High-speed centrifugal machine for protein extraction

Abstract

The invention relates to the technical field of protein extraction, in particular to a high-speed centrifugal machine for protein extraction, which comprises a centrifugal rotating frame, an anti-pollution adsorption pad and a limiting storage seat, the centrifugal rotating frame is installed at the axis position in the high-speed centrifugal machine, a limiting containing plate is installed at the top of the centrifugal rotating frame, a plurality of rotating adjusting heads are distributed on the outer wall of the limiting containing plate at equal intervals, a linkage pushing and pressing mechanism is installed at the bottom of the limiting containing plate, and an adjusting cover plate is installed at the top of the limiting containing plate. The adjusting cover plate is used for adjusting and controlling the linkage pushing and pressing mechanism; the anti-pollution adsorption pad is mounted between the limiting placement plate and the adjusting cover plate, and the adsorption ends of the anti-pollution adsorption pad are distributed on the rotary adjusting head; according to the high-speed centrifugal machine, the protein extraction efficiency and the extraction quality can be effectively improved, and meanwhile the maintenance cost of the high-speed centrifugal machine is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of protein extraction, in particular to a high-speed centrifuge used for protein extraction. Background Art

[0002] In protein research, centrifugation is often used for cell precipitation, removal of impurities after cell lysis, and preliminary separation of proteins. High-speed refrigerated centrifuges can effectively separate proteins in cell extracts or cell culture supernatants, and separate proteins from other cell components (such as cell membranes, nucleic acids, and small molecules). This is the most basic step in protein research and provides the necessary samples for subsequent further research and purification.

[0003] Chinese patent number CN218755520U discloses an efficient protein extraction device suitable for a high-speed centrifuge. The centrifuge tube clamp is located above the waste liquid receiving tank and the bottom end is inserted into the waste liquid receiving tank. Several placement holes are formed near the top of the centrifuge tube clamp. The outer wall of the centrifuge tube clamp is provided with a buckle 1, and the outer wall of the waste liquid receiving tank is provided with a buckle 2. The bottom of the waste liquid receiving tank is concave to form a threaded connection groove. The buckle of the device ensures that the centrifuge tube clamp and the waste liquid receiving tank can be centrifuged stably at the same frequency. The frosted surface makes the centrifuge tube column in good contact with the centrifuge tube clamp, which guarantees the safety of the centrifuge experiment to the greatest extent. The waste liquid receiving tank can accept all the waste liquid generated by the complete experiment, avoiding multiple removal of the centrifuge tube for pouring liquid and preventing waste liquid from contaminating the tube mouth. The waste liquid surface does not contact the centrifuge tube column, which greatly avoids waste liquid contamination of the extracted protein, thereby improving the yield of the target product of the experiment. The process of protein extraction experiment is simplified, and the protein extraction efficiency is greatly improved.

[0004] The waste liquid receiving tank of the above device can receive all the waste liquid generated by the complete experiment, but when the waste liquid receiving tank is full, the centrifuge tube clamp and the waste liquid receiving tank need to be disassembled. This process is cumbersome and affects the efficiency of the experiment. If there is residual waste liquid at the mouth of the centrifuge tube, placing it directly in the clamp may cause the waste liquid to contaminate the surface of the clamp during the centrifugation process, requiring regular cleaning, increasing maintenance costs and time. Excessive waste liquid on the surface of the centrifuge tube may splash when rotating at high speed, contaminating the internal space of the centrifuge, which not only affects the cleanliness of the equipment, but also interferes with the accuracy and safety of subsequent experiments. Summary of the invention

[0005] In view of the above problems, a high-speed centrifuge for protein extraction is provided. The cooperation of a centrifugal rotating frame, an anti-pollution adsorption pad and a limiting storage seat can effectively improve the protein extraction efficiency and extraction quality, while reducing the maintenance cost of the high-speed centrifuge.

[0006] In order to solve the problems of the prior art, the present invention provides a high-speed centrifuge for protein extraction, comprising a centrifugal rotating frame, an anti-pollution adsorption pad and a limit storage seat; the centrifugal rotating frame is installed at the axial position inside the high-speed centrifuge, the centrifugal rotating frame is transmission-connected with the output end of the high-speed centrifuge, a limit placement plate is installed on the top of the centrifugal rotating frame, a plurality of rotation adjustment heads are evenly distributed on the outer wall of the limit placement plate, a linkage pushing mechanism is installed at the bottom of the limit placement plate, the linkage pushing mechanism is provided with a plurality of pushing ends, the plurality of pushing ends of the linkage pushing mechanism respectively correspond to the plurality of rotation adjustment heads one by one, an adjustment cover is installed on the top of the limit placement plate, and the adjustment cover is used to control the linkage pushing mechanism; the anti-pollution adsorption pad is installed between the limit placement plate and the adjustment cover, and the adsorption ends of the anti-pollution adsorption pad are distributed on the rotation adjustment heads; the limit storage seat is arranged on the side of the high-speed centrifuge.

[0007] Preferably, a conical guiding surface is provided on the top of the limiting placement plate, a limiting guide rail is provided on the top of the limiting placement plate, the limiting guide rail is arranged at the center position of the limiting placement plate, and a plurality of limiting snap-ons are provided inside the limiting guide rail.

[0008] Preferably, the rotating adjustment head is rotatably connected to the limiting placement plate, a first insertion hole is provided at the bottom of the rotating adjustment head, and a limiting plate is provided at the edge of the rotating adjustment head.

