Application of sericin solution in improving plant salt tolerance and sericin plant foliar spraying agent

Abstract

The application discloses application of a silk fibroin liquid in improving plant salt tolerance and a silk fibroin plant foliar spraying agent, and relates to the technical field of pesticide production. The application can be used for improving plant salt tolerance, developing the application of silk fibroin in agriculture, especially in the application of soil salinization, and not only has the possibility of providing new ideas and methods for solving the problem of soil salinization, but also has the possibility of opening up a new field for the application of silk fibroin and bringing new opportunities and challenges to agricultural production.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pesticide production, more particularly to application of silk sericin liquid in improving plant salt tolerance and silk sericin plant foliar spraying agent. BACKGROUND

[0002] Soil salinization has become one of the important stresses in agricultural production. There are various reasons for soil salinization, including less rainfall, weathering of primary rocks, improper irrigation and fertilization during cultivation, etc. High-salt soil leads to ion toxicity, osmotic stress and oxidative stress, making it difficult for roots to absorb water, disrupting normal physiological metabolism of plants, and thus hindering the growth and development of plants, seriously affecting crop yield in agricultural production. How to improve plant salt tolerance and cultivate salt-tolerant new varieties has become an important research goal and an urgent problem to be solved for the development and utilization of saline land.

[0003] Silk is a natural high-molecular protein fiber and an important textile material. It does not require a large amount of chemical substances in the production process, and thus is more environmentally friendly. In addition, the biodegradability of silk protein means that it can be rapidly decomposed in soil, reducing the impact on the environment. With in-depth study of the properties of silk, the application value of silk has been expanded in multiple fields. Silk protein mainly includes two components, namely silk sericin and silk fibroin. Silk sericin and silk fibroin are used in the fields of cosmetics, food and biomedical materials due to their excellent biocompatibility, morphological plasticity, moisturizing and antioxidant functions. Among them, silk sericin has been developed and utilized in cosmetic production due to its good moisturizing function, and it is necessary to develop the application of silk sericin in agriculture. SUMMARY

[0004] To solve the above problems, the present application provides application of silk sericin liquid in improving plant salt tolerance and silk sericin plant foliar spraying agent to improve the salt tolerance of plants.

[0005] The present application is realized by the following technical solutions:

[0006] The present application provides, in a first aspect, application of silk sericin liquid in improving plant salt tolerance, wherein the concentration of the silk sericin liquid is 0.1 g / L to 1 g / L.

[0007] Preferably, the concentration of the silk sericin liquid is 0.2 g / L.

[0008] Preferably, the silk sericin liquid is sprayed on the front of the leaves, and the spraying amount per plant per time is 1 mL to 2 mL.

[0009] Preferably, the improvement of plant salt tolerance is manifested as an increase in plant height, stem diameter and biomass under salt stress environment.

[0010] Preferably, the salt stress environment refers to a salt stress environment with a sodium chloride concentration of 100 mM to 500 mM.

[0011] Preferably, the plant includes tomato.

[0012] Preferably, the preparation method of the sericin solution is to take a cocoon, boil in water to dissolve sericin, remove undissolved silk fibroin, and obtain a sericin solution.

[0013] The ratio of the clean cocoon to water is 5 g to 10 g: 200 mL.

[0014] The second aspect of the present application provides a sericin plant foliage spraying agent, which is the sericin solution.

[0015] Compared with the prior art, the present application has the following beneficial effects:

[0016] The present application prepares a sericin solution from silk protein, and the concentration of the sericin solution is 0.1 g / L to 1 g / L. Spraying the sericin solution on the front of the leaves can improve the salt tolerance of the plant and improve the growth of the plant under salt stress, which is manifested as the increase of plant height, stem diameter and biomass. Therefore, the sericin solution can be used as a new spraying agent for improving the salt tolerance of plants, which opens up the application of silk protein in agriculture, especially in the application of soil salinization. It not only provides a new way and method for solving the problem of soil salinization, but also opens up a new field for the application of silk protein, and brings new opportunities and challenges for energy-saving and environment-friendly agricultural production. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the following embodiment or prior art description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0018] Figure 1 The growth of plants sprayed with distilled water and sericin solution on the front of the leaves.

[0019] Among them, A is sprayed with distilled water on the front of the leaves, HS1, three pots are three parallel tests; B is sprayed with sericin solution on the front of the leaves, DS, three pots are three parallel tests.

[0020] Figure 2 The growth indexes of plants sprayed with sericin solution on the front of the leaves and plants sprayed with distilled water on the front of the leaves are measured and analyzed.

