.

Description

Technical Field

[0001] The present invention relates to the technical field of drug detection, and in particular to a hair drug detection reagent and a detection device. Background Art

[0002] At present, for the rapid screening of drug addicts, biological test samples mainly include urine, saliva and hair. Compared with urine and saliva, hair samples have the advantages of stable properties, convenient collection, easy storage, detection time limit, wide application range, and less chance of contamination. However, traditional hair drug detection technology has problems such as long detection time, low sensitivity, and cumbersome operation. Therefore, it is of great significance to develop a hair drug detection reagent that is efficient, sensitive, and easy to operate. To this end, the present invention provides a hair drug detection reagent and a detection device. Summary of the invention

[0003] The purpose of the present invention is to provide a hair drug detection reagent and a detection device.

[0004] To solve the above technical problems, the purpose of the present invention is achieved as follows:

[0005] A hair drug detection reagent, comprising a lysis solution and a specific antibody-labeled detection reagent;

[0006] The lysate comprises the following components by mass percentage: 5%-10% of a surfactant, 0.1%-2% of a chelating agent, 0.5%-5% of a reducing agent, 0.01%-1% of an enzyme preparation, 5%-10% of a stabilizer, 0.05%-0.2% of a preservative, and 80%-90% of a buffer solution.

[0007] On the basis of the above scheme and as a preferred scheme of the above scheme, the surfactant is any one of sodium lauryl sulfate or cocamidopropyl betaine or a combination of both.

[0008] On the basis of the above scheme and as a preferred scheme of the above scheme, the chelating agent is any one of ethylenediaminetetraacetic acid or citric acid or a combination of both.

[0009] On the basis of the above scheme and as a preferred scheme of the above scheme, the reducing agent is any one of dithiothreitol or β-mercaptoethanol or a combination of both.

[0010] On the basis of the above scheme and as a preferred scheme of the above scheme, the enzyme preparation is any one of keratinase, proteinase K and cathepsin, or a combination of at least two of them.

[0011] On the basis of the above scheme and as a preferred scheme of the above scheme, the stabilizer is any one of glycerol or trehalose or a combination of the two.

[0012] On the basis of the above scheme and as a preferred scheme of the above scheme, the preservative is any one of Proclin 300, sodium benzoate and sodium benzoate or a combination of at least two of them.

[0013] On the basis of the above scheme and as a preferred scheme of the above scheme, the buffer is any one of Tris-HCl buffer or phosphate buffer.

[0014] On the basis of the above scheme and as a preferred scheme of the above scheme, the pH value of the buffer solution is 7.1-7.6.

[0015] A hair drug detection device comprises the hair drug detection reagent mentioned above.

[0016] The beneficial effects of the present invention are:

[0017] 1. Efficient lysis: The components of the lysis solution work synergistically to quickly and effectively lyse hair samples and release drugs or their metabolites.

[0018] 2. High specificity: Through the specific degradation effect of enzyme preparations, the interference with non-target substances is reduced and the specificity of detection is improved.

[0019] 3. Protect hair structure: Minimize damage to hair structure during the lysis process to facilitate subsequent detection and analysis.

[0020] 4. Easy operation: The preparation method of the lysate is simple and easy to operate, suitable for large-scale preparation and application. DETAILED DESCRIPTION

[0021] The present invention is further described below in conjunction with specific embodiments.

[0022] Embodiment 1:

[0023] A hair drug detection reagent, comprising a lysis solution and a specific antibody-labeled detection reagent;

[0024] The lysate comprises the following components by mass percentage: 5%-10% of a surfactant, 0.1%-2% of a chelating agent, 0.5%-5% of a reducing agent, 0.01%-1% of an enzyme preparation, 5%-10% of a stabilizer, 0.05%-0.2% of a preservative, and 80%-90% of a buffer solution.

[0025] The specific labeled detection reagent is prepared by screening highly specific antibodies obtained by immunizing mice or other animals, and labeling them with biotin or other markers to prepare a specific antibody-labeled detection reagent.

[0026] Among them, the surfactant is sodium dodecyl sulfate, with a mass percentage of 5%; the chelating agent is ethylenediaminetetraacetic acid, with a mass percentage of 0.1%; the reducing agent is dithiothreitol, with a mass percentage of 0.5%; the enzyme preparation is keratinase, with a mass percentage of 0.01%; the stabilizer is glycerol, with a mass percentage of 5%; the preservative is Proclin300, with a mass percentage of 0.05%; the buffer is Tris-HCl buffer, with a mass percentage of 90% and a pH value of 7.1-7.6.

[0027] The above ingredients are mixed in proportion, dissolved and mixed under appropriate conditions until a uniform and transparent lysate is formed. The lysate is filtered and sterilized to ensure that it is sterile and free of impurities, and then packaged and stored.

[0028] A hair drug detection device comprises the above-mentioned hair drug detection reagent.

[0029] At the same time, the hair drug detection device also includes components such as sample addition holes, reaction areas, and observation windows, which are used to carry lysis solutions, detection reagents, and hair samples, and to perform reactions and detections.