[0009] Preferably, the linkage pushing mechanism includes a plurality of telescopic adjustment rods slidably installed at the bottom of the limit placement plate, a first spring is installed between the telescopic adjustment rod and the limit placement plate, a resistance roller is installed at one end of the telescopic adjustment rod, a first transmission rod is rotatably installed at one end of the telescopic adjustment rod away from the resistance roller, a second transmission rod is rotatably installed at one end of the first transmission rod away from the telescopic adjustment rod, and an end of the second transmission rod away from the first transmission rod is fixedly connected to the rotating adjustment head, the linkage pushing mechanism also includes a synchronous driving wheel installed on the limit placement plate, a plurality of pushing bevel teeth are provided on the synchronous driving wheel, the inclined surface of the pushing bevel teeth is in contact with the resistance roller, and a docking through hole is provided on the top of the synchronous driving wheel.

[0010] Preferably, the adjusting cover is provided with a plurality of mounting rails, and movable blocks are slidably installed inside the mounting rails, and a limit block is provided on the side of the movable block, and the limit block matches the limit bayonet, and a pressure regulating block is provided on the top of the movable block, and a second spring is installed between the movable block and the mounting rail, and a limit plug is also provided on the adjusting cover, and the limit plug matches the docking through hole.

[0011] Preferably, the anti-pollution adsorption pad consists of an annular mounting plate and a bending adsorption head, a conical guide sleeve is provided on the top of the annular mounting plate, a plurality of pull-out buckles are provided on the annular mounting plate, a plurality of bending adsorption heads are provided, and the bending adsorption heads are evenly spaced and distributed on the outside of the annular mounting plate, and the bending adsorption heads correspond one-to-one to the rotating adjustment heads.

[0012] Preferably, a plurality of shielding plates are provided on the bending adsorption head, an adsorption bottom layer and a second through hole are provided on the bottom of the bending adsorption head, and a rubber sealing ring is provided on the second through hole.

[0013] Preferably, a first adsorption layer is disposed on the upper layer of the adsorption bottom layer, a second adsorption layer is disposed on the bottom of the first adsorption layer, a permeable membrane is disposed between the first adsorption layer and the second adsorption layer, and a plurality of permeable holes are disposed on the permeable membrane.

[0014] Preferably, a top plate is provided on the top of the position-limiting storage seat, and a positioning adsorption groove is provided below the top plate.

[0015] Preferably, a limiting groove is provided on the top plate, a plurality of third through holes are provided on the limiting groove, an annular adsorption layer is installed inside the limiting groove, a plurality of fourth through holes are provided on the annular adsorption layer, a fixing spring is also installed on the limiting groove, and the fixing spring is used to fix the annular adsorption layer.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention significantly improves the operating efficiency of the protein extraction experiment by introducing the design of the anti-pollution adsorption pad and the linkage pushing mechanism. During the centrifugation process, the anti-pollution adsorption pad can fit the centrifuge column tightly, effectively preventing the splashing of residual waste liquid at the tube mouth, and avoiding the interruption of the experiment caused by waste liquid pollution. At the same time, the coordinated use of the linkage pushing mechanism and the rotating adjustment head makes the loading and unloading process of the centrifuge column simpler and faster, shortens the experimental preparation and cleaning time, and improves the overall experimental efficiency.

[0017] 2. The use of the anti-pollution adsorption pad of the present invention is a major innovation in the anti-pollution aspect of the present device. The anti-pollution adsorption pad can efficiently adsorb the waste liquid remaining at the mouth of the centrifuge column, effectively avoiding splashing and contamination of the waste liquid during the centrifugation process, and protecting the cleanliness of the internal space of the centrifuge. In addition, since the anti-pollution adsorption pad is easy to disassemble and replace, it can significantly reduce the frequency and cost of equipment maintenance caused by waste liquid contamination, extend the service life of the centrifuge, and reduce the operating costs of the laboratory.

[0018] 3. The present invention further optimizes the process of protein extraction experiments through the design of the limited storage seat and the adjustment cover. The limited storage seat can vertically limit multiple centrifuge columns, which is convenient for staff to quickly position and operate when adding protein extraction reagents, thereby improving the accuracy and efficiency of reagent addition. At the same time, the installation and disassembly process of the adjustment cover is simple and convenient, and can trigger the linkage pushing mechanism to adjust the angle, making the loading and unloading of the centrifuge columns safer and more stable. In addition, the effective use of the anti-pollution adsorption pad avoids the contamination of the internal space of the centrifuge by waste liquid, thereby ensuring the accuracy and safety of subsequent experiments, and providing a strong guarantee for the smooth progress of scientific research. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a three-dimensional schematic diagram of a high-speed centrifuge for protein extraction according to the present invention.

[0020] Figure 2 This is a three-dimensional schematic diagram of a part of the structure of a high-speed centrifuge for protein extraction according to the present invention. Figure 1 .

[0021] Figure 3 This is a three-dimensional schematic diagram of a part of the structure of a high-speed centrifuge for protein extraction according to the present invention. Figure 2 .

[0022] Figure 4 yes Figure 3 A partial enlarged view of point A in the middle.

[0023] Figure 5 The present invention is an exploded view of a part of the mechanism of a high-speed centrifuge for protein extraction.

[0024] Figure 6 It is a stereoscopic schematic diagram of an adjusting cover plate in a high-speed centrifuge for protein extraction according to the present invention.