[0021] Wherein, A is the relative growth rate of plant height of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, B is the salt tolerance index of plant height of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, C is the relative growth rate of stem diameter of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, D is the salt tolerance index of stem diameter of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, E is the root fresh weight of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, F is the relative reduction rate of biomass calculated by root fresh weight of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, G is the root dry weight of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, H is the relative reduction rate of biomass calculated by root dry weight of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, I is the aboveground fresh weight of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, J is the relative reduction rate of biomass calculated by aboveground fresh weight of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, K is the aboveground dry weight of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front, L is the relative reduction rate of biomass calculated by aboveground dry weight of plant sprayed with silk fibroin liquid on leaf front and plant sprayed with distilled water on leaf front.

[0022] Figure 3 The plant growth conditions of spraying distilled water and silk fibroin liquid on leaf back.

[0023] Wherein, A is spraying distilled water on leaf back, HS2, three potted plants are three parallel tests; B is spraying silk fibroin liquid on leaf back, BS, three potted plants are three parallel tests.

[0024] Figure 4 The measurement and analysis of various growth indexes of plant sprayed with silk fibroin liquid on leaf back and plant sprayed with distilled water on leaf back.

[0025] Wherein, A is the relative growth rate of plant height of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, B is the salt tolerance index of plant height of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, C is the relative growth rate of stem diameter of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, D is the salt tolerance index of stem diameter of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, E is the fresh weight of roots of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, F is the relative reduction rate of biomass calculated by the fresh weight of roots of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, G is the dry weight of roots of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, H is the relative reduction rate of biomass calculated by the dry weight of roots of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, I is the fresh weight of the above-ground part of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, J is the relative reduction rate of biomass calculated by the fresh weight of the above-ground part of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, K is the dry weight of the above-ground part of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves, and L is the relative reduction rate of biomass calculated by the dry weight of the above-ground part of the plant sprayed with sericin liquid on the back of leaves and the plant sprayed with distilled water on the back of leaves. DETAILED DESCRIPTION

[0026] In order to facilitate the understanding of the present application, the present application will be described more fully below, and preferred embodiments of the present application will be given. However, the present application can be implemented in many different forms, and is not limited to the embodiments described herein. Rather, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terminology used in the description of the present application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application.

[0028] The inventive concept of the present application is as follows:

[0029] Sericin is a pure natural protein and has been applied in the fields of cosmetics and biological medicine. Considering that the solution or material prepared from sericin has good biocompatibility, moisturizing property, antioxidant effect, etc., it is expected that sericin solution used as an agricultural spraying agent can play a role in relieving the adaptability of plants to adverse environmental stress. However, there is no research on the use of sericin in improving the resistance of crops.

[0030] Based on this, the present application provides the use of sericin liquid in improving the salt tolerance of plants, and the concentration of the sericin liquid is 0.1 g / L to 1 g / L. This concentration range can ensure that the sericin solution can be effectively absorbed by plants and play a role after being sprayed on the leaves of plants, thereby improving the salt tolerance of plants.

[0031] Based on the same inventive concept, the present application provides a sericin plant foliar spray agent, which is the sericin solution. The spray agent can improve the salt tolerance of plants and improve the growth of plants under salt stress, specifically in the form of increased plant height, stem diameter and biomass. Through the implementation of the present application, sericin not only has applications in the fields of cosmetics and biomedicine, but also has a new use as an agricultural spray agent ingredient, providing a new spray agent ingredient for the development of agricultural spray agents.

[0032] In order to better illustrate the present application, the following embodiments are further described in conjunction with the present application. The following are specific embodiments.

[0033] Example 1

[0034] The preparation method of the sericin solution comprises: taking a cocoon, removing the outermost and innermost silk fibers, and taking the middle clean part as a clean cocoon for sericin solution preparation. 5g of clean cocoon is cut into pieces and boiled in 200mL of distilled water for 10 minutes; the remaining undissolved silk is removed, and the obtained solution is a sericin solution.

[0035] Example 2

[0036] The preparation method of the sericin solution comprises: taking a cocoon, removing the outermost and innermost silk fibers, and taking the middle clean part as a clean cocoon for sericin solution preparation. 10g of clean cocoon is cut into pieces and boiled in 200mL of distilled water for 10 minutes; the remaining undissolved silk is removed, and the obtained solution is a sericin solution.

[0037] Experiment 1

[0038] The experiment verifies the ability of sericin solution to improve the salt tolerance of plants when sprayed on the front of the leaves.

[0039] (I) Preparation of experimental materials

[0040] The obtained sericin solution is detected for concentration by BCA method, and diluted with distilled water to 0.2g / L for subsequent experiments.