[0030] When in use, the hair sample is cut into small pieces of appropriate length, added to the lysis solution, and left to stand at a suitable temperature for a period of time to release the drugs or their metabolites from the hair. The treated hair sample is mixed with a detection reagent labeled with a specific antibody, and reacted under suitable conditions to form an antigen-antibody complex. The reaction results are observed by fluorescent immunochromatography, colloidal gold immunochromatography or other detection methods to determine whether the hair sample contains drugs or their metabolites.

[0031] Embodiment 2:

[0032] A hair drug detection reagent, comprising a lysis solution and a specific antibody-labeled detection reagent;

[0033] The lysate comprises the following components by mass percentage: 5%-10% of a surfactant, 0.1%-2% of a chelating agent, 0.5%-5% of a reducing agent, 0.01%-1% of an enzyme preparation, 5%-10% of a stabilizer, 0.05%-0.2% of a preservative, and 80%-90% of a buffer solution.

[0034] The specific labeled detection reagent is prepared by screening highly specific antibodies obtained by immunizing mice or other animals, and labeling them with biotin or other markers to prepare a specific antibody-labeled detection reagent.

[0035] Among them, the surfactant is cocamidopropyl betaine, with a mass percentage of 10%; the chelating agent is citric acid, with a mass percentage of 2%; the reducing agent is β-mercaptoethanol, with a mass percentage of 5%; the enzyme preparation is proteinase K, with a mass percentage of 1%; the stabilizer is trehalose, with a mass percentage of 10%; the preservative is sodium benzoate, with a mass percentage of 0.2%; the buffer is phosphate buffer, with a mass percentage of 80% and a pH value of 7.1-7.6.

[0036] A hair drug detection device comprises the above-mentioned hair drug detection reagent.

[0037] Embodiment three:

[0038] A hair drug detection reagent, comprising a lysis solution and a specific antibody-labeled detection reagent;

[0039] The lysate comprises the following components by mass percentage: 5%-10% of a surfactant, 0.1%-2% of a chelating agent, 0.5%-5% of a reducing agent, 0.01%-1% of an enzyme preparation, 5%-10% of a stabilizer, 0.05%-0.2% of a preservative, and 80%-90% of a buffer solution.

[0040] The specific labeled detection reagent is prepared by screening highly specific antibodies obtained by immunizing mice or other animals, and labeling them with biotin or other markers to prepare a specific antibody-labeled detection reagent.

[0041] Among them, the combination of the surfactant sodium lauryl sulfate and cocamidopropyl betaine has a mass percentage of 7%; the chelating agent is a combination of ethylenediaminetetraethyl and or citric acid, with a mass percentage of 1%; the reducing agent is a combination of dithiothreitol and β-mercaptoethanol, with a mass percentage of 2%; the enzyme preparation cathepsin has a mass percentage of 0.5%; the stabilizer is a combination of glycerol and trehalose, with a mass percentage of 7%; the preservative is a combination of sodium benzoate and sodium pyrophosphate, with a mass percentage of 0.1%; the buffer is a phosphate buffer, with a mass percentage of 85% and a pH value of 7.1-7.6.

[0042] A hair drug detection device comprises the above-mentioned hair drug detection reagent.

[0043] The preferred specific embodiments of the present invention are described in detail above. It should be understood that a person skilled in the art can make many modifications and changes based on the concept of the present invention without creative work. Therefore, any technical solution that can be obtained by a person skilled in the art through logical analysis, reasoning or limited experiments based on the concept of the present invention on the basis of the prior art should be within the scope of protection determined by the claims.

Claims

1. A hair drug detection reagent, characterized in that: Including lysis buffer and specific antibody labeling detection reagents; The lysate comprises the following components by mass percentage: 5%-10% of a surfactant, 0.1%-2% of a chelating agent, 0.5%-5% of a reducing agent, 0.01%-1% of an enzyme preparation, 5%-10% of a stabilizer, 0.05%-0.2% of a preservative, and 80%-90% of a buffer solution.

2. A hair drug detection reagent according to claim 1, characterized in that: The surfactant is any one of sodium lauryl sulfate or cocamidopropyl betaine or a combination of both.

3. A hair drug detection reagent according to claim 1, characterized in that: The chelating agent is any one of ethylenediaminetetraacetic acid or citric acid or a combination of the two.

4. A hair drug detection reagent according to claim 1, characterized in that: The reducing agent is any one of dithiothreitol and β-mercaptoethanol or a combination of the two.

5. A hair drug detection reagent according to claim 1, characterized in that: The enzyme preparation is any one of keratinase, proteinase K and cathepsin, or a combination of at least two of them.

6. A hair drug detection reagent according to claim 1, characterized in that: The stabilizer is any one of glycerol or trehalose or a combination of the two.

7. A hair drug detection reagent according to claim 1, characterized in that: The preservative is any one of Proclin 300, sodium benzoate and sodium benzoate, or a combination of at least two of them.

8. A hair drug detection reagent according to claim 1, characterized in that: The buffer is any one of Tris-HCl buffer or phosphate buffer.

9. A hair drug detection reagent according to claim 1 or 7, characterized in that: The pH value of the buffer solution is 7.1-7.

6.

10. A hair drug detection device, characterized in that: The detection device comprises the hair drug detection reagent according to any one of claims 1-9.