[0025] Figure 7 The invention is a three-dimensional schematic diagram of a limiting storage seat, an anti-pollution adsorption pad and a centrifugal column in a high-speed centrifuge for protein extraction.

[0026] Figure 8 The present invention is an exploded view of a position limiting storage seat, an anti-pollution adsorption pad and a centrifugal column in a high-speed centrifuge for protein extraction.

[0027] Fig. 9 The present invention is a three-dimensional schematic diagram of an anti-pollution adsorption pad in a high-speed centrifuge for protein extraction.

[0028] Fig.10 yes Fig. 9 A partial enlarged view of point B in the middle.

[0029] Fig.11 It is a planar cross-sectional view of a bent adsorption head in a high-speed centrifuge for protein extraction according to the present invention.

[0030] The numbers in the figure are: 1. High-speed centrifuge; 2. Centrifugal rotating frame; 21. Limiting plate; 211. Conical guide surface; 212. Limiting guide rail; 213. Limiting bayonet; 22. Rotating adjustment head; 221. Limiting plate; 222. First insertion hole; 23. Linkage pushing mechanism; 231. Telescopic adjustment rod; 232. First spring; 233. Resisting roller; 234. First transmission rod; 235. Second transmission rod; 236. Synchronous driving wheel; 237. Pushing helical teeth; 238. Docking insertion hole; 24. Adjusting cover plate; 241. Installing slide rail; 242. Limiting plug block; 243. Movable card block; 2431 , limit block; 2432, pressure regulating block; 244, second spring; 3, anti-pollution adsorption pad; 31, annular mounting plate; 311, conical guide sleeve; 312, pull-out buckle; 32, bending adsorption head; 321, shielding plate; 322, adsorption bottom layer; 3221, first adsorption layer; 3222, permeable membrane; 3223, second adsorption layer; 323, rubber sealing ring; 4, limit storage seat; 41, placement top plate; 411, third insertion hole; 412, limit groove; 413, annular adsorption layer; 414, fourth insertion hole; 415, fixing retaining spring; 42, positioning adsorption groove; 5, centrifugal column. DETAILED DESCRIPTION

[0031] In order to further understand the features, technical means, specific objectives and functions of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0032] See also Figures 1 to 11 As shown, a high-speed centrifuge for protein extraction comprises a centrifugal rotating frame 2, an anti-pollution adsorption pad 3 and a limit storage seat 4; the centrifugal rotating frame 2 is installed at the axial position inside the high-speed centrifuge 1, the centrifugal rotating frame 2 is transmission-connected with the output end of the high-speed centrifuge 1, a limit placement plate 21 is installed on the top of the centrifugal rotating frame 2, a plurality of rotating adjustment heads 22 are evenly distributed on the outer wall of the limit placement plate 21, a linkage pushing mechanism 23 is installed at the bottom of the limit placement plate 21, the linkage pushing mechanism 23 is provided with a plurality of pushing ends, and the plurality of pushing ends of the linkage pushing mechanism 23 correspond to the plurality of rotating adjustment heads 22 one by one, an adjustment cover plate 24 is installed on the top of the limit placement plate 21, and the adjustment cover plate 24 is used to regulate the linkage pushing mechanism 23; the anti-pollution adsorption pad 3 is installed between the limit placement plate 21 and the adjustment cover plate 24, and the adsorption ends of the anti-pollution adsorption pad 3 are distributed on the rotating adjustment heads 22; the limit storage seat 4 is arranged on the side of the high-speed centrifuge 1.

[0033] First, the staff correctly installs the anti-pollution adsorption pad 3 on the limiting placement plate 21 and each rotating adjustment head 22. This step ensures that the anti-pollution adsorption pad 3 can fit tightly against the rotating adjustment head 22 to prepare for the subsequent waste liquid adsorption. Then, the adjustment cover 24 is installed on the limiting placement plate 21. During the installation process, the adjustment cover 24 not only plays a fixing role, but also triggers the linkage pushing mechanism 23. After the linkage pushing mechanism 23 is triggered, its multiple pushing ends respectively push the corresponding rotating adjustment head 22 to adjust the angle. The step ensures that the centrifugal column 5 maintains a stable centrifugal angle when it is installed on the rotating adjustment head 22.

[0034] The staff sequentially passes the multiple centrifuge columns 5 containing the extract through the anti-pollution adsorption pad 3 and the rotating adjustment head 22 to ensure that the centrifuge columns 5 are firmly placed on the rotating adjustment head 22. The high-speed centrifuge 1 is started, and the centrifuge rotating frame 2 rotates accordingly to perform the initial centrifugation operation.

[0035] After the initial centrifugation, a protein extraction reagent needs to be added to the centrifuge column 5. At this time, the staff opens the high-speed centrifuge 1 and removes the adjustment cover 24. After the adjustment cover 24 is removed, the linkage push mechanism 23 is reset, driving the rotating adjustment head 22 to reset to the initial position. This step allows the anti-pollution adsorption pad 3 and the centrifuge column 5 to be easily detached from the limit placement plate 21.

[0036] The staff moves the anti-pollution adsorption pad 3 and the centrifuge column 5 as a whole to the limiting storage seat 4. The design of the limiting storage seat 4 ensures that multiple centrifuge columns 5 can be vertically limited, which is convenient for the staff to add protein extraction reagents and improves operating efficiency. After the protein extraction reagent is added, the staff uses the anti-pollution adsorption pad 3 again to synchronously put the multiple centrifuge columns 5 back to the limiting placement plate 21. During this process, the anti-pollution adsorption pad 3 continues to play its role, ensuring that the waste liquid that may remain at the mouth of the centrifuge column 5 will not drip directly into the centrifuge, but will be effectively adsorbed by the anti-pollution adsorption pad 3. Subsequently, the high-speed centrifuge 1 is started again for subsequent centrifugation operations.