[0041] The plant test material is tomato, and the variety is Ailsa craig. The tomato seeds are first sterilized with 70% alcohol for 30s, 10% sodium hypochlorite for 10 minutes, washed with sterile water for 5 times, dried with sterile filter paper, and then placed in a 28℃ culture in the dark until germination. The germinated tomato seeds are transplanted in pots and placed in a light incubator. The light is divided into two stages: stage one is light 22000xl, 25℃, 16h, and stage two is dark without light, 18℃, 8h culture. When the tomato grows to the three-leaf-one-heart stage, the experiment begins.

[0042] (ii) Experimental design

[0043] Tomato plants with the same growth potential and size were used for the experiment. Two groups of experimental plants were set up, at least 10 plants in each group: group A was the distilled water group, the leaves were sprayed with distilled water on the front, marked as HS1; group B was the sericin group, the leaves were sprayed with sericin solution on the front, marked as DS.

[0044] The spraying step was as follows: distilled water or sericin solution was placed in a 200 mL spray bottle, and the leaves were sprayed on the front at the time point of light culture box in darkness to light, 1.5 mL each time, to ensure uniform spraying of the leaves.

[0045] The plant height and stem diameter data of the tested plants were recorded before treatment, they were irrigated with 350 mM NaCl solution every 3 days, continuously treated for 18 days, and photographed and recorded during the spraying experiment. After the treatment, the plant height and stem diameter data were recorded, the roots and aboveground parts were separated, and the fresh weight was measured, and the dry weight was measured after 5 days of 85°C drying treatment.

[0046] In addition, two groups of experimental plants were set up as controls, without salt treatment, and the other treatment and observation steps were the same as groups A and B, as a reference for calculating the salt tolerance index and relative decrease in biomass.

[0047] The experimental data obtained were calculated according to the following formula:

[0048] Relative growth rate (RGR) = (Y t2 -Y t1 ) / (t2-t1).

[0049] Salt tolerance index (STI) = (Y 盐t2 -Y 盐t1 ) / (Y 对照t2 -Y 对照t1 ).

[0050] Relative decrease in plant biomass (RDPB) = (Y 对照t2 -Y 盐t2 ) / Y 对照t2 .

[0051] In the above formula, Y is the measured value of plant height, stem diameter, fresh weight or dry weight; t1 and t2 are the starting and ending time points of the experiment, i.e. t2-t1 is the treatment time, the unit of time is day; salt and control are the values under salt treatment and without salt treatment, respectively.

[0052] (iii) Analysis of experimental results

[0053] In this experimental case, 350 mM NaCl solution was used for 18 days. Figure 1 The growth of plants sprayed with distilled water and sericin solution on the front of leaves, Figure 1 Middle A: spraying distilled water on the upper surface of leaves, HS1; Figure 1 Middle B is spraying sericin solution on the upper side of leaves, DS. Figure 2 For the measurement and analysis of various growth indicators.

[0054] Under salt treatment conditions, compared with those sprayed with distilled water, the plants sprayed with sericin solution grew better and were visibly taller. Figure 1 According to the measured data analysis, the relative growth rate of plant height of plants sprayed with sericin solution was significantly higher than that of plants sprayed with distilled water. Figure 2 As shown in A; the relative growth rate of stem diameter is significantly higher than that of the distilled water spraying group. Figure 2 As shown in C; the salt tolerance index of plant height and stem diameter were significantly higher than that of the distilled water spraying group. Figure 2 Middle B and Figure 2 As shown in D.

[0055] The root fresh weight and dry weight of the plants sprayed with sericin solution were significantly higher than those of the group sprayed with distilled water. Figure 2 Zhong E and Figure 2 As shown in G. Figure 2 As shown in Figure F, the relative reduction rate of biomass calculated by root fresh weight was significantly lower than that of the group sprayed with distilled water, indicating that spraying sericin solution weakened the effect of salt stress on plant root fresh weight biomass. Figure 2 As shown in Figure H, the relative reduction rate of biomass calculated based on root dry weight was significantly lower than that of the group sprayed with distilled water, and showed a negative average value, indicating that spraying sericin solution promoted the growth of root dry weight biomass under salt stress.

[0056] The aboveground fresh weight and dry weight of plants sprayed with sericin solution were significantly higher than those of the group sprayed with distilled water. Figure 2 Zhong I and Figure 2 As shown in K. Figure 2 J and Figure 3 As shown in middle L, the relative reduction rate of biomass calculated by fresh weight and dry weight of aboveground parts was significantly lower than that of the group sprayed with distilled water, indicating that spraying sericin solution alleviated the effect of salt stress on the aboveground biomass of plants.

