Preparation method of dithiothreitol
Through the steps of epoxidation, substitution and hydrolysis reaction, the synthesis route of dithiothreitol is simplified, the toxicity and safety hazards of raw materials and reactants in the prior art are solved, and efficient synthesis suitable for industrial production is achieved.
CN119684174APending Publication Date: 2025-03-25SUZHOU HIGHFINE BIOTECH
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Patent Information
- Application Number
- CN202411867101.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-18
- Publication Date
- 2025-03-25
AI Technical Summary
Technical Problem
The existing synthesis route of dithiothreitol is complex, and the raw materials and reactants used have toxicity and safety risks, and is not suitable for industrial production.
Method used
1,4-dibromo-2-butene and peroxy acid are epoxidized to produce 1,4-dibromo-2-epoxidized butene, and then substituted with thioacetate, and finally hydrolyzed in the presence of alkali to obtain dithiothreitol.
Benefits of technology
The synthesis route has been simplified, the raw materials are safe and non-toxic, and impurities are easy to remove, making them suitable for industrial production.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention provides a preparation method of dithiothreitol, which comprises the following steps: step S1, carrying out epoxidation reaction on 1, 4-dibromo-2-butene and peroxy acid to generate 1, 4-dibromo-2-epoxidized butene; s2, carrying out a substitution reaction on the 1, 4-dibromo-2-epoxidized butylene and thioacetate, so as to generate 1, 4-diacetyl thio-2-epoxidized butylene, and carrying out a reaction on the 1, 4-dibromo-2-epoxidized butylene and thioacetate so as to generate 1, 4-diacetyl thio-2-epoxidized butylene; and S3, carrying out a hydrolysis reaction on the 1, 4-diacetyl thio-2-epoxidized butylene, so as to generate the dithiothreitol. According to the preparation method disclosed by the embodiment of the invention, 1, 4-dibromo-2-butene is used as a starting raw material, the epoxidation reaction is firstly carried out, then thioacetate is used for replacing bromine at the tail end, and finally the hydrolysis reaction is carried out to obtain the target product dithiothreitol, so that the whole synthesis route is short, the raw material is safe and non-toxic, impurities are easy to remove, and the preparation method is suitable for industrial production.
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