[0037] During the entire centrifugation process, the anti-pollution adsorption pad 3 effectively avoids splashing and contamination of waste liquid, ensuring the cleanliness of the internal space of the centrifuge. After the experiment, the anti-pollution adsorption pad 3 can be easily disassembled and cleaned and recycled, thereby reducing maintenance costs and time, while ensuring the accuracy and safety of subsequent experiments.

[0038] The anti-pollution adsorption pad 3 is disposable and easy to use.

[0039] See also Figures 2 to 5As shown, a conical guiding surface 211 is provided on the top of the limiting placing plate 21 , a limiting guide rail 212 is provided on the top of the limiting placing plate 21 , the limiting guide rail 212 is arranged at the center position of the limiting placing plate 21 , and a plurality of limiting snap-in sockets 213 are provided inside the limiting guide rail 212 .

[0040] A conical guide surface 211 is provided on the top of the limiting placement plate 21, and the conical guide surface 211 plays a role in supporting the anti-pollution adsorption pad 3 during the assembly process. Due to the design of the conical guide surface 211, the anti-pollution adsorption pad 3 can be automatically centered during installation to ensure that it fits tightly with the rotating adjustment head 22, so that the anti-pollution adsorption pad 3 maintains stability during the centrifugal rotation process, effectively avoiding the occurrence of displacement.

[0041] During the installation process of the adjustment cover plate 24, the position limiting guide rail 212 plays a role in guiding the rotation and adjustment of the adjustment cover plate 24. Through the guidance of the position limiting guide rail 212, the adjustment cover plate 24 can be accurately and smoothly rotated to a predetermined position.

[0042] A plurality of limit snap-in slots 213 are provided inside the limit guide rail 212, and the limit snap-in slots 213 match the snap-in ends on the adjustment cover 24. When the adjustment cover 24 rotates to a predetermined position, the snap-in ends on the adjustment cover 24 will be embedded in the limit snap-in slots 213, thereby achieving snap-in positioning of the adjustment cover 24. This design not only ensures the stable installation of the adjustment cover 24, but also avoids loosening or falling off due to vibration during the centrifugation process.

[0043] See also Figures 2 to 5 As shown, the rotating adjustment head 22 is rotatably connected to the limiting placement plate 21 , a first insertion hole 222 is provided at the bottom of the rotating adjustment head 22 , and a limiting plate 221 is provided at the edge of the rotating adjustment head 22 .

[0044] The rotating adjustment head 22 is rotatably connected to the limiting placement plate 21, allowing the rotating adjustment head 22 to adjust its angle relative to the limiting placement plate 21 when subjected to external force. The first insertion hole 222 of the rotating adjustment head 22 is located at the center of the rotating adjustment head 22. The first insertion hole 222 serves as an insertion channel for the centrifugal column 5, ensuring that the centrifugal column 5 can be firmly and accurately mounted on the rotating adjustment head 22 so as to remain stable during the centrifugation process.

[0045] In addition, a limit plate 221 is provided at the edge of the rotating adjustment head 22, and the main function of the limit plate 221 is to cooperate with the installation of the anti-pollution adsorption pad 3. When the anti-pollution adsorption pad 3 is placed between the limit placement plate 21 and the rotating adjustment head 22, the limit plate 221 can effectively limit the movement of the anti-pollution adsorption pad 3, ensuring that the contact area between the anti-pollution adsorption pad 3 and the rotating adjustment head 22 maintains a tight fit, thereby preventing the anti-pollution adsorption pad 3 from being misplaced or falling off due to vibration or centrifugal force during the centrifugation process.

[0046] See also Figures 2 to 4 As shown, the linkage pushing mechanism 23 includes a plurality of telescopic adjustment rods 231 slidably installed at the bottom of the limiting placement plate 21, a first spring 232 is installed between the telescopic adjustment rod 231 and the limiting placement plate 21, a resistance roller 233 is installed at one end of the telescopic adjustment rod 231, a first transmission rod 234 is rotatably installed at one end of the telescopic adjustment rod 231 away from the resistance roller 233, a second transmission rod 235 is rotatably installed at one end of the first transmission rod 234 away from the telescopic adjustment rod 231, and an end of the second transmission rod 235 away from the first transmission rod 234 is fixedly connected to the rotating adjustment head 22, and the linkage pushing mechanism 23 also includes a synchronous driving wheel 236 installed on the limiting placement plate 21, a plurality of pushing bevel teeth 237 are provided on the synchronous driving wheel 236, the inclined surface of the pushing bevel teeth 237 is in contact with the resistance roller 233, and a docking insertion hole 238 is provided at the top of the synchronous driving wheel 236.