[0057] The experimental results show that spraying sericin solution on the front of leaves can improve the salt tolerance of plants and enhance their growth under salt stress, which is manifested in an increase in plant height, stem thickness and biomass.

[0058] Experiment 2

[0059] An experiment was conducted to verify the ability of spraying sericin solution on the back of leaves to improve the salt tolerance of plants.

[0060] (I) Preparation of experimental materials

[0061] The experimental materials were prepared as in Experiment 1.

[0062] (II) Experimental design

[0063] The experimental design was the same as in Example 7, except that the spraying treatment used back leaf spraying, i.e. back leaf spraying of distilled water, marked as HS2, and back leaf spraying of sericin solution, marked as BS.

[0064] (III) Analysis of experimental results

[0065] The plants were treated with 350 mM NaCl solution for 18 days. Figure 3 The growth of the plants sprayed with distilled water and sericin solution on the back of the leaves was observed. Among them, Figure 3 A is back leaf spraying of distilled water, HS2; Figure 4 B is back leaf spraying of sericin solution, BS. Figure 3 The growth indexes were measured and analyzed.

[0066] As shown in Table 1, under the salt treatment condition, the growth of the plants sprayed with distilled water and sericin solution was not significantly different, but according to the measured data analysis, the relative growth rate of the plant height of the plants sprayed with sericin solution was significantly higher than that of the plants sprayed with distilled water, as shown in Table 2, while the relative growth rate of the stem diameter was not significantly different from that of the plants sprayed with distilled water, as shown in Table 3. Combined with the analysis of the plants grown under normal conditions, the salt tolerance indexes of the plant height and stem diameter were significantly higher than those of the plants sprayed with distilled water, as shown in Table 4 and Table 5. Figure 4 Figure 4 Figure 4 Figure 4

[0067] Compared with the plants sprayed with distilled water, the plants sprayed with sericin solution had no significant difference in root fresh weight and dry weight, as shown in Table 6 and Table 7. The relative reduction rate of the biomass calculated from the root fresh weight and dry weight also had no significant difference, as shown in Table 8 and Table 9, indicating that back leaf spraying of sericin solution had no significant effect on the root fresh weight and biomass of the plants under salt stress. Figure 4 Figure 4 Figure 4

[0068] As shown in Table 10, J, I, K and L, although the relative reduction rate of the biomass of the above-ground fresh weight of the plants sprayed with sericin solution was significantly lower than that of the plants sprayed with distilled water, the relative reduction rates of the fresh weight, dry weight and root dry weight of the plants sprayed with sericin solution had no significant difference from those of the plants sprayed with distilled water. ​

[0069] ​​​​​​​​The experimental results show that the improvement of the salt tolerance of the plant by spraying the sericin solution on the back of the leaves is limited, and cannot significantly promote the improvement of multiple growth indexes of the plant under salt stress.

[0070] The results of the experiments 1 and 2 show that under the condition of salt stress, the salt tolerance of the plant can be significantly improved by spraying the sericin solution on the front of the leaves, and the sericin solution can be used as a component of a spraying agent for improving the salt tolerance of crops planted in saline-alkali land.

[0071] The technical features of the above-described embodiments can be combined arbitrarily, and to make the description concise, all possible combinations of the technical features in the above-described embodiments are not described, however, as long as the combinations of the technical features do not exist contradictory, they should be considered as the scope of the description.

[0072] The above-described embodiments only express several implementation manners of the present application, the description is more specific and detailed, but it should not be understood as the limitation of the patent scope of the present application. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, several modifications and improvements can be made, which belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. Use of sericin solution for improving salt tolerance in plants, characterized in that, The concentration of the sericin solution is 0.1g / L-1g / L; the plant is tomato. The specific application method is: spraying sericin solution on the front of leaves, and the spraying amount of each plant is 1mL-2mL.

2. Use according to claim 1, wherein The concentration of the sericin solution is 0.2g / L.

3. The use according to claim 1, wherein The improvement of plant salt tolerance is the increase of plant height, stem diameter and biomass under salt stress environment.

4. The use according to claim 3, wherein the compound is ###0002### The salt stress environment refers to a salt stress environment with a sodium chloride concentration of 100mM-500mM.

5. The use according to claim 1, wherein The preparation method of the sericin solution is: taking silkworm cocoons, boiling in water to dissolve sericin, removing undissolved silk fibroin, and obtaining sericin solution. The ratio of clean silkworm cocoons to water is 5g-10g:200mL.

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