[0047] The linkage pushing mechanism 23 is mainly composed of a plurality of telescopic adjustment rods 231 slidably mounted on the bottom of the limiting placement plate 21, and the telescopic adjustment rod 231 is elastically connected to the limiting placement plate 21 through a first spring 232. A resisting roller 233 is installed at one end of the telescopic adjustment rod 231, and the resisting roller 233 contacts and transmits with the pushing helical teeth 237 on the synchronous driving wheel 236. The surface of the synchronous driving wheel 236 is provided with a plurality of pushing helical teeth 237, and the inclined surface of the pushing helical teeth 237 forms an effective resisting relationship with the resisting roller 233. When the adjusting cover plate 24 is mounted on the limiting placement plate 21 and rotates, it will dock with the docking insertion hole 238 of the synchronous driving wheel 236, and drive the synchronous driving wheel 236 to rotate synchronously. As the synchronous driving wheel 236 rotates, the inclined surface of the pushing helical teeth 237 will contact with each of the resisting rollers 233 in turn, and push the resisting roller 233 to move along a preset direction. The movement of the abutting roller 233 will further drive the telescopic adjustment rod 231 to slide at the bottom of the limiting placement plate 21, and compress the first spring 232 at the same time. The sliding of the telescopic adjustment rod 231 will drive the first transmission rod 234 and the second transmission rod 235 at both ends thereof to be linked. The first transmission rod 234 is connected to the telescopic adjustment rod 231 through a rotation connection, and the second transmission rod 235 is fixedly connected to the rotating adjustment head 22 through an end away from the first transmission rod 234. Therefore, when the telescopic adjustment rod 231 slides, the first transmission rod 234 and the second transmission rod 235 will act together on the rotating adjustment head 22 to push it to adjust the angle.

[0048] When the adjustment cover 24 needs to be disassembled, the first spring 232 will push the telescopic adjustment rod 231 to reset synchronously. When the telescopic adjustment rod 231 is reset, the adjustment head 22 can be rotated to reset synchronously.

[0049] See also Figures 2 to 6 As shown, the adjusting cover 24 is provided with a plurality of mounting rails 241, and movable blocks 243 are slidably installed inside the mounting rails 241. The side of the movable block 243 is provided with a limit block 2431, and the limit block 2431 matches the limit bayonet 213. A pressure regulating block 2432 is provided on the top of the movable block 243, and a second spring 244 is installed between the movable block 243 and the mounting rail 241. A limit plug 242 is also provided on the adjusting cover 24, and the limit plug 242 matches the docking through hole 238.

[0050] First, a plurality of mounting rails 241 are provided on the adjusting cover 24, and the mounting rails 241 provide a stable sliding track for the movable block 243. In the process of installing the adjusting cover 24, the staff will press the pressure regulating block 2432 synchronously, and the second spring 244 will be compressed, so that the movable block 243 and the limit block 2431 remain in a contracted state. The contracted state facilitates the smooth insertion of the movable block 243 and the limit block 2431 into the limit guide rail 212, and also enables the limit block 242 to be easily inserted into the docking insertion hole 238 of the synchronous drive wheel 236. Once the adjusting cover 24 is initially installed in place, the staff will rotate the adjusting cover 24 to a predetermined position. During the rotation process, the cooperation between the limit block 242 and the docking insertion hole 238 ensures the synchronous rotation of the adjusting cover 24 and the synchronous drive wheel 236.

[0051] When the adjustment cover 24 rotates to the specified position, the elastic force of the second spring 244 is released, pushing the movable block 243 to move along the mounting rail 241. As a result, the limit block 2431 is engaged with the limit slot 213, thereby achieving accurate positioning of the adjustment cover 24. This design not only ensures the stable installation of the adjustment cover 24, but also avoids loosening or falling off due to vibration during the centrifugation process.

[0052] In addition, the adjustment cover 24 also limits and presses the anti-pollution adsorption pad 3 during the rotation process. The pressing action ensures that the anti-pollution adsorption pad 3 is tightly fitted with the rotating adjustment head 22, thereby preventing the anti-pollution adsorption pad 3 from being dislocated or falling off due to vibration or centrifugal force during the centrifugation process.

[0053] See also Figures 2 to 9 As shown, the anti-pollution adsorption pad 3 is composed of an annular mounting plate 31 and a bending adsorption head 32. A conical guide sleeve 311 is provided on the top of the annular mounting plate 31. A plurality of pull-out buckles 312 are provided on the annular mounting plate 31. A plurality of bending adsorption heads 32 are provided, and the bending adsorption heads 32 are evenly spaced on the outside of the annular mounting plate 31. The bending adsorption heads 32 correspond one-to-one to the rotating adjustment heads 22.

[0054] A conical guide sleeve 311 is provided on the top of the annular mounting plate 31, and the conical guide sleeve 311 can closely match the conical guide surface 211 of the limiting placement plate 21 to achieve stable fit. This design not only ensures the centering and stability of the anti-pollution adsorption pad 3 during installation, but also effectively prevents displacement caused by vibration or centrifugal force during centrifugation.

[0055] The annular mounting plate 31 is provided with a plurality of pull-out buckles 312, which facilitate the staff to take and operate when installing and removing the anti-pollution adsorption pad 3. The pull-out buckles 312 make the replacement of the anti-pollution adsorption pad 3 simple and quick, reducing the difficulty of operation and time cost.

[0056] The bending adsorption heads 32 are evenly distributed on the outside of the annular mounting plate 31 and correspond one to one with the rotating adjustment heads 22. During the installation process, the bending adsorption heads 32 can effectively dock with the rotating adjustment heads 22, and the bending adsorption heads 32 are in limited contact with the centrifuge column 5. The limited contact not only ensures the stability of the centrifuge column 5 during the centrifugation process, but also effectively prevents the splashing of waste liquid. The bending adsorption heads 32 can absorb the waste liquid that may remain at the mouth of the centrifuge column 5 to prevent the waste liquid from dripping into the centrifuge. In addition, the bending adsorption heads 32 can adjust the angle along with the rotating adjustment heads 22.

[0057] See also Fig. 9 and Fig.10 As shown, a plurality of shielding plates 321 are provided on the bending adsorption head 32, an adsorption bottom layer 322 and a second through hole are provided at the bottom of the bending adsorption head 32, and a rubber sealing ring 323 is provided on the second through hole.

[0058] The bending adsorption head 32 corresponds to the rotating adjustment head 22 one by one, and ensures the stability of the centrifuge column 5 and prevents waste liquid from splashing during high-speed centrifugation. Multiple shielding plates 321 effectively block the waste liquid splashing generated by the centrifuge column 5 during the centrifugation process, thereby avoiding the waste liquid from polluting the internal space of the centrifuge.

[0059] The bottom of the bent adsorption head 32 is provided with an adsorption bottom layer 322, which has adsorption capacity and can effectively adsorb the waste liquid that may remain at the mouth of the centrifuge column 5. This design ensures that the waste liquid does not drip directly into the centrifuge, but is effectively captured by the bent adsorption head 32.

[0060] In addition, a second insertion hole is provided on the bending adsorption head 32, and the second insertion hole matches the first insertion hole 222 on the rotating adjustment head 22, providing a stable insertion channel for the centrifugal column 5. The rubber sealing ring 323 provided on the second insertion hole ensures the close fit of the centrifugal column 5 during the insertion process, and prevents the waste liquid at the tube mouth from flowing along the outer wall when the centrifugal column 5 rotates, further ensuring the stable contact between the waste liquid and the adsorption bottom layer 322, and improving the adsorption efficiency of the waste liquid.

[0061] See also Fig.10 and Fig.11 As shown, a first adsorption layer 3221 is disposed on the upper layer of the adsorption bottom layer 322, a second adsorption layer 3223 is disposed on the bottom of the first adsorption layer 3221, a permeable membrane 3222 is disposed between the first adsorption layer 3221 and the second adsorption layer 3223, and a plurality of permeable holes are disposed on the permeable membrane 3222.

[0062] The adsorption bottom layer 322 is located at the bottom of the bent adsorption head 32. The adsorption bottom layer 322 effectively adsorbs the waste liquid that may remain at the mouth of the centrifuge column 5, thereby preventing the waste liquid from dripping into the centrifuge, thereby ensuring the cleanliness and safety of the centrifugation process.

[0063] The adsorption bottom layer 322 is composed of a first adsorption layer 3221, a second adsorption layer 3223 and a permeable membrane 3222 located therebetween. The first adsorption layer 3221 is located at the upper layer, directly facing the waste liquid that may drip. The first adsorption layer 3221 has a good initial adsorption capacity and can quickly adsorb the waste liquid to prevent it from spreading.

[0064] A permeable membrane 3222 is provided between the first adsorption layer 3221 and the second adsorption layer 3223, and a plurality of permeable holes are evenly distributed on the permeable membrane 3222. The permeable holes allow the waste liquid adsorbed by the first adsorption layer 3221 to penetrate through under the action of centrifugal pressure and enter the second adsorption layer 3223. The permeable membrane 3222 plays an isolating role, preventing the waste liquid from randomly diffusing between the first adsorption layer 3221 and the second adsorption layer 3223.

[0065] The second adsorption layer 3223 is located below the first adsorption layer 3221 and has a stronger adsorption capacity. It can perform secondary adsorption on the waste liquid that penetrates from the first adsorption layer 3221 to ensure that the waste liquid is completely captured. This double-layer adsorption design not only improves the adsorption efficiency of the waste liquid, but also enhances the stability and durability of the adsorption bottom layer 322.

[0066] See also Figure 1 , Figure 7 and Figure 8 As shown, a placement top plate 41 is provided on the top of the position-limiting storage seat 4 , and a positioning adsorption groove 42 is provided below the placement top plate 41 .

[0067] The limiting storage seat 4 is arranged beside the high-speed centrifuge 1. After the initial centrifugation, the limiting storage seat 4 provides a stable vertical limiting space for the centrifuge column 5, so that the staff can add protein extraction reagents.

[0068] A placement top plate 41 is provided on the top of the limited storage seat 4, and the placement top plate 41 is used to horizontally support the centrifuge column 5 carried by the anti-pollution adsorption pad 3. The design of the placement top plate 41 ensures the stability and safety of the centrifuge column 5 during movement, and avoids waste liquid splashing or damage to the centrifuge column 5 due to shaking or tilting.

[0069] A positioning adsorption groove 42 is provided below the top plate 41, and an adsorption hole is provided inside the positioning adsorption groove 42, which can be in close contact with the outer wall of the centrifuge column 5, thereby effectively positioning the centrifuge column 5. Not only does it ensure the vertical limit of the centrifuge column 5 on the limit storage seat 4, but it also prevents the centrifuge columns 5 from colliding or tilting with each other, further improving the safety and efficiency of the operation.

[0070] After the initial centrifugation, the staff needs to add protein extraction reagent to the centrifuge column 5. At this time, first remove the adjustment cover 24, reset the linkage push mechanism 23, and drive the rotating adjustment head 22 to reset to the initial position. Then, the staff will move the anti-pollution adsorption pad 3 carrying the centrifuge column 5 as a whole to the placement top plate 41 of the limited storage seat 4. In this process, the centrifuge column 5 vertically passes through the placement top plate 41 and is in close contact with the adsorption hole in the positioning adsorption groove 42, thereby realizing the vertical limitation of the centrifuge column 5.

[0071] The design of the limited storage seat 4 enables the staff to quickly and accurately add protein extraction reagents to the centrifuge column 5 without worrying about the shaking or tilting of the centrifuge column 5. In addition, the limited storage seat 4 also facilitates the staff to observe and record the centrifuge column 5 after adding the reagent, further improving the accuracy and efficiency of the experiment.

[0072] See also Figure 7 and Figure 8 As shown, a limiting groove 412 is also provided on the top plate 41, a plurality of third through holes 411 are provided on the limiting groove 412, an annular adsorption layer 413 is installed inside the limiting groove 412, a plurality of fourth through holes 414 are provided on the annular adsorption layer 413, a fixing spring 415 is also installed on the limiting groove 412, and the fixing spring 415 is used to fix the annular adsorption layer 413.

[0073] The top plate 41 is used to support the entire centrifugal column 5 carried by the anti-pollution adsorption pad 3. After the initial centrifugation, when the staff needs to add a protein extraction reagent to the centrifugal column 5, the top plate 41 plays a vital role.

[0074] The limiting groove 412 is specially used for installing the annular adsorption layer 413. The annular adsorption layer 413 maintains a certain gap with the outer wall of the centrifugal column 5 through a plurality of fourth insertion holes 414, but can be in close contact, so as to effectively absorb the waste liquid that may drip and prevent the waste liquid from directly falling on the placement top plate 41. Not only does it keep the placement top plate 41 clean, but it also avoids the possible contamination of the waste liquid to subsequent experiments.

[0075] The limiting groove 412 is also provided with a plurality of third insertion holes 411, which correspond to the fourth insertion holes 414 and together provide an insertion channel for the centrifugal column 5. In this way, the centrifugal column 5 can vertically pass through the placement top plate 41 and be effectively supported and positioned by the annular adsorption layer 413.

[0076] In addition, in order to fix the annular adsorption layer 413, a fixing spring 415 is installed on the limiting groove 412. The fixing spring 415 firmly limits the annular adsorption layer 413 in the limiting groove 412 through its elastic characteristics to prevent it from being displaced or falling off during the experiment. This design not only ensures the stability of the annular adsorption layer 413, but also facilitates the staff to disassemble and maintain it when necessary.

[0077] Specific working principle: First, the staff correctly installs the anti-pollution adsorption pad 3 on the limiting placement plate 21 and each rotating adjustment head 22. This step ensures that the anti-pollution adsorption pad 3 can fit tightly against the rotating adjustment head 22 to prepare for the subsequent waste liquid adsorption. Then, the adjustment cover 24 is installed on the limiting placement plate 21. During the installation process, the adjustment cover 24 not only plays a fixing role, but also triggers the linkage pushing mechanism 23. After the linkage pushing mechanism 23 is triggered, its multiple pushing ends respectively push the corresponding rotating adjustment head 22 to adjust the angle. The step ensures that the centrifugal column 5 maintains a stable centrifugal angle when it is installed on the rotating adjustment head 22.

[0078] The staff sequentially passes the multiple centrifuge columns 5 containing the extract through the anti-pollution adsorption pad 3 and the rotating adjustment head 22 to ensure that the centrifuge columns 5 are firmly placed on the rotating adjustment head 22. The high-speed centrifuge 1 is started, and the centrifuge rotating frame 2 rotates accordingly to perform the initial centrifugation operation.

[0079] After the initial centrifugation, a protein extraction reagent needs to be added to the centrifuge column 5. At this time, the staff opens the high-speed centrifuge 1 and removes the adjustment cover 24. After the adjustment cover 24 is removed, the linkage push mechanism 23 is reset, driving the rotating adjustment head 22 to reset to the initial position. This step allows the anti-pollution adsorption pad 3 and the centrifuge column 5 to be easily detached from the limit placement plate 21.

[0080] The staff moves the anti-pollution adsorption pad 3 and the centrifuge column 5 as a whole to the limiting storage seat 4. The design of the limiting storage seat 4 ensures that multiple centrifuge columns 5 can be vertically limited, which is convenient for the staff to add protein extraction reagents and improves operating efficiency. After the protein extraction reagent is added, the staff uses the anti-pollution adsorption pad 3 again to synchronously put the multiple centrifuge columns 5 back to the limiting placement plate 21. During this process, the anti-pollution adsorption pad 3 continues to play its role, ensuring that the waste liquid that may remain at the mouth of the centrifuge column 5 will not drip directly into the centrifuge, but will be effectively adsorbed by the anti-pollution adsorption pad 3. Subsequently, the high-speed centrifuge 1 is started again for subsequent centrifugation operations.

[0081] During the entire centrifugation process, the anti-pollution adsorption pad 3 effectively avoids splashing and contamination of waste liquid, ensuring the cleanliness of the internal space of the centrifuge. After the experiment, the anti-pollution adsorption pad 3 can be easily disassembled and cleaned and recycled, thereby reducing maintenance costs and time, while ensuring the accuracy and safety of subsequent experiments.

[0082] The above embodiments only express one or several implementation modes of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the protection scope of the present invention. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the attached claims.

Claims

1. A high-speed centrifuge for protein extraction, characterized in that: It comprises a centrifugal rotating frame (2), an anti-pollution adsorption pad (3) and a limited storage seat (4); The centrifugal rotating frame (2) is installed at the axis position inside the high-speed centrifuge (1). The centrifugal rotating frame (2) is drivingly connected to the output end of the high-speed centrifuge (1). A limit placement plate (21) is installed on the top of the centrifugal rotating frame (2). A plurality of rotating adjustment heads (22) are evenly spaced on the outer wall of the limit placement plate (21). A linkage pushing mechanism (23) is installed at the bottom of the limit placement plate (21). The linkage pushing mechanism (23) is provided with a plurality of pushing ends. The plurality of pushing ends of the linkage pushing mechanism (23) correspond to the plurality of rotating adjustment heads (22) one by one. An adjustment cover plate (24) is installed on the top of the limit placement plate (21). The adjustment cover plate (24) is used to control the linkage pushing mechanism (23). The anti-pollution adsorption pad (3) is installed between the limit placement plate (21) and the adjustment cover plate (24), and the adsorption end of the anti-pollution adsorption pad (3) is distributed on the rotating adjustment head (22); The limit storage seat (4) is arranged beside the high-speed centrifuge (1).

2. A high-speed centrifuge for protein extraction according to claim 1, characterized in that: A conical guide surface (211) is provided on the top of the position limiting placement plate (21), a position limiting guide rail (212) is provided on the top of the position limiting placement plate (21), the position limiting guide rail (212) is arranged at a central position of the position limiting placement plate (21), and a plurality of position limiting snap-in openings (213) are provided inside the position limiting guide rail (212).

3. A high-speed centrifuge for protein extraction according to claim 1, characterized in that: The rotating adjustment head (22) is rotatably connected to the limiting placement plate (21); a first insertion hole (222) is provided at the bottom of the rotating adjustment head (22); and a limiting plate (221) is provided at the edge of the rotating adjustment head (22).

4. A high-speed centrifuge for protein extraction according to claim 2, characterized in that: The linkage pushing mechanism (23) comprises a plurality of telescopic adjustment rods (231) slidably mounted on the bottom of the limit placement plate (21); a first spring (232) is mounted between the telescopic adjustment rod (231) and the limit placement plate (21); a resisting roller (233) is mounted on one end of the telescopic adjustment rod (231); a first transmission rod (234) is rotatably mounted on one end of the telescopic adjustment rod (231) away from the resisting roller (233); and an end of the first transmission rod (234) away from the telescopic adjustment rod (231) is away from the A second transmission rod (235) is rotatably mounted, and one end of the second transmission rod (235) away from the first transmission rod (234) is fixedly connected to the rotation regulating head (22). The linkage pushing mechanism (23) further comprises a synchronous driving wheel (236) mounted on the limiting placement plate (21), and a plurality of pushing helical teeth (237) are provided on the synchronous driving wheel (236). The inclined surfaces of the pushing helical teeth (237) are in contact with the abutting roller (233), and a docking insertion hole (238) is provided on the top of the synchronous driving wheel (236).

5. A high-speed centrifuge for protein extraction according to claim 4, characterized in that: A plurality of mounting rails (241) are provided on the adjustment cover (24), and movable blocks (243) are slidably installed inside the mounting rails (241). A limit block (2431) is provided on the side of the movable block (243), and the limit block (2431) matches the limit bayonet (213). A pressure regulating block (2432) is provided on the top of the movable block (243), and a second spring (244) is installed between the movable block (243) and the mounting rail (241). A limit plug (242) is also provided on the adjustment cover (24), and the limit plug (242) matches the docking insertion hole (238).

6. A high-speed centrifuge for protein extraction according to claim 1, characterized in that: The anti-pollution adsorption pad (3) is composed of an annular mounting plate (31) and a bent adsorption head (32); a conical guide sleeve (311) is provided on the top of the annular mounting plate (31); a plurality of draw-out buckles (312) are provided on the annular mounting plate (31); a plurality of bent adsorption heads (32) are provided, and the bent adsorption heads (32) are evenly spaced and distributed outside the annular mounting plate (31); the bent adsorption heads (32) correspond one to one with the rotating adjustment heads (22).

7. A high-speed centrifuge for protein extraction according to claim 6, characterized in that: A plurality of shielding plates (321) are provided on the bending adsorption head (32), an adsorption bottom layer (322) and a second insertion hole are provided at the bottom of the bending adsorption head (32), and a rubber sealing ring (323) is provided on the second insertion hole.

8. A high-speed centrifuge for protein extraction according to claim 7, characterized in that: A first adsorption layer (3221) is provided on the upper layer of the adsorption bottom layer (322); a second adsorption layer (3223) is provided on the bottom of the first adsorption layer (3221); a permeable membrane (3222) is provided between the first adsorption layer (3221) and the second adsorption layer (3223); and a plurality of permeable holes are provided on the permeable membrane (3222).

9. A high-speed centrifuge for protein extraction according to claim 1, characterized in that: A placement top plate (41) is provided on the top of the position-limiting storage seat (4), and a positioning adsorption groove (42) is provided below the placement top plate (41).

10. A high-speed centrifuge for protein extraction according to claim 2, characterized in that: A limiting groove (412) is also provided on the placement top plate (41), a plurality of third insertion holes (411) are provided on the limiting groove (412), an annular adsorption layer (413) is installed inside the limiting groove (412), a plurality of fourth insertion holes (414) are provided on the annular adsorption layer (413), and a fixing spring (415) is also installed on the limiting groove (412), and the fixing spring (415) is used to fix the annular adsorption layer (413